455 results about "Biochip" patented technology

The invention discloses a livestock health state real-time monitoring method and system based on the Internet of Things, and the method comprises the steps: collecting livestock biological data and environment parameters through a biological chip ear tag and an environment sensor, and carrying out the preprocessing, and obtaining a standardized multi-dimensional livestock data flow; in combination with image data provided by a video monitoring system, identifying livestock individuals and analyzing behavior features, and fusing biological data and visual data to generate livestock individualized feature vectors; constructing an individualized health baseline model in combination with historical health records; comparing the deviation degree between the current state and the health baseline through an anomaly detection algorithm, and identifying a potential health problem; early warning is performed according to the abnormity severity level, intervention suggestions are generated, early warning information is pushed to the breeding personnel through multiple channels, and response measures and results are recorded to form closed-loop management. According to the invention, accurate real-time monitoring of the health state of the livestock is realized, the accuracy and timeliness of anomaly detection are improved, the breeding risk is reduced, and the production efficiency of animal husbandry is improved.

The invention discloses a liquid drop routing method under fluid constraint in a digital micro-fluidic biochip based on reinforcement learning and imitation learning, and aims to solve the technical problems of low planning efficiency, low success rate and weak generalization ability of a traditional liquid drop routing algorithm under fluid constraint. The method adopts a strategy combining imitation learning and reinforcement learning and comprises the following steps: firstly, generating expert data by utilizing an expert algorithm meeting fluid constraint after transformation, and pre-training a network model through imitation learning to enable the network model to quickly grasp a basic routing strategy; the model and environment interaction are autonomously optimized through reinforcement learning, and the adaptability to the dynamic environment is improved. And finally fusing the weights to form a final model. Through the hybrid training framework, the success rate and planning efficiency of droplet routing are remarkably improved, and the generalization ability of the model to chip environments with different sizes is enhanced.

The invention discloses a recyclable biochip stationary phase as well as a preparation method and application thereof, and belongs to the field of biochips. The preparation method of the biochip stationary phase comprises the following steps: (1) activating a glass slide by adopting plasma, and then putting the glass slide into a silane coupling agent solution to react, so that a silane coupling agent is covalently bound with the glass slide to obtain a functionally modified biochip; and (2) combining the 5'end modified single chain oligo with the functionally modified biochip to obtain the biochip stationary phase. The biochip stationary phase disclosed by the invention is low in cost, excellent in physicochemical property, good in stability and tolerance and capable of adapting to a complex environment; more importantly, the method can be recycled, the resource utilization rate is increased, the pollution is reduced, and the method has huge application potential in the leading technical field of DNA synthesis by an enzyme method and the like.

The invention belongs to the technical field of digital micro-fluidic chip design. The invention provides a droplet group wiring method of an active pixel array digital micro-fluidic biochip. According to the embodiment of the invention, the initial position of a liquid drop is obtained by using a liquid drop spacing adjustment algorithm; secondly, obtaining preset paths of all liquid drops by utilizing a liquid drop preset path searching method; and finally, a droplet group wiring result is obtained by using a concurrent wiring algorithm. On the other hand, according to the method, the maximum number of the liquid drops moved at the same time is almost the same as the number of the liquid drops, efficient wiring of large-scale liquid drop clusters on the active pixel array digital micro-fluidic biochip can be completed under the condition that the CPU operation time is controlled to be 1 s, and the wiring efficiency is greatly improved at the latest arrival time. The average arrival time can show the superiority of the method. According to the method, the resource of the available area of the chip can be utilized to the maximum extent, and the wiring failure condition caused by the conflict of the liquid drops can be efficiently treated.

An integrated slide, a liquid changing apparatus, and a biochemical substance analysis system and analysis method. The integrated slide comprises a biochip and an ink-jet chip packaged above the biochip, the biochip and the ink-jet chip defining a biochemical reaction chamber; the ink-jet chip is communicated with the biochemical reaction chamber and is capable of adding into the biochemical reaction chamber a reagent needed by a biochemical reaction, or discharging from the biochemical reaction chamber a residual reagent which has undergone a reaction; the biological chip can implement in-situ signal detection. In the present application, the biochemical substance analysis system using the integrated slide can implement an integrated operation of sample addition, biochemical substance analysis and imaging, thereby simplifying the system structure, reducing reagent losses, helping to improve detection efficiency and reducing detection costs.

The invention relates to the technical field of drug toxicity evaluation, in particular to an evaluation method for examining toxic and side effects of drugs through a biological organ model. The method comprises the following steps: pretreating a cell line, and carrying out mixed culture with a skeleton structure; connecting an organ model through a biochip; designing a micro-channel network to form a circulation simulation system, and adjusting micro-environment parameters; embedding a biosensor into the biological organ model, and collecting data; adding drugs with different concentrations, and evaluating according to data. According to the method, firstly, the liver, the kidney and the heart are constructed, then the three organ models are connected through the microfluidic technology, microenvironment factors such as micro blood vessels and lymphatic vessels are introduced into the connecting positions, finally, the biosensor technology is introduced, the cell state, metabolites and microenvironment parameters in all the organ models are monitored in real time, and the three organ models are obtained. Through the multi-dimensional biological organ model, the influence of the medicine on different organs can be evaluated more comprehensively.

This invention belongs to the field of biochip technology, specifically an erasable single nucleotide polymorphism (SNP) genotyping chip and its detection method. The erasable SNP genotyping chip provided by this invention is prepared using polystyrene microspheres, capture probes, and a silicon substrate. In the SNP recognition stage, it relies on a FEN1 enzyme-mediated invasive reaction. Based on the technical characteristics of this invasive reaction, downstream probes A and B are designed with self-protective hairpin structures, preventing false positive signals from hybridization with the capture probe even when added in excess, thus significantly improving detection specificity. The detection method of the erasable SNP genotyping chip provided by this invention overcomes the long-standing problem of high application costs caused by the single-use nature of solid-phase SNP gene detection chips.

The invention discloses a universal biochip as well as a preparation method and application thereof. The preparation method of the universal biochip comprises the following steps: S1, soaking a substrate in a solution consisting of a silane reagent A, an alkaline reagent and a buffer salt solution for reaction, and curing to form a silicon dioxide film on the surface of the substrate; the material of the substrate is selected from any one of glass, metal, plastic, resin and a film material; s2, soaking the substrate treated in the step S1 in a solution of a silane reagent B connected with an active group for reaction so as to modify the active group on the surface of the silicon dioxide film; and S3, carrying out a coupling reaction on the surface active group of the substrate treated in the step S2 and a DNA probe or a group in protein to obtain the universal biochip. The method is suitable for various metal and non-metal material substrates, the preparation process is simple, the condition is mild, the biological chip can be processed into the required shape according to the material, and the application range of the biological chip is further expanded.

The utility model relates to the technical field of biological chips, in particular to a multi-tissue co-culture organoid chip which comprises a chip main body, and a plurality of culture hole groups are formed in the chip main body; the culture hole group comprises a first cell culture hole and at least one second cell culture hole communicated with the first cell culture hole, and a porous membrane for cells to pass through is arranged at the communicated part of the first cell culture hole and each second cell culture hole; the mode that the two cell culture holes are communicated and separated through the porous membrane is adopted, organoid co-culture is achieved, meanwhile, the pathological and physiological dynamic microenvironment of tumor tissue can be simulated, the operation process of medicine screening is simplified, and the medicine screening efficiency is greatly improved.

The invention provides a sample application method and a sample application device for a biological chip. The sample application method for the biological chip comprises the following steps: placing a biological molecule solution with a preset volume at a preset position on a solid-phase support in a contact or non-contact manner to form a sample application point array; wherein the sample application point array comprises a plurality of sample application points which are uniformly or basically uniformly distributed, each sample application point comprises a tiny sample application point group, and each tiny sample application point group comprises a plurality of tiny sample application points which are uniformly or basically uniformly distributed so as to inhibit the coffee ring effect. According to the biological chip spotting method provided by the invention, a single spotting point is decomposed into a plurality of tiny spotting points, so that the problem that the signal uniformity of a detection site is influenced by a coffee ring effect is solved, the detection signal is more uniform, and in addition, the detection point signal can be accurately determined after the coffee ring effect is overcome, so that quantitative analysis can be realized; and the application scene of the biochip is expanded.

A biochip for use in detecting biological materials is provided. The semiconductor device includes a substrate (110), semiconductor layers (120, 121, 122, 123), an insulating layer (130), IL1, IL2, metal layers (140, 141, 142, 143), a solution (200, 210, 220), a first protective layer (150, 152, 153) and a second protective layer (160, 161, 162, 163). The semiconductor layer is disposed on the substrate and has a reaction region. The insulating layer is disposed on the semiconductor layer and has a first opening (O1) exposing the reaction region. The metal layer is disposed on the insulating layer (130). The metal layer includes a source electrode (141), a drain electrode (142) and a surrounding wall structure (143). The source electrode and the drain electrode are electrically connected to the semiconductor layer, respectively. The surrounding wall structure has a first opening (O1). The opening surrounds the source electrode and the drain electrode. A first protective layer is disposed on the metal layer and has a second opening O2. A second protective layer is disposed on the first protective layer and has a third opening O3 and a fourth opening O4. The second protective layer includes a first protrusion 161, a second protrusion 162 and a third protrusion 163. The first protrusion is disposed corresponding to the source electrode, the second protrusion is disposed corresponding to the drain electrode, and the third protrusion is disposed corresponding to the surrounding wall structure, surrounding and defining the fourth opening. A solution containing bioprobes 210 is disposed in the opening. In the normal direction of the substrate, the third opening overlaps the second opening and the first opening, and the fourth opening exposes the first protective layer.

The invention belongs to the technical field of biochips, and particularly relates to a reusable genetic typing chip and a detection method thereof, the reusable genetic typing chip provided by the invention is prepared from polystyrene microspheres, capture probes and a silicon substrate, and depends on competitive hybridization of DNA in an SNP recognition stage, and the detection result is more accurate. The recognition sequence of the capture probe is converted into the sequence of the target object, and the added detection probes are hairpin probes with a self-protection function, so that the generation of false positive signals is remarkably reduced, and the detection specificity is remarkably improved. And no enzyme participates in the whole process, so that the detection process is remarkably simplified, and the detection cost is reduced.

The invention provides an FPVA biochip layout method considering reagent type difference in unit multiplexing, which comprises the following steps of: modeling a biochip layout task as a Markov decision process: according to the size of an input FPVA chip, the dependency relationship of each operation in a biochemical reaction sequence diagram, the reagent type contained in each operation and a binding scheduling scheme, determining the FPVA biochip layout task; initializing an FPVA mesh model and defining a state space, an action space and a reward function; wherein the reward function is set according to the total unit multiplexing complexity of the current layout scheme, the legality of the current component layout and the distance between the component and the center point of the FPVA chip; and finally, completing the FPVA biochip layout through the dual deep Q network based on a Markov decision process. By designing a reward function, the unit multiplexing complexity is minimized, and the distance between components containing the same type of reagents is reduced, so that the purposes of minimizing the cross contamination degree of a final layout scheme, the total length of a fluid transportation path and the bioassay completion time are achieved.

Devices, methods for fabricating said devices, and methods for detecting an analyte within a bio-target are described herein. The device includes a top assembly and a bottom assembly. The Top assembly includes an electrode disposed on a top layer. The bottom assembly includes a bio-chip disposed on a bottom layer and a polymer body disposed between the bio-chip and the top assembly. The polymer body includes a channel. The electrode of the top assembly is positioned within the channel. The channel is configured to accommodate the bio-target containing the analyte.

A biochemical substance analysis system and a biochemical substance analysis method, the biochemical substance analysis system comprises a biochemical reaction module, a liquid change module, an imaging detection module, a transfer module and a stage, a biochip is located on the stage, and the transfer module drives the stage to be movably arranged relative to the biochemical reaction module, the liquid change module and the imaging detection module; the liquid changing module can print a reaction layer with a preset thickness on the biological chip; a reaction control device in the biochemical reaction module and the carrying table can form a reaction chamber for reaction of a sample to be detected and a reagent; and the imaging detection module can perform imaging acquisition on the reacted sample to be detected. The biochemical reaction module, the liquid change module, the imaging detection module and the transfer module are integrated in one system, so that the integrated operation of sample adding, biochemical substance analysis and imaging can be realized, the system structure is simplified, the reagent loss is reduced, the biochemical detection efficiency is improved, and the biochemical detection cost is reduced.

The invention relates to the technical field of veterinary epidemic disease detection, and discloses a veterinary epidemic disease remote monitoring and intelligent diagnosis system, which comprises a multi-mode biosensor array including an implantable miniature biochip, a wearable flexible physiological monitoring patch and an environment sensing sensor cluster, the implantable miniature biochip is used for being implanted into a key tissue organ area in an animal body and collecting microscopic physiological data such as cellular metabolism signals and local immune reaction marker concentration in real time, and the wearable flexible physiological monitoring patch is used for being attached to the body surface of the animal. By means of the multi-mode biosensor array and the edge intelligent preprocessing unit, animal physiology and environment data are accurately monitored in real time. The multi-modal biosensor array covers the implantable miniature biochip, the wearable flexible physiological monitoring patch and the environment sensing sensor cluster, and can comprehensively collect in-vivo microscopic physiological data, body surface macroscopic physiological parameters and feeding environment information of animals.

The invention discloses a reduced graphene oxide biochip based on a piezoelectric substrate and a preparation method and application thereof, the reduced graphene oxide biochip based on the piezoelectric substrate comprises a sound transduction unit and at least one detection unit connected to the sound transduction unit, and a microfluid channel layer is arranged between the sound transduction unit and the detection unit. A reduced graphene oxide layer is directly formed on the surface of a piezoelectric layer of a detection unit, a biological recognition element is fixed on the reduced graphene oxide layer, and the biological recognition element is used for being combined with target biological molecules to generate a reaction product with electron donating and receiving capacity. On the basis, the acoustic transduction electrode design of a concentric interdigital electrode structure is adopted, and the acoustic surface wave intensity which is more concentrated compared with other electrode structures is generated by utilizing the focusing effect of the concentric electrode structure; the flow velocity of the fluid to be measured in the microfluid channel is faster, and the directivity is stronger and more concentrated.

An integrated slide, a liquid changing device, a biochemical substance analysis system and an analysis method.The integrated slide comprises a biochip and an ink jet chip packaged above the biochip, the biochip and the ink jet chip define a biochemical reaction cavity, the ink jet chip is communicated with the biochemical reaction cavity, and the biochemical reaction cavity is communicated with the liquid changing device. Reagents required by biochemical reaction can be added into the biochemical reaction cavity, or reacted residual reagents in the biochemical reaction cavity can be discharged; and the biochip can realize in-situ signal detection. According to the biochemical substance analysis system applying the integrated slide glass, integrated operation of sample adding, biochemical substance analysis and imaging can be realized, the system structure is simplified, the reagent loss is reduced, the detection efficiency is favorably improved, and the detection cost is reduced.

A bio-chip is provided. The bio-chip includes a first substrate, a polarizing array, and a plurality of reaction sites. The polarizing array is disposed on the first substrate, and includes first sub-polarizing units having a first polarizing angle and second sub-polarizing units having a second polarizing angle. The first polarizing angle is different from the second polarizing angle. The reaction sites are disposed on the polarizing array. Each of the reaction sites corresponds to one of the first sub-polarizing units or one of the second sub-polarizing units.

The present invention provides an information processing method, device, equipment and storage medium based on cell segmentation, which relates to the field of biological information technology. The method includes: obtaining a corrected scanned image; the scanned image is obtained based on a tissue slice attached to a biochip; performing cell segmentation on the scanned image to obtain a cell segmentation result; based on the inherent position of each barcode microsphere in the biochip, extracting the position information of the barcode microsphere corresponding to each cell in the cell segmentation result; based on the position information of the barcode microsphere corresponding to each cell in the cell segmentation result, determining the first position information of each cell and the statistical information of the corresponding barcode microsphere. The present invention can improve the efficiency and accuracy of cell analysis.

The invention discloses a biological chip for simultaneously detecting multiple abundance markers and application thereof, and belongs to the technical field of immunodetection. The biochip comprises a PDMS layer, a glass substrate layer, a detection channel formed between the PDMS layer and the glass substrate layer, and a chip inlet and a chip outlet which are communicated with the detection channel, the detection channel comprises a CA19-9 detection channel, a CA125 detection channel and an LIF detection channel; the CA19-9 detection channel and the CA125 detection channel are filled with capture antibody modified immunomagnetic beads and immunofluorescence modified detection antibodies; the LIF detection channel is filled with an immunofluorescence modified detection antibody, and the surface of the glass substrate layer in the LIF detection channel is modified with an Anti-LIF capture antibody. The invention discloses a biochip for simultaneously detecting multiple abundance markers with high capture concentration and application of the biochip.

The invention belongs to the technical field of biochips, and relates to a blood vessel microcirculation chip and system for in-vitro three-dimensional tissue. The blood vessel microcirculation chip of the in-vitro three-dimensional tissue comprises a chip shell component with a culture tank, an in-vitro three-dimensional tissue is arranged in the culture tank, and the in-vitro three-dimensional tissue comprises a matrix, cells in the matrix and a microcirculation vessel network in the matrix; the microcirculation vessel network in the matrix comprises a bionic artery, a bionic vein and a self-development capillary network in the matrix. The in-vitro three-dimensional tissue blood vessel microcirculation chip and system provided by the invention have bionic artery, bionic vein and capillary networks similar to organisms, and simulate the blood vessel microcirculation process of arterial high-pressure exudation mass transfer and oxygen transfer and vein low-pressure reflux metabolism.

The invention relates to the technical field of photoelectric tweezers, in particular to a system for achieving operation of the photoelectric tweezers. According to the technical scheme, the system comprises a camera, an imaging barrel lens, a beam combiner, a projection system, a fluorescence module, an objective lens turntable, an electric XYZ platform, a signal generation system and a control module, the camera captures a microscopic objective lens imaging light beam through the imaging barrel lens, and the beam combiner is used for projecting a microscopic light beam and reflecting a projection light beam; the projection system is composed of an LED light source, an array reflector set and the like and projects controllable light spots under an objective lens. The fluorescent module switches a bright field mode and a fluorescent mode; the objective lens turntable automatically switches multiplying power; the electric XYZ platform is used for fixing and positioning a biological chip; the signal generation system outputs an electric signal of the control chip; the control module is divided into a camera control part, a projection part and a motor part to cooperatively complete photoelectric tweezer operation. The photoelectric tweezers operation and the fluorescence operation are completed by one system, and the photoelectric tweezers operation and the fluorescence operation are controlled through a proper control process.

The invention relates to a biochemical substance analysis system and a biochemical substance analysis method, the biochemical substance analysis system comprises a biochemical reaction module, a liquid changing device, an imaging detection device, a transfer device and a carrying platform, the carrying platform is used for carrying a biochip, and the transfer device is used for transferring the carrying platform at different sites; the liquid changing device is used for adding liquid to the biochip; the biochemical reaction module is used for enabling an analyte to be detected in the biochip to react; and the imaging detection device is used for executing imaging detection analysis on the reacted analyte to be detected. The liquid changing device, the imaging detection device, the transferring device and the at least two groups of biochemical reaction modules are integrated in one system, so that the integrated operation of sample adding, biochemical substance analysis and imaging can be realized, the system structure is simplified, the reagent loss is reduced, the detection flux and the detection efficiency are improved, and the detection cost is reduced.

The present invention relates to a biochip (100) optimized for spectroscopy imaging comprising a reservoir and a culturing chamber (110) comprising an observational interface (115) configured to allow spectroscopy measurement of the culturing chamber (110) through the observational interface (115).

The invention relates to biomedical engineering, and discloses a biochip screening method of a therapeutic agent based on intestinal brain axis regulation and application thereof. The screening method comprises the following steps: performing intestinal wall cell culture or microglial cell culture by adopting a metasurface plasmon resonance biochip of which the surface is subjected to bionic treatment to obtain an intestinal wall cell adherent chip board or a microglial cell adherent chip board; the method comprises the following steps: adding a culture medium containing a chemotherapeutic drug or lipopolysaccharide into a cell-adherent chip board for culture I, then removing the culture medium, adding a therapeutic agent to be detected for culture II to obtain a culture solution, and detecting the cell repair effect of intestinal wall cells or microglial cells in the culture solution, and screening to obtain a therapeutic agent with a repairing effect on intestinal wall cells and / or microglial cells. The screening method can be used for efficiently and accurately screening the therapeutic agent with dual effects of intestinal regulation and neuroprotection from a plurality of therapeutic agents to be detected, and can be used for dynamically monitoring and exploring the interaction condition of the therapeutic agent and cells on line in an unmarked and real-time manner.

Embodiments of the present invention provide a sensing apparatus. The sensing apparatus includes a biochip unit and a receptacle device. The biochip unit includes a reservoir configured to receive a predetermined amount of an analyte, a plurality of working electrodes configured to be in contact with the analyte, a reference electrode and a counter electrode configured to complete an electrical circuit with the plurality of working electrodes. The receptacle device includes a receptacle unit configured to be electrically and mechanically coupled to the biochip unit, a function generator electrically coupled to the receptacle unit, a memory unit configured to store machine-readable instructions, and a processor operably connected to the memory unit. The processor is enabled by the machine-readable instructions to determine a plurality of deduced values corresponding to a plurality of analyte parameters of the analyte from the plurality of observed values of the plurality of predetermined parameters.

The application provides a preparation method of a dot array biochip. The application forms a nano-pore dot array on a chip substrate through nano-imprinting, uniformly forms a functional modification layer on the dot array surface through a hydrogel, and then performs etching treatment on the substrate by using a deflected ion beam; an angle between an ion beam incident direction and a surface of a slide stage is θ during etching, so as to perform selective etching on the functional modification layer; through the etching with the angle, the functional modification layer of the bottom and part of the sidewall of the nano-pore can be reserved, and the functional modification layer of other parts is etched clean by the ion beam; the ion source of the IBE is argon, which is stable in chemical property and has no obvious influence on the chemical composition of the material, so that the damage of chemical residues to the performance of the functional material is avoided; the IBE does not produce chemical reaction byproducts, so that the risk of surface residual particles and pollution is reduced; the ion beam energy of the IBE is adjustable, the relatively mild etching avoids damage to the substrate, and the influence on the lattice and surface state of the surface layer material is small.

The invention relates to a detection method, in particular to a method for rapidly detecting alpha and beta-lactoglobulin in auxiliary material lactose based on a microarray chip technology. On the basis of an indirect competitive enzyme-linked immunosorbent assay principle and a silver enhanced developing technology, a high-sensitivity microarray chip detection platform is successfully constructed by virtue of a biological chip scanner, and rapid quantitative analysis of alpha-lactalbumin and beta-lactoglobulin in the auxiliary material lactose for injection is realized. According to the method, an antigen-antibody specific recognition mechanism and a nano-silver signal amplification mechanism are integrated, and the detection efficiency and sensitivity are remarkably improved through the high-throughput layout of the microarray chip. In the aspect of methodology performance, the technology has the remarkable advantages of simple and convenient operation process, short detection time, high sample treatment flux, excellent specificity (CRlt; 1%) and the like.

An automatic sample adding and detecting device for a biological chip relates to the technical field of biological chip detection and comprises a platform, a coordinate positioning mechanism, a supporting plate, a biological chip, a sealing plate, a detection hole, an automatic buffer solution feeder and a biological sample feeder, and the automatic buffer solution feeder and the biological sample feeder are identical in structure. Each feeder comprises a feeder body and a driving mechanism used for controlling the feeder body to quantitatively discharge liquid, a support is further fixedly arranged at the top end of the platform, an optical fiber probe is arranged on the support, and a shooting camera is arranged on the support on one side of the optical fiber probe. The liquid adding device does not need frequent sampling, can ensure that the liquid adding amount each time is consistent, avoids the tedious structure and operation of repeated sampling, promotes the detection result to be more accurate due to the uniform liquid adding amount, and is simple in structure, convenient to use, low in manufacturing cost, convenient to maintain and suitable for popularization and use.