303 results about "Microarray" patented technology

The invention relates to the technical field of medical treatment, and particularly discloses a minimally invasive implantable brain-computer interface intracranial multi-mode signal monitoring system, which comprises a flexible probe, a data acquisition module, a data return module and a data analysis module, the flexible probe is embedded into the high-precision microarray electrode and the sensor to collect neural signals in real time; the data acquisition module is used for synchronously acquiring electroencephalogram signals and intracranial pressure of specific parts of the brain; the data return module manages the collected data; and the data analysis module analyzes the electroencephalogram signal and the intracranial pressure data. According to the minimally invasive implantable brain-computer interface intracranial multi-mode signal monitoring system provided by the invention, lumbar puncture enters a cerebrospinal fluid space and is sent to a target spot, so that the infection risk can be effectively reduced, the limitation on the action of a patient is reduced, and the system has the characteristics of real-time performance, directness and accuracy; the method has very important significance and value for evaluating brain injury and brain functional lesion of a patient, prognosis judgment and the like.

The invention discloses a temperature correction method of a chloride ion sensor, which comprises the following steps of: S1, arranging a micro array type chloride ion sensor of a pre-embedded concrete protective layer in an array mode, and determining an acquisition scheme; s2, carrying out anomaly detection on a MnO2 reference electrode in the micro array chloride ion sensor; s3, correcting an acquired potential value of the micro array type chloride ion sensor; s4, according to the Nernst potential-concentration logarithm linear relation, determining various coefficients of the standard equation under the control temperature in the concrete; and S5, determining a temperature compensation coefficient according to the Nernst potential-concentration logarithm linear relationship. And S6, calculating the concentration distribution condition of chloride ions at different depths in the concrete by using the sensor potential after temperature compensation. According to the invention, the influence of temperature on the measurement result of the chloride ion sensor can be effectively compensated, the potential abnormal interference in the measurement process of the sensor is effectively overcome, and the precision and reliability of the sensor are improved.

Microarray applicator (10000) including a microarray carrier (8000), which is retained within a housing (2000) by virtue of microarray carrier (8100) being engaged withholder (2400). Microarray patch (8200) and flexible backing (8220) are positioned such that the central portion (8210) has microneedles (8211) protrude in the direction opposite from the bottom (7200) of plunger (7000). Plunger (7000) is in a first position, having first notch (7310) engaged with ridge (2320). After activation, wherein plunger (7000) is in a second position such that second notch (7320) is engaged with ridge (2320). Here, bottom (7200) of plunger (7000) extends below first major surface (2100) of housing (2000) and has ejected microarray patch (8200).

The invention belongs to the technical field of biological detection, particularly relates to a gold nano pumpkin array, and relates to an SERS (Surface Enhanced Raman Scattering) sensor of a gastric precancerous lesion biomarker as well as a preparation method and application thereof. The preparation method of the gold nano pumpkin arrays (Au NPAs) comprises the steps that a PS single-layer template is obtained through a gas-liquid interface self-assembly technology, the PS single-layer template is sequentially subjected to plasma etching technology treatment, annealing treatment and ion sputtering treatment, and the gold nano pumpkin arrays (Au NPAs) are obtained. The SERS sensor and the SERS microarray chip constructed on the basis of the gold nano pumpkin array show excellent chemical stability, high molecular specificity and excellent reproducibility, ultra-trace detection of gastric cancer precancerous lesion biomarkers miR-196b and miR-221 is realized, and technical support is provided for early discovery, timely intervention and treatment of GC (Gas Chromatography).

The invention discloses sand grains with a water storage structure and a preparation method and application thereof. The method comprises the steps that S1, sand grains with the grain size ranging from 0.1 mm to 1 mm are selected, the grain size distribution meets the condition that D50 is equal to 0.3 mm, clay impurities are screened out, and the sand grains are placed on a sand grain base plate (1) after being cleaned and dried; s2, nano silicate powder is uniformly sprayed on the surfaces of the sand grains through an electrostatic spraying technology, so that a silicate glass layer (5) is formed on the surfaces of the sand grains; s3, a microarray lens (3) is used for dividing a laser source into multiple laser beams, each laser beam irradiates the silicate glass layer (5) on each sand grain in a one-to-one mode, silicate powder and the surface layers of the sand grains are co-melted, and then an amorphous net-shaped structure is formed; s4, argon is used for spraying the amorphous net-shaped structure so that the amorphous net-shaped structure can be rapidly cooled, and then a porous heterojunction structure is formed on the surface of the sand grains; and S5, scanning the silicate glass layer (5) by using the laser again, so that the part of the silicate glass layer (5) is molten again, silicate is decomposed to generate gas, and cooling and curing are performed after bubbles are formed in the molten glass. According to the invention, the sand grains have a certain water storage characteristic.

The invention relates to a method for producing nano-arrays and micro-arrays. The method described herein provides a method of generating an array of spatially separated proteins. The method relies on covalently attaching each of a plurality of proteins to a structured nucleic acid particle (SNAP) and attaching the SNAP to a solid support.

The invention discloses an inflammatory bowel disease activity evaluation system based on multi-modal endoscopic feature fusion, and relates to the field of medical information processing. The system comprises a multi-modal data receiving module used for receiving first EUS microarray multi-modal endoscope dynamic sequence data; the image preprocessing module is used for carrying out noise reduction processing and determining a first white light effective observation image and a first ultrasonic effective observation image; the white light surface feature extraction module is used for extracting mucous membrane surface features by using a white light surface feature extraction model to determine mucous membrane features under a first white light endoscope; the ultrasonic transmural feature extraction module is used for performing structure identification and measurement by using an ultrasonic transmural feature extraction model to determine first ultrasonic lower mucous membrane features; and the multi-modal endoscope IBD activity evaluation module is used for determining the IBD activity based on the multi-modal endoscope characteristics by using the multi-modal dynamic endoscope IBD activity evaluation model. Compared with the prior art, the method has the advantages that the limitation of disease assessment based on single-mode and static pictures in the past is broken through, and the accuracy of IBD activity assessment results is improved.

Disclosed in the present invention are a solid electrolyte membrane, and a preparation method therefor and the use thereof. The solid electrolyte membrane comprises a microarray support membrane and a solid electrolyte material loaded on the microarray support membrane, wherein the microarray support membrane is provided with an array-shaped micropore structure, the duty ratio of the micropore structure being 50%-99%, and the diameter of micropores in the micropore structure being 1-500 μm. The solid electrolyte membrane of the present invention has the effect of homogenizing current density, and is characterized by being ultrathin, highly flexible, capable of homogenizing current density, etc.

The invention belongs to the technical field of medicine, and particularly relates to application of a small molecule compound R406 in preparation of a product for preventing and treating Parkinson's disease, screening based on small molecules and microarrays is carried out, the compound R406 can reduce the alpha-syn protein level in an autophagy-dependent mode, and in addition, the compound R406 can be used for preparing a product for preventing and treating Parkinson's disease. The R406 also reduces the level of alpha-syn phosphorylated serine 129 in a fiber-induced cell model pre-formed by alpha-syn, and prevents neuronal degeneration. In addition, experiments confirm that the R406 can relieve PD-related disease phenotypes in a human alpha-syn preformed fiber-induced cell model and a PD patient-derived organ-like model, and a new thought is provided for treatment of the Parkinson's disease.

The invention provides a low-light-level enhancement camera imaging enhancement method and system, and the method comprises the steps: obtaining a target video shot by a microarray fly's-eye lens camera, carrying out the frame-by-frame splitting processing of the target video, so as to obtain a plurality of target video frames, and carrying out the preprocessing of the corresponding target video frames, so as to obtain a plurality of target images; establishing an initial filter, performing updating processing on the initial filter to obtain an updated filter, and performing adaptive filtering processing on the target image based on the updated filter to obtain a filtered image; identifying a noise level of the filtered image, and determining a low-light image frame based on the noise level; gaussian filtering processing and detail enhancement are carried out on the low-light image frame; according to the method, the corresponding low-light-level image is determined according to the noise level of the image, enhancement processing is carried out on the low-light-level image, the calculation complexity is remarkably reduced, and super-resolution enhancement of the low-light-level image is achieved.

The invention provides a method for machining a straight micro through hole in glass through ultrafast laser and a machining system. The method comprises the following steps that three glass workpieces are bonded into a sandwich type workpiece through an optical cement technology; the Bessel beam is focused on the sandwich type workpiece by adjusting the length of the Bessel beam; ultrafast laser irradiates the sandwich type workpiece, so that the material structure of the inner area of the glass is converted into a silicon-oxygen three-membered ring or a silicon-oxygen four-membered ring from a silicon-oxygen six-membered ring, and the sandwich type workpiece with a vertically-through modified microarray area inside is obtained after processing; the sandwich type workpiece after laser processing is separated through the optical cement removing technology, and only the target workpiece in the middle is reserved; putting a target workpiece into an etching solution, introducing ultrasonic oscillation for etching, and corroding a silicon-oxygen three-membered ring or a silicon-oxygen four-membered ring in an internal modified microarray region by an etching agent to form a micro through hole; and cleaning the etched target workpiece. The appearance of a horn mouth can be avoided.

The invention discloses a microorganism-intestine-joint axis multi-organ chip and a preparation method thereof, and belongs to the field of biomedical engineering.The preparation method comprises the following specific steps that firstly, an upper-layer chip male mold, a middle-layer chip male mold and a lower-layer chip male mold of the multi-organ chip are prepared through the high-precision 3D printing technology; iI, processing the silicon wafer with the cylindrical microarray through photoetching to prepare a porous membrane; iII, preparing an upper-layer chip, a middle-layer chip and a lower-layer chip; iV, assembling each group of prepared chips; according to the invention, human cells or organoids can be used for experiments, the experiment result has good correlation with human beings, and there is no species difference between animal experiments and human beings; the experiment process can be obviously accelerated, and the experiment time is shortened; according to the method, the experimental cost can be greatly saved, and the dispute of experimental ethics does not exist, so that the method can be expanded to large-scale standardized processing production so as to carry out high-throughput and large-scale experimental research.

The invention provides a sika deer antler heavy whole genome SNP site combination, a probe, a chip and application thereof, and relates to the technical field of molecular breeding. The sika deer antler weight whole genome SNP locus combination comprises 6302 SNP loci, the number of the loci is sufficient, the loci can fully cover a main genetic area affecting deer antler weight, and the accuracy, stability and repeatability of genetic evaluation are effectively improved; the Northeast sika deer capture efficiency evaluation is carried out on a Northeast sika deer sample based on a probe designed on 6302 SNP loci, the capture proportion is kept at 98.60%-99.80%, the overall capture efficiency is high, the stability is good, and the Northeast sika deer capture efficiency evaluation method has good application value.

The disclosure discloses a chemical synthesis method and an application of V. cholerae serotype O100 O-antigen oligosaccharides, belonging to the field of chemical technologies. The disclosure uses three monosaccharide building blocks and five carboxylic acid derivatives to synthesize five oligosaccharide fragments of V. cholerae serotype O100 O-antigen under the action of the solvent effect, temperature effect, neighboring group participation effect, etc., through orthogonal protection, selective assembly and amide coupling. The absolute configurations and immunological effects of 3,5-dihydroxyhexanoyl in the O-antigen trisaccharide are illustrated by the synthesized oligosaccharide fragments in combination with the NMR analysis and glycan microarray technology, thereby providing a theoretical basis for further structure-activity study and minimal antigenic epitope screening. The disclosure has excellent application prospects in the aspects of development of V. cholerae synthetic glycoconjugate vaccines and new drugs, etc.

The invention relates to the technical field of molecular diagnosis and graphic processing, in particular to an electronic microarray chip dot matrix negative and positive judgment method based on an ISFET sensor. A chip hole site corresponds to an ISFET sensor, and after a hole bottom specific nucleic acid probe and a target sequence are hybridized and extended, the voltage of the sensor is changed. The method specifically comprises the following steps: data preprocessing: filtering median values of multi-frame electric signals of a hole site and taking a mean value; the dot matrix identification comprises the steps of threshold demarcation, Canny edge detection, circular Hough transform, optimal circle screening by Kdtree, array numbering and abnormal deletion processing to position a dot matrix; and calculating a result, extracting a signal difference value of the D1 stage and the D4 stage, evaluating numerical values of the positive quality control array and the to-be-detected sample array through a numerical value of the negative quality control array, and judging a final calculation result. According to the method, response changes are rapidly analyzed through image recognition, false detection and missing detection caused by numerical value differences in different arrays are reduced through multiple times of detection, and the detection accuracy is improved by selecting the most possible area through tickets.

The invention discloses a carbon-based microarray pressure sensor for pneumatic monitoring of an airfoil and a preparation method, belongs to the technical field of wind tunnel experimental equipment and advanced sensors, and aims to solve the problem of how to realize high-density, synchronous and accurate measurement of dynamic and static pressure of the airfoil surface on the premise of not damaging the airfoil structure. The sensor comprises a flexible substrate, a dynamic pressure sensitive element and a static pressure sensitive element, the flexible substrate is of a three-layer structure with gradient modulus; the dynamic pressure sensitive element is arranged on the flexible substrate and is used for sensing dynamic pressure; the static pressure sensitive element is arranged on the dynamic pressure sensitive element and is used for sensing static pressure; wherein the flexible substrate comprises an adhesion bottom layer, a middle buffer layer and a top supporting layer, the adhesion bottom layer is adhered to a wing surface, and bi-component microcapsules are dispersed in the adhesion bottom layer; the top supporting layer is provided with a micro-island bridge array structure; and the dynamic pressure sensitive element is fixedly supported on the micro-island bridge array.

The present invention discloses a polymer molding detection device based on piezoelectric-thermocouple array and optical integration, wherein the basic components include: a measurement channel, an outer edge layer, and an insulating sleeve arranged coaxially from the inside to the outside; the measurement channel is a microarray structure channel, in which the polymer melt flows axially; the temperature control component includes a heating coil and a cooling water channel, the heating coil is arranged on the outside of the outer edge layer, and the cooling water channel is arranged in the outer edge layer; the microarray component includes two thermocouple sensor arrays and two piezoelectric sensor arrays of equal thickness; the two thermocouple sensor arrays are arranged relatively on the surface of the inner wall of the measurement channel, the piezoelectric sensor array and the thermocouple sensor array are arranged in a stacked manner, and the positions of the two piezoelectric sensor arrays are relative; the optical axis of the optical-stress measurement component is perpendicular to the axis of the measurement channel, and the internal stress of the polymer melt is detected by polarized light and a CCD camera. The present invention can realize the detection of global polymer melt information in the micro-molding measurement channel.

Described herein are biosensor microarrays comprising detector polypeptide monolayers substantially free of contaminants. Also provided are methods for generation of such biosensor microarrays by capture of polypeptides by arrays comprising capture moieties and associated sensors.

The application provides a preparation method of a security label and the security label, and the preparation method comprises: sequentially forming a first security structure and a second security structure in a laminated arrangement on a surface of a substrate, forming the first security structure comprises forming a perovskite film on the surface of the substrate, the perovskite film can be irradiated by a first light beam to present a first preset pattern, and forming the second security structure comprises forming a structural color film on a side of the perovskite film away from the substrate, the structural color film can be irradiated by a second light beam to present a second preset pattern. Wherein, the structural color film is formed by irradiation light sequentially irradiating through a first mask plate and a second mask plate, a mask pattern of the first mask plate is the same as the second preset pattern, and the second mask plate is a first-pitch grating, so that the structural color film presents a microarray structure, based on the microarray structure, the structural color pattern and the fluorescent pattern can be selectively hidden and displayed, and then information encryption of the security structure is realized.

On an array surface (32) of a microlens array (31), lenses are arranged in a lattice shape. The lenses (30) have convex surfaces which are cylindrical through cross sections parallel to a lattice direction of the lenses (30) and orthogonal to the array surface (32). In each meridian on each cross section of the lenses (30) parallel to the lattice direction, the sag is corrected so that the inclination of the skirt becomes large. In other modes, the lenses have concave surfaces which are cylindrical through cross sections parallel to a lattice direction of the lenses and orthogonal to the array surface. In each meridian on each cross section of the lenses parallel to the lattice direction, the sag is corrected so that the inclination of the edge becomes large.

A removable cartridge to be used in a system for extracting and detecting nucleic acids from heterogeneous samples includes a plurality of reservoirs defining at least a first wash buffer reservoir for holding a first wash buffer and a microfluidic assembly configured to attach to the plurality of reservoirs. The microfluidic assembly includes at least one sample reservoir and a nucleic acid extraction matrix in fluid communication to an automated sample preparation (ASP) reservoir through a first flow channel defined by the microfluidic assembly. An assay chamber is in fluid communication with a third flow channel and with the waste reservoir through a fourth flow channel such that a labeled nucleic acid-containing sample flows through the assay chamber and then to the waste reservoir, wherein vibration-driven mixing agitates fluids while present in the assay chamber. Finally, a nucleic acid-detecting microarray module is positioned in the assay chamber.

The invention discloses a biological chip for detecting C-reactive protein as well as a preparation method and application of the biological chip. The biochip comprises a solid phase carrier and an immunodetection system, the solid phase carrier comprises a substrate and a glue layer, the immunodetection system comprises a microarray and a biological material, and the biological material is used for targeted recognition of a target object to be detected and is selected from an aptamer or an antibody. The lowest detection limit value of the biochip is 1.1 ng / mL, and the biochip can be used for rapidly and ultrasensitively detecting the C-reactive protein, is suitable for rapid detection of patients by basic medical institutions, and has great significance in rapid screening of patients in remote areas or outpatient service.

A supervised learning method and system for explicit spatial filtering of speech are disclosed. According to an embodiment, the supervised learning method for spatial filtering of speech, performed by a beamformer learning system, includes: receiving, as input into a neural network-based beamformer model, a multi-channel speech signal incident on a microarray in a reverberant environment and a beam condition representing the direction of interest (DOI); and outputting a desired signal corresponding to the beam condition from the multi-channel speech signal by using the neural network-based beamformer model, wherein the neural network-based beamformer model is trained to extract a speech signal with azimuth and elevation angles that are set for the beam condition, by using training data.

Dispensable formulations, microneedles, microarray patches (MAPs), and systems that can facilitate simple, substantially pain-free, and consistent delivery of a wide range of active pharmaceutical ingredients (APIs) and doses, as well as methods of manufacturing and using the same are described herein.

This invention belongs to the field of biomedical engineering technology, providing a method for preparing modular bio-ink for cell spheres and its application in the field of myocardial repair therapy. First, a method for preparing stem cell spheres based on microarrays is described. By introducing a protein / polyphenol composite coating onto the cell spheres, a multifunctional armor is created, providing immune protection and immunomodulatory therapy against the host's immune system to reverse the hostile microenvironment and promote the healing process of infarcted myocardial tissue. Second, using microfluidic droplet control technology, gel microspheres loaded with bioactive substances are prepared from biocompatible biomaterials. These microspheres are then densely packed and coated to form cell sphere particle gels. These gels can be used for disease treatment via direct injection or bio-3D printing, showing promising application prospects.

A microarray patch (1) is disclosed comprising a substrate (2) and an array of microneedles (3) arranged on the substrate (2). The substrate (2) is configured to indicate detection of moisture. The substrate (2) may comprise a dye that changes color upon contact with moisture. The color change of the substrate is configured to coincide with delivery of a substance through or from the microneedle or capture of the substance into the microneedle. The dye may be configured to produce a gradual color change as a function of the degree of wetting. The substrate (2) may be insoluble in water and hydrophobic. A method of manufacturing a microarray patch (1) is also disclosed.

This invention discloses a droplet microarray chip based on molecular logic operations and its construction method, belonging to the field of biochemical analysis and detection technology. It includes a substrate layer, a functional layer on the substrate layer, and molecular logic circuitry. The functional layer is a superhydrophilic-superhydrophobic patterned porous polymer film prepared in situ on the substrate using digital light processing technology, followed by PFOC acylation. The superhydrophilic-superhydrophobic patterned porous polymer film contains arrayed superhydrophilic microreaction units, separated by superhydrophobic polymer regions. Each superhydrophilic microreaction unit encapsulates an independent microdroplet, and the molecular logic circuitry is encapsulated within each independent microdroplet. This invention, through the combination of droplet microarray chip and molecular logic circuitry, achieves systematic improvements in multi-enzyme synergistic detection capabilities, logic computation complexity, spatial isolation control, high-throughput analysis, and adaptation to biological environments.

The invention discloses a sub-wavelength metal wire grating microarray polarization parameter reconstruction method and device, a medium and equipment. The method comprises the following steps: obtaining detection light intensity of a target in a fixed angle direction and detection light intensity change; obtaining polarization detection light intensity based on the sum value of the detection light intensity in each fixed direction and the detection light intensity change; obtaining a first target polarization detection vector matrix according to the Stokes parameter matrix; replacing the detection light intensity in the first target polarization detection vector matrix with the polarization detection light intensity to obtain a second target polarization detection vector matrix; and inputting the second target polarization detection vector matrix and a polarization transmittance parameter, an extinction ratio parameter and a uniformity parameter of the sub-wavelength metal wire grating microarray into a pre-constructed multi-parameter joint constraint polarization parameter reconstruction network, and outputting polarization energy, polarization degree and polarization angle information after target reconstruction. According to the invention, high-precision reconstruction of polarization energy parameters, polarization degree parameters and polarization angle parameters can be provided.

The invention relates to a method for microarray transformation, wherein, by using a cavity chip with transformation matrix, a template array can be copied onto a planar support, and the information or spatial arrangement is changed in the process, so that a transformed second array forms. The invention further relates to a device for carrying out such a method.

The present disclosure relates to the field of molecular biology and more specifically to microarrays and methods. In particular, the present disclosure relates to a method for amplifying a nucleic acid on a substrate comprising at least one well, the method includes: depositing a plurality of first capture primer pairs and a plurality of second capture primer pairs in a layer at least partially covering the inner well surface, wherein the primer density of first capture primer pairs is higher than the primer density of second primer pairs; contacting a sample comprising a plurality of target polynucleotides with the substrate under conditions sufficient for a single target polynucleotide per well to hybridize with a capture primer of the second capture primer pair; and performing a kinetic exclusion assay (KEA) to produce a monoclonal population of amplicons from the single target polynucleotide.