The invention relates to a method for culturing industrial
cannabinoid and tanshinone high-yield industrial
cannabinoid adventitious roots, and belongs to the technical field of adventitious root cultivation. The method comprises the following steps: performing
induction treatment on the industrial
cannabis sativa adventitious roots by adopting a
yeast extract to obtain the industrial
cannabis sativa adventitious roots subjected to
induction treatment; the
yeast extract is low in cost and abundant in
raw material supply, and can stimulate industrial
cannabis sativa adventitious roots to synthesize more target metabolites as an
elicitor; the industrial cannabis sativa adventitious roots subjected to
induction treatment are subjected to scale-up culture by utilizing a
bioreactor, so that
cell proliferation and growth of the adventitious roots are promoted, and the
biomass of the adventitious roots is increased; meanwhile, the adventitious roots are promoted to accumulate more target metabolites, and at the moment, the yields of
cannabinoid phenol,
cannabidiol, tetrahydrotanshinone I,
tanshinone IIA and
dihydrotanshinone I in the adventitious roots are up to 80.65 mg / L DW, 53.58 mg / L DW, 103.76 mg / L DW, 21.71 mg / L DW and 5.71 mg / L DW respectively. According to the method, the operation steps and the labor intensity are reduced, the defects of low
biomass and low cannabinoid yield in the existing industrial cannabinoid adventitious root culture technology are overcome, and the industrial cannabinoid and tanshinone high-yield industrial cannabinoid adventitious roots can be economically and efficiently cultured all the year round.