224 results about "Bioprocess" patented technology

The application relates to a computer-implemented method for configuring and / or controlling a bioprocessing system that is configured to physically perform and / or simulate a bioprocess, a respective computer program product, a method for performing a bioprocess, a control device for controlling and / or configuring a bioprocessing system and a bioprocess. The method uses a probabilistic algorithm to determine a predictive posterior distribution for a target set of model parameter values and configure and / or control at least one state parameter of the bioprocessing system cand / or bioprocess based on the determined predictive posterior distribution. The probabilistic algorithm employs a combination of evaluation methods based on a common physical model, including a numerical simulation method, a search method for searching in a data storage storing a plurality precomputed solution-parameter sets of the physical model and an artificial intelligence based method using a physics informed artificial intelligence model (such as physics informed neural network model), which is trained based on the solutions of the physical model generated by the simulation method and the physical model.

The invention provides an automatic stirring device in the field of fluid machinery and bioreactors, focusing on enhancing fluid control efficiency and consistency in biological cultivation processes. It utilizes a flexible channel body or reactor body to facilitate the flow of nutrient solution along specific channels (such as S-shaped flexible channels or S-shaped loop channels), designed to reduce shear forces and achieve iso-level uniform flow. A power system and monitoring and control system are employed to regulate the flow of nutrient solution and monitor cultivation conditions in real-time, thus supporting various bioprocess cultivation requirements.

The present invention relates to a system for loading and unloading agents in the form of particulate solid material, used for carrying out a physical, chemical, and / or biological process in one or more tanks. The system includes: an intermediate tank, a pump, and a pneumatic conveyor. The intermediate tank and the pump are fluidically connected. For unloading a tank containing saturated or degraded solid material particles, an empty or partially empty solid material tank is fluidically connected to the intermediate tank and the tank containing saturated or degraded solid material particles. For loading a tank, a solid material tank containing unsaturated or undegraded solid material particles is fluidically connected to the intermediate tank.

The invention discloses a coal bed gas biological yield increase effect dynamic monitoring and evaluation method which comprises the following steps: arranging a multi-parameter sensor in a target coal bed gas well site, and collecting background data before bacterium injection; injecting a microbial bacterial solution and a nutrient solution into the coal seam according to the experimental design, and recording parameter information in the injection process; periodically collecting a gas production sample, and analyzing gas components; analyzing microbial community structure and abundance change in the water sample; combining the gas generation rate, the isotope characteristics and the microflora analysis result to identify a gas production path and calibrate a key functional flora; and constructing a mathematical model containing microbial activity, coal quality conversion and gas production efficiency, forming an evaluation index system, and outputting field process optimization suggestions based on the model. According to the method, a scientific basis is provided for dynamic optimization of the yield increasing process, and compared with a traditional laboratory method, the method can achieve systematic analysis of the in-situ microorganism process and quantitative evaluation of the gas production effect, and has good field adaptability and popularization value.

The application relates to a computer-implemented method for configuring and / or controlling a bioprocessing system that is configured to physically perform and / or simulate a bioprocess, a respective computer program product, a method for performing a bioprocess, a control device for controlling and / or configuring a bioprocessing system and a bioprocess. The method uses a probabilistic algorithm to determine a predictive posterior distribution for a target set of model parameter values and configure and / or control at least one state parameter of the bioprocessing system cand / or bioprocess based on the determined predictive posterior distribution. The probabilistic algorithm employs a combination of evaluation methods based on a common physical model, including a numerical simulation method, a search method for searching in a data storage storing a plurality precomputed solution-parameter sets of the physical model and an artificial intelligence based method using a physics informed artificial intelligence model (such as physics informed neural network model), which is trained based on the solutions of the physical model generated by the simulation method and the physical model.

The article is a static mixer used in a bioprocessing system used for mixing fluids.

The present invention relates to a system, method, and use for the unloading of solid material particles used in a physical, chemical, and / or biological process in a tank and for the inertization of the tank. The receiving tank and the pump are fluidically connected. The inert gas supply device is fluidically connected to the tank and to at least one injector. The at least one injector is arranged along the transport line that connects the tank to the receiving tank and is configured to inject inert gas into the transport line. The pump is configured to generate negative pressure in the receiving tank and in the tank, and the inert gas supply device is configured to insert inert gas into the tank and supply inert gas to at least one injector, to allow the displacement of solid material particles from the tank to the receiving tank.

The utility model discloses a table type glass bioreactor and relates to the technical field of bioreactors. The bioreactor comprises a bioreactor body, wherein a sealing cover is arranged at the top of the bioreactor body. The moving mechanism can drive the stirring mechanism to rotate while moving up and down, so that the stirring mechanism is in a working state of rotating while moving up and down in the bioreactor body, and high-temperature liquid positioned below the bioreactor body can flow to an upper area; meanwhile, the liquid with lower temperature above the bioreactor body flows to the area with higher temperature below the bioreactor body, so that the temperature gradient of different height positions in the bioreactor body is broken, even in the area far away from the heating plate, the uniform distribution of the temperature can be realized, and the consistency of the temperature in the bioreactor is improved; and biological processes such as cell culture and fermentation are facilitated.

The present disclosure provides methods, systems and products for controlling a bioprocess comprising a cell culture in a bioreactor, wherein the bioprocess is operated as a perfusion bioprocess the methods use a model predictive control to identify values of manipulated variables that optimise an objective function, the objective function including one or more constraints and / or one or more terms that are associated with predictions obtained from a method of simulating a chromatographic process that is coupled with the bioprocess for processing of a harvest stream of the bioprocess.

A method of manufacturing a composite sensor assembly includes: providing a port including a high surface tension thermoplastic, the port having a hollow tubular portion and a base having a polymer surface; fusing the polymer surface of the port to a wall of a polymeric bioprocess vessel so that the port communicates with an opening in the wall; and retaining a sensor unit within the hollow tubular portion of the port with an adhesive, at least a portion of the sensor unit being polymeric, wherein the port provides the sensor unit access to an interior of the polymeric bioprocess vessel.

The present disclosure relates to a system and method for continuous bioprocessing simulation utilizing parameterized and pre-validated model components. The system selects validated equations from a catalogue to construct modular unit operation models, which are then instantiated into configured instances. These instances are interconnected into an integrated flowsheet simulation, which is co-simulated to capture the transient behavior of components with different time constants. The parametrized, modular modeling approach enables rapid and robust simulation of continuous biopharmaceutical production processes by assembling simulations from pre-qualified building blocks. This facilitates efficient digital prototyping and analysis of integrated continuous bioprocess systems.

The present disclosure refers to a process for the production of C4-C10 carboxylic acid by carbon chain elongation, comprising the steps of: (i) providing a hydrophobic membrane (1) with a first side (2) and a second side (3) opposite to said first side (2); (ii) bringing a feed stream (4) comprising organic C1-C4 compounds, of which at least one is an electron donor, into contact with said first side (2) of said hydrophobic membrane (1); (iii) bringing an aqueous solution (5) comprising enzymes capable of carrying out carbon chain elongating of organic C1-C4 compounds into contact with said second side (3) of said hydrophobic membrane (1), thereby causing the organic C1-C4 compounds concentration-driven diffusion from said feed stream (4) through said hydrophobic membrane (1) to said aqueous solution (5); (iv) subjecting the organic C1-C4 compounds diffused into the aqueous solution (5) to enzymatic carbon chain elongation in the presence of the electron donor; and (v) separating the formed C4-C10 carboxylic acid from the aqueous solution (5).

To provide a method for producing 6-hydroxyhexanoic acid (6-HHA) with increased production efficiency through a bioprocess.SOLUTION: A method for producing 6-hydroxyhexanoic acid comprises culturing a bacterium belonging to the genus Shewanella in a medium containing adipic acid under metabolically controlled conditions, where the bacterium has a carboxylic acid reductase and an aldehyde reductase.SELECTED DRAWING: None

The invention discloses a bioprocess chromatography column comprising: a) a bed chamber delimited by at least one side wall, a first bed support screen and a second bed support screen; b) a first end wall, secured to or integral with the side wall(s), with a first port fluidically connected via a first distributor to the first bed support screen; c) a second end wall, secured to or integral with the side wall(s), with a second port fluidically connected via a second distributor to the second bed support screen; d) a packing port in a wall; and e) an internal bracing, secured to, or integral with, at least one of the end walls and extending into the bed chamber.

The invention relates to a method for operating a continuous downstream process, in particular a continuous bacterial inactivation process or an in-line dilution process, using a bioprocess assembly (1), the bioprocess assembly (1) having a homogenization tank (2), which has a first fluid inlet (3) and a second fluid inlet (4), the first fluid inlet is configured to introduce a concentrate stream, in particular a product concentrate stream or a buffer concentrate stream, into the homogenizing tank (2); at least one second fluid inlet (4) which is designed to introduce a diluent stream, in particular a product diluent stream or a buffer diluent stream, into the homogenization tank (2); a fluid outlet (5), which is designed to discharge a liquid flow from the homogenizing tank (2), the bioprocess assembly (1) having a conveying assembly (6) for conveying a fluid, which conveying assembly is associated with the first fluid inlet (3) and the second fluid inlet (4) in order to convey at least one liquid flow into the homogenizing tank (2), the homogenizing tank (2) is provided with a delivery assembly (7), and the delivery assembly is associated with the fluid outlet (5) to deliver the liquid flow out of the homogenizing tank (2), and wherein the bioprocess assembly (1) has an electronic process control device (7). According to the invention, the electronic process control device (7) adjusts the liquid level in the homogenizing tank (2) by controlling and / or adjusting the conveying assembly (6) in such a way that the liquid level does not fall below a predefined minimum liquid level in the homogenizing tank (2).

An analytical method of and system for monitoring cell status whereby excitation energy is focused to an excitation cytometry volume in a cell sample to fluoresce first and second fluorophores of a cell. Fluorescence signals from the first and second fluorescing cell fluorophores are directed to a detection subsystem. A first peak in fluorescence intensity for the first fluorophore is detected as is a second peak in fluorescence intensity for the second fluorophore. The fluorescence signals are processed to identify when the first and second peaks occur substantially simultaneously indicating the presence of a cell at the excitation cytometry volume instead of background fluorescence and in response, the cellular status of the cell is measured using the intensity of the levels of the first and second peaks.

The present invention relates to the field of bioprocessing of a cell culture, particularly on immune or naive cell cultures by a bioprocessing system in which the handling and cultivation of the patient cells and the media is simplified. Specifically, the invention provides a bioprocessing system as well as a bioreactor module for performing a bioprocess on a cell culture. The invention further provides a method for performing a bioprocess on a cell culture. The invention further provides a method for manufacturing a cell therapy product. The invention further provides uses of the bioreactor module.

A device assembly (10) comprising a single-use device (12) for sensing or influencing a parameter of a running bioprocess. The single-use device (12) is configured to have at least two different defined functional states, a first functional state being an inactive delivery state in which the single-use device (12) is sterile and inoperable according to its intended use, and a second functional state being an active use state in which the single-use device (12) is operable according to its intended use. The device assembly (10) further comprises a signalling means (34) for indicating a current functional state of the single-use device (12) to a user. Each functional state is associated with a distinct signal (38, 40, 42). The device assembly (10) is configured such that a transfer from the first functional state to any other functional state of the single-use device (12) is irreversible and / or permanently prevents the signalling means (34) from indicating the signal (38) associated with the first functional state.

The invention refers to a processing chamber (100) shaped concentric around a center axis (101). The processing chamber (100) comprises a conical frustum shaped first body section (1) with a first base side (11) and a second base side (12). The second base side (12) opens into a second body section (2). The second body section extends along the center axis (101) with a first end of the second body section (2) pointing away from the first body section (1). The first end of the second body section (2) opens into a third body section (3). The third body section extends along the center axis (101) with a first end of the third body section (3) pointing in a direction away from the second body section (2) and wherein the first end of the third body section (3) is tapering into a second end (30) of the processing chamber (100).

A modular system for providing a bioprocess device assembly including a rigid skid and grid-modules. The skid includes identical plug-in structures arranged in a regular two-dimensional grid. The grid arrangement defines two-dimensional plug-in fields. At least some of the grid-modules have a matching counterpart plug-in structure. At least some of the grid-modules, in an installed state, have a two-dimensional extension in the plane of the grid that is not greater than a standard size of the plug-in fields. At least some of the grid-modules include a connection port to receive a rigid universal flow connector of standard shape and size in a standard position and orientation. At least some of the grid-modules have an integrated fluid line through which a medium to be processed or analyzed can flow. The integrated fluid line is in flow communication with the connection port of the grid-module. The grid-modules includes a flow control grid-module.

The invention discloses a synthetic bioreactor multi-parameter magnitude online traceability method and system, and belongs to the field of biological process control. The core of the method is that working and standard double measurement channels are configured for key parameters of a reactor, deviation is calculated in real time by synchronously comparing data of the working and standard double measurement channels, and a working measurement value is corrected accordingly; meanwhile, monitoring deviation to carry out credibility early warning; and finally, precise process control is realized based on the corrected high-credibility true value. The system correspondingly comprises paired sensor groups and an intelligent processing control unit. According to the method, the metering calibration process is online and normalized, the problems of measurement misalignment and control precision reduction caused by sensor drift are effectively solved, and the stability of the synthetic biological fermentation process, the consistency of product quality and the production intelligence level are remarkably improved.

The invention relates to a method of operating a bioprocess arrangement (1) to perform at least one repetition of a bioprocess, wherein the bioprocess arrangement (1) comprises at least one replaceable electronic component (8), wherein a process control system (7) controls the bioprocess arrangement (1), using a digital model (10). It is proposed that the replaceable electronic components (8) are removed and replaced by new electronic components (9), that the process control system (7) automatically detects the removal of the replaceable electronic components (8), automatically detects the presence of the new electronic components (9) and retrieves digital representations (11) of the new electronic components (9) based on the communication with the new electronic components (9), that the process control system (7) derives from the digital representations (11) of the replaceable electronic components (8) and the new electronic components (9) a functional relationship between the replaceable electronic components (8) and the new electronic components (9), that the process control system (7) replaces the digital representations (11) of the replaceable electronic components (8) with the digital representations (11) of the new electronic components (9) based on the functional relationship thereby updating the digital model (10), and, that the process control system (7) controls the bioprocess arrangement (1) to perform the bioprocess based 20 on the updated digital model (10).

A method for automating foam control during a bioprocess for cultivating cells in a bioreactor comprises the following steps: determining values ​​of one or more process variables of the bioprocess that are relevant for foam formation in the bioreactor; transmitting the determined values ​​as input signals to a controller; generating output signals by the controller for at least one foam control device depending on the input signals; and controlling the at least one foam control device as required by means of the output signals issued by the controller.

A method of operating a bioprocess arrangement to perform repetition of a bioprocess, wherein the bioprocess arrangement comprises a replaceable electronic component, wherein a process control system controls the bioprocess arrangement, using a digital model. The replaceable electronic components are removed and replaced by new electronic components, the process control system automatically detects the removal of the electronic components, automatically detects the presence of the new electronic components and retrieves digital representations of the new components, the process control system derives from the digital representations of the replaceable components and the new components a functional relationship, the process control system replaces the digital representations of the replaceable components with the digital representations of the new components based on the functional relationship thereby updating the digital model, and, that the process control system controls the bioprocess arrangement to perform the bioprocess based on the updated digital model.

A measuring cell unit (16) for a bioprocess system is shown, in particular for a biopharma process, comprising a flow pipe unit (28) with a first connection end (30) having an inlet channel (34), a second connection end (32) having an outlet channel (36) and a measuring chamber (38) between the inlet and the outlet channels (34, 36). The first and second connection ends (30, 32) are configured to connect the measuring cell unit (16) into the bioprocess system. Together, the inlet channel (34), the measuring chamber (38) and the outlet channel (36) define portions of a flow duct (40) through the measuring cell unit (16). Further, the flow pipe unit (16) comprises a sideward sensor insert hole (42) opening into the measuring chamber (38) such that a measuring sensor (26) can be inserted. The flow duct (40) further comprises a transitional portion (44) along which a cross-sectional area of the flow duct (40) increases from the inlet channel (34) towards the measuring chamber (38). Additionally, the measuring cell unit (16) comprises at least one flow deflection arm (46) extending into the transitional portion (44) and deflecting a fluid flowing through the flow duct (40). Further, a bioprocess system and a method for displacing a first fluid by a second fluid are shown.

To provide sterilization of fluid-guiding elements by ultra violet (UV) light, and more particularly to provide use of such fluid-guiding elements for bioprocessing applications.SOLUTION: There is disclosed an ultra-violet (UV) light sterilizable fluid-guiding element (100, 200, 300, 400, 500) configurable to form a part of a normally closed bioprocessing fluid system, at least a portion of the element being formed from a material which is transmissive to UV light, the at least one portion of the element including one or more surfaces (134) configured to contact and guide fluids within the closed system. The element further includes at least one UV light emitting diode (LED) (154) mounted in, on, or adjacent the at least one portion and has sufficient light output to sterilize at least the one or more surfaces (134).SELECTED DRAWING: Figure 1

The present invention provides a modular support container for a disposable bioprocess container, comprising a support container base, a support container pedestal, and a plurality of container boxes that are fixedly connected in sequence from bottom to top; the support container pedestal is provided with a liquid discharge plane, and the liquid discharge plane is provided with a liquid discharge port; the container box is surrounded by a plurality of container vertical plates, and the container vertical plates are hollow structures, provided with a liquid inlet and a liquid outlet, and connected to a cooling water circulation pipeline in a series or parallel manner. The modular support container for the disposable bioprocess container greatly reduces the processing cost and improves the flexibility of use in pharmaceutical factories.

The invention relates to a method for the dynamic in-line mixing of a pressurized medium (1), which contains a liquid and at least one further liquid or solid component, in a bioprocess assembly (3), the liquid and the at least one further liquid or solid component are converged in a predefined volume ratio at the inlet point (4) to form a synthetic liquid flow (5), the bioprocess assembly (3) having a pump assembly (8) with a first pump (9), the first pump (9) being arranged in the line (7) of the line assembly (6), the first pump (9) being arranged in the line (7) of the line assembly (6), and the second pump (9) being arranged in the line (7) of the line assembly (6). The first pump (9) is designed as a rotary pump (10), in particular a centrifugal rotary pump, which is designed for the dynamic inline mixing of the medium (1), the first pump (9) having a liquid inlet (9a) which forms the suction side of the pump in the specified operation and a liquid outlet (9b) which forms the pressure side of the pump in the specified operation, and wherein the first pump (9) is designed as a rotary pump (10), in particular a centrifugal rotary pump, which is designed for the dynamic inline mixing of the medium (1). The medium (1) is guided by a rotary pump (10) for dynamic in-line mixing. According to the invention, the rotary pump (10) is passed through in the opposite flow direction for dynamic in-line mixing in comparison to the specified operation.

The disclosure relates to monitoring or controlling bioprocesses by way of withdrawing discrete fluid samples from a reactor and performing on-line analysis of said sample using a fluidic manifold, for example, by way of a sensor based on label-free biomolecular interaction analysis. The disclosure relates to a system for measuring one or more analytes in discrete fluid samples from a vessel adapted to contain a fluid having one or more sensors for the measurement of the analytes in a contact volume of the fluid sample. An analysis module having at least one main sampling line in fluidic connection with the vessel and one or more pumps in fluidic connection with said main sampling line(s) and adapted to selectively pump fluid from in the main sampling line away from the vessel. At least one fluid distribution tubing in fluidic connection with the analysis module. A manifold has a body.

Biomanufacturing systems include at least one bioreactor and an at least one in silico prediction algorithm that integrates with said bioreactors. The at least one in silico prediction algorithm predicts and optimizes conditions for at least one bioprocess at a large-scale based on data obtained in said bioreactors.