138 results about "Microarray" patented technology

The invention provides a sika deer antler heavy whole genome SNP site combination, a probe, a chip and application thereof, and relates to the technical field of molecular breeding. The sika deer antler weight whole genome SNP locus combination comprises 6302 SNP loci, the number of the loci is sufficient, the loci can fully cover a main genetic area affecting deer antler weight, and the accuracy, stability and repeatability of genetic evaluation are effectively improved; the Northeast sika deer capture efficiency evaluation is carried out on a Northeast sika deer sample based on a probe designed on 6302 SNP loci, the capture proportion is kept at 98.60%-99.80%, the overall capture efficiency is high, the stability is good, and the Northeast sika deer capture efficiency evaluation method has good application value.

The invention relates to the technical field of molecular diagnosis and graphic processing, in particular to an electronic microarray chip dot matrix negative and positive judgment method based on an ISFET sensor. A chip hole site corresponds to an ISFET sensor, and after a hole bottom specific nucleic acid probe and a target sequence are hybridized and extended, the voltage of the sensor is changed. The method specifically comprises the following steps: data preprocessing: filtering median values of multi-frame electric signals of a hole site and taking a mean value; the dot matrix identification comprises the steps of threshold demarcation, Canny edge detection, circular Hough transform, optimal circle screening by Kdtree, array numbering and abnormal deletion processing to position a dot matrix; and calculating a result, extracting a signal difference value of the D1 stage and the D4 stage, evaluating numerical values of the positive quality control array and the to-be-detected sample array through a numerical value of the negative quality control array, and judging a final calculation result. According to the method, response changes are rapidly analyzed through image recognition, false detection and missing detection caused by numerical value differences in different arrays are reduced through multiple times of detection, and the detection accuracy is improved by selecting the most possible area through tickets.

The invention discloses a carbon-based microarray pressure sensor for pneumatic monitoring of an airfoil and a preparation method, belongs to the technical field of wind tunnel experimental equipment and advanced sensors, and aims to solve the problem of how to realize high-density, synchronous and accurate measurement of dynamic and static pressure of the airfoil surface on the premise of not damaging the airfoil structure. The sensor comprises a flexible substrate, a dynamic pressure sensitive element and a static pressure sensitive element, the flexible substrate is of a three-layer structure with gradient modulus; the dynamic pressure sensitive element is arranged on the flexible substrate and is used for sensing dynamic pressure; the static pressure sensitive element is arranged on the dynamic pressure sensitive element and is used for sensing static pressure; wherein the flexible substrate comprises an adhesion bottom layer, a middle buffer layer and a top supporting layer, the adhesion bottom layer is adhered to a wing surface, and bi-component microcapsules are dispersed in the adhesion bottom layer; the top supporting layer is provided with a micro-island bridge array structure; and the dynamic pressure sensitive element is fixedly supported on the micro-island bridge array.

The application provides a preparation method of a security label and the security label, and the preparation method comprises: sequentially forming a first security structure and a second security structure in a laminated arrangement on a surface of a substrate, forming the first security structure comprises forming a perovskite film on the surface of the substrate, the perovskite film can be irradiated by a first light beam to present a first preset pattern, and forming the second security structure comprises forming a structural color film on a side of the perovskite film away from the substrate, the structural color film can be irradiated by a second light beam to present a second preset pattern. Wherein, the structural color film is formed by irradiation light sequentially irradiating through a first mask plate and a second mask plate, a mask pattern of the first mask plate is the same as the second preset pattern, and the second mask plate is a first-pitch grating, so that the structural color film presents a microarray structure, based on the microarray structure, the structural color pattern and the fluorescent pattern can be selectively hidden and displayed, and then information encryption of the security structure is realized.

On an array surface (32) of a microlens array (31), lenses are arranged in a lattice shape. The lenses (30) have convex surfaces which are cylindrical through cross sections parallel to a lattice direction of the lenses (30) and orthogonal to the array surface (32). In each meridian on each cross section of the lenses (30) parallel to the lattice direction, the sag is corrected so that the inclination of the skirt becomes large. In other modes, the lenses have concave surfaces which are cylindrical through cross sections parallel to a lattice direction of the lenses and orthogonal to the array surface. In each meridian on each cross section of the lenses parallel to the lattice direction, the sag is corrected so that the inclination of the edge becomes large.

A removable cartridge to be used in a system for extracting and detecting nucleic acids from heterogeneous samples includes a plurality of reservoirs defining at least a first wash buffer reservoir for holding a first wash buffer and a microfluidic assembly configured to attach to the plurality of reservoirs. The microfluidic assembly includes at least one sample reservoir and a nucleic acid extraction matrix in fluid communication to an automated sample preparation (ASP) reservoir through a first flow channel defined by the microfluidic assembly. An assay chamber is in fluid communication with a third flow channel and with the waste reservoir through a fourth flow channel such that a labeled nucleic acid-containing sample flows through the assay chamber and then to the waste reservoir, wherein vibration-driven mixing agitates fluids while present in the assay chamber. Finally, a nucleic acid-detecting microarray module is positioned in the assay chamber.

Dispensable formulations, microneedles, microarray patches (MAPs), and systems that can facilitate simple, substantially pain-free, and consistent delivery of a wide range of active pharmaceutical ingredients (APIs) and doses, as well as methods of manufacturing and using the same are described herein.

This invention belongs to the field of biomedical engineering technology, providing a method for preparing modular bio-ink for cell spheres and its application in the field of myocardial repair therapy. First, a method for preparing stem cell spheres based on microarrays is described. By introducing a protein / polyphenol composite coating onto the cell spheres, a multifunctional armor is created, providing immune protection and immunomodulatory therapy against the host's immune system to reverse the hostile microenvironment and promote the healing process of infarcted myocardial tissue. Second, using microfluidic droplet control technology, gel microspheres loaded with bioactive substances are prepared from biocompatible biomaterials. These microspheres are then densely packed and coated to form cell sphere particle gels. These gels can be used for disease treatment via direct injection or bio-3D printing, showing promising application prospects.

A microarray patch (1) is disclosed comprising a substrate (2) and an array of microneedles (3) arranged on the substrate (2). The substrate (2) is configured to indicate detection of moisture. The substrate (2) may comprise a dye that changes color upon contact with moisture. The color change of the substrate is configured to coincide with delivery of a substance through or from the microneedle or capture of the substance into the microneedle. The dye may be configured to produce a gradual color change as a function of the degree of wetting. The substrate (2) may be insoluble in water and hydrophobic. A method of manufacturing a microarray patch (1) is also disclosed.

This invention discloses a droplet microarray chip based on molecular logic operations and its construction method, belonging to the field of biochemical analysis and detection technology. It includes a substrate layer, a functional layer on the substrate layer, and molecular logic circuitry. The functional layer is a superhydrophilic-superhydrophobic patterned porous polymer film prepared in situ on the substrate using digital light processing technology, followed by PFOC acylation. The superhydrophilic-superhydrophobic patterned porous polymer film contains arrayed superhydrophilic microreaction units, separated by superhydrophobic polymer regions. Each superhydrophilic microreaction unit encapsulates an independent microdroplet, and the molecular logic circuitry is encapsulated within each independent microdroplet. This invention, through the combination of droplet microarray chip and molecular logic circuitry, achieves systematic improvements in multi-enzyme synergistic detection capabilities, logic computation complexity, spatial isolation control, high-throughput analysis, and adaptation to biological environments.

The invention provides a corneal limbus stem cell in-situ repair microneedle array patch and a preparation method and application thereof.The corneal limbus stem cell in-situ repair microneedle array patch comprises a biocompatible backing layer and a microarray layer arranged on the biocompatible backing layer, microneedles of the microarray layer are of a conical structure, the height is 100-300 micrometers, the vertex angle is smaller than 30 degrees, and the thickness of the microneedles of the microarray layer is 10-30 micrometers. And a nano-scale porous structure for loading cornea stem cell suspension is arranged in the cornea stem cell support. Through precise geometric design, biomimetic material selection and spatial arrangement optimization, in-situ delivery, microenvironment activation and tissue fusion in a corneal limbus area are realized under the condition of not depending on in-vitro amplification and surgical suture, and the method becomes an important direction for promoting LSCD minimally invasive treatment strategy innovation.

A microarray manufacturing apparatus according to an embodiment of the present invention comprises a microarray tip manufacturing apparatus and a microarray base manufacturing apparatus. The tip manufacturing apparatus includes: a dispensing unit that dispenses a tip composition including an active ingredient into an engraved portion of a supplied mold; and a first filling unit that simultaneously performs filling, in which even fine structures in the engraved portion of the mold are filled with the tip composition dispensed into the engraved portion of the mold, and drying of the filled composition. The base manufacturing apparatus includes: a dispensing unit that receives the mold, in which the tip composition has been filled and dried, and dispenses a base composition onto the tip composition filled and dried inside the engraved portion of the mold; and a second filling unit that simultaneously performs filling and drying of the base composition dispensed into the engraved portion of the mold.

This invention discloses a system for assessing the activity of inflammatory bowel disease (IBD) based on multimodal endoscopic feature fusion, relating to the field of medical information processing. The system includes: a multimodal data receiving module for receiving dynamic sequence data from a first EUS microarray multimodal endoscopy; an image preprocessing module for noise reduction and determining first effective white light observation images and first effective ultrasound observation images; a white light surface feature extraction module for extracting mucosal surface features using a white light surface feature extraction model to determine the first white light endoscopic mucosal features; an ultrasound transmural feature extraction module for structural identification and measurement using an ultrasound transmural feature extraction model to determine the first ultrasound-guided mucosal features; and a multimodal endoscopic IBD activity assessment module for determining IBD activity based on multimodal endoscopic features using a multimodal dynamic endoscopic IBD activity assessment model. Compared to existing technologies, this invention overcomes the limitations of previous disease assessments based on single-modality, static images, improving the accuracy of IBD activity assessment results.

The invention discloses a functionalized microarray pore plate, a preparation method thereof and application of the functionalized microarray pore plate in membrane protein ligand screening, and belongs to the technical field of biological medicine analysis. Amino and vinyl sulfuryl are covalently modified on the surface of the microarray pore plate to introduce an active site which can be specifically covalently bound with a membrane protein or a tag group, so that directional fixation of a target membrane protein on the surface of the microarray pore plate is realized, and the functionalized microarray pore plate is obtained. A functional microarray pore plate and a specific fluorescent probe are combined to screen a membrane protein ligand, the specific fluorescent probe is combined with a receptor active site of a fixed protein, and a detectable fluorescence signal change is generated by utilizing a competitive replacement mechanism of the probe and a candidate compound at the active site; the method is used for in-vitro screening and discovery of potential membrane protein ligands.

The invention provides a tumor in-situ pathology detection system based on an endoscopic multi-mode probe. The tumor in-situ pathology detection system comprises a main controller, an SC laser, a DUV laser, a comb filter, a polarizer, a microarray lens, a multiplexing beam combiner, a conjugate coupling module, an endoscopic optical fiber bundle, a FAST optical fiber bundle, an SC spectrograph and a DUV spectrograph. Aiming at G14-level bronchial navigation surgical robot in-situ pathology detection, the invention provides a detection system based on an endoscopic multi-mode optical probe, FAST hyperspectral image spectrum integrated detection of SC, DUVRS, DUVLIF and mIHC can be realized, a multi-mode hyperspectral fusion image reflecting spatial distribution of histochemical components of a diagnosis point and corresponding spectral feature data are output, and the detection system is used for detecting the in-situ pathology of the G14-level bronchial navigation surgical robot. And real-time, accurate and multi-mode data or information is provided for diagnosis of doctors.

The invention discloses a micro-fluidic chip based on a polylysine and gold nanorod assembly array and a preparation method of the micro-fluidic chip. The micro-fluidic chip comprises a pressing cap top cover layer, a PMMA runner layer and a glass substrate which are sequentially arranged from top to bottom, a nucleic acid extraction runner is arranged in the PMMA runner layer, and the inner wall of the PMMA runner layer is covalently modified with a polylysine functional layer and a vertical-oriented periodic gold nanorod array in sequence; when a nucleic acid solution is injected into the nucleic acid extraction runner, nucleic acid is adsorbed under the synergistic effect of the cationic characteristic of polylysine and the high specific surface area of the gold nanorod; when the eluent is injected, the polylysine releases nucleic acid under the environment of specific ionic strength and pH, and the photothermal effect of the gold nanorod can assist in accelerating desorption. According to the micro-fluidic chip prepared by the invention, nucleic acid extraction is directly realized through the nucleic acid extraction runner, magnetic beads or microarrays are not needed, the cost is low, fluid control is simple, the adsorption efficiency is enhanced by the gold nanorod array, and the micro-fluidic chip is suitable for rapid and high-sensitivity nucleic acid detection.

This invention discloses an adaptive compensation control method for uneven settlement in soft strata, belonging to the field of settlement control in geotechnical engineering. The method involves collecting distributed micro-strain field and pore water pressure gradient data at the bottom of the foundation in the soft strata and inputting them into a stratum rheological evolution prediction model. Multi-scale spatiotemporal features are extracted using a spatiotemporal attention coding module to generate a stratum creep trend vector. The equivalent modulus correction coefficient is calculated using a virtual stiffness reconstruction algorithm to generate a dynamic stress wave excitation parameter set. Coherent stress beams are emitted using microarray actuators embedded in the bearing layer interface, and a dynamic stress-locking zone is constructed through constructive interference to form a virtual rigid support. The excitation parameters are corrected through a dual-loop adaptive feedback control mechanism combined with a heuristic optimization algorithm until the micro-strain field returns to uniformity. Simultaneously, energy dissipation monitoring and safety mechanisms are added to mitigate the risk of soil liquefaction. This invention achieves active suppression and adaptive compensation for uneven settlement in soft strata, improving the accuracy of settlement control.

The invention relates to a kinetic analysis system and method for a fluorescent microarray. The system comprises a data input module, an initialization processing module, an inter-frame processing module, a kinetic analysis module and a result output and interaction module. The method comprises the steps of time sequence image acquisition, initialization processing and theoretical grid model establishment, frame-by-frame processing including image registration and probe detection, Ct value calculation through kinetic analysis, result visualization display and the like. According to the method, high-precision grid initialization is achieved through Hough transform and clustering analysis, the RANSAC algorithm is used for accurate probe positioning, a Ct value analysis model in the qPCR field is introduced for kinetic analysis, and an interactive visual interface is designed. The system has high-throughput treatment capacity, supports analysis of as many as 1000 probes, improves the accuracy and efficiency of detection, and is suitable for the fields of high-throughput molecular diagnosis and scientific research.

The application discloses a kind of absolute quantitative detection method of child respiratory tract multi-pathogen based on multi-target ladder microarray and exogenous internal reference correction.The method sets up independent micro-reaction unit to different pathogen, adds fixed amount of exogenous internal reference nucleic acid, and calculates template number by positive unit proportion combined with Poisson distribution model, then corrects the result by using the theoretical addition amount of exogenous internal reference, so as to realize parallel detection and absolute quantification of pathogen such as bocavirus, adenovirus, mycobacterium tuberculosis and mycoplasma pneumoniae without standard curve and special digital PCR equipment.The application has the advantages of high sensitivity, strong anti-interference ability, suitable for mixed infection sample analysis and clinical application, etc.

This invention discloses methods and composition to form biodegradable polymer implant arrays in the live tissue. Artificial cavities are created in the live tissue by using laser ablation, oscillating needle, microneedle array and other methods. The cavities are then filled with biodegradable polymer solution. The solvent in the polymer solution is dissipated in the tissue to form a biodegradable polymer implant in artificial cavities. The cavities and implants formed are arranged to form of an array of implants. The biodegradable polymer in the cavity can also be loaded with drug to form biodegradable drug delivery array in the live tissue.

This invention relates to the field of gastric cancer organoid microarray technology, and more particularly to a gastric cancer organoid microarray culture medium and its applications. The invention provides a novel culture medium adapted for gastric cancer organoid microarray culture. The medium components include basal medium, penicillin / streptomycin dual antibiotics, antibacterial agents, hydroxyethylpiperazine ethanesulfonic acid, B-27 additive, Glutamax culture medium additive, R-vertebral protein 1, scutellarin B, and tetramethylpyrazine, among other components. This invention solves key problems in existing technologies such as slow growth, morphological instability, poor heterogeneity adaptation, and low long-term culture activity of gastric cancer organoids, providing an ideal experimental model for high-throughput screening and precision medicine applications on microarrays.

The application provides an endoscopic multi-mode probe-based tumor in-situ pathological detection system, which comprises a main controller, an SC laser, a DUV laser, a comb filter, a polarizer, a microarray lens head, a multiplexing beam combiner, a conjugate coupling module, an endoscopic optical fiber bundle, a FAST optical fiber bundle, an SC spectrometer and a DUV spectrometer. The application is aimed at G14-grade bronchus navigation surgery robot in-situ pathological detection, and proposes an endoscopic multi-mode optical probe-based detection system, which can realize SC, DUVRS, DUVLIF and mIHC FAST hyperspectral image spectrum integration detection, and output multi-modal hyperspectral fusion images reflecting the spatial distribution of the chemical components of the diagnosis point tissues and corresponding spectral characteristic data, so as to provide real-time, accurate and multi-modal data or information for doctors to diagnose.

The disclosure describes apparatus and methods for multiplexed amplification and detection of nucleic acid targets in a sample. Embodiments of the present disclosure include a mechanical system configured to provide loading, vertical positioning and clamping of a chip; a thermal control system configured to maintain distinct temperatures of the chip, and an optical fluorescence imaging system.

In some embodiments, tissue microarray (TMA) core images are used to train a deep learning network that can then be deployed to computer inferences regarding whole tissue section (WTS) images (WSIs). Preprocessing aligns paired serial core images from differently stained core sections with their associated metadata and H-scores (or other label data obtained from evaluating one of the paired core sections). In some embodiment, a self-supervised learning (SSL) pre-trained encoder is used to generate patch-level embeddings from TMA core images associated with corresponding labels that are then used to train an attention-based deep learning network to generate inferences. These and other aspects of the present disclosure are more fully detailed herein.

This invention discloses a single-cell microarray-based kit and method for in situ quantitative detection of receptor-binding allergen-specific IgE. The kit includes: a single-cell microcavity array chip, an antigen barcode chip, a plastic clamp, IgE standards, fluorescently modified IgE detection antibodies, and auxiliary reagents. During detection, basophil single cells are loaded into the microcavity array chip, lysis buffer is added, and the cells are clamped together with the antigen barcode chip. Low-temperature incubation allows the allergen-specific IgE released from cell lysis to be captured in situ by the antigen on the chip. A sandwich immunoassay is then performed using a fluorescently labeled antibody, and absolute quantification is achieved using a standard curve. This invention is the first to achieve high-throughput, high-sensitivity, multi-component parallel in situ quantitative detection of basophil membrane receptor-binding sIgE at the single-cell level. It can directly reflect the functional sensitization state of effector cells and has advantages such as simple operation, low sample requirement, and no need for complex valve devices.

The present invention relates to a microarray-based large-area spatial transcriptomic analysis method and, specifically, to: a microarray surface treatment method for spatial transcriptomic analysis, the method comprising a step of attaching a poly-T nucleotide sequence by treating the surface of a microarray with a terminal transferase; a microarray for spatial transcriptomic analysis, the microarray being surface-treated by the method; and a sequencing-based large-area spatial transcriptomic analysis method using the surface-treated microarray for spatial transcriptomic analysis.

The invention relates to the technical field of DNA microarrays, and discloses a preparation method and application of a DNA nano array. The preparation method of the DNA nano array comprises the following steps: step 1, acquiring DNA ink, and then transferring the DNA ink to the edge of a substrate material; step 2, moving a flexible platform to enable a probe to be in contact with the DNA ink, then dropping the probe at a first preset position of the substrate material and then lifting the probe to obtain a first DNA nanodot; and 3, changing the target preset position of the substrate material, and repeating the step 2 to obtain the target DNA nanodot. By adopting the method disclosed by the invention, the DNA nano array of which the average diameter is less than or equal to 500 nm, the distance between adjacent points is less than or equal to 2000 nm and the average height is less than or equal to 50 nm can be prepared, the prepared DNA nano array has the characteristics of high density and high flux, meanwhile, the preparation process is simple, the cost is relatively low, and no other toxic chemical reagents are introduced.

According to the application of the plasma exosome circRNA to the biomarker for early diagnosis of the lung adenocarcinoma, the plasma exosome marker circRNA is annular hsacirc0033861, hsacirc0043273 and hsacirc0011959, and the plasma exosome circRNA can be used as a biomarker for early diagnosis of the lung adenocarcinoma. The method comprises the following steps: firstly, constructing a circRNA / lncRNA-miRNA-mRNA regulation and control network in the plasma exosome on the basis of ceRNA microarray data and miRNA sequencing data of the exosome of a lung adenocarcinoma patient; according to the present invention, the lung adenocarcinoma patient specific plasma exosome with significant difference is screened by using a bioinformatics method so as to be adopted as the diagnosis marker, verification is performed in the lung adenocarcinoma patient through the reverse transcription real-time fluorescence quantitative polymerase chain reaction (RT-qPCR), and the hsacirc0033861, the hsacirc0043273 and the hsacirc0011959 are finally screened, and can be used as the lung adenocarcinoma early diagnosis marker.

This invention discloses an RPA-T7-CRISPR-based absolute quantification chip for ctDNA. The system employs a microarray digital partitioning structure as the reaction carrier, integrating an RPA isothermal amplification system, a T7 transcription amplification system, and a CRISPR / Cas13a specific recognition system within multiple independent reaction units. After the sample enters the chip, sample allocation, target nucleic acid amplification, transcription amplification, fluorescent reporter molecule cleavage, and signal reading are completed in a closed chip flow path. Then, based on fluorescence microscopy observation, the number of positive reaction units is counted, and their percentage of the absolute copy number of ctDNA in the sample is calculated. This chip, with its digital microarray partitioning and closed flow path design, integrates nucleic acid isothermal amplification, transcription amplification, specific recognition, and fluorescence readout, achieving rapid and highly sensitive absolute quantification of low-abundance ctDNA.

The invention discloses preparation of a low-background-leakage microarray detection system for catalyzing a hairpin self-assembly reaction in combination with time-resolved resonance energy transfer and application of the microarray detection system in esophageal cancer diagnosis, and belongs to the technical field of biomedical detection. The probe combination provided by the invention comprises a probe pair for detecting miRNA-21, and the nucleotide sequences of the probe pair are respectively SEQ ID NO.1 and SEQ ID NO.2; the nucleotide sequences of the probe pair for detecting the miRNA-25 are respectively SEQ ID NO.3 and SEQ ID NO.4; and / or a probe pair for detecting the miRNA-93, and the nucleotide sequences of the probe pair are respectively SEQ ID NO.5 and SEQ ID NO.6. TMGA is added in a reaction system, the thermal stability of the probe H1 / H2 is enhanced through combination of positive ions of TMGA and DNA small grooves, so that background leakage caused by respiration is inhibited, a time-resolved fluorescence technology is adopted, and serum short-life autofluorescence is avoided by delaying measurement time.