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Reaction plate and protein chip kit for integrated detection of multiple gynecologic tumor markers

A gynecological tumor and integrated detection technology, which is applied in the direction of analysis, measuring devices, and scientific instruments through chemical reactions of materials, can solve the problems of inability to meet the requirements of health checkups and clinical diagnosis, and high prices.

Inactive Publication Date: 2006-12-20
汪宁梅
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0014] The serum detection method of tumor markers is mainly based on ELISA method, which can only detect one indicator at a time, and is expensive, which cannot meet the comprehensive and efficient health examination and clinical diagnosis requirements of women.

Method used

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  • Reaction plate and protein chip kit for integrated detection of multiple gynecologic tumor markers
  • Reaction plate and protein chip kit for integrated detection of multiple gynecologic tumor markers

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] Preparation of detection reaction plate

[0045] Take a 96-well plate and set 42-84 sample wells and 1-6 standard wells. A solid phase support (NC membrane or PDVF membrane) is placed at the bottom of each reaction well, and the solid phase support is coated with anti-CEA, anti-CA15-3, anti-CA125, anti-SCC, HPV, anti-β-HCG, Anti-AFP and other seven kinds of antigen or antibody protein microarray ( figure 1 ).

Embodiment 2

[0047] kit preparation

[0048] Prepare a test kit for the integrated detection of seven indicators of a variety of gynecological tumors, which contains a reaction well plate (8-200 people) in Example 1, 1 bottle of washing concentrate, 1 bottle of enzyme-labeled working solution, and detection solution A One bottle, one bottle of detection solution B, one bottle of standard product.

[0049] Washing Concentrate, Enzyme Label Working Solution, Detection Solution A, Detection Solution B

Embodiment 3

[0051] Integrated detection of seven indicators of various gynecological tumors

[0052] Test each blood sample according to the following method:

[0053] 1. Add test blood or standard products to corresponding reaction wells, 100ul / well.

[0054] 2. Add enzyme-labeled working solution, 50ul / well.

[0055] 3. Incubate and shake at 30°C for 60 minutes.

[0056] 4. Wash 4-6 times with lotion, 5 minutes each time, vibrate.

[0057] 5. Add detection solution 40ul / well, so that the detection solution is evenly distributed on the bottom of the well.

[0058] 6. CCD detection, exposure for 3-20 seconds depending on the intensity of the luminescent signal.

[0059] Result judgment:

[0060] According to the CutOff value of different indicators, refer to the gradient curve of the standard to determine the negative or positive of the sample serum.

[0061] At the same time, the serum samples tested were routinely tested by conventional methods. The result shows that the test res...

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Abstract

The invention discloses an integral detecting reacting board and protein chip agent box of Carcinoembryonic antigen,CEA, 15-3 CA15-3(Carbohydrate Antigen 15-3,CA15-3), CA 125 (Cancer antigen 125,CA125), Squamous cell carcinoma antigen,SCC, Human papillomavirus antibody,HPVAb, beta-human chorionic gonadotropin, beta-HCG and alpha-fetoprotein,AFP, wherein the reacting hole board contains base and reacting hole on the base; the reacting hole contains 2-384 sample holes, 2-200 standard sample holes; the fixing phase carrier is set on the bottom of each reacting hole, which is covered by seven antigen or antibody molecule arrays with anti-CEA, anti-CA15-3,anti-CA125,anti-SCC,HPV,anti- beta-HCG,anti-AFP.

Description

technical field [0001] The invention belongs to the field of in vitro clinical testing and biochip technology, specifically, it is a seven-item indicator (CEA, CA15-3, CA125, SCC, anti-HPV, β -HCG, AFP) integrated detection reaction plate and kit Background technique [0002] In recent years, with the development of the economy and the improvement of people's living standards, the morbidity and mortality of some gynecological tumors such as cervical cancer and ovarian cancer have risen rapidly, women's health has been seriously threatened, and in addition to common gynecological tumors Breast cancer has become the malignant tumor with the highest incidence rate among women in the world, and it is one of the main causes of death among women. Therefore, it has important social and clinical significance to strengthen the early diagnosis of various female tumors. [0003] Tumor markers refer to substances secreted or shed by tumor cells into body fluids or tissues, or substance...

Claims

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Application Information

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IPC IPC(8): G01N33/544G01N33/533G01N21/31G01N21/76
Inventor 汪宁梅
Owner 汪宁梅
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