19results about How to "Large biomass" patented technology

This invention discloses *Aphelenchus brittleus* and its applications, relating to the field of microbial technology. The strain is taxonomically named *Aphelenchus brittleus* (…). Acaulospora delicata The specimen, with accession number CGMCC NO.42100, is a *C. cristatum* NM26B that can improve the yield and quality of chives, enhance mineral nutrient absorption, and increase the expression of genes synthesizing chive-specific aroma compounds. This lays the foundation for future research and development of suitable microbial agents for the development of the chive industry.

The application provides a method for dynamically regulating alcohol content to enhance the flavor of solid vinegar, comprising the following steps: S1, liquefaction; S2, saccharification; S3, alcohol fermentation; S4, solid-state layered fermentation; and S5, finished product preparation. The vinegar preparation method has the advantages that the fermentation performance is improved, the fermentation period is shortened, the conversion rate of acetic acid is improved, the biomass of bacteria is increased, the growth of acetic acid bacteria is as good as possible, the rate of acetic acid fermentation is improved, the period of acetic acid fermentation is shortened, the flavor of liquid-state fermented vinegar is improved, the irritation of solid-state fermented vinegar is reduced, and the method has a good market application prospect.

The invention discloses trichoderma sp. Y3 and application thereof in promoting rice growth, and belongs to the technical field of microorganisms. The classification name of the trichoderma sp. Y3 is Trichoderma sp. Y3, the trichoderma sp. Y3 is preserved in the China Center for Type Culture Collection, and the preservation number is CCTCC NO: M 2026214. And biological characteristics and molecular identification are combined to identify that the trichoderma Y3 belongs to a new trichoderma species. When the strain or a fermentation product thereof is applied to a rice culture medium, the growth of rice can be effectively promoted, and the plant biomass is increased. Trichoderma Y3 is developed and prepared into the biological bacterial fertilizer, and a new way is provided for application of microorganisms to development of crop production.

This invention relates to the fields of transcription factor engineering and metabolic engineering, and discloses an engineered strain that produces L-isoleucine, its construction method, and its applications. This strain... E. coli K12, MG1655 ( E. coli A1) serves as the host, integrating the subunit of the host factor IHF. ihfβ, ihfα Natural promoter replaced with P trc Strong promoters enable IHF overexpression, resulting in engineered bacteria. E. coli IHF4. This invention also discloses the construction steps of this strain, as well as its application method for L-isoleucine production by inoculating it into a fermentation medium, culturing it at 36-38℃ and 200 r / min for 40-60 h, and adjusting the pH and supplementing sugar. This engineered strain increases the yield of L-isoleucine by 42.11% compared to the starting strain. The construction method is simple and suitable for industrial microbial fermentation production of L-isoleucine.

The invention discloses application of modified small nuclear RNA (Ribonucleic Acid) and a protein complex thereof in enhancing plant photosynthesis. The invention relates to the technical field of biology, and provides a small nuclear RNA (Ribonucleic Acid) of which the nucleotide sequence is SEQ ID NO: 1 or SEQ ID NO: 2. The invention also provides a small nuclear RNA-protein complex composed of the small nuclear RNA and an Sm protein specifically bound with the small nuclear RNA. The small nuclear RNA and the protein complex thereof disclosed by the invention can be specifically combined with mRNA 3 'UTR regions of a plurality of nuclear coding chloroplast protein genes through base complementary pairing, and the expression of the nuclear coding chloroplast protein is enhanced on the translation level, so that the biosynthesis of chloroplast is promoted, and the photosynthetic efficiency of plants is improved.

The invention belongs to the field of plant genetic engineering, and provides a nucleotide molecule, a recombinant vector and a recombinant host cell for silencing a squalene epoxidase gene NtSQE1 in tobacco and application of the nucleotide molecule, the recombinant vector and the recombinant host cell in increasing the squalene content in the tobacco, and the SEQ ID NO: 2 sequence in the squalene epoxidase gene NtSQE1 is inhibited in a targeted mode through a specific virus-induced gene silencing technology (VIGS). The accumulation level of squalene in a plant body can be remarkably improved under the condition that the plant phenotype is not influenced, and the silencing efficiency is high.

The invention provides a breeding application of a cotton GhD05G2577 gene in increasing the plant germination rate, the biomass, the seed size and the thousand seed weight. Through a transgenic technology, an arabidopsis thaliana homozygous line plant over-expressing the GhD05G2577 gene is obtained. Compared with a wild strain, the early germination rate of a transgenic plant in a 1 / 2 MS plate is obviously increased; in the seedling stage, the leaf number is increased, the leaf length and the leaf width are increased, the plant height is increased, the chlorophyll content SPAD is increased, and the fresh weight, the dry matter content and the biomass accumulation are remarkably increased; the fruit shows that the legume length and width are increased, the seed length and width are increased, and the thousand seed weight is increased, and all reach a significant level. When the gene is applied to high-yield and income-increasing breeding of plants, the germination rate of the plants can be increased, biomass accumulation of the plants can be increased, the seed size and thousand seed weight can be increased, and an alternative scheme is effectively provided for high-yield molecular breeding of the plants.

The invention discloses a method for increasing protein content and amino acid content of phaeodactylum tricornutum and application. According to the method, a DPH gene in wild type phaeodactylum tricornutum is knocked out through a gene knockout technology, so that the total protein content and the amino acid content of the phaeodactylum tricornutum are increased, a method for knocking out the DPH gene in the wild type phaeodactylum tricornutum comprises the steps that the DPH gene is knocked out in a targeted mode through a CRISPR / Cas9 editing carrier, the nucleotide sequence of gRNA of the DPH gene is knocked out in a targeted mode through the CRISPR / Cas9 editing carrier is shown as SEQ ID No.3, and the content of the total protein in the wild type phaeodactylum tricornutum is increased. The total protein content and the essential amino acid content of the DPH gene knockout phaeodactylum tricornutum strain are remarkably improved compared with those of a wild type strain. The transformed algal strain can be used as a high-quality protein source to be applied to the field of aquaculture, the nutritional value and the transformation efficiency of feed are effectively improved, and the growth performance and the health condition of bred animals are improved. Wide market prospects and important economic values are realized.

The invention discloses trachycarpus racemosus and application thereof and a preparation method of fungal chitosan, and belongs to the technical field of biology and application thereof. The strain is preserved in the China General Microbiological Culture Collection Center (CGMCC), the preservation number is CGMCC No.42357, and the preservation date is December 10, 2025. The bacterial strain disclosed by the invention is Lichtheimia ramosa BT-0-A-001-JZ-001 separated from a wine brewing environment, and fungal chitosan with high purity and high deacetylation degree can be extracted by culturing the Lichtheimia ramosa BT-0-A-001-JZ-001. As an effective source of chitosan, the problem that traditional chitosan extraction excessively depends on fishery resources is solved, and the problem that raw materials for producing chitosan are limited is solved.

The invention belongs to the technical field of microorganisms, and particularly relates to a compost pseudomonas, a microbial agent and application of the compost pseudomonas, the compost pseudomonas is P430, the compost pseudomonas is preserved in the China Center for Type Culture Collection, the preservation date is November 18, 2024, the preservation number is CCTCC NO: M 20242583, the strain has high siderophore production activity, the siderophore activity unit is 44.2% in 72 h, and the siderophore production time is 24 h. Under salt stress, the soybean emergence rate can be remarkably increased, soybean seedling growth can be remarkably promoted, and the inhibition effect of salt stress on rape radicle and bud growth can be remarkably relieved.

The invention belongs to the technical field of water body pollution treatment, and discloses black and odorous water body in-situ remediation and water quality maintenance equipment which comprises a plurality of gas transmission main pipes laid in a water body. The aeration equipment is communicated with the gas transmission main pipe and is used for generating bubbles in a water body; the aquatic plant support is located in the water body and fixedly connected to the air conveying main pipe, and the aquatic plant support is located above the aeration equipment; the bottom of the artificial aquatic plant is fixedly connected to the top end of the aquatic plant support, the upper portion of the artificial aquatic plant suspends in a water body, and the artificial aquatic plant is used for attaching microorganisms. The method is few in use limiting factors, does not occupy rivers, does not affect flood discharge, is high in oxygen utilization rate, is better in microorganism enrichment growth environment, and can realize efficient treatment of black and odorous water and long-acting maintenance of water quality.

The application discloses a water quality purifying device for water body treatment, and relates to the technical field of water quality purification, which comprises a water quality purifier arranged in a river channel, wherein the water quality purifier comprises two groups of first frame bodies and second frame bodies arranged in parallel and symmetrically; the first frame bodies and the second frame bodies are fixedly connected through two groups of connecting frames arranged in parallel and symmetrically; the first frame bodies, the second frame bodies and the connecting frames enclose a purifier frame; wherein a plurality of groups of biological filler supports are arranged in an array mode in the purifier frame; an adjusting module is further arranged in the purifier frame, and the adjusting module is used for adjusting the spacing of the biological filler supports; the spacing of the biological filler supports of each layer can be adjusted in real time through the adjusting module, so that the spacing of the biological filler supports of each layer is larger; therefore, the water flow can flow in the gaps between the biological filler supports, the blocking of the water flow by the biological filler supports is reduced, the stability of the water quality purifier in the river channel is improved, and the water quality purifier is prevented from being washed away or washed out.

This invention discloses a synergistic method for cold-season grazing and warm-season fertilization in alpine meadows, belonging to the field of alpine grassland ecological restoration and sustainable agricultural management technology. The method includes the following steps: using UAV remote sensing to acquire spatial distribution information of litter in the cold-season grassland, and dividing the grassland into patches with different litter loads based on this information, implementing differentiated grazing by region; using a soil sensor network buried in the grassland to monitor soil temperature and moisture content, dynamically defining the start and end times of cold-season grazing and the operational window for warm-season fertilization; during the warm season, spraying the grassland with liquid nitrogen and phosphorus fertilizer containing synergists according to the warm-season fertilization operational window. This invention deeply couples the physical disturbance of cold-season grazing with the chemical regulation of liquid nutrients in the warm season, solving problems such as slow litter decomposition, low fertilizer utilization, delayed greening, and decreased soil fertility in alpine meadows, achieving a synergistic improvement in grassland production and ecological functions.

This invention discloses a thermosensitive molybdenum disulfide composite nanomaterial and its application in controlling crop chilling injury, belonging to the field of chilling agent technology. The invention utilizes a mixture of monomers (N-isopropylacrylamide and N-tert-butylacrylamide), a crosslinking agent, a surfactant, and a MoS2 dispersion, heated for a period of time; subsequently, an initiator is added, and the reaction continues for a period of time; after the reaction is completed, the mixture is cooled and dialyzed to obtain the thermosensitive molybdenum disulfide composite nanomaterial. This composite nanomaterial is dispersed in water to prepare a suspension, which is then applied to crops such as corn and soybeans via foliar spraying, thereby enhancing their cold resistance and increasing the biomass of chilled crops such as corn and soybeans. The method of this invention is simple and efficient, and has good application prospects.

The invention discloses a method for improving the nitrogen utilization efficiency of cucumbers and promoting growth by utilizing piriformospora indica and application, and relates to the technical field of agricultural microbial technologies and plant cultivation. According to the invention, the piriformospora indica is inoculated into a cucumber cultivation system to establish a symbiotic relationship with a cucumber root system, so that the biomass, chlorophyll content and nitrogen content of the cucumber under normal nitrogen and low nitrogen conditions can be remarkably increased. The molecular mechanism of the piriformospora indica is as follows: the piriformospora indica can obviously up-regulate the expression of a plurality of nitrogen transport protein genes (CsNPF / CsNRT2) in the root system of the cucumber, and particularly negatively regulate the expression of transcription factors CsbZIP55 and CsbZIP65 under a low-nitrogen condition, so that the inhibition effect of the piriformospora indica on downstream CsNPF genes is relieved, and nitrogen absorption is promoted. The invention provides a new technical scheme and theoretical basis for developing an environment-friendly cucumber growth-promoting microbial preparation and realizing low-nitrogen efficient cultivation.

The application relates to a lighting device and method suitable for greenhouse cultivation, which is arranged in a greenhouse for mixed cultivation of animals and plants. The device comprises a carbon dioxide supply structure for supplementing gas carbon dioxide or organic carbon fertilizer to a plant area; a light source for providing light required by plants for growth; a photosynthesis monitoring unit provided with a first detection unit for detecting a net photosynthetic rate of the plants and a second detection unit for collecting leaf area of the plants; and a control unit in communication connection with the carbon dioxide supply structure, the light source and the photosynthesis monitoring unit. One of the purposes of the application is to provide a system for solving the problem of carbon hunger of plants, which comprises an above-ground carbon amount detection module and an underground carbon amount supply module. The control unit can obtain the carbon amount required to be supplied to the plants by the underground carbon amount supply module based on the total carbon amount required by the plant growth and the carbon conversion amount of the plants under photosynthesis detected by the above-ground carbon amount detection module according to the real-time change of illumination.

This invention provides the application and method of Streptomyces S16-1 in promoting the growth and salt stress tolerance of Leymus chinensis, belonging to the field of microbial technology. Streptomyces S16-1 was isolated from saline soil, with the preservation number CCTCC M 20251940, and its 16S rDNA sequence is shown in SEQ ID NO:1. The Streptomyces S16-1 has functional activities such as salt and alkali tolerance, phosphorus solubilization, nitrogen fixation, and production of 1-aminocyclopropane-1-carboxylic acid (ACC) deaminase. The microbial fertilizer is obtained by expanding the culture of Streptomyces S16-1. Compared with chemical fertilizers, the microbial fertilizer used in this invention is more environmentally friendly. It can not only improve soil fertility and help plants improve their salt and alkali resistance, but also promote the balance and diversity of soil microbial communities, which helps maintain the health and stability of the soil ecosystem.

The invention belongs to the field of plant genetic engineering, and provides a nucleotide molecule, a recombinant vector and a recombinant host cell for silencing a squalene epoxidase gene NtSQU2 of tobacco and application of the nucleotide molecule, the recombinant vector and the recombinant host cell in increasing the squalene content in the tobacco, and the sequence SEQ ID NO: 3 in the squalene epoxidase gene NtSQU2 is inhibited in a targeted mode through a specific virus-induced gene silencing technology (VIGS). The accumulation level of squalene in a plant body can be remarkably improved under the condition that the plant phenotype is not influenced, and the silencing efficiency is high.

The invention belongs to the field of contaminated soil treatment, and particularly relates to a method for phytoremediation of petroleum-contaminated soil, which comprises the step of planting cornflower and / or aster on the petroleum-contaminated soil for soil remediation. According to the method, the cornflower and / or the radix asteris are / is adopted for remediation of the petroleum-contaminated soil, the degradation rate of petroleum in the petroleum-contaminated soil is high, the degradation rate of petroleum in the soil of 12%, 8% and 4% of the petroleum-contaminated soil and the degradation rate of petroleum in the soil of the radix asteris are 56.94%, 84.72% and 86.33% respectively, and the degradation rate of cornflower to petroleum in the soil is 73.89%, 79.60% and 87.30% respectively and is higher than that of cornflower in most of existing remediation methods. Flowers of the cornflower and the tatarian aster are beautiful and large in biomass and can be planted in a petroleum pollution area to form a flower sea, and the target of petroleum pollution remediation is achieved while viewing is achieved.