252 results about "Biochip" patented technology

The invention discloses a liquid drop routing method under fluid constraint in a digital micro-fluidic biochip based on reinforcement learning and imitation learning, and aims to solve the technical problems of low planning efficiency, low success rate and weak generalization ability of a traditional liquid drop routing algorithm under fluid constraint. The method adopts a strategy combining imitation learning and reinforcement learning and comprises the following steps: firstly, generating expert data by utilizing an expert algorithm meeting fluid constraint after transformation, and pre-training a network model through imitation learning to enable the network model to quickly grasp a basic routing strategy; the model and environment interaction are autonomously optimized through reinforcement learning, and the adaptability to the dynamic environment is improved. And finally fusing the weights to form a final model. Through the hybrid training framework, the success rate and planning efficiency of droplet routing are remarkably improved, and the generalization ability of the model to chip environments with different sizes is enhanced.

This invention belongs to the field of biochip technology, specifically an erasable single nucleotide polymorphism (SNP) genotyping chip and its detection method. The erasable SNP genotyping chip provided by this invention is prepared using polystyrene microspheres, capture probes, and a silicon substrate. In the SNP recognition stage, it relies on a FEN1 enzyme-mediated invasive reaction. Based on the technical characteristics of this invasive reaction, downstream probes A and B are designed with self-protective hairpin structures, preventing false positive signals from hybridization with the capture probe even when added in excess, thus significantly improving detection specificity. The detection method of the erasable SNP genotyping chip provided by this invention overcomes the long-standing problem of high application costs caused by the single-use nature of solid-phase SNP gene detection chips.

A biochip for use in detecting biological materials is provided. The semiconductor device includes a substrate (110), semiconductor layers (120, 121, 122, 123), an insulating layer (130), IL1, IL2, metal layers (140, 141, 142, 143), a solution (200, 210, 220), a first protective layer (150, 152, 153) and a second protective layer (160, 161, 162, 163). The semiconductor layer is disposed on the substrate and has a reaction region. The insulating layer is disposed on the semiconductor layer and has a first opening (O1) exposing the reaction region. The metal layer is disposed on the insulating layer (130). The metal layer includes a source electrode (141), a drain electrode (142) and a surrounding wall structure (143). The source electrode and the drain electrode are electrically connected to the semiconductor layer, respectively. The surrounding wall structure has a first opening (O1). The opening surrounds the source electrode and the drain electrode. A first protective layer is disposed on the metal layer and has a second opening O2. A second protective layer is disposed on the first protective layer and has a third opening O3 and a fourth opening O4. The second protective layer includes a first protrusion 161, a second protrusion 162 and a third protrusion 163. The first protrusion is disposed corresponding to the source electrode, the second protrusion is disposed corresponding to the drain electrode, and the third protrusion is disposed corresponding to the surrounding wall structure, surrounding and defining the fourth opening. A solution containing bioprobes 210 is disposed in the opening. In the normal direction of the substrate, the third opening overlaps the second opening and the first opening, and the fourth opening exposes the first protective layer.

The invention provides an FPVA biochip layout method considering reagent type difference in unit multiplexing, which comprises the following steps of: modeling a biochip layout task as a Markov decision process: according to the size of an input FPVA chip, the dependency relationship of each operation in a biochemical reaction sequence diagram, the reagent type contained in each operation and a binding scheduling scheme, determining the FPVA biochip layout task; initializing an FPVA mesh model and defining a state space, an action space and a reward function; wherein the reward function is set according to the total unit multiplexing complexity of the current layout scheme, the legality of the current component layout and the distance between the component and the center point of the FPVA chip; and finally, completing the FPVA biochip layout through the dual deep Q network based on a Markov decision process. By designing a reward function, the unit multiplexing complexity is minimized, and the distance between components containing the same type of reagents is reduced, so that the purposes of minimizing the cross contamination degree of a final layout scheme, the total length of a fluid transportation path and the bioassay completion time are achieved.

The invention relates to the technical field of veterinary epidemic disease detection, and discloses a veterinary epidemic disease remote monitoring and intelligent diagnosis system, which comprises a multi-mode biosensor array including an implantable miniature biochip, a wearable flexible physiological monitoring patch and an environment sensing sensor cluster, the implantable miniature biochip is used for being implanted into a key tissue organ area in an animal body and collecting microscopic physiological data such as cellular metabolism signals and local immune reaction marker concentration in real time, and the wearable flexible physiological monitoring patch is used for being attached to the body surface of the animal. By means of the multi-mode biosensor array and the edge intelligent preprocessing unit, animal physiology and environment data are accurately monitored in real time. The multi-modal biosensor array covers the implantable miniature biochip, the wearable flexible physiological monitoring patch and the environment sensing sensor cluster, and can comprehensively collect in-vivo microscopic physiological data, body surface macroscopic physiological parameters and feeding environment information of animals.

The invention discloses a reduced graphene oxide biochip based on a piezoelectric substrate and a preparation method and application thereof, the reduced graphene oxide biochip based on the piezoelectric substrate comprises a sound transduction unit and at least one detection unit connected to the sound transduction unit, and a microfluid channel layer is arranged between the sound transduction unit and the detection unit. A reduced graphene oxide layer is directly formed on the surface of a piezoelectric layer of a detection unit, a biological recognition element is fixed on the reduced graphene oxide layer, and the biological recognition element is used for being combined with target biological molecules to generate a reaction product with electron donating and receiving capacity. On the basis, the acoustic transduction electrode design of a concentric interdigital electrode structure is adopted, and the acoustic surface wave intensity which is more concentrated compared with other electrode structures is generated by utilizing the focusing effect of the concentric electrode structure; the flow velocity of the fluid to be measured in the microfluid channel is faster, and the directivity is stronger and more concentrated.

An integrated slide, a liquid changing device, a biochemical substance analysis system and an analysis method.The integrated slide comprises a biochip and an ink jet chip packaged above the biochip, the biochip and the ink jet chip define a biochemical reaction cavity, the ink jet chip is communicated with the biochemical reaction cavity, and the biochemical reaction cavity is communicated with the liquid changing device. Reagents required by biochemical reaction can be added into the biochemical reaction cavity, or reacted residual reagents in the biochemical reaction cavity can be discharged; and the biochip can realize in-situ signal detection. According to the biochemical substance analysis system applying the integrated slide glass, integrated operation of sample adding, biochemical substance analysis and imaging can be realized, the system structure is simplified, the reagent loss is reduced, the detection efficiency is favorably improved, and the detection cost is reduced.

The present invention relates to a biochip (100) optimized for spectroscopy imaging comprising a reservoir and a culturing chamber (110) comprising an observational interface (115) configured to allow spectroscopy measurement of the culturing chamber (110) through the observational interface (115).

The invention relates to biomedical engineering, and discloses a biochip screening method of a therapeutic agent based on intestinal brain axis regulation and application thereof. The screening method comprises the following steps: performing intestinal wall cell culture or microglial cell culture by adopting a metasurface plasmon resonance biochip of which the surface is subjected to bionic treatment to obtain an intestinal wall cell adherent chip board or a microglial cell adherent chip board; the method comprises the following steps: adding a culture medium containing a chemotherapeutic drug or lipopolysaccharide into a cell-adherent chip board for culture I, then removing the culture medium, adding a therapeutic agent to be detected for culture II to obtain a culture solution, and detecting the cell repair effect of intestinal wall cells or microglial cells in the culture solution, and screening to obtain a therapeutic agent with a repairing effect on intestinal wall cells and / or microglial cells. The screening method can be used for efficiently and accurately screening the therapeutic agent with dual effects of intestinal regulation and neuroprotection from a plurality of therapeutic agents to be detected, and can be used for dynamically monitoring and exploring the interaction condition of the therapeutic agent and cells on line in an unmarked and real-time manner.

Embodiments of the present invention provide a sensing apparatus. The sensing apparatus includes a biochip unit and a receptacle device. The biochip unit includes a reservoir configured to receive a predetermined amount of an analyte, a plurality of working electrodes configured to be in contact with the analyte, a reference electrode and a counter electrode configured to complete an electrical circuit with the plurality of working electrodes. The receptacle device includes a receptacle unit configured to be electrically and mechanically coupled to the biochip unit, a function generator electrically coupled to the receptacle unit, a memory unit configured to store machine-readable instructions, and a processor operably connected to the memory unit. The processor is enabled by the machine-readable instructions to determine a plurality of deduced values corresponding to a plurality of analyte parameters of the analyte from the plurality of observed values of the plurality of predetermined parameters.

The application provides a preparation method of a dot array biochip. The application forms a nano-pore dot array on a chip substrate through nano-imprinting, uniformly forms a functional modification layer on the dot array surface through a hydrogel, and then performs etching treatment on the substrate by using a deflected ion beam; an angle between an ion beam incident direction and a surface of a slide stage is θ during etching, so as to perform selective etching on the functional modification layer; through the etching with the angle, the functional modification layer of the bottom and part of the sidewall of the nano-pore can be reserved, and the functional modification layer of other parts is etched clean by the ion beam; the ion source of the IBE is argon, which is stable in chemical property and has no obvious influence on the chemical composition of the material, so that the damage of chemical residues to the performance of the functional material is avoided; the IBE does not produce chemical reaction byproducts, so that the risk of surface residual particles and pollution is reduced; the ion beam energy of the IBE is adjustable, the relatively mild etching avoids damage to the substrate, and the influence on the lattice and surface state of the surface layer material is small.

The invention relates to a detection method, in particular to a method for rapidly detecting alpha and beta-lactoglobulin in auxiliary material lactose based on a microarray chip technology. On the basis of an indirect competitive enzyme-linked immunosorbent assay principle and a silver enhanced developing technology, a high-sensitivity microarray chip detection platform is successfully constructed by virtue of a biological chip scanner, and rapid quantitative analysis of alpha-lactalbumin and beta-lactoglobulin in the auxiliary material lactose for injection is realized. According to the method, an antigen-antibody specific recognition mechanism and a nano-silver signal amplification mechanism are integrated, and the detection efficiency and sensitivity are remarkably improved through the high-throughput layout of the microarray chip. In the aspect of methodology performance, the technology has the remarkable advantages of simple and convenient operation process, short detection time, high sample treatment flux, excellent specificity (CRlt; 1%) and the like.

An automatic sample adding and detecting device for a biological chip relates to the technical field of biological chip detection and comprises a platform, a coordinate positioning mechanism, a supporting plate, a biological chip, a sealing plate, a detection hole, an automatic buffer solution feeder and a biological sample feeder, and the automatic buffer solution feeder and the biological sample feeder are identical in structure. Each feeder comprises a feeder body and a driving mechanism used for controlling the feeder body to quantitatively discharge liquid, a support is further fixedly arranged at the top end of the platform, an optical fiber probe is arranged on the support, and a shooting camera is arranged on the support on one side of the optical fiber probe. The liquid adding device does not need frequent sampling, can ensure that the liquid adding amount each time is consistent, avoids the tedious structure and operation of repeated sampling, promotes the detection result to be more accurate due to the uniform liquid adding amount, and is simple in structure, convenient to use, low in manufacturing cost, convenient to maintain and suitable for popularization and use.

The application discloses a kind of solid sample automatic sample adding biochip, detection method and detection device, biochip includes biochip substrate and detection area, further include container and cavity, the container is fragile material, the cavity is the hollow cavity with top opening, the lower portion of the cavity is communicated with sample introduction microtube line, another end of the sample introduction microtube line is communicated with the detection area, the side wall of the cavity is provided with breakage opening, the bottle mouth of the container is provided with plunger with interference fit, the maximum outer diameter of the plunger is greater than the maximum outer diameter of the container, the plunger is interference fit with the inner wall of the cavity, the container and the breakage opening are located at the same horizontal position.By the design of the application, after the container is broken, the container fragments and freeze-dried powder will all fall to the bottom of the cavity under the weight, automatic addition is realized.Freeze-dried reagent storage requires high, after being stored separately with container, the air-tightness requirement of transfer cavity and substrate is reduced.

The utility model relates to the technical field of biochip manufacturing equipment, in particular to a membrane uncovering mechanism for a membrane, which comprises a working table, a sliding frame and a clamping jaw assembly, the working table is provided with a waste material port, the sliding frame is horizontally and slidably mounted on the working table through a sliding component, and the clamping jaw assembly is arranged on the working table. The clamping jaw assembly is installed on the sliding frame in a lifting mode through a lifting assembly, a clamping jaw used for uncovering a film is arranged below the clamping jaw assembly, and after the film is uncovered, the clamping jaw horizontally slides and moves to the waste material opening through the sliding frame to discard the PDMS layer. According to the film uncovering mechanism, through cooperation of the sliding assembly, the lifting assembly, the clamping jaw assembly and the like, automatic operation of the film uncovering process can be achieved, the film uncovering efficiency is improved, and damage to the substrate is avoided.

A method for analyzing a biochip image is provided, including: (S1) acquiring and preprocessing the biochip image to obtain a preprocessed image; (S2) performing a correction for angle deflection on the preprocessed image to obtain a deflection-corrected image; and (S3) performing an enhancement processing on the deflection-corrected image, and identifying a positive or negative of an area of interest in the preprocessed image according to an image on which the enhancement processing has been performed. An apparatus (100) for analyzing a biochip image, a method for analyzing an image, a computer device (200) and a storage medium are disclosed.

The invention belongs to the technical field of computer vision and image processing, and particularly discloses an N * N dot matrix circular target detection and deletion completion method and system based on deep learning. Comprising the following steps: preprocessing an input image and performing initial circle detection by using a pre-trained YOLO model; the detection result is filtered based on geometric constraint conditions, and misrecognition points are removed; performing row and column clustering and structure grouping on the normal points; performing preliminary completion on partial missing points according to known dot matrix information; extrapolation completion is carried out on the missing condition of the whole row or the whole column; and finally outputting coordinates and radiuses of all circle centers. According to the method, deep learning and geometric rule constraints are fused, the problems of noise interference, bubble interference and dot matrix missing under a complex image background can be effectively solved, the accuracy, integrity and robustness of circular dot matrix detection are remarkably improved, and the method is suitable for dot matrix automatic identification and analysis in multiple detection scenes such as biochip and microfluidic detection.

A sample loading device and a gene sequencer, the sample loading device comprising: a fixed seat assembly, a gland assembly and a cap assembly, the fixed seat assembly having a fixed position for placing a slide frame loaded with a biochip; the gland assembly is rotatably connected to the fixed seat assembly, and the gland assembly is further snap-connected with the fixed seat assembly to fix the slide frame in the fixed position, and the gland assembly is provided with a positioning hole for installing a pipette head; the cap assembly is detachably arranged on the gland assembly and covers the positioning hole, and the cap assembly is used for pressing the pipette head in the positioning hole to provide a pushing force for the liquid in the pipette head. The sample loading device of the present application can realize the fixation of the slide frame by the gland assembly and the fixed seat assembly, and the cap assembly arranged on the gland assembly can provide a pushing force for the liquid in the pipette head, promote the flow of the internal liquid, and be beneficial to the automatic liquid passing of the biochip.

The invention relates to a synchronous detection method and system for small molecules and protein, and the synchronous detection method comprises the following steps: respectively preparing a small molecule detection antibody and a protein detection antibody marked by carboxyl fluorescent microspheres, and mixing the small molecule detection antibody and the protein detection antibody to obtain a mixed detection antibody; respectively preparing a microsphere-labeled capture micromolecule antigen and a protein antibody; preparing a microsphere array biochip with two detection areas; carrying out sample application on the microsphere-labeled captured micromolecule antigen and the protein antibody to the two detection areas; mixing a sample to be detected with the mixed detection antibody according to different proportions; slowly adding the sample mixed solution into two detection areas of the microsphere array biochip respectively; scanning and imaging the two detection areas of the microsphere array biochip respectively, identifying all fluorescent spot information in a fluorescent image, and obtaining the concentration of small molecules and proteins in a sample to be detected through a Poisson distribution principle or by establishing a standard curve. According to the invention, synchronous detection of small molecules and proteins can be realized.

The invention relates to the technical field of biological chips, and provides a multi-array micro-fluidic chip and a preparation method thereof.The multi-array micro-fluidic chip comprises a first chip body, a second chip body and a porous membrane; at least two first channels distributed at intervals are constructed in the first chip body, every two adjacent first channels are arranged in parallel, inlets of every two first channels converge to a first public inlet, and outlets of every two first channels converge to a first public outlet. At least two second channels distributed at intervals are constructed in the second chip main body, the positions of the second channels correspond to those of the first channels one to one, inlets of every two second channels converge to a second public inlet, and outlets of every two second channels converge to a second public outlet; and the porous membrane is clamped between the first chip main body and the second chip main body. Through fluid inlet and outlet sharing and symmetrical flow division design, the consistency of experiment conditions can be guaranteed, and the structural cost and the operation complexity can be reduced.

The application provides a disc-based multi-staining biochip detection method based on nanoparticle disturbance laser signal, which comprises the following steps: S1, determining staining substances of various to-be-detected organisms according to the concentration detection range of various to-be-detected organism solutions; S2, preparing a staining organism array of various to-be-detected organism solutions on a disc base to obtain a disc-based staining biochip; S3, scanning the disc-based staining biochip based on a blue light drive; and S4, analyzing the BIS error number at each logical position in the scanning result, and determining the concentration of the to-be-detected organism solution according to the BIS error number size. The application can realize the simultaneous detection of the concentrations of various organism solutions, and improves the detection efficiency.

The present application relates to the technical field of biochip, in particular to a multi-waveband synergic release permeation-quantitative biochip, which comprises a substrate, a permeation function layer and a gold plating layer stacked from bottom to top; the substrate is made of graphene material with a thickness of 0.05-0.1mm, used for receiving and storing environmental energy; the permeation function layer has a thickness of 0.1-0.3mm, which is made of a mixture of nano far infrared powder, anion powder, magnetic powder and nano catalytic material with a mass ratio of 3:2:2:1, and then compounded with a biocompatible adhesive; the present application simultaneously releases magnetic wave, far infrared wave, anion wave and quantitative wave, and has the dual functions of human physiotherapy and material purification, solves the problem of single function of the existing products, the energy storage capacity of the graphene substrate and the energy aggregation effect of the gold plating layer greatly improve the energy utilization efficiency, prolong the chip action time, the permeation technology strengthens the waveband penetration capacity, and when acting on the human body, it does not need to be pasted on the skin, and when acting on the material, it does not need to be soaked, and the use scene is flexible.

The invention relates to a gene sequencer, in particular to fluid supply of the gene sequencer, and provides the gene sequencer, a refrigeration device and a sequencing method of the gene sequencer. The gene sequencer comprises: a fluid system for conveying a sample to be detected and / or a reagent for detection to a biochip; the storage module is used for placing a liquid container for containing a to-be-detected sample and / or a detection reagent; and a storage module lifting device. When the liquid suction module sucks liquid through the reagent needle, the height of the storage module can be increased, and compared with the mode that the reagent needle is inserted into a to-be-detected sample and / or a detection reagent in a liquid container by reducing the height of the liquid suction module, liquid in the liquid conveying pipe can flow into the biological chip more conveniently, release of dissolved gas in the liquid is reduced, and the detection efficiency is improved. Therefore, the technical problem that bubbles easily appear in the infusion tube of the gene sequencer can be solved.

The application provides a continuous microfluidic biochip underflow layer physical collaborative design method, which realizes the underflow layer physical design work in three stages.(1) Logic design stage: through the first proposed logic layout and component direction layout adjustment method, the excellent logic position and logic direction of the component are obtained, which greatly improves the efficiency while ensuring the high-quality layout solution.(2) Component mapping and bounding box gap layout adjustment stage: based on the bounding box strategy, the component logic design result of the previous stage is mapped to the actual physical design space, and the best bounding box gap is obtained through the bounding box gap layout adjustment.(3) Shrink layout adjustment stage: based on the connection graph relationship between components, two new layout adjustment methods, namely along-flow channel shrinkage and multi-graph shrinkage, are proposed, which further efficiently improves the quality of the underflow layer physical design. The application not only effectively improves the quality of the underflow layer physical design, but also greatly improves the efficiency.

A biochip device comprising a substrate constituted by at least one plate of material forming a multimode planar waveguide and carrying chromophore elements suitable for emitting fluorescence in response to excitation by guided waves having an evanescent portion, the device being characterized in that it includes coupling means for coupling excitation light with the waveguide in the form of guided waves, the coupling means being substantially non-directional.

The present invention relates to the technical field of biochips and provides a DNA origami microarray biochip based on plasmon surface lattice resonance, comprising a chip substrate and arrayed DNA origami-plasmonic nanostructure-biomolecular probe units. A preparation method thereof comprises the following steps: extending a class A single strand from the DNA origami surface for grabbing and connecting plasmonic nanoparticles and biomolecular probes; then modifying the plasmonic nanoparticles and biomolecular probes with a class B single strand DNA that base-pairs complementary with the class A single strand; constructing hydrophilic array-arranged points on the substrate surface; connecting DNA origami to each point to form a microarray; and assembling biomolecular probes and plasmonic nanoparticles on the DNA origami through base-pairing complementary to form a plasmon-biomolecular probe microarray. The biochip can be customized for the detection of multiple or single pathogens, and the manufacturing method is economical and efficient, meeting personalized medical needs.

The application discloses a kind of based on nano-silver-porous silicon simultaneously SERS detection variety of mycotoxin biochip sensor and its preparation method and application, the sensor includes SERS substrate and SERS label two parts compositions, SERS substrate is by porous silicon surface magnetron sputtering on silver nanoparticles and silver nanoparticles surface connection three kinds of mycotoxin artificial antigen composition, SERS label is connected with nile blue and corresponding mycotoxin antibody gold nanoparticles.The application can be combined by each mycotoxin and porous silicon silver surface mycotoxin artificial antigen mycotoxin antibody on SERS label, by detecting the Raman signal of NBA on SERS label surface, realize three kinds of mycotoxin simultaneous quantitative analysis, detection speed is fast, sensitivity is high, while various toxins in gualou, malt and kudzu root recovery rate is all greater than 75%, the coefficient of variation of same batch is less than 5.2%, and the coefficient of variation of different batches is less than 7.8%.

The application provides a continuous micro-fluidic biochip high-level synthesis method considering volume management, is based on a discrete particle swarm algorithm with high solving efficiency, and comprises the following key technologies: 1) a particle coding scheme suitable for the high-level synthesis problem considering volume management is provided; 2) an automatic scheduling scheme generation method is provided, which can accurately generate a corresponding operation scheduling scheme according to the particle coding; and 3) according to the characteristics of the problem, a corresponding particle updating strategy is designed, and the convergence of the algorithm is accelerated. The application can obtain a high-level synthesis scheme satisfying volume constraints and having high quality in a shorter time.

The application discloses a small-molecule multi-connection detection kit based on a biochip, a preparation method and a detection method thereof. The kit comprises nano-gold particle labeled antibodies, a multi-connection detection chip plate and a detection buffer. The nano-gold particle labeled antibodies comprise nano-gold particle labeled florfenicol antibodies and enrofloxacin antibodies. The multi-connection detection chip plate is coated with florfenicol antigens and enrofloxacin antigens in detection holes. The detection buffer is composed of 10-40 mM phosphate buffer and 0.05-0.5 wt% surfactant S17. The application can simultaneously detect florfenicol and enrofloxacin small molecules by using one detection buffer, thereby saving time and labor.

The utility model discloses an autoimmune liver disease antibody detection chip, which belongs to the technical field of biochips and diagnostic reagents and comprises a reaction membrane, an upper shell and a lower shell, the upper shell and the lower shell can be buckled together, a water absorption layer is arranged between the upper shell and the lower shell, a blood filtering component is movably arranged above the upper shell, and a water outlet is formed in the lower shell. The blood filtering assembly comprises a blood filtering cup and a blood filtering film, and the blood filtering film is fixedly connected with the blood filtering cup. According to the autoimmune liver disease antibody detection chip disclosed by the utility model, the blood filtering film can be flexibly mounted and dismounted, and the waste of the blood filtering film is avoided.