138 results about "Uronic acid" patented technology

The invention relates to the field of medicines, foods or health-care products, in particular to big-fruit hawthorn heteropolysaccharide as well as a preparation method and application thereof. The large-fruit hawthorn heteropolysaccharide is prepared from galacturonic acid, glucuronic acid, mannose, xylose, arabinose, glucose, galactose, fucose and rhamnose. The weight-average molecular weight of the big fruit hawthorn heteropolysaccharide is 81.6 kDa to 157.3 kDa. The product has unique structures of complex monosaccharide composition, low molecular weight, appropriate uronic acid content, low esterification degree and the like; compared with CP which is single in structure and high in esterification, the CP is better in oxidation resistance and bile acid binding capacity; compared with hawthorn polysaccharide with low uronic acid content and high molecular weight, the hawthorn polysaccharide has the advantages that the enzyme inhibition activity is obviously enhanced, and the hawthorn polysaccharide has a wide prospect in development of functional food, health care products or medicines for regulating blood sugar / blood fat, resisting oxidation and preventing metabolic syndromes.

The invention discloses hippophae rhamnoides polysaccharide with the activity of reducing blood sugar and blood fat as well as a preparation method and application thereof. Adding water into the degreased hippophae rhamnoides, adjusting the pH value to be acidic, then heating, stirring and extracting under an ultrasonic condition, and collecting filtrate; and performing post-treatment to obtain the sea-buckthorn polysaccharide. The content of neutral sugar in the sea buckthorn polysaccharide is 25.31%-30.68%, the content of uronic acid in the sea buckthorn polysaccharide is 54.66%-59.79%, and the content of protein in the sea buckthorn polysaccharide is 1.91%-2.14%. According to the method, the sea-buckthorn polysaccharide is extracted by adopting acid assistance-ultrasonic extraction, and the prepared sea-buckthorn polysaccharide has remarkable blood sugar and blood fat reducing activity. When the concentration of the sea-buckthorn polysaccharide is as low as 0.1 mg / mL, the glucose uptake of HepG2-IR cells can still be obviously improved, and the content of total cholesterol and total triglyceride of the HepG2-IR cells can be obviously reduced. The hippophae rhamnoides polysaccharide can be applied to preparation of food or medicine for reducing blood sugar and / or blood fat.

The invention provides an application of a detection reagent of five tryptophan metabolites in preparation of an early diagnosis kit for ER (estrogen receptor) positive breast cancer. The tryptophan metabolites are kynurenine Kyn, indole-3-carboxylic acid glucuronide I3GA, indole-3-acetic acid I3A, indole-3-acetyl-L-alanine IAA-L-Ala and L-tryptophan L-Trp. According to the invention, early accurate diagnosis of ER positive breast cancer is realized, and the technical problems of insufficient efficiency of traditional diagnosis means and lack of'combined application 'in existing metabolite research are solved.

The application discloses a kind of acanthopanax acid polysaccharides with intestinal protection function and its preparation method and application, belong to the field of functional food and biotechnology.The acanthopanax acid polysaccharide is by water extraction alcohol precipitation, after impurity removal, through DEAE-52 anion exchange column elution and collection 0.2 mol / L NaCl elution peak, and the uniform component obtained by Sephadex G-100 gel column chromatography purification.Its uronic acid content is 30%-40%, weight average relative molecular weight is 50-100kDa, mainly by glucose, galactose, galacturonic acid and arabinose, infrared spectrum has carboxyl characteristic absorption peak at 1736 cm-1 and 1615 cm-1.Experiments prove that the acanthopanax acid polysaccharide has antioxidant activity and hypoglycemic activity.The acid polysaccharide has protective effect on LPS-induced Caco-2 cell damage, can effectively reduce the secretion of pro-inflammatory factors TNF-α, IL-1β, IL-6, and improve the level of anti-inflammatory factor IL-10.The acanthopanax acid polysaccharide can be prepared into oral liquid, tablet candy, solid beverage and other functional food forms.

Provided is a composition for inhibiting the proliferation of bacteria belonging to the genus Collinsella, the composition containing at least one selected from sugar acid-containing oligosaccharides and salts thereof. The sugar acid is preferably selected from aldonic acid, uronic acid, and aldaric acid.

This invention discloses the application of Uncaria rhynchophylla neutral polysaccharide URP-W in the preparation of anti-glioma drugs, belonging to the field of anti-tumor technology of medicinal plant polysaccharides. The invention obtains a crude polysaccharide extract from Uncaria rhynchophylla through hot water extraction and ethanol precipitation, followed by purification using a DEAE cellulose column and G200 dextran gel to obtain Uncaria rhynchophylla neutral polysaccharide URP-W. The monosaccharide composition of URP-W includes arabinose, rhamnose, galactose, glucose, xylose, mannose, galacturonic acid, and mannuronic acid in a molar ratio of 17.99:6.32:21.71:27.17:2.56:7.57:16.20:0.49. Experiments have shown that URP-W can inhibit the proliferation of gliomas and induce apoptosis, exhibiting significant anti-tumor activity against glioma cells, providing a new drug raw material and treatment method for the clinical treatment of gliomas.

The invention relates to the cosmetic and non-therapeutic use of a composition consisting essentially of a compound of formula I below: (I) or its enantiomers, isomers or diastereoisomers, or a mixture thereof, or a salt or a solvate thereof, wherein R1 and R2, independently of each other, are a C3-C6 sugar, a uronic acid, or a glucoside, for bleaching or whitening the skin and skin appendages of a healthy individual.

Provided is a glucuronidase activator containing, as an active ingredient, Levilactobacillus brevis (Levilactobacillusbrevis) strain I-3141 (accession number: CECT 7480).

The invention belongs to the technical field of plant polysaccharide, and particularly discloses caulis spatholobi polysaccharide, a preparation method thereof and application of the caulis spatholobi polysaccharide in preparation of medicines for treating inflammation. Monosaccharide in the caulis spatholobi polysaccharide disclosed by the invention comprises mannose, glucuronic acid, galacturonic acid, glucose and arabinose in a molar ratio of (0.5-0.6): (0.3-0.5): (1-2): (1-2): (0.5-1.5). The caulis spatholobi polysaccharide provided by the invention is a novel uniform-structure polysaccharide which is separated from caulis spatholobi rattan for the first time, has a slightly acidic pH value, definite monosaccharide composition, definite molecular weight and clear glycosidic bond linking mode, has an anti-inflammatory effect, and is expected to be developed into a new anti-inflammatory drug. The preparation process is mild in condition, simple to operate and environment-friendly.

This invention relates to a method for identifying and detecting urinary biomarkers of whole-grain wheat intake. These biomarkers include glycine conjugates of 3,5-dihydroxybenzoic acid, sulfate conjugates of o-aminophenol, sulfate conjugates of 2-acetaminophen, sulfate conjugates of 3,5-dihydroxyphenylvaleric acid, glucuronic acid conjugates of o-aminophenol, or glucuronic acid conjugates of 2-acetaminophen. The invention also relates to a high-throughput screening method for urinary biomarkers of whole-grain wheat intake. A whole-grain wheat dietary intervention experiment was designed, and through comparative analysis of human urinary metabolic profiles, non-targeted metabolomics technology was used to screen for urinary metabolic biomarkers of whole-grain wheat, providing a method for accurately assessing whole-grain wheat intake.

This invention discloses a preparation process for a homogeneous immunomodulatory polysaccharide from *Gynostemma pentaphyllum* and its application in the preparation of anti-melanoma drugs and immunomodulators, relating to the field of pharmaceutical manufacturing technology. The preparation process includes four steps: compound enzyme-ultrasound synergistic extraction, fractional alcohol precipitation to remove proteins, DEAE-52 ion exchange column chromatography, and Sephacryl S-400 gel permeation chromatography purification. The resulting homogeneous polysaccharide (GOP-3) has a weight-average molecular weight of 12±1 kDa and is composed of mannose, glucose, and galactose in a specific ratio. The GOP-3 of this invention has a weight-average molecular weight (Mw) of 12.8 kDa, a dispersion (Mw / Mn) < 1.2, a uronic acid content of 23.5%, a well-defined structure, and is suitable for drug application. Furthermore, GOP-3 can significantly promote the secretion of IL-12 by dendritic cells and induce the differentiation of naive T cells into Th1 cells. Simultaneously, compared with a full-dose PD-1 monoclonal antibody, the combination of GOP-3 and a half-dose monoclonal antibody not only reduces the risk of immune-mediated pneumonia caused by antibody drugs but also increases the tumor inhibition rate by more than 40%.

The invention belongs to the technical field of traditional Chinese medicine, and relates to polygonum cuspidatum homogeneous polysaccharide and a preparation method and application thereof.The polygonum cuspidatum homogeneous polysaccharide is mainly composed of galacturonic acid, rhamnose, galactose, glucuronic acid, glucose, mannose, xylose and glucuronide amine according to the molar ratio of 41.967: 24.624: 17.951: 5.427: 4.59: 3.501: 1.236: 0.358, and the weight-average molecular weight of the polygonum cuspidatum homogeneous polysaccharide is 26.57 kDa. The polygonum cuspidatum homogeneous polysaccharide is fixed in component, high in yield, good in hypoglycemic effect and good in safety, and a basis is provided for development of innovative hypoglycemic drugs.

The invention relates to the technical field of cosmetics, in particular to acer truncatum moisturizing pre-makeup cream and a preparation method thereof. The acer truncatum moisturizing makeup pre-cream is prepared from the following components in parts by weight: acer truncatum seed oil, cosmetic-grade purified water, cetyl PEG / PPG-10 / 1 polydimethylsiloxane, polydimethylsiloxane, isononyl isononanoate, synthetic wax, microcrystalline wax, glycerol, 1, 2, 4-trimethyl-1, 3-pentanediol monoisobutyrate, propylene glycol monobutyl ether, propylene glycol monobutyl ether, propylene glycol monobutyl ether, propylene glycol monobutyl ether, propylene glycol monobutyl ether, propylene glycol the mannuronic acid tetrasaccharide micro-capsule is coated with 1, 3-propylene glycol / caprylyl glycol / ethylhexylglycerin, butanediol, sodium chloride, a sodium hyaluronate cross-linked polymer and temperature-sensitive polyglycerol-10 stearate. The acer truncatum seed oil containing 6% of nervonic acid serves as core lipid, temperature-sensitive type microencapsulated mannuronic acid tetrasaccharide and response type composite microcapsules are compounded, a'instant moisturizing-delayed moisturizing-barrier repairing 'triple synergistic system is constructed, the defects of a traditional technology are overcome, and the requirements of consumers for long-acting, efficient and mild pre-makeup care are met.

The invention discloses a preparation method and application of a quercetin derivative grafted with 5-(4-hydroxybenzyl)-2, 4-thiazolidinedione with a blood sugar reducing function. According to the quercetin-3-O-glucuronide-linked 5-(4-hydroxybenzyl)-2, 4-thiazolidinedione compound disclosed by the invention, the quercetin-3-O-glucuronide-linked 5-(4-hydroxybenzyl)-2, 4-thiazolidinedione compound is prepared by linking quercetin-3-O-glucuronide with 5-(4-hydroxybenzyl)-2, 4-thiazolidinedione, the structural formula of the quercetin-3-O-glucuronide-linked 5-(4-hydroxybenzyl)-2, 4-thiazolidinedione compound is shown as a formula (I), and the quercetin-3-O-glucuronide-linked 5-(4-hydroxybenzyl)-2, 4-thiazolidinedione compound prepared by the invention has the function of reducing blood sugar and can be used for preparing medicines for treating diabetes mellitus.

The invention discloses a keloid treatment gel material capable of reducing lactic acid generation, and belongs to the technical field of keloid treatment. The material is prepared by the following steps: firstly, carrying out esterification on 9-oxabicyclo [3.3. 1] nonane-2, 6-diol and S-allyl-L-cysteine to obtain an intermediate A, and then carrying out Schiff base reaction on the intermediate A and 5-methoxy heliotropin to obtain an intermediate B containing double bonds; then, copolymerizing with methylacryloylated chitosan and polyethylene glycol diacrylate under photo-initiation to form a cross-linked network structure; finally, active ingredients such as quercetin-3-O-glucuronide and luteolin-3 '-glucuronide are included, and auxiliary materials such as a humectant and a preservative are added, so that the composition is prepared. The gel has a slow release function, can effectively inhibit glycolysis, reduce lactic acid generation, scavenge free radicals and regulate inflammation and fibrosis microenvironment, has good biocompatibility and oxidation response drug release characteristics, and is suitable for prevention and treatment of keloids.

The compounds of the invention can be used to stabilize biomolecules, particularly in the presence of pH and thermal stress. Formula (I), wherein X is a hexosyl uronic acid or uronic acid amide group. (I).

This invention discloses a recombinant glucuronide C5 epimerase mutant, expression vector, recombinant expression engineered bacteria, and their culture and induction methods, belonging to the field of biotechnology. The recombinant enzyme sequence is formed by fusing a truncated (N29–N617) mutation (V173R–V197K–A239V–N269E) with an N-terminal maltose-binding protein (MBP) tag to a human glucuronide C5 epimerase (C5-epi). The recombinant glucuronide C5 epimerase mutant exhibits higher activity, 2.1 times that of the recombinant wild-type, and greater stability, retaining 72.5% of its activity after 5 days at room temperature. Furthermore, the soluble expression level of this recombinant mutant glucuronide C5 epimerase is increased by 4-fold.

The invention belongs to the technical field of traditional Chinese medicine extraction, and relates to a nine-steamed nine-processed rhizoma polygonati homogeneous polysaccharide and a preparation method and application thereof, in particular to a nine-steamed nine-processed rhizoma polygonati homogeneous polysaccharide and a preparation method and application thereof. The purpose of the invention is to determine one component in the nine-steamed nine-processed polygonatum sibiricum product. NPCP-A1 is extracted by a water extraction and alcohol precipitation method, deproteinized by a Sevag method, and subjected to chromatographic separation and purification by a DEAE-52 anion exchange column and a Sephacryl S-300 gel column, and the NPCP-A1 is prepared from arabinose, rhamnose, galactose, glucose, xylose, mannose, galacturonic acid and glucuronic acid. The proliferation of RAW264.7 cells and the secretion of NO, TNF-alpha and IL-6 can be promoted. The composition is used for preparing health-care products or medicines for improving immunity. According to the acidic polysaccharide prepared by the method disclosed by the invention, the polysaccharide plays an immune regulation role by promoting macrophage proliferation and increasing secretion of cell factors such as NO, TNF-alpha, IL-6 and the like.

The invention discloses a nutritional composition for promoting brain development and application thereof, and relates to the technical field of biological medicines. The compound composition is selected from a composition A (melatonin + uridylic acid + inositol), a composition B (melatonin + uridylic acid) and a composition C (uridylic acid + inositol). Experiments prove that the combination B can significantly improve elevated anxiety behaviors and learning and memory ability caused by rhythm disorder, the combination C can significantly improve open field anxiety behaviors caused by rhythm disorder, and the combination A can significantly relieve anxiety behaviors (including elevated and open field) and spatial learning and memory ability disorder caused by rhythm disorder at the same time. The active components in the compound composition disclosed by the invention are remarkable in synergistic effect, high in safety and good in biocompatibility, and can be used for preparing medicines, food additives or feed additives for improving rhythm disorder related brain dysplasia.

The present disclosure relates to methods and compositions for quantifying the relative distribution of glucuronidation, iduronidation, and / or galacturonidation of polypeptides, including stereoselective liquid chromatography mass spectrometry (LC-MS) methods that can achieve simultaneous separation and relative quantification of glucuronidation, iduronidation, and / or galacturonidation of polypeptides.

This invention discloses an antitumor natural drug composition based on Norway maple and its preparation method, relating to the field of biomedical technology. The compound is selected from at least one of the following structures: 3-O-[β-D-glucopyranosyl-(1→4)-[β-D-galactopyranosyl-(1→2)]]-β-D-glucopyranoside-21-O-acetyl-22-O-angeloyl yrutile alcohol, with the molecular formula C2. 55 H 86 O 23 This invention isolates a class of triterpenoid saponins with a rutinol skeleton from the bark of Norwegian maple, enriching the chemical composition library of maple species. The compounds contain unique oligosaccharide chains and diacyl substitution patterns at C-21 and C-22. One compound has a rare branched acyl group (3-hydroxy-4-methylhexanoyl) at C-21, while the other compound contains an arabinofuranose unit in its C-3 trisaccharide chain. These structural features are rare in natural products and demonstrate excellent structural novelty.

The present application relates to a method for regulating the growth of calcium carbonate crystals into calcite by glucose uronic acid, so as to improve the mechanical strength of the solidified body. The specific steps of the method are as follows: (1) screening and identification of acetate mineralization bacteria with calcium acetate as substrate; (2) culture of carbonate mineralization strain; (3) growth and regulation of calcium carbonate crystals induced by microorganisms; (4) characterization of calcium carbonate crystals. The microbial solidification of the uranium tailings beach is a green solidification method with great application prospect. The carbonate mineralization strain provided by the present application has no secondary pollution in the process of microbial induction of calcium carbonate precipitation, has strong environmental friendliness, and can regulate the growth of calcium carbonate crystals into calcite by glucose uronic acid, so as to improve the mechanical strength of the calcium carbonate crystals, and has potential application prospect.

The invention discloses a stringy stonecrop herb polysaccharide extraction process which comprises the following steps: drying and crushing stringy stonecrop herb, adding water according to a material-liquid ratio of (1: 20)-(1: 60), extracting, concentrating, and carrying out alcohol precipitation to obtain crude polysaccharide; deproteinizing, dialyzing and freeze-drying; carrying out gradient elution and purification to obtain sedum sarmentosum polysaccharide SSBP; an extraction process is optimized through a response surface method, optimal conditions are selected, and the yield of crude polysaccharide reaches 19.06%; according to structural characterization, SSBP is acidic polysaccharide, the molecular weight is 7.484 kDa, 36.12% of uronic acid is contained, monosaccharide is composed of rhamnose, arabinose, galactose, galacturonic acid and glucuronic acid, and the molar ratio of rhamnose to arabinose to galactose to galacturonic acid to glucuronic acid is 12.85: 19.13: 18.98: 35.73: 3.35; an in-vitro activity experiment shows that the SSBP has good antioxidant activity and remarkable anti-inflammatory activity, the release of RAW264.7 macrophage NO induced by LPS and the secretion of IL-6, IL-1beta and TNF-alpha can be inhibited in a dose-dependent manner, and the inhibition rates under the concentration of 1.2 mg / mL respectively reach 74.41%, 92.53%, 95.27% and 95.73%; and a new scheme is provided for deep development of sedum sarmentosum polysaccharide and preparation of anti-inflammatory and anti-oxidation products.

The invention belongs to the field of daily chemicals, and discloses an anti-wrinkle composition based on lipidosome delivery, which comprises the following components by weight: 0.1-1 part of a Chrysanthemum indicum extract; 0.1 to 1 part by weight of dipalmitoyl hydroxyproline; and 0.1 to 1 part by weight of methylsilanol mannuronate. According to the composition, dipalmitoyl hydroxyproline, a stellera chamaejasme extract and methylsilanol mannuronate serve as active ingredients, liposome serves as a carrier, and the anti-wrinkle effect can be achieved to the maximum extent. Meanwhile, the invention further discloses the lipidosome, application of the lipidosome and cosmetics.

The invention provides an antimycotic polyene-alginate oligomer conjugate comprising an antimycotic polyene connected covalently to at least one alginate oligomer, wherein one or other terminal uronic acid residues of each alginate oligomer in the conjugate is connected to the antimycotic polyene via a direct covalent bond or a covalent molecular linker, or a pharmaceutically acceptable salt, solvate, hydrate, diastereoisomer, tautomer, enantiomer, or active metabolite thereof. Also provided are methods for the preparation of said conjugate, pharmaceutical compositions comprising said conjugate and the use thereof in a method for the treatment or prevention of a fungal infection in a subject with, suspected to have, or at risk of, a fungal infection.

The application discloses a kind of feed additive and its application, it is related to feed additive technical field.The prepared feed additive of the application is by mixing sodium glucuronate, glucomannan, dimethyl cyclodextrin, diatomite and sodium lauryl sulfate, then mixed solution is reacted with calcium chloride crosslinking solution, finally form microspheres type feed additive.Compared with prior art, the prepared feed additive of the application is beneficial to the adsorption of deoxynivalenol and zearalenone, amino acid liquid, lemon yellow and titanium dioxide are mixed, then composite bacteria liquid is added to ferment, by feed additive processing, low-temperature drying can retain the nutrient components and biological activity in raw materials, and the biological fermentation protein raw material with good sensory color is obtained.

The present invention relates to a compound selected from nitazoxanide, tizoxanide, tizoxanide glucuronide and pharmaceutically acceptable salts thereof, for use in a method for the treatment of hepatic impairment.

This invention discloses an instant gelling powder of sodium alginate / calcium β-hydroxy-β-methylbutyrate with dual functions of fat reduction and muscle gain, and its application, belonging to the field of functional food or pharmaceutical preparation technology. The powder of this invention is prepared by dry mixing sodium alginate and CaHMB at a weight ratio of 5~10:1~5, preferably 7:3, wherein the guluronic acid content of sodium alginate is ≥50%. In use, 1g of powder is added to 20~40mL of water at 60~100°C, and stirred to form a hydrogel. After oral administration, the powder rapidly forms a gel in the stomach, producing a feeling of fullness, and simultaneously achieves fat reduction, muscle gain, and metabolic regulation functions by regulating myostatin expression. This invention overcomes the limitation of existing weight management products with only one function, innovatively integrating the three major effects of instant satiety, fat reduction, and muscle gain. The preparation process is simple and environmentally friendly, requiring no added chemical excipients, and has significant technological advancement and market application value.

The present invention relates to a method for detecting glycosidic bonds of plant polysaccharides. The method includes taking 100 μL of NaOH-DMSO suspension (1 mg / mL) of plant polysaccharides containing 20 or more kinds of substances such as galactan, araban, polygalacturonic acid, rhamnogalacturonan-I, rhamnogalacturonan-II, xyluronic acid, arabogalacturonic acid I and arabogalacturonic acid II, shaking at room temperature for 30 minutes, adding 40 μL of iodomethane and shaking for 1 hour. Such process is repeated three times to obtain methylated plant polysaccharides. The method further includes using an automatic detection equipment for methylation combined with PMP derivatization to analyze glycosidic bonds in polysaccharides. Not only the glycosidic bond information of neutral sugars can be analyzed in the subsequent LC-MS mass spectrometry analysis, but also the glycosidic bond information of acidic sugars can be resulted, and the analysis results are faster, more detailed and more reliable.

The invention discloses a wolfberry-astragalus compound polysaccharide with an anti-myelosuppression effect as well as a preparation method and application of the wolfberry-astragalus compound polysaccharide. The wolfberry-astragalus compound polysaccharide is prepared by adopting a process of separately extracting wolfberry polysaccharide and astragalus polysaccharide and then mixing, the total sugar content is 70-80%, the uronic acid content is 10-14%, and the wolfberry-astragalus compound polysaccharide has specific molecular weight distribution and monosaccharide composition characteristics. Animal experiment results show that the wolfberry and astragalus compound polysaccharide can significantly improve peripheral hemopenia of myelosuppression mice induced by cyclophosphamide, promote recovery of the number of leukocytes, lymphocytes, monocytes and neutrophils, and effectively reverse weight loss caused by chemotherapeutic drugs. The wolfberry-astragalus compound polysaccharide provided by the invention is high in safety and remarkable in anti-myelosuppression effect, and can be used for preparing medicines or functional preparations for improving myelosuppression related diseases.