92 results about "Bioprocess" patented technology

The invention discloses a coal bed gas biological yield increase effect dynamic monitoring and evaluation method which comprises the following steps: arranging a multi-parameter sensor in a target coal bed gas well site, and collecting background data before bacterium injection; injecting a microbial bacterial solution and a nutrient solution into the coal seam according to the experimental design, and recording parameter information in the injection process; periodically collecting a gas production sample, and analyzing gas components; analyzing microbial community structure and abundance change in the water sample; combining the gas generation rate, the isotope characteristics and the microflora analysis result to identify a gas production path and calibrate a key functional flora; and constructing a mathematical model containing microbial activity, coal quality conversion and gas production efficiency, forming an evaluation index system, and outputting field process optimization suggestions based on the model. According to the method, a scientific basis is provided for dynamic optimization of the yield increasing process, and compared with a traditional laboratory method, the method can achieve systematic analysis of the in-situ microorganism process and quantitative evaluation of the gas production effect, and has good field adaptability and popularization value.

The utility model discloses a table type glass bioreactor and relates to the technical field of bioreactors. The bioreactor comprises a bioreactor body, wherein a sealing cover is arranged at the top of the bioreactor body. The moving mechanism can drive the stirring mechanism to rotate while moving up and down, so that the stirring mechanism is in a working state of rotating while moving up and down in the bioreactor body, and high-temperature liquid positioned below the bioreactor body can flow to an upper area; meanwhile, the liquid with lower temperature above the bioreactor body flows to the area with higher temperature below the bioreactor body, so that the temperature gradient of different height positions in the bioreactor body is broken, even in the area far away from the heating plate, the uniform distribution of the temperature can be realized, and the consistency of the temperature in the bioreactor is improved; and biological processes such as cell culture and fermentation are facilitated.

The invention discloses a bioprocess chromatography column comprising: a) a bed chamber delimited by at least one side wall, a first bed support screen and a second bed support screen; b) a first end wall, secured to or integral with the side wall(s), with a first port fluidically connected via a first distributor to the first bed support screen; c) a second end wall, secured to or integral with the side wall(s), with a second port fluidically connected via a second distributor to the second bed support screen; d) a packing port in a wall; and e) an internal bracing, secured to, or integral with, at least one of the end walls and extending into the bed chamber.

An analytical method of and system for monitoring cell status whereby excitation energy is focused to an excitation cytometry volume in a cell sample to fluoresce first and second fluorophores of a cell. Fluorescence signals from the first and second fluorescing cell fluorophores are directed to a detection subsystem. A first peak in fluorescence intensity for the first fluorophore is detected as is a second peak in fluorescence intensity for the second fluorophore. The fluorescence signals are processed to identify when the first and second peaks occur substantially simultaneously indicating the presence of a cell at the excitation cytometry volume instead of background fluorescence and in response, the cellular status of the cell is measured using the intensity of the levels of the first and second peaks.

A modular system for providing a bioprocess device assembly including a rigid skid and grid-modules. The skid includes identical plug-in structures arranged in a regular two-dimensional grid. The grid arrangement defines two-dimensional plug-in fields. At least some of the grid-modules have a matching counterpart plug-in structure. At least some of the grid-modules, in an installed state, have a two-dimensional extension in the plane of the grid that is not greater than a standard size of the plug-in fields. At least some of the grid-modules include a connection port to receive a rigid universal flow connector of standard shape and size in a standard position and orientation. At least some of the grid-modules have an integrated fluid line through which a medium to be processed or analyzed can flow. The integrated fluid line is in flow communication with the connection port of the grid-module. The grid-modules includes a flow control grid-module.

The invention discloses a synthetic bioreactor multi-parameter magnitude online traceability method and system, and belongs to the field of biological process control. The core of the method is that working and standard double measurement channels are configured for key parameters of a reactor, deviation is calculated in real time by synchronously comparing data of the working and standard double measurement channels, and a working measurement value is corrected accordingly; meanwhile, monitoring deviation to carry out credibility early warning; and finally, precise process control is realized based on the corrected high-credibility true value. The system correspondingly comprises paired sensor groups and an intelligent processing control unit. According to the method, the metering calibration process is online and normalized, the problems of measurement misalignment and control precision reduction caused by sensor drift are effectively solved, and the stability of the synthetic biological fermentation process, the consistency of product quality and the production intelligence level are remarkably improved.

A measuring cell unit (16) for a bioprocess system is shown, in particular for a biopharma process, comprising a flow pipe unit (28) with a first connection end (30) having an inlet channel (34), a second connection end (32) having an outlet channel (36) and a measuring chamber (38) between the inlet and the outlet channels (34, 36). The first and second connection ends (30, 32) are configured to connect the measuring cell unit (16) into the bioprocess system. Together, the inlet channel (34), the measuring chamber (38) and the outlet channel (36) define portions of a flow duct (40) through the measuring cell unit (16). Further, the flow pipe unit (16) comprises a sideward sensor insert hole (42) opening into the measuring chamber (38) such that a measuring sensor (26) can be inserted. The flow duct (40) further comprises a transitional portion (44) along which a cross-sectional area of the flow duct (40) increases from the inlet channel (34) towards the measuring chamber (38). Additionally, the measuring cell unit (16) comprises at least one flow deflection arm (46) extending into the transitional portion (44) and deflecting a fluid flowing through the flow duct (40). Further, a bioprocess system and a method for displacing a first fluid by a second fluid are shown.

To provide sterilization of fluid-guiding elements by ultra violet (UV) light, and more particularly to provide use of such fluid-guiding elements for bioprocessing applications.SOLUTION: There is disclosed an ultra-violet (UV) light sterilizable fluid-guiding element (100, 200, 300, 400, 500) configurable to form a part of a normally closed bioprocessing fluid system, at least a portion of the element being formed from a material which is transmissive to UV light, the at least one portion of the element including one or more surfaces (134) configured to contact and guide fluids within the closed system. The element further includes at least one UV light emitting diode (LED) (154) mounted in, on, or adjacent the at least one portion and has sufficient light output to sterilize at least the one or more surfaces (134).SELECTED DRAWING: Figure 1

Aerobic microbial processes comprise culturing microbes comprising a (per)chlorate respiration pathway in a bioreactor in a bioprocess employing microbial respiration of chloroxyanions as a source of oxygen for the bioprocess, in the absence of external addition of molecular oxygen.

Containers for a plurality of bags, such as bioprocess bags, include a first container segment configured to directly contact a first plurality of bags arranged in a first layer and a second container segment configured to be attached to the first container segment. The second container segment is configured to contact a second plurality of bags arranged in a second layer. The container includes a compression layer configured to be positioned between the first plurality of bags and the second plurality of bags such that the first plurality of bags are pressed against the first container segment and the second plurality of bags are pressed against the second container segment when the first container segment is attached to the second container segment.

The invention refers to a processing chamber (100) shaped concentric around a center axis (101). The processing chamber (100) comprises a conical frustum shaped first body section (1) with a first base side (11) and a second base side (12). The second base side (12) opens into a second body section (2). The second body section extends along the center axis (101) with a first end of the second body section (2) pointing away from the first body section (1). The first end of the second body section (2) opens into a third body section (3). The third body section extends along the center axis (101) with a first end of the third body section (3) pointing in a direction away from the second body section (2) and wherein the first end of the third body section (3) is tapering into a second end (30) of the processing chamber (100).

The present application relates to the field of environmental monitoring, and more particularly to a marine ecological environment multi-source monitoring and early warning method based on digital twinning, comprising the following steps: forming a mapping of physical sensing devices, virtual twin nodes and spatial grid units; predicting the evolution results of the phase field at multiple future time steps to obtain a key element prediction dataset; performing cluster-by-cluster simulation through clustering of the obtained ecological anomaly event cluster set and all anomaly feature lists included in each event cluster, and completing monitoring and early warning according to the simulation results. The present application defines biological elements and non-biological elements in the marine ecological system as phase field variables in a unified grid-by-grid manner, and introduces an influence term of biological activity into the source-sink term of non-biological phase field variables, thereby achieving deep two-way coupling of biological processes and environmental processes. A prediction control objective function is referenced to real-time monitoring data, and the key parameters of the phase field evolution model are dynamically adjusted by minimizing the mean square error between the predicted value and the real-time monitoring value.

In some variations, a UV-sterilizable bioreactor system comprises: a chamber configured to carry out a reaction; a component configured for introducing a gas into the chamber; and one or more UV light sources configured to expose ultraviolet light to surfaces within the chamber. Some variations provide a method of cleaning and sterilizing a bioreactor, comprising: providing a bioreactor system with a chamber to carry out a reaction, a component for introducing a gas into the chamber, and one or more UV light sources to expose ultraviolet light to chamber surfaces; cleaning the chamber; and exposing the chamber to UV radiation to sterilize the chamber. The disclosed technology is a fundamental advance in the field of sparged bioreactors, for many types of commercial fermentations. The bioreactor may incorporate materials that are expressly incompatible with steam sterilization, which allows for the use of lower-cost materials, among many other benefits from UV sterilization.

Sterile connectors for the sterile transfer of a liquid, in particular biological, medium from a liquid container (2) into a bioprocess plant (3), in particular into a bioreactor, wherein the sterile connector (1) has two coupling devices (4a, 4b), characterized by that the coupling devices (4a, 4b) each have a housing (5a, 5b) with a housing interior (6a, 6b) and an actuating element (8a, 8b) adjustable in the housing interior (6a, 6b) forming a channel (7a, 7b), wherein the channel (7a, 7b) extends from an actuating element inlet opening (9a, 9b) to an actuating element outlet opening (10a, 10b) through the actuating element (8a, 8b), that the housing (5a) of one coupling device (4a) is mechanically connectable to the housing (5b) of the other coupling device (4b), that each housing (5a, 5b) has a fluid inlet (11a, 11b) and a fluid outlet (12a, 12b) that connect the housing interior (6a, 6b) to the environment, wherein, in the mechanically connected state of the housings (5a, 5b), the fluid outlet (12a) of one housing (5a) and the fluid inlet (11b) of the other housing (5b) are in overlapping positions, that in the mechanically connected state of the housings (5a, 5b) the actuating elements (8a, 8b) are each adjustable from a starting position in which a fluid connection between channel (7a, 7b) and actuating element inlet opening (9a, 9b) on the one hand and between channel (7a, 7b) and actuating element outlet opening (10a, 10b) on the other hand is blocked, to an operating position in which the actuating element inlet opening (9a, 9b) is in fluid communication with the fluid inlet (11a, 11b) and the actuating element outlet opening (10a, 10b) is in fluid communication with the fluid outlet (12a, 12b), that in the initial position a section of the respective actuating element (8a, 8b) hermetically seals the fluid inlet (11a, 11b) and a section of the respective actuating element (8a, 8b) seals the fluid outlet (12a, 12b) of the respective housing (5a, 5b).

Method for performing a bioprocess to obtain processed cell cultures, wherein the processed cell cultures are destined for autologous or allogenic cell therapy, wherein the bioprocess is performed on an integrated bioprocessing system, wherein the bioprocess comprises a sequence of processing steps, wherein the processing steps each comprise at least one operation, wherein the bioprocess system comprises a base structure and preconfigurable cartridges, wherein the bioprocess system performs operations of the bioprocess via an interaction of the base structure with the cartridges, wherein the cartridges and the base structure comprise matching standardized interfaces for an interaction of the base structure with the respective cartridge, wherein the bioprocess system performs at least two operations of the bioprocess inside at least two differently preconfigured cartridges by an interaction of the base structure with the cartridges via the same base structure interface and / or identical base structure interfaces and matching cartridge interfaces.

The application discloses a kind of based on carbon source-sink and biological process's preferential protection area demarcation method, comprising: obtaining the multi-source remote sensing data of research area, generates the ecological resistance surface of research area;Based on circuit theory extraction ecological corridor and ecological node, construct the static ecological network of source-corridor-node structure;For the spatio-temporal heterogeneity characteristics of ecological source, the supply and demand of carbon fixation service of ecological source are calculated;Carbon fixation service as important ecological resources, according to the characteristics of biological migration from low resource habitat to high resource habitat, compare the supply and demand ratio of carbon fixation service between ecological source, to determine the trend of ecological corridor, calculate the flow radius of ecological source carbon sink supply area, according to flow radius determine the biological flow relationship between ecological source, divide ecological source into carbon source protection area and carbon sink protection area.

The present invention relates to an integrated bioprocessing system for performing several bioprocesses on a liquid immunocellular cell culture or naive cell culture, wherein the integrated bioprocessing system (1) performs several different bioprocesses by following different protocols, the protocols defining the actions that the integrated bioprocessing system (1) performs on the cell culture for each bioprocess, the integrated bioprocessing system (1) detects at least one state of the cell culture via a sensor (6), the protocols defining the reactions performed by the integrated bioprocessing system (1) when the state of the cell culture deviates from the normal state defined in the protocol, and the integrated bioprocessing system (1) detects at least one state of the integrated bioprocessing system (1), particularly via a sensor (6). It is proposed that the protocols define the reactions performed by the integrated bioprocessing system (1) when the state of the integrated bioprocessing system (1) deviates from the normal state defined in the protocol.

The present disclosure relates to skincare compositions that include sunflower sprout extract for treating skin conditions. More specifically, novel aspects are directed to a metabolomics guided, bioprocess engineered sunflower sprout extract obtained from sunflower seeds germinated in a controlled environment where free fatty acids are broken down to create energy within cells which is important for vitality. Incorporation of the metabolomics guided bioprocess engineered sunflower sprout extract as an active in a skincare composition, working through epigenetic pathways, results in enhanced human gene expression as well as increased protein and lipid production in manner correlated with anti-aging effects. The skincare composition comprises an enhanced extract obtained from plant seeds germinated in an environment that includes an effective amount of sucrose and enriched levels of oxygen, and a cosmetically acceptable carrier.

The present disclosure provides surfactant-based compositions, methods, kits, and systems for use in cell permeabilization, analytical and diagnostic assays, polypeptide stabilization, viral inactivation, and bioprocess workflows. In certain aspects, permeabilization buffers comprising defined concentrations of nonionic surfactants in polar protic solvents permeabilize diverse cell types while preserving intracellular targets and compatibility with downstream detection chemistry and nucleic acid amplification. Related compositions include loading, blocking, running, and mountant buffers, as well as polypeptide stabilization and viral inactivation solutions formulated with the surfactants. In additional aspects, the surfactants are used in lateral flow devices, bead-based assays, and lipid nanoparticle workflows, including determination of encapsulation efficiency. The disclosure further provides methods and systems in which data generated from surfactant-containing workflows are analyzed by machine-learning processes to determine attributes of buffer performance and recommend protocol parameters.

A skincare composition and a method for ameliorating the effects of aging. The skincare composition comprises an enhanced extract obtained from plant seeds germinated in an environment that includes an effective amount of sucrose and enriched levels of oxygen, and cosmetically acceptable carrier.

A computer implemented method for determining anomalies in a bioprocess, wherein the bioprocess is performed in a plurality of bioprocess cycles in series, each cycle corresponding to a separate instance of at least a portion of the bioprocess, the plurality of bioprocess cycles comprising a current bioprocess cycle and at least one previous bioprocess cycle, the method comprising: collecting process data for the current bioprocess cycle; providing reference data for the current bioprocess cycle and for the at least one previous bioprocess cycle of the plurality of bioprocess cycles, the reference data corresponding to the process data; communicating to a user deviation and / or conformity between the current bioprocess cycle and a corresponding reference bioprocess cycle, based on the process data and the reference data.

A protection insert can be provided with a freezing cassette to secure a bioprocess bag within the cassette. The protection insert can include a crosspiece between first and second arms, and a shielding projection extending from the crosspiece. The first and second arms and the crosspiece can be configured to partially accommodate the bioprocess bag. The crosspiece and / or the shielding projection can include tube channels to direct tubes extending from the bioprocess bag. The crosspiece and / or arms can include a tube resting region, and the tube resting region can include a tube retention feature.

Containers for a plurality of bags, such as bioprocess bags, include a first container segment configured to directly contact a first plurality of bags arranged in a first layer and a second container segment configured to be attached to the first container segment. The second container segment is configured to contact a second plurality of bags arranged in a second layer. The container includes a compression layer configured to be positioned between the first plurality of bags and the second plurality of bags such that the first plurality of bags are pressed against the first container segment and the second plurality of bags are pressed against the second container segment when the first container segment is attached to the second container segment.

This item is a mixer assembly used to perform bioprocessing procedures such as mixing cell cultures and related substances.

A system for preparing fluids for bioprocessing and pharmaceutical applications uses one or more flexible bags pre-filled with solid tablets of various buffer / media / physiological fluid components. These may include salts, acids, bases, preservatives, proteins, amino acids, growth factors, small molecules, and drugs. The flexible bag contains one or more sealed or sealable openings used to add water to the flexible bag's interior and remove fluids from the flexible bag. The one or more sealed openings can also be used as outlets for removing fluids (e.g., buffers) from the flexible bag. The flexible bag can be stored in a contracted state to facilitate storage and / or transportation of the flexible bag. Multiple flexible bags can be coupled to a manifold, which has valves for selectively filling and draining the flexible bags.

The invention relates to a portable container holder for use in a bioprocess for holding and transporting a disposable container (3) which is filled with a fluid (2) and which has received a magnetically driven mixing element (4) for mixing the fluid (2), wherein the portable container holder (1) has a container receiving area (7) for receiving the disposable container (3), said receiving area having a drive location (8) where the mixing element (4) can be arranged in the disposable container (3) received in the container receiving area (7) such that the mixing element (4) can be driven by a drive unit (9) at the drive location (8). According to the invention, the portable container holder (1) has a transport securing unit (11) for securing and releasing the mixing element (4), which is arranged at the drive location (8), in its position, and the transport securing unit (11) has a magnet (10) which can be moved between a defined securing position (12), in which the mixing element (4) is secured in its position, and a defined release position (13), in which the mixing element (4) is released from its position.

A diaphragm pump for a bioprocess system comprises a pump head (1; 101; 201; 301) comprising: a common inlet (3); a common outlet (5); a plurality of pump cavities (7) each including at least one cooperating pair of one-way valves, the at least one pair of one-way valves including an inlet valve (9) and an outlet valve (11), wherein the respective inlet valves (9) are in fluid communication with the common inlet (3) and the respective outlet valves (11) are in fluid communication with the common outlet (5) and wherein a centre of the outlet valve (11) for each pump cavity is positionable above a centre of the inlet valve (9) for the same pump cavity when the diaphragm pump is oriented for use to inhibit trapped gas; and a plurality of moveable diaphragms (13) each respectively provided in a respective of said pump cavities (7) for varying a volume of the pump cavities. The system further comprises a pump drive (31) which is configured to transfer a motion to the diaphragms (13) of the pump head (1; 101; 201) for accomplishing a fluid displacement from the common inlet (3) to the common outlet (5) of the pump head (1; 101; 201; 301) as a result of said varying of the volume of the pump cavities (7).

Microorganisms and bioprocesses are provided that convert gaseous C1 containing substrates, such as syngas, producer gas, and renewable H2 combined with CO2, into nutritional and other useful bioproducts. Disclosed herein are microorganisms containing that are capable of growing on gaseous carbon dioxide, gaseous hydrogen, syngas, or combinations thereof. The application describes methods for production of glucose and the glucose polymer starch and other polysaccharides by CO2-fixing chemoautotroph strains. The invention includes a method of production wherein the organisms are cultivated microaerobically or aerobically in a bioreactor using CO2, H2 and O2 in an oxyhydrogen fermentation and methods for recovery and purification of secreted glucose, intracellular starch, and other polysaccharides.