369 results about "Microarray" patented technology

The invention relates to the technical field of medical treatment, and particularly discloses a minimally invasive implantable brain-computer interface intracranial multi-mode signal monitoring system, which comprises a flexible probe, a data acquisition module, a data return module and a data analysis module, the flexible probe is embedded into the high-precision microarray electrode and the sensor to collect neural signals in real time; the data acquisition module is used for synchronously acquiring electroencephalogram signals and intracranial pressure of specific parts of the brain; the data return module manages the collected data; and the data analysis module analyzes the electroencephalogram signal and the intracranial pressure data. According to the minimally invasive implantable brain-computer interface intracranial multi-mode signal monitoring system provided by the invention, lumbar puncture enters a cerebrospinal fluid space and is sent to a target spot, so that the infection risk can be effectively reduced, the limitation on the action of a patient is reduced, and the system has the characteristics of real-time performance, directness and accuracy; the method has very important significance and value for evaluating brain injury and brain functional lesion of a patient, prognosis judgment and the like.

The invention discloses a temperature correction method of a chloride ion sensor, which comprises the following steps of: S1, arranging a micro array type chloride ion sensor of a pre-embedded concrete protective layer in an array mode, and determining an acquisition scheme; s2, carrying out anomaly detection on a MnO2 reference electrode in the micro array chloride ion sensor; s3, correcting an acquired potential value of the micro array type chloride ion sensor; s4, according to the Nernst potential-concentration logarithm linear relation, determining various coefficients of the standard equation under the control temperature in the concrete; and S5, determining a temperature compensation coefficient according to the Nernst potential-concentration logarithm linear relationship. And S6, calculating the concentration distribution condition of chloride ions at different depths in the concrete by using the sensor potential after temperature compensation. According to the invention, the influence of temperature on the measurement result of the chloride ion sensor can be effectively compensated, the potential abnormal interference in the measurement process of the sensor is effectively overcome, and the precision and reliability of the sensor are improved.

Microarray applicator (10000) including a microarray carrier (8000), which is retained within a housing (2000) by virtue of microarray carrier (8100) being engaged withholder (2400). Microarray patch (8200) and flexible backing (8220) are positioned such that the central portion (8210) has microneedles (8211) protrude in the direction opposite from the bottom (7200) of plunger (7000). Plunger (7000) is in a first position, having first notch (7310) engaged with ridge (2320). After activation, wherein plunger (7000) is in a second position such that second notch (7320) is engaged with ridge (2320). Here, bottom (7200) of plunger (7000) extends below first major surface (2100) of housing (2000) and has ejected microarray patch (8200).

The invention belongs to the technical field of biological detection, particularly relates to a gold nano pumpkin array, and relates to an SERS (Surface Enhanced Raman Scattering) sensor of a gastric precancerous lesion biomarker as well as a preparation method and application thereof. The preparation method of the gold nano pumpkin arrays (Au NPAs) comprises the steps that a PS single-layer template is obtained through a gas-liquid interface self-assembly technology, the PS single-layer template is sequentially subjected to plasma etching technology treatment, annealing treatment and ion sputtering treatment, and the gold nano pumpkin arrays (Au NPAs) are obtained. The SERS sensor and the SERS microarray chip constructed on the basis of the gold nano pumpkin array show excellent chemical stability, high molecular specificity and excellent reproducibility, ultra-trace detection of gastric cancer precancerous lesion biomarkers miR-196b and miR-221 is realized, and technical support is provided for early discovery, timely intervention and treatment of GC (Gas Chromatography).

The invention discloses sand grains with a water storage structure and a preparation method and application thereof. The method comprises the steps that S1, sand grains with the grain size ranging from 0.1 mm to 1 mm are selected, the grain size distribution meets the condition that D50 is equal to 0.3 mm, clay impurities are screened out, and the sand grains are placed on a sand grain base plate (1) after being cleaned and dried; s2, nano silicate powder is uniformly sprayed on the surfaces of the sand grains through an electrostatic spraying technology, so that a silicate glass layer (5) is formed on the surfaces of the sand grains; s3, a microarray lens (3) is used for dividing a laser source into multiple laser beams, each laser beam irradiates the silicate glass layer (5) on each sand grain in a one-to-one mode, silicate powder and the surface layers of the sand grains are co-melted, and then an amorphous net-shaped structure is formed; s4, argon is used for spraying the amorphous net-shaped structure so that the amorphous net-shaped structure can be rapidly cooled, and then a porous heterojunction structure is formed on the surface of the sand grains; and S5, scanning the silicate glass layer (5) by using the laser again, so that the part of the silicate glass layer (5) is molten again, silicate is decomposed to generate gas, and cooling and curing are performed after bubbles are formed in the molten glass. According to the invention, the sand grains have a certain water storage characteristic.

The invention belongs to the technical field of electrodes, and discloses a preparation method of an anti-scaling cathode with a micro-nano layered structure. The method comprises the following steps: preparing a metal microarray; preparing a micro-nano layered structure; constructing an alkali-resistant protective layer on the micro-nano layered surface; and pouring the inert protective shell. The cathode obtained by the preparation method can utilize generated H2 microbubbles to migrate along a hydrophobic surface and gather into a bubble layer, so that the surface of the cathode is prevented from being in contact with H2O, Ca < 2 + > and HCO3 <->. Therefore, only the nano active site is used as an anchor point of the scale crystal. After the scale crystals are stacked into a chain shape, the stability between the scale crystals and the anchor points becomes weaker and weaker. And meanwhile, H2 is continuously injected to enable the bubble layer to grow into large bubbles, and the large bubbles escape from the surface of the cathode under the action of buoyancy and break when moving to the liquid level. Vibration generated by bubble breakage causes automatic falling of a scale crystal chain which is not stably erected on an active point location. According to the invention, the problem of cathode scaling is fundamentally solved, and the method has huge application potential in wide fields.

The invention discloses a fluorescent anti-counterfeiting-encryption method for constructing a perovskite quantum dot array based on in-situ synthesis of a solid-phase photoetching method. The preparation method comprises the following steps: dissolving cesium perfluoroglutarate, lead oleate and hexabromocyclododecane in N, N-dimethylformamide, adding polymethyl methacrylate, heating and dissolving to obtain a mixed precursor solution, coating a glass substrate with the mixed precursor solution to obtain a precursor film, drying to remove N, N-dimethylformamide, and finally covering the precursor film with a pattern chromium mask plate to obtain the chromium-coated glass. And irradiating under a 254 nm ultraviolet lamp to obtain the CsPbBr3 fluorescent anti-counterfeiting array. According to the method, the perovskite nanocrystal fluorescent microarray is prepared in situ based on a photo-induced dehalogenation direct photoetching method, under the action of a mask plate, halogenated hydrocarbon is induced to dehalogenate through 254 nm ultraviolet light to generate halide ions and drive generation of perovskite nanocrystals, and perovskite nanocrystal fluorescent microarrays with different patterns can be obtained by adjusting the patterns of the mask plate. Developing, etching and other operations are not needed, and the method is very simple, convenient, efficient and low in cost.

The invention relates to a method for producing nano-arrays and micro-arrays. The method described herein provides a method of generating an array of spatially separated proteins. The method relies on covalently attaching each of a plurality of proteins to a structured nucleic acid particle (SNAP) and attaching the SNAP to a solid support.

The invention discloses a method for external field on-line calibration of a micro-array MEMS gyroscope. The method comprises the following steps: electrifying and initializing a micro-array inertial sensor; the output of the micro array MEMS gyroscope is converted into three-axis output of a carrier coordinate system; in combination with a maneuvering target tracking theory, establishing a Kalman filter for angular rate tracking, and estimating the angular velocity rate of each axis at each moment; establishing an adaptive Kalman filter for random drift error estimation by using the angular rate of each axis at each moment obtained by estimation, and respectively performing random drift error and deterministic error compensation on the xyz axis; taking the estimated angular rate of the random drift error compensated at each moment as a reference, establishing a deterministic error estimation Kalman filter, and performing compensation; twelve error parameters at the moment are obtained through estimation, and after the random drift error and the deterministic error of the current sampling angular rate are compensated, the final calibration angular rate at the current moment is obtained.

The invention provides improvements in the design of microneedles, microneedle arrays, microneedle patches or microarray patches. The invention combines a microneedle with a sensor for detecting physical parameters which relate to the receipt of fluid in the microneedle from a patient and a circuit in communication with the sensor for processing sensor data. It may be applied to the skin as a patch containing an array of microneedles to diagnose and / or monitor the health of a patient through contact between the microneedles which penetrate the epidermis to extract fluid from the patient.

The invention discloses an inflammatory bowel disease activity evaluation system based on multi-modal endoscopic feature fusion, and relates to the field of medical information processing. The system comprises a multi-modal data receiving module used for receiving first EUS microarray multi-modal endoscope dynamic sequence data; the image preprocessing module is used for carrying out noise reduction processing and determining a first white light effective observation image and a first ultrasonic effective observation image; the white light surface feature extraction module is used for extracting mucous membrane surface features by using a white light surface feature extraction model to determine mucous membrane features under a first white light endoscope; the ultrasonic transmural feature extraction module is used for performing structure identification and measurement by using an ultrasonic transmural feature extraction model to determine first ultrasonic lower mucous membrane features; and the multi-modal endoscope IBD activity evaluation module is used for determining the IBD activity based on the multi-modal endoscope characteristics by using the multi-modal dynamic endoscope IBD activity evaluation model. Compared with the prior art, the method has the advantages that the limitation of disease assessment based on single-mode and static pictures in the past is broken through, and the accuracy of IBD activity assessment results is improved.

The invention discloses a low-temperature antifouling and directional icing microarray structure surface and a preparation method thereof, a tile-covering-shaped arrangement structure with an asymmetric groove structure on the surface is prepared, and a substrate of the structure is a wavy base material. In a low-temperature environment, due to the existence of the tile-shaped arrangement structure, water molecules are more easily gathered and nucleated at the edge of the tile-shaped arrangement structure. When the ice core forms an ice bar, the airflow around the tile-shaped arrangement structure begins to flow to the ice core, and a higher atmospheric pressure gradient is generated locally, so that more water vapor flows to ice on the edge of the tile-shaped arrangement structure. And meanwhile, the asymmetric groove structure divides the air flow into smaller intervals, so that the air in the air flow is more easily pushed by the air pressure difference at the two ends of the groove to flow to the edge of the tile-shaped arrangement structure, and the directional icing function is achieved. The workpiece prepared on the basis of the characteristics can be prevented from being covered by ice crystals in a low-temperature environment, and the surface subjected to spraying treatment has relatively low surface energy and low adhesion, so that the surface has a good antifouling effect.

Disclosed in the present invention are a solid electrolyte membrane, and a preparation method therefor and the use thereof. The solid electrolyte membrane comprises a microarray support membrane and a solid electrolyte material loaded on the microarray support membrane, wherein the microarray support membrane is provided with an array-shaped micropore structure, the duty ratio of the micropore structure being 50%-99%, and the diameter of micropores in the micropore structure being 1-500 μm. The solid electrolyte membrane of the present invention has the effect of homogenizing current density, and is characterized by being ultrathin, highly flexible, capable of homogenizing current density, etc.

The invention belongs to the technical field of medicine, and particularly relates to application of a small molecule compound R406 in preparation of a product for preventing and treating Parkinson's disease, screening based on small molecules and microarrays is carried out, the compound R406 can reduce the alpha-syn protein level in an autophagy-dependent mode, and in addition, the compound R406 can be used for preparing a product for preventing and treating Parkinson's disease. The R406 also reduces the level of alpha-syn phosphorylated serine 129 in a fiber-induced cell model pre-formed by alpha-syn, and prevents neuronal degeneration. In addition, experiments confirm that the R406 can relieve PD-related disease phenotypes in a human alpha-syn preformed fiber-induced cell model and a PD patient-derived organ-like model, and a new thought is provided for treatment of the Parkinson's disease.

The invention provides a low-light-level enhancement camera imaging enhancement method and system, and the method comprises the steps: obtaining a target video shot by a microarray fly's-eye lens camera, carrying out the frame-by-frame splitting processing of the target video, so as to obtain a plurality of target video frames, and carrying out the preprocessing of the corresponding target video frames, so as to obtain a plurality of target images; establishing an initial filter, performing updating processing on the initial filter to obtain an updated filter, and performing adaptive filtering processing on the target image based on the updated filter to obtain a filtered image; identifying a noise level of the filtered image, and determining a low-light image frame based on the noise level; gaussian filtering processing and detail enhancement are carried out on the low-light image frame; according to the method, the corresponding low-light-level image is determined according to the noise level of the image, enhancement processing is carried out on the low-light-level image, the calculation complexity is remarkably reduced, and super-resolution enhancement of the low-light-level image is achieved.

The invention provides a method for machining a straight micro through hole in glass through ultrafast laser and a machining system. The method comprises the following steps that three glass workpieces are bonded into a sandwich type workpiece through an optical cement technology; the Bessel beam is focused on the sandwich type workpiece by adjusting the length of the Bessel beam; ultrafast laser irradiates the sandwich type workpiece, so that the material structure of the inner area of the glass is converted into a silicon-oxygen three-membered ring or a silicon-oxygen four-membered ring from a silicon-oxygen six-membered ring, and the sandwich type workpiece with a vertically-through modified microarray area inside is obtained after processing; the sandwich type workpiece after laser processing is separated through the optical cement removing technology, and only the target workpiece in the middle is reserved; putting a target workpiece into an etching solution, introducing ultrasonic oscillation for etching, and corroding a silicon-oxygen three-membered ring or a silicon-oxygen four-membered ring in an internal modified microarray region by an etching agent to form a micro through hole; and cleaning the etched target workpiece. The appearance of a horn mouth can be avoided.

The invention discloses a microorganism-intestine-joint axis multi-organ chip and a preparation method thereof, and belongs to the field of biomedical engineering.The preparation method comprises the following specific steps that firstly, an upper-layer chip male mold, a middle-layer chip male mold and a lower-layer chip male mold of the multi-organ chip are prepared through the high-precision 3D printing technology; iI, processing the silicon wafer with the cylindrical microarray through photoetching to prepare a porous membrane; iII, preparing an upper-layer chip, a middle-layer chip and a lower-layer chip; iV, assembling each group of prepared chips; according to the invention, human cells or organoids can be used for experiments, the experiment result has good correlation with human beings, and there is no species difference between animal experiments and human beings; the experiment process can be obviously accelerated, and the experiment time is shortened; according to the method, the experimental cost can be greatly saved, and the dispute of experimental ethics does not exist, so that the method can be expanded to large-scale standardized processing production so as to carry out high-throughput and large-scale experimental research.

A microarray patch (1) comprising a baseplate (2) and an array of microneedles (3) arranged on the baseplate (2) is disclosed. The baseplate is (2) configured to indicate the detection of moisture. The baseplate (2) may comprise a dye that changes colour on contact with moisture. The colour change of the baseplate is configured to align with the delivery of a substance through or from the microneedle, or the capture of a substance into the microneedle. The dye may be configured to yield gradual colour change according to the degree of moisturisation. The baseplate (2) may be insoluble with water and hydrophobic. Also disclosed is a method of manufacturing the microarray patch (1).

The invention provides a sika deer antler heavy whole genome SNP site combination, a probe, a chip and application thereof, and relates to the technical field of molecular breeding. The sika deer antler weight whole genome SNP locus combination comprises 6302 SNP loci, the number of the loci is sufficient, the loci can fully cover a main genetic area affecting deer antler weight, and the accuracy, stability and repeatability of genetic evaluation are effectively improved; the Northeast sika deer capture efficiency evaluation is carried out on a Northeast sika deer sample based on a probe designed on 6302 SNP loci, the capture proportion is kept at 98.60%-99.80%, the overall capture efficiency is high, the stability is good, and the Northeast sika deer capture efficiency evaluation method has good application value.

The disclosure discloses a chemical synthesis method and an application of V. cholerae serotype O100 O-antigen oligosaccharides, belonging to the field of chemical technologies. The disclosure uses three monosaccharide building blocks and five carboxylic acid derivatives to synthesize five oligosaccharide fragments of V. cholerae serotype O100 O-antigen under the action of the solvent effect, temperature effect, neighboring group participation effect, etc., through orthogonal protection, selective assembly and amide coupling. The absolute configurations and immunological effects of 3,5-dihydroxyhexanoyl in the O-antigen trisaccharide are illustrated by the synthesized oligosaccharide fragments in combination with the NMR analysis and glycan microarray technology, thereby providing a theoretical basis for further structure-activity study and minimal antigenic epitope screening. The disclosure has excellent application prospects in the aspects of development of V. cholerae synthetic glycoconjugate vaccines and new drugs, etc.

The invention relates to the technical field of molecular diagnosis and graphic processing, in particular to an electronic microarray chip dot matrix negative and positive judgment method based on an ISFET sensor. A chip hole site corresponds to an ISFET sensor, and after a hole bottom specific nucleic acid probe and a target sequence are hybridized and extended, the voltage of the sensor is changed. The method specifically comprises the following steps: data preprocessing: filtering median values of multi-frame electric signals of a hole site and taking a mean value; the dot matrix identification comprises the steps of threshold demarcation, Canny edge detection, circular Hough transform, optimal circle screening by Kdtree, array numbering and abnormal deletion processing to position a dot matrix; and calculating a result, extracting a signal difference value of the D1 stage and the D4 stage, evaluating numerical values of the positive quality control array and the to-be-detected sample array through a numerical value of the negative quality control array, and judging a final calculation result. According to the method, response changes are rapidly analyzed through image recognition, false detection and missing detection caused by numerical value differences in different arrays are reduced through multiple times of detection, and the detection accuracy is improved by selecting the most possible area through tickets.

The invention discloses a carbon-based microarray pressure sensor for pneumatic monitoring of an airfoil and a preparation method, belongs to the technical field of wind tunnel experimental equipment and advanced sensors, and aims to solve the problem of how to realize high-density, synchronous and accurate measurement of dynamic and static pressure of the airfoil surface on the premise of not damaging the airfoil structure. The sensor comprises a flexible substrate, a dynamic pressure sensitive element and a static pressure sensitive element, the flexible substrate is of a three-layer structure with gradient modulus; the dynamic pressure sensitive element is arranged on the flexible substrate and is used for sensing dynamic pressure; the static pressure sensitive element is arranged on the dynamic pressure sensitive element and is used for sensing static pressure; wherein the flexible substrate comprises an adhesion bottom layer, a middle buffer layer and a top supporting layer, the adhesion bottom layer is adhered to a wing surface, and bi-component microcapsules are dispersed in the adhesion bottom layer; the top supporting layer is provided with a micro-island bridge array structure; and the dynamic pressure sensitive element is fixedly supported on the micro-island bridge array.

The present invention discloses a polymer molding detection device based on piezoelectric-thermocouple array and optical integration, wherein the basic components include: a measurement channel, an outer edge layer, and an insulating sleeve arranged coaxially from the inside to the outside; the measurement channel is a microarray structure channel, in which the polymer melt flows axially; the temperature control component includes a heating coil and a cooling water channel, the heating coil is arranged on the outside of the outer edge layer, and the cooling water channel is arranged in the outer edge layer; the microarray component includes two thermocouple sensor arrays and two piezoelectric sensor arrays of equal thickness; the two thermocouple sensor arrays are arranged relatively on the surface of the inner wall of the measurement channel, the piezoelectric sensor array and the thermocouple sensor array are arranged in a stacked manner, and the positions of the two piezoelectric sensor arrays are relative; the optical axis of the optical-stress measurement component is perpendicular to the axis of the measurement channel, and the internal stress of the polymer melt is detected by polarized light and a CCD camera. The present invention can realize the detection of global polymer melt information in the micro-molding measurement channel.

Described herein are biosensor microarrays comprising detector polypeptide monolayers substantially free of contaminants. Also provided are methods for generation of such biosensor microarrays by capture of polypeptides by arrays comprising capture moieties and associated sensors.

The application provides a preparation method of a security label and the security label, and the preparation method comprises: sequentially forming a first security structure and a second security structure in a laminated arrangement on a surface of a substrate, forming the first security structure comprises forming a perovskite film on the surface of the substrate, the perovskite film can be irradiated by a first light beam to present a first preset pattern, and forming the second security structure comprises forming a structural color film on a side of the perovskite film away from the substrate, the structural color film can be irradiated by a second light beam to present a second preset pattern. Wherein, the structural color film is formed by irradiation light sequentially irradiating through a first mask plate and a second mask plate, a mask pattern of the first mask plate is the same as the second preset pattern, and the second mask plate is a first-pitch grating, so that the structural color film presents a microarray structure, based on the microarray structure, the structural color pattern and the fluorescent pattern can be selectively hidden and displayed, and then information encryption of the security structure is realized.

On an array surface (32) of a microlens array (31), lenses are arranged in a lattice shape. The lenses (30) have convex surfaces which are cylindrical through cross sections parallel to a lattice direction of the lenses (30) and orthogonal to the array surface (32). In each meridian on each cross section of the lenses (30) parallel to the lattice direction, the sag is corrected so that the inclination of the skirt becomes large. In other modes, the lenses have concave surfaces which are cylindrical through cross sections parallel to a lattice direction of the lenses and orthogonal to the array surface. In each meridian on each cross section of the lenses parallel to the lattice direction, the sag is corrected so that the inclination of the edge becomes large.

A removable cartridge to be used in a system for extracting and detecting nucleic acids from heterogeneous samples includes a plurality of reservoirs defining at least a first wash buffer reservoir for holding a first wash buffer and a microfluidic assembly configured to attach to the plurality of reservoirs. The microfluidic assembly includes at least one sample reservoir and a nucleic acid extraction matrix in fluid communication to an automated sample preparation (ASP) reservoir through a first flow channel defined by the microfluidic assembly. An assay chamber is in fluid communication with a third flow channel and with the waste reservoir through a fourth flow channel such that a labeled nucleic acid-containing sample flows through the assay chamber and then to the waste reservoir, wherein vibration-driven mixing agitates fluids while present in the assay chamber. Finally, a nucleic acid-detecting microarray module is positioned in the assay chamber.

The invention discloses a biological chip for detecting C-reactive protein as well as a preparation method and application of the biological chip. The biochip comprises a solid phase carrier and an immunodetection system, the solid phase carrier comprises a substrate and a glue layer, the immunodetection system comprises a microarray and a biological material, and the biological material is used for targeted recognition of a target object to be detected and is selected from an aptamer or an antibody. The lowest detection limit value of the biochip is 1.1 ng / mL, and the biochip can be used for rapidly and ultrasensitively detecting the C-reactive protein, is suitable for rapid detection of patients by basic medical institutions, and has great significance in rapid screening of patients in remote areas or outpatient service.

A supervised learning method and system for explicit spatial filtering of speech are disclosed. According to an embodiment, the supervised learning method for spatial filtering of speech, performed by a beamformer learning system, includes: receiving, as input into a neural network-based beamformer model, a multi-channel speech signal incident on a microarray in a reverberant environment and a beam condition representing the direction of interest (DOI); and outputting a desired signal corresponding to the beam condition from the multi-channel speech signal by using the neural network-based beamformer model, wherein the neural network-based beamformer model is trained to extract a speech signal with azimuth and elevation angles that are set for the beam condition, by using training data.

Dispensable formulations, microneedles, microarray patches (MAPs), and systems that can facilitate simple, substantially pain-free, and consistent delivery of a wide range of active pharmaceutical ingredients (APIs) and doses, as well as methods of manufacturing and using the same are described herein.