Sinomicrobium strain SM8 and application thereof in salt-tolerant growth promotion

A technology of Microbacteria sinensis and Microbacteria, which is applied in microorganism-based methods, applications, microorganisms, etc., can solve the problems of poor adaptability and poor effect of related microorganisms, and achieve the effects of promoting crop growth, simple production and improved germination rate.

Active Publication Date: 2019-04-05
INST OF SOIL & FERTILIZER XINJIANG ACAD OF AGRI SCI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Although a large number of plant growth-promoting bacteria have been discovered at home and abroad, due to the arid environment and diverse salinity in Xinjiang, there are problems of poor adaptability and poor effect of related microorganisms.

Method used

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  • Sinomicrobium strain SM8 and application thereof in salt-tolerant growth promotion
  • Sinomicrobium strain SM8 and application thereof in salt-tolerant growth promotion
  • Sinomicrobium strain SM8 and application thereof in salt-tolerant growth promotion

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Example 1 Isolation, screening, purification and preservation of Microbacterium sinensis SM8 CGMCC No.15757 strain

[0031] The rhizosphere soil samples of halophytes in Xinjiang Heshuo area were collected, and microorganisms were isolated and purified by gradient dilution method. Weigh 10g of the soil sample, put it into 100ml of sterile water (with glass beads added), shake at 200rpm, 30°C for 20min, to fully disperse the sample. After serial dilution, dilute with sterile water to prepare 10 -2 -10 -5 sample suspension diluent. Take 100 μL of the dilutions of each concentration, apply the conventional coating method on the TSA solid medium plate containing 2% NaCl, and place it in a constant temperature incubator at 30°C for cultivation. After 48 hours, after colonies grow on the plate, pick a single colony on the plate for purification and culture. For the purified single colonies ( figure 1 ), transferred to the slant of TSA medium containing 2% NaCl and stored...

Embodiment 2

[0032] Example 2 Identification of Microbacterium sinensis SM8 CGMCC No.15757 strain

[0033] Sequence determination and analysis of 16S rDNA of a new strain of Microbacterium sinensis CGMCC No.15757:

[0034] (1) Extraction of PCR template DNA:

[0035] The purified strain IS7 was inoculated into 2% NaCl TSA medium, cultured on a shaker at 30°C for 2 days, the bacteria were collected, and the total genomic DNA was extracted using a DNA extraction kit.

[0036] (2) PCR amplification

[0037] Using specific primers:

[0038] 27F: 5'-AGAGTTTGATCCTGGCTCAG-3',

[0039] 1492R:5'-GGTTACCTTGTTACGACTT-3';

[0040] The total volume of the PCR reaction system was 25 μL, and the PCR amplification conditions were as follows: 94°C for 5 minutes; 94°C for 45s, 56°C for 45s, 72°C for 45s, 30 cycles; 72°C for 10 minutes.

[0041] (3) Sequence determination

[0042] The PCR amplified product was detected by electrophoresis and purified and then sequenced to obtain a 16S rDNA sequence wit...

Embodiment 3

[0045] Example 3 Detection of Salt Tolerance and Growth-promoting Ability of Microbacterium sinensis SM8 CGMCC No.15757 Strain

[0046] After adding 10% NaCl to the TSA medium, invert the plate and inoculate with Microbacterium sinensis SM8 CGMCC No.15757. Cultivate at 30°C, and observe the growth of the bacteria for 2-5 days.

[0047] Microbacterium sinensis SM8 CGMCC No.15757 was inoculated on a phosphate-solubilizing (inorganic and organic phosphate) bacterial culture medium at 30°C. After culturing for one week, observe the size of the transparent circle.

[0048] Microbacterium sinensis SM8 CGMCC No.15757 was inoculated in TSA liquid medium containing L-tryptophan (100mg / L) in 2% NaCl, cultured on a shaker (30°C, 180r / min) for 1d, and 50 μL of bacteria The suspension was dropped on a white ceramic plate, and an equal volume of Salkowski colorimetric solution (50mL 35% HClO 4 +1mL 0.5mol / L FeCl 3 ), and a mixed solution of 50 μL of uninoculated 2% TSA liquid medium and ...

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Abstract

The invention provides a sinomicrobium new strain SM8 CGMCC (China General Microbiological Culture Collection Center) No. 15757. The sinomicrobium new strain SM8 CGMCC No. 15757 provided by the invention has the characteristic of growing at both low temperature and high temperature, has high salinity resistance and high pH (Potential of Hydrogen) resistance, is capable of converting insoluble phosphorus in soil into available phosphorus can be directly absorbed and utilized by plants, and has the functions of synthesizing indoleacetic acid, producing ACC (1-Aminocyclopropane-1-Carboxylic Acid)ammonialyase and promoting the growth of the plants. Through the use of related fungicides, the germination rate and the growth speed of cotton under salt stress can be obviously increased, and an obvious salt-tolerant growth promotion function is obtained; not only the using amount of chemical fertilizer can be reduced, but also the expenditure of farmers can be reduced, the economic income canbe increased, and good ecologic benefit and good social benefit are also obtained.

Description

Technical field: [0001] The invention relates to a strain of Microbacterium sinensis SM8 with salt-tolerant growth-promoting effect and its application in improving the salt stress tolerance of crops and promoting the growth of crops at seedling stage, and belongs to the technical field of microbial strains and their applications. Background technique: [0002] Saline-alkali soil is a general term for soils with different degrees of salinization and alkalization. Among them, the soil with a soluble salt content of more than 0.6-2% in the topsoil is called saline soil; there are more exchangeable sodium ions in the soil colloid (the degree of alkalization reaches 15% or 20%), which is alkaline (pH8.5-11.0 ) soils are called alkaline soils. According to the incomplete statistics of UNESCO and FAO, the area of ​​saline-alkali land in the world is 950 million hectares. Among them, my country accounts for more than 10% of the world, and the area of ​​saline-alkali land reaches ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01G7/06C12R1/01
CPCA01G7/06C12R2001/01C12N1/205Y02A40/22
Inventor 徐万里楚敏张志东顾美英朱静唐琦勇唐光木宋素琴
Owner INST OF SOIL & FERTILIZER XINJIANG ACAD OF AGRI SCI
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