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A Salt-resistant Potassium Promoting Microbacterium Oxygenans 41c8, Bacterial Agent and Its Application

A technology of oxidative microbacteria and growth-promoting bacteria, applied in the field of microorganisms, can solve the problems of lack of salt-tolerant growth-promoting microorganisms, lack of high-salt-tolerant growth-promoting microorganisms, etc. The effect of the application foreground

Active Publication Date: 2022-03-25
QINGDAO AGRI UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the prior art, there are few studies on salt-tolerant growth-promoting microorganisms, and there is a lack of salt-tolerant growth-promoting microorganisms, especially the lack of effective high-salt-tolerant growth-promoting microorganisms

Method used

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  • A Salt-resistant Potassium Promoting Microbacterium Oxygenans 41c8, Bacterial Agent and Its Application
  • A Salt-resistant Potassium Promoting Microbacterium Oxygenans 41c8, Bacterial Agent and Its Application
  • A Salt-resistant Potassium Promoting Microbacterium Oxygenans 41c8, Bacterial Agent and Its Application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] Example 1 Separation screening of strain 41c8

[0049] Different microorganisms were separated from the soil in Dongying City, and the strains were separated from the solid Lb medium plate from which the strain was isolated from the solid Lb culture plate. The colonies were placed in a liquid LB medium with a concentration of 0.1%, 0.3%, 0.5% and 0.7% different NaCl concentrations for shake flask culture, from 27 ° C to 29 ° C 120R / min culture 2 ~ 3D, the production of strains under different salt concentrations was observed, and the rated plastered strains of the screening were repeatedly paired, and the salt-tolerant strain of five single colonies was obtained, and the single colonies were named strain 41c8.

Embodiment 2

[0050] Example 2 functional qualitative identification of strains 41c8

[0051] The strain 41c8 is cultured, activated and expanded, and formulated with pitcassing, phosphorus and nitrogen solid medium, and prepared a flat plate of the bacteria. After culturing the bacteria, the plate is replaced, Thereafter, 1 μl of the bacteria is supreme onto the plate, and the sealing plate is placed in inverted culture, and the experimental results are observed when the transparent water is unpowered or plaque. Such as figure 2 As shown, the strain 41c8 of the present embodiment can be soluble organophos, difficult soluble potassium and nitrogen. Organic phosphorus medium, inorganic phosphorus medium, pita-potassium medium and nitrogen nitrogen medium are as follows.

[0052] Organophosphorus medium: glucose 10g, NaCl 0.3g, MgSO 4 0.3 g, MNSO 4 0.03 g, Caco 3 5 g, lecithin 2g, agar 18g, (NH 4 ) 2 SO 4 0.5 g, KCl 0.3g, FESO 4 0.03 g, yeast paste 0.4 g, 1000 ml of distilled water, pH 7.0-7.5, 1...

Embodiment 3

[0058] Example 3 Fungi, bacteria and supernatant of bacterial liquid, bacteria and supernatant

[0059] Potassium analysis of 1 strain 41C8

[0060] Different processing Colony diameter (d) / cm Water solution diameter (d) / cm Cosmetic index (D / D) Bacterial liquid 0.9 1.5 1.67 Bacterial 0.8 1 1.25 Supernatant 0.7 0.9 1.29 Sterilizing fluid - - -

[0061] Prepare the potassium solid medium plate, further xylosomic analysis of strain 41C8, the strain 41C8 was cultured, and the bacterial liquid was taken to the centrifuge tube, 5000 r / min for 2 minutes, and the supernatant The liquid is taken into the new centrifuge tube, and the remaining bacteria is added to the sterile water. Then 1 μl of bacterial liquid, supernatant, sterilized bacterial liquid and bacterial suspension to prepared xa-potassium solid medium, placed in a 25 ° C incubator culture, to a plate long out of water, and a plaque statistical experiment .

[0062] Calculatio...

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Abstract

The present invention provides a strain of salt-resistant and potassium-promoting oxidative microbacterium 41C8, which was preserved in the General Microbiology Center of China Microbiological Culture Collection Management Committee on January 4, 2021, with a preservation registration number of CGMCC No.21585. The invention can be applied to dissolving potassium, fixing nitrogen, dissolving phosphorus and promoting plant growth. The invention also provides a microbial bacterial agent including Microbacterium oxidans 41C8 and a preparation method thereof. The strain has a salinity tolerance of 0 to 7%, and the optimum salinity tolerance is 3 to 5%. It also has various growth-promoting characteristics such as nitrogen fixation, organic phosphorus and potassium dissolution, and the bacterial solution prepared by Microbacterium oxidans 41C8 can be used in salt water. Significantly improve the salt-tolerant growth-promoting ability of cabbage under stress, slow down the phenomenon of salt damage, increase the biomass, thereby promoting the growth of cabbage, help to improve the salt-tolerance of cabbage, reduce the use of chemical fertilizers, the salt-tolerant growth-promoting bacterial strain It has broad application prospects.

Description

Technical field [0001] The present invention belongs to the field of microorganisms, and specifically, a strain of salvation, potassium, potassium-proactive oxygen, 51c8, fungicide, and its application thereof. Background technique [0002] my country's salt alkali area is 99.13 million hm 2 About 10% of the total area of ​​salt bases in the world. Soil salinization gives great disorders to agricultural production, and salt stress is easily caused by water loss, ion imbalance, nutrient loss, etc., is still one of the main factors that affect crops production and quality worldwide, easy to cause crops. Metabolic disorders such as the imbalance of active oxygen generation and removal, photosynthetic decline, etc., resulting in a significant reduction in the output of facility crops and decrease in quality. The salt streats affect the nutrient absorption of the plant, producing osmotic stress, reducing the photosynthesis of plants, and also produces oxidative stress to inhibit plant...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A01N63/20A01P21/00C12R1/01
CPCC12N1/20A01N63/20
Inventor 刘新刘广超郭艳苹叶青白洋张婧赢李雅华
Owner QINGDAO AGRI UNIV
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