1053 results about "Genomic DNA" patented technology

The present disclosure relates to materials and methods for spatially analyzing nucleic acids that have been fragmented with a transposase enzyme, alone or in combination with other types of analytes.

The present disclosure relates to materials and methods for spatially analyzing nucleic acids that have been fragmented with a transposase enzyme, alone or in combination with other types of analytes.

The present disclosure relates to materials and methods for spatially analyzing nucleic acids that have been fragmented with a transposase enzyme, alone or in combination with other types of analytes.

The present disclosure relates to materials and methods for spatially analyzing nucleic acids that have been fragmented with a transposase enzyme, alone or in combination with other types of analytes.

The present disclosure relates to materials and methods for spatially analyzing nucleic acids that have been fragmented with a transposase enzyme, alone or in combination with other types of analytes.

The present disclosure relates to materials and methods for spatially analyzing nucleic acids that have been fragmented with a transposase enzyme, alone or in combination with other types of analytes.

The present disclosure relates to materials and methods for spatially analyzing nucleic acids that have been fragmented with a transposase enzyme, alone or in combination with other types of analytes.

The present disclosure relates to materials and methods for spatially analyzing nucleic acids that have been fragmented with a transposase enzyme, alone or in combination with other types of analytes.

The invention discloses an SNP (Single Nucleotide Polymorphism) molecular marker related to growth traits of litopenaeus vannamei and application of the SNP molecular marker, and belongs to the technical field of biology. The SNP molecular markers 1-3 are remarkably related to the growth traits of the litopenaeus vannamei, genomic DNA of muscular tissue of the litopenaeus vannamei to be detected is extracted and then serves as template DNA to be subjected to PCR amplification and sequencing, the genotypes of the three molecular markers are determined, and the litopenaeus vannamei with obvious growth advantages can be rapidly screened out. The molecular marker can accelerate the breeding process of the improved variety of the litopenaeus vannamei, and provides a theoretical basis for mining application of related genetic markers in litopenaeus vannamei molecular marker-assisted breeding.

The embodiment of the invention discloses a KASP molecular marker, a primer pair and a kit for identifying the dwarf character of muskmelon and application of the KASP molecular marker, relates to the technical field of molecular markers, and aims to promote gene positioning and molecular-assisted breeding processes by utilizing molecular marker-assisted selective breeding on the dwarf character of muskmelon. The method for identifying the dwarf character of the muskmelon comprises the following steps: extracting genome DNA of a target muskmelon, taking the genome DNA of the target muskmelon as a template for PCR amplification, and performing fluorescence detection based on a primer of a KASP marker K1069930 to obtain a gene type of the target muskmelon, on the basis of the detected gene type, the character of the target muskmelon is judged; the KASP marker K1069930 is designed according to an SNP (Single Nucleotide Polymorphism) site at Chr07: 1069930, and the nucleotide sequence of the SNP site is shown as SEQ ID NO. 1. The invention also discloses a kit for detecting the KASP marker K1069930 and a kit for detecting the KASP marker K1069930. The identification method provided by the invention can be more efficiently applied to breeding work of dwarf muskmelon germplasm.

The present disclosure provides compositions, methods, systems, and devices for polynucleotide processing and analyte characterization. Such polynucleotide processing may be useful for a variety of applications, including analyte characterization by polynucleotide sequencing. The compositions, methods, systems, and devices disclosed herein generally describe barcoded oligonucleotides, which can be bound to a bead, such as a gel bead, useful for characterizing one or more analytes including, for example, protein (e.g., cell surface or intracellular proteins), genomic DNA, and RNA (e.g., mRNA or CRISPR guide RNAs). Also described herein, are barcoded labelling agents and oligonucleotide molecules useful for “tagging” analytes for characterization.

The invention discloses a visual detection method of New Delhi tomato leaf curl virus, and belongs to a plant virus detection technology in the field of biotechnology. The method comprises the following steps: adding genome DNA extracted from a plant sample to be detected into an RAA reaction system containing an RAA primer, and carrying out isothermal amplification; then adding an isothermal amplification product into a CRISPR / Cas12a detection system containing a specific crRNA fluorescent probe and an ssDNA fluorescent probe, and carrying out a reaction; and finally, carrying out fluorescence signal detection on the reaction product, if the fluorescence signal is positive, indicating that the New Delhi tomato leaf curl virus is detected in the plant sample, otherwise, indicating that the New Delhi tomato leaf curl virus is not detected. The method has the advantages of being good in specificity, short in detection time, high in sensitivity, free of dependence on expensive instruments and the like, and the result can be accurately detected through an instrument or directly and preliminarily interpreted in a field with limited conditions.

The invention discloses a specific molecular marker, primers, a method and application for genetic sex identification of siniperca chuatsi, the specific molecular marker has two sequences in male fish and has one sequence in female fish, the primers comprise an upstream primer F and a downstream primer R. The primers are adopted to carry out PCR amplification on genome DNA of the siniperca chuatsi to be detected, and the specific molecular marker is obtained. And identifying the genetic sex of the siniperca chuatsi according to an agarose gel electrophoresis detection result. The invention further discloses application of a reagent, a primer, a kit or a method for detecting the specific molecular marker in genetic sex identification of siniperca chuatsi, artificial breeding of the siniperca chuatsi or all-female breeding of the siniperca chuatsi. The siniperca chuatsi genetic sex identification method has the advantages that sex-related male and female differential fragments are found through combined comparative analysis of male and female genomes, primers are designed for amplification verification, only a small amount of DNA of a to-be-detected sample genome is needed as a template, the genetic sex of the siniperca chuatsi can be accurately, simply, conveniently, quickly and stably identified only through PCR and agarose gel electrophoresis, and the method is not limited by the development stage of the siniperca chuatsi.

The invention relates to an IGF2BP3 gene molecular marker primer related to pigeon weight traits and application of the IGF2BP3 gene molecular marker primer, and belongs to the technical field of biology. According to the invention, genome DNA of a to-be-detected pigeon is taken as a template, a specific primer is adopted to carry out PCR amplification on the genome DNA, then a PCR amplification product is subjected to Sanger sequencing and SNP molecular marker genotyping, and then pigeon body weight character selection is carried out. The weight of G / G genotype pigeons is higher than that of A / G and A / A genotype individuals, and the weight of A / G genotype pigeons is higher than that of A / A genotype individuals. By eliminating A / G and A / A genotype individuals and retaining G / G genotype individuals, the molecular marker has the beneficial effects that the molecular marker is used as a genetic marker for breeding pigeons, and the pigeons with large weight and uniformity are bred; the method can efficiently and quickly identify the weight traits of the pigeons, improves the uniformity of the weight of pigeons, provides a scientific basis for the early breeding of the pigeons, and better serves the breeding of the pigeons.

To provide a technique that enables direct seeding cultivation of seed potatoes in the field of production of sweet potato tuberous roots (sweet potato cultivation), which offers significant labor-saving benefits, wherein the technique enables efficient development of sweet potato plants having desired traits and exhibiting low enlargement of seed potatoes.SOLUTION: A method for identifying a sweet potato plant exhibiting low enlargement of seed potatoes, includes the steps of: detecting a nucleotide site indicating the "Kyushu 199-type variant allele" associated with a DNA marker from a genomic DNA of the sweet potato plant to be identified, with respect to "the DNA marker that shows relevance to the phenotype of low enlargement of seed potatoes" in the genomic DNA of sweet potato plants; and determining that the sweet potato plant to be identified is a sweet potato plant exhibiting low enlargement of seed potatoes if a nucleotide site indicating the "Kyushu 199-type variant allele" associated with the DNA marker is detected in the detection step.SELECTED DRAWING: Figure 2

The invention discloses a molecular marker, a primer pair, a kit and a method for identifying the size character of watermelon seeds and application, and belongs to the technical field of molecular marker-assisted breeding. The invention provides a molecular marker (as shown in SEQ ID NO.1-2), a primer pair (as shown in SEQ ID NO.3-4) and a kit containing the primer pair for identifying the size character of watermelon seeds in order to identify the size character of the watermelon seeds more quickly and more accurately in the watermelon seedling stage and realize watermelon germplasm resource screening and molecular assisted selection breeding. According to the identification method provided by the invention, the to-be-detected watermelon genome DNA is extracted in the seedling stage, so that molecular marker-assisted selection can be performed on the watermelon seed size character, the operation is simple and convenient, the breeding accuracy and efficiency are improved, the variety breeding period is shortened, and the variety breeding process is accelerated.

The invention relates to a method for rapidly detecting the cell adhesiveness of probiotics and application of the method. The method comprises the following steps: adding the probiotics into adherent culture cells, incubating, discarding liquid, digesting and collecting the cells by pancreatin, extracting total DNA (Deoxyribonucleic Acid), performing qPCR (Quantitative Polymerase Chain Reaction) amplification, and calculating the adhesion quantity of the probiotics on a single cell according to a Ct value. The invention provides a method for rapidly detecting the cell adhesion rate of probiotics by using a qPCR (quantitative polymerase chain reaction) technology, which comprises the following steps of: extracting total genome DNA (deoxyribonucleic acid) after the probiotics are adhered to cells on the basis of the gene expression difference of prokaryotic and eukaryotic cells, and detecting the specific gene expression level of the probiotics and the cells by using the qPCR technology. The cell adhesion ability of the probiotics is evaluated by calculating the ratio of the gene expression quantities of the two, and the method has the characteristics of high efficiency, rapidness, high accuracy and the like.

The invention provides a method for simultaneously detecting three hematogenous parasites based on RAA-LFS and application of the method. The method comprises the following steps: firstly, screening target genes of genomic DNA of specific amplified toxoplasma gondii, babesia and plasmodium and primers of the target genes; a primer and probe combination for specifically amplifying the 18s rRNA-1 gene of the toxoplasma gondii, the 18s rRNA-2 gene of the babesia and the 18s rRNA-3 gene of the plasmodium is determined; rapid multiple detection is completed in a reaction system by utilizing an RAA constant-temperature amplification technology, and a detection result is intuitively judged by combining a lateral flow chromatography test strip, so that the detection efficiency is greatly improved, and the result can be obtained in a short time. The RAA-LFS detection method provided by the invention has remarkable advantages in application scenes such as epidemic disease prevention and control or blood screening.

The invention provides an isothermal amplification primer probe set for detecting Colletotrichum fucicola of Carya illinoensis, a rapid detection kit, a method for detecting Colletotrichum fucicola and application of the isothermal amplification primer probe set and the rapid detection kit, and belongs to the technical field of biological detection. Aiming at the limitation problems that the current apocarya colletotrichum gloeosporioides is difficult to identify, excessively depends on expensive instruments and technicians and the like, the apocarya colletotrichum gloeosporioides LFD-RPA detection technology research is developed, the apocarya colletotrichum gloeosporioides can be simply, conveniently and efficiently detected, the apocarya colletotrichum gloeosporioides LFD-RPA has no cross reaction with 22 representative strains of the colletotrichum gloeosporioides, the specificity is high, the detection time is the lowest detection limit is 10 pg of genome DNA, and the sensitivity is high. The method is suitable for port quarantine, scientific research institutions, agriculture and forestry management, farmer households and other departments to rapidly detect the diseases in real time on site.

Methods, systems, and software for estimating circulating tumor fraction are provided. A first plurality of nucleic acid sequences for a plurality of loci in genomic DNA from a solid tumor sample is obtained. A second plurality of nucleic acid sequences for a plurality of cell-free DNA fragments obtained from a liquid biopsy sample from the same subject is obtained. One or more somatic mutations is identified in the first plurality of nucleic acid sequences. A variant allele frequency (VAF) is determined for each somatic mutation based on a frequency of the respective somatic mutation in the liquid biopsy sample and a frequency of the corresponding wild type allele in the liquid biopsy sample, thereby determining a set of VAFs. An estimate of the circulating tumor fraction for the test subject is determined based on the set of VAFs for the one or more somatic mutations.

The invention discloses an indel molecular marker for identifying color and luster of citrus peel in a seedling stage and application of the indel molecular marker. The corresponding sites of the indel molecular marker of the citrus are positioned in chr7: 18, 398, 798-18, 399 and 289 intervals of a chromosome 7 of an SWO3.0 reference genome; the genome DNA of the citrus seedling stage plant is used as a template, the Indel molecular marker is amplified through PCR, the citrus seedling stage plant can be divided into a homozygous insertion type, a homozygous deletion type and a heterozygous type according to a PCR amplification result, and the three types are associated with the color of the citrus fruit for identifying the color of the citrus peel, so that the early screening of the seedling stage target traits of the citrus breeding population is realized, and the early screening of the seedling stage target traits of the citrus breeding population is realized. The breeding period is remarkably shortened, the breeding cost is reduced, and important technical support is provided for citrus fruit color improvement breeding.

The invention relates to a genome regulatory element analysis method based on deep learning. The method comprises the following steps: acquiring a genome DNA sequence and DNA shape information; on the basis of a shared hybrid neural network, transcription factor binding site features are extracted from a genome sequence, and binding specificity prediction is carried out aiming at the sequence level; aiming at the specificity of transcription factor binding, accurately positioning a transcription factor binding site on a nucleotide level by adopting a full convolutional neural network coupled with different models, and identifying a motif; for a chromosome three-dimensional genome, a chromatin ring is recognized in the three-dimensional whole genome based on a deep learning model, the relationship between the chromatin ring and regulatory elements in all cell lines is analyzed, and the chromatin ring represents cell type specificity and co-enrichment of transcription factor motifs among different cell lines and species. Compared with the prior art, the method has the advantages that the regulatory elements of the regulatory mechanism can be identified and analyzed by utilizing multiple information sources, and the accuracy of predicting the whole genome chromatin ring is effectively improved.

CRISPR-Cas systems have been engineered for various purposes, such as genomic DNA cleavage, base editing, epigenome editing, and genomic imaging. Although significant developments have been made, there still remains a need for new and useful CRISPR-Cas systems as powerful precise genome targeting tools. The invention disclosed herein comprises CRISPR-Cas based methods for high integration and expression efficiency of transgenes together with high post-transfection cell viability in eukaryotic cells.

The present application discloses a specific DNA fragment for sex identification of Pelteobagrus ussuriensis and a method for sex identification, specifically relating to the technical field of sex identification of Pelteobagrus ussuriensis. The above-mentioned identification method includes the following steps: Dissect 5 female and 5 male Pelteobagrus ussuriensis of a full-sib family to determine the physiological sex; respectively extract DNA from the muscles of female samples and male samples, construct a paired-end genomic DNA library with an insert fragment size of 300 bp, and perform high-throughput sequencing; based on the GWAS and Fst methods, identify that the sex chromosome of Pelteobagrus ussuriensis is chromosome 8, and then screen for Indels within the sex determination region on chromosome 8; screen a 601-bp male-specific DNA sequence and design and synthesize primers; use PCR amplification and agarose gel electrophoresis detection, and the result without specific bands is female, and the one with a 356-bp band is male. This scheme has the advantages of strong specificity, simple primer composition, and more intuitive identification of the sex of Pelteobagrus ussuriensis.

The invention discloses an engineering bacterium capable of efficiently degrading NP as well as a construction method and application of the engineering bacterium, and belongs to the technical field of wastewater treatment. The engineering bacterium is pseudomonas monteilii EBTRC-PM with an overexpressed ubiH gene, and the engineering bacterium is a pseudomonas monteilii EBTRC-PM The construction method comprises the following steps: designing a primer according to the genomic DNA of the pseudomonas monteilii EBTRC-PM, carrying out PCR to obtain a ubiH gene segment, carrying out homologous connection on the ubiH gene segment and a plasmid, replacing a T7 promoter of a reconstructed plasmid with a strong promoter P13, and finally transferring into the pseudomonas monteilii EBTRC-PM to obtain the engineering bacterium capable of efficiently inhibiting the toxicity in the NPEO degradation process, so that the NP degradation capability is greatly improved, and the engineering bacterium has the advantages that the construction is simple, and the construction cost is low. Good application prospects are realized in the field of wastewater treatment.

The invention discloses a tricholoma matsutake mushroom, which is classified and named as tricholoma matsutake mushroom 11, and is preserved in China Center for Type Culture Collection on March 24, 2025 with the preservation number of CCTCC NO: M 2025577. The molecular marker identification method comprises culture and collection of hyphae, extraction of genome DNA, DNA fingerprint PCR amplification and electrophoresis detection of PCR products. According to the method, the tricholoma matsutake mushroom 11 which inherits the advantage of high thermotype of two parents, is good in hypha temperature tolerance and high in vigor, is similar to tricholoma matsutake in sporocarp shape and is not opened is obtained by carrying out crossbreeding of the mushrooms and screening after mushroom cultivation and fruiting, and the tricholoma matsutake mushroom 11 has good development and application prospects.

The invention provides an isothermal amplification primer probe group, a rapid detection kit, a method and application for detecting Xanthomonas vesicans of Carya illinoensis, and belongs to the technical field of biological detection. Aiming at the limitation problems that the current carya illinoensis scab is difficult to identify, excessively depends on expensive instruments and technicians and the like, the research on the LFD-RPA detection technology of the carya illinoensis scab is developed, and the carya illinoensis scab can be simply, conveniently and efficiently detected; in addition, the kit has no cross reaction with 18 representative strains of other species and similar species (cladosporium) and other 14 genus in the genus of furazolospora, the specificity is strong, the lowest detection limit is 5 pg genome DNA, and the sensitivity is high. The method is suitable for port quarantine, scientific research institutions, agriculture and forestry management, farmer households and other departments to rapidly detect the diseases in real time on site.

The invention discloses a KASP molecular marker, a primer pair, a kit and a method for identifying sweet melon pericarp color traits and application, and belongs to the technical field of molecular marker-assisted breeding. The invention provides a KASP molecular marker (SEQ ID NO.1-2), a primer pair (SEQ ID NO.3-5) and a kit containing the primer pair for determining the pericarp color character of the sweet melon in order to identify the pericarp color character more quickly and more accurately in the seedling stage of the melon and realize screening of germplasm resources and molecular-assisted selection breeding of the germplasm resources, and provides the KASP molecular marker (SEQ ID NO.1-2) for determining the pericarp color character of the sweet melon, the primer pair (SEQ ID NO.3-5) and the kit containing the primer pair. According to the identification method provided by the invention, the to-be-detected muskmelon genome DNA is extracted in the seedling stage, so that molecular marker-assisted selection can be performed on the sweet melon peel color character, the operation is simple and convenient, the breeding accuracy and efficiency are improved, the variety breeding period is shortened, and the variety breeding process is accelerated.

The invention discloses an extraction method and application of high-molecular-weight turbellarian worm genome DNA, and belongs to the field of turbellarian worm genome extraction.The turbellarian worm genome DNA extraction is achieved by improving a lysate formula and removing ethylenediaminetetraacetic acid and increasing Mg < 2 + > on the basis of a conventional reagent in a classic Tris-HCl-phenol-chloroform method to inhibit activity of deoxyribonuclease II in a turbellarian worm body to inhibit degradation of genome DNA, and the high-molecular-weight turbellarian worm genome DNA is obtained. Meanwhile, ribonuclease A is added, RNA in cells can be degraded in the tissue cell digestion process, so that the step of two times of purification is omitted, the loss of DNA yield is reduced, experimental steps are simplified, especially the step of DNA purification is reduced, the problem of degradation in the extraction process of the turbellarian turbellarian worm genome DNA is solved, and the genome DNA yield (gt; 10 micrograms per turbellarian worm), and the turbellarian worm genome ultra-long sequencing or PacBio high-fidelity sequencing requirements are met.

The invention provides a PCR (polymerase chain reaction) method for rapidly screening effective donors for FMT (fibronectin) treatment of depression, which comprises the following steps: (1) collecting excrement samples of the donors, and extracting total microbial genome DNA (deoxyribonucleic acid) of the excrement samples; (2) designing six primer pairs according to a specific conserved sequence of a target strain; (3) taking the extracted donor excrement DNA as a PCR (Polymerase Chain Reaction) template, and adding a primer pair for amplification; (4) observing a PCR result through electrophoresis; and if each target band is positive in the PCR electrophoresis result of the donor sample, determining that the donor contains the target strain, and determining that the donor is an effective donor for FMT treatment of depression. The effective donor suitable for FMT treatment of depression is screened out by detecting whether the donor faeces contain three key probiotics closely related to depression symptom relieving or not, a basis is provided for microbial therapy of mental diseases such as depression, and important clinical application value is achieved.