142 results about "Bioprocess" patented technology

The present invention relates to a system for loading and unloading agents in the form of particulate solid material, used for carrying out a physical, chemical, and / or biological process in one or more tanks. The system includes: an intermediate tank, a pump, and a pneumatic conveyor. The intermediate tank and the pump are fluidically connected. For unloading a tank containing saturated or degraded solid material particles, an empty or partially empty solid material tank is fluidically connected to the intermediate tank and the tank containing saturated or degraded solid material particles. For loading a tank, a solid material tank containing unsaturated or undegraded solid material particles is fluidically connected to the intermediate tank.

The invention discloses a coal bed gas biological yield increase effect dynamic monitoring and evaluation method which comprises the following steps: arranging a multi-parameter sensor in a target coal bed gas well site, and collecting background data before bacterium injection; injecting a microbial bacterial solution and a nutrient solution into the coal seam according to the experimental design, and recording parameter information in the injection process; periodically collecting a gas production sample, and analyzing gas components; analyzing microbial community structure and abundance change in the water sample; combining the gas generation rate, the isotope characteristics and the microflora analysis result to identify a gas production path and calibrate a key functional flora; and constructing a mathematical model containing microbial activity, coal quality conversion and gas production efficiency, forming an evaluation index system, and outputting field process optimization suggestions based on the model. According to the method, a scientific basis is provided for dynamic optimization of the yield increasing process, and compared with a traditional laboratory method, the method can achieve systematic analysis of the in-situ microorganism process and quantitative evaluation of the gas production effect, and has good field adaptability and popularization value.

The present invention relates to a system, method, and use for the unloading of solid material particles used in a physical, chemical, and / or biological process in a tank and for the inertization of the tank. The receiving tank and the pump are fluidically connected. The inert gas supply device is fluidically connected to the tank and to at least one injector. The at least one injector is arranged along the transport line that connects the tank to the receiving tank and is configured to inject inert gas into the transport line. The pump is configured to generate negative pressure in the receiving tank and in the tank, and the inert gas supply device is configured to insert inert gas into the tank and supply inert gas to at least one injector, to allow the displacement of solid material particles from the tank to the receiving tank.

The utility model discloses a table type glass bioreactor and relates to the technical field of bioreactors. The bioreactor comprises a bioreactor body, wherein a sealing cover is arranged at the top of the bioreactor body. The moving mechanism can drive the stirring mechanism to rotate while moving up and down, so that the stirring mechanism is in a working state of rotating while moving up and down in the bioreactor body, and high-temperature liquid positioned below the bioreactor body can flow to an upper area; meanwhile, the liquid with lower temperature above the bioreactor body flows to the area with higher temperature below the bioreactor body, so that the temperature gradient of different height positions in the bioreactor body is broken, even in the area far away from the heating plate, the uniform distribution of the temperature can be realized, and the consistency of the temperature in the bioreactor is improved; and biological processes such as cell culture and fermentation are facilitated.

The present disclosure provides methods, systems and products for controlling a bioprocess comprising a cell culture in a bioreactor, wherein the bioprocess is operated as a perfusion bioprocess the methods use a model predictive control to identify values of manipulated variables that optimise an objective function, the objective function including one or more constraints and / or one or more terms that are associated with predictions obtained from a method of simulating a chromatographic process that is coupled with the bioprocess for processing of a harvest stream of the bioprocess.

The present disclosure relates to a system and method for continuous bioprocessing simulation utilizing parameterized and pre-validated model components. The system selects validated equations from a catalogue to construct modular unit operation models, which are then instantiated into configured instances. These instances are interconnected into an integrated flowsheet simulation, which is co-simulated to capture the transient behavior of components with different time constants. The parametrized, modular modeling approach enables rapid and robust simulation of continuous biopharmaceutical production processes by assembling simulations from pre-qualified building blocks. This facilitates efficient digital prototyping and analysis of integrated continuous bioprocess systems.

To provide a method for producing 6-hydroxyhexanoic acid (6-HHA) with increased production efficiency through a bioprocess.SOLUTION: A method for producing 6-hydroxyhexanoic acid comprises culturing a bacterium belonging to the genus Shewanella in a medium containing adipic acid under metabolically controlled conditions, where the bacterium has a carboxylic acid reductase and an aldehyde reductase.SELECTED DRAWING: None

The invention discloses a bioprocess chromatography column comprising: a) a bed chamber delimited by at least one side wall, a first bed support screen and a second bed support screen; b) a first end wall, secured to or integral with the side wall(s), with a first port fluidically connected via a first distributor to the first bed support screen; c) a second end wall, secured to or integral with the side wall(s), with a second port fluidically connected via a second distributor to the second bed support screen; d) a packing port in a wall; and e) an internal bracing, secured to, or integral with, at least one of the end walls and extending into the bed chamber.

An analytical method of and system for monitoring cell status whereby excitation energy is focused to an excitation cytometry volume in a cell sample to fluoresce first and second fluorophores of a cell. Fluorescence signals from the first and second fluorescing cell fluorophores are directed to a detection subsystem. A first peak in fluorescence intensity for the first fluorophore is detected as is a second peak in fluorescence intensity for the second fluorophore. The fluorescence signals are processed to identify when the first and second peaks occur substantially simultaneously indicating the presence of a cell at the excitation cytometry volume instead of background fluorescence and in response, the cellular status of the cell is measured using the intensity of the levels of the first and second peaks.

The present invention relates to the field of bioprocessing of a cell culture, particularly on immune or naive cell cultures by a bioprocessing system in which the handling and cultivation of the patient cells and the media is simplified. Specifically, the invention provides a bioprocessing system as well as a bioreactor module for performing a bioprocess on a cell culture. The invention further provides a method for performing a bioprocess on a cell culture. The invention further provides a method for manufacturing a cell therapy product. The invention further provides uses of the bioreactor module.

The invention refers to a processing chamber (100) shaped concentric around a center axis (101). The processing chamber (100) comprises a conical frustum shaped first body section (1) with a first base side (11) and a second base side (12). The second base side (12) opens into a second body section (2). The second body section extends along the center axis (101) with a first end of the second body section (2) pointing away from the first body section (1). The first end of the second body section (2) opens into a third body section (3). The third body section extends along the center axis (101) with a first end of the third body section (3) pointing in a direction away from the second body section (2) and wherein the first end of the third body section (3) is tapering into a second end (30) of the processing chamber (100).

A modular system for providing a bioprocess device assembly including a rigid skid and grid-modules. The skid includes identical plug-in structures arranged in a regular two-dimensional grid. The grid arrangement defines two-dimensional plug-in fields. At least some of the grid-modules have a matching counterpart plug-in structure. At least some of the grid-modules, in an installed state, have a two-dimensional extension in the plane of the grid that is not greater than a standard size of the plug-in fields. At least some of the grid-modules include a connection port to receive a rigid universal flow connector of standard shape and size in a standard position and orientation. At least some of the grid-modules have an integrated fluid line through which a medium to be processed or analyzed can flow. The integrated fluid line is in flow communication with the connection port of the grid-module. The grid-modules includes a flow control grid-module.

The invention discloses a synthetic bioreactor multi-parameter magnitude online traceability method and system, and belongs to the field of biological process control. The core of the method is that working and standard double measurement channels are configured for key parameters of a reactor, deviation is calculated in real time by synchronously comparing data of the working and standard double measurement channels, and a working measurement value is corrected accordingly; meanwhile, monitoring deviation to carry out credibility early warning; and finally, precise process control is realized based on the corrected high-credibility true value. The system correspondingly comprises paired sensor groups and an intelligent processing control unit. According to the method, the metering calibration process is online and normalized, the problems of measurement misalignment and control precision reduction caused by sensor drift are effectively solved, and the stability of the synthetic biological fermentation process, the consistency of product quality and the production intelligence level are remarkably improved.

A measuring cell unit (16) for a bioprocess system is shown, in particular for a biopharma process, comprising a flow pipe unit (28) with a first connection end (30) having an inlet channel (34), a second connection end (32) having an outlet channel (36) and a measuring chamber (38) between the inlet and the outlet channels (34, 36). The first and second connection ends (30, 32) are configured to connect the measuring cell unit (16) into the bioprocess system. Together, the inlet channel (34), the measuring chamber (38) and the outlet channel (36) define portions of a flow duct (40) through the measuring cell unit (16). Further, the flow pipe unit (16) comprises a sideward sensor insert hole (42) opening into the measuring chamber (38) such that a measuring sensor (26) can be inserted. The flow duct (40) further comprises a transitional portion (44) along which a cross-sectional area of the flow duct (40) increases from the inlet channel (34) towards the measuring chamber (38). Additionally, the measuring cell unit (16) comprises at least one flow deflection arm (46) extending into the transitional portion (44) and deflecting a fluid flowing through the flow duct (40). Further, a bioprocess system and a method for displacing a first fluid by a second fluid are shown.

To provide sterilization of fluid-guiding elements by ultra violet (UV) light, and more particularly to provide use of such fluid-guiding elements for bioprocessing applications.SOLUTION: There is disclosed an ultra-violet (UV) light sterilizable fluid-guiding element (100, 200, 300, 400, 500) configurable to form a part of a normally closed bioprocessing fluid system, at least a portion of the element being formed from a material which is transmissive to UV light, the at least one portion of the element including one or more surfaces (134) configured to contact and guide fluids within the closed system. The element further includes at least one UV light emitting diode (LED) (154) mounted in, on, or adjacent the at least one portion and has sufficient light output to sterilize at least the one or more surfaces (134).SELECTED DRAWING: Figure 1

Aerobic microbial processes comprise culturing microbes comprising a (per)chlorate respiration pathway in a bioreactor in a bioprocess employing microbial respiration of chloroxyanions as a source of oxygen for the bioprocess, in the absence of external addition of molecular oxygen.

Containers for a plurality of bags, such as bioprocess bags, include a first container segment configured to directly contact a first plurality of bags arranged in a first layer and a second container segment configured to be attached to the first container segment. The second container segment is configured to contact a second plurality of bags arranged in a second layer. The container includes a compression layer configured to be positioned between the first plurality of bags and the second plurality of bags such that the first plurality of bags are pressed against the first container segment and the second plurality of bags are pressed against the second container segment when the first container segment is attached to the second container segment.

The invention refers to a processing chamber (100) shaped concentric around a center axis (101). The processing chamber (100) comprises a conical frustum shaped first body section (1) with a first base side (11) and a second base side (12). The second base side (12) opens into a second body section (2). The second body section extends along the center axis (101) with a first end of the second body section (2) pointing away from the first body section (1). The first end of the second body section (2) opens into a third body section (3). The third body section extends along the center axis (101) with a first end of the third body section (3) pointing in a direction away from the second body section (2) and wherein the first end of the third body section (3) is tapering into a second end (30) of the processing chamber (100).

The invention relates to a Method of operating a process arrangement (1), in particular bioprocess arrangement, wherein the method comprises a separation of two phases of an aqueous-two-phase system (2). It is proposed that the process arrangement (1) comprises a fluidized bed centrifuge (3), that the fluidized bed centrifuge (3) is used for the separation of the two phases of the aqueous-two-phase system (2), that the aqueous-two-phase system (2) is loaded into a rotating chamber (4) of the fluidized bed centrifuge (3) as a fluid flow, that due to the rotation of the chamber (4) the aqueous-two-phase system (2) is centrifuged such that a first phase (7) of the two phases is separated 10 from a second phase (8) of the two phases, and, that due to the fluid flow during the rotation of the chamber (4), the first phase (7) remains in the chamber (4) while the second phase (8) leaves the chamber (4), such that the two phases are separated.

The invention relates to a method for performing an integrity test on a test consumable (1) of a bioprocess plant using a test arrangement (2), in which a predetermined test program is applied to the test consumable (1) via the test arrangement (2) during a test routine (26) performed by a test control unit (3) of the test arrangement (2), and the integrity state is derived from the process data acquired during the test routine (26). It is proposed that, during the test routine (26), early process data (27) generated by a test sensor arrangement (4) of the test arrangement (2) in an early stage of the test program is used to determine the integrity state of the test consumable (1) by associating early process data (27) with a system model (29) of a reference module (30) in an association step (28), the reference module (30) emulates a test consumable (1), in particular a complete test consumable (1), taking into account only predetermined process data.

The present application relates to the field of environmental monitoring, and more particularly to a marine ecological environment multi-source monitoring and early warning method based on digital twinning, comprising the following steps: forming a mapping of physical sensing devices, virtual twin nodes and spatial grid units; predicting the evolution results of the phase field at multiple future time steps to obtain a key element prediction dataset; performing cluster-by-cluster simulation through clustering of the obtained ecological anomaly event cluster set and all anomaly feature lists included in each event cluster, and completing monitoring and early warning according to the simulation results. The present application defines biological elements and non-biological elements in the marine ecological system as phase field variables in a unified grid-by-grid manner, and introduces an influence term of biological activity into the source-sink term of non-biological phase field variables, thereby achieving deep two-way coupling of biological processes and environmental processes. A prediction control objective function is referenced to real-time monitoring data, and the key parameters of the phase field evolution model are dynamically adjusted by minimizing the mean square error between the predicted value and the real-time monitoring value.

A support housing for a flexible bioprocess bag, the housing comprising at least one side wall having a first segment and a second segment, the first segment movable in relation to the second segment between an open and a closed position, wherein at least a supporting part of the housing is translatable from an operating position to a bag loading position, wherein the supporting part comprises one or more retainers for holding the flexible bioprocess bag upon re-translation of the supporting part to the operating position and movement of the first segment to the closed position, and wherein the first segment comprises a first drive unit for connecting and driving a first mixing unit in the flexible bioprocess bag.

In some variations, a UV-sterilizable bioreactor system comprises: a chamber configured to carry out a reaction; a component configured for introducing a gas into the chamber; and one or more UV light sources configured to expose ultraviolet light to surfaces within the chamber. Some variations provide a method of cleaning and sterilizing a bioreactor, comprising: providing a bioreactor system with a chamber to carry out a reaction, a component for introducing a gas into the chamber, and one or more UV light sources to expose ultraviolet light to chamber surfaces; cleaning the chamber; and exposing the chamber to UV radiation to sterilize the chamber. The disclosed technology is a fundamental advance in the field of sparged bioreactors, for many types of commercial fermentations. The bioreactor may incorporate materials that are expressly incompatible with steam sterilization, which allows for the use of lower-cost materials, among many other benefits from UV sterilization.

Sterile connectors for the sterile transfer of a liquid, in particular biological, medium from a liquid container (2) into a bioprocess plant (3), in particular into a bioreactor, wherein the sterile connector (1) has two coupling devices (4a, 4b), characterized by that the coupling devices (4a, 4b) each have a housing (5a, 5b) with a housing interior (6a, 6b) and an actuating element (8a, 8b) adjustable in the housing interior (6a, 6b) forming a channel (7a, 7b), wherein the channel (7a, 7b) extends from an actuating element inlet opening (9a, 9b) to an actuating element outlet opening (10a, 10b) through the actuating element (8a, 8b), that the housing (5a) of one coupling device (4a) is mechanically connectable to the housing (5b) of the other coupling device (4b), that each housing (5a, 5b) has a fluid inlet (11a, 11b) and a fluid outlet (12a, 12b) that connect the housing interior (6a, 6b) to the environment, wherein, in the mechanically connected state of the housings (5a, 5b), the fluid outlet (12a) of one housing (5a) and the fluid inlet (11b) of the other housing (5b) are in overlapping positions, that in the mechanically connected state of the housings (5a, 5b) the actuating elements (8a, 8b) are each adjustable from a starting position in which a fluid connection between channel (7a, 7b) and actuating element inlet opening (9a, 9b) on the one hand and between channel (7a, 7b) and actuating element outlet opening (10a, 10b) on the other hand is blocked, to an operating position in which the actuating element inlet opening (9a, 9b) is in fluid communication with the fluid inlet (11a, 11b) and the actuating element outlet opening (10a, 10b) is in fluid communication with the fluid outlet (12a, 12b), that in the initial position a section of the respective actuating element (8a, 8b) hermetically seals the fluid inlet (11a, 11b) and a section of the respective actuating element (8a, 8b) seals the fluid outlet (12a, 12b) of the respective housing (5a, 5b).

Method for performing a bioprocess to obtain processed cell cultures, wherein the processed cell cultures are destined for autologous or allogenic cell therapy, wherein the bioprocess is performed on an integrated bioprocessing system, wherein the bioprocess comprises a sequence of processing steps, wherein the processing steps each comprise at least one operation, wherein the bioprocess system comprises a base structure and preconfigurable cartridges, wherein the bioprocess system performs operations of the bioprocess via an interaction of the base structure with the cartridges, wherein the cartridges and the base structure comprise matching standardized interfaces for an interaction of the base structure with the respective cartridge, wherein the bioprocess system performs at least two operations of the bioprocess inside at least two differently preconfigured cartridges by an interaction of the base structure with the cartridges via the same base structure interface and / or identical base structure interfaces and matching cartridge interfaces.

The invention relates to a method for performing a bioprocess on immune or naive cell culture(s) to obtain processed cell culture(s) on a semi-automated bioprocessing system (1), comprising a primary workstation (2) and performing a workflow, the workflow comprising a plurality of unit operations performed by the primary workstation (2), wherein the primary workstation (2) comprises a unit operation interface (4), wherein the unit operations are performed by an interaction of the unit operation interface (4) with at least one cartridge (5), wherein a first unit operation (16) and a second unit operation (17) of the plurality of unit operations are performed by the unit operation interface (4) with different cartridge configurations (13), wherein between the first and the second unit operation (16, 17) the cartridge configuration (13) is manually reconfigured in a reconfiguration step, wherein the manual reconfiguration in the reconfiguration step is supported by the bioprocessing system (1).

The application discloses a kind of based on carbon source-sink and biological process's preferential protection area demarcation method, comprising: obtaining the multi-source remote sensing data of research area, generates the ecological resistance surface of research area;Based on circuit theory extraction ecological corridor and ecological node, construct the static ecological network of source-corridor-node structure;For the spatio-temporal heterogeneity characteristics of ecological source, the supply and demand of carbon fixation service of ecological source are calculated;Carbon fixation service as important ecological resources, according to the characteristics of biological migration from low resource habitat to high resource habitat, compare the supply and demand ratio of carbon fixation service between ecological source, to determine the trend of ecological corridor, calculate the flow radius of ecological source carbon sink supply area, according to flow radius determine the biological flow relationship between ecological source, divide ecological source into carbon source protection area and carbon sink protection area.

The present invention relates to an integrated bioprocessing system for performing several bioprocesses on a liquid immunocellular cell culture or naive cell culture, wherein the integrated bioprocessing system (1) performs several different bioprocesses by following different protocols, the protocols defining the actions that the integrated bioprocessing system (1) performs on the cell culture for each bioprocess, the integrated bioprocessing system (1) detects at least one state of the cell culture via a sensor (6), the protocols defining the reactions performed by the integrated bioprocessing system (1) when the state of the cell culture deviates from the normal state defined in the protocol, and the integrated bioprocessing system (1) detects at least one state of the integrated bioprocessing system (1), particularly via a sensor (6). It is proposed that the protocols define the reactions performed by the integrated bioprocessing system (1) when the state of the integrated bioprocessing system (1) deviates from the normal state defined in the protocol.

The present disclosure relates to skincare compositions that include sunflower sprout extract for treating skin conditions. More specifically, novel aspects are directed to a metabolomics guided, bioprocess engineered sunflower sprout extract obtained from sunflower seeds germinated in a controlled environment where free fatty acids are broken down to create energy within cells which is important for vitality. Incorporation of the metabolomics guided bioprocess engineered sunflower sprout extract as an active in a skincare composition, working through epigenetic pathways, results in enhanced human gene expression as well as increased protein and lipid production in manner correlated with anti-aging effects. The skincare composition comprises an enhanced extract obtained from plant seeds germinated in an environment that includes an effective amount of sucrose and enriched levels of oxygen, and a cosmetically acceptable carrier.

Methods, systems, and apparatus, including computer programs encoded on computer storage media, for a computing platform that supports bioprocess design, execution, analysis, and technology transfer. One of the methods includes receiving, by a bioinformatics platform from a production facility, a facilities specification that represents properties and capabilities of each item of equipment of a plurality of items of equipment at the production facility. A selection of a recipe to be transferred to the production facility as a production plan is received. A matching process is performed between unit operations of the selected recipe and equipment represented by the facilities specification to convert each equipment-agnostic unit operation of the recipe into an equipment-specific production task to be performed by a particular item of equipment in the production facility. A production plan is generated having a plurality of equipment-specific production tasks for the production facility.