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95results about How to "Large biomass" patented technology

Cladosporium sp. Fungus YS37 and application thereof in promoting rice growth

The invention discloses a cladosporium sp. Fungus YS37 and application thereof in promoting rice growth, and belongs to the technical field of microorganisms. The cladosporioides sp. Fungus YS37 is a new species separated from the root system of wild rice, is named as Parasarocladium oryzae YS37, is preserved in the China Center for Type Culture Collection (CCTCC), and has a preservation number of CCTCC NO: M 20242286, and the cladosporioides sp. Fungus YS37 is a new species which is separated from the root system of wild rice and is named as Parasarocladium oryzae YS37. The Parasarocladium oryzae YS37 strain can be used for preparing a biological bacterial fertilizer, and when the Parasarocladium oryzae YS37 strain or a fermentation product of the Parasarocladium oryzae YS37 strain is applied to a rice root system, the growth of rice can be effectively promoted, and the plant biomass is increased. The invention provides a new way for application of microorganisms to development of crop production.
Owner:ZHEJIANG UNIV

Increasing leaf biomass and nitrogen use efficiency by modulation of NTP2

Disclosed herein is a mutant, non-naturally occurring or transgenic plant or plant part having reduced or inhibited expression or activity of NtNTP2-T, or reduced or inhibited expression or activity of NtNTP2-T and NtNTP2-S, the NtNTP2-T and NtNTP2-S comprising or consisting of: (i) a polynucleotide sequence of NtNTP2-S comprising at least one of a nucleotide sequence of NtNTP2-S and a nucleotide sequence of NtNTP2-S comprising at least one of a nucleotide sequence of NtNTP2-S, a nucleotide sequence of NtNTP2-S, a nucleotide sequence of NtNTP2-S, and a nucleotide sequence of NtNTP2-S comprising at least one of a nucleotide sequence of NtNTP2-S, and a nucleotide sequence of NtNTP2-S; a nucleotide sequence comprising, consisting or consisting essentially of a sequence having at least 70% sequence identity to SEQ ID NO: 3; or (ii) an NtNTP2-T polynucleotide sequence comprising, consisting or consisting essentially of a sequence having at least 70% sequence identity to SEQ ID NO: 11; or (iii) a polypeptide encoded by the polynucleotide shown in (i) or (ii); or (iv) an NtNTP2-S polypeptide having at least 77% sequence identity to SEQ ID NO: 7; or (v) an NtNTP2-T polypeptide having at least 77% sequence identity to SEQ ID NO: 12; wherein the expression or activity of NtNTP2-T or the expression or activity of NtNTP2-T and NtNTP2-S is reduced or inhibited as compared to a control plant.
Owner:PHILIP MORRIS PRODUCTS SA

A strain of Pteris grisea F27, a fungal agent and its application in agriculture

ActiveCN115873721BEcologically safelong-lastingBiocideFungiBiotechnologyMicroorganism
The present invention belongs to the technical field of microbial agents, and specifically relates to a strain of Emericellopsispallida F27, a microbial agent and its application in the field of agriculture. The present invention provides a strain of Emericellopsispallida F27, with a deposit number of CGMCC No. 40123, and a microbial agent is prepared using Emericellopsispallida F27. The application results show that the strain of Emericellopsispallida F27 can increase the degradation rate of corn stalks by 30.55%, and the reducing sugar concentration in the culture solution reaches 25.3 mg / L. Emericellopsispallida F27 can significantly increase the proportion of large aggregates in the soil and significantly inhibit the growth of various sickle fungi. The application of the Emericellopsispallida F27 microbial agent can promote the growth of corn and wheat, improve the stress resistance of corn, and increase soil microbial biomass carbon, microbial nitrogen, soil soluble nitrogen, soil oxygen content and straw degradation rate.
Owner:HENAN AGRICULTURAL UNIVERSITY

Burkholderia gladioli HM-72 and application thereof

ActiveCN119592462AImprove mobilityPromote absorption and accumulationBiocideAgriculture tools and machinesLead pollutionEnvironmental geology
The invention discloses Burkholderia gladioli HM-72 and application thereof, the Burkholderia gladioli HM-72 is preserved in China Center for Type Culture Collection on July 13, 2023, the preservation address is Wuhan University, Wuhan, No. 299, eight road, Wuchang District, Wuhan, Hubei, and the preservation number is CCTCC NO: M20231291. The strain is high in Pb stress resistance, and has the capability of partially dissolving phosphorus and producing siderophores. After the strain is applied to the Pb-polluted soil, the migration rate of Pb can be improved, the removal of Pb in the soil is accelerated, the Pb is accumulated in the overground part of the enrichment plant, the problem of repairing the lead pollution of the soil is solved, meanwhile, the strain can inhibit the growth of pathogenic fungi, the growth promoting and bacteriostasis characteristics of the strain can provide favorable conditions for the growth of the enrichment plant in a high-lead environment, and the application prospect is wide. The biomass accumulation is promoted, and the method has an application prospect in heavy metal polluted environment restoration.
Owner:SICHUAN AGRI UNIV

Double-gene recombinant vector for arsenic hyperaccumulation and application of double-gene recombinant vector in repairing arsenic polluted environment

The invention provides an arsenic hyperaccumulation double-gene recombinant vector and application of the arsenic hyperaccumulation double-gene recombinant vector in repairing an arsenic polluted environment, and belongs to the technical field of gene engineering. The arsenic hyperaccumulation double-gene recombinant vector takes a plant expression vector containing a pUbi promoter as a skeleton vector, and further comprises a PvACR3 gene and PvACR3, 1 gene and a prbcS promoter; wherein the pUbi promoter is used for promoting the expression of the PvACR3 gene; a prbcS promoter is used for starting the PvACR3; 1 gene expression. Aiming at the problem of rice death caused by high expression quantity of arsenic accumulation genes in transgenic rice in the prior art, through reasonable design of insertion genes and combination of promoters, toxicity of arsenic accumulation to the transgenic rice is avoided, and the arsenic removal quantity of the transgenic rice is greatly improved.
Owner:INST OF SOIL SCI CHINESE ACAD OF SCI

Bacillus orientalis YG1 with growth promoting effect on leucaena leucocephala and lespedeza bicolor and application of bacillus orientalis YG1

The invention discloses bacillus toonensis YG1 with a growth promoting effect on leucaena leucocephala and lespedeza bicolor, and belongs to the technical field of microorganisms. The bacillus toonensis YG1 has the growth promoting effect on leucaena leucocephala and lespedeza bicolor. The bacillus orientalis YG1 provided by the invention improves the conversion from total-effect nutrients to effective nutrients, and enhances the consumption of leucaena leucocephala and lespedeza bicolor on soil nutrients, thereby achieving the effect of promoting the growth of leucaena leucocephala and lespedeza bicolor. The bacillus orientalis YG1 is applied, the biomass and available phosphorus of lespedeza leucocephala are increased by 181.73% and 50.32% respectively most obviously, and the content of hydrolytic nitrogen and the content of organic matter of lespedeza are increased by 45.18% and 48.90% respectively most obviously. Other physicochemical indexes in leucaena leucocephala soil and lespedeza soil are obviously increased, and meanwhile, the soil enzyme activities of the leucaena leucocephala soil and the lespedeza soil are In general, the improvement of soil physicochemical properties and the significant increase of soil enzyme activity by inoculating the YG1 microbial inoculum may be an important factor for promoting plant growth.
Owner:CHINA CONSTR FIRST BUILDING (GRP) CORP LTD +2

Method for synthesizing astaxanthin through fermentation of phaffia rhodozyma

The invention relates to the technical field of microbial fermentation, in particular to a method for synthesizing astaxanthin through fermentation of phaffia rhodozyma. According to different optimal conditions of phaffia rhodozyma growth culture conditions and astaxanthin synthesis conditions, a variable-temperature and batch-feed culture strategy is adopted, and a composite deep eutectic solvent is added into a feed culture medium, so that the astaxanthin yield of the phaffia rhodozyma is improved due to the action of the deep eutectic solution in the astaxanthin production stage. On one hand, the permeability of microbial cell membranes is increased, extracellular substrates are promoted to be conveyed into cells to be fermented, metabolized and utilized, on the other hand, the deep eutectic solvent can further enter the cells to adjust the activity of astaxanthin metabolism synthesis key enzymes and cause intracellular acid-base imbalance, metabolic flux of phaffia rhodozyma for synthesizing astaxanthin is promoted to be enhanced, and the astaxanthin metabolism effect is improved. Therefore, the biomass of phaffia rhodozyma and the intracellular astaxanthin yield of phaffia rhodozyma are effectively increased.
Owner:NANCHANG UNIV

Ionic rare earth ore tailing sandy land soil improvement structure and improvement method

PendingCN119586518Alow costRestoring ecological functionsGrowth substratesCulture mediaSoil sciencePoultry manure
The invention relates to an ion type rare earth ore tailing sandy land soil improvement structure and an improvement method, and belongs to the technical field of ecological restoration. The ion type rare earth ore tailing sandy land soil improvement structure sequentially comprises a humus layer, a surface soil layer and a subsoil layer. The subsoil layer comprises rare earth ore tailing sandy land soil and a slow-release modifier; the surface soil layer comprises the rare earth ore tailing sandy land soil, a slow-release modifier and a quick-acting modifier; the slow-release improver comprises agricultural and forestry wastes, and the quick-acting improver comprises livestock and poultry manure, lime and a compound fertilizer. According to ecological function characteristics of the soil, aiming at physical characteristics and nutrient content of the soil and specific rare earth and aluminum toxicity pollution of a mining area, the soil layer is remolded by using different dosages and different types of improvers, so that the restoration of a local damaged soil ecological system is accelerated, and the ecological function restoration of the soil in the rare earth ore tailing sand land is realized.
Owner:SUN YAT SEN UNIV

Application of new mycobacterium spp. Strain and sudangrass in combined remediation of uranium-contaminated soil

The invention discloses an application of a novel mycobacterium spp. Strain and sudangrass in combined remediation of uranium-contaminated soil. According to the invention, a new strain Microvirga sp.M2 is separated from a sandy soil sample of a tailing dam in the southwest region, and the bacterial colony of the new strain Microvirga sp.M2 is light pink, round, glossy in surface, convex in middle, smooth and tidy in edge, thin in texture and easy to emulsify on NA; the cells are rod-shaped, the ends of the cells are round, and the cells are independent, paired or short-chain-cluster-shaped, have no flagellum and do not generate spores. The micromycobacterium sp.M2 strain not only has the characteristics of nitrogen fixation, phosphorus solubilization, siderophore synthesis and IAA (Indoleacetic Acid) production, but also has good tolerance to uranium; meanwhile, the strain also has good growth promoting ability, has a better protective effect on relieving the toxicity of uranium to seed germination stress, can relieve the toxicity of uranium to plant growth, and can significantly increase the biomass of plants and the enrichment amount of uranium.
Owner:THE INST OF MICROBIOLOGY XINJIANG ACADEMY OF AGRI SCI

Method for promoting growth or resisting diseases of oilseed rapes

The invention discloses a method for promoting growth or resisting diseases of oilseed rape. The research finds that any one or a combination of more of bacillus subtilis B.subtilis 168, trichoderma harzianum T.harzianum 25852 and trichoderma harzianum T.harzianum 52623 can effectively promote the growth of rape seedlings, and the growth mainly shows that the plant stem diameter, the plant height and the increase of the biomass of the overground part and the underground part. Wherein the trichoderma harzianum T.harzianum 25852, and the combination of the bacillus subtilis B.subtillis 168 and the trichoderma harzianum T.harzianum 25852 also have a relatively good sclerotinia rot prevention and treatment effect. The microorganism provided by the invention has the characteristics of high efficiency, no pollution, difficult generation of drug resistance and the like in the aspect of oilseed rape production, has great application potential, and has important significance for promoting the improvement of oil productivity and green low-carbon efficient production in China.
Owner:OIL CROPS RES INST CHINESE ACAD OF AGRI SCI

Comprehensive prevention and control method for leaf mites of under-forest polygonatum sibiricum

The invention relates to the technical field of traditional Chinese medicinal material production, in particular to a green prevention and control method for main leaf pests of tetranychus urticae and tetranychus crataegi in the under-forest ecological planting process of polygonatum sibiricum, and the method comprises the steps of S1, egg overwintering prevention and control, S2, non-host plant repelling, S3, biological agent killing, S4, agronomic measure blocking, and S5, late autumn tannin insect prevention and egg inhibition. Aiming at the technical problem of prevention and control of leaf mites of polygonatum sibiricum, from the perspective of sustainable development of traditional Chinese medicine ecological agriculture, an ecological technology is used in comprehensive treatment of leaf pests of polygonatum sibiricum, a green prevention and control implementation path for tetranychus urticae and tetranychus crataegus is provided, pollution of agricultural chemicals can be reduced, and the environment-friendly prevention and control of leaf mites of polygonatum sibiricum is realized. Meanwhile, green forest ecological traditional Chinese medicine products are produced, the rhizome yield per unit area is increased by 9.26%, it is ensured that the first-grade product rate is higher than that of conventional cultivation by 13.58% or above, and economic benefits are improved.
Owner:BEIJING FORESTRY UNIVERSITY

Slow-release bacterial fertilizer, preparation method and application

The invention discloses a slow-release bacterial fertilizer as well as a preparation method and application thereof, and relates to the technical field of biotechnology. Comprising the following steps: step 1, preparing a bacterial liquid of bacillus cereus, a bacterial liquid of lysinibacillus slender, a bacterial liquid of acinetobacter schiffei, a bacterial liquid of stenotrophomonas maltophilia, a bacterial liquid of sphingobacterium alcohovorans and a bacterial liquid of bacillus licheniformis into a mixed bacterial suspension according to a volume ratio of 1: (1-2): (1-2): (1-2): (1-2): (1-2); 2, mixing the mixed bacterium suspension with an embedding agent and a cross-linking agent to obtain a first mixed colloid; step 3, embedding the first mixed colloid into the second mixed layer to obtain first compound fertilizer particles; and step 4, embedding the first compound fertilizer particles into a fermented third mixed layer to obtain the slow-release bacterial fertilizer. The prepared slow-release bacterial fertilizer improves the remediation effect of organic phosphorus contaminated soil. The soil fertility is improved, and a good growth promoting effect on plants is achieved.
Owner:ANQING NORMAL UNIV

High-concentration ammonia nitrogen rural domestic sewage treatment system

The invention relates to the technical field of sewage treatment, and provides a high-concentration ammonia nitrogen rural domestic sewage treatment system, which comprises an adjusting tank, a sewage basic treatment device, a sewage enhanced treatment device, an aeration device and a bacteria inactivation device, the sewage basic treatment device comprises an anaerobic tank, an anoxic tank, an aerobic tank and a sedimentation tank which are sequentially communicated, a first sludge return pipe is communicated between the anaerobic tank and the sedimentation tank, and a second sludge return pipe is communicated between the aerobic tank and the anoxic tank; the sewage enhanced treatment equipment is communicated between the sewage basic treatment equipment and the adjusting tank, the sewage enhanced treatment equipment comprises a biochemical tank, and an aerobic tank and an anoxic tank which are arranged in the biochemical tank, and the aerobic tank and the anoxic tank are communicated with each other; the aeration equipment is used for aerating the anoxic tank, the aerobic tank, the anoxic tank and the aerobic tank; the bacteria inactivation equipment is used for inactivating nitrifying bacteria in the second sludge return pipe. According to the invention, ammonia nitrogen in sewage can be efficiently removed, and the denitrification efficiency is improved.
Owner:GUANGDONG GUANGYE ENVIRONMENTAL PROTECTION SERVICE CO LTD

In-situ remediation method and system for heavy metal contaminated soil

The invention discloses an in-situ remediation method and system for heavy metal contaminated soil. The method comprises the steps that a remediation area is established in situ along the heavy metal contaminated soil, an external electric field is applied to the two ends of the remediation area, enrichment plants are evenly planted in the remediation area, the external electric field is communicated with a direct-current power source, and the voltage gradient of the remediation area is controlled to be 0.3 V / cm to 0.5 V / cm. The heavy metal contaminated soil is remedied through the enrichment plants, meanwhile, the anode electrolysis chamber and the cathode electrolysis chamber are used for applying an external electric field to the remediation area, the remediation efficiency is greatly improved through cooperation with the enrichment plants, and the purpose of cleaning the contaminated soil is achieved.
Owner:CHINA MCC5 GROUP CORP LTD

Method for dynamically regulating alcohol content to enhance the flavor of solid aromatic vinegar and preparation of aromatic vinegar

PendingCN122503196APrecisely control the fermentation processimprove integrity
The application provides a method for dynamically regulating alcohol content to enhance the flavor of solid vinegar, comprising the following steps: S1, liquefaction; S2, saccharification; S3, alcohol fermentation; S4, solid-state layered fermentation; and S5, finished product preparation. The vinegar preparation method has the advantages that the fermentation performance is improved, the fermentation period is shortened, the conversion rate of acetic acid is improved, the biomass of bacteria is increased, the growth of acetic acid bacteria is as good as possible, the rate of acetic acid fermentation is improved, the period of acetic acid fermentation is shortened, the flavor of liquid-state fermented vinegar is improved, the irritation of solid-state fermented vinegar is reduced, and the method has a good market application prospect.
Owner:JIANGSU HENGSHUN VINEGAR IND +1

Method for improving soil chromium pollution by using irrigation and fertilization period and dosage combination

The invention discloses a method for optimizing and improving the productivity of chromium-contaminated soil by using irrigation and fertilization period and dosage combination, and the steps are: selecting a crop variety of silage corn, sowing according to the dense planting sowing amount, randomly sowing and synchronously applying compound fertilizer and humic acid fertilizer as base fertilizer, irrigating water during the sowing period; irrigating again during the jointing period or the large trumpet period of the crop, and simultaneously flushing and applying humic acid fertilizer for combined regulation; irrigating again during the heading and filling period of the crop. The method of the present invention applies humic acid fertilizer during the sowing period, jointing period or the large trumpet period, uses river water for irrigation during the sowing period, jointing period or the large trumpet period, and uses well water for irrigation during the heading and filling period to promote a significant increase in corn biomass, reduce the chromium content of the crop, and reduce the residual amount of total chromium and effective chromium in the root layer soil at the same time, and uses well water for irrigation during the heading and filling period to effectively block the transportation of effective chromium in the soil to the crop plant body, thereby achieving the improvement of chromium-contaminated farmland soil productivity and green restoration of soil chromium pollution.
Owner:FARMLAND IRRIGATION RES INST CHINESE ACAD OF AGRI SCI

Stable efficient doubling and rapid propagation integrated method for sesbania cannabina

The invention provides a sesbania cannabina doubling and rapid propagation integrated method, and relates to the technical field of sesbania cannabina breeding, and the method comprises the following steps: rubbing sesbania cannabina seeds to break seed coats, disinfecting the seeds, soaking the sesbania cannabina seeds for hydration, inducing genome doubling by colchicine, and then carrying out rooting culture; after cotyledons are unfolded, water culture seedling hardening is carried out; after compound leaves grow, genome ploidy identification is carried out, and the successfully doubled sesbania can be transplanted. The period of the whole method is 18 days, and the sesbania polyploidy induction rate can reach 50% or above. According to the method, sesbania genome doubling is achieved for the first time, a large number of polyploid plants meeting the breeding requirement can be directly obtained, the seedling raising time can be shortened, the plant survival rate can be increased, the production cost can be reduced, and integration of sesbania genome doubling and plant rapid propagation is achieved.
Owner:INST OF GENETICS & DEVELOPMENTAL BIOLOGY CHINESE ACAD OF SCI

Application of OsMYBR17, a MYB transcription factor that promotes both tillering and panicle development, in improving rice yield

The present invention belongs to the technical field of plant genetic engineering, and relates to the technical field of crop breeding, and specifically relates to an application of a MYB transcription factor OsMYBR17 gene that simultaneously promotes tillering and panicle development in increasing rice yield, wherein the protein encoded by the OsMYBR17 gene is a protein with an amino acid sequence as shown in SEQ ID NO.1; or it is a protein with equivalent activity obtained by replacing, substituting and / or adding one or more amino acids to the amino acid sequence as shown in SEQ ID NO.1. This technical solution has discovered for the first time a gene that simultaneously positively regulates tillering and panicle development, which can promote rice tillering, increase the number of rice tillers, the panicle length of a single panicle branch, the weight of a single panicle, and the number of panicle grains per panicle, thereby increasing rice yield. The overexpression of this gene can be applied to the genetic improvement of rice plant type and yield, creating conditions for increasing rice yield and cultivating rice with a new plant type.
Owner:GUIZHOU UNIV

KYJ protein for negative regulation of saline-alkali resistance of poplar and coding gene and application of KYJ protein

The invention discloses two KYJ proteins for negative regulation of saline-alkali resistance of poplar and coding genes and application thereof, the amino acid sequence of the KYJ proteins is shown as SEQ ID NO.2 or SEQ ID NO.5, and the sequence of the coding gene kyj is shown as SEQ ID NO.1 or SEQ ID NO.4. The invention further discloses a preparation method of the KYJ proteins. Experiments prove that under the saline-alkali condition, compared with a wild type, the kyj gene double-process strain is higher in plant height, stronger in photosynthesis, larger in biomass and better in growth vigor, that is, the saline-alkali resistance of the kyj gene double-process strain is obviously superior to that of the wild type material, which indicates that the kyj gene plays an important regulation function in the process that the poplar responds to saline-alkali stress; the method has important guiding significance for genetic improvement of the poplar.
Owner:BEIJING FORESTRY UNIVERSITY

Composition for repairing heavy metal polluted alkaline soil and application thereof, and method for repairing heavy metal polluted alkaline soil

The present invention belongs to the technical field of contaminated soil treatment, and specifically relates to a composition for repairing heavy metal-contaminated alkaline soil, its application, and a method for repairing heavy metal-contaminated alkaline soil. Hydroxyapatite and humic acid in the present invention can reduce active heavy metals in alkaline soil, reduce the toxic effects of heavy metals on giant fungus grass, and increase the biomass of giant fungus grass. Hydroxyapatite combined with humic acid and giant fungus grass treatment has no obvious effect on the pH value and conductivity of alkaline soil, while increasing the organic matter in alkaline soil and reducing the available phosphorus, which is beneficial to improving soil fertility. There is no risk of secondary pollution caused by exogenous phosphorus, showing a certain degree of environmental friendliness. The presence of giant fungus grass can also increase the number of alkaline soil microorganisms, enrich the diversity of soil microbial communities, and promote the improvement of the ecological environment of alkaline soil.
Owner:LANZHOU UNIV

Trichoderma sp. Y3 and application thereof in promoting rice growth

The invention discloses trichoderma sp. Y3 and application thereof in promoting rice growth, and belongs to the technical field of microorganisms. The classification name of the trichoderma sp. Y3 is Trichoderma sp. Y3, the trichoderma sp. Y3 is preserved in the China Center for Type Culture Collection, and the preservation number is CCTCC NO: M 2026214. And biological characteristics and molecular identification are combined to identify that the trichoderma Y3 belongs to a new trichoderma species. When the strain or a fermentation product thereof is applied to a rice culture medium, the growth of rice can be effectively promoted, and the plant biomass is increased. Trichoderma Y3 is developed and prepared into the biological bacterial fertilizer, and a new way is provided for application of microorganisms to development of crop production.
Owner:ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES

Method for culturing industrial cannabis sativa adventitious roots with high yield of cannabinoid and tanshinone

The invention relates to a method for culturing industrial cannabinoid and tanshinone high-yield industrial cannabinoid adventitious roots, and belongs to the technical field of adventitious root cultivation. The method comprises the following steps: performing induction treatment on the industrial cannabis sativa adventitious roots by adopting a yeast extract to obtain the industrial cannabis sativa adventitious roots subjected to induction treatment; the yeast extract is low in cost and abundant in raw material supply, and can stimulate industrial cannabis sativa adventitious roots to synthesize more target metabolites as an elicitor; the industrial cannabis sativa adventitious roots subjected to induction treatment are subjected to scale-up culture by utilizing a bioreactor, so that cell proliferation and growth of the adventitious roots are promoted, and the biomass of the adventitious roots is increased; meanwhile, the adventitious roots are promoted to accumulate more target metabolites, and at the moment, the yields of cannabinoid phenol, cannabidiol, tetrahydrotanshinone I, tanshinone IIA and dihydrotanshinone I in the adventitious roots are up to 80.65 mg / L DW, 53.58 mg / L DW, 103.76 mg / L DW, 21.71 mg / L DW and 5.71 mg / L DW respectively. According to the method, the operation steps and the labor intensity are reduced, the defects of low biomass and low cannabinoid yield in the existing industrial cannabinoid adventitious root culture technology are overcome, and the industrial cannabinoid and tanshinone high-yield industrial cannabinoid adventitious roots can be economically and efficiently cultured all the year round.
Owner:NORTHEAST FORESTRY UNIV +1

An engineered strain for producing L-isoleucine, a construction method and application thereof

This invention relates to the fields of transcription factor engineering and metabolic engineering, and discloses an engineered strain that produces L-isoleucine, its construction method, and its applications. This strain... E. coli K12, MG1655 ( E. coli A1) serves as the host, integrating the subunit of the host factor IHF. ihfβ, ihfα Natural promoter replaced with P trc Strong promoters enable IHF overexpression, resulting in engineered bacteria. E. coli IHF4. This invention also discloses the construction steps of this strain, as well as its application method for L-isoleucine production by inoculating it into a fermentation medium, culturing it at 36-38℃ and 200 r / min for 40-60 h, and adjusting the pH and supplementing sugar. This engineered strain increases the yield of L-isoleucine by 42.11% compared to the starting strain. The construction method is simple and suitable for industrial microbial fermentation production of L-isoleucine.
Owner:NINGXIA UNIVERSITY

Drought-resistant growth-promoting bacterium, fungicide, application and plant drought-resistant growth-promoting method

The invention relates to the field of microorganisms, in particular to a drought-resistant growth-promoting bacterium, a fungicide, application and a plant drought-resistant growth-promoting method. The strain is Rhodobacter sp. K28, is preserved in the China Center for Type Culture Collection on January 29, 2024, and has the preservation number of CCTCC (China Center for Type Culture Collection) NO: M 2024252. The strain can enhance the growth of arabidopsis thaliana under drought stress and promote the increase of the amount of dry matter of arabidopsis thaliana. The method is used for improving the drought resistance of arabidopsis thaliana and promoting the growth of arabidopsis thaliana.
Owner:NORTHEAST INST OF GEOGRAPHY & AGRIECOLOGY C A S

Modified small nuclear RNA (Ribonucleic Acid) and application of protein complex of modified small nuclear RNA in enhancing photosynthesis of plants

The invention discloses application of modified small nuclear RNA (Ribonucleic Acid) and a protein complex thereof in enhancing plant photosynthesis. The invention relates to the technical field of biology, and provides a small nuclear RNA (Ribonucleic Acid) of which the nucleotide sequence is SEQ ID NO: 1 or SEQ ID NO: 2. The invention also provides a small nuclear RNA-protein complex composed of the small nuclear RNA and an Sm protein specifically bound with the small nuclear RNA. The small nuclear RNA and the protein complex thereof disclosed by the invention can be specifically combined with mRNA 3 'UTR regions of a plurality of nuclear coding chloroplast protein genes through base complementary pairing, and the expression of the nuclear coding chloroplast protein is enhanced on the translation level, so that the biosynthesis of chloroplast is promoted, and the photosynthetic efficiency of plants is improved.
Owner:PEKING UNIV

Wickerhamomyces anomalus WAS1 and application thereof

The invention relates to the technical field of biology, in particular to a strain Wickerhamomyces anomalus WAS1 and application of the strain Wickerhamomyces anomalus WAS1. The preservation number of the strain WAS1 is CDMCC (China General Microbiological Culture Collection Center) NO Experiments show that the strain can be applied to preparation of organic fertilizers, can promote crop growth and improve crop yield and quality, has a remarkable effect, and provides an excellent strain resource for the fields of crop yield increase application and the like.
Owner:GUANGXI ZHUANG AUTONOMOUS REGION ACAD OF AGRI SCI

Nucleotide molecule for silencing tobacco squalene epoxidase gene NtSQE1 and application thereof

The invention belongs to the field of plant genetic engineering, and provides a nucleotide molecule, a recombinant vector and a recombinant host cell for silencing a squalene epoxidase gene NtSQE1 in tobacco and application of the nucleotide molecule, the recombinant vector and the recombinant host cell in increasing the squalene content in the tobacco, and the SEQ ID NO: 2 sequence in the squalene epoxidase gene NtSQE1 is inhibited in a targeted mode through a specific virus-induced gene silencing technology (VIGS). The accumulation level of squalene in a plant body can be remarkably improved under the condition that the plant phenotype is not influenced, and the silencing efficiency is high.
Owner:BEIJING LIFE SCIENCE ACADEMY CO LTD

System and method for synthesizing biodegradable plastic raw material by recycling sludge

The invention discloses a system and a method for synthesizing a biodegradable plastic raw material through sludge resource utilization, and relates to the field of sludge resource utilization. The system comprises a sludge anaerobic acid production unit, a filtering unit, a feeding unit, a phosphorus recovery unit, a nutrient element supplementing unit, a strain domestication and propagation tank, a PHA accumulation synthesis tank, an aeration unit and an online monitoring and automatic control unit. The method comprises the following steps: adding residual sludge and digested sludge into a sludge anaerobic acidogenic unit, conveying discharged acidogenic sludge to a filtering unit, filtering to obtain acidogenic filtrate, and discharging the acidogenic filtrate into a feeding unit; adding the activated sludge into a strain domestication and propagation tank, and regulating and controlling the adding time and the adding amount of the acid-producing filtrate; after flora in the strain domestication and propagation tank is stable, sludge in the strain domestication and propagation tank is discharged into a PHA accumulation synthesis tank, and when the PHA synthesis rate reaches the highest, bacterial liquid is discharged, and a PHA crude product is collected. Therefore, the PHA synthesis rate and the PHA synthetic bacterium biomass can be improved at the same time, and the contradiction between PHA synthetic bacterium biomass amplification and synthesis rate improvement is solved.
Owner:BEIJING DRAINAGE GRP CO LTD

Aerobic biological fluidized bed sewage treatment equipment

The utility model relates to sewage treatment equipment of an aerobic biological fluidized bed, which belongs to the technical field of sewage treatment and comprises the aerobic biological fluidized bed which is of a hollow tank body or cylindrical structure and is internally provided with a hydrophilic microbial carrier; a sieve tray is arranged at the bottom of the aerobic biological fluidized bed, and a flow stabilizing cylinder is arranged at the upper part of the aerobic biological fluidized bed; the flow stabilizing cylinder comprises a straight cylinder section and a big-end-down horn cylinder connected to the lower portion of the straight cylinder section. A settling chamber is formed by the guide cylinder and the outer wall of the horn-shaped cylinder; a sludge return channel is reserved between the bottommost end of the horn and the bed wall of the aerobic biological fluidized bed, and the size of the sludge return channel is smaller than the diameter of the hydrophilic microbial carrier; a gap is reserved between the guide cylinder and the steady flow cylinder, and an interception net is arranged in the gap; the reactor disclosed by the utility model has the characteristics of high volume load, small occupied area, large biomass, impact load resistance and no loss of filler carriers and microorganisms.
Owner:YUNNAN ZHIDE ENVIRONMENTAL PROTECTION TECH CO LTD