4021 results about "Lignin" patented technology

The invention belongs to the technical field of gene engineering, and particularly relates to application of a ZmDIR11 gene in regulating and controlling salt stress resistance of corn. After salt stress treatment, it is found that the ZmDIR11 gene mutant corn plant growth vigor and material accumulation are significantly lower than those of a wild type B73 material, an oxidative metabolism regulation path, proline accumulation, chlorophyll synthesis, lignan accumulation and a Na < + > / K < + > transport pathway are inhibited, and related metabolic indexes are significantly lower than those of the wild type B73 material. It is shown that the ZmDIR11 gene positively regulates and controls the salt-resistant metabolic pathway of the corn in the corn, and the salt tolerance of the corn is reduced after the ZmDIR11 gene is mutated. The research provides a new gene resource for corn salt-tolerant breeding by utilizing gene engineering.

The invention provides application of a Pto-C3H35 gene haplotype in evaluation of poplar lignin content, and belongs to the technical field of molecular markers. According to the invention, the haplotype of the Pto-C3H35 gene is determined according to the nucleotides of SNP1, InDel1, InDel2, InDel3 and SNP2. The invention provides a primer group and a kit for determining the gene haplotype as well as a method for determining the gene haplotype, the lignin content of the poplar can be accurately judged by determining the genotype combination of the five sites, and superior plants with low lignin content are accurately and efficiently screened in the early growth stage of the poplar; the method is applied to new variety breeding of industrial poplar artificial forests, and effectively shortens the breeding period.

The invention discloses high-thermal-conductivity carbon black and a preparation method and application thereof, and relates to the technical field of carbon black modification.The preparation method of the high-thermal-conductivity carbon black comprises the following steps that carbon black is subjected to oxidation treatment, silanization treatment and tannic acid coating treatment, and modified carbon black is obtained; preparing a graphene / boron nitride / modified carbon black multi-dimensional filler; carrying out primary modification on the graphene / boron nitride / modified carbon black multi-dimensional filler by adopting lignin, polyvinylpyrrolidone and samarium chloride, and then carrying out secondary modification by adopting 6-aminocaproic acid to obtain the high-thermal-conductivity carbon black. The high-thermal-conductivity carbon black prepared by the invention not only has good dispersity, but also can well improve the thermal conductivity and mechanical properties of a rubber matrix.

The invention relates to the field of agricultural microbial preparations, in particular to a complex microbial inoculant sustained-release granule for preventing and treating sweet potato stem nematode disease and a preparation method of the complex microbial inoculant sustained-release granule. Comprising the following components in parts by weight: 15-25 parts of paecilomyces lilacinus viable bacterium powder, 10-20 parts of bacillus thuringiensis viable bacterium powder, 10-20 parts of streptomyces viable bacterium powder, 5-15 parts of bacillus subtilis viable bacterium powder, 15-25 parts of charcoal, 5-15 parts of sodium alginate, 5-15 parts of humic acid, 5-10 parts of chitosan, 3-8 parts of calcium silicate, 1-5 parts of mannitol and 1-5 parts of sodium lignin sulfonate. Four microorganisms with complementary functions, namely paecilomyces lilacinus, bacillus thuringiensis, streptomycete and bacillus subtilis, are adopted, the sweet potato stem nematode disease is synergistically controlled through multiple action mechanisms, and the control effect and the stability are remarkably improved.

The invention belongs to the technical field of asphalt mixtures, and relates to a high-dosage RAP low-temperature hot mix plant recycled SMA-13 asphalt mixture and a preparation process thereof, and the low-temperature hot mix plant recycled SMA-13 asphalt mixture comprises the following components by weight: 50-60 parts of an asphalt mixture reclaimed material; 30-40 parts of modified steel slag aggregate; 3-5 parts of SBS modified asphalt; 0.02 to 0.06 part of a low-temperature asphalt modifier; 3-8 parts of superfine slag powder; 0.2 to 0.5 part of lignin fiber; and 1-2 parts of a self-made asphalt regenerant. The limitation of the traditional RAP doping amount is broken through, the highest doping amount reaches 70%, the steel slag recycled aggregate replaces natural aggregate, the consumption of raw materials and the emission of waste are remarkably reduced, and the steel slag recycled aggregate meets the target requirements of the new era of dual-carbon strategy and sustainable development; and the method is particularly suitable for winter construction, heavy traffic roads and green road construction in iron and steel industry areas, and has technical advancement and engineering practicability.

The invention belongs to the technical field of rubber, and discloses bio-based reinforced resin, a preparation method, a rubber composition and application. The bio-based reinforced resin is prepared from lignin, cashew nut shell oil, rosin or derivatives thereof and a vegetable fat modifier, the biochar content is up to 100%, the Mooney viscosity and MH of a sizing material are obviously reduced, and the bio-based reinforced resin has the advantages of long scorching time and high vulcanization speed in the sizing material application process.

The invention discloses a method for catalytically oxidizing lignin to depolymerize into an aviation oil intermediate by using a cellulose carbon aerogel supported copper nano-catalyst. Comprising the following steps: (1) dissolving cellulose powder into a mixed alkali solution of NaOH and urea, and stirring at low temperature to obtain a clear and transparent cellulose solution; and washing to be neutral to obtain well dispersed cellulose suspension. And (2) carrying out suction filtration molding, and freeze-drying for a period of time to obtain the cellulose aerogel. And (3) immersing in an ethanol solution of copper nitrate for ultrasonic treatment, and drying in a vacuum drying oven. And (4) carrying out high-temperature calcination in an N2 atmosphere to obtain a catalyst which is marked as Cu / CA. And (5) the catalyst can efficiently catalyze a Fenton-like reaction to oxidize a dimer model compound of lignin in acidic, neutral and alkaline environments to obtain products including benzoic acid, acetophenone, benzyl alcohol, phenol and the like, the required hydrogen peroxide concentration is low, and the catalyst has the advantages of simple process, high catalytic activity, low cost and the like, can be used for effectively degrading lignin to prepare aviation oil intermediates, and has wide application prospects. And repeated cyclic utilization is realized.

The present invention relates to novel lignin-derived compounds and compositions comprising the same and their use as redox flow battery electrolytes. The invention further provides a method for preparing said compounds and compositions as well as a redox flow battery comprising said compounds and compositions. Additionally, an assembly for carrying out the inventive method is provided.

The invention discloses a composite modified waste cooking oil biological asphalt mixture and a preparation method thereof. The asphalt mixture comprises the following substances in percentage by mass: 4-7wt% of biological asphalt, 89-92wt% of aggregate, 2-3wt% of mixed mineral powder and 0.5-1.0 wt% of lignin fiber, the biological asphalt is prepared from the following substances in percentage by mass: 50 to 58 weight percent of matrix asphalt, 18 to 20 weight percent of waste cooking oil, 15 to 18 weight percent of mixed rubber powder, 2 to 2.5 weight percent of polymer modifier, 6 to 8 weight percent of mixed resin and 1 to 1.5 weight percent of additive. The preparation method comprises the following steps: preparing biological asphalt; respectively putting the aggregates with various grades of particle sizes, the mixed mineral powder and the biological asphalt into a drying oven for heating; sequentially putting the biological asphalt and the aggregate with various grades of particle sizes into a mixing pot, and mixing; sequentially adding the mixed mineral powder and the lignin fiber into the mixing pot, and continuously mixing to obtain the composite modified waste cooking oil biological asphalt mixture. The biological asphalt is prepared in a layer-by-layer progressive mode, and the technical problem that the doping amount of the waste cooking oil and the performance of the biological asphalt are difficult to achieve balance is solved.

A method of fabricating a biochar composite product comprises thermally treating a precursor composition by heating the precursor composition according to a heating profile to provide a biochar composite product, the precursor composition comprising biochar and a natural binder other than lignin, wherein the natural binder forms a solid, porous, covalently bound carbon matrix at or above a threshold temperature, wherein the biochar composite product comprises a plurality of porous, carbonaceous particles and the solid, porous, covalently bound carbon matrix on surfaces of particles in the plurality such that neighboring particles are adhered together. The biochar composite products and methods of using the products are also provided.

The invention discloses an application of soluble lignin as a synergist for ethanol fermentation and / or improvement of stress resistance of yeast under a fermentation environment stress condition, the soluble lignin is added into an ethanol fermentation system, and the problems of pH instability, oxidative damage, heavy metal inhibition and the like in ethanol fermentation can be synchronously solved; the survival rate of yeast and the yield of ethanol are remarkably improved, the process steps are simplified, the cost is reduced, the method is suitable for ethanol industrial production of agricultural and forestry wastes and other raw materials, and a green and practical technical path is provided for efficient utilization of all components of lignocellulose. In addition, the soluble lignin disclosed by the invention can also be recycled, can still keep a relatively high synergistic effect after being recycled for multiple times, and has a wide application prospect in the field of bioethanol fermentation.

The invention relates to the technical field of water treatment, and particularly provides a polyaluminum chloride composite water treatment agent and a preparation method thereof, and the polyaluminum chloride composite water treatment agent comprises the following components in percentage by weight: 80-90% of polyaluminum chloride; 0.5%-2% of modified chitosan; 3%-8% of sodium lignosulphonate-Fe < 3 + > complex; 0.1%-0.5% of a pH regulator; wherein the modified chitosan is citric acid cross-linked chitosan, the deacetylation degree of the modified chitosan is not lower than 85%, and in the sodium lignosulphonate-Fe < 3 + > complex, the molar ratio of Fe < 3 + > to sodium lignosulphonate is 1: 3. Through the synergistic interaction of polyaluminum chloride and a natural polymer composite system, the design of an efficient, green and multifunctional water treatment agent is realized.

The invention provides loofah sponge nanocellulose hydrogel and a preparation method thereof.The preparation method comprises the following steps that anhydrous citric acid, choline chloride and water are mixed to react, and a ternary eutectic solvent is obtained; dispersing loofah sponge purified cellulose in a ternary eutectic solvent, washing and filtering a product to be neutral, dispersing the product in water, and performing ultrasonic treatment to obtain loofah sponge nanocellulose turbid liquid; dissolving sodium lignin sulfonate and lithium chloride in a sodium hydroxide solution to obtain a precursor solution; mixing the loofah sponge nanocellulose suspension with water, polyacrylamide, ammonium persulfate and N, N-methylene bisacrylamide, pouring the precursor solution into the loofah sponge nanocellulose suspension, uniformly stirring the mixture, pouring the mixture into a mold, and sealing the mold; and forming to obtain the loofah sponge nanocellulose hydrogel. The invention also provides an application of the hydrogel. The hydrogel disclosed by the invention has excellent biocompatibility, mechanical property and conductivity, has a wide application prospect in the field of flexible electronics, and is simple in preparation process, low in cost, green and environment-friendly.

Disclosed in the present invention are a biomass-derived graded porous asymmetric coordination monatomic carbon-based electrocatalyst, and a preparation method therefor and the use thereof. The preparation method for the biomass-derived graded porous asymmetric coordination monatomic carbon-based electrocatalyst comprises the following steps: (1) stirring and mixing de-alkalized lignin with a transition metal salt, a nitrogen-containing compound and a template, i.e., magnesium oxide, in a solvent until uniform, so as to obtain a suspension, wherein the transition metal comprises iron and zinc; (2) heating, evaporating and drying off the suspension, so as to obtain a lignin / metal ion / nitrogen-containing compound / template mixed precursor; and (3) subjecting the mixed precursor to high-temperature pyrolysis under the protection of an inert gas, cooling same, and then sequentially washing and drying same, so as to obtain a monatomic carbon-based electrocatalyst. The monatomic carbon-based electrocatalyst provided in the present invention has graded pores of micropores, mesopores and macropores, a surface nanosheet and an asymmetric coordination structure of Fe-N3O at the same time, and can be applied to fuel cells and metal-air cells.

The invention relates to laccase as well as a mutant and application thereof, and belongs to the technical field of biology. The amino acid sequence of the laccase PnLac1 is as shown in SEQ ID NO. 2, and the nucleotide sequence of the laccase PnLac1 is as shown in SEQ ID NO. 1. The mutant is characterized in that aspartic acid at the 166th position of laccase PnLac1 is subjected to site-specific mutagenesis, histidine at the 348th position of laccase PnLac1 is subjected to site-specific mutagenesis, methionine at the 403th position of laccase PnLac1 is subjected to site-specific mutagenesis, or leucine at the 467th position of laccase PnLac1 is subjected to site-specific mutagenesis, amino acid sequences are shown as SEQ ID NO.4, SEQ ID NO.6, SEQ ID NO.8 and SEQ ID NO.10, and nucleotide sequences are shown as SEQ ID NO.3, SEQ ID NO.5, SEQ ID NO.7 and SEQ ID NO.9. The decoloring efficiency of the laccase PnLac1 on crystal violet reaches 5h, and the degradation rate reaches 75%. When the ABTS is used as a substrate, the enzyme activities of the laccase mutants H348F and L467M are respectively improved by 1.89 times and 1.65 times, and the ABTS has the potential of degrading stubborn substances; when guaiacol is used as a substrate, the enzyme activities of the laccase mutants D166A and M403F are respectively improved by 2.07 times and 3.72 times, and the guaiacol has a relatively high application prospect in the field of lignin.

Provided in the present application are a bio-based fluoride-free waterproof agent, and a preparation method therefor and the use thereof. In the present application, firstly, a bio-based monomer is added into a reaction; secondly, a rosin-based amino acid surfactant is introduced, which is a bio-based ampholytic surfactant, has the advantages of good decontamination, resistance to hard water and being stable to an acid and an alkali and has the performances of sterilization, corrosion prevention, static electricity resistance, etc.; and finally, a lignin-furfural bio-based resin is added after the reaction, which resin is a new furfural bio-based resin, can be uniformly cross-linked on the surface layer of a yarn to adhere fiber ends to the yarn, and has a relatively strong adhesive force, such that the resin can bond the fibers, thereby limiting the movement of the fibers. The raw materials of the fluoride-free waterproof agent prepared in the present application are derived from bio-based products and are completely biodegradable in the natural environment, such that the fluoride-free waterproof agent is environmentally friendly and safe, thereby increasing the regenerative and environmentally friendly values of the product and having significant social benefits and wide market prospects.

The invention discloses high-specific-surface-area micro-mesoporous biomass porous carbon as well as a preparation method and application thereof. The method comprises the following steps: performing hydrothermal catalytic reaction on tobacco stems in an H2O2 solution to loosen a compact structure and increase oxygen-containing functional groups, fully mixing pretreated tobacco stem hydrothermal carbon and sodium lignin sulfonate in an aqueous solution in a solid-liquid form, adding glutaraldehyde to perform cross-linking reaction, and drying to obtain the tobacco stem hydrothermal carbon / sodium lignin sulfonate composite material. The preparation method comprises the following steps: preparing CaCO3 and tobacco stem into a composite biomass charcoal source with a stable three-dimensional network structure, preparing a composite biomass / CaCO3 precursor through an in-situ coprecipitation method, and finally carrying out high-temperature carbonization to obtain the tobacco stem / lignin composite porous carbon with a micro-mesoporous structure, a high specific surface area and a stable structure. The porous carbon material not only has abundant and mutually communicated hierarchical porous structures and high specific surface area, but also contains abundant active oxygen-containing functional groups, so that the surface characteristics of the porous carbon are improved, more adsorption sites can be provided, and the adsorption performance of the porous carbon material is greatly improved.

The invention relates to the technical field of building materials, and particularly discloses a slag and ardealite synergetic activated gypsum batten and a preparation method thereof.The slag and ardealite synergetic activated gypsum batten comprises pretreated ardealite, superfine slag powder, a composite exciting agent, reinforced fibers, self-repairing microcapsules, a foaming agent and water, and the composite exciting agent is formed by compounding nano SiO2, CaO and metakaolin; the preparation method comprises the following steps: mixing nano silica sol and a lignin solution, performing spray drying, and performing heat treatment to obtain the self-repairing microcapsule; the preparation method comprises the following steps: carrying out dry mixing on pretreated phosphogypsum, superfine slag powder and a composite exciting agent, adding water, adding reinforced fibers, self-repairing microcapsules and a foaming agent, stirring to form slurry, carrying out injection molding on the slurry, and carrying out stepped maintenance of pre-maintenance, main maintenance and carbonization treatment to prepare the modified red mud-pretreated phosphogypsum-based self-repairing self-repairing composite phosphogypsum-based self-repairing self-repairing composite phosphogypsum-based self-repairing composite phosphogypsum-based self-repairing composite phosphogypsum-based self-repairing composite phosphogypsum. The composite excitant synergistically activates the activity of the slag, and is matched with the reinforced fibers, the self-repairing microcapsules and stepped curing, so that solid waste resource utilization is realized, the compression resistance, bending resistance and impact resistance of the batten are improved, and the environment-friendly and high-performance effects are achieved.

The invention discloses a bionic lignin-based saline-alkali soil improvement material and a preparation method thereof, and belongs to the technical field of high polymer materials and soil improvement. According to the saline-alkali soil improving material, lignin or a derivative thereof serves as a framework, glutenin charge distribution and a dynamic disulfide bond sodium ion response function are simulated, and the sequence and the proportion of chain segments of sulfonic acid groups and quaternary ammonium salt groups are accurately regulated and controlled through step-by-step activation and alternate copolymerization in an aqueous solution polymerization reaction system; positive and negative charges form more uniform sequence distribution, so that the amphipathy of the lignin compound is realized; then, sulfydryl is introduced through click chemistry, linear regulation and control of material viscosity and water solubility are achieved through the chain transfer characteristic of multi-sulfydryl functional groups, and the requirements of different saline-alkali soil application scenes are met. The limitation that a traditional lignin product is single in function is broken through, efficient utilization of lignin is achieved, and the intelligent saline-alkali soil improvement material integrating high stability and pH and Na < + > multi-environment response is developed.

The invention discloses a lignin-monofluoroacrylic polymer high-voltage lithium ion binder as well as preparation and application thereof. The binder is prepared from lignin, polyfluoroacrylic acid and copolymer thereof through esterification reaction. The preparation method comprises the following steps: firstly, preparing a monofluoroacrylic polymer through free radical polymerization, and then grafting lignin into a side chain of the monofluoroacrylic polymer by utilizing esterification reaction to form a three-dimensional network structure; the binder has the free radical scavenging capacity of lignin, and the characteristics of chelating transition metal ions and stabilizing a CEI interface of polymonofluoroacrylic acid. Compared with a commercial PVDF (Polyvinylidene Fluoride) binder, the binder material disclosed by the invention has the advantages that the adhesive force is improved by 5.6 times, and the voltage resistance reaches 4.93 V which is obviously higher than that of PVDF (4.58 V). Therefore, the binder can be used for preparing the positive electrode of the high-voltage lithium ion battery, and has remarkable performance advantages.

The invention provides a lignin-based flame-retardant bacterial cellulose gel polymer electrolyte, preparation and application, the lignin-based flame-retardant bacterial cellulose gel polymer electrolyte is prepared by compounding a modified lignin material, a composite lithium salt and bacterial cellulose, when the polymer electrolyte is greater than or equal to 700 DEG C, the carbon residue rate is greater than or equal to 49.52%, the heat release rate peak value is less than or equal to 61.12 kW m <-2 >, and the flame-retardant bacterial cellulose gel polymer electrolyte has a good flame-retardant effect. The smoke release rate peak value is less than or equal to 0.0089 m < 2 > s <-1 >, and T5 is greater than or equal to 246.9 DEG C. Under the condition of room temperature, the ionic conductivity of the gel polymer electrolyte is 2.37-5mS cm <-1 >, the lithium ion transference number is greater than or equal to 0.75, and the electrochemical window is greater than or equal to 4.87 V (vs Li < + > / Li); in addition, when the temperature is less than or equal to-20 DEG C, the ionic conductivity is more than or equal to 0.162 mS cm <-1 >. The lignin-based flame-retardant bacterial cellulose gel polymer electrolyte not only has an excellent flame-retardant effect, but also has relatively good electrochemical performance.

The invention discloses a biodegradable polylactic acid composite material and a preparation method thereof, and relates to the technical field of polylactic acid materials. The method comprises the steps that firstly, chitosan activated through hydrochloric acid is subjected to graft modification through a KH550 silane coupling agent, meanwhile, nano-zinc oxide is subjected to surface modification through stearic acid, the chitosan and the nano-zinc oxide are mixed, spray drying is conducted, and antibacterial composite powder is prepared; the preparation method comprises the following steps: performing hydrochloric acid activation and KH570 silane coupling agent modification on nano silicon dioxide, performing hydroxypropylation modification on alkali lignin, and performing reflux crosslinking reaction on the modified alkali lignin, KH570 modified nano silicon dioxide, polycaprolactone, methyl methacrylate and azodiisobutyronitrile to prepare a heat-resistant reinforcer; uniformly mixing polylactic acid, poly (butylene succinate), the antibacterial composite powder, the heat-resistant reinforcer, tributyl citrate, polyethylene glycol and talcum powder, and extruding and granulating by using a twin-screw extruder to obtain the heat-resistant antibacterial material. The composite material prepared by the invention has excellent antibacterial property, heat resistance and biodegradability.

The invention relates to the technical field of sodium ion batteries, in particular to a preparation method and application of a closed-cell hard carbon material constructed based on a multi-benzene-ring plane structure. Comprising the following steps: (1) crosslinking lignin powder to obtain a macromolecular lignin precursor; (2) placing the macromolecular lignin precursor in the step (1) in an inert atmosphere furnace for first calcination to obtain hard carbon powder with a multi-benzene-ring plane porous structure; and (3) placing the hard carbon powder obtained in the step (2) in an inert atmosphere furnace for secondary calcination to obtain the closed-cell hard carbon material. According to the preparation method, lignin molecules are synergistically regulated through crosslinking-calcination to form a multi-benzene-ring plane structure, and then a high-closed-pore structure is constructed through graded calcination, so that the electrochemical performance of the lignin in the sodium-ion battery can be remarkably improved, the reversible capacity of 340 mAh / g and 500-circle stable circulation are obtained, and high-value utilization of the biomass material lignin is realized.

The present invention relates to the technical field of special gas purification, and specifically discloses a porous adsorption material for removing neon from helium and a preparation method thereof. The method comprises the following steps: (1) preparing a lignin raw material treated by fermentation; (2) preparing a carbon precursor; (3) placing the carbon precursor in a mixed solution containing a metal salt and a surfactant, stirring and impregnating to obtain a carbon precursor impregnated with the metal salt; (4) washing the carbon precursor impregnated with the metal salt to remove the excessively deposited metal salt, and drying to obtain a washed carbon precursor; (5) activating and creating pores in the washed carbon precursor with carbon dioxide at a high temperature to obtain a porous adsorption material for removing neon from helium. The porous carbon adsorption material prepared by the present invention using renewable natural biomass raw materials has a uniformly distributed pore structure and has a high adsorption and capture ability for neon under low-temperature conditions, and can effectively remove neon impurities in helium.

This disclosure relates to the fabrication of high-performance thin film composite (TFC) reverse osmosis (RO) membranes comprising a thin polyamide rejection layer (thickness of 100-200 nanometer), a porous substrate including polysulfone (PSf) layer (thickness of 40-50 micron) cast on polyester nonwoven fabric (thickness of ˜100 micron). Hydrophilic and antibacterial TFC polyamide RO membranes were developed by incorporating green Lignin and nanostructured silver-based metal organic frameworks (MOFs) into the selective layer. The polyamide layer of TFC RO membranes was fabricated on the porous PSf substrate by interfacial polymerization between aqueous monomer solutions containing MPD, and adequate additives in water and organic monomer solutions containing TMC in the mixture of hexane and co-solvents. The optimized produced RO membranes were provided water flux of 95-100 LMH and sodium chloride (NaCl) salt rejection of 98.5-99.0% during filtration of 2000 ppm NaCl solution at 225 psi pressure, and water flux of 55-60 LMH and sodium chloride (NaCl) salt rejection of 98.6-98.9% during filtration of 35000 ppm NaCl solution at 800 psi pressure. This disclosure also relates to developing a roll-to-roll PSf membrane as a substrate for making TFC RO membranes for water desalination

The invention relates to the technical field of collagen peptide extraction, and particularly discloses aquatic collagen tripeptide and a processing technology thereof, and the processing technology comprises the following steps: step S1, fish skin pretreatment; step S2, performing enzymolysis; step S3, fermentation; step S4, separation and purification; starting from three aspects, firstly, a modified degreasing agent is added in the degreasing treatment process, the modified degreasing agent takes 1-ethyl-3-methylimidazole acetate, zinc phytate and calcium citrate as main components according to a specific mass ratio, and secondly, gel emulsion is added in the enzymolysis process, so that the modified degreasing agent is prepared. The gel emulsion has an oil gel structure, grape seed oil and sodium lignin sulfonate, and 3, a composite strain is added in the fermentation process, the composite strain is prepared by mixing bacillus natto and lactobacillus plantarum according to a specific proportion, and the purity and the extraction rate of the aquatic collagen tripeptide are improved through the synergistic effect of all the procedures.

The invention provides a method for jointly evaluating the cellulose content and the lignin content of poplar wood based on a Pto-CDPK4 haplotype and application of the method, and belongs to the technical field of forest molecular breeding. According to the method disclosed by the invention, genotypes of mutation sites at the 248th site, the 255th site, the 297th site, the 363th site and the 367th site of a Pto-CDPK4 promoter region are detected, and according to a haplotype combination of the five sites, the cellulose and lignin contents of the poplar wood are accurately predicted and jointly evaluated; therefore, excellent individuals with high cellulose content and low lignin content in the wood can be accurately and efficiently screened in the early growth stage of the poplar, the breeding period can be remarkably shortened, and the breeding efficiency can be improved.

The invention provides a method for efficiently purifying lignin by using a composite alkali system, and relates to the technical field of lignin extraction and purification, wood fiber biomass is used as a raw material, KOH / thiourea composite alkali liquor with a specific mass ratio is adopted, and directional dissociation reaction is carried out under the conditions that the solid-to-liquid ratio is 1: (5-15), the temperature is 60-150 DEG C and inert gas protection is adopted; and after the reaction is ended, through a gradient acidification process (controlling the pH to be 10-12 and the pH to be 3-5 in stages) and by combining centrifugal filtration and water washing, ash content and carbohydrate residues are remarkably reduced, and high-quality lignin with the purity being greater than or equal to 91.16% is obtained. The method solves the problems of low purity and high impurity residue in the traditional process, and provides core raw material guarantee for lignin-based capacitor carbon.

The invention relates to the technical field of crop straw treatment and soil improvement, in particular to a rice straw returning method for improving soil fertility, which mainly comprises the following steps: crushing rice straws, soaking with a sodium hydroxide solution, and drying; then selecting specific strains, mixing and fermenting to prepare a microbial agent; preparing a composite additive in proportion; within 1-2 days after the rice is harvested, the pretreated straw, the microbial agent and the composite additive are applied to the field, deep ploughing is conducted, and irrigation is conducted; and finally, performing rotary tillage and soil preparation after straw decomposition, and monitoring and adjusting the pH value of the soil when next-stubble crops are planted. According to the method, straw is pretreated, lignin is destroyed through a sodium hydroxide solution, the particle size is accurately controlled, drying is conducted in cooperation, the microbial decomposition efficiency is improved, straw and soil are fully mixed through accurate ploughing in the field, decomposition is accelerated into nutrients, the straw can be powerfully decomposed through a microbial agent, and the soil micro-ecology is improved; the composite additive supplements various nutrients and enhances the water and fertilizer retention capability of soil.

The invention relates to the technical field of gypsum fiberboard raw material treatment, in particular to a plant fiber for a gypsum fiberboard and a preparation method and application thereof. Compared with the prior art, the preparation method provided by the invention adopts specific process steps, plant fiber raw materials are softened by using a supersaturated calcium hydroxide solution to partially remove lignin, the treated plant fiber slurry is introduced into a sulfuric acid solution for neutralization in the water washing process, and generated nano-scale calcium sulfate particles are attached to the surfaces of the plant fibers, so that the surface of the plant fibers is uniform. The plant fiber coagulant plays a role of a coagulant during production of the gypsum fiberboard, and the plant fiber meeting the requirements of production of the gypsum fiberboard is prepared. The preparation method provided by the invention is low in cost, and the gypsum fiberboard prepared from the obtained plant fiber for the gypsum fiberboard has relatively high mechanical property indexes and meets the performance requirements of boards for building decoration.