55 results about "Cell culture" patented technology

1. Name of the design product: Controller for controlling small cell culture tank. 2. Use of the design product: for controlling oxygen flow, stirring speed, acid and base addition ratio (i.e. pH value) and temperature of small cell culture tank. 3. Design points of the design product: in shape. 4. Picture or photo best indicating the design points: front view.

The present invention generally pertains to methods of cultivating cells and producing a recombinant protein of interest, such as dupilumab. In particular, the present invention pertains to the use of a fixed seed schedule to determine the expansion time of seed train unit operations for improved cell growth and protein titer. The protein produced by the fixed seed schedule, such as dupilumab, can be later subjected to lipase activity reduction methods.

Dual vector systems and these comprehensive mammalian host cells are provided for the expression of multi-chain molecules, with each vector employing a different promoter controlling the expression of a selectable marker. The mammalian host cells can be used to generate a recombinant protein of interest in a mammalian cell culture.

The application discloses a kind of based on HIV envelope protein chimeric exosome and its preparation method and application, belong to biological medicine technical field.The application first constructs the cell line of stable expression HIV envelope protein Env, obtains engineered exosome from cell culture supernatant separation and purification;The exosome is used as immunogen combined with adjuvant immunization experimental animal, and high-efficiency induction specific humoral immune response;Again, obtain Env antigen specificity single B cell by flow cytometry sorting, obtain antibody variable region gene by single cell lysis, reverse transcription and nest PCR amplification, cloning to expression vector and expressing in mammalian cell, finally, HIV specific neutralizing antibody is screened by binding activity, affinity and neutralizing activity;The application is combined with single B cell antibody screening technology by embedding HIV Env antigen in the form of membrane combination in exosome surface, and realizes the synergistic optimization of antigen delivery and antibody screening process.

This invention discloses a comprehensive toxicity evaluation and source tracing method for wastewater based on multi-indicator responses of algal cells. The method includes: adding the wastewater to be tested into an algal cell culture system for exposure and obtaining algal cell viability index values ​​at multiple exposure times; determining the pollution toxicity type of the wastewater based on a machine learning model and / or a multi-algal species combination detection method, and tracing the pollution source based on the pollution toxicity type; and evaluating the toxicity and risk of the wastewater based on the pollution toxicity type and algal cell viability index values ​​using a comprehensive toxicity evaluation system. This invention can identify toxicity types based on algal cell viability indicators and construct a comprehensive toxicity assessment system to comprehensively evaluate the toxicity and risk of wastewater, solving three major problems of existing technologies: static endpoint determination, neglect of species sensitivity differences, and missed detection of chronic toxicity.

To provide a multilayer film that has high oxygen permeability and can be heat-sealed to form a container that is resistant to tearing from impacts such as dropping when it contains liquid. [Solution] A heat seal layer comprising copolymer A, which is a copolymer of 4-methyl-1-pentene and propylene, further comprising copolymer B, which is a copolymer of ethylene and an α-olefin having 3 to 20 carbon atoms, or copolymer C, which is a copolymer of propylene and an α-olefin (excluding propylene) having 2 to 20 carbon atoms; and a core layer comprising (co)polymer D, which is a 4-methyl-1-pentene (co)polymer, wherein the film impact strength measured from the opposite side of the heat seal layer in accordance with ASTM-D 3420:2021 is 7 kJ / m or more, and the oxygen permeability at 23°C is 3.0 L / (m 2 ·24h·atm) or more 100.0L / (m 2 Multilayer film with a temperature of 24 hours (atm) or less.

This invention relates to the field of stem cell culture technology, and particularly to a stem cell culture device and its method of use, comprising a housing, a base plate, and a cover plate. An annular observation window is provided on the cover plate, and a detection mechanism for observing stem cells is also provided on the cover plate. By positioning the movement trajectory of the sample carrier, liquid level fluctuations caused by tilting of the culture flask during ascent are effectively avoided. Combined with the pressure regulation function controlled by the micro-flow one-way valve of the telescopic damping airbag, stable and uniform lifting and lowering of the sample carrier component is achieved. With the assistance of annular scanning by an electron microscope, multiple batches of samples can be tested without manual intervention. This effectively avoids the acceleration impact caused by traditional sample transfer methods, ensuring the stability of the stem cell suspension and effectively preventing the impact of mechanical vibration on stem cell growth, thereby effectively improving the accuracy and repeatability of stem cell detection data.

The application discloses a kind of biological cell culture with shaking table device, belong to the technical field of shaking table, including equipment base, shaking plate, drive structure, clamp and image acquisition module, image acquisition module includes the camera source that can be moved to any one clamp directly above by power drive;Clamp includes: substrate is fixedly installed on shaking plate;Stage moves up and down relative to the substrate;Several guide grooves are provided on the stage;Limiting piece is at least configured with three groups and is distributed around the center of the stage, each limiting piece moves between first position and second position relative to the substrate;Several limiting pieces are at least partially located in several guide grooves respectively;When stage moves downward, each limiting piece moves relative to corresponding guide groove, limiting piece moves from first position to second position, and the inner circle space formed by several limiting pieces around gradually reduces, to adapt to different specifications of culture dish in this way.

An apparatus for cell cultivation includes first reservoir structure, second reservoir structure and a membrane. The first reservoir structure has first matrix of pitted reservoirs and the second reservoir structure has second matrix of pitted reservoirs. The first reservoir structure, second reservoir structure and the membrane are arranged as a stack. The membrane is arranged between first reservoir structure and second reservoir structure, and the first matrix of pitted reservoirs is aligned with the second matrix of pitted reservoirs to couple pitted reservoirs of the first matrix of pitted reservoirs together with pitted reservoirs of the second matrix of pitted reservoirs via the membrane.

The present invention relates to transcriptional regulator polypeptides, polynucleotides encoding the transcriptional regulator polypeptides, and to nucleic acid constructs, vectors and host cells comprising the polynucleotides as well as methods of producing a polypeptide of interest in host cells overexpressing the transcriptional regulators, to methods of increasing the oxygen uptake rate and / or decreasing viscosity of the culture broth in a fungal host cell cultivation process, to methods of producing a transcriptional regulator polypeptide, to the use of a transcriptional regulator polypeptide, and to the production of fungal biomass.

The present invention belongs to the technical field of synthetic biology, metabolic engineering and cell cultivation. The present invention relates to a method for producing a sialylated compound comprising Gal-β1,3-[Neu5Ac-α2,6]-HexNAc-R, and a method for purifying said sialylated compound. The present invention further provides a cell for producing said sialylated compound and the use of said cell in cultivation and incubation. The present invention further relates to a method and a cell for producing a sialyltransferase having an alpha-2,6-sialyltransferase activity on the N-acetylhexosamine (HexNAc) residue of Gal-β1,3-HexNAc-R and / or Neu5Ac-α2,3-Gal-β1,3-HexNAc-R, wherein said sialyltransferase is involved in the production of a sialylated compound comprising Gal-β1,3-[Neu5Ac-α2,6]-HexNAc-R.

This invention discloses a method for preparing a xylitol dehydrogenase mutant, relating to the field of industrial biotechnology. The method involves obtaining an amino acid sequence for the xylitol dehydrogenase mutant by substituting serine at position 77 with cysteine, serine at position 106 with asparagine, and alanine at position 110 with asparagine, based on the amino acid sequence shown in SEQ ID NO. 1. A recombinant vector containing a nucleic acid molecule encoding the xylitol dehydrogenase mutant is constructed. The recombinant vector is introduced into host cells, the host cells are cultured and the xylitol dehydrogenase mutant is expressed, and cells containing the xylitol dehydrogenase mutant are collected or isolated from cells. This invention reduces intermediate accumulation and improves xylitol conversion rate.

Systems and methods for operating a cabinet irradiator. The methods comprise: receiving a sample holder in a receptacle of an XYZ stage platform of the cabinet irradiator (sample(s) is(are) disposed on the sample holder); controlling the XYZ stage platform to move the sample holder under a camera such that an optical axis is centered over a sample area of the sample holder; displaying a top-down image of the at least one sample in a first graphical user interface along with at least one virtual box having an adjustable size and being movable relative to the top-down image; receiving a user input specifying at least one area of the at least one sample that is to be irradiated via movement and or resizing of the at least one virtual box; receiving a user input selecting or defining an irradiation treatment plan; and irradiating the sample(s) in accordance with irradiation treatment plan.

This method for estimating the therapeutic effect of stem cell transplantation comprises: a step (S11) for acquiring a plurality of cell images in which a stem cell group in culture is imaged at a plurality of predetermined time points within a cell culturing period for stem cell transplantation for regenerative medicine purposes; a step (S12) for identifying stem cell regions respectively in the plurality of cell images; a step (S13) for acquiring, on the basis of the identified stem cell regions, a plurality of types of index values associated with the stem cell group with respect to each of the plurality of cell images; and a step (S14) for estimating, before the transplantation of the stem cell group obtained by the cell culturing, the therapeutic effect of the transplantation of the stem cell group on the basis of the acquired plurality of types of index values. The plurality of types of index values acquired in step (S13) include a plurality of types of index values associated with the morphologies of the stem cells.

The application belongs to the technical field of NK cells, and discloses an NK cell culture method and application of obtained NK cells in removing senescent cells and tumor cells. The NK cell culture method comprises the following steps: S1, coating the bottom of a culture container with a coating solution; S2, resuspending peripheral blood mononuclear cells and then adding them into the coated culture container obtained in S1 for culture; the coating solution in S1 contains CD16 antibody solution and CD244 antibody solution; and the components of the first activation culture medium contain IFN-gamma and OK432. The coating solution in S1 contains CD16 antibody solution and CD244 antibody solution, and the obtained NK cells have high survival rate and high activity; OK432 in the components of the first activation culture medium in S2 can simulate bacterial infection signals, IFN-gamma can activate NK cells through different signal pathways, and OK432 can enhance the cell activity of NK cells; the obtained NK cells have high activity, long life span and high response capacity, and therefore have high efficiency in removing senescent cells and tumor cells.

A nucleic acid construct comprising a first polynucleotide encoding MGAT1 gene or a homologous gene having at least 85% sequence identity thereof, a second polynucleotide encoding MGAT2 gene or a homologous gene having at least 85% sequence identity thereof, and a third polynucleotide encoding MAN2A2 gene or a homologous gene having at least 85% sequence identity thereof. A nucleic acid construct combination comprising a first, a second, and a third nucleic acid constructs, which comprise a first polynucleotide encoding MGAT1 gene or a homologous gene having at least 85% sequence identity thereof, a second polynucleotide encoding MGAT2 gene or a homologous gene having at least 85% sequence identity thereof, and a third polynucleotide encoding MAN2A2 gene or a homologous gene having at least 85% sequence identity thereof respectively. A method of regulating the high mannose glycoform content of a recombinant protein during a mammalian cell culture process is also provided, which comprises an expression of the recombinant protein and an overexpression of three proteins that are involved in an N-glycosylation pathway in a mammalian host cell. A highly effective method for reducing the high mannose glycoform content of recombinant proteins in CHO cells.

This invention discloses an automated stem cell culture chamber with precise temperature and humidity control, comprising a chamber body, a culture tray, a diffusion frame positioned above the culture tray, an adjusting and sealing mechanism, and an oxygen absorption mechanism. The diffusion frame has a first and a second permeable membrane along the arrangement direction of the culture containers. The first permeable membrane forms an oxygen supply interface, and the second permeable membrane forms a hypoxia communication interface with the oxygen absorption box. The adjusting and sealing mechanism limits the effective exposure area of ​​the two permeable membranes respectively through a first sealing plate. The oxygen absorption box contains oxygen-absorbing material and can be moved closer to or further away from the second permeable membrane to change the volume of the hypoxia buffer chamber. A second sealing plate is provided on the ventilation side of the oxygen absorption box to adjust the ventilation opening area. Thus, without changing the position of the culture tray, by adjusting the gas exchange conditions on the oxygen supply side and the hypoxia side, an adjustable oxygen concentration gradient environment is formed above the culture tray, suitable for stem cell hypoxia induction, differentiation comparison, and drug response culture experiments.

The present invention relates to the field of producing protein conjugates. The invention relates to a novel method for producing a protein conjugate comprising a protein of interest (POI) bearing a chemical moiety and a payload comprising a complementary chemical moiety. The said method comprises the extracellular expression of the POI, contacting the POI in the cell culture supernatant with the payload, allowing the formation of a covalent bond between the POI and the payload in a cell culture supernatant, thereby creating a protein conjugate, and purifying said conjugate.

Provided is a simple and efficient method of plant genome editing that does not involve the incorporation of a gene cassette for the expression system of a site-specific DNA modification protein. The method of the present invention comprises: (i) a step for mixing plant cells or a tissue containing plant cells together with a site-specific DNA modification protein and a needle-shaped inorganic compound in a liquid medium and making a disturbance to perforate the cells with the needle-shaped inorganic compound, thereby introducing the site-specific DNA modification protein into the cells; and (ii) a step for culturing the plant cells or the tissue containing the plant cells, into which the site-specific DNA modification protein has been introduced in step (i), thereby causing a DNA mutation specific to a target site in the genome of the plant cells.

This invention discloses a β-lactoglobulin mutant, its preparation method, and its application, relating to the field of genetic engineering technology. The amino acid sequence of the β-lactoglobulin mutant is shown in SEQ ID NO.1. The preparation method is as follows: designing forward primers, reverse primers, forward mutation primers, and reverse mutation primers; using wild-type β-Lg plasmid as a template, obtaining a mutant DNA fragment using overlap extension PCR technology; ligating the mutant DNA fragment into an empty vector and transforming it into competent E. coli cells for culture and identification to obtain a recombinant plasmid; transforming the recombinant plasmid into competent E. coli cells, culturing, lysing, collecting the cell supernatant, purifying, and obtaining the β-lactoglobulin mutant. This invention, through structural modification of β-lactoglobulin, improves its binding to specific IgE antibodies, enhances the immunogenicity of β-lactoglobulin allergens, and improves the sensitivity and specificity of its allergen-specific IgE antibody detection results.

This application relates to the field of stem cell culture technology and discloses an intelligent monitoring and quality assessment system for stem cell culture environment, including: a multi-parameter sensing module for real-time acquisition of multi-dimensional parameters of the stem cell culture environment, wherein the multi-dimensional parameters include temperature (T), CO2, etc. ₂ The system collects data on concentration (C), dissolved oxygen (DO), pH (P), and culture medium flow rate (V), converting the collected data into digital signals. The predictive analysis module receives real-time data from the multi-parameter sensing module and generates output results based on a preset dynamic prediction algorithm. By predicting changes in environmental parameters in advance through the dynamic prediction algorithm, and combining this with real-time optimization through residual correction, the fluctuations in key indicators such as temperature and CO₂ concentration can be minimized. For example, reducing temperature fluctuations during embryonic stem cell culture reduces the problem of decreased cell activity caused by sudden environmental changes, allowing stem cells to grow in a more stable microenvironment.

The application discloses a kind of stem cell activity detection culture equipment for treating diabetes, it is related to stem cell culture technical field, including incubator, the incubator inside is provided with culture dish, the culture dish below is provided with alternating magnetic field generator, the inside of the culture dish is placed with stem cell and culture solution, the culture solution belongs to electrolyte solution, alternating current is generated in culture dish when the alternating magnetic field generator starts.This application is separated by the setting of partition ring and piston ring, cooperates alternating magnetic field generator, realizes the separation of normal cell and dead stem cell under the action of centrifugal force, monitoring mechanism cooperates piston ring, detects the cell in the edge of culture dish, recycles dead stem cell using inactivation space, observer can clearly understand the approximate situation of stem cell cultivation in culture dish, the setting of observation mechanism, and cooperate with protective ball and alternating magnetic field generator, reduce stem cell stacking, improve the observation effect of stem cell for researchers.

The application belongs to the field of virus culture, and provides a CHO cell, an application, and a virus culture method. In the CHO cell, a gene of bone marrow stromal cell antigen 2 is inactivated. The virus culture method comprises the following steps: (1) constructing the CHO cell in which the gene of bone marrow stromal cell antigen 2 is inactivated, and performing sequencing identification; (2) detecting the proliferation activity of the CHO cell in which the gene of bone marrow stromal cell antigen 2 is inactivated; (3) culturing the CHO cell in which the gene of bone marrow stromal cell antigen 2 is inactivated, and then infecting the virus; and (4) detecting the proliferation of virus nucleic acid and the virus titer. The CHO cell in which the gene of bone marrow stromal cell antigen 2 is knocked out is used to culture and expand the virus, the restriction of the CHO cell on virus budding is released, and the isolation efficiency of the influenza virus and the virus infection titer are improved.

This invention relates to the field of tissue engineering technology, specifically disclosing a deer placental stem cell exosome and its application in cosmetics. The deer placental stem cell exosome preparation includes the following steps: cleaning, mincing, enzymatically hydrolyzing, centrifuging, washing, and culturing sika deer placental tissue to obtain deer placental mesenchymal stem cells; culturing the deer placental mesenchymal stem cells in 3D and collecting the culture medium; centrifuging, filtering, and tangentially filtering the culture medium to obtain a purified exosome solution; adjusting the pH of the purified exosome solution and adding Mal-PGA solution for coupling modification to obtain a polyglutamic acid-exosome solution; adding a freeze-drying protectant to the polyglutamic acid-exosome solution and then drying to obtain the deer placental stem cell exosome. The prepared deer placental stem cell exosome can be used as an active ingredient in the preparation of cosmetics with moisturizing, repairing, soothing, and skin-improving effects.

The present invention is in the technical field of synthetic biology, metabolic engineering and cell cultivation. The present invention describes methods for the production of a compound comprising N-acetylglucosamine-beta-1,6-galactose-beta-1,4-Z1-Z2-R, wherein Z1 is glucose or N-acetylglucosamine (GlcNAc), wherein Z2 is absent or is a saccharide, and wherein R is absent or is selected from the list consisting of a peptide, a protein and a lipid. The invention further describes a cell that is capable to produce said compound.