1210results about How to "Large biomass" patented technology

The active sludge-biomembrane compounding integral sewage treating method includes the first leading the sewage to be treated to the anoxic area through stirring for mixing sewage to be treated and active sludge, the subsequent leading the sewage to aerobic area with stuffing for aeration treatment with combined active sludge and biomembrane, and final leading the sewage to the precipitation area for natural deposition to separate sludge and water, leading out purified water, reflowing the mixed sludge liquid to the anoxic area and draining the residual sludge. The present invention also relates to the corresponding sewage treating apparatus. The present invention has the advantages of high treating capacity, resistance to impact load, simple operation, etc. and is especially suitable for use in medium and small towns.

An anti-impact multiplying combination coking wastewater treatment technique relates to a treatment method of coking wastewater. The coking wastewater is first qualified by a physical-chemical pretreatment to improve biodegradable property of wastewater; the composite technology of a biological carrier of active sludge is adopted to do anaerobic/hypoxia/aerobic decarbonization/aerobic nitrification biochemical combination treatment; suspended or floating folding ball-shaped fillings are arranged in biochemical reactors and when micro organism traits are abnormal or a great amount of foams existing in the reactor, micro organism active incentive agents are added to the reactor in interval to improve active sludge traits and organism film quantity; finally the water is treated by a physical-chemical advanced treatment to reach a top grade standard. The invention not only strengthens the removal of COD, ammonia-nitrogen, volatile phenol and other pollutants in the coking wastewater by biochemical treatment but also resists serious water quality impact and has the advantages of quick system startup, less foams in an aeration tank, small occupying space, low operation cost and stable and effective treatment to coking wastewater.

Transgenic chloroplast technology could provide a viable solution to the production of Insulin-like Growth Factor I (IGF-I), Human Serum Albumin (HSA), or interferons (IFN) because of hyper-expression capabilities, ability to fold and process eukaryotic proteins with disulfide bridges (thereby eliminating the need for expensive post-purification processing). Tobacco is an ideal choice because of its large biomass, ease of scale-up (million seeds per plant), genetic manipulation and impending need to explore alternate uses for this hazardous crop. Therefore, all three human proteins will be expressed as follows: a) Develop recombinant DNA vectors for enhanced expression via tobacco chloroplast genomes b) generate transgenic plants c) characterize transgenic expression of proteins or fusion proteins using molecular and biochemical methods d) large scale purification of therapeutic proteins from transgenic tobacco and comparison of current purification/processing methods in E. coli or yeast e) Characterization and comparison of therapeutic proteins (yield, purity, functionality) produced in yeast or E. coli with transgenic tobacco f) animal testing and pre-clinical trials for effectiveness of the therapeutic proteins. Mass production of affordable vaccines can be achieved by genetically engineering plants to produce recombinant proteins that are candidate vaccine antigens. The B subunits of Enteroxigenic E. coli (LTB) and cholera toxin of Vibrio cholerae (CTB) are examples of such antigens. When the native LTB gene was expressed via the tobacco nuclear genome, LTB accumulated at levels less than 0.01% of the total soluble leaf protein. Production of effective levels of LTB in plants, required extensive codon modification. Amplification of an unmodified CTB coding sequence in chloroplasts, up to 10,000 copies per cell, resulted in the accumulation of up to 4.1% of total soluble tobacco leaf protein as oligomers (about 410 fold higher expression levels than that of the unmodified LTB gene). PCR and Southern blot analyses confirmed stable integration of the CTB gene into the chloroplast genome. Western blot analysis showed that chloroplast synthesized CTB assembled into oligomers and was antigenically identical to purified native CTB. Also, GM1,-ganglioside binding assays confirmed that chloroplast synthesized CTB binds to the intestinal membrane receptor of cholera toxin, indicating correct folding and disulfide bond formation within the chloroplast. In contrast to stunted nuclear transgenic plants, chloroplast transgenic plants were morphologically indistinguishable from untransformed plants, when CTB was constitutively expressed. The introduced gene was stably inherited in the subsequent generation as confirmed by PCR and Southern blot analyses. Incrased production of an efficient transmucosal carrier molecule and delivery system, like CTB, in transgenic chloroplasts makes plant based oral vaccines and fusion proteins with CTB needing oral administration a much more practical approach.

The invention relates to an energy source plant-mushroom fungus combined renovating technique for heavy metal polluted soil, belonging to the technical field of environmental energy sources. Energy source plants and mushroom fungus are planted in intercrop, interplanting or crop rotation on the polluted soil for enriching heavy metal ions; after materializing processing or biofermentation, the mature plants generate energy source substance to be used in the field of industrial fuel; plant residue and mushroom fungus body are put in a biogas tank with certain proportion to generate biogas; heavy metal ions in the methane liquid can be removed through chemical deposition; and the chemical deposition and biogas residues are intensively filled and buried. The energy source plant enricher in the invention can renovate deep soil, beautify the environment and generate energy source benefits and can be planted in large area, while the mushroom fungus enricher has the advantages of strong heavy metal ion enriching ability, short growth period and mature planting technique; the energy source plant and the mushroom fungus are combined to renovate the heavy metal polluted soil so as to achieve higher renovating efficiency and get energy benefits. 10-20 percent of polluted heavy metal can be removed in the cultivation layer of the soil per year.

The invention discloses a method for promoting plant growth by illumination, in particular to designing a light source product with universal meaning according to the spectrum demanded by plant growth, therefore the method of supplementary illumination of plants in raising seedling of plastic tents and factories is found. The method comprises the following processes: (1) seedling preparation; (2) confirming the absorption spectrum of the plants; (3) testing the photosynthesis under different light qualities; (4) comparing and analyzing the experiments so as to confirm the light absorption of different plants; (5) designing light source equipment; (6) supplementary illumination is carried out to the plants. In the method, spectrum test equipment is used for testing the plants with different types; the light sources with different wavelengths are chosen and made according to the absorption spectrum of the plants, therefore the pertinence and efficiency of the light source can be improved efficiently. The bud ratio and the survival ratio can also be improved efficiently, the seedling state can be shortened, the biomass of seedlings can be increased, and the height of the seedlings and the number of leaves are promoted for increasing, thus shortening the growth period of the plants efficiently and improving the efficiency and benefit during raising seedling and growth.

The invention discloses an integrated type ecological floating bed and the water body ecological system repairing technology thereof. A physical space of the integrated type ecological floating bed is formed by constructing a floating bed, a sinking bed, a buoyancy adjusting bottle, a lifting chain and an ecological barricading net, and integrates ecological components such as indigenous emergent aquatic plants, submerged plants, attached aquatic plants, fishes, snails and biochemical zeolite. The ecological behaviors of the ecological components are in linear cause and effect relation. The ecological components and organisms in the physical space form a semi-enclosed ecological subsystem with integral completeness. The upper line and the water body ecological system to be repaired are strong in coupling capability, and ecological factors in the lower line can exist stably. The damaged water body ecological system sufferring from one or a plurality of types of pollutions including water body eutrophication, heavy metal pollution, organic pollution and the like can be repaired by building pioneer communities and utilizing self-cleaning capability of the ecological system, pollution is removed, integral restoration or rebuilding of the damaged water body ecological system is promoted, simultaneously environment is beautified, and ecological deterioration of a healthy water body can be prevented.

The invention belongs to the ferment industry, in particular relates to a treatment method of ion exchange waste liquor in lysine production by a fermentation method. The invention adopts a common electrodialysis technology to recycle ammonium sulfate, ammonium chloride or ammonium nitrate in the lysine ion exchange waste liquor produced by extracting the lysine from lysine fermentation production to water solution of ammonium sulfate, ammonium chloride or ammonium nitrate, or adopts a bipolar membrane electrodialysis technology to regenerate sulfuric acid, hydrochloric acid or nitric acid and NH3 by ammonium sulfate, ammonium chloride or ammonium nitrate in the lysine ion exchange waste liquor produced by extracting the lysine from the lysine fermentation production, or adopts the combination of the common electrodialysis technology and the bipolar membrane electrodialysis technology to recycle ammonium sulfate, ammonium chloride or ammonium nitrate in the lysine ion exchange waste liquor produced by extracting the lysine from the lysine fermentation production and regenerate sulfuric acid, hydrochloric acid or nitric acid and NH3. The invention realizes the resource recycling of the ion exchange waste liquor and clean production.

The invention discloses a fungus strain, which is Phialophora oryzae R5-6-1 fungus strain with the preservation organization of China General Microbiological Culture Center of Microbial Culture Collection Management Committee, the preservation date of October 27th, 2008 and the preservation number of CGMCC 2737. The fungus strain can be used for improving the growth of vegetables especially paddy rice and increasing the live weight thereof.

The invention provides an application of arbuscular mycorrhizal fungi (AMF) in enhancement of growth and capability of heavy metal absorption from soil of black nightshade and a method of enhancing cadmium absorption of black nightshade from soil by utilization of the arbuscular mycorrhizal fungi. A cadmium (Cd) hyperaccumulating plant black nightshade is adopted as an experiment plant, the AMF is adopted as an Inoculant, the Cd hyperaccumulating plant black nightshade and the AMF capable of promoting plant growth are combined, and the AMF can enhance growth and capability of cadmium absorption from soil of the black nightshade, thus increasing the remediation efficiency of the black nightshade for Cd-contaminated soil. Compared with traditional chemical and agricultural measures, the method has characteristics of good treatment effects, no secondary pollution, low operation cost, and the like, and has good theoretical and application popularization value.

The invention provides a method for restoring uranium milltailings by utilizing plants, which is a technique for restoring the uranium milltailings by utilizing uranium-enriched plants (macleaya cordata) as the material. The method directly plants macleaya cordata into the uranium milltailings without other supplementary means and comprises the following steps: (1) sterilizing seeds; (2) plantingseedlings in pots; (3) transplanting the plants into the uranium milltailings; (4) and carrying out the centralized processing on the reaped overground part. The method solves the problem that the prior uranium milltailings release uranium and heavy metals under natural conditions to damage the environment. The method has the advantages of simple operation and management, high restoration rate aswell as low cost and environmental risk and beautifies the environment. The method is suitable for treating solid uranium waste storage yards in uranium mines, hydrometallurgy plants, various ettle and milltailing warehouses, and the like.

A phytoremediation method based on soil contaminated by uranium tailings comprises the steps for culturing pokeberry seedlings on a substrate containing uranium tailings, surviving, growing, harvesting and centrally processing. The method comprises the following steps of: (1) mixing soil contaminated by uranium tailings, surface oil of forest and pig manure according to a ratio to obtain a culture substrate; (2) adding sodium silicate in the culture substrate; (3) sterilizing seeds; and (4) potting. By adopting the comprehensive processing scheme combined with the pokeberry, the uranium tailings, the surface oil of forest, the pig manure and the sodium silicate, the method can solve the difficult problem that the environment can be endangered by heavy metal, uranium, which is released by uranium tailings under natural condition. The method has the advantages of convenient operation and management, high remedying efficiency, low cost, low environment risk, environment beautification, etc., and is suitable for processing solid-state uranium waste storage fields such as uranium mines, hydrometallurgy plants and waste ore and tailings reservoirs.

The invention relates to a method for culturing microalgae, which is characterized by comprising the following steps of: (1) fermenting to produce acid, enabling the supernatant which is acid liquor obtained after fermentation to flow to a culture device, and collecting the produced gas mixture in a gas storage tank; (2) inoculating the cultured microalgae to the acid liquor contained in the culture device for heterotrophic culture; and (3) circularly introducing the gas mixture in the gas storage tank to a photobioreactor for aerobic autotrophic culture. In the invention, the acid production stage through methane fermentation and the microalgae culturing process are coupled, and heterotrophy and autotrophy are combined; thus, oil-rich microalgae with high biomass is cultured, and meanwhile, a hydrogen-rich gas with high concentration can be obtained. The method is mainly used for culturing the oil-rich microalgae.

The invention relates to a sponge iron-AT (Aluminium Titanate)-PVF (Polyvinyl Fluoride) composite filler and application thereof on water treatment. The composite filler is formed by filling attapulgite composite hydrophilic polyurethane foam of the size of 1.5*1.5*1.5cm and sponge with the grain diameter of 2-5cm at the weight ratio of 1: (4-50). The sponge iron-AT-PVF composite filler has the characteristics of low cost, good water treatment effect, high degrading speed and high removal rate on elements N and P as well as organic matters which are difficult to be degraded in the wastewater. The TN (Total Nitrogen) and TP (Total Phosphorus) indexes in the effluent can be drastically lowered under the aerobic or anaerobic conditions when the composite filler is put into a common biochemical treating pond.

The invention relates to a biology processing apparatus capable of processing sewage at low-temperature with low carbon-nitrogen-ratio in a cold region and a method for processing sewage at low-temperature with low carbon-nitrogen-ratio in the cold region, which relates to the biology processing apparatus for processing sewage and the method for processing sewage. According to the invention, problems that the ratio of carbon source and nitrogen source of sewage in winner at the cold region is low, the synchronization nitrogen and phosphorus removal efficiency in the current sewage processing technology is low, the index of nitrogen and phosphor in a processed effluent can not reach the standard can be solved. The apparatus comprises a water storage tank, a pre-conditioning hypoxia pool, an anaerobic pond, an anoxic pool, an aerobic tank, a sedimentation basin, an aeration head, a filling material, a stirrer, a water inlet pump, a reflux pump, a valve, an outlet pipe, a sludge discharge pipe and a residual sludge discharge pipe; The method comprises the following steps: 1)culturing microbe on a filling material; 2)adjusting operation parameter; 3)adjusting reflux ratio parameter; 4)passing the sewage through the pre-conditioning hypoxia pool, the anaerobic pond, the anoxic pool, the aerobic tank and the sedimentation basin for processing to obtain the processed water. The apparatus and the method can be used for construction and reconstruction of the sewage processing technology at low-temperature with low carbon-nitrogen-ratio in a north region.

The invention belongs to the field of sewage treatment and provides a short distance denitrifying dephosphatation double-sludge technique taking granular sludge as medium and a device thereof. The device sequentially comprises a raw water pipe, a water inlet tank and a water inlet valve, and is characterized by further sequentially comprising an anaerobic/anoxic SBR reactor and an intermediate water tank which are connected with each other by a pipeline as well as a short distance nitration SBR reactor and a back flow water tank that are also connected with each other by the pipeline; wherein the intermediate water tank is connected with the short distance nitration SBR reactor, and the back flow water tank is connected with the anaerobic/anoxic SBR reactor; the anaerobic/anoxic SBR reactor and the short distance nitration SBR reactor are internally provided with stirring devices, and a drain pipe is arranged on the anaerobic/anoxic SBR reactor; the short distance nitration SBR reactor is internally provided with an aerating apparatus; the granular sludge which is processed by acclimatization and has the function of denitrifying dephosphatation is put into the anaerobic/anoxic SBR reactor, and the granular sludge which is processed by acclimatization and has the function of short distance nitration is put into the short distance nitration SBR reactor. The invention solves the problems of unstable effect of nitrogen and phosphorus removal of sewage, high content of effluent suspended matter and lower pass rate, so as to be used for low C/N waste water treatment.

The invention provides a carbon source carrier filler for repairing nitrate pollution of underground water. The carbon source carrier filler is formed by cladding biological ceramsite surface with an organic carbon source coating. The coating of the carbon source carrier filler comprises the following main components of: by weight, 10-50% of a natural carbon source material powder, 10-20% of a solvent, 1-5% of trace element, 10% of animal glue, 0-5% of a deoxidant, 0-5% of a pH conditioning agent and 0-2% of a surfactant. The carbon source carrier filler prepared in the invention has good mechanical properties and biological endophilicity, can achieve the aim of long-acting sustained release of carbon source, is suitable for underground water in-situ and ex-situ remediation technologies, and can be used to effectively avoid the secondary pollution problem of additional carbon sources.

The invention relates to a plant restoration technology for heavy metal-contaminated soil, specifically to a method for repairing heavy metal-contaminated soil by using oilseed rape-oil sunflower crop rotation technology. The method overcomes the problems of seasonal restriction, slow growth, low biomass of an overground part, low total absorption and accumulation amount of heavy metals, tolerance and enrichment capability to only one single heavy metal, bad economic benefits and poor comprehensive effects in practical application of plants used in conventional heavy metal-contaminated soil restoration technology. According to the method provided by the invention, oilseed rape and oil sunflower are planted in soil contaminated by compound heavy metals according to the natural growing periods of the oilseed rape and the oil sunflower, and conventional cultivation is carried out; the oilseed rape and the oil sunflower both grow normally in the contaminated soil, no obvious symptom of toxicity appears, and the outputs of the oilseed rape and the oil sunflower are not affected; and since the biomass of the overground parts of the oilseed rape and the oil sunflower is great, a great amount of various heavy metals can be absorbed and accumulated, and in particular, the contents of Cu, Zn, Cd and Hg in the plants of the oilseed rape and the oil sunflower are obviously higher than soil background values, reaching an enrichment level.

A water eco-environment protection method based on water plant harvesting. The invention relates to a water eco-environment protection method, which is characterized in that water plants are cultivated in a water body or naturally recovered water plants are utilized; nitrogen and phosphorous nutrients are absorbed by water plant growth; the water plants are harvested in growth period by manual work or waterweed harvesting equipment so as to promote the regrowth of the water plants; harvesting is carried out for multiple times, ends in the growth period and is carried out for the last time after the water plants grow seeds or vegetative propagule so as to extract the nitrogen and phosphorus nutrients from water in the form of water plant residues. The invention aims to maximize the accumulated biomass of the water plants during the whole growth period or optimize the water quality purifying effect and simultaneously remove nutrients in the water body and deposits, alleviate the eutrophication process of the water body and prevent the water plants from decaying and polluting the water body once again. The method provided by the invention is suitable for daily ecological maintenance of shallow lakes, urban landscape water, slow flow rivers, small reservoirs, wetlands and ponds.

The invention discloses a method for intercropping a cover plant in glades of cold zones and temperate zones. In the method, smooth brome is mainly planted among tree lines with the crown density of 0.2-0.9, wherein, the distance of the smooth brome from tree stumps is 20-50cm; the specific planting method comprises seeding, weeding, applying fertilizers, cutting and the like. The smooth brome intercropped by the method has the advantages of good cold resistance and drought resistance, strong shade tolerance, perennation and the like, and is applicable to being taken as the cover plant in orchards and artificial woodlands. The smooth brome is taken as the cover plant, which can reduce water loss and soil erosion, improve the population number of natural enemies and reduce the number of pests to reduce damages of the pests to fruit trees or forest trees, and further reduce the consumption of herbicides and pesticides, lower the production cost and reduces the environmental pollution; and the harvested smooth brome is taken as pasture grass, which can also increase the income of farmers.

The present invention discloses a method for repairing soil contaminated by heavy metal by utilizing plant. Said method is characterized by that in the soil contaminated by heavy metal the maize can be planted, before harvest at least two additives selected from editic acid or its sodium salt or its potassium salt (EDTA), nitrilotriacetic acid or its sodium salt or its potassium salt (NTA) and citric aid or its sodium salt or its potassium salt (CA) can be applied, and then the aerial part of maize can be harvested so as to attain the goal of repairing soil contaminated by heavy metal.

The invention belongs to the technical field of soil remediation, and particularly relates to a remediation method of heavy metal contaminated cultivated land, which comprises the following steps: firstly, deeply ploughing soil and uniformly mixing a heavy metal improver with the soil to improve the soil, and then fertilizing the soil. The method provided by the invention can remarkably reduce theeffectiveness of heavy metals in soil and reduce the influence of the heavy metals on the product quality, and can improve the soil fertility and increase the yield and quality of crops by adopting amode of planting green manure or applying an organic fertilizer.