245 results about "Microarray" patented technology

The invention belongs to the technical field of biological detection, particularly relates to a gold nano pumpkin array, and relates to an SERS (Surface Enhanced Raman Scattering) sensor of a gastric precancerous lesion biomarker as well as a preparation method and application thereof. The preparation method of the gold nano pumpkin arrays (Au NPAs) comprises the steps that a PS single-layer template is obtained through a gas-liquid interface self-assembly technology, the PS single-layer template is sequentially subjected to plasma etching technology treatment, annealing treatment and ion sputtering treatment, and the gold nano pumpkin arrays (Au NPAs) are obtained. The SERS sensor and the SERS microarray chip constructed on the basis of the gold nano pumpkin array show excellent chemical stability, high molecular specificity and excellent reproducibility, ultra-trace detection of gastric cancer precancerous lesion biomarkers miR-196b and miR-221 is realized, and technical support is provided for early discovery, timely intervention and treatment of GC (Gas Chromatography).

The invention relates to a method for producing nano-arrays and micro-arrays. The method described herein provides a method of generating an array of spatially separated proteins. The method relies on covalently attaching each of a plurality of proteins to a structured nucleic acid particle (SNAP) and attaching the SNAP to a solid support.

The invention discloses an inflammatory bowel disease activity evaluation system based on multi-modal endoscopic feature fusion, and relates to the field of medical information processing. The system comprises a multi-modal data receiving module used for receiving first EUS microarray multi-modal endoscope dynamic sequence data; the image preprocessing module is used for carrying out noise reduction processing and determining a first white light effective observation image and a first ultrasonic effective observation image; the white light surface feature extraction module is used for extracting mucous membrane surface features by using a white light surface feature extraction model to determine mucous membrane features under a first white light endoscope; the ultrasonic transmural feature extraction module is used for performing structure identification and measurement by using an ultrasonic transmural feature extraction model to determine first ultrasonic lower mucous membrane features; and the multi-modal endoscope IBD activity evaluation module is used for determining the IBD activity based on the multi-modal endoscope characteristics by using the multi-modal dynamic endoscope IBD activity evaluation model. Compared with the prior art, the method has the advantages that the limitation of disease assessment based on single-mode and static pictures in the past is broken through, and the accuracy of IBD activity assessment results is improved.

The invention provides a sika deer antler heavy whole genome SNP site combination, a probe, a chip and application thereof, and relates to the technical field of molecular breeding. The sika deer antler weight whole genome SNP locus combination comprises 6302 SNP loci, the number of the loci is sufficient, the loci can fully cover a main genetic area affecting deer antler weight, and the accuracy, stability and repeatability of genetic evaluation are effectively improved; the Northeast sika deer capture efficiency evaluation is carried out on a Northeast sika deer sample based on a probe designed on 6302 SNP loci, the capture proportion is kept at 98.60%-99.80%, the overall capture efficiency is high, the stability is good, and the Northeast sika deer capture efficiency evaluation method has good application value.

The disclosure discloses a chemical synthesis method and an application of V. cholerae serotype O100 O-antigen oligosaccharides, belonging to the field of chemical technologies. The disclosure uses three monosaccharide building blocks and five carboxylic acid derivatives to synthesize five oligosaccharide fragments of V. cholerae serotype O100 O-antigen under the action of the solvent effect, temperature effect, neighboring group participation effect, etc., through orthogonal protection, selective assembly and amide coupling. The absolute configurations and immunological effects of 3,5-dihydroxyhexanoyl in the O-antigen trisaccharide are illustrated by the synthesized oligosaccharide fragments in combination with the NMR analysis and glycan microarray technology, thereby providing a theoretical basis for further structure-activity study and minimal antigenic epitope screening. The disclosure has excellent application prospects in the aspects of development of V. cholerae synthetic glycoconjugate vaccines and new drugs, etc.

The invention relates to the technical field of molecular diagnosis and graphic processing, in particular to an electronic microarray chip dot matrix negative and positive judgment method based on an ISFET sensor. A chip hole site corresponds to an ISFET sensor, and after a hole bottom specific nucleic acid probe and a target sequence are hybridized and extended, the voltage of the sensor is changed. The method specifically comprises the following steps: data preprocessing: filtering median values of multi-frame electric signals of a hole site and taking a mean value; the dot matrix identification comprises the steps of threshold demarcation, Canny edge detection, circular Hough transform, optimal circle screening by Kdtree, array numbering and abnormal deletion processing to position a dot matrix; and calculating a result, extracting a signal difference value of the D1 stage and the D4 stage, evaluating numerical values of the positive quality control array and the to-be-detected sample array through a numerical value of the negative quality control array, and judging a final calculation result. According to the method, response changes are rapidly analyzed through image recognition, false detection and missing detection caused by numerical value differences in different arrays are reduced through multiple times of detection, and the detection accuracy is improved by selecting the most possible area through tickets.

The invention discloses a carbon-based microarray pressure sensor for pneumatic monitoring of an airfoil and a preparation method, belongs to the technical field of wind tunnel experimental equipment and advanced sensors, and aims to solve the problem of how to realize high-density, synchronous and accurate measurement of dynamic and static pressure of the airfoil surface on the premise of not damaging the airfoil structure. The sensor comprises a flexible substrate, a dynamic pressure sensitive element and a static pressure sensitive element, the flexible substrate is of a three-layer structure with gradient modulus; the dynamic pressure sensitive element is arranged on the flexible substrate and is used for sensing dynamic pressure; the static pressure sensitive element is arranged on the dynamic pressure sensitive element and is used for sensing static pressure; wherein the flexible substrate comprises an adhesion bottom layer, a middle buffer layer and a top supporting layer, the adhesion bottom layer is adhered to a wing surface, and bi-component microcapsules are dispersed in the adhesion bottom layer; the top supporting layer is provided with a micro-island bridge array structure; and the dynamic pressure sensitive element is fixedly supported on the micro-island bridge array.

Described herein are biosensor microarrays comprising detector polypeptide monolayers substantially free of contaminants. Also provided are methods for generation of such biosensor microarrays by capture of polypeptides by arrays comprising capture moieties and associated sensors.

The application provides a preparation method of a security label and the security label, and the preparation method comprises: sequentially forming a first security structure and a second security structure in a laminated arrangement on a surface of a substrate, forming the first security structure comprises forming a perovskite film on the surface of the substrate, the perovskite film can be irradiated by a first light beam to present a first preset pattern, and forming the second security structure comprises forming a structural color film on a side of the perovskite film away from the substrate, the structural color film can be irradiated by a second light beam to present a second preset pattern. Wherein, the structural color film is formed by irradiation light sequentially irradiating through a first mask plate and a second mask plate, a mask pattern of the first mask plate is the same as the second preset pattern, and the second mask plate is a first-pitch grating, so that the structural color film presents a microarray structure, based on the microarray structure, the structural color pattern and the fluorescent pattern can be selectively hidden and displayed, and then information encryption of the security structure is realized.

On an array surface (32) of a microlens array (31), lenses are arranged in a lattice shape. The lenses (30) have convex surfaces which are cylindrical through cross sections parallel to a lattice direction of the lenses (30) and orthogonal to the array surface (32). In each meridian on each cross section of the lenses (30) parallel to the lattice direction, the sag is corrected so that the inclination of the skirt becomes large. In other modes, the lenses have concave surfaces which are cylindrical through cross sections parallel to a lattice direction of the lenses and orthogonal to the array surface. In each meridian on each cross section of the lenses parallel to the lattice direction, the sag is corrected so that the inclination of the edge becomes large.

A removable cartridge to be used in a system for extracting and detecting nucleic acids from heterogeneous samples includes a plurality of reservoirs defining at least a first wash buffer reservoir for holding a first wash buffer and a microfluidic assembly configured to attach to the plurality of reservoirs. The microfluidic assembly includes at least one sample reservoir and a nucleic acid extraction matrix in fluid communication to an automated sample preparation (ASP) reservoir through a first flow channel defined by the microfluidic assembly. An assay chamber is in fluid communication with a third flow channel and with the waste reservoir through a fourth flow channel such that a labeled nucleic acid-containing sample flows through the assay chamber and then to the waste reservoir, wherein vibration-driven mixing agitates fluids while present in the assay chamber. Finally, a nucleic acid-detecting microarray module is positioned in the assay chamber.

Dispensable formulations, microneedles, microarray patches (MAPs), and systems that can facilitate simple, substantially pain-free, and consistent delivery of a wide range of active pharmaceutical ingredients (APIs) and doses, as well as methods of manufacturing and using the same are described herein.

This invention belongs to the field of biomedical engineering technology, providing a method for preparing modular bio-ink for cell spheres and its application in the field of myocardial repair therapy. First, a method for preparing stem cell spheres based on microarrays is described. By introducing a protein / polyphenol composite coating onto the cell spheres, a multifunctional armor is created, providing immune protection and immunomodulatory therapy against the host's immune system to reverse the hostile microenvironment and promote the healing process of infarcted myocardial tissue. Second, using microfluidic droplet control technology, gel microspheres loaded with bioactive substances are prepared from biocompatible biomaterials. These microspheres are then densely packed and coated to form cell sphere particle gels. These gels can be used for disease treatment via direct injection or bio-3D printing, showing promising application prospects.

A microarray patch (1) is disclosed comprising a substrate (2) and an array of microneedles (3) arranged on the substrate (2). The substrate (2) is configured to indicate detection of moisture. The substrate (2) may comprise a dye that changes color upon contact with moisture. The color change of the substrate is configured to coincide with delivery of a substance through or from the microneedle or capture of the substance into the microneedle. The dye may be configured to produce a gradual color change as a function of the degree of wetting. The substrate (2) may be insoluble in water and hydrophobic. A method of manufacturing a microarray patch (1) is also disclosed.

This invention discloses a droplet microarray chip based on molecular logic operations and its construction method, belonging to the field of biochemical analysis and detection technology. It includes a substrate layer, a functional layer on the substrate layer, and molecular logic circuitry. The functional layer is a superhydrophilic-superhydrophobic patterned porous polymer film prepared in situ on the substrate using digital light processing technology, followed by PFOC acylation. The superhydrophilic-superhydrophobic patterned porous polymer film contains arrayed superhydrophilic microreaction units, separated by superhydrophobic polymer regions. Each superhydrophilic microreaction unit encapsulates an independent microdroplet, and the molecular logic circuitry is encapsulated within each independent microdroplet. This invention, through the combination of droplet microarray chip and molecular logic circuitry, achieves systematic improvements in multi-enzyme synergistic detection capabilities, logic computation complexity, spatial isolation control, high-throughput analysis, and adaptation to biological environments.

The invention discloses a sub-wavelength metal wire grating microarray polarization parameter reconstruction method and device, a medium and equipment. The method comprises the following steps: obtaining detection light intensity of a target in a fixed angle direction and detection light intensity change; obtaining polarization detection light intensity based on the sum value of the detection light intensity in each fixed direction and the detection light intensity change; obtaining a first target polarization detection vector matrix according to the Stokes parameter matrix; replacing the detection light intensity in the first target polarization detection vector matrix with the polarization detection light intensity to obtain a second target polarization detection vector matrix; and inputting the second target polarization detection vector matrix and a polarization transmittance parameter, an extinction ratio parameter and a uniformity parameter of the sub-wavelength metal wire grating microarray into a pre-constructed multi-parameter joint constraint polarization parameter reconstruction network, and outputting polarization energy, polarization degree and polarization angle information after target reconstruction. According to the invention, high-precision reconstruction of polarization energy parameters, polarization degree parameters and polarization angle parameters can be provided.

The invention relates to a method for microarray transformation, wherein, by using a cavity chip with transformation matrix, a template array can be copied onto a planar support, and the information or spatial arrangement is changed in the process, so that a transformed second array forms. The invention further relates to a device for carrying out such a method.

The present disclosure relates to the field of molecular biology and more specifically to microarrays and methods. In particular, the present disclosure relates to a method for amplifying a nucleic acid on a substrate comprising at least one well, the method includes: depositing a plurality of first capture primer pairs and a plurality of second capture primer pairs in a layer at least partially covering the inner well surface, wherein the primer density of first capture primer pairs is higher than the primer density of second primer pairs; contacting a sample comprising a plurality of target polynucleotides with the substrate under conditions sufficient for a single target polynucleotide per well to hybridize with a capture primer of the second capture primer pair; and performing a kinetic exclusion assay (KEA) to produce a monoclonal population of amplicons from the single target polynucleotide.

The application discloses a kind of based on flow rate control's giant liposome preparation, collection device in microfluidic field, comprising: upper electrode;Lower electrode, surface is equipped with microarray structure;Fluid channel layer is arranged between upper electrode and lower electrode, and is communicated with microarray structure, fluid channel layer is used to pass into fluid to complete collection to liposome;And a kind of preparation, collection method;The beneficial effects of the application are: by setting upper electrode, lower electrode and the fluid channel layer between the two, provide environment for the preparation and collection of liposome, the liposome prepared and collected by the device and method is more complete, the quality of collected liposome is higher, more conducive to subsequent research.

1. Name of the Design Product: Spectrophotometer (Nano-8000). 2. Use of the Design Product: Detect nucleic acids, proteins, cell solutions, microarray samples, and perform conventional full-wavelength scanning, etc. 3. Design Key Points of the Design Product: Lies in the shape. 4. Picture or Photo that Best Illustrates the Design Key Points: Front View.

The invention provides a corneal limbus stem cell in-situ repair microneedle array patch and a preparation method and application thereof.The corneal limbus stem cell in-situ repair microneedle array patch comprises a biocompatible backing layer and a microarray layer arranged on the biocompatible backing layer, microneedles of the microarray layer are of a conical structure, the height is 100-300 micrometers, the vertex angle is smaller than 30 degrees, and the thickness of the microneedles of the microarray layer is 10-30 micrometers. And a nano-scale porous structure for loading cornea stem cell suspension is arranged in the cornea stem cell support. Through precise geometric design, biomimetic material selection and spatial arrangement optimization, in-situ delivery, microenvironment activation and tissue fusion in a corneal limbus area are realized under the condition of not depending on in-vitro amplification and surgical suture, and the method becomes an important direction for promoting LSCD minimally invasive treatment strategy innovation.

There is provided a fluorescence reading device which acquires fluorescence from a microarray, including: a receiving optical system which repeatedly reflects fluorescence emitted from the microarray off a plurality of reflectors to form an image on an image-forming plane; and an optical sensor located on the image-forming plane. The fluorescence reading device may be a telecentric optical system in which an image-capturing plane of the microarray and an image-forming plane are the same in size. The plurality of reflectors may include a convex mirror and a concave mirror. The plurality of reflectors may include only one convex mirror and one concave mirror.

The invention discloses a hybrid control method suitable for a long-stroke rapid cutter servo system, and the method comprises the steps: obtaining a controlled object model according to the sweep frequency of an open-loop system and system identification, and constructing a closed-loop hybrid control algorithm model based on proportional integral differential control, sliding mode control and speed / acceleration feedforward control; by continuously calculating system output and control errors, parameter adjustment of a control algorithm is completed; the hybrid control algorithm model is written into PMAC hardware, step and sine response testing and control parameter optimization adjustment are completed, and high-precision machining of the long-stroke rapid tool servo system is achieved. According to the hybrid control method, the response speed and the control precision of the system can be remarkably improved, nonlinear disturbance in a complex machining task can be adapted, and a high-stability and low-inertia-force solution is provided for ultra-precision machining of a surface microarray, an optical free-form surface and the like.

The present invention relates to a composition for alleviating skin irritation caused by vitamin C, and an information providing method for selecting a custom-made substance for alleviating irritation caused by vitamin C, comprising a step of confirming a base of a polymorphic site of a gene polymorphic marker which is significant in relation to the degree of irritation caused by vitamin C. The invention also relates to a composition for alleviating skin irritation caused by vitamin C and an information providing method for selecting the custom-made substance for alleviating irritation caused by vitamin C. Furthermore, the present invention relates to: a composition for diagnosing whether or not a skin type in which skin irritation caused by vitamin C is likely to occur, the composition comprising a probe capable of detecting a gene polymorphism marker having significance in association with skin irritation caused by vitamin C or a preparation capable of amplifying the gene polymorphism marker; a kit or a microarray comprising the composition; and a method for providing information on the type of skin, which is susceptible to skin irritation caused by vitamin C, using the gene polymorphism marker or the combination of markers.

The present invention relates to a plasmonic microarray-based multiple detection kit for genetic mutations, and a multiple detection method for genetic mutations, using same, and, specifically, to a plasmonic microarray-based multiple detection kit for genetic mutations, and a multiple detection method for genetic mutations, using same, the kit comprising: a nucleic acid amplification composition for a target gene; a multi-analysis substrate on which a capture probe for capturing both a wild-type gene and a mutation-type gene of the target gene as nucleic acid amplification products is fixed in an array form on a surface fluorescence amplification substrate; and a fluorescent probe that binds to both the wild-type gene and the mutation-type gene captured by the capture probe, wherein the nucleic acid amplification composition includes: a nucleic acid amplification primer set that can amplify both the wild-type gene and the mutation-type gene of the target gene; and a probe having a quencher that binds to the wild-type gene.

A microarray manufacturing apparatus according to an embodiment of the present invention comprises a microarray tip manufacturing apparatus and a microarray base manufacturing apparatus. The tip manufacturing apparatus includes: a dispensing unit that dispenses a tip composition including an active ingredient into an engraved portion of a supplied mold; and a first filling unit that simultaneously performs filling, in which even fine structures in the engraved portion of the mold are filled with the tip composition dispensed into the engraved portion of the mold, and drying of the filled composition. The base manufacturing apparatus includes: a dispensing unit that receives the mold, in which the tip composition has been filled and dried, and dispenses a base composition onto the tip composition filled and dried inside the engraved portion of the mold; and a second filling unit that simultaneously performs filling and drying of the base composition dispensed into the engraved portion of the mold.

The invention discloses a chemical sensing system based on graphene. The system includes a sensor module including a substrate, a plurality of pairs of electrodes disposed over the substrate, and a sensing layer disposed over each pair of electrodes on the substrate to form an array of sensing surfaces. The sensing layer is an integrated surface functionalization mask having an open slot for each sensing surface. Each open slot includes a different polymeric material. The surface functionalization mask enables simultaneous deposition of various polymeric materials to the sensing surface using microarray printing. The sensor module is configured to create sensor data when a chemical compound is presented on the sensing surface and a constant bias is applied on the electrodes, the sensor data having a cross-reaction response characteristic resulting from a temporary change in resistance of the graphene over time. The substrate further includes silicon (Si), thermally grown silicon dioxide (SiO2), and graphene. The electrode comprises a titanium / gold (Ti / Au) interdigital electrode.

This invention discloses a system for assessing the activity of inflammatory bowel disease (IBD) based on multimodal endoscopic feature fusion, relating to the field of medical information processing. The system includes: a multimodal data receiving module for receiving dynamic sequence data from a first EUS microarray multimodal endoscopy; an image preprocessing module for noise reduction and determining first effective white light observation images and first effective ultrasound observation images; a white light surface feature extraction module for extracting mucosal surface features using a white light surface feature extraction model to determine the first white light endoscopic mucosal features; an ultrasound transmural feature extraction module for structural identification and measurement using an ultrasound transmural feature extraction model to determine the first ultrasound-guided mucosal features; and a multimodal endoscopic IBD activity assessment module for determining IBD activity based on multimodal endoscopic features using a multimodal dynamic endoscopic IBD activity assessment model. Compared to existing technologies, this invention overcomes the limitations of previous disease assessments based on single-modality, static images, improving the accuracy of IBD activity assessment results.

The invention discloses a functionalized microarray pore plate, a preparation method thereof and application of the functionalized microarray pore plate in membrane protein ligand screening, and belongs to the technical field of biological medicine analysis. Amino and vinyl sulfuryl are covalently modified on the surface of the microarray pore plate to introduce an active site which can be specifically covalently bound with a membrane protein or a tag group, so that directional fixation of a target membrane protein on the surface of the microarray pore plate is realized, and the functionalized microarray pore plate is obtained. A functional microarray pore plate and a specific fluorescent probe are combined to screen a membrane protein ligand, the specific fluorescent probe is combined with a receptor active site of a fixed protein, and a detectable fluorescence signal change is generated by utilizing a competitive replacement mechanism of the probe and a candidate compound at the active site; the method is used for in-vitro screening and discovery of potential membrane protein ligands.

The invention provides a tumor in-situ pathology detection system based on an endoscopic multi-mode probe. The tumor in-situ pathology detection system comprises a main controller, an SC laser, a DUV laser, a comb filter, a polarizer, a microarray lens, a multiplexing beam combiner, a conjugate coupling module, an endoscopic optical fiber bundle, a FAST optical fiber bundle, an SC spectrograph and a DUV spectrograph. Aiming at G14-level bronchial navigation surgical robot in-situ pathology detection, the invention provides a detection system based on an endoscopic multi-mode optical probe, FAST hyperspectral image spectrum integrated detection of SC, DUVRS, DUVLIF and mIHC can be realized, a multi-mode hyperspectral fusion image reflecting spatial distribution of histochemical components of a diagnosis point and corresponding spectral feature data are output, and the detection system is used for detecting the in-situ pathology of the G14-level bronchial navigation surgical robot. And real-time, accurate and multi-mode data or information is provided for diagnosis of doctors.