Blautia sp. AUH-JLD56 and application thereof in conversion of arctigenin

A technology of AUH-JLD56 and arctigenin, which is applied in bacteria, microorganism-based methods, biochemical equipment and methods, etc., can solve the problems of low synthesis amount, inability to obtain large quantities, and great harm to human health, etc. lack of effect

Active Publication Date: 2015-02-18
HEBEI AGRICULTURAL UNIV.
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the lack of resources of the compound 3′-DMAG, there are few reports on its physiological activity. At present, there is only one literature report that 3′-DMAG not only has anti-inflammatory activity similar to AG, but also exhibits some physiological functions different from AG. , such as the affinity of 3′-DMAG to estrogen receptors at the same concentration is significantly stronger than that of AG (Molecules, 18: 1122-1127, 2013)

Method used

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  • Blautia sp. AUH-JLD56 and application thereof in conversion of arctigenin
  • Blautia sp. AUH-JLD56 and application thereof in conversion of arctigenin
  • Blautia sp. AUH-JLD56 and application thereof in conversion of arctigenin

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] 1. Isolation and cultivation of strain AUH-JLD56

[0051] (1) Collection and culture of human feces samples

[0052] Use a sterilized cotton swab to pick up fresh human feces, put it into 1 ml of fresh BHI liquid medium, and culture it in an anaerobic workstation at 37°C for 24 hours, as a microbial flora for screening specific functional microbial strains;

[0053] (2) Isolation and culture of strain AUH-JLD56

[0054] ①Single colony isolation culture

[0055] Use fresh BHI liquid medium to serially dilute the microbial flora that has been cultivated in the anaerobic workstation for 24 hours, and dilute to a concentration of 10 –1 , 10 –2 、10 –3 、10 –4 、10 –5 、10 –6 、10 –7 、10 –8 , and then 100 microliters of concentration were respectively 10 –5 、10 –6 、10 –7 、10 –8 The dilution of microbial flora was uniformly coated on the pre-prepared BHI solid medium, and the BHI solid medium coated with the dilution of microbial flora was placed in an anaerobic works...

Embodiment 2

[0076] The isolation method of bacterial strain AUH-JLD56 is the same as embodiment 1, and the application of bacterial strain AUH-JLD56 in arctigenin transformation comprises the following steps:

[0077] (1) Cultivation of strain AUH-JLD56

[0078] After freezing and thawing the human intestinal isolate strain AUH-JLD56 stored at -70°C, inoculate 15% of the inoculum into a test tube containing fresh BHI liquid medium, and culture it in an anaerobic workstation at 37°C for 20 hours. The bacterial liquid in the medium is flocculent and turbid. Then retransfer the turbid strain AUH-JLD56 in the test tube to a plastic centrifuge tube filled with fresh BHI liquid medium with 15% inoculum, and continue to cultivate in the anaerobic workstation for 18 hours as a seed solution;

[0079] (2) Substrate crude arctigenin was co-cultured with strain AUH-JLD56

[0080] In the anaerobic workstation, the seed solution of the above-mentioned pre-cultured bacterial strain AUH-JLD56 was tran...

Embodiment 3

[0086] The isolation method of bacterial strain AUH-JLD56 is the same as embodiment 1, and the application of bacterial strain AUH-JLD56 in arctigenin transformation comprises the following steps:

[0087] (1) Cultivation of strain AUH-JLD56

[0088] After freezing and thawing the human intestinal isolate strain AUH-JLD56 stored at –70°C, inoculate 18% of the inoculum into a test tube containing fresh BHI liquid medium, and culture it in an anaerobic workstation at 37°C for 24 hours. The bacterial liquid in the medium is flocculent and turbid. Then retransfer the turbid strain AUH-JLD56 in the test tube to a plastic centrifuge tube filled with fresh BHI liquid medium with 13% inoculum, and continue to cultivate in the anaerobic workstation for 20 hours as a seed solution;

[0089] (2) Substrate crude arctigenin was co-cultured with strain AUH-JLD56

[0090] In the anaerobic workstation, the seed liquid of the above-mentioned pre-cultured bacterial strain AUH-JLD56 was transf...

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Abstract

The invention discloses a Brautieria AUH-JLD56 and its application in the transformation of arctigenin, belonging to the technical field of bacteria. Blautia sp. AUH-JLD56, the preservation number is CGMCCNo.8032. Its application includes the following steps: (1) cultivation of bacterial strain AUH-JLD56; (2) co-cultivation of substrate arctigenin crude product and bacterial strain AUH-JLD56 and separation and purification of metabolites. The bacterial strain of the present invention can efficiently convert the crude arctigenin in the traditional Chinese medicine Arctium Fructus into 3′-demethyl-arctigenin, which solves the problem of lack of 3′-demethyl-arctigenin resources, and is an effective method for The research and development of microbial metabolites of pharmacologically active substances has laid the foundation.

Description

technical field [0001] The invention relates to the technical field of bacteria. Background technique [0002] Burdock ( Actium lappa ) is the dry and mature fruit of burdock, an herb of the Compositae family. It has many records in ancient Chinese medical classics. It has been used for thousands of years for anemopyretic cold, laryngogenic cough and rash diseases. Due to the low toxicity and side effects of Arctium Fructus, there have been no reports of adverse reactions in clinical application. Therefore, the research and excavation of pharmacologically active substances in Arctium Fructus has become a research hotspot at home and abroad. Lignin and volatile oil are the landmark compounds in Arctium Fructus. So far, 17 lignin compounds have been isolated from Arctium Fructus, among which Arctiin (AT) is one of the lignin components with the highest content. Studies have shown that AT can be hydrolyzed into arctigenin (AG for short) under acidic conditions or under the a...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12P17/04C12R1/01
Inventor 王秀伶刘明月于秀梅
Owner HEBEI AGRICULTURAL UNIV.
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