45 results about "Microbial metabolite" patented technology

The invention belongs to the technical field of water treatment, and particularly provides a nitrogen-oxygen co-doped hypha / spruce composite adsorption material and a copper-containing sewage treatment method. According to the invention, the copper-containing sewage is pretreated by using the solid fermentation product, and then the copper-containing sewage is post-treated by using the nitrogen-oxygen co-doped hypha / spruce composite adsorption material prepared under specific conditions; wherein in the pretreatment stage, microbial metabolites in a solid fermentation product preliminarily capture free copper ions (Cu < 2 + >) through complexation, the toxicity of the sewage is reduced, and meanwhile, the copper ions are partially converted into a form which is easier to adsorb. In the post-treatment stage, the nitrogen-oxygen co-doped hypha / spruce composite adsorption material plays a key role, and efficient interception and adsorption of copper ions are achieved. In conclusion, the treatment method for the copper-containing sewage comprises a pretreatment stage and a post-treatment stage, the two stages are in synergistic interaction, and finally the removal rate of the heavy metal copper can be effectively increased.

The application discloses application of undecyl-prodigiosin in inhibition of a root rot pathogen (Fusarium oxysporum) of Salvia miltiorrhiza, and belongs to the technical field of microbial pesticides. 25 H 33 The compound has a molecular formula of C 50 N3O, is derived from Streptomyces aurantiacus YS-4, has a concentration-dependent inhibitory effect on the Fusarium oxysporum, an EC of 33.1 μg / mL, and an inhibition rate of 83.3% at 400 μg / mL. The application further provides a preparation containing the compound, wherein the concentration of the compound is not less than 20 μg / mL, and the concentration of the target area after application is controlled in a range of 20 μg / mL to 400 μg / mL, so that the root rot of Salvia miltiorrhiza can be effectively prevented and treated. As a natural microbial metabolite, the compound is environment-friendly, has no residue, and is not easy to produce drug resistance, solves the pollution problem of chemical prevention and treatment, and provides a new scheme for green prevention and treatment of the root rot of Salvia miltiorrhiza.

The invention discloses a targeted nutrient source mining method based on beef cattle intestinal type-host gene interaction, particularly relates to the field of biological information data processing, and is used for solving the problems that existing nutrient source development lacks molecular mechanism verification and is poor in targeting. The method comprises the following steps: firstly, dividing nutrition-associated intestinal types based on microbiome and transcriptome data, and constructing a co-expression network to screen host co-expression specific genes driven by the specific intestinal types; then, establishing a flora metabolite ligand set and host protein receptor model, executing molecular conformation search and Gibbs free energy calculation, and screening high-activity targeting effect factors based on physical affinity; and finally, traversal matching and quantitative screening are carried out on the natural raw material liquid chromatography-mass spectrometry data by using the targeted effect factors, and a targeted nutrition source recommendation list aiming at the specific intestine type is generated. According to the method, multi-omics correlation analysis and molecular thermodynamics verification are fused, and an accurate development closed loop from micromechanism analysis to macroscopic raw material matching is constructed.

The invention discloses a method for analyzing tryptophan metabolites of intestinal microorganisms, which comprises the following steps: sample pretreatment, on-machine detection and data processing, and can be used for quantitatively analyzing 11 indole derivatives (IAA, IPA, ILA, IArA, IAM, IPYA, IAld, TRM, IET, ICA and IS) in a serum or excrement sample. According to the method, through comprehensive methodology verification, on the basis of keeping high sensitivity and accuracy, one-time analysis can be completed only in 6 minutes on a machine, and the detection efficiency is high; the TRP microbial metabolite indole metabolite detection kit can be used for detecting TRP microbial metabolite indole metabolite, covering 11 types of indole metabolites in serum and excrement samples, covering IAld, IET, IArA and other key indole derivatives related to intestinal flora, realizing relatively comprehensive detection of TRP microbial metabolite indole metabolites, and providing a new tool for revealing a complex mutual relationship between intestinal microorganisms and host health.

The invention relates to the technical field of fertilizers, and discloses a biological protein principle-based fertilizer for preventing and treating citrus liberobacter asiaticum, which is prepared from the following raw materials in parts by weight: 40 to 70 parts of fish protein peptide fermentation products, 5 to 15 parts of EM flora active liquid, 1 to 5 parts of chelated trace element solution and 10 to 20 parts of algal polysaccharide-polyglutamic acid compound. The fish protein peptide is fermented for 180 days or longer through EM bacteria. The EM flora active liquid contains lactic acid bacteria, photosynthetic bacteria, actinomycetes and saccharomycetes. The trace elements are at least two of boron, zinc, iron, manganese and molybdenum and are chelated by polyglutamic acid or sugar alcohol. The small peptide, the bacterial metabolite and the chelate element are in slow release synergy, so that rhizosphere microecology can be rebuilt, defensive enzymes can be induced, and the phenomena of huanglong, yellowing and red nose can be relieved.

The invention relates to a microbial metabolite and an application technology of the microbial metabolite in the field of drug research, in particular to a cytochalasin compound as well as a preparation method and application of the cytochalasin compound. The cytochalasin compound is a compound 1 and a compound 2 as shown in a formula I, the molecular formula of the compound 1 is C29H35NO4, and the structural formula of the compound 1 is 1 in the formula I, and the molecular formula of the compound 2 is C29H37NO3, and the structural formula of the compound 2 is 2 in the formula I. The invention further discloses a preparation method of the cytochalasin compound. Experimental results show that the two compounds show anti-tumor activity on various tumor cell lines, can effectively inhibit cell proliferation, and have the potential of serving as a cell proliferation inhibitor or an anti-tumor preparation. Compared with traditional chemical synthesis, the preparation of the target compound is achieved through the microbial fermentation technology, and the method has the advantages of being simple in operation process, short in production period, low in cost and the like, is suitable for large-scale production and has important application prospects. Formula I

The invention relates to the technical field of special fertilizer effect compositions, and particularly discloses a seedling raising and plant promoting composition for mountain mangoes and a rapid production and field planting method. The composition comprises various components such as microbial metabolite fermentation liquor dry powder, trichoderma fermentation filter residue powder, tea bran residue crushed matter, perlite loaded chitosan oligosaccharide composite particles, a kaolin-sepiolite compound and sodium alginate gel particles, and the core components are matched and coordinated to construct a stable seedling environment structure. The invention further provides a rapid production and field planting method matched with the composition, the whole process of seedling raising, bag transferring, shaping and management before outplanting is covered, and the method is suitable for intensive cultivation and efficient transplanting of mangoes under complex mountain conditions and has good technical adaptability and application prospects.

The invention discloses a potato golden nematode repellent containing plant extract and microbial metabolite, which is prepared from the following components in parts by weight: 20 to 30 parts of plant extract, 10 to 20 parts of traditional Chinese medicine extract, 5 to 10 parts of fluensulfone, 5 to 10 parts of microbial metabolite, 5 to 10 parts of rice chaff, 10 to 20 parts of kieselguhr and 3 to 5 parts of light calcium carbonate. The invention relates to the technical field of combination of botanical pesticides and chemical pesticides. According to the potato golden nematode repellent containing the plant extract and the microbial metabolite, the insect repelling effect of the repellent can be greatly improved through the synergistic effect of the plant extract, the traditional Chinese medicine extract and the microbial metabolite, and the effect that the insect repelling drug is replaced with the pollution-free plant extract and the pollution-free microbial metabolite is well achieved; the aim of greatly reducing environmental pollution is achieved, the insect repelling effect of the potato golden nematode repellent is well enhanced, and therefore the potato golden nematode repellent is very beneficial to repelling of potato golden nematode.

The present application relates to the technical fields of microbial fermentation and feed processing, and discloses a multi-strain synergistic oxygen-consuming anaerobic feed fermentation method, which comprises the following steps: inoculating a compound fermentation inoculum containing an oxygen-consuming functional flora and an anaerobic functional flora into a feed fermentation substrate with adjusted moisture content and initial pH value at one time, and the ratio of viable bacterial count is 1:1.5-1:2.5; performing first-stage oxygen-consuming fermentation on the fermentation system, maintaining oxygen supply operation until the internal center temperature naturally rises to 40-45 DEG C; then stopping the oxygen supply operation and not performing secondary inoculation, directly applying mechanical pressure to the fermentation system to perform forced compaction and exhaust and performing physical sealing, so that the system performs second-stage anaerobic fermentation until the pH value drops to a preset acidic range, and fermented feed is prepared. The process conversion triggered by one-time inoculation and natural temperature rise avoids the introduction of external miscellaneous bacteria by segmented inoculation, and a low-oxygen high-acidity environment is constructed through microbial metabolites and physical sealing.

The invention relates to the technical field of animal health, in particular to a microbial metabolite for reducing metritis and a screening method and application thereof. According to the invention, metritis of laying fowls, which is obtained by researching microorganisms related to metritis of broiler breeding chickens, is caused by complex interaction of age-related inflammatory response, microbial flora imbalance and tryptophan metabolism disorder. On the basis of 16sRNA gene sequencing, high performance liquid chromatography-mass spectrometry and other methods, a specific metabolite indole-3-acetaldehyde is screened out, and the model animal verifies that the indole-3-acetaldehyde is applied to treatment or prevention of body metritis.

The invention discloses lachnum brevipilosum and application of the lachnum brevipilosum, and belongs to the technical field of fungal biology. The strain is separated from Vaccinium dunalianum twigs, can be used as a microbial source of a natural wood flavoring material, and particularly, it is found that the strain can produce (-)-isohorn ene in microbial metabolites for the first time, so that the blank of synthesis research of microbial wood flavoring components is filled up; the application method comprises the steps of strain activation, solid fermentation culture, dynamic collection of headspace volatile matters and the like, and a fermentation product is composite fragrance containing alcohols, olefins and other components and has rich-layered wood-soil fragrance-spicy composite fragrance; the natural wood perfume is produced in a microbial fermentation mode, the problems that plant raw materials are long in growth cycle and unstable in supply can be solved, and the method has the advantages of being controllable in process, not limited by seasons and regions and high in sustainability.

The utility model discloses a multi-layer animal pesticide residue purification powder which comprises a biological activity core layer, a heavy metal chelating layer is arranged on the outer side of the biological activity core layer, an organic toxin and pesticide residue toxin decomposition layer is arranged on the outer side of the heavy metal chelating layer, a slow release layer is arranged on the outer side of the organic toxin and pesticide residue toxin decomposition layer, and the slow release layer is arranged on the outer side of the biological activity core layer. An intestinal tract adsorption layer is arranged on the outer side of the slow-release layer, a sugar-coating layer is arranged on the outer side of the intestinal tract adsorption layer, the bioactive core layer, the heavy metal chelating layer, the organic toxin and pesticide residue toxin decomposition layer, the slow-release layer, the intestinal tract adsorption layer and the sugar-coating layer form a multi-layer structure from inside to outside, and the multiple layers are matched. Microbial metabolites, heavy metals, organic toxins and pesticide residue toxins are sequentially treated from inside to outside, and mutual interference of components is avoided; the probiotics on the core layer continuously generate metabolites after colonization in intestinal tracts, so that the animals can discharge heavy metals and toxins.

The invention relates to the technical field of animal growth regulation, in particular to application of all-trans retinoic acid in regulation of oxidation resistance and digestion and metabolism capacity of Ningxiang pigs. According to the application, all-trans-retinoic acid is added into the Ningxiang pig feed, and the research on the antioxidant capacity of intestinal microorganisms and serum of the Ningxiang pig feed shows that all-trans-retinoic acid can improve the antioxidant capacity of the Ningxiang pig by enhancing the total antioxidant capacity of the Ningxiang pig, changing the diversity of intestinal flora of the Ningxiang pig and changing microbial metabolites and metabolic pathways of the intestinal flora; the biosynthesis and metabolism of amino acids are promoted, so that the oxidation resistance and digestion and metabolism capabilities of the Ningxiang pigs are enhanced.

The application discloses a construction method of a microbial-metabolite-host regulation network of summer South China calf based on Lactobacillus reuteri, and belongs to the technical field of microorganisms. The construction method comprises the following steps: collecting rumen, fecal sample and whole blood sample of the summer South China calf; obtaining microbial layer data through 16S rRNA sequencing, obtaining metabolite layer data through non-target metabolomics, and obtaining host gene / pathway layer data through whole blood transcriptomics; performing correlation analysis on multi-omics data to obtain a microbial-metabolite-host regulation correlation network. The regulation network significantly improves the growth performance of the summer South China calf, relieves diarrhea and enhances the digestive absorption function by improving the abundance of gastrointestinal beneficial bacteria, promoting the secretion of short-chain fatty acids and activating the host immune antioxidant pathway. The application realizes the precise mechanized application of probiotics by clarifying the regulation correlation among the three, and provides a mechanism-application integrated scheme for healthy breeding of calves.

The invention discloses a medicine relocation system and method based on a quantum graph Fourier convolution network. The method comprises the following steps: integrating proteins, genes, microorganisms, metabolites, drugs, diseases and multi-source associated information of the proteins, the genes, the microorganisms, the metabolites, the drugs, the diseases to construct a heterogeneous biological information network and a local subgraph network; the method comprises the following steps: innovatively designing a quantum graph Fourier convolution network, extracting spectrum characteristics and topological information of protein, gene, microorganism, metabolite, medicine and disease nodes from a heterogeneous biological information network, updating medicine and disease node information, and obtaining discriminative characteristic representation; a multi-layer sensor is used for learning and depicting medicine-disease joint feature representation, and then the unknown curative effect of the medicine is predicted. According to the method, data mining and knowledge discovery are carried out based on the multi-source heterogeneous biological information network, the quantum Fourier graph convolutional network is innovatively used for modeling the multi-source heterogeneous biological network, high-order semantic dependence and a global structure mode between drugs and diseases can be effectively extracted, the performance of a drug relocation prediction system is improved, and the system is suitable for large-scale popularization and application. And the method has good expandability and practical application prospects.

The invention belongs to the technical field of animal nutrition and feed science, and discloses an animal feed additive containing a plant extract and organic acid and application of the animal feed additive. The additive is a composite microecological preparation particle and comprises a functional carrier which is composed of plant polysaccharide with prebiotic properties and has a three-dimensional porous network structure, the plant extract microcapsules are embedded in the functional carrier and are of a core-double shell structure, and each microcapsule is provided with a pH-responsive inner shell and a microbial metabolite-responsive outer shell. The functional carrier contains one or more probiotic-oligosaccharide interpolymers which are loaded in the functional carrier and are used as intestinal in-situ organic acid generation units. According to the invention, the time and space synergistic effect of the plant extract and the in-situ generated organic acid in the animal intestinal tract is realized, and the problems of isolated action, passive release and unstable effect of the additive components in the prior art are solved.

The application discloses application of a reagent for diagnosing a neurocognitive disorder in preparation of a product for diagnosing or assisting in diagnosing the neurocognitive disorder, and relates to the technical field of biological medicine. The application finds that there is a difference in intestinal microbial metabolites between a neurocognitive disorder patient and a healthy control, and further screens out the difference intestinal microbial metabolites as a diagnostic marker of the neurocognitive disorder, which can be used for assisting in differential diagnosis of a mild neurocognitive disorder patient. The application has important significance for early diagnosis, early intervention, and in-depth understanding of the pathological mechanism of the neurocognitive disorder. The application takes feces as a sample, has the advantages of easy-to-obtain, convenient collection, large quantity of samples, low price compared with neural images, and short detection cycle compared with blood, cerebrospinal fluid and other samples, and can be used for differential diagnosis by detecting the intestinal microbial metabolite marker. A corresponding diagnostic or auxiliary diagnostic product is developed.

This invention discloses a fluorite crystal impurity leachate based on microbial metabolites and its preparation process, relating to the field of fluorite crystal impurity leachate preparation technology. The raw materials include: microbial metabolic fermentation broth, chelation solution, synergistic solution, modified glucosamine magnesium phosphate microsphere slurry, modified hydroxyapatite nanosphere slurry, modified hydroxypropyl methylcellulose ether slurry, and deionized water. In this invention, the addition of modified glucosamine magnesium phosphate microsphere slurry significantly improves the dispersibility and stability of the microspheres, enabling in-situ adsorption of free fluoride ions in the system, eliminating the inhibitory effect of fluoride ions on microbial metabolism, and simultaneously helping to stabilize the pH of the leaching system, providing a suitable environment for impurity leaching. It also synergistically enhances the solid-liquid separation effect without reducing the grade of fluorite concentrate. The addition of modified hydroxyapatite nanosphere slurry provides excellent selective adsorption capacity, preferentially adsorbing difficult-to-remove impurities such as quartz and silicates, improving the precision of deep impurity removal.

The present invention relates to delivering benefits related to maintaining a resilient microbiome on skin through a personal care composition, especially through wash off compositions like soap, body wash or shampoo. This is achieved by using starch or its microbial metabolite preferably in such compositions. It works by upregulating expression levels of Aquaporin-3, Claudin 1, Keratin- 4, Filaggrin- 1 or Desmoglein 1 on skin.

The invention discloses an ecological prevention and control method for sclerotinia rot of colza based on microbial metabolites, and relates to the technical field of agricultural biology. The method comprises the following steps: constructing a microbial team consisting of bacillus subtilis X-01, streptomyces gall YJ1 and coniothyrium minitans parasitic bacteria, wherein the microbial team respectively secretes iturinA and sclerotia to form inhibition antibiotics and parasitic enzymes; extracting and compounding the metabolites to form a synergistic network; preparing a composite carrier composed of an inner core loading layer mesoporous silica, a middle pH sensitive hydrogel layer and an outer hydrophobic ethyl cellulose layer; and accurately applying a quick-release type suspending agent in an initial flowering stage, a pH-responsive type suspending agent in a full-bloom stage and a long-acting slow-release type suspending agent in a pod stage in stages in the whole growth period. A metabolite collaborative network is delivered through a carrier system, and a staged strategy is matched, so that the problems of single strain, poor metabolite stability and short prevention and control period in the prior art are solved, and efficient, stable and ecological prevention and control of sclerotinia rot of colza in the whole growth period are realized.

This invention discloses a hierarchical porous iron-manganese-microorganism composite functional material for desulfurization of rubber asphalt flue gas, belonging to the field of environmentally friendly asphalt technology. This invention utilizes the three-level porous functional partitioning structure of the adsorbent material, using micropores for loading iron-manganese bimetallic catalytic sites, mesopores for mass transfer, and macropores for microbial immobilization. This achieves spatial separation and synergistic effect between catalytic oxidation and microbial degradation, fundamentally preventing micropore blockage by particulate matter or microbial metabolites. It effectively solves the problems of easy clogging and rapid efficiency decline in existing desulfurization materials, and is suitable for the high oil mist and high particulate matter characteristics of rubber asphalt flue gas. This invention is applicable to the efficient purification treatment of flue gas in rubber asphalt production and can be applied to the desulfurization reaction system of rubber asphalt flue gas to achieve efficient adsorption, catalytic oxidation, and deep degradation of sulfides.

The invention provides a processing method for improving flavor of braised chicken by using microbial metabolites. The method comprises the following steps: respectively carrying out amplification culture on lactobacillus plantarum and saccharomyces cerevisiae by using an improved culture medium, carrying out centrifugal filtration and sterilization to obtain a metabolite stock solution, mixing, and embedding by using beta-cyclodextrin to obtain a stabilized composite metabolite; the chicken is slaughtered, cleaned, blanched and drained, then the chicken is coated with the compound metabolite and the caramel, then the chicken is braised in marinade, and the chicken is braised with residual heat to obtain a finished product. Through the technology of improving a microbial culture medium formula, regulating and controlling metabolic conditions and directionally enriching lactic acid bacteria and saccharomycetes composite metabolites, a channel for synthesizing hexaldehyde, phenethyl alcohol, ethyl acetate and other core volatile flavor substances by microorganisms is activated, and the effects of enriching the flavor substance types of the braised chicken and enhancing the content of characteristic aroma components are achieved; and a distinct-level flavor system is constructed on the basis of materials.

The invention belongs to the technical field of biological medicine, and particularly relates to application of intestinal microbial metabolite deaminated tyrosine to preparation of a medicine for preventing or treating non-alcoholic fatty liver disease. The deaminotyrosine can be used for effectively relieving the pathological process of the non-alcoholic fatty liver disease induced by methionine-choline deficiency diet; the liver cholesterol content of a model mouse can be reduced, the color and texture of the liver can be improved, liver lipid ectopic deposition and ballooning can be remarkably reduced, and pathological changes of liver macrophage infiltration and fibrin deposition can be reduced. Therefore, the deaminotyrosine can be used for preparing the medicine for treating or preventing the non-alcoholic fatty liver disease. The invention further provides a medicine for treating the non-alcoholic fatty liver disease, and the medicine takes the deaminated tyrosine as an active component and has the advantages of safety, high efficiency and wide application prospect.

This invention relates to a novel natural microbial metabolite: Fcl-8, derived from the fermentation broth of *Xenorhabdus budapestensis* XBD8. Purified Fcl-8 is a snow-white, flocculent crystal, highly soluble in water, as shown in Figure 1. The chemical structural formula of Fcl-8 is shown in Figure 2, with the chemical formula C1. 57 H 102 N 14 O 12 With a molecular weight of 1175.78744, its typical secondary mass spectrometry information is shown in Figure 3. Fcl-8 is derived from a natural microbial metabolite, has a novel structure and high biological activity, and possesses great potential for development into a novel biopesticide.

The invention provides a method for slowing down color deterioration of dried ivy mosses during storage. The method comprises the following steps: screening qualified dried ivy mosses and removing inferior ivy mosses; spraying a compound zymophyte solution, fermenting for 8-12 hours in an environment with the temperature of 28-30 DEG C and the humidity of 50-55% until the viscosity of surface mucus reaches 150-200 mPas, and standing to remove residual liquid; atomizing and spraying coating liquid, and drying at 40 DEG C and the air speed of 0.3 m / s until the water content is 9-10%; performing low-temperature plasma treatment and low-frequency alternating magnetic field treatment in sequence; and after vacuum heat-sealing packaging, storing the product in an LED pulsed light shelf. According to the method, compound microbial fermentation and functional coating are taken as cores, double-field synergistic treatment is matched, and the effects of stabilizing color, retaining nutrition and prolonging shelf life are achieved by utilizing the suitability of microbial metabolites and dried ballonflower components, the fitting degree of a coating film structure and a tissue surface and the matching of double-field parameters and microbial characteristics; the method fundamentally solves the problems of rapid browning of the dried ballonflower, much nutrition loss, easy microorganism breeding and rapid commodity value attenuation in the traditional preservation technology.

The present invention relates to an improved method for the detection of microorganisms in complex matrices, such as paints and coatings. The method utilizes a membrane-separated system with a microbial growth zone physically isolated from a detection zone. This configuration enhances performance by reducing interference from pigments, viscosity additives, and other matrix components in paints and coatings. The membrane can allow selective diffusion of microbial metabolites while preventing bulk transfer of the sample, which can allow for accurate detection.

The invention belongs to the technical field of microbial fermentation, and discloses a method for regulating and controlling the production of n-propanol by Lactobacillus breadmucus and application of the method. The content of the n-propanol produced by Lactobacillus breadmucus is controlled by regulating and controlling the content of organic acid in Baijiu brewing; comprising the following steps: regulating and controlling the production of n-propyl alcohol by lactobacillus breadmucus through exogenously adding organic acid; the content of n-propanol in the base liquor is regulated and controlled by controlling the acidity of after-run in the liquor brewing process. Lactobacillus breadmucus is a predominant functional microorganism for producing n-propanol in the sand making turn of Maotai-flavor liquor, it is found for the first time that organic acid can significantly affect n-propanol accumulation of lactobacillus breadmucus, and based on this, the effect of regulating and controlling the n-propanol content of base liquor by controlling the acidity of after-run is verified in a pit fermentation system. The invention provides an effective strategy for regulating and controlling the content of n-propanol in white spirit production based on the interaction of microorganisms and metabolites (organic acids). The method is good in application effect, easy and convenient to operate, high in practicability and high in universality.

The invention belongs to the technical field of saline-alkali land treatment, and particularly relates to a comprehensive improvement method for soda saline-alkali land through cooperation of modified ardealite and microorganism loaded biochar. The method comprises the following steps: cleaning ardealite by using an acidic waste liquid containing organic acid to obtain modified ardealite; mixing the modified ardealite, the saline-alkaline tolerant microorganism loaded biochar and granular aggregate to prepare a composite filler; vertical holes drilled in soda saline-alkali soil in advance are filled with the composite filler, and a reaction type permeation column is constructed; water is supplied to the reaction type permeation column, calcium ions and microbial metabolites dissolved in the column are driven to laterally permeate towards peripheral soil, and sodium ions on soil colloid are replaced. According to the invention, through system integration of'material modification 'and'spatial reconstruction', an'in-situ vertical-horizontal coupled biochemical reactor 'is constructed, and the soda saline-alkali soil is effectively improved.

The invention relates to the technical field of descaling, and discloses a thermophilic acid-producing bacterium geothermal well anti-scaling and descaling composite agent and a preparation method thereof, the thermophilic acid-producing bacterium geothermal well anti-scaling and descaling composite agent comprises the following components: a thermophilic acid-producing bacterium agent, maleic anhydride grafted modified polyaspartic acid, organic phosphonate, a silicon dispersant, a corrosion inhibitor, a stabilizer, a temperature adaptive composite additive and deionized water; the composite agent disclosed by the invention not only has excellent comprehensive performance, but also has the advantages of environmental protection, practicability, long service life and the like, and the overall technical scheme is scientific and reasonable and high in practicability. Compared with an existing chemical agent, the microbial components are introduced, the dosage of the chemical agent is reduced, the environment-friendly effect is achieved, microbial metabolites can be naturally degraded, and secondary pollution cannot be caused; compared with an existing microbial agent, through component synergy, the problem of high-temperature inactivation of strains is solved, and the long-term effectiveness of the agent is improved.