134 results about "Amylose" patented technology

The invention belongs to the technical field of detection methods, and particularly relates to a liquid chromatography detection method for an idedecalciferol intermediate and impurities thereof. According to the liquid chromatography detection method for the eldecalciferol intermediate and the impurities thereof, a liquid chromatography method is combined with an electrospray detector for detection; chromatographic conditions are as follows: an amylose-tri (3-chlorphenyl carbamate) covalently bonded silica gel chromatographic column is adopted; and carrying out gradient elution by taking water as a mobile phase A and acetonitrile as a mobile phase B. The method for detecting the eldecalciferol intermediate and the impurities of the eldecalciferol intermediate is good in specificity, high in sensitivity and accuracy, good in repeatability and stability and high in analysis speed, the content of the eldecalciferol intermediate and the impurities of the eldecalciferol intermediate can be rapidly and accurately detected, and the blank of detection of the eldecalciferol intermediate and the impurities of the eldecalciferol intermediate in the prior art is filled.

The invention discloses a method for reducing cyanide in the grain moistening process of Maotai-flavor liquor, and belongs to the technical field of liquor brewing. The brewing raw material sorghum is subjected to gamma-ray irradiation pretreatment, and an abscisic acid solution is applied for multiple times in the grain moistening step in the brewing process. According to the method, 60Co-gamma ray irradiation and abscisic acid solution treatment are combined, the content of cyanogenic glycoside in baijiu brewing is controlled from the source, the content of cyanogenic glycoside in baijiu is remarkably reduced on the premise of not changing indexes such as moisture, crude protein, crude fat, total ash content, amylose and reducing sugar related to the quality of sorghum, and the quality of the baijiu is improved. And 105221.56 [mu] g / kg of a blank control group is reduced to 1395.32 + / -164.29 [mu] g / kg.

The application discloses a kind of umami extract, belong to food field.The preparation method of the umami extract with clam meat as raw material, after fermentation by specific strain, then the fermentation liquor is subjected to enzymolysis by specific composition of composite enzyme, filtration, irradiation treatment and freeze-drying is prepared.The step of fermentation before enzymolysis in the present application, through the synergistic effect of microorganism and composite enzyme can effectively avoid the generation of bitter taste, fishy smell and other undesirable flavors in extract, and the free amino acid content of the umami extract prepared is significantly increased, and the percentage of glutamic acid, aspartic acid and other umami amino acids in total free amino acid is also significantly improved.In addition, the present application also proves by experiment that adding amylose, sugar cane molasses and bonito powder in clam meat slurry during fermentation can further increase the content of free amino acid and the percentage of umami amino acid in umami extract, so that the flavor of the umami extract prepared is better.

The invention belongs to the field of plant engineering technology and crop germplasm resource innovation, and particularly relates to a rice BEIIb gene mutant, application of the rice BEIIb gene mutant in improving rice resistant starch and an improving method. A mutant material with high resistant starch content is created by knocking out the rice BEIIb gene, and compared with a wild type, mutant seeds show an obvious chalky phenotype, composite starch particles are abnormal in a spherical or ellipsoidal shape, the amylose content is remarkably increased, the protein content is remarkably reduced, the resistant starch content is remarkably increased, and the resistant starch content is remarkably reduced. The hardness of cooked rice is remarkably increased, and the elasticity is remarkably reduced. Through transcriptomics analysis, the protein mainly participates in biological pathways such as carbohydrate metabolism, chloroplast photosynthesis, calmodulin combination and secondary metabolite synthesis, and the expression level of endosperm starch synthesis related genes is obviously changed. The method can provide theoretical basis and method reference for breeding of high-quality rice varieties with high resistant starch.

The invention relates to a method for analyzing and detecting a non-azonetin enantiomer, which adopts normal-phase high performance liquid chromatography and takes normal hexane-ethanol-trifluoroacetic acid as a mobile phase to detect the non-azonetin enantiomer, and the specific chromatographic conditions are as follows: a chromatographic column is prepared by covalently bonding amylose-tri (3, 4, 6-trimethyl-1, 3-pentanediol) on the surface of silica gel; 2, 5-dichlorophenyl carbamate is used as a filling agent (IE, 4.6 * 250 mm, 5.0 [mu] m); a mobile phase: n-hexane: ethanol: trifluoroacetic acid = 500: 500: 1 (V / V / V); the flow rate is 1.0 ml / min; the column temperature is 30 DEG C; the sample size is 20 [mu] l; the detection wavelength is 280 nm; the elution gradient is isocratic elution. The invention provides a high performance liquid chromatography method for analyzing the enantiomer of the fiazonetin, the complete separation of the fiazonetin and the enantiomer of the fiazonetin can be realized by adopting the method, and the separation degree of a main component and the enantiomer is greater than 1.5.

An emulsified composition containing methyl cellulose, a starch material, edible oil or fat, and an emulsifying material containing protein. The starch material is a component (A) which is a powdery material satisfying (1) a starch content is 75% by mass or more, (2) 3-45% by mass of a low molecular weight starch (a peak molecular weight of equal to or more than 3×103 and equal to or less than 5×104) including 5% by mass or more of an amylose content, (3) a degree of swelling in cold water at 25° C. is 5-20 and (4) a content under a sieve with openings of 3.35 mm and on a sieve with openings of 0.038 mm is 60-100% by mass; and / or a component (B) a raw material starch of which is a cornstarch, a tapioca starch, a wheat starch, and / or a pea starch.

The invention discloses a preparation method of zein-starch Pickering emulsion loaded with daidzein and relates to the technical field of emulsion preparation, waxy corn starch is subjected to enzymolysis through pullulanase and is applied to emulsion construction, and the emulsion with good stability is researched to realize efficient loading. The invention also discloses a zein-short amylose composite nanoparticle, a preparation method of a Pickering emulsion stabilized by the composite nanoparticle, and the Pickering emulsion stabilized by the composite nanoparticle. The Pickering emulsion with stable composite nanoparticles prepared by the invention has the characteristics of high stability and high safety, and provides a substitute with a good application prospect for the transportation of a bioactive substance daidzein.

This invention relates to a gene qAC that regulates amylose content in rice and a method for improving amylose content using the qAC gene. The qAC gene includes qAC6.1, qAC6.2, and qAC9.1 genes. The nucleotide sequence of the coding region of the qAC6.1 gene is shown in SEQ ID NO.1, the nucleotide sequence of the coding region of the qAC6.2 gene is shown in SEQ ID NO.3, and the nucleotide sequence of the coding region of the qAC9.1 gene is shown in SEQ ID NO.5. The amino acid sequence of the coding region of the qAC6.1 gene is shown in SEQ ID NO.2, the amino acid sequence of the coding region of the qAC6.2 gene is shown in SEQ ID NO.4, and the amino acid sequence of the coding region of the qAC9.1 gene is shown in SEQ ID NO.6. Through this invention, the rice qAC gene is highly expressed in seeds, specifically improving the amylose content of rice grains without significantly affecting other major agronomic traits of the rice plant. Knocking out the qAC gene in rice using genetic engineering technology can significantly reduce the amylose content of rice and improve the cooking and eating quality of rice. Therefore, the qAC gene has a promising application prospect in the breeding of high-quality rice.

The invention belongs to the field of molecular biology, and discloses application of ZmUUP5 protein in regulation and control of corn kernel quality and yield traits. The amino acid sequence of the ZmUUP5 protein is as shown in SEQ ID NO. 2, and the nucleotide sequence of the ZmUUP5 protein coding gene is as shown in SEQ ID NO. 1. It is found that the ZmUUP5 protein can regulate and control the corn kernel quality and yield traits, mutation of the ZmUUP5 gene in corn can improve the protein content, overexpression of the ZmUUP5 gene can cause grain enlargement and hundred-grain weight increase, the ZmUUP5 protein can be used for improving the corn yield, and the content of amino acid and amylose in grains can also be improved. The discovery not only can provide a scientific basis for analyzing the development mechanism of grains, but also can provide theoretical guidance for genetic improvement of corn.

The present invention provides glutinous wheat flour that gives noodles with moderate firmness and stickiness, and a good texture with a good bite, a noodle mix containing this glutinous wheat flour, and a method for producing this glutinous wheat flour. [Solution] The glutinous wheat flour, including wheat flour obtained from wheat with a triple deletion of the amylose synthesis gene, has an average particle size of 50 μm or less and a maltose value of 200 mg / 10 g or less. The glutinous wheat flour content in the noodle mix is ​​in the range of 10% by mass or more and 50% by mass. The method for producing glutinous wheat flour includes a mixing step in which wheat flour obtained from wheat with a triple deletion of the amylose synthesis gene and other wheat flours are mixed, and the average particle size of this glutinous wheat flour is 50 μm or less and the maltose value is 200 mg / 10 g or less.

The invention provides whole-grain matrix powder of a special dietary food for diabetics and a preparation method of the whole-grain matrix powder, and belongs to the technical field of food processing. Comprising the following steps: adding beans into grains, performing crushing, adding pullulanase and cellulase, performing mixed enzymolysis, after the mixed enzymolysis is completed, adding high-temperature alpha-amylase, performing enzymolysis to obtain standby powder B, performing extrusion puffing, and performing crushing to obtain the whole-grain matrix powder. According to the invention, a certain amount of beans are added into grains, so that reducing sugar and protein are subjected to Maillard reaction, and dextrin, fatty acid and protein are subjected to chemical reaction, so that the flavor and reconstituability of the whole-grain matrix powder are ensured. According to the invention, a multi-enzymolysis combined extrusion puffing process is adopted, so that the contents of amylose and resistant starch in the whole grain matrix powder are increased, and the blood sugar stability of diabetic patients is favorably maintained. Meanwhile, the content of biological accessible polyphenols is also increased, and the blood sugar level of a diabetic patient can be effectively reduced.

The invention discloses a kudzuvine root powder-tea polyphenol compound and a preparation method thereof and a solid beverage, and relates to the technical field of kudzuvine root powder modification.The kudzuvine root powder-tea polyphenol compound comprises modified kudzuvine root powder particles and tea polyphenol, and the crystal form of the modified kudzuvine root powder particles is of a V-shaped crystal structure; at least part of the tea polyphenol is embedded into a spiral cavity of amylose of the modified radix puerariae powder particles; the kudzuvine root powder-tea polyphenol compound is granular. The amylose spiral cavity of the V-shaped granular kudzuvine root powder can be connected with a water molecule structure, so that the water solubility of the kudzuvine root powder is improved, and the viscous taste of the brewed natural kudzuvine root powder can still be maintained after gelatinization; the tea polyphenol is embedded in the amylose spiral cavity of the V-shaped granular kudzuvine root powder, so that the tea polyphenol can be effectively protected to play a role in inhibiting hydrolysis and digestion of the kudzuvine root powder after entering intestinal tracts, and the increase of postprandial blood sugar is inhibited. Therefore, the novel kudzuvine root powder-tea polyphenol compound provided by the invention has relatively good cold water solubility, reconstituability and digestion resistance.

Provided herein are methods for the enzymatic production of gamma-cyclodextrin from sucrose. In some cases, the methods involve contacting sucrose with one or more enzymes to convert sucrose to amylose, followed by contacting the amylose with one or more enzymes to convert the amylose to gamma-cyclodextrin. In some cases, the methods produce higher yields of gamma-cyclodextrin relative to alpha-cyclodextrin, beta-cyclodextrin, or both.

The invention discloses an extraction and separation process of barley endosperm, and belongs to the field of grain extraction. The method specifically comprises the following steps that barley grains are cleaned and smashed in spring, then classified screening, hulling and sorting are conducted, pretreated endosperm powder is obtained through grinding, endosperm separation and drying are conducted, complete barley grains and broken or defective barley grains can be separated through classified screening, and reduction of loss of raw materials in the subsequent hulling and sorting process is facilitated. A separation accelerant is added in the endosperm separation process, calcium ions can react with hydroxyl contained in starch molecules to form a complex structure, the stability of starch particles is enhanced, the sugar alcohol compound can inhibit gelling of the starch molecules, especially amylose, through the competitive effect, the recovery rate of starch in barley endosperm is increased, and the yield of the barley endosperm is increased. And the centrifugal liquid obtained by separation in the separation process enhances the recovery of the by-product beta-glucan under the action of the selective adsorbent, reduces the influence of impurity molecules, and improves the recovery rate and purity of the beta-glucan.

The invention provides a preparation method of puffed cereals for drinks, the puffed cereals for drinks and drinks, and the preparation method comprises the following steps: adding grease into compound syrup of which preparation raw materials comprise white granulated sugar and maltose, and uniformly stirring to obtain sugar-oil mixed slurry; adding a puffed cereal raw material into the sugar and oil mixed slurry, and uniformly stirring to obtain a mixed raw material; and baking the mixed raw material, and cooling to obtain the puffed grains for the beverage, wherein the puffed cereal raw materials comprise puffed cereals of a first kind of cereals, and the amylose content in the first kind of cereals accounts for more than 35% of the total starch in percentage by weight. The puffed cereal for the beverage prepared by the embodiment of the invention has low hydroscopicity when the beverage is brewed, and can keep crisp taste.

To provide a noodle composition that suppresses deterioration of workability and appearance during noodle making and cooking, as well as deterioration of the viscoelastic texture characteristic of noodles when eaten, and in particular, suppresses changes in texture over time during refrigerated and frozen storage. [Solution] A noodle composition comprising (A) wheat flour with an amylose content of 8 to 18% by mass, and (B) modified starch.

The utility model provides a multi-parameter rice quality rapid detection device, and aims to solve the problem that a rice quality detection device in the prior art can only detect one parameter independently and does not have the capability of detecting multiple parameters at one time. The device mainly comprises a rotary carrying module, an appearance detection module, a moisture detection module, an amylose content detection module, an aging smell detection module and a material receiving turnover table, according to the utility model, accurate transfer and positioning of rice samples among the detection modules are realized through the rotary carrying module, and rapid detection of multiple parameters such as rice appearance, moisture, amylose content, aging smell and the like is realized in combination with the appearance detection module, the moisture detection module, the amylose content detection module and the aging smell detection module; the device has the advantages of compact size, high integration level, high automation degree and the like, and can significantly improve the efficiency and precision of rice quality detection.

The invention belongs to the technical field of analytical chemistry, and particularly relates to a method for detecting N-fluorenylmethoxycarbonyl protected histidine and enantiomers thereof, which comprises the following steps: (1) preparing a system applicability solution; (2) injecting the system applicable solution into a high performance liquid chromatograph, and separating by adopting a chiral chromatographic column which takes amylose-tri (3, 5-dimethyl phenyl carbamate) covalently bonded porous silica gel microspheres as a stationary phase; (3) carrying out gradient elution by adopting a binary mobile phase system containing a mobile phase A and a mobile phase B; (4) obtaining a chromatogram map; and (5) calculating the contents of the N-fluorenylmethoxycarbonyl protected histidine and the enantiomer according to the chromatogram. The invention discloses a method for detecting Fmoc-His (Trt)-OH and an enantiomer thereof by an HPLC (High Performance Liquid Chromatography) method. The method has the advantages of very high sensitivity and separation degree, good repeatability and durability, simplicity in operation and stable and reliable result.

This invention discloses a centrifugal device and method for detecting amylose content, comprising a centrifugal drum with several evenly distributed connecting lugs on its surface; a hanging plate that rotates up and down is mounted on the connecting lugs via a rotating shaft; a reagent tube, which is installed parallel to the end of the hanging plate and rotates up and down synchronously with the hanging plate; and a centrifuge, which drives the centrifugal drum to rotate, and the hanging plate and reagent tube rotate to be perpendicular to the surface of the centrifugal drum due to centrifugal force during the rotation of the centrifugal drum; the tilt angle of the reagent tube dynamically adjusts with the increase of centrifugal speed. At low speed start-up, the reagent tube maintains a reasonable tilt angle to ensure smooth sample start-up and initial stratification; as the speed gradually increases to high speed, the reagent tube naturally tends to be horizontal under the action of centrifugal inertial force. At this time, the centrifugal force acts on the sample along the axial direction of the reagent tube to the maximum extent, making the sample sedimentation path longer and the sedimentation more complete, effectively eliminating sedimentation dead zones.

The invention relates to a detection method of a morphonidazole isomer. The method is a high performance liquid chromatography method, a chromatographic column with amylose-tri (3-chloro-5-methyl phenyl carbamate) covalently bonded on the surface of silica gel as a filler is adopted, a mobile phase A is a 0.05 mol / L monopotassium phosphate solution, the pH value is adjusted to 2.0 + / -0.5 with phosphoric acid, a mobile phase B is acetonitrile, and the mobile phase A and the mobile phase B are subjected to isocratic elution according to the volume ratio of (20-30): (80-70). The detection method provided by the invention is simple and convenient to operate, can effectively separate R and S isomers of morphonidazole, and can accurately determine the content of the isomers.

This invention aims to provide a method for preparing a "shell-core" starch-based intelligent delivery carrier for blueberry polyphenols. The method mainly includes the following steps: (1) Preparation of porous "core": Natural starch is porousized by enzymatic treatment to construct a porous starch "core" that can efficiently adsorb and load blueberry polyphenols; (2) Construction of surface crystallized "shell": The obtained porous "core" is microgelatinized on the surface and used as a crystallization template. Linear amylose that has been debranched by enzyme is added. By precisely controlling key parameters such as crystallization temperature, the ratio of crystal core to linear amylose, and the distribution of amylose chain length, a dense and rigid crystallized "shell" layer is induced on the surface of the porous "core"; (3) Assembly of intelligent delivery system: Blueberry polyphenols are used as functional objects, and the aforementioned "shell-core" structured starch is used as a carrier. By systematically adjusting the pore size distribution, specific surface area of ​​the "core", and the topology and crystallinity of the "shell", the efficient loading, stable encapsulation, and controllable release and targeted delivery of blueberry polyphenols under specific environments are achieved. The process of this invention is highly controllable, the conditions are mild, and the raw materials are green and safe. The delivery carrier prepared has good loading efficiency, storage stability and responsive release performance, and has broad application prospects in functional foods, health products and pharmaceutical preparations.

The invention discloses prepackaged fresh-keeping wet rice noodles with a normal-temperature shelf life of 12 months and a preparation method of the prepackaged fresh-keeping wet rice noodles. 30-90% of early indica rice and 70-10% of corn starch or wheat starch are used as ingredients, so that the total content of amylose in the mixed raw materials is greater than or equal to 21.5%; adding lactic acid bacteria, and performing constant-temperature and constant-humidity solid-state Steaming the extruded rice vermicelli for more than two times; soaking the acidified vermicelli with lactic acid drinking water; softening and acidifying the vermicelli with hot water at 59-70 DEG C; and weighing, bagging, packaging, sterilizing, cooling and warehousing. The rice noodles are safe in microorganism and physicochemical indexes, are not naturally broken, and are quick and convenient to brew / cook; the preparation method enhances the toughness of the vermicelli, and the vermicelli is not easy to break; steaming for multiple times to inhibit retrogradation; through three high-temperature processes of squeezing, steaming and high-temperature sterilization, the prepared rice noodles can resist high temperature; the sterilization is thorough, and the shelf life is longer.

This invention discloses a method for preparing hard carbon anode materials derived from esterified modified starch and their application in sodium-ion batteries, belonging to the field of sodium-ion battery anode technology. The invention first performs a one-step hydrothermal pretreatment on inexpensive cross-linked-esterified composite modified starch, followed by a one-step high-temperature carbonization to obtain spherical hard carbon anode materials. The compact packing density formed by the spherical morphology facilitates the shortening of ion and electron diffusion paths. Because acetyl groups replace some hydroxyl groups within the starch molecule, the amylose content is reduced, enhancing the structural stability of the starch and improving sodium storage performance. Furthermore, the hard carbon anode sheet is pre-sodium-treated using liquid-phase pre-sodiumization technology, improving the battery's initial efficiency and promoting long-cycle stability. This preparation process is simple, requires basic equipment, has good repeatability, requires no extensive post-processing, and is suitable for large-scale production.

This invention discloses a Wx method for regulating the amylose content of rice. e Alleles, applications, detection primers, ancestral alleles of the Wx gene. lv As shown in SEQ ID No. 4, Wx e The alleles are shown in SEQ ID No. 5, Wx e Alleles compared to the ancestral allele type Wx lv It lacks 240 bases as shown in SEQ ID No. 1. Utilizing the Wx of the present invention... e The allele can regulate the amylose content of rice to 6.1%–7.8%, significantly improving the eating quality of rice and having important breeding value.

The present invention relates in particular to capsules for preparing beverages by introducing water into the capsule. The capsule comprises a molded body of powder or a mixture of powders. The molded body is coated with at least one layer containing at least one starch and at least one fatty acid. The at least one starch is an amylose-containing starch having an amylose content (w / w) of preferably 10% to 40%, preferably 14% to 40%, and most preferably 14% to 25%. The present invention also relates to a method for manufacturing such capsules and the use of such capsules.

The invention discloses functional bioactive glass as well as a preparation method and application thereof, and relates to the technical field of regenerative medical materials.The functional bioactive glass provided by the invention takes silicon, calcium, phosphorus and sodium as basic components, a porous structure is constructed by introducing nano zinc oxide (ZnO), and hydrophilicity and dispersity are improved by utilizing short amylose surface grafting. The nano zinc oxide is used as a pore-forming template and is selectively dissolved out in the subsequent steps to form sphere-like pores with the pore diameter of 1-30nm and the porosity of 30-70%, so that physical support is provided for cell adhesion and bone tissue ingrowth. The short amylose is grafted on the surface of the glass through a covalent bond of an amino silane coupling agent, and hydroxyl (-OH) on a molecular chain of the short amylose and water molecules form a hydrogen bond, so that the contact angle of the material is obviously reduced, and particle aggregation is effectively inhibited.

This invention relates to the field of plant genetic engineering, and in particular to a method for increasing the total starch content and / or the proportion of amylose in plant tubers. This invention is the first to clone a 5'-AMP-activated protein kinase gene, named StPTST1, from the potato cultivar "Atlantic." Its full-length cDNA sequence is 840 bp, encoding a protein composed of 279 amino acids. Overexpression of StPTST1 in potatoes and tobacco increases the total starch content in the tubers, and also significantly increases the proportion of amylose. Using this invention, new plant varieties with specific starch qualities can be rapidly cultivated to meet diverse industrial processing and consumer demands.

This method for producing noodles comprises a step for preparing a dough using a powder starting material. 5-25% by mass of the powder raw material is a gelatinized wheat flour obtained by performing a gelatinization treatment on a raw material wheat flour derived from wheat (low-amylose wheat) in which one or more amylose synthesis genes (Wx-A1, Wx-B1, and Wx-D1) have been deleted. As an example of the gelatinization treatment, the method may include a step of mixing 100 parts by mass of the raw material wheat flour and 30-300 parts by mass of water, and heating the mixture under conditions where the temperature of the mixture is 70 DEG C or higher.

The preparation method comprises the following steps: mixing vital gluten and 65% ethanol according to a mass ratio of 1: 30, and stirring and extracting in a water bath kettle at 35 DEG C for 3 hours to dissolve alcohol-soluble protein in the vital gluten in the ethanol; after standing, centrifuging for 5 minutes by using a centrifugal machine under the condition of 3,500 r / min, taking the precipitate, repeating the steps for dissolving, centrifuging the solution for 5 minutes, and taking the precipitate, namely the glutenin aggregate. Dissolving the glutenin aggregate at 30 DEG C for 120 minutes by using 0.1% sodium hydroxide alkali liquor, standing at 50 DEG C for 12 hours, and regulating the pH value to be neutral to generate precipitate; and centrifuging for 5 minutes to obtain a precipitate which is alkali-soluble glutenin. Amylose and alkali-soluble glutenin are mixed according to the mass ratio of 1: 8-1: 10, drinking water accounting for 3%-5% of the starch amount is added, mixing is conducted for 30-50 min at the temperature of 30-35 DEG C, and the disulfide bond content of the alkali-soluble glutenin is reduced by 80% or above.

The invention discloses a recombinant bacillus subtilis strain and application thereof, and belongs to the technical field of biology. The recombinant bacillus subtilis BLCY-017 disclosed by the invention is preserved in the China Center for Type Culture Collection on November 17, 2025, the address is No. 299 on eight road in Wuchang District, Wuhan City, Hubei Province, and the strain preservation number is CCTCC NO: M 20252557. The recombinant bacillus subtilis BLCY-017 can produce starch debranching enzyme, is used for producing resistant starch, can act in starch with the DE value of 2-3%, cuts off alpha-1, 6 glucosidic bonds of starch branches, can cut off long-chain branch structures, reduces the generation of small molecules, obtains long-chain high-amylose starch, improves the dietary fiber content through an annealing process, and improves the dietary fiber content. The digestion rate is reduced, and the viscosity and the water binding capacity of the starch can be better reserved.