168 results about "Bioprocess" patented technology

The invention provides an automatic stirring device in the field of fluid machinery and bioreactors, focusing on enhancing fluid control efficiency and consistency in biological cultivation processes. It utilizes a flexible channel body or reactor body to facilitate the flow of nutrient solution along specific channels (such as S-shaped flexible channels or S-shaped loop channels), designed to reduce shear forces and achieve iso-level uniform flow. A power system and monitoring and control system are employed to regulate the flow of nutrient solution and monitor cultivation conditions in real-time, thus supporting various bioprocess cultivation requirements.

The present invention relates to a system for loading and unloading agents in the form of particulate solid material, used for carrying out a physical, chemical, and / or biological process in one or more tanks. The system includes: an intermediate tank, a pump, and a pneumatic conveyor. The intermediate tank and the pump are fluidically connected. For unloading a tank containing saturated or degraded solid material particles, an empty or partially empty solid material tank is fluidically connected to the intermediate tank and the tank containing saturated or degraded solid material particles. For loading a tank, a solid material tank containing unsaturated or undegraded solid material particles is fluidically connected to the intermediate tank.

The invention discloses a coal bed gas biological yield increase effect dynamic monitoring and evaluation method which comprises the following steps: arranging a multi-parameter sensor in a target coal bed gas well site, and collecting background data before bacterium injection; injecting a microbial bacterial solution and a nutrient solution into the coal seam according to the experimental design, and recording parameter information in the injection process; periodically collecting a gas production sample, and analyzing gas components; analyzing microbial community structure and abundance change in the water sample; combining the gas generation rate, the isotope characteristics and the microflora analysis result to identify a gas production path and calibrate a key functional flora; and constructing a mathematical model containing microbial activity, coal quality conversion and gas production efficiency, forming an evaluation index system, and outputting field process optimization suggestions based on the model. According to the method, a scientific basis is provided for dynamic optimization of the yield increasing process, and compared with a traditional laboratory method, the method can achieve systematic analysis of the in-situ microorganism process and quantitative evaluation of the gas production effect, and has good field adaptability and popularization value.

The present invention relates to a system, method, and use for the unloading of solid material particles used in a physical, chemical, and / or biological process in a tank and for the inertization of the tank. The receiving tank and the pump are fluidically connected. The inert gas supply device is fluidically connected to the tank and to at least one injector. The at least one injector is arranged along the transport line that connects the tank to the receiving tank and is configured to inject inert gas into the transport line. The pump is configured to generate negative pressure in the receiving tank and in the tank, and the inert gas supply device is configured to insert inert gas into the tank and supply inert gas to at least one injector, to allow the displacement of solid material particles from the tank to the receiving tank.

The utility model discloses a table type glass bioreactor and relates to the technical field of bioreactors. The bioreactor comprises a bioreactor body, wherein a sealing cover is arranged at the top of the bioreactor body. The moving mechanism can drive the stirring mechanism to rotate while moving up and down, so that the stirring mechanism is in a working state of rotating while moving up and down in the bioreactor body, and high-temperature liquid positioned below the bioreactor body can flow to an upper area; meanwhile, the liquid with lower temperature above the bioreactor body flows to the area with higher temperature below the bioreactor body, so that the temperature gradient of different height positions in the bioreactor body is broken, even in the area far away from the heating plate, the uniform distribution of the temperature can be realized, and the consistency of the temperature in the bioreactor is improved; and biological processes such as cell culture and fermentation are facilitated.

The present disclosure provides methods, systems and products for controlling a bioprocess comprising a cell culture in a bioreactor, wherein the bioprocess is operated as a perfusion bioprocess the methods use a model predictive control to identify values of manipulated variables that optimise an objective function, the objective function including one or more constraints and / or one or more terms that are associated with predictions obtained from a method of simulating a chromatographic process that is coupled with the bioprocess for processing of a harvest stream of the bioprocess.

The present disclosure relates to a system and method for continuous bioprocessing simulation utilizing parameterized and pre-validated model components. The system selects validated equations from a catalogue to construct modular unit operation models, which are then instantiated into configured instances. These instances are interconnected into an integrated flowsheet simulation, which is co-simulated to capture the transient behavior of components with different time constants. The parametrized, modular modeling approach enables rapid and robust simulation of continuous biopharmaceutical production processes by assembling simulations from pre-qualified building blocks. This facilitates efficient digital prototyping and analysis of integrated continuous bioprocess systems.

To provide a method for producing 6-hydroxyhexanoic acid (6-HHA) with increased production efficiency through a bioprocess.SOLUTION: A method for producing 6-hydroxyhexanoic acid comprises culturing a bacterium belonging to the genus Shewanella in a medium containing adipic acid under metabolically controlled conditions, where the bacterium has a carboxylic acid reductase and an aldehyde reductase.SELECTED DRAWING: None

The invention discloses a bioprocess chromatography column comprising: a) a bed chamber delimited by at least one side wall, a first bed support screen and a second bed support screen; b) a first end wall, secured to or integral with the side wall(s), with a first port fluidically connected via a first distributor to the first bed support screen; c) a second end wall, secured to or integral with the side wall(s), with a second port fluidically connected via a second distributor to the second bed support screen; d) a packing port in a wall; and e) an internal bracing, secured to, or integral with, at least one of the end walls and extending into the bed chamber.

The invention relates to a method for operating a continuous downstream process, in particular a continuous bacterial inactivation process or an in-line dilution process, using a bioprocess assembly (1), the bioprocess assembly (1) having a homogenization tank (2), which has a first fluid inlet (3) and a second fluid inlet (4), the first fluid inlet is configured to introduce a concentrate stream, in particular a product concentrate stream or a buffer concentrate stream, into the homogenizing tank (2); at least one second fluid inlet (4) which is designed to introduce a diluent stream, in particular a product diluent stream or a buffer diluent stream, into the homogenization tank (2); a fluid outlet (5), which is designed to discharge a liquid flow from the homogenizing tank (2), the bioprocess assembly (1) having a conveying assembly (6) for conveying a fluid, which conveying assembly is associated with the first fluid inlet (3) and the second fluid inlet (4) in order to convey at least one liquid flow into the homogenizing tank (2), the homogenizing tank (2) is provided with a delivery assembly (7), and the delivery assembly is associated with the fluid outlet (5) to deliver the liquid flow out of the homogenizing tank (2), and wherein the bioprocess assembly (1) has an electronic process control device (7). According to the invention, the electronic process control device (7) adjusts the liquid level in the homogenizing tank (2) by controlling and / or adjusting the conveying assembly (6) in such a way that the liquid level does not fall below a predefined minimum liquid level in the homogenizing tank (2).

An analytical method of and system for monitoring cell status whereby excitation energy is focused to an excitation cytometry volume in a cell sample to fluoresce first and second fluorophores of a cell. Fluorescence signals from the first and second fluorescing cell fluorophores are directed to a detection subsystem. A first peak in fluorescence intensity for the first fluorophore is detected as is a second peak in fluorescence intensity for the second fluorophore. The fluorescence signals are processed to identify when the first and second peaks occur substantially simultaneously indicating the presence of a cell at the excitation cytometry volume instead of background fluorescence and in response, the cellular status of the cell is measured using the intensity of the levels of the first and second peaks.

The present invention relates to the field of bioprocessing of a cell culture, particularly on immune or naive cell cultures by a bioprocessing system in which the handling and cultivation of the patient cells and the media is simplified. Specifically, the invention provides a bioprocessing system as well as a bioreactor module for performing a bioprocess on a cell culture. The invention further provides a method for performing a bioprocess on a cell culture. The invention further provides a method for manufacturing a cell therapy product. The invention further provides uses of the bioreactor module.

A device assembly (10) comprising a single-use device (12) for sensing or influencing a parameter of a running bioprocess. The single-use device (12) is configured to have at least two different defined functional states, a first functional state being an inactive delivery state in which the single-use device (12) is sterile and inoperable according to its intended use, and a second functional state being an active use state in which the single-use device (12) is operable according to its intended use. The device assembly (10) further comprises a signalling means (34) for indicating a current functional state of the single-use device (12) to a user. Each functional state is associated with a distinct signal (38, 40, 42). The device assembly (10) is configured such that a transfer from the first functional state to any other functional state of the single-use device (12) is irreversible and / or permanently prevents the signalling means (34) from indicating the signal (38) associated with the first functional state.

The invention refers to a processing chamber (100) shaped concentric around a center axis (101). The processing chamber (100) comprises a conical frustum shaped first body section (1) with a first base side (11) and a second base side (12). The second base side (12) opens into a second body section (2). The second body section extends along the center axis (101) with a first end of the second body section (2) pointing away from the first body section (1). The first end of the second body section (2) opens into a third body section (3). The third body section extends along the center axis (101) with a first end of the third body section (3) pointing in a direction away from the second body section (2) and wherein the first end of the third body section (3) is tapering into a second end (30) of the processing chamber (100).

A modular system for providing a bioprocess device assembly including a rigid skid and grid-modules. The skid includes identical plug-in structures arranged in a regular two-dimensional grid. The grid arrangement defines two-dimensional plug-in fields. At least some of the grid-modules have a matching counterpart plug-in structure. At least some of the grid-modules, in an installed state, have a two-dimensional extension in the plane of the grid that is not greater than a standard size of the plug-in fields. At least some of the grid-modules include a connection port to receive a rigid universal flow connector of standard shape and size in a standard position and orientation. At least some of the grid-modules have an integrated fluid line through which a medium to be processed or analyzed can flow. The integrated fluid line is in flow communication with the connection port of the grid-module. The grid-modules includes a flow control grid-module.

The invention discloses a synthetic bioreactor multi-parameter magnitude online traceability method and system, and belongs to the field of biological process control. The core of the method is that working and standard double measurement channels are configured for key parameters of a reactor, deviation is calculated in real time by synchronously comparing data of the working and standard double measurement channels, and a working measurement value is corrected accordingly; meanwhile, monitoring deviation to carry out credibility early warning; and finally, precise process control is realized based on the corrected high-credibility true value. The system correspondingly comprises paired sensor groups and an intelligent processing control unit. According to the method, the metering calibration process is online and normalized, the problems of measurement misalignment and control precision reduction caused by sensor drift are effectively solved, and the stability of the synthetic biological fermentation process, the consistency of product quality and the production intelligence level are remarkably improved.

The invention relates to a method of operating a bioprocess arrangement (1) to perform at least one repetition of a bioprocess, wherein the bioprocess arrangement (1) comprises at least one replaceable electronic component (8), wherein a process control system (7) controls the bioprocess arrangement (1), using a digital model (10). It is proposed that the replaceable electronic components (8) are removed and replaced by new electronic components (9), that the process control system (7) automatically detects the removal of the replaceable electronic components (8), automatically detects the presence of the new electronic components (9) and retrieves digital representations (11) of the new electronic components (9) based on the communication with the new electronic components (9), that the process control system (7) derives from the digital representations (11) of the replaceable electronic components (8) and the new electronic components (9) a functional relationship between the replaceable electronic components (8) and the new electronic components (9), that the process control system (7) replaces the digital representations (11) of the replaceable electronic components (8) with the digital representations (11) of the new electronic components (9) based on the functional relationship thereby updating the digital model (10), and, that the process control system (7) controls the bioprocess arrangement (1) to perform the bioprocess based 20 on the updated digital model (10).

A method of operating a bioprocess arrangement to perform repetition of a bioprocess, wherein the bioprocess arrangement comprises a replaceable electronic component, wherein a process control system controls the bioprocess arrangement, using a digital model. The replaceable electronic components are removed and replaced by new electronic components, the process control system automatically detects the removal of the electronic components, automatically detects the presence of the new electronic components and retrieves digital representations of the new components, the process control system derives from the digital representations of the replaceable components and the new components a functional relationship, the process control system replaces the digital representations of the replaceable components with the digital representations of the new components based on the functional relationship thereby updating the digital model, and, that the process control system controls the bioprocess arrangement to perform the bioprocess based on the updated digital model.

A measuring cell unit (16) for a bioprocess system is shown, in particular for a biopharma process, comprising a flow pipe unit (28) with a first connection end (30) having an inlet channel (34), a second connection end (32) having an outlet channel (36) and a measuring chamber (38) between the inlet and the outlet channels (34, 36). The first and second connection ends (30, 32) are configured to connect the measuring cell unit (16) into the bioprocess system. Together, the inlet channel (34), the measuring chamber (38) and the outlet channel (36) define portions of a flow duct (40) through the measuring cell unit (16). Further, the flow pipe unit (16) comprises a sideward sensor insert hole (42) opening into the measuring chamber (38) such that a measuring sensor (26) can be inserted. The flow duct (40) further comprises a transitional portion (44) along which a cross-sectional area of the flow duct (40) increases from the inlet channel (34) towards the measuring chamber (38). Additionally, the measuring cell unit (16) comprises at least one flow deflection arm (46) extending into the transitional portion (44) and deflecting a fluid flowing through the flow duct (40). Further, a bioprocess system and a method for displacing a first fluid by a second fluid are shown.

To provide sterilization of fluid-guiding elements by ultra violet (UV) light, and more particularly to provide use of such fluid-guiding elements for bioprocessing applications.SOLUTION: There is disclosed an ultra-violet (UV) light sterilizable fluid-guiding element (100, 200, 300, 400, 500) configurable to form a part of a normally closed bioprocessing fluid system, at least a portion of the element being formed from a material which is transmissive to UV light, the at least one portion of the element including one or more surfaces (134) configured to contact and guide fluids within the closed system. The element further includes at least one UV light emitting diode (LED) (154) mounted in, on, or adjacent the at least one portion and has sufficient light output to sterilize at least the one or more surfaces (134).SELECTED DRAWING: Figure 1

The invention relates to a method for the dynamic in-line mixing of a pressurized medium (1), which contains a liquid and at least one further liquid or solid component, in a bioprocess assembly (3), the liquid and the at least one further liquid or solid component are converged in a predefined volume ratio at the inlet point (4) to form a synthetic liquid flow (5), the bioprocess assembly (3) having a pump assembly (8) with a first pump (9), the first pump (9) being arranged in the line (7) of the line assembly (6), the first pump (9) being arranged in the line (7) of the line assembly (6), and the second pump (9) being arranged in the line (7) of the line assembly (6). The first pump (9) is designed as a rotary pump (10), in particular a centrifugal rotary pump, which is designed for the dynamic inline mixing of the medium (1), the first pump (9) having a liquid inlet (9a) which forms the suction side of the pump in the specified operation and a liquid outlet (9b) which forms the pressure side of the pump in the specified operation, and wherein the first pump (9) is designed as a rotary pump (10), in particular a centrifugal rotary pump, which is designed for the dynamic inline mixing of the medium (1). The medium (1) is guided by a rotary pump (10) for dynamic in-line mixing. According to the invention, the rotary pump (10) is passed through in the opposite flow direction for dynamic in-line mixing in comparison to the specified operation.

Biomanufacturing systems include at least one bioreactor and an at least one in silico prediction algorithm that integrates with said bioreactors. The at least one in silico prediction algorithm predicts and optimizes conditions for at least one bioprocess at a large-scale based on data obtained in said bioreactors.

Aerobic microbial processes comprise culturing microbes comprising a (per)chlorate respiration pathway in a bioreactor in a bioprocess employing microbial respiration of chloroxyanions as a source of oxygen for the bioprocess, in the absence of external addition of molecular oxygen.

Containers for a plurality of bags, such as bioprocess bags, include a first container segment configured to directly contact a first plurality of bags arranged in a first layer and a second container segment configured to be attached to the first container segment. The second container segment is configured to contact a second plurality of bags arranged in a second layer. The container includes a compression layer configured to be positioned between the first plurality of bags and the second plurality of bags such that the first plurality of bags are pressed against the first container segment and the second plurality of bags are pressed against the second container segment when the first container segment is attached to the second container segment.

A configurable port fitting for a container, such as a bioprocess bag, has a septa gasket groove and two opposing locking arms. The port fitting is mounted onto the container by (i) inserting one end of the port fitting into a port in the container such that a unitary septa gasket forms a seal between the port fitting and the container and (ii) rotating the port fitting to engage the locking arms with two opposing retention clips of the container. Some port fittings are fixed port fittings that provide a fixed opening into the container. Other port fittings are port sizers that receive a valve connector that enables flow control between closed and open positions. Port fittings of different sizes enable different flow rates. Some port fittings (and other connectors) have a threaded tube barb that receives a threaded tube retainer that secures tubing in place over the barb.

The invention refers to a processing chamber (100) shaped concentric around a center axis (101). The processing chamber (100) comprises a conical frustum shaped first body section (1) with a first base side (11) and a second base side (12). The second base side (12) opens into a second body section (2). The second body section extends along the center axis (101) with a first end of the second body section (2) pointing away from the first body section (1). The first end of the second body section (2) opens into a third body section (3). The third body section extends along the center axis (101) with a first end of the third body section (3) pointing in a direction away from the second body section (2) and wherein the first end of the third body section (3) is tapering into a second end (30) of the processing chamber (100).

The invention relates to a Method of operating a process arrangement (1), in particular bioprocess arrangement, wherein the method comprises a separation of two phases of an aqueous-two-phase system (2). It is proposed that the process arrangement (1) comprises a fluidized bed centrifuge (3), that the fluidized bed centrifuge (3) is used for the separation of the two phases of the aqueous-two-phase system (2), that the aqueous-two-phase system (2) is loaded into a rotating chamber (4) of the fluidized bed centrifuge (3) as a fluid flow, that due to the rotation of the chamber (4) the aqueous-two-phase system (2) is centrifuged such that a first phase (7) of the two phases is separated 10 from a second phase (8) of the two phases, and, that due to the fluid flow during the rotation of the chamber (4), the first phase (7) remains in the chamber (4) while the second phase (8) leaves the chamber (4), such that the two phases are separated.

The invention relates to a method for performing an integrity test on a test consumable (1) of a bioprocess plant using a test arrangement (2), in which a predetermined test program is applied to the test consumable (1) via the test arrangement (2) during a test routine (26) performed by a test control unit (3) of the test arrangement (2), and the integrity state is derived from the process data acquired during the test routine (26). It is proposed that, during the test routine (26), early process data (27) generated by a test sensor arrangement (4) of the test arrangement (2) in an early stage of the test program is used to determine the integrity state of the test consumable (1) by associating early process data (27) with a system model (29) of a reference module (30) in an association step (28), the reference module (30) emulates a test consumable (1), in particular a complete test consumable (1), taking into account only predetermined process data.

The present application relates to the field of environmental monitoring, and more particularly to a marine ecological environment multi-source monitoring and early warning method based on digital twinning, comprising the following steps: forming a mapping of physical sensing devices, virtual twin nodes and spatial grid units; predicting the evolution results of the phase field at multiple future time steps to obtain a key element prediction dataset; performing cluster-by-cluster simulation through clustering of the obtained ecological anomaly event cluster set and all anomaly feature lists included in each event cluster, and completing monitoring and early warning according to the simulation results. The present application defines biological elements and non-biological elements in the marine ecological system as phase field variables in a unified grid-by-grid manner, and introduces an influence term of biological activity into the source-sink term of non-biological phase field variables, thereby achieving deep two-way coupling of biological processes and environmental processes. A prediction control objective function is referenced to real-time monitoring data, and the key parameters of the phase field evolution model are dynamically adjusted by minimizing the mean square error between the predicted value and the real-time monitoring value.

A support housing for a flexible bioprocess bag, the housing comprising at least one side wall having a first segment and a second segment, the first segment movable in relation to the second segment between an open and a closed position, wherein at least a supporting part of the housing is translatable from an operating position to a bag loading position, wherein the supporting part comprises one or more retainers for holding the flexible bioprocess bag upon re-translation of the supporting part to the operating position and movement of the first segment to the closed position, and wherein the first segment comprises a first drive unit for connecting and driving a first mixing unit in the flexible bioprocess bag.