The invention relates to the field of biological
environmental protection, in particular to a heterotrophic
nitrification immobilized
engineering bacterium for efficient
denitrification and a preparation method and application thereof.According to the heterotrophic
nitrification immobilized
engineering bacterium for efficient
denitrification and the preparation method and application of the heterotrophic
nitrification immobilized
engineering bacterium,
polyethylene glycol-
glucan two-aqueous-phase concentration and
trehalose protection liquid are adopted for synergistic effect, the
bottleneck of
cell damage caused by a traditional
flocculation method can be avoided,
trehalose molecules can wrap bacterial cells, and the bacterial cells can be effectively degraded; the immobilized particles can
resist osmotic pressure impact and
pollutant invasion, the
survival rate of thalli is higher than 95%, so that the immobilized particles always keep ultrahigh denitrifying
enzyme activity in various
sewage environments,
honeycomb-shaped pores are formed in an
ethyl cellulose matrix, a microenvironment with a high specific surface area is provided for thalli attachment, and the built-in nano
zeolite continuously and slowly releases Cu < 2 + > and Zn < 2 + > trace elements, so that the immobilized particles can be recycled. A
thallus metabolism enzyme system is synergistically activated, and the
denitrification pathway function is enhanced; the
chitosan-polydopamine outer
coating layer strongly captures the
nitrogen-containing pollutants, so that the removal efficiency of the
nitrogen-containing pollutants is improved; through triple protection, the density of unit granular
bacteria is up to 109 cells / cm, the service life is prolonged, and the denitrification performance is improved by more than 40%.