152 results about "Biochip" patented technology

The invention discloses a liquid drop routing method under fluid constraint in a digital micro-fluidic biochip based on reinforcement learning and imitation learning, and aims to solve the technical problems of low planning efficiency, low success rate and weak generalization ability of a traditional liquid drop routing algorithm under fluid constraint. The method adopts a strategy combining imitation learning and reinforcement learning and comprises the following steps: firstly, generating expert data by utilizing an expert algorithm meeting fluid constraint after transformation, and pre-training a network model through imitation learning to enable the network model to quickly grasp a basic routing strategy; the model and environment interaction are autonomously optimized through reinforcement learning, and the adaptability to the dynamic environment is improved. And finally fusing the weights to form a final model. Through the hybrid training framework, the success rate and planning efficiency of droplet routing are remarkably improved, and the generalization ability of the model to chip environments with different sizes is enhanced.

An integrated slide, a liquid changing device, a biochemical substance analysis system and an analysis method.The integrated slide comprises a biochip and an ink jet chip packaged above the biochip, the biochip and the ink jet chip define a biochemical reaction cavity, the ink jet chip is communicated with the biochemical reaction cavity, and the biochemical reaction cavity is communicated with the liquid changing device. Reagents required by biochemical reaction can be added into the biochemical reaction cavity, or reacted residual reagents in the biochemical reaction cavity can be discharged; and the biochip can realize in-situ signal detection. According to the biochemical substance analysis system applying the integrated slide glass, integrated operation of sample adding, biochemical substance analysis and imaging can be realized, the system structure is simplified, the reagent loss is reduced, the detection efficiency is favorably improved, and the detection cost is reduced.

The invention relates to biomedical engineering, and discloses a biochip screening method of a therapeutic agent based on intestinal brain axis regulation and application thereof. The screening method comprises the following steps: performing intestinal wall cell culture or microglial cell culture by adopting a metasurface plasmon resonance biochip of which the surface is subjected to bionic treatment to obtain an intestinal wall cell adherent chip board or a microglial cell adherent chip board; the method comprises the following steps: adding a culture medium containing a chemotherapeutic drug or lipopolysaccharide into a cell-adherent chip board for culture I, then removing the culture medium, adding a therapeutic agent to be detected for culture II to obtain a culture solution, and detecting the cell repair effect of intestinal wall cells or microglial cells in the culture solution, and screening to obtain a therapeutic agent with a repairing effect on intestinal wall cells and / or microglial cells. The screening method can be used for efficiently and accurately screening the therapeutic agent with dual effects of intestinal regulation and neuroprotection from a plurality of therapeutic agents to be detected, and can be used for dynamically monitoring and exploring the interaction condition of the therapeutic agent and cells on line in an unmarked and real-time manner.

Embodiments of the present invention provide a sensing apparatus. The sensing apparatus includes a biochip unit and a receptacle device. The biochip unit includes a reservoir configured to receive a predetermined amount of an analyte, a plurality of working electrodes configured to be in contact with the analyte, a reference electrode and a counter electrode configured to complete an electrical circuit with the plurality of working electrodes. The receptacle device includes a receptacle unit configured to be electrically and mechanically coupled to the biochip unit, a function generator electrically coupled to the receptacle unit, a memory unit configured to store machine-readable instructions, and a processor operably connected to the memory unit. The processor is enabled by the machine-readable instructions to determine a plurality of deduced values corresponding to a plurality of analyte parameters of the analyte from the plurality of observed values of the plurality of predetermined parameters.

The invention relates to a detection method, in particular to a method for rapidly detecting alpha and beta-lactoglobulin in auxiliary material lactose based on a microarray chip technology. On the basis of an indirect competitive enzyme-linked immunosorbent assay principle and a silver enhanced developing technology, a high-sensitivity microarray chip detection platform is successfully constructed by virtue of a biological chip scanner, and rapid quantitative analysis of alpha-lactalbumin and beta-lactoglobulin in the auxiliary material lactose for injection is realized. According to the method, an antigen-antibody specific recognition mechanism and a nano-silver signal amplification mechanism are integrated, and the detection efficiency and sensitivity are remarkably improved through the high-throughput layout of the microarray chip. In the aspect of methodology performance, the technology has the remarkable advantages of simple and convenient operation process, short detection time, high sample treatment flux, excellent specificity (CRlt; 1%) and the like.

An automatic sample adding and detecting device for a biological chip relates to the technical field of biological chip detection and comprises a platform, a coordinate positioning mechanism, a supporting plate, a biological chip, a sealing plate, a detection hole, an automatic buffer solution feeder and a biological sample feeder, and the automatic buffer solution feeder and the biological sample feeder are identical in structure. Each feeder comprises a feeder body and a driving mechanism used for controlling the feeder body to quantitatively discharge liquid, a support is further fixedly arranged at the top end of the platform, an optical fiber probe is arranged on the support, and a shooting camera is arranged on the support on one side of the optical fiber probe. The liquid adding device does not need frequent sampling, can ensure that the liquid adding amount each time is consistent, avoids the tedious structure and operation of repeated sampling, promotes the detection result to be more accurate due to the uniform liquid adding amount, and is simple in structure, convenient to use, low in manufacturing cost, convenient to maintain and suitable for popularization and use.

The utility model relates to the technical field of biochip manufacturing equipment, in particular to a membrane uncovering mechanism for a membrane, which comprises a working table, a sliding frame and a clamping jaw assembly, the working table is provided with a waste material port, the sliding frame is horizontally and slidably mounted on the working table through a sliding component, and the clamping jaw assembly is arranged on the working table. The clamping jaw assembly is installed on the sliding frame in a lifting mode through a lifting assembly, a clamping jaw used for uncovering a film is arranged below the clamping jaw assembly, and after the film is uncovered, the clamping jaw horizontally slides and moves to the waste material opening through the sliding frame to discard the PDMS layer. According to the film uncovering mechanism, through cooperation of the sliding assembly, the lifting assembly, the clamping jaw assembly and the like, automatic operation of the film uncovering process can be achieved, the film uncovering efficiency is improved, and damage to the substrate is avoided.

A method for analyzing a biochip image is provided, including: (S1) acquiring and preprocessing the biochip image to obtain a preprocessed image; (S2) performing a correction for angle deflection on the preprocessed image to obtain a deflection-corrected image; and (S3) performing an enhancement processing on the deflection-corrected image, and identifying a positive or negative of an area of interest in the preprocessed image according to an image on which the enhancement processing has been performed. An apparatus (100) for analyzing a biochip image, a method for analyzing an image, a computer device (200) and a storage medium are disclosed.

A sample loading device and a gene sequencer, the sample loading device comprising: a fixed seat assembly, a gland assembly and a cap assembly, the fixed seat assembly having a fixed position for placing a slide frame loaded with a biochip; the gland assembly is rotatably connected to the fixed seat assembly, and the gland assembly is further snap-connected with the fixed seat assembly to fix the slide frame in the fixed position, and the gland assembly is provided with a positioning hole for installing a pipette head; the cap assembly is detachably arranged on the gland assembly and covers the positioning hole, and the cap assembly is used for pressing the pipette head in the positioning hole to provide a pushing force for the liquid in the pipette head. The sample loading device of the present application can realize the fixation of the slide frame by the gland assembly and the fixed seat assembly, and the cap assembly arranged on the gland assembly can provide a pushing force for the liquid in the pipette head, promote the flow of the internal liquid, and be beneficial to the automatic liquid passing of the biochip.

The invention relates to a synchronous detection method and system for small molecules and protein, and the synchronous detection method comprises the following steps: respectively preparing a small molecule detection antibody and a protein detection antibody marked by carboxyl fluorescent microspheres, and mixing the small molecule detection antibody and the protein detection antibody to obtain a mixed detection antibody; respectively preparing a microsphere-labeled capture micromolecule antigen and a protein antibody; preparing a microsphere array biochip with two detection areas; carrying out sample application on the microsphere-labeled captured micromolecule antigen and the protein antibody to the two detection areas; mixing a sample to be detected with the mixed detection antibody according to different proportions; slowly adding the sample mixed solution into two detection areas of the microsphere array biochip respectively; scanning and imaging the two detection areas of the microsphere array biochip respectively, identifying all fluorescent spot information in a fluorescent image, and obtaining the concentration of small molecules and proteins in a sample to be detected through a Poisson distribution principle or by establishing a standard curve. According to the invention, synchronous detection of small molecules and proteins can be realized.

The application provides a disc-based multi-staining biochip detection method based on nanoparticle disturbance laser signal, which comprises the following steps: S1, determining staining substances of various to-be-detected organisms according to the concentration detection range of various to-be-detected organism solutions; S2, preparing a staining organism array of various to-be-detected organism solutions on a disc base to obtain a disc-based staining biochip; S3, scanning the disc-based staining biochip based on a blue light drive; and S4, analyzing the BIS error number at each logical position in the scanning result, and determining the concentration of the to-be-detected organism solution according to the BIS error number size. The application can realize the simultaneous detection of the concentrations of various organism solutions, and improves the detection efficiency.

The present application relates to the technical field of biochip, in particular to a multi-waveband synergic release permeation-quantitative biochip, which comprises a substrate, a permeation function layer and a gold plating layer stacked from bottom to top; the substrate is made of graphene material with a thickness of 0.05-0.1mm, used for receiving and storing environmental energy; the permeation function layer has a thickness of 0.1-0.3mm, which is made of a mixture of nano far infrared powder, anion powder, magnetic powder and nano catalytic material with a mass ratio of 3:2:2:1, and then compounded with a biocompatible adhesive; the present application simultaneously releases magnetic wave, far infrared wave, anion wave and quantitative wave, and has the dual functions of human physiotherapy and material purification, solves the problem of single function of the existing products, the energy storage capacity of the graphene substrate and the energy aggregation effect of the gold plating layer greatly improve the energy utilization efficiency, prolong the chip action time, the permeation technology strengthens the waveband penetration capacity, and when acting on the human body, it does not need to be pasted on the skin, and when acting on the material, it does not need to be soaked, and the use scene is flexible.

The application provides a continuous microfluidic biochip underflow layer physical collaborative design method, which realizes the underflow layer physical design work in three stages.(1) Logic design stage: through the first proposed logic layout and component direction layout adjustment method, the excellent logic position and logic direction of the component are obtained, which greatly improves the efficiency while ensuring the high-quality layout solution.(2) Component mapping and bounding box gap layout adjustment stage: based on the bounding box strategy, the component logic design result of the previous stage is mapped to the actual physical design space, and the best bounding box gap is obtained through the bounding box gap layout adjustment.(3) Shrink layout adjustment stage: based on the connection graph relationship between components, two new layout adjustment methods, namely along-flow channel shrinkage and multi-graph shrinkage, are proposed, which further efficiently improves the quality of the underflow layer physical design. The application not only effectively improves the quality of the underflow layer physical design, but also greatly improves the efficiency.

The application discloses a kind of based on nano-silver-porous silicon simultaneously SERS detection variety of mycotoxin biochip sensor and its preparation method and application, the sensor includes SERS substrate and SERS label two parts compositions, SERS substrate is by porous silicon surface magnetron sputtering on silver nanoparticles and silver nanoparticles surface connection three kinds of mycotoxin artificial antigen composition, SERS label is connected with nile blue and corresponding mycotoxin antibody gold nanoparticles.The application can be combined by each mycotoxin and porous silicon silver surface mycotoxin artificial antigen mycotoxin antibody on SERS label, by detecting the Raman signal of NBA on SERS label surface, realize three kinds of mycotoxin simultaneous quantitative analysis, detection speed is fast, sensitivity is high, while various toxins in gualou, malt and kudzu root recovery rate is all greater than 75%, the coefficient of variation of same batch is less than 5.2%, and the coefficient of variation of different batches is less than 7.8%.

The application provides a continuous micro-fluidic biochip high-level synthesis method considering volume management, is based on a discrete particle swarm algorithm with high solving efficiency, and comprises the following key technologies: 1) a particle coding scheme suitable for the high-level synthesis problem considering volume management is provided; 2) an automatic scheduling scheme generation method is provided, which can accurately generate a corresponding operation scheduling scheme according to the particle coding; and 3) according to the characteristics of the problem, a corresponding particle updating strategy is designed, and the convergence of the algorithm is accelerated. The application can obtain a high-level synthesis scheme satisfying volume constraints and having high quality in a shorter time.

The application discloses a small-molecule multi-connection detection kit based on a biochip, a preparation method and a detection method thereof. The kit comprises nano-gold particle labeled antibodies, a multi-connection detection chip plate and a detection buffer. The nano-gold particle labeled antibodies comprise nano-gold particle labeled florfenicol antibodies and enrofloxacin antibodies. The multi-connection detection chip plate is coated with florfenicol antigens and enrofloxacin antigens in detection holes. The detection buffer is composed of 10-40 mM phosphate buffer and 0.05-0.5 wt% surfactant S17. The application can simultaneously detect florfenicol and enrofloxacin small molecules by using one detection buffer, thereby saving time and labor.

The utility model discloses an autoimmune liver disease antibody detection chip, which belongs to the technical field of biochips and diagnostic reagents and comprises a reaction membrane, an upper shell and a lower shell, the upper shell and the lower shell can be buckled together, a water absorption layer is arranged between the upper shell and the lower shell, a blood filtering component is movably arranged above the upper shell, and a water outlet is formed in the lower shell. The blood filtering assembly comprises a blood filtering cup and a blood filtering film, and the blood filtering film is fixedly connected with the blood filtering cup. According to the autoimmune liver disease antibody detection chip disclosed by the utility model, the blood filtering film can be flexibly mounted and dismounted, and the waste of the blood filtering film is avoided.

The invention discloses a clockwork spring type flexible feeding device which comprises a bearing bin used for bearing a biological chip; the transfer assembly comprises a flexible transfer piece, and a clamping head is arranged at the end of the flexible transfer piece and used for clamping and transferring the biological chip; the rail transfer device comprises a plurality of rail transfer blocks, and a plurality of rails are arranged in the rail transfer blocks. The device has the beneficial effects that the flexible conveying piece is arranged, conveying of the biological chips is achieved, compared with a traditional rigid transmission structure, the occupied space of the device is remarkably reduced through curling storage, and the device is especially suitable for the internal layout of biological medicine detection equipment with the high requirement for the integration level; the efficient, stable and compact transfer of the biological chip in a complex detection process is realized.

The invention belongs to the technical field of automatic equipment of biological chips, and particularly relates to a bearing assembly for biological chips and a sample application mechanism, the bearing assembly comprises a base, a supporting plate and an alignment plate, the supporting plate is provided with a plurality of storage grooves for storing the biological chips, the edge of each storage groove is provided with a through hole, and the alignment plate is provided with a plurality of through holes; a plurality of clamping blocks are arranged on the alignment plate, the clamping blocks are located in the through holes in a one-to-one correspondence mode, and the alignment plate moves to drive the clamping blocks to clamp or loosen the biological chips in the storage grooves. The bearing assembly can guarantee that the biological chips can be conveniently and completely taken and placed, and the biological chips can be clamped and aligned through the alignment plate, so that subsequent sample application and other processes are facilitated. According to the sample application mechanism, the bearing assembly is adopted, the biological chips can be rapidly and conveniently clamped and aligned, it is guaranteed that the relative positions of micro-channel inlets of all the biological chips on the supporting plate are the same, antibodies can be accurately added by aligning the inlets during sample application, and the sample application efficiency is greatly improved.

Described are wastewater treatment systems comprising a biologically active conduit defining a single, confined serpentine flow path twisting from top to bottom portions. The system establishes sequential aerobic, anoxic, and anaerobic treatment zones populated with zone-specific biological chips containing dominant biologically active media. Mesh separators prevent biological chip displacement between zones while allowing wastewater flow. The system receives influent wastewater through a bottom opening and dispenses treated effluent from the same opening following simultaneous batch treatment across all zones. Methods include wastewater treatment processes, modular assembly techniques, and attribute management systems using adjustable bypass valves and venturi-based air introduction for precise dissolved oxygen control. The systems achieve enhanced PFAS removal by reducing organic matter competition for granular activated carbon (GAC) adsorption sites, extending GAC operational life by at least 25%. Advanced features include dual recirculation capabilities, overflow protection, space-efficient modular design, and fine-tuned control enabling >90% PFAS removal efficiency.

This invention discloses a control logic routing method for a fully programmable valve array biochip, used to automate the channel routing of FPVA control logic. The method aims to minimize the time delay deviation between synchronized valves and minimize the control channel bus length. It implements control channel routing that considers line length matching for the logic architecture. The method uses a competitive deep dual-Q network as the agent of the DRL to achieve adaptive routing of channels in the control logic. The key parts of the framework, such as the state space, action space, and reward function, are designed based on the line length matching requirements of the control channels, thereby minimizing the time delay deviation of synchronized valves and the control channel bus length in the biochip.

The invention discloses a biological wave resonator of a far infrared band, which comprises a UHF PCB wafer resonance module body, and at least one group of double-loop circulating type miniature biological chip modules are arranged in the module body; the double-loop circulating type micro biological chip module comprises an FPC (Flexible Printed Circuit) base material copper etching receiving and transmitting antenna module, a resonance loop module and a processor module, and the processor module is integrated with a power supply conversion module and a signal output module. And in combination with a same-frequency resonance mechanism of the double-loop circulating type micro biological chip module, the cell biological magnetic field can be corrected from the cellular level, the cell activity can be activated, the blood circulation and metabolism of a human body can be improved, and physical fatigue and chronic disease discomfort can be relieved.

The utility model discloses a solid phase substrate and biochip. One surface of solid phase substrate is equipped with multiple interval arrangement marks, and each mark includes the first mark line along the first direction extension, and the second mark line along the second direction extension and the intersection with first mark line, and the end of first mark line and second mark line all are located in the edge of solid phase substrate. The utility model implementation's solid phase substrate, because the end of first mark line and second mark line all are located in the edge of solid phase substrate, multiple marks interval arrangement, compared with the mark line of through solid phase substrate, has reduced the area that mark has occupied on solid phase substrate surface, thereby reduced the flux loss of biochip in gene sequencing.

A composition and method for capturing and releasing cells containing CD44 antigen, comprising cholesterol-aptamers, silver nanoparticle-modified aptamers, and bleomycin-ferrous ion complexes, exhibits higher biocompatibility and stability, facilitating the formation of a biointerface for the efficient capture and release of mesenchymal stem cells. Applying the composition of this invention to the electrochemical detection of cell capture and release offers advantages such as mild reaction conditions, simplicity, speed, and sensitivity, making it easy to integrate with other automated reaction interfaces for commercial development. Furthermore, it can be combined with electrochemical biochip sensor arrays to achieve even more convenient capture, detection, and release of mesenchymal stem cells.

Provided are a microfluidic detection system for a refrigerator and the refrigerator. The microfluidic detection system comprises: a microfluidic biochip that has a sample inlet, a detection pool and a communication port, all of which communicate in sequence by means of a microfluidic channel; a sample liquid driving device in sealed communication with the communication port by means of a sealed docking mechanism; and a detection mechanism for detecting the detection cell to obtain preset detection parameters of a sample liquid. The communication port is fixedly provided with a plug pin having an internal flow channel in sealed communication with the communication port, the plug pin is inserted into and forms a fluid-tight connection with the sealed docking mechanism, and the sealed docking mechanism is in fluid-tight connection with the sample liquid driving device, so that the sample liquid driving device is in sealed communication with the communication port.

The present application relates to a kind of biochip and its manufacturing method and application.The biochip includes: substrate;Multiple pixel units are arranged on the substrate, and each pixel unit includes the microelectrode array with working electrode, counter electrode and dielectric structure;Wherein, the surface of the dielectric structure has surface modification layer, and the surface modification layer includes interlinked first surface modification structure and second surface modification structure;The first surface modification structure includes the alkoxysilane modification layer bonded with the surface of the dielectric structure and the functional modification layer connected with the alkoxysilane modification layer by sulfide bond;And the second surface modification structure includes the alkoxysilane modification layer bonded with the surface of the dielectric structure.The biochip surface has region-selective and functionally differentiated functional structure, and it is of great significance to promote the development of high-throughput, programmable and strong bio-compatible chip technology.

The application discloses an ultrahigh-sensitivity photonic crystal chip, a preparation method thereof and application of the photonic crystal chip in detection of CGRP. The photonic crystal biological chip comprises a photonic crystal structure and an immune detection system; the immune detection system is fixed on the photonic crystal structure, the immune detection system contains antibodies of a biological detection marker; and the photonic crystal structure is formed by self-assembly of photonic crystals on a base material. For detection of CGRP, a target object which is easy to degrade and has an ultralow concentration, the application provides a quick and simple detection method and an optical detection platform. Compared with ELISA detection which needs several hours of detection time, the detection platform of the application is simple to operate and quick in detection (only 10 minutes are needed).

A kind of biological chip data feature engineering method based on statistical machine learning, comprising the following steps: generating data matrix;Carrying out z-score standardization;Value is calculated, and large-value gene data is screened;Correlation coefficient matrix is generated;Screening gene pair;Complex correlation coefficient is calculated;Mark gene complex correlation coefficient change.The present application is beneficial to analyze the correlation between data in a large number of biological chip data, and select a certain number of genes reflecting the difference between data groups according to the demand by using the method of feature selection.The present application uses correlation analysis statistics correlation coefficient, partial correlation coefficient and complex correlation coefficient for feature selection, which is beneficial to further reduce the data dimension, and is beneficial to predict the correlation change between two genes under different experimental treatment conditions.

The utility model discloses a biological chip placing tray which comprises a base plate, and the upper end of the base plate is fixedly connected with a fixing frame; the splicing structure is arranged at the lower end of the base plate; the clamping grooves are formed in the two ends of the inner wall of the fixing frame; the adjusting structure is arranged on the inner side of the fixing frame; the limiting plate is clamped on the inner side of the clamping groove; the first cavity is formed in the base plate; the damping rod is fixedly connected to the lower end of the inner wall of the first cavity; and the buffer plate is fixedly connected to the upper end of the damping rod. Compared with the prior art, the biological chip has the advantages that the practicability of the biological chip can be improved; and biological chips with different sizes can be placed on the placing disc.

A system and method for diagnosing traumatic brain injury (TBI) includes a proteome biochip including a set of brain protein fractions including primary serum autoantibodies reactive with brain protein autoantigens released by the TBI printed into micro-wells of a glass slide. The biochip hybridized with non-TBI and TBI-injured serum samples from which an autoantibody response profile is generated. The system additionally including labeled IgG or IgM secondary antibodies for addition to the micro-wells for binding with one of the primary serum autoantibodies, a side illumination laser to read the micro-wells in which the labeled IgG or IgM secondary antibodies are bound with one of the primary serum autoantibodies, and a readout detection system for the set of brain protein fractions to screen for autoantigens present in the micro-wells that contain the labeled IgG or IgM secondary antibodies bound with one of the primary serum autoantibodies to generate a heat map.