9results about How to "Large biomass" patented technology

This invention discloses *Aphelenchus brittleus* and its applications, relating to the field of microbial technology. The strain is taxonomically named *Aphelenchus brittleus* (…). Acaulospora delicata The specimen, with accession number CGMCC NO.42100, is a *C. cristatum* NM26B that can improve the yield and quality of chives, enhance mineral nutrient absorption, and increase the expression of genes synthesizing chive-specific aroma compounds. This lays the foundation for future research and development of suitable microbial agents for the development of the chive industry.

The invention discloses trichoderma sp. Y3 and application thereof in promoting rice growth, and belongs to the technical field of microorganisms. The classification name of the trichoderma sp. Y3 is Trichoderma sp. Y3, the trichoderma sp. Y3 is preserved in the China Center for Type Culture Collection, and the preservation number is CCTCC NO: M 2026214. And biological characteristics and molecular identification are combined to identify that the trichoderma Y3 belongs to a new trichoderma species. When the strain or a fermentation product thereof is applied to a rice culture medium, the growth of rice can be effectively promoted, and the plant biomass is increased. Trichoderma Y3 is developed and prepared into the biological bacterial fertilizer, and a new way is provided for application of microorganisms to development of crop production.

This invention relates to the fields of transcription factor engineering and metabolic engineering, and discloses an engineered strain that produces L-isoleucine, its construction method, and its applications. This strain... E. coli K12, MG1655 ( E. coli A1) serves as the host, integrating the subunit of the host factor IHF. ihfβ, ihfα Natural promoter replaced with P trc Strong promoters enable IHF overexpression, resulting in engineered bacteria. E. coli IHF4. This invention also discloses the construction steps of this strain, as well as its application method for L-isoleucine production by inoculating it into a fermentation medium, culturing it at 36-38℃ and 200 r / min for 40-60 h, and adjusting the pH and supplementing sugar. This engineered strain increases the yield of L-isoleucine by 42.11% compared to the starting strain. The construction method is simple and suitable for industrial microbial fermentation production of L-isoleucine.

The invention discloses application of modified small nuclear RNA (Ribonucleic Acid) and a protein complex thereof in enhancing plant photosynthesis. The invention relates to the technical field of biology, and provides a small nuclear RNA (Ribonucleic Acid) of which the nucleotide sequence is SEQ ID NO: 1 or SEQ ID NO: 2. The invention also provides a small nuclear RNA-protein complex composed of the small nuclear RNA and an Sm protein specifically bound with the small nuclear RNA. The small nuclear RNA and the protein complex thereof disclosed by the invention can be specifically combined with mRNA 3 'UTR regions of a plurality of nuclear coding chloroplast protein genes through base complementary pairing, and the expression of the nuclear coding chloroplast protein is enhanced on the translation level, so that the biosynthesis of chloroplast is promoted, and the photosynthetic efficiency of plants is improved.

The invention discloses a method for increasing protein content and amino acid content of phaeodactylum tricornutum and application. According to the method, a DPH gene in wild type phaeodactylum tricornutum is knocked out through a gene knockout technology, so that the total protein content and the amino acid content of the phaeodactylum tricornutum are increased, a method for knocking out the DPH gene in the wild type phaeodactylum tricornutum comprises the steps that the DPH gene is knocked out in a targeted mode through a CRISPR / Cas9 editing carrier, the nucleotide sequence of gRNA of the DPH gene is knocked out in a targeted mode through the CRISPR / Cas9 editing carrier is shown as SEQ ID No.3, and the content of the total protein in the wild type phaeodactylum tricornutum is increased. The total protein content and the essential amino acid content of the DPH gene knockout phaeodactylum tricornutum strain are remarkably improved compared with those of a wild type strain. The transformed algal strain can be used as a high-quality protein source to be applied to the field of aquaculture, the nutritional value and the transformation efficiency of feed are effectively improved, and the growth performance and the health condition of bred animals are improved. Wide market prospects and important economic values are realized.

This invention discloses a synergistic method for cold-season grazing and warm-season fertilization in alpine meadows, belonging to the field of alpine grassland ecological restoration and sustainable agricultural management technology. The method includes the following steps: using UAV remote sensing to acquire spatial distribution information of litter in the cold-season grassland, and dividing the grassland into patches with different litter loads based on this information, implementing differentiated grazing by region; using a soil sensor network buried in the grassland to monitor soil temperature and moisture content, dynamically defining the start and end times of cold-season grazing and the operational window for warm-season fertilization; during the warm season, spraying the grassland with liquid nitrogen and phosphorus fertilizer containing synergists according to the warm-season fertilization operational window. This invention deeply couples the physical disturbance of cold-season grazing with the chemical regulation of liquid nutrients in the warm season, solving problems such as slow litter decomposition, low fertilizer utilization, delayed greening, and decreased soil fertility in alpine meadows, achieving a synergistic improvement in grassland production and ecological functions.

This invention discloses a thermosensitive molybdenum disulfide composite nanomaterial and its application in controlling crop chilling injury, belonging to the field of chilling agent technology. The invention utilizes a mixture of monomers (N-isopropylacrylamide and N-tert-butylacrylamide), a crosslinking agent, a surfactant, and a MoS2 dispersion, heated for a period of time; subsequently, an initiator is added, and the reaction continues for a period of time; after the reaction is completed, the mixture is cooled and dialyzed to obtain the thermosensitive molybdenum disulfide composite nanomaterial. This composite nanomaterial is dispersed in water to prepare a suspension, which is then applied to crops such as corn and soybeans via foliar spraying, thereby enhancing their cold resistance and increasing the biomass of chilled crops such as corn and soybeans. The method of this invention is simple and efficient, and has good application prospects.

The application relates to a lighting device and method suitable for greenhouse cultivation, which is arranged in a greenhouse for mixed cultivation of animals and plants. The device comprises a carbon dioxide supply structure for supplementing gas carbon dioxide or organic carbon fertilizer to a plant area; a light source for providing light required by plants for growth; a photosynthesis monitoring unit provided with a first detection unit for detecting a net photosynthetic rate of the plants and a second detection unit for collecting leaf area of the plants; and a control unit in communication connection with the carbon dioxide supply structure, the light source and the photosynthesis monitoring unit. One of the purposes of the application is to provide a system for solving the problem of carbon hunger of plants, which comprises an above-ground carbon amount detection module and an underground carbon amount supply module. The control unit can obtain the carbon amount required to be supplied to the plants by the underground carbon amount supply module based on the total carbon amount required by the plant growth and the carbon conversion amount of the plants under photosynthesis detected by the above-ground carbon amount detection module according to the real-time change of illumination.

This invention provides the application and method of Streptomyces S16-1 in promoting the growth and salt stress tolerance of Leymus chinensis, belonging to the field of microbial technology. Streptomyces S16-1 was isolated from saline soil, with the preservation number CCTCC M 20251940, and its 16S rDNA sequence is shown in SEQ ID NO:1. The Streptomyces S16-1 has functional activities such as salt and alkali tolerance, phosphorus solubilization, nitrogen fixation, and production of 1-aminocyclopropane-1-carboxylic acid (ACC) deaminase. The microbial fertilizer is obtained by expanding the culture of Streptomyces S16-1. Compared with chemical fertilizers, the microbial fertilizer used in this invention is more environmentally friendly. It can not only improve soil fertility and help plants improve their salt and alkali resistance, but also promote the balance and diversity of soil microbial communities, which helps maintain the health and stability of the soil ecosystem.