Universal biochip as well as preparation method and application thereof

By forming a silica film on the substrate surface of the biochip and modifying the active groups, the DNA probe or protein is fixed, the problem of prone to rupture of the glass substrate is solved, the preparation of a general-purpose biochip is realized, and its scope of application is expanded.

CN119869636AInactive Publication Date: 2025-04-25TIANJIN ZHONGHE GENE TECH CO LTD +1
6 Cites 1 Cited by

Patent Information

Application Number
CN202510360560.6
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2025-03-26
Publication Date
2025-04-25
Estimated Expiration
Not applicable · inactive patent

AI Technical Summary

Technical Problem

Existing biochips mainly use glass slides, which have problems such as easy rupture, difficult injection molding and processing, which limits the wider application of biochips.

Method used

A universal biochip preparation method is adopted to form a silica film by soaking the substrate in a solution composed of silane reagent A, alkaline reagent and buffered saline solution, and modifying the active groups on its surface, and finally coupling with DNA probes or proteins to fix it on the surface of the substrate.

Benefits of technology

This method is suitable for various metal and non-metallic substrates. The preparation process is simple and the conditions are mild. It can effectively solve the problem of easy cracking of glass substrates and expand the scope of application of biochips.

✦ Generated by Eureka AI based on patent content.
Patent Text Reader

Abstract

The invention discloses a universal biochip as well as a preparation method and application thereof. The preparation method of the universal biochip comprises the following steps: S1, soaking a substrate in a solution consisting of a silane reagent A, an alkaline reagent and a buffer salt solution for reaction, and curing to form a silicon dioxide film on the surface of the substrate; the material of the substrate is selected from any one of glass, metal, plastic, resin and a film material; s2, soaking the substrate treated in the step S1 in a solution of a silane reagent B connected with an active group for reaction so as to modify the active group on the surface of the silicon dioxide film; and S3, carrying out a coupling reaction on the surface active group of the substrate treated in the step S2 and a DNA probe or a group in protein to obtain the universal biochip. The method is suitable for various metal and non-metal material substrates, the preparation process is simple, the condition is mild, the biological chip can be processed into the required shape according to the material, and the application range of the biological chip is further expanded.
Need to check novelty before this filing date? Find Prior Art