Salt-tolerant growth-promoting bacteria strain, screening method and application thereof

A technology of promoting bacteria and strains, applied in the field of microbial separation and application, can solve the problems of poor stability and immature microbial separation and purification technology, and achieve the effects of improving salt tolerance, increasing salt tolerance and promoting growth, and promoting growth.

Inactive Publication Date: 2019-01-18
SUZHOU POLYTECHNIC INST OF AGRI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Most rhizosphere microorganisms are harmless to plants and can promote plant growth. These rhizosphere microorganisms have the functions of fixing nitrogen, dissolving phosphorus, producing plant growth hormones, siderophores, and inducing plant resistance. Using rhizosphere microorganisms to improve crop tolerance in saline-alkali land Salt growth-promoting methods have the characteristics of less investment, quick results, and high benefits. The research on the functional activity of rhizosphere microorganisms for salt-tolerance and growth-promoting has attracted extensive attention from relevant researchers. poor sex

Method used

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  • Salt-tolerant growth-promoting bacteria strain, screening method and application thereof
  • Salt-tolerant growth-promoting bacteria strain, screening method and application thereof
  • Salt-tolerant growth-promoting bacteria strain, screening method and application thereof

Examples

Experimental program
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Embodiment 1

[0054] Step 1. Isolation and screening of salt-tolerant growth-promoting bacterial strains

[0055] On July 6, 2017, 15 soil samples were collected from the saline-alkali soil along the coast of Yancheng, Jiangsu. 1g of each soil sample was suspended in 100ml sterile water and diluted to 105 times. The NaCl concentration was 2%, 4%, 6% and 8% NA medium to screen salt-tolerant growth-promoting bacterial strains step by step, specifically: use a pipette to take 0.1ml of the soil dilution solution and spread it on the medium plate, and culture it at 29-31°C for 2-3 days , select a colony that grows well at a salt concentration of 8%, and save it for future use after repeated streaking and purification. A single colony is screened, a salt-tolerant growth-promoting bacterial strain B7, such as figure 1 shown.

[0056] Step 2. Identification of salt-tolerant growth-promoting bacterial strains

[0057] Bacterial molecular genetics classification and identification of the screened s...

Embodiment 2

[0074] A bacterial strain prepared by a salt-tolerant growth-promoting bacteria strain, comprising the following steps:

[0075] Pick a single colony of the salt-tolerant growth-promoting bacteria strain B7 that has grown on the LB solid plate for 48 hours and inoculate it in 250ml LB liquid medium, in a shaking flask at a temperature of 29-31°C and a rotation speed of 180-210r / min After culturing for 48 hours, wait for the bacteria to grow to the logarithmic growth phase, put them into a sterile centrifuge tube, centrifuge at 12000 rpm for 10 minutes, discard the supernatant, collect the strains in the lower layer, wash the bacteria twice with sterile water, and use sterile The OD value of the water-adjusted bacteria is 0.5-0.8, which is used as a strain liquid for plant salt tolerance and growth promotion.

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Abstract

The invention provides a salt-tolerant growth-promoting bacteria strain, a screening method and the application thereof. Salt-tolerant and growth-promoting bacteria strains were screened from saline-alkaline soil, the genetic characteristics and physiological and biochemical characteristics of the strain were determined, The strain was identified as Bacillus megaterium B7, which was deposited in China Typical Culture Collection Center on May 17, 2018. Salt tolerance analysis was carried out to test the salt tolerance and growth promotion of cucumber seeds under salt stress and pot experiments.The salt-tolerant growth-promoting bacterial strain provided by the invention, Screening Method and Application, the salt tolerance of Bacillus megaterium B7 is 0-8%, while producing IAA, ACC deaminase, ammonia gas, nitrogen fixation and phosphorus removal were produced. Bacillus megaterium could significantly improve salt tolerance and growth of cucumber under salt stress, slow down salt injury,increase biomass and reduce the use of chemical fertilizers, and could be used to prepare bacterial solution to promote crop germination and growth under salt stress.

Description

technical field [0001] The invention belongs to the technical field of microorganism isolation and application, and in particular relates to a salt-tolerant growth-promoting bacterial strain, a screening method and application. Background technique [0002] At present, with the development of society and the increase of population, the demand for cultivated land resources is also increasing. Soil salinization refers to the process in which the salt in the bottom soil or groundwater rises to the surface with capillary water, and after the water evaporates, the salt accumulates in the surface soil. The phenomenon or process of soluble salt accumulation on the soil surface, also known as salinization. In addition to coastal areas, where salinization occurs due to seawater immersion, facility agriculture in arid and semi-arid areas is also an important cause of secondary soil salinization. Soil salinization is one of the major abiotic stress factors currently threatening globa...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12Q1/689A01N63/00A01P21/00C12R1/11
CPCA01N63/00C12N1/02C12N1/20C12Q1/689C12N1/205C12R2001/11
Inventor 钱兰华李婧冀小草王桃云李良智扶教龙钱玮严立石
Owner SUZHOU POLYTECHNIC INST OF AGRI
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