152 results about "Microbial metabolite" patented technology

A biochar-based coast saline-alkali land improvement conditioner and a preparation method and application thereof are disclosed, the biochar-based coast saline-alkali land improvement conditioner comprises, by mass, 55-60 parts of biomass charcoal, 35-40 parts of humic acid, 2-4 parts of beneficial microbial agent, 1-2 parts of urea and 3-4 parts of a binder; the biochar-based coast saline-alkali land improvement conditioner has the synergistic effect of soil improvement, activation of microorganisms, enhancement of crop saltresistance, the soil improvement helps to improve soil microflora and increase the microbial activity in the soil, microbial metabolites also enhance the crop root saltresistance, crop growth feeds back to accelerate the soil improvement, a virtuous cycle system is formed, and multiplier amplification effect can be achieved compared with a single measure.

A Strain producing dynamic controlling recombinant strain and a method for preparing D-lactic acid with the recombinant strain belong to the technical field of genetically engineered agricultural microorganisms. The recombinant strain is named (Escherichia coli)B0013-070B, and is preserved in the China center for type culture collection, and the preservation number is CCTCCNO:M2012071. A lactate dehydrogenase gene promoter ldhAp in the genome of the strain is replaced with a culture environment/nutritional factors control-type promoter. Through the utilization of the recombinant strain, fermentation is conducted for 28 to 40 hours in stages at 25 to 50 DEG C, and the level of producing D-lactic acid reaches 12.5%; the optical purity lives up to 99.9%; and the chemical purity reaches to 98.4%. The dynamic controlling to the D-lactic acid dehydrogenase encoding gene expression on D-lactic acid high-producing strain B0013-070 chromosomes is conducted, so that the gene expression is controlled only through changing the fermentation temperature during the D-lactic acid production process to achieve the purpose of efficiently synthesizing D-lactic acid through taking glucose as a raw material. After simple modification, the invention can be used for producing important microbial metabolites in other industries.

The invention discloses a reverse flotation de-ashing method for lignite, and belongs to the field of microorganism application technologies and mineral processing technologies. The method includes the steps that 1, lignite origin paenibacillus polymyxa or saccharomycetes and silicate bacterium metabolism protein are added into ore pulp, then inhibitor dextrin and silicate bacterium metabolism polysaccharose are added, and afterwards, an amine collector and a foaming agent are added to be stirred for primary flotation; 2, the inhibitor dextrin and the silicate bacterium metabolism polysaccharose are added into the ore pulp after flotation again, and then the amine collector and the foaming agent are added again to be stirred for secondary flotation, so that the product in a groove is final clean coal. By the adoption of the method, conventional flotation reagents and microorganism reagents are combined for use, the separation effect of the lignite is improved, adsorption of the collector together with fine grain clay minerals and quartz is improved, floatability of the clay minerals and the quartz is also improved, and the using quantity of conventional inhibitors is reduced by inhibiting the lignite through microorganism metabolites; the obtained reverse flotation clean coal can be better used for preparing water coal slurry.

The invention relates to the technical field of livestock breeding, in particular to a deep litter system for raising cows and a method for raising cows. Biological bedding materials with the thickness of 50 cm-60 cm are arranged in the deep litter system and composed of raw materials comprising, by weight, 50-70 parts of solid cow dung obtained through solid-liquid separation after biological fermentation and high-temperature sterilization, 30-50 parts of chaff or coconut shell flour and 0.1-0.2 part of fermentation strains; the fermentation strains comprise proteases, amylases, bacillus subtilis and lactobacillus fermenti. The deep litter system for raising cows comprises the solid cow dung, so the use cost of the deep litter system is reduced; when cows are raised, beneficial microorganisms and microbial metabolites of the biological bedding materials will be eaten and absorbed by cows, and therefore the intestinal microbial environment is improved, ecological balance of intestinal flora is maintained, cow body metabolism is improved, and the feed conversion ratio is increased.

The invention discloses a method for efficiently synthesising and extracting anthocyanin in blueberry, and specifically discloses a synthesis and extraction preparation technology for blueberry anthocyanin. The method comprises the following steps: culturing blueberry cells by high hydrostatic-pressure assisted microbial fermentation, and extracting blueberry anthocyanin by ultrafiltration chromatography; breaking the cell walls of blueberry fruits by virtue of a high hydrostatic-pressure assisted microbial metabolite to adequately release anthocyanin; adding producing enzymes of L-phenylalanine, rhodotorula glutinis and the like to promote synthesis for precursors which are not converted to anthocyanin in blueberry into anthocyanin simultaneously; subjecting the fermentation liquor to treatment of centrifuging, ultrafiltration separation, chromatographic purification, vacuum freeze-drying and the like to obtain anthocyanin with the purity of 99.31%, wherein the extraction amount of anthocyanin is increased by 15-30%. The method disclosed by the invention is simple to operate, clean and harmless in generation process, low in cost, high in anthocyanin yield, high in purity, without the need of human intestinal microbial degradation, high in medical treatment health value, suitable for large-scale production application, and extremely high in market prospect.

A compound bio-preservative for coarse cereal convenient-to-process fermented wheaten food is a novel bio-preservative compounded with natural plant extract, bio-enzyme and microorganism metabolite. In cooperation with the modern micronization technology, the bio-enzyme and the microorganism metabolite serving as main agents and the natural plant extract serving as auxiliary agent are compounded into the powdery compound bio-preservative which can be easily dissolved in water and sprayed or sprinkled. The prepared compound bio-preservative for coarse cereal convenient wheaten food has stable properties and a remarkable preservation effect, is safe, cannot produce residue, and is convenient to use. All the adopted materials meet the national standard GB2760-2007, and the compound bio-preservative can be applied in the short-time storage and transportation of coarse cereal convenient-to-process fermented wheaten food under normal temperature as well as long-time storage and preservation under low temperature. The compound bio-preservative has the effect of delaying the moulding and rotting of coarse cereal convenient-to-process fermented wheaten food in storage and transportation, and has the characteristics of safety, high effectiveness, naturalness, no residue and good preservation effects.

The invention provides glycerol-producing saccharomyces cerevisiae NAU-ZH-GY1 and application thereof, belonging to the field of microbial fermentation. The fungus is separated from unprocessed honey and is identified as saccharomyces cerevisiae with a collection number of CGMCC (China General Microbiological Culture Collection Center) No. 4551. The strain is obtained through liquid fermentation; fermentative culture comprises abundant glycerol, a large amount of living saccharomyces and rich microbial metabolism substances such as amino acid, vitamins and the like; the saccharomyces cerevisiae is applied to producing a feedstuff additive at the perinatal period of a ruminant; the glycerol can be used as an energy supplement to relieve negative energy balance; by means of the saccharomyces cerevisiae and the microbial metabolism substances such as amino acid, vitamins and the like, animal gastrointestinal tract flora can be adjusted, pathogenic microorganisms can be antagonized, immunity of organisms can be improved, conversion and absorption of nutritive materials can be promoted, and production performance can be improved; and the glycerol-producing saccharomyces cerevisiae NAU-ZH-GY1 is a novel environmentally-friendly, nutritive and safe feedstuff additive with high cost performance.

The invention relates to an oily sludge treatment agent, which comprises the following components based on the mass of oily sludge: 1-4wt% of a viscosity reducer, 1-6wt% of mahogany petroleum sulfonate, 1-6wt% of sodium dodecyl benzene sulfonate, 2-6wt% of sodium silicate and 1-3wt% of an oxidant. Specifically, the viscosity reducer can effectively reduce the viscosity of oily sludge caused by long-time storage, and the stripping effect of the crude oil after viscosity reduction is remarkable. The oxidant effectively decomposes microbial metabolites wrapped on the surfaces of sludge particlesunder the action of heating and ultrasonic reaction, so that the cleaning effect of the oily sludge is improved. An emulsifying agent, a surfactant, a dispersing agent and other surfactants can effectively emulsify the crude oil to achieve the effects of solid-liquid layering, crude oil recovery and sediment solidification. The oily sludge treatment agent provided by the invention has a remarkabletreatment effect on oil field oily sludge sediment like landing mud and tank bottom mud, and the oil content of the treated sludge sediment is smaller than 2%, thus meeting the national standard.

The invention relates to the field of biological fertilizers, in particular to a high-quality biological fertilizer and a preparation method thereof. According to the invention, a secondary fermentation method and an indigenous bacteria screening application method are applied innovatively, and a safe, efficient and convenient production method is provided, a microbial environment in which harmonious symbiosis of microbial flora is implemented and indigenous bacteria are dominant flora is obtained, and continuous accumulation of microbial metabolites is promoted. The nutritional value of the biological fertilizer is increased to achieve the following three purposes including, harmlessness, humification and generation of a large number of microorganisms and metabolites of the microorganismssuch as various antibiotics and protein substances.

A fermented feed for weaned suckling pigs comprises, by weight, 60-70 parts of a solid fermented feed, 6-10 parts of wheat flour, 10-20 parts of liquid soybean peptides, 2-4 parts of a suckling pig composite premix, 1-2 parts of a nutritional additive compound package and 0.2-0.5 part of salt. The fermented feed is obtained by mixing fermentation of various microorganisms, so the mixed feed can improve the diversity of intestinal bacteria of the suckling pigs, solves the problems of intestinal flora disorder and endocrine deficiency caused by weaning stress, and allows macromolecular substances in crude proteins to be pre-digested in advance and degraded into small-molecular peptides and other nutrients; and microbial metabolites contain a large number of bioactive metabolites, so the digestion and absorption rate and the nutritional values of the feed are significantly improved. The special fermented feed for weaned suckling pigs, obtained by the mixing fermentation of various microorganisms, is rich in Bacillus subtilis, Bacillus licheniformis, Clostridium butyricum, Bacillus coagulans and various active metabolites, has good feed attracting property, can significantly increasesthe feed intake, and greatly reduces diarrhea due to degradation of the macromolecular proteins.

The invention discloses a feed fat source combination capable of improving piglet intestinal tract health. The weight ratio of coconut oil to fish oil to lard oil is 1:1:1 in the piglet feed. When the coconut oil, the fish oil and the lard oil are added to the piglet feed at the weight ratio of 1:1:1, the daily weight increment of piglets and the feed transformation rate can be obviously enhanced, the concentration of short chain fatty acid in the content of piglet ceca can be enhanced, the number of beneficial microorganisms such as bifidobacterium and lactobacillus in ceca is increased, andthe quantity of escherichia coli is lowered. Therefore, the fat source can improve the health of the intestinal tracts of the weaned piglets and enhance the birth performance of the piglets by adjusting microbial flora groups and microbial metabolites of the piglet intestinal tracts.

The invention discloses synergistic calcium superphosphate calcium coated fertilizer and a preparation method and application thereof, and belongs to the field of fertilizer application. The coated fertilizer comprises a fertilizer core and a coating layer. The preparation method comprises the steps of: performing ammoniation on powdery calcium superphosphate in a sealed roller, adding a large amount of nutrient elements, trace amount of nutrients, fermenting organic matter and a solid binder sequentially, performing granulation through rolling so as to form the fertilizer core, then sprayinga mixed solution containing a coating agent, organic phosphorus, ammonium polyphosphate and phosphorous acid to the surface of the fertilizer core uniformly by using a spray gun in a rotary drum coating machine, performing preliminary drying, spraying a mixed solution containing a plant growth regulator, seaweed extract and microbial metabolites, and performing sufficient drying so as to form thegranular coated fertilizer. The produced coated fertilizer contains a plurality of phosphorus sources and a plurality of functional substances, growth of roots and stress resistance of crops can be improved obviously, meanwhile, the coated fertilizer has the functions of quick acting and slow acting, and the utilization efficiency of the nutrients can be improved obviously.

The invention discloses an anaerobic digestion sludge dewatering method. By adopting the anaerobic digestion sludge dewatering method, secondary utilization can be performed on sludge, the sludge is prepared into a sludge activated carbon material firstly, sludge activated carbon and a biological flocculant are adopted for combined conditioning, and deep dewatering of the sludge or anaerobic digestion sludge is realized; by taking the sludge activated carbon as a skeleton structure substance, the floc structure strength of the sludge is strengthened through the hydrophobic interaction, viscousmacromolecular organic matters including humic acid and proteins and the like in a liquid phase of the anaerobic digestion sludge are removed synchronously, the filter performance of the sludge is improved, an organic load of subsequent reflux biochemical treatment is lowered; and then by further utilizing the biological flocculant for coagulation, sludge-water separation is promoted, and efficient deep dewatering of the anaerobic digestion sludge is realized. The dewatering method provided by the invention is an environment-friendly conditioning method and can improve the filtering and dewatering efficacy of the anaerobic digestion sludge specially aiming to the characteristics of small particles, high concentration of a dissolved microbial metabolite, poor filter performance and the like of the anaerobic digestion sludge.

The invention discloses a cow heat-stress resistant feed additive. Saccharomyces cerevisiae (Sc) NAU-ZH-GY1 is applicable to prepare the cow heat-stress-resistant feed additive. The cow heat-stress-resistant feed additive, which is rich in glycerin, yeast live bacteria and microbial metabolites such as amino acids, vitamins and the like, is produced under the high-permeability condition by employing a continuously feeding manner for fermentation; and the cow heat-stress resistant feed additive is capable of cooperatively perform dual efficacy of glycerin and yeast, and is capable of substantially improving lactation yield, 4% fat corrected milk yield and milk fat percentage of cow under heat-stress condition, improving blood sugar concentration, reducing somatic cell number, concentration of non-esterified fatty acid and concentration of urea nitrogen in milk, supplementing energy for cows, alleviating body weight reduction, enhancing antioxidation capability, reducing HSP70mRNA level and effectively alleviating heat stress of cows.

The present disclosure concerns methods of using novel bacterial strains of 0617-T307, 0917-T305, 0917-T306, 0917-T307, 0118-T319, 0318-T327, and 0418-T328, the cell broth and novel metabolites produced from the bacterial strains, that can inhibit the growth of a variety of microbial species for a variety of crops. The methods include use of novel, potent antimicrobial metabolites produced from the strains corresponding to compounds having Formulas (I), (II), and (III):

The present invention provides an anti-mold preservative for food made from wheat flour and processing technology thereof. The anti-mold preservative includes biological enzymes 10-70 parts, microbial metabolites 10-60 parts, natural extract 6-40 parts and dissolved water 600-900 mg/kg. The anti-mold preservative has effects in delaying mildew and rot in the storage and transportation of the food made from wheat flour with good stability, and has characteristics of safety, high efficiency, natural materials, no residue and a good fresh-keeping effect. The processing technology comprises the following steps: preparing raw materials before preparation; weighing and taking the raw materials; mixing the raw materials evenly; diluting the mixture by taking the dissolved water; and spray painting or sprinkling the diluted mixture on the surface of the food made from wheat flour. The anti-mold preservative makes it possible to simplify the technology process, reduce production costs, greatly improve the efficiency of automated production, and expand the scope of application.

The invention discloses baby toilet powder and a preparation method thereof. The baby toilet powder is composed of at least three different types of hydrophilic colloids, and each type of hydrophiliccolloid is selected from plant sporocarp extract, plant nut extract, wood secretion, seaweed extract, microbial metabolites, fruit peel extract, animal connective tissue extract, natural macromoleculecellulose gum chemically modified and its derivatives, and plant stem extract or leaf extract. The raw materials used in the baby toilet powder are widely used in the fields of foods, medicines and daily chemical and have high safety. The selected components have strong water absorption ability, can form a hydrocolloid film on the skin surface after water absorption, can lock the skin surface moisture, can isolate the urine and fecal decomposition products in the diaper, can reduce the irritation of the decomposition products to the skin, does not contain talcum powder and a glidant, reducesthe risk of infant eating and inhalation by mistake, can effectively absorb the baby's urine and sweat and is gentle and safe.

Chromogenic 3-Indoxyl choline phosphate compounds of formula (I): wherein R is selected from the group consisting of hydrogen and C1-4 alkyl, such as methyl, ethyl, propyl and butyl while R1, R2, R3, and R4 are independently selected from the group consisting of hydrogen, halogen, cyano, nitro, carboxy, amino, amino substituted with one or two C1-4 alkyl groups, aminomethyl, hydroxy, C1-4 alkoxy, carboxyalkyl, and sulphonyl. These compounds are capable of being cleaved by lecithinase C leading to products which are calorimetrically detectable. The invention provides safe and sensitive detection of potentially pathogenic bacterial activity of such microbes as Clostridium perfringens, Bacillus cereus, Bacillus anthracis, Pseudomonas aeruginosa, Listeria monocytogenes, Heliobacter pylori, Legionella pneumophila, and others in material which may contain such activity typically including physiological samples, goods for consumption, such as food and beverages, and any other potentially infected objects or articles.