90 results about "Lignan" patented technology

The invention belongs to the technical field of pharmaceutical preparations, and relates to traditional Chinese medicine self-assembled nanoparticles for enhancing anti-inflammatory activity as well as a preparation method and application of the traditional Chinese medicine self-assembled nanoparticles. The traditional Chinese medicine self-assembled nanoparticles are prepared from biphenyl cyclooctene type lignan and an oleanane type pentacyclic triterpenoid compound, wherein the mass ratio of the biphenyl cyclooctene type lignan to the oleanane type pentacyclic triterpenoid compound is 1 to (0.125 to 8). Through an anti-solvent exchange method, the carrier-free nanoparticles based on self-assembly of the traditional Chinese medicine active ingredients are prepared by utilizing spontaneous non-covalent interaction between the biphenyl cyclooctene lignan and the oleanane pentacyclic triterpenoid, so that the cytotoxicity is remarkably reduced, the biocompatibility is enhanced, and the toxic and side effects are reduced; meanwhile, the traditional Chinese medicine self-assembled nano-particles have a remarkable cellular uptake effect, the absorption and utilization efficiency of the medicine is improved, and more efficient anti-inflammatory biological activity is shown.

The invention relates to the technical field of cosmetic functional raw material preparation methods, in particular to a plant extract and an extraction method and application thereof. The invention provides a plant extraction method, which comprises the following steps: adding a glycol ether or polyhydric alcohol solvent containing 5-7 carbon atoms into a product containing phenolic acid compound components and lignan compound components, carrying out homogeneous reaction, carrying out heating extraction, and collecting the extract. By adopting a supramolecular technology, phenolic acid compound components can form a supramolecular solvent with glycol ether or polyol solvents containing 5-7 carbon atoms, and the supramolecular solvent is further combined with lignan compound components to form a supramolecular structure, so that the stability of an extracting solution is remarkably enhanced, the specific enrichment of the lignan compound components is realized, and the specific enrichment of the lignan compound components is realized. The problems that an existing extraction technology is poor in stability, poor in formula applicability, not prominent in effect and the like are successfully solved.

The invention provides lignan, and belongs to the technical field of medicines. According to the invention, a compound shown as a formula I and a compound shown as a formula II are extracted, separated and identified from schisandra chinensis oil for the first time, and the compound shown as the formula I and the compound shown as the formula II are superior to a positive drug group arbutin in the aspect of inhibiting generation of melanin of mouse skin melanoma cells (B16F10); an inflammatory factor release inhibition experiment research shows that the compound shown in the formula I and the compound shown in the formula II have relatively good activity in the aspect of inhibiting LPS-induced mouse mononuclear macrophage RAW264.7 from releasing IL-6 and TNF-alpha; in a skin irritation experiment, the schisandra chinensis non-saponifiable matter containing the compound shown in the formula I and the compound shown in the formula II is non-irritant to the skin of a white rabbit, and it is proved that the compound shown in the formula I and the compound shown in the formula II can be applied to preparation of whitening and anti-inflammatory products. Formula I; formula II.

This invention discloses a novel chalcone gypsum glycoside compound with the structure shown in Formula I. The chalcone gypsum glycoside compound of this invention has a novel and unique structure, and for the first time, it is disclosed that this compound possesses significant hepatoprotective activity and can be used in the preparation of hepatoprotective drugs. This invention also discloses the use of the aforementioned novel chalcone gypsum glycoside compound in the preparation of hepatoprotective drugs.

The invention relates to the technical field of pharmacy, in particular to a dibenzyl butane type lignan LCA analogue as well as a preparation method and application thereof. Based on the structural characteristics of a JAK1 inhibitor, LCA is taken as a skeleton, druggability optimization and a direct drug design and structure simplification strategy based on a receptor structure are comprehensively utilized, a nitrogen-containing heterocyclic ring and a nitrogen-containing functional group are introduced into a side chain, systematic structural modification and simplification are carried out, and a series of novel LCA derivatives are designed and synthesized. The invention aims to obtain an anti-RA JAK1 inhibition candidate compound with higher activity, higher selectivity and potential.

The invention discloses a tetrahydrofuran lignan compound as well as a preparation method and application thereof, the tetrahydrofuran lignan compound is a compound shown as a formula (I) or pharmaceutically acceptable salt thereof, the compound is a natural compound extracted from rubus corchorifolius stems, has anti-inflammatory activity and anti-tumor activity, and can be used for preparing a medicine for treating tumors. The compound has the potential of being developed into anti-inflammatory or anti-tumor drugs and can be used as a lead compound.

The application discloses a method for simultaneously producing five components in flaxseed, comprising the following steps: (1) preliminarily extracting flaxseed gum and lignan from flaxseed; (2) preparing flaxseed oil by cold pressing of degummed flaxseed through a hydraulic oil press; (3) performing skin kernel separation on the degummed and defatted flaxseed; (4) crushing the flaxseed skin after the skin kernel separation to extract flaxseed gum and flax lignan again; (5) preparing flaxseed oil again from the flaxseed kernel after the skin kernel separation; (6) extracting flaxseed protein from the defatted flaxseed kernel; (7) separating flax cyclic peptide from the flaxseed oil prepared twice; and (8) extracting flax cyclic peptide again from the defatted and deproteinized flaxseed kernel. The method is interlocked, the preliminary extraction or preparation of partial components provides favorable conditions for the flaxseed skin kernel separation, and after the flaxseed skin kernel separation, the extraction or preparation rate of the components and other components is improved, the components are complementary, the method has low cost, simple and easy-to-operate process, is green and environment-friendly, can be produced on a large scale, and in addition, the five components are simultaneously produced, so that the added value of the flaxseed is greatly improved.

This invention provides a high-performance liquid chromatography (HPLC) method for the detection of magnolamine, lignans, and asaran in *Zanthoxylum bungeanum* slices. The chromatographic conditions are as follows: a CAPCELL PAK C18 column (250 mm × 4.6 mm, 5 μm); mobile phase A: 0.3% phosphoric acid solution; mobile phase B: acetonitrile; detection wavelengths: magnolamine 217 nm, lignans: 201 nm, asaran: 201 nm; column temperature: 25–35 °C; mobile phase flow rate: 0.5–0.8 mL / min; gradient elution. This method achieves complete separation of magnolamine, lignans, and asaran, enabling the detection of their contents in *Zanthoxylum bungeanum* slices. It features high sensitivity, strong specificity, simple experimental operation, and the ability to simultaneously determine both alkaloids and lignans, allowing for more comprehensive quality control of *Zanthoxylum bungeanum* slices.

A method and formulation for improvement in at least the texture and shelf stability of starch-based foods is disclosed, such as but not limited to gummy candies, through the incorporation of polyphenols, wherein said polyphenols may include but are not limited to one or more of flavonoids, phenolic acids, lignans, stilbenes, and tannins. This method and formulation comprises the use of polyphenol molecules to improve and stabilize starch-based foods for extended periods of time without the use of artificial preservatives. The method and formulation including one or more polyphenols also allows utilization of specific beneficial qualities of the polyphenols including: flavor, aroma, color and the numerous associated health benefits. Thus, the method and formulation facilitates the production of various starch-based foods (e.g., chewy candies, mochi, breads, cakes, pastas, etc.) with unique and previously unattainable qualities that are advantageous for consumer-packaged foods.

The application provides a luffa bath liquid with antibacterial and anti-inflammatory functions, which comprises the following components by weight: 10-18 parts of a ketone compound extract as a first component, 22-35 parts of a lignan compound extract as a second component, 2-5 parts of a triterpene compound as a third component, and 20-30 parts of butyl avocadoate as a fourth component. The luffa bath liquid has an effect of inhibiting the respiratory burst of neutrophils to a certain extent, and can prevent the occurrence of allergic diseases and some cardiovascular and cerebrovascular diseases. The effect is obtained by combining the first component, the second component and the third component.

The invention discloses a preparation method of high-activity flaxseed powder, which comprises the following steps: (1) detoxification treatment: mixing flaxseeds and water according to a mass ratio of 1: 4-1: 6, carrying out microwave treatment, washing with water, and drying; (2) pulping treatment: mixing and pulping the detoxified flaxseeds and water according to a mass ratio of 1: 3.5-1: 4.5, adjusting the solid content of the pulp to 12-14%, and then carrying out enzymolysis treatment; (3) emulsification treatment: adding soybean lecithin into the enzymolysis product, and shearing and emulsifying to obtain an emulsion; (4) homogenizing treatment: adopting two-stage homogenizing; and (5) performing spray drying to obtain the high-activity flaxseed powder. According to the preparation method disclosed by the invention, multiple steps of microwave detoxification, enzymolysis treatment, composite emulsification, secondary homogenization and the like are cooperated, so that cyanogenic glycoside is efficiently removed, meanwhile, the retention rate of alpha-linolenic acid and lignan is remarkably increased, and the product is endowed with excellent solubility, emulsification stability and brewing performance.

The invention discloses novel lignan in Chinese starjasmine stem as well as an extraction method and application of the novel lignan. The novel lignan extract in the Chinese starjasmine stem is obtained by crushing the Chinese starjasmine stem, soaking the Chinese starjasmine stem in methanol, carrying out vacuum concentration, carrying out macroporous resin adsorption, eluting, carrying out Sephadex LH-20 gel column chromatography, carrying out silica gel column chromatography and carrying out ChiralCore Cel-J chromatographic column separation. The lignan compound can inhibit the expression of IL-1beta and IL-6 in RAW 264.7 cells induced by lipopolysaccharide (LPS), and shows potential anti-inflammatory activity. The extraction method is simple, stable, reliable, convenient to operate and low in production cost, the novel lignan compound with the unique chemical structure can be extracted, and the novel lignan compound is high in purity, small in dosage of harmful reagents, low in toxicity and suitable for industrial production.

The present invention provides for a method to deconstruct a biomass: the method comprising: (a) introducing a solvent comprising a polyamine, or a mixture of polyamines, to a biomass to dissolve at least part of solid biomass in the solvent, wherein the polyamine is a Brønsted or Lewis base, and / or the polyamine is a hydrogen bond donor and / or acceptor; (b) optionally introducing an enzyme and / or a microbe to the solubilized biomass mixture such that the enzyme and / or microbe produces a sugar from the solubilized biomass mixture; (c) optionally separating the sugar from the solubilized biomass mixture; and (d) optionally separating the lignan from the solubilized biomass mixture.

The invention discloses flaxseed and wheat oligopeptide mixed powder with an effect of improving sarcopenia, and belongs to the field of biological medicines. The invention relates to a composition with an effect of improving sarcopenia. The composition comprises active ingredients including wheat oligopeptide and flaxseed meal, wherein the flaxseed powder contains secoisolariciresinol diglucoside, and a mixture of small molecules with the molecular weight smaller than 1000 Da in the wheat oligopeptide accounts for 90% or above. The invention discloses a differentiated molecular path on which the two components play roles, the wheat oligopeptide has outstanding effects in the aspects of remarkably activating a PI3K / Akt pathway and promoting FOXO3a phosphorylation inactivation, the mechanism complementarity of'upstream regulation 'and'downstream blocking' lays a solid theoretical foundation for realizing a more comprehensive anti-atrophy effect through combined use, and the wheat oligopeptide has a good application prospect. And a composite scheme with a fuzzy mechanism in the prior art is exceeded. In addition, the formula disclosed by the invention can also improve the in-vivo environment which is not beneficial to muscle maintenance from the source by relieving chronic inflammation and oxidative stress.

The application discloses a composition containing sesame lignan compounds and application thereof, and selects at least one of sesame lignan or similar compounds, glycoside compounds as a main active ingredient, so that the obtained composition can significantly improve physiological activities in the aspects of controlling fat in vivo, regulating intestinal flora, maintaining blood fat, blood sugar, blood pressure, protecting liver, controlling appetite, antioxidation and anti-aging, etc. Compared with single components, two or more than two compound combinations have a better synergistic effect. Meanwhile, the components in the composition are pure natural plant food raw materials, and have no toxic side effects. Therefore, the composition has a good application prospect.

The invention discloses an application of lignan B in preparation of a medicine for preventing and / or treating neurodegenerative diseases or neurological diseases. In-vitro cell experiments and animal model researches find that the Licarin B can significantly reduce the expression level of ABAD or inhibit the enzymatic activity of ABAD, so that the nerve cell activity is improved, neuroinflammation is reduced, the epileptic seizure frequency is reduced, and the cognitive function is improved. The invention provides the new application of the Licarin B in preparing the medicine for preventing or treating the neurodegenerative diseases and the epilepsy for the first time, and a new way and means are developed for preventing and treating the neurodegenerative diseases and the epilepsy.

The invention provides a lignan compound in phyllanthus emblica and application of the lignan compound in preparation of cosmetics and medicines, and belongs to the technical field of medicines. Five lignan compounds Phyllanthus emblica A-E, namely phyllanthus emblica A-E, are obtained from Phyllanthus emblica L. leaves, a thin layer chromatography method is mainly adopted for guidance, a high performance liquid chromatography separation technology and other plant chemical research means are combined, and a DPPH free radical scavenging activity evaluation model and an LPS-induced NO inhibition model are adopted, so that the phyllanthus emblica A-E can be used for preparing the phyllanthus emblica L. extract, and the phyllanthus emblica L. extract can be used for preparing the phyllanthus emblica L. extract, the phyllanthus emblica L. extract, the phyllanthus emblica L. extract and the phyllanthus emblica L. extract and the phyllanthus emblica L. extract. The traditional Chinese medicine composition is prepared into a solid, liquid or paste dosage form according to a conventional method, so that the anti-oxidation and anti-inflammatory treatment effects are realized. The lignan compound disclosed by the invention is simple and convenient in preparation method, high in yield and excellent in purity, and the obtained compound is a new natural secondary metabolite and has remarkable antioxidant and anti-inflammatory activity.

The application discloses strigasiaticaside A and an extraction method and application thereof, and finds, through chemical component research on the whole grass of striga asiatica, a new synbinaphthyl tetrahydrofuran lignan glycoside, that is, strigasiaticaside A, identifies the chemical structure of the new compound through nuclear magnetic resonance, mass spectrometry, ultraviolet, infrared and circular dichroism spectrum technology.The structure of the new compound is identified as (-) sesamin-5-O-beta-D-glucopyranoside.The anti-hepatitis activity of the new natural product is evaluated by using LPS stimulated HepG2 cells.The results show that when the concentration is 100 muM, the compound has a significant inhibitory effect on the production of inflammatory factors IL-10 and NF-kappa B protein, and has no cytotoxicity, indicating that the compound may have potential anti-hepatitis activity.

This invention discloses lignan compounds containing tannin structures, as shown in Formula I, isolated from Phyllanthus emblica. The inventors used an automated biochemical analyzer and the IFCC method to determine the AST and ALT activities in the serum of mice with a CCl4-induced liver injury model, finding that both AST and ALT were significantly reduced, indicating that the compound possesses hepatoprotective activity. This invention also discloses the use of the aforementioned lignan compounds containing tannin structures in the preparation of hepatoprotective drugs.

The invention relates to the technical field of preparation methods of cosmetic functional raw materials, in particular to application of a fructus arctii extraction product. The invention provides application of a fructus arctii extraction product in preparation of a product with the effect of improving the fiber structure order of a skin corium layer. According to the invention, a unique preparation process is adopted, and the active ingredients of phenolic acid and lignan substances are enriched, so that the expression of Fibulin-5, LOX, LOXL1 / 2 / 3, BMP1 and ADAMTS2 can be remarkably up-regulated by the fructus arctii extraction product, the ordered crosslinking of collagenous fibers is promoted, the activity of MMP-1 and MMP-12 is inhibited, the content of III-type collagen and elastin is increased, a multi-target and co-synergistic action mechanism is realized, and the effect of the fructus arctii extraction product is enhanced. Therefore, the potential of the fructus arctii extracted product for promoting ordered arrangement of fibers is improved, and reconstruction and reinforcement of a corium layer structure are realized on the molecular level.

The invention discloses a capsule preparation for supporting mood health and improving depression and hormone balance, and relates to the technical field of health food. The capsule preparation comprises the following active components and auxiliary materials in parts by weight: the active components: 3-6 parts of a Malaysia flaxseed extract (containing 12% of lignan), 2-5 parts of Singago pumpkin seed concentrated powder (containing 4mg / g of zinc), 1-3 parts of Indonesia sesame essence (containing lignan), 2-4 parts of a sunflower seed extract (containing vitamin E), 1-3 parts of an olive leaf extract (containing 0.5% of an olive split iridoid compound) and an angelica sinensis extract (containing 0.5% of an olive split iridoid compound). 2-5 parts of Hypericum perforatum (flavonoid gt; 2.5%) 3-7 parts. Through scientific and reasonable formula design, various active components with synergistic effects are organically combined, the dual effects of emotion regulation and hormone balance can be achieved from multiple targets and multiple ways, the content of effective components is stable, bioavailability is high, taking is convenient, safety is high, and the actual requirements of consumers can be met.

Disclosed in the present invention are an anti-SARS-CoV-2 arylnaphthalene lignan compound, and a preparation method therefor and the use thereof. Results show that ANL-1, ANL-2, and ANL-4 to ANL-7 have no toxicity to Vero E6 cells, and ANL-3 has low toxicity to Vero E6 cells. In a Vero E6 cell model, the in vitro anti-SARS-CoV-2 activities of ANL-7 and ANL-2, in TI value, are respectively 19 times and 16 times that of remdesivir, and are higher than the in-vitro anti-SARS-CoV-2 activity of a market-available drug. ANL-2 has a significant inhibitory effect on RNA-dependent RdRp activity of SARS-CoV-2, and ANL-2 also effectively limits the replication of various pathogenic coronaviruses in Caco2 cells or hamsters. It can be seen that the arylnaphthalene lignan compound has a good anti-coronavirus activity and can be used for treating and preventing SARS-CoV-2 infection or delaying the progress of SARS-CoV-2 infection in a patient.

The present application relates to a preparation of a 7,7'-epoxy lignan compound and its application in preparing anti-malaria products, and belongs to the field of traditional Chinese medicine and natural medicine pharmacy. The anti-malaria medicine with the compound as an active ingredient. The present application expands the medicinal value of 7,7'-epoxy lignan compounds in Rhodiola henryi.

The invention relates to the technical field of analytical chemistry detection, in particular to a color developing agent for rapidly measuring the content of lignan and a preparation method thereof.The color developing agent is composed of a liquid component and a solid component dispersed in the liquid component, and the liquid component comprises the following components by volume: a color developing agent, a 2, 4-dihydroxy-2, 4-dihydroxy-2, 4-dihydroxy-2, 4-dihydroxy-2, 4-dihydroxy-2, 4-dihydroxy-2, 4-dihydroxy-2, 4-dihydroxy-2, 4- adding an acidic ethanol solution of 2, 4-dinitrophenylhydrazine; the nano signal enhancer is surface modified gold nanorod sol with the concentration of 3 to 8 nM; the catalytic oxidant is iron (III) porphyrin with the concentration of 0.05 to 0.15 mmol / L; a dispersion stabilizer: polyethylene glycol 400 with a volume fraction of 1-3%; the solvent system is a mixed solvent of a buffer solution with the pH value of 2.0-3.0 and ethanol; the solid component is lignan molecularly imprinted polymer microspheres, and the concentration of the lignan molecularly imprinted polymer microspheres in the color developing agent is 0.5-2.0 g / L. According to the method, lignans can be conveniently detected, the sensitivity is high, the molecularly imprinted polymer provides specific recognition capability, and rapid, high-sensitivity and high-selectivity colorimetric detection on lignan components is realized.

Disclosed in the present disclosure is a method for extracting and separating various components from flaxseed meal based on a subcritical composite solvent, including the following steps: adding an extraction agent to flaxseed meal for subcritical extraction to obtain a crude flax lignan extract and crude flaxseed oil in one step, where the extraction agent includes n-butane and anhydrous ethanol at a volume ratio of 1:1; adding n-hexane to the crude flaxseed oil for fractional extraction, and conducting rotary evaporation to obtain flaxseed oil; and subjecting the flaxseed meal used to hot water extraction to obtain flaxseed gum. In the present disclosure, the subcritical composite solvent used in an extraction process can replace a large number of organic reagents used in a traditional extraction process, and has the characteristics of good solvent effect, environmental friendliness, recyclability, and low burden on the environment.

The application provides a surgical auxiliary instrument material with bacteriostatic performance and a preparation method thereof, relates to the technical field of bacteriostatic medical instruments, and comprises the following steps: heating and mixing polyethylene, polycaprolactone, styrene-butadiene block copolymer and a bacteriostatic precursor to obtain a mixture of the surgical auxiliary instrument material with bacteriostatic performance, and the raw materials of the bacteriostatic precursor include flax lignan, 4-vinyl-2,6-dichlorophenol and imino methacrylate. In view of the problem of poor wear resistance of the bacteriostatic surgical auxiliary instrument, the bacteriostatic precursor with high polymer coating flax lignan is prepared, the compatibility of flax lignan and the remaining high molecular materials (polyethylene, polylactic acid, styrene-butadiene block copolymer) is improved, the interface bonding performance is improved, the synergistic effect between flax lignan and other materials is exerted, the antibacterial substance is no longer a heterogeneous phase particle in the bacteriostatic surgical instrument, and the continuity, wear resistance and oxidation resistance of the whole material are maintained.

The invention discloses a lignan compound of which the structure is selected from one of the following structures: the lignan compound prepared by the invention has a remarkable inhibition effect on the activity of butyrylcholine esterase, and the binding free energy of the two lignan compounds and BChE is analyzed by performing molecular docking on the two lignan compounds and the butyrylcholine esterase. A molecular docking result proves a butyrylcholine esterase activity in-vitro inhibition experiment result, so that the lignan compound prepared by the invention has huge potential as a butyrylcholine esterase inhibitor.

The invention relates to the field of medicinal plant genetic engineering, and particularly discloses a CYP450 gene participating in lignan synthesis in a plant and a coding product thereof. The invention aims to analyze the oxidation modification step of the lignan biosynthetic pathway in the plant, and provides a gene element for the subsequent synthetic biological application. The invention discloses a C-5 hydroxylase gene sequence and an amino acid sequence thereof required by biosynthesis of aryl tetrahydronaphthalene lignans in podophyllum hexandrum for the first time. Reference is provided for subsequent rational design and directional transformation of lignan compound structure modification enzymes, development and utilization of biosynthetic pathway elements and synthetic biology heterologous efficient production of podophyllotoxin compounds.

The invention belongs to the field of biological medicine and analytical chemistry, and provides an LC-MS database of kadsura characteristic lignans and triterpenes as well as a construction method and application of the LC-MS database. The construction method comprises the following steps: collecting mass spectrum data of a sample with a reference substance, carrying out deconvolution, peak alignment and other treatment, matching and associating the molecular formula, molecular weight and other information of the reference substance with a secondary mass spectrum, and constructing a first database; the method comprises the following steps: converting a chemical structure of a schizandriaceae sample without a reference substance into a simplified molecular linear input specification format, calculating a molecular descriptor by utilizing an R language, analyzing and predicting retention time and a mass spectrum splitting decomposition mode through a preset prediction model, and constructing a second database. According to the application, actually measured data and a prediction model are integrated through double databases, so that the accuracy and comprehensiveness of kadsura ingredient identification are improved, the problem of isomeride identification and the problem of reference substance dependence are solved, a standardized analysis process is established, and key technical support is provided for quality control of medicinal materials, metabonomics research and modernization of traditional Chinese medicines.

The invention provides a lignan extraction method and a liquid chromatography detection method, and relates to the technical field of plant active ingredient extraction and detection.According to the method, flaxseeds are smashed, seed coats are separated, the enrichment degree of lignan is improved, fatty acid interference is effectively removed through soaking of n-hexane, and in addition, the content of lignan is increased. According to the invention, through the drying of the vacuum drying oven, the volatilization speed of n-hexane is greatly accelerated by utilizing a negative pressure environment, and the drying time is shortened from several hours in the prior art to about 30-60 minutes; alkali hydrolysis pretreatment is replaced with methanol ultrasonic extraction, a 70% methanol solution is used for ultrasonic extraction at the temperature of 40 DEG C, vortex mixing is combined, and the extraction efficiency is improved. In the supernate treatment process, the recovery rate of lignan is increased by repeatedly extracting for three times and combining the supernate. Finally, the pH is adjusted to 4-5 by accurately controlling the adding amount of hydrochloric acid, and the influence of excessive acidification on the stability of the sample is avoided.