17 results about "Amylose" patented technology

The invention provides a preparation method of puffed cereals for drinks, the puffed cereals for drinks and drinks, and the preparation method comprises the following steps: adding grease into compound syrup of which preparation raw materials comprise white granulated sugar and maltose, and uniformly stirring to obtain sugar-oil mixed slurry; adding a puffed cereal raw material into the sugar and oil mixed slurry, and uniformly stirring to obtain a mixed raw material; and baking the mixed raw material, and cooling to obtain the puffed grains for the beverage, wherein the puffed cereal raw materials comprise puffed cereals of a first kind of cereals, and the amylose content in the first kind of cereals accounts for more than 35% of the total starch in percentage by weight. The puffed cereal for the beverage prepared by the embodiment of the invention has low hydroscopicity when the beverage is brewed, and can keep crisp taste.

This invention discloses a centrifugal device and method for detecting amylose content, comprising a centrifugal drum with several evenly distributed connecting lugs on its surface; a hanging plate that rotates up and down is mounted on the connecting lugs via a rotating shaft; a reagent tube, which is installed parallel to the end of the hanging plate and rotates up and down synchronously with the hanging plate; and a centrifuge, which drives the centrifugal drum to rotate, and the hanging plate and reagent tube rotate to be perpendicular to the surface of the centrifugal drum due to centrifugal force during the rotation of the centrifugal drum; the tilt angle of the reagent tube dynamically adjusts with the increase of centrifugal speed. At low speed start-up, the reagent tube maintains a reasonable tilt angle to ensure smooth sample start-up and initial stratification; as the speed gradually increases to high speed, the reagent tube naturally tends to be horizontal under the action of centrifugal inertial force. At this time, the centrifugal force acts on the sample along the axial direction of the reagent tube to the maximum extent, making the sample sedimentation path longer and the sedimentation more complete, effectively eliminating sedimentation dead zones.

This invention aims to provide a method for preparing a "shell-core" starch-based intelligent delivery carrier for blueberry polyphenols. The method mainly includes the following steps: (1) Preparation of porous "core": Natural starch is porousized by enzymatic treatment to construct a porous starch "core" that can efficiently adsorb and load blueberry polyphenols; (2) Construction of surface crystallized "shell": The obtained porous "core" is microgelatinized on the surface and used as a crystallization template. Linear amylose that has been debranched by enzyme is added. By precisely controlling key parameters such as crystallization temperature, the ratio of crystal core to linear amylose, and the distribution of amylose chain length, a dense and rigid crystallized "shell" layer is induced on the surface of the porous "core"; (3) Assembly of intelligent delivery system: Blueberry polyphenols are used as functional objects, and the aforementioned "shell-core" structured starch is used as a carrier. By systematically adjusting the pore size distribution, specific surface area of ​​the "core", and the topology and crystallinity of the "shell", the efficient loading, stable encapsulation, and controllable release and targeted delivery of blueberry polyphenols under specific environments are achieved. The process of this invention is highly controllable, the conditions are mild, and the raw materials are green and safe. The delivery carrier prepared has good loading efficiency, storage stability and responsive release performance, and has broad application prospects in functional foods, health products and pharmaceutical preparations.

This invention relates to the field of plant genetic engineering, and in particular to a method for increasing the total starch content and / or the proportion of amylose in plant tubers. This invention is the first to clone a 5'-AMP-activated protein kinase gene, named StPTST1, from the potato cultivar "Atlantic." Its full-length cDNA sequence is 840 bp, encoding a protein composed of 279 amino acids. Overexpression of StPTST1 in potatoes and tobacco increases the total starch content in the tubers, and also significantly increases the proportion of amylose. Using this invention, new plant varieties with specific starch qualities can be rapidly cultivated to meet diverse industrial processing and consumer demands.

The application discloses a method for predicting rice glycemic index in vitro, and belongs to the technical field of food detection. The application collects multi-dimensional data of rice in the whole life cycle of "raw materials-processing-sensory-digestion", screens four key influence factors of amylose content (AM), palatability value (PS), disintegration value (BD) and area under the curve of 0-120 min digestion (AUC120) through AI-assisted machine learning, and constructs a nonlinear regression prediction model. The model realizes accurate prediction of GI with multi-dimensional feature fusion, and is highly consistent with the test results of ISO26642:2010 standard human body (R<2>=0.965). The application has the advantages of simple operation, low cost, short cycle and the like, and can be used for rapid screening of low GI rice varieties, assisted breeding and quality control of functional rice products.

The application provides a rice product raw material quality prediction device and application, and relates to the technical field of food quality detection and control. The method first divides rice product raw materials into several groups according to weight, extracts equal-weight raw materials from each group as detection samples, and uses a Japanese Sakuradake particle evaluator to detect cracks, yellowing and insect-damaged particles in the samples and weigh them. At the same time, the samples are ground into three equal parts, and the Kjeldahl method, oven drying method and iodine colorimetric method are used to determine the contents of protein, moisture and amylose in the samples, respectively. The infrared spectroscopy imaging technology is used to record the absorbance of the samples at a specific waveband, and a prediction model of the contents of protein, moisture and amylose is established based on the partial least squares method. According to different evaluation standards, the limitations of traditional single-index detection are avoided, and the stable high quality of products in terms of taste, nutritional value, appearance and shelf life is ensured.

This invention discloses a Bacillus subtilis ( Bacillus subtle The EB-8 strain, deposited on January 14, 2026, at the China General Microbiological Culture Collection Center (CGMCC) with accession number CGMCC No. 37320, is disclosed. The application of Bacillus subtilis strain EB-8 in the fermentation modification of tartary buckwheat flour is also disclosed. This invention's Bacillus subtilis strain EB-8 can significantly increase the amylose content of tartary buckwheat, helping to inhibit starch retrogradation and improve its freeze-thaw stability; simultaneously, it increases the total flavonoid and total phenolic content of tartary buckwheat, enhancing its antioxidant activity. Furthermore, this strain can selectively retain and enrich essential amino acids such as lysine and methionine, reduce bitter components, improve taste, and facilitate the formation of a cereal matrix suitable for cold processing, with a mild taste and enhanced oxidative defense. Overall, it achieves synergistic optimization of the functional activity and processing characteristics of tartary buckwheat flour, which is beneficial for the high-value utilization of tartary buckwheat flour resources.

This utility model relates to an ultrasonic amylose extraction device, comprising an extraction cylinder, an agitation component inside the extraction cylinder, the upper end of the agitation component extending to the top of the extraction cylinder and connected to a transmission mechanism, a feeding hopper connected to the top of the extraction cylinder, and the bottom of the agitation component connected to the bottom of the inner cavity of the extraction cylinder. A drive mechanism and an ultrasonic power supply are also provided at the top of the extraction cylinder. The agitation component includes a transmission rod passing through the inside of the extraction cylinder, and a plurality of uniformly distributed ultrasonic transducers are fixedly connected to the outer surface of the transmission rod. This utility model, with the assistance of the power supply and a conductor, enables the ultrasonic transducers to output ultrasonic vibrations with the assistance of a flow guiding component. This allows the mixture inside the extraction cylinder to be fully agitated by the flow guiding component, increasing the efficiency of cell movement in the mixture and significantly improving the efficiency of ultrasonic cavitation effect on cells, thereby effectively improving the efficiency of extracting intracellular substances.

The application belongs to the field of rice genetic breeding, and discloses a rice SBEIIb mutant protein and application thereof in changing starch crystal structure and increasing resistant starch content. The mutant protein simultaneously contains double-amino-acid substitution (M610I / D611N) of substitution of M at the 610th position to I and substitution of D at the 611th position to N. The mutant protein can change rice starch from A-type to C B -type, significantly increase amylose content from 13.8% of the wild type to 20.3%, and significantly increase starch gelatinization temperature. Meanwhile, the mutant protein (M610I / D611N) can increase the resistant starch content in rice by about 5 times; compared with the ae mutant with SBEIIb deletion, the grain width, grain thickness and grain weight are significantly increased, and the chalky kernel rate, chalky area and chalkiness are significantly reduced. The SBEIIb-M610I / D611N in the application has important application value in cultivating functional rice varieties rich in resistant starch.

This invention discloses a soil conditioner for saline-alkali land and its preparation method, belonging to the field of saline-alkali land improvement technology. Its core carriers are chitosan, β-cyclodextrin, and debranched short-chain starch, all of which are natural polysaccharide biomass materials. These can be gradually degraded by bacteria in the soil and possess good and long-lasting salt control effects, alleviating soil compaction and improving the crop growth environment. Debranched starch-modified chitosan composite microspheres are used as the main active ingredient. Through a porous structure, zero-valent iron is fixed inside, achieving stable dispersion and loading of zero-valent iron, allowing heavy metal ions to fully contact the adsorption sites. Furthermore, the hydroxyl groups of debranched starch and the amino groups of chitosan themselves have coordination adsorption capabilities, which, combined with the cavity inclusion effect of cyclodextrin, form multiple coordination adsorption effects, further enhancing the heavy metal adsorption capacity. Moreover, the branches of debranched starch form steric hindrance on the surface of zero-valent iron, which, together with the electrostatic repulsion of chitosan and the cavity isolation of cyclodextrin, prevents aggregation.

The application provides a method for determining chiral isomers of pilocarpine hydrochloride by high performance liquid chromatography and application thereof, relates to the technical field of drug analysis, and comprises detecting and analyzing a pilocarpine hydrochloride sample solution by high performance liquid chromatography; wherein a chromatographic column of the high performance liquid chromatography is filled with amylose-tris (3, 5-dimethylphenylcarbamate) silica gel; and a mobile phase of the high performance liquid chromatography comprises n-hexane, methanol and an ethanol solution containing an organic base. The application uses amylose-tris (3, 5-dimethylphenylcarbamate) silica gel as the filling agent of the chromatographic column, and uses a n-hexane-ethanol solution (0.1% diethylamine)-methanol solution with a certain proportion as the mobile phase, so that the separation and analysis of pilocarpine hydrochloride and chiral isomers can be realized, and thus the quality of pilocarpine hydrochloride and its preparation can be accurately controlled.

The present application relates to the technical field of biological catalysis, and discloses a method for converting straw into amylose, which comprises the following steps: pretreating straw by using a pretreatment system composed of choline chloride / ethylene glycol and NaHCO3 to remove lignin, then washing and drying the pretreated straw as a substrate, then constructing a multi-enzyme synergistic catalytic system, which comprises an engineered bacterium whole-cell catalyst obtained by using ice nucleation protein to fix and display CBP1942 and PGP on the surface of E. coli, and a cellulase preparation with low beta-glucosidase activity, then adding one or more of molybdate, vanadate and pyrophosphate into the catalytic system, and finally converting the substrate into amylose under suitable reaction conditions in the catalytic system. The method effectively improves the lignin removal rate, enhances the multi-enzyme synergistic effect, and inhibits the degradation of phosphorylated intermediates, so that the yield of amylose is increased by about 5 times.

This invention discloses a breeding method for long-spike, low-amylose-content rice. The method includes: hybridization using long-spike, large-grain Yiyang Dahe rice as the female parent and low-amylose-content japonica rice as the male parent; backcrossing the F1 generation seeds with the female parent once; planting for three consecutive generations; and performing molecular marker detection on the BC1F4 individual plant lines. Wx This invention involves inserting repetitive sequences into genes, designing dominant marker W1 and / or co-dominant marker W2 to detect the inserted repetitive sequences, and determining whether the corresponding BC1F4 line has a glutinous phenotype. The progeny lines obtained by planting glutinous BC1F4 single plants through multiple generations exhibit non-segregating phenotypes. This invention solves the problem of existing technologies failing to improve the amylose content of Dahu rice and thus being unsuitable for producing high-quality Yiyang rice cakes. By combining traditional hybridization-backcrossing methods with RVA viscosity detection, molecular marker-assisted selection, and phenotypic identification breeding techniques, a new Bai Ruyu Dahu rice variety with long spikes, large grains, disease resistance, and low amylose content can be rapidly and accurately bred.

This invention discloses the application of the tobacco NtGBSS2 gene in regulating starch synthesis and / or drought resistance, and in improving tobacco germplasm resources. It belongs to the field of plant genetic engineering technology. The nucleotide sequence of the coding region of the tobacco NtGBSS2 gene is shown in SEQ ID NO.1. This invention overexpressed the NtGBSS2 gene in tobacco. Detection revealed that, compared to normal control tobacco plants, the content of resistant starch and amylose in the transgenic positive T1 generation was significantly increased. Furthermore, observation showed that wild-type control tobacco plants grew slowly and had yellowing leaves under drought stress, while the growth status of transgenic positive T2 generation tobacco plants was significantly improved compared to wild-type control tobacco plants, indicating that overexpression of the NtGBSS2 gene in tobacco enhances the tobacco's tolerance to drought stress.

This invention discloses a wheat TaGRAS11a and TaGRAS11b CRISPR / Cas9 gene editing vectors and their applications: The vectors use pBUE414 as a backbone and contain a Cas9 expression cassette and an sgRNA expression cassette. The Cas9 expression cassette is the expression cassette that regulates Cas9 expression via the Ubi promoter. The sgRNA expression cassette includes the following elements from upstream to downstream: TaU3 promoter, targeting... TaGRAS11a sgRNA1, terminator OsU3t, TaU3 promoter, target TaGRAS11b The sgRNA2 terminator, TaU3t. This invention targets the hexaploid wheat genome. TaGRAS11a and TaGRAS11b A dual-target design was implemented using conserved sequence regions with six homologous copies, enabling simultaneous targeted knockout of all six alleles with a single transformation. The resulting co-knockout mutant grains showed significant reductions in amylose, amylopectin, and total starch content, clarifying the crucial positive regulatory role of this gene family in the wheat grain starch synthesis pathway.

This invention relates to the field of food technology, providing a freeze-thaw stabilizer, its preparation method, and its application in salted frozen desserts. The freeze-thaw stabilizer comprises white soybean dextrin with a viscosity of 200-350 mPa·s and a linear starch content of 33%-40%. Through performance studies of white soybean dextrin, this invention has found that white soybean dextrin within the above performance range possesses excellent freeze resistance and processing fluidity. Furthermore, when this white soybean dextrin is used as a stabilizer in frozen desserts, its strong intermolecular forces allow its molecules to intertwine and form a high-hardness gel after freezing, resulting in better freeze resistance stability. This white soybean dextrin is particularly suitable for the preparation of salted frozen desserts, such as ice cream products.