93results about How to "Avoid drug resistance" patented technology

The invention relates to lactobacillus crispatus LC30 for preventing and treating female genital tract infection and application of the lactobacillus crispatus LC30. The lactobacillus crispatus LC30 is preserved in the China General Microbiological Culture Collection Center with the preservation number of CGMCC NO.31923. The lactobacillus crispatus LC30 has good hydrogen peroxide production capacity and adhesion capacity, has a strong inhibition effect on main vaginitis pathogenic bacteria, and can be used for preventing and treating female genital tract infection. The composition is effective to nutritional pathogenic bacteria, and can also reduce form transformation and biological membrane formation of the pathogenic bacteria and prevent and treat drug resistance; besides, the fermentation product can reduce the content of pathogenic bacteria metabolites, regulate the secretion of cytokines and achieve the effect of relieving inflammation, so that the fermentation product can be used for preparing products for preventing and treating genital tract pathogenic bacteria infection, preventing and treating vaginitis and / or removing pudendum peculiar smell.

The invention discloses a single-cell drug causal gene discovery method and system based on adversarial transfer learning, and relates to the technical field of drug sensitivity prediction.The method comprises the steps that a source domain data set and a target domain data set are obtained, an adversarial transfer model is constructed, and the adversarial transfer model is trained and optimized according to the source domain data set and the target domain data set; obtaining a single-cell drug sensitivity prediction result; modeling single-cell gene expression data, combining a k-adjacent matching algorithm and a single-cell drug sensitivity prediction result to obtain an anti-fact value, and further calculating a hybrid effect and a difference effect; and calculating the causal effect based on the hybrid effect and the difference effect, and screening out the causal gene of the single-cell drug. On one hand, the key problem of lack of drug sensitivity labeling data in single cell analysis is solved, on the other hand, the causal gene is successfully found, and an important clue is provided for deep understanding of a drug action mechanism.

The invention discloses a vibration signal synthesis method for interfering the mating behavior of Liriomyza trifoliate, which can effectively trigger resonance on leaves and interfere natural vibration signals of Liriomyza trifoliate by playing artificially synthesized vibration signals with frequencies similar to those of Liriomyza trifoliate courtship signals, so that the communication between male and female is submerged, and the mating behavior of Liriomyza trifoliate is inhibited. The method has the advantages of preventing the pests from smoothly completing the mating process, utilizing the synthesis and transmission technology of the vibration signals, having remarkable advantages, not only effectively reducing the population reproductive capacity of the pests, but also avoiding the problems of environmental pollution and pest resistance of insecticides, and being a sustainable solution for agricultural pest prevention and control in the future.

The invention discloses a GSH response type cell membrane bionic nano-drug, a preparation method and application, the cell membrane bionic nano-drug comprises cell membrane vesicles serving as a carrier, the cell membrane vesicles are prepared by performing cell membrane extraction and membrane extrusion on immune cells of which the membrane surfaces overexpress azide, and the cell membrane vesicles serve as a carrier. The azide on the cell membrane vesicles and the bio-coupling compound are bonded through a click chemical reaction to form-SH groups, and the-SH groups and the small-molecule inhibitor are combined together in a disulfide bond mode through the cross-linking agent. The drug is connected with a small-molecule inhibitor through a disulfide bond, so that the drug loading rate is relatively higher, the stability is better, and drug leakage in the blood circulation process is relatively less; the small-molecule inhibitor can be completely released only in a tumor site microenvironment, so that the tumor m6A methylation level is reduced, the EMT state is reversed, the tumor drug resistance is overcome, and a better tumor treatment effect is displayed in cooperation with chemotherapy drugs for tumor treatment.

The invention discloses preparation and application of a core-shell structure carbon-coated copper nano-antibacterial agent with a slow release function, and relates to the technical field of antibacterial nano-metal materials, and the preparation method comprises the following steps: preparing by using a plasma arc method, taking pure copper as an anode, selecting a graphite rod as a cathode, and after the vacuum degree of an electric arc furnace reaches 10 <-4 > Pa or above, carrying out ultrasonic treatment; the method comprises the following steps: injecting a carbon source into a high-vacuum cavity, purifying in argon, collecting black powder consisting of nano-particles, carrying out a carbon shell burning process on the collected nano-particles, cooling, and recovering. The prepared carbon-coated copper nano-particles are of a core-shell structure with copper as a core and porous carbon as a shell, the porous carbon shell protects the nano-copper particles from being oxidized easily, the high activity of the nano-copper is kept, the nano-copper can achieve efficient and broad-spectrum sterilization at low concentration, meanwhile, slow release of the copper is achieved, and it is guaranteed that the antibacterial agent is higher in biological safety and environment friendliness.

The invention relates to combined lactobacillus salivarius LS100 for inhibiting vaginitis pathogenic bacteria and preventing and treating vaginitis and application of the combined lactobacillus salivarius LS100. The combined lactobacillus salivarius LS100 is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCC NO.32135. The combined lactobacillus salivarius LS100 has good hydrogen peroxide production capacity and adhesion capacity, and can be used for preparing medicines for preventing and treating vaginitis. The traditional Chinese medicine composition has a relatively strong inhibition effect on main vaginitis pathogenic bacteria, is effective on nutritional pathogenic bacteria, and can also reduce form transformation and biological membrane formation of the pathogenic bacteria and prevent generation of drug resistance; besides, the fermentation product can reduce the content of pathogenic bacteria metabolites, regulate the secretion of cell factors and play a role in relieving inflammation, so that the fermentation product can be used for preparing products for preventing and treating genital tract pathogenic bacteria infection and / or preventing and treating vaginitis.

The invention discloses a photodynamic antibacterial fiber membrane and a preparation method and application thereof, and relates to the field of functional materials.The preparation method comprises the steps that aggregation-induced emission photosensitive molecules are dissolved in a first solvent, and a photosensitive molecule solution is obtained; dissolving the purified polymer in a second solvent, and adding a photosensitive molecular solution to obtain a spinning precursor solution; adjusting and controlling the orientation degree of the spinning precursor solution through electrostatic spinning to obtain a fiber membrane; adjusting and controlling the crystallinity of the fiber membrane to obtain the photodynamic antibacterial fiber membrane. According to the preparation method provided by the invention, a polymer condensed state structure is combined with aggregation-induced emission photosensitive molecules, and a rigid confinement domain is provided for the photosensitive molecules by utilizing polymer crystallization and an orientation structure; meanwhile, the contact area between the material and oxygen is increased by utilizing the nanoscale of the electrostatic spinning fiber membrane, and the active oxygen yield of the material is synergistically improved.

The invention belongs to the technical field of ocean, and particularly relates to sea cucumber polysaccharide with an anti-colitis effect and application of the sea cucumber polysaccharide. According to the invention, the holothuria leucospilota polysaccharide H.leucospilota PS with a brand new structure is separated and purified from holothuria leucospilota, and the holothuria leucospilota polysaccharide H.leucospilota PS is composed of five monosaccharides, namely glucosamine, glucuronic acid, galactosamine, galactose and fucose. According to the present invention, by changing the intestinal microflora structure, the polysaccharide further regulates the generation of 3-Hydroxybutyrate, L-Meliic acid and Succinic semialdehydes, such that the intestinal energy metabolism is regulated, the intestinal microflora disorder and the energy metabolism disorder caused by the colitis are improved, and the drug resistance and the side effect caused by the long-term taking of the colitis treatment agent can be avoided;

The invention relates to combined lactobacillus salivarius LS360 for preventing and / or improving female genital tract infection and application of the combined lactobacillus salivarius LS360. The combined lactobacillus salivarius LS360 is preserved in the China General Microbiological Culture Collection Center (CGMCC), the preservation number is CGMCC NO.32134, and the combined lactobacillus salivarius LS360 has good biological characteristics and endurance capacity and can be used for preventing and / or improving female genital tract infection. The traditional Chinese medicine composition has a relatively strong inhibition effect on main vaginitis pathogenic bacteria, is effective on nutritional pathogenic bacteria, and can also reduce form transformation and biological membrane formation of the nutritional pathogenic bacteria and prevent and treat drug resistance; besides, the bacterial strain and the fermentation product can also reduce the content of pathogenic bacteria metabolites, regulate the secretion of cell factors and play a role in relieving inflammation, so that the bacterial strain and the fermentation product can be used for preparing products for preventing and treating female genital tract pathogenic bacteria infection, preventing and treating vaginitis and / or removing pudendum peculiar smell.

The application discloses an organic compound or a pharmaceutically acceptable salt thereof, and provides the organic compound or the pharmaceutically acceptable salt thereof and a pharmaceutical composition containing the same, wherein the organic compound is independently selected from the following compounds:

The invention belongs to the technical field of biology, and particularly relates to application of baicalin to preparation of a medicine for treating preeclampsia by inhibiting trophoblast ferroptosis induced by NETs. The baicalin inhibits downstream signal transduction by specifically interfering a key activation step of PKC [beta] 2 and a membrane translocation process coupled with a second signal, and finally plays a role in inhibiting ferroptosis of trophoblasts. The discovery provides a brand new perspective for understanding the molecular action mechanism of the medicine. According to the application, baicalin influences ferroptosis induced by NETs in trophoblast cells by inhibiting membrane translocation and activation of PKC beta2, so that the biological function of baicalin is exerted. The discovery of the mechanism further deepens the important significance of people on NETs and baicalin in disease induction and treatment, and a thought is provided for research and development of a new treatment scheme for preeclampsia.

The invention discloses a quaternary ammonium salt / magnetic biochar composite material and a preparation method and application thereof.The preparation method includes the steps that rice husk-based biochar is prepared from agricultural waste rice husks, magnetic iron-based biochar is synthesized through an alkaline chemical coprecipitation method, and then the quaternary ammonium salt / magnetic biochar composite material is prepared from the magnetic iron-based biochar and quaternary ammonium salt compounds through an ion exchange method; the obtained composite material has a remarkable sterilization effect and excellent quaternary ammonium salt slow-release performance; secondly, the used quaternary ammonium salt / magnetic biochar composite material can be used as a raw material to prepare the regenerated quaternary ammonium salt / magnetic biochar composite material, the sterilization effect is better than that of the first use, and the recycling of the composite material is realized. The preparation method is wide in raw material source and low in cost, the obtained composite material adsorbs free thalli through high-density charges, generation of drug resistance and secondary environmental pollution are avoided, the composite material can be applied to sewage and wastewater treatment, and a new material and a new method are provided for solving the pollution problem of pathogenic bacteria in water.

The invention discloses a preparation method of 6-phenyl-2, 3, 4, 5- The invention discloses a 2, 2 '-bipyridine platinum (II)-guanine N-heterocyclic carbene complex and a preparation method and application thereof, and particularly relates to the complex shown in the specification, the complex has dual inhibitory activity on tumor cells and tumor stem cells at the same time, the problem of platinum drug resistance caused by the tumor stem cells, glutathione and other proteins can be solved, and the drug resistance of the tumor cells and the glutathione can be improved. The compound can be used for preparing drugs for treating various malignant tumors, especially drugs for treating malignant tumors resistant to existing chemotherapeutic drugs. The preparation method is simple and easy to operate. In addition, the 6-phenyl-2, 2 '-bipyridine ligand disclosed by the invention contains carbon anions with relatively strong electron donating ability, and compared with a terpyridine ligand, the 6-phenyl-2, 2'-bipyridine ligand disclosed by the invention can further stabilize a metal ion center, so that the ability of resisting GSH interference is improved, and better stability is shown.

The invention belongs to the technical field of biological medicine, and relates to a kidney cancer axitinib drug-resistant cell model and a construction method and application thereof. The technical problems that in the prior art, a kidney cancer axitinib drug resistance mechanism is not clear, and a drug resistance target of the mechanism and a cell model of a molecular mechanism are lacked for research are solved. The invention provides a construction method of a kidney cancer axitinib drug-resistant cell model. The construction method comprises the following steps: constructing a nude mouse subcutaneous tumor-bearing model by using a kidney cancer cell line; when a subcutaneous tumor of a nude mouse reaches a target volume, after axitinib gavage treatment is performed on the nude mouse, first-generation drug-resistant kidney cancer cells are obtained through separation; shearing the tumor blocks into tumor blocks, inoculating the tumor blocks to the armpits of nude mice, and repeating the steps of gavage, separation and inoculation to respectively obtain second-generation and third-generation drug-resistant kidney cancer cells; and cutting the third-generation drug-resistant kidney cancer cells into pieces, digesting and culturing to obtain the kidney cancer axitinib drug-resistant cell model. The constructed kidney cancer axitinib drug-resistant cell model is applied to research of a kidney cancer cell targeted therapy drug-resistant mechanism and screening or preparation of kidney cancer targeted drugs.

The application discloses a method for treating hydrogen sulfide in oil wells, which comprises the following steps: (1) screening of test oil reservoir; (2) preparation of specific virulent SRB phage stock solution; (3) enrichment of specific virulent SRB phage; (4) determination of optimal titer of specific virulent SRB phage solution; (5) selection of phage fusion bactericide; (6) determination of optimal intervention point of bactericide; (7) field test and effect evaluation. The application has the following advantages: (1) wide adaptability, simple method and good operability; (2) strong pertinence; (3) phage bactericidal action can prevent the generation of bacterial drug resistance and can prolong the use time of bactericide; (4) the cost is reduced by more than 50% compared with the prior art; meanwhile, the damage of bactericide toxicity to the formation is reduced, and the method is economic and environment-friendly.

The invention relates to the field of new application effects of bacterial strains, in particular to application of bacillus subtilis metabolite-iturin in relieving drug resistance of silver nanoparticles, the bacillus subtilis metabolite-iturin is prepared into the iturin-silver nanoparticles (Ituin-AgNPs), the preparation method comprises the steps of ultraviolet irradiation, then centrifugation, supernatant discarding, precipitate collection and finally freeze drying, the particle size is 21 + / -7 nm, and the Ituin-AgNPs is prepared into the iturin-silver nanoparticles (Ituin-AgNPs). The content of the silver element is 145 mu g / mg. The invention discloses application of iturin in removing the drug resistance of silver nanoparticles, drug resistance is not generated in the using process, related genes and proteins for determining the drug resistance of escherichia coli are determined, the interaction mechanism of ItuinA-AgNPs and escherichia coli is clarified, the practical application potential of ItuinA-AgNPs is fundamentally mastered, and the application prospect is wide. And a new thought is provided for preventing drug resistance of bacteria to the silver nanoparticles.

The invention provides an anti-allergy soothing non-allergy surface layer material, a preparation method thereof and a sanitary towel prepared from the anti-allergy soothing non-allergy surface layer material, and belongs to the technical field of sanitary towels. Nostoc commune and nostoc sphaeroids kutz are subjected to enzymolysis separation to prepare algae antibacterial peptide, the algae antibacterial peptide is connected with cordycepin, chelated with Cu ions and added into a chitosan solution together with probiotic microspheres and a purslane flavone extract in a stirring mode, temperature-sensitive hydrogel is prepared and evenly sprayed on cotton cloth, and the anti-allergy soothing non-sensitive surface layer material is prepared. The prepared anti-allergy soothing non-allergy surface layer material does not contain chemical allergens, the allergy risk is inhibited from the source, the whole production link is produced in a sterile and dust-free purified environment, no sensitization is achieved, the material is more skin-friendly, and meanwhile the material has multiple biological functions of resisting bacteria and inflammation, regulating and controlling ecological balance of private parts and the like, so that skin itch is relieved, skin is relieved, allergy is resisted, and the anti-allergy soothing non-allergy surface layer material has a good application prospect. The health-care product has the effects of protecting skin barrier, resisting inflammation and the like, also can reduce colpitis and the like, is good in skin friendliness, protects the private health of women in a menstrual period, and is more comfortable to use.

The invention belongs to the technical field of gene engineering, and provides a small interfering RNA for silencing SAP102. The small interfering RNA is si-RNA-1 of which the sequence is SEQ ID NO: 1 and / or si-RNA-2 of which the sequence is SEQ ID NO: 2. The small interfering RNA for silencing the SAP102 can significantly inhibit the activity of breast cancer cells and avoid the generation of drug resistance, and provides a new potential scheme for clinical tumor treatment.

The invention discloses application of an MYRF gene in preparation of a composition for treating glioma, overexpression of the MYRF gene in glioma cells enables the glioma cells to be transdifferentiated into glial cells, MBP and MAG signals are up-regulated, and PDGFR alpha signals are down-regulated. The invention also provides an application of the MYRF gene in preparation of a glioma cell transdifferentiation reagent, and a method and a composition for transdifferentiating glioma cells into glioma cells. The invention has great significance on treatment of glioma, provides a new strategy for treatment of glioma, possibly contributes to reducing the malignant degree of tumor and overcoming the drug resistance of tumor cells, and possibly improves the effect of immunotherapy by changing the tumor microenvironment. In addition, the invention also helps to deeply understand the pathological mechanism of glioma, and provides a theoretical basis for developing new therapeutic targets and drugs.

This invention belongs to the field of pharmaceutical technology for the treatment and prevention of oral diseases, and particularly relates to a porphyrin and ferrocene derivative, nanoparticles, preparation methods, and applications of synergistic photothermal and acoustic therapy for oral diseases. The porphyrin and ferrocene derivative are formed by organically combining tetraaminophenylporphyrin and diformaldehyde ferrocene through imine dynamic covalent bonds. This invention, through innovative raw material coupling methods and functional integration design, synergistically achieves PTT-SDT dual therapy, providing a new and superior technical solution for precise, efficient, and low-drug-resistance treatment of deep oral bacterial-fungal biofilm infections.

The invention relates to a multi-dimensional synergistic acne removal composition as well as a preparation method and application thereof, and belongs to the technical field of cosmetics. The invention provides a multi-dimensional synergistic acne removal composition. The multi-dimensional synergistic acne removal composition is prepared from Shangjiang, hydrolyzed sericin, a lactobacillus / pomegranate fruit fermentation product extract, a white willow bark fermentation product and quaternary ammonium salt-73. According to the invention, Shangjiang, hydrolyzed sericin, lactobacillus / pomegranate fruit fermentation product extract, white willow bark fermentation product and quaternary ammonium salt-73 are scientifically compounded to construct a unique'prevention-treatment-repair 'three-in-one synergistic network; the five components respectively aim at four key paths of acne morbidity, namely bacterial proliferation, abnormal hair follicle keratinization, excessive sebum secretion and inflammatory response, full-path coverage from pathogenesis blocking to symptom relieving to barrier repairing is realized, and the synergistic acne removal effect is obviously superior to that of a traditional acne removal product only aiming at a single link.

The invention provides a marker for detecting gastric cancer immunotherapy drug resistance, application of the marker, a model and a use method of the model, provides remarkable influence of a low-oxygen tumor microenvironment on gastric cancer immunotherapy drug resistance, and highlights key effects of FOXO3a and PD-L1 expression in a mediated immune escape mechanism. An innovative microfluidic tumor chip model is adopted, the in-vivo tumor hypoxia environment is successfully simulated, and how FOXO3a up-regulation induced by the hypoxia condition causes PD-L1 expression enhancement and further causes immunotherapy drug resistance is disclosed.

The invention relates to an ultrasonic response type copper molybdate nano material as well as a preparation method and application thereof. The chemical formula of the nano material is HCu1. 5MoO5. The preparation method comprises the following steps: mixing a molybdenum source, urea and a copper source in water, cooling, crystallizing, and carrying out ultrasonic treatment. Compared with the prior art, the nano material can synchronously generate active oxygen and controllably release copper ions at a tumor site under the accurate control of exogenous ultrasound, and efficiently subvert the copper steady state in tumor cells through the biaxial synergistic effect of'efflux inhibition 'and'internal supply enhancement', so that copper death is specifically induced, and the tumor cell apoptosis is inhibited. And anti-tumor immune response is synergistically activated, so that effective application in treatment of bladder cancer by inducing copper death, especially in treatment of malignant solid tumors such as bladder cancer and pancreatic cancer, is realized.

The application provides a camellia plant endophytic bacteria, a bacterial agent thereof and an anti-influenza virus A application; the camellia plant endophytic bacteria is Bacillus thuringiensis, which is preserved in the China General Microbiological Culture Collection Center with a preservation number of CGMCC No.32945. The fermentation product of the strain shows good antiviral activity in in vitro and in vivo experiments, can effectively inhibit the replication of influenza virus, and protect infected mice from lethal influenza virus; also shows the effect of regulating immune response, enhances the secretion of IFN-gamma by down-regulating the secretion of pro-inflammatory factors such as IL-6 and IL-10, thereby improving the immune resistance of the body.

The invention relates to an application of osimertinib combined with chidamide in treatment of osimertinib drug-resistant lung cancer, and particularly relates to the following aspects: (1) osimertinib is an epidermal growth factor receptor tyrosine kinase inhibitor (EGFR-TKI), and shows a remarkable curative effect in treatment of non-small cell lung cancer (NSCLC) patients carrying specific EGFR mutation; (2) chidamide is a novel oral HDACi, can induce cell apoptosis, cell cycle arrest and cell growth inhibition, and shows good tolerance and antitumor activity in various cancer types; and (3) chidamide is taken as an epigenetic regulating agent and can be in synergistic effect with osimertinib, so that the capability of killing lung cancer cells is enhanced. The invention belongs to the technical field of medicines, and particularly provides application of osimertinib combined with chidamide in treatment of osimertinib drug-resistant lung cancer.

The present application relates to a kind of copper-doped tricobalt tetraoxide (Cu-Co3O4) nanometer antibacterial agent and its application in the preparation of keratitis treatment product, belong to the field of biotechnology.The present application uses cobalt nitrate hexahydrate and copper nitrate trihydrate as raw material, uses isopropyl alcohol and glycerol as solvent, and successfully prepares copper-doped tricobalt tetraoxide nanometer antibacterial agent by solvothermal method.Experiment shows that under the near-infrared laser irradiation of wavelength 808nm, power density 0.5W / cm 2 , copper-doped tricobalt tetraoxide nanometer antibacterial agent has good photo-thermal, photodynamic performance and broad-spectrum antibacterial activity.In vitro experiment verifies that it can efficiently kill pathogenic bacteria, and is not prone to cause drug resistance;In vivo experiment verifies that it can effectively treat MRSA infected bacterial keratitis.The present application lays a theoretical foundation for the preparation of keratitis treatment, effective nanometer antibacterial agent and drug.

The application discloses application of a CDK4 / 6 inhibitor combined with oxaliplatin in preparation of a treatment drug for chemotherapy-resistant colorectal cancer. The combined use of the CDK4 / 6 inhibitor combined with oxaliplatin has excellent therapeutic effect on locally advanced rectal cancer which is resistant to a first-line chemotherapy scheme, can overcome the drug resistance of the rectal cancer patient to the chemotherapy drug, so that the tumor proliferation is inhibited, and the apoptosis of the cancer cell is promoted. The application provides a combined drug treatment scheme for neoadjuvant therapy of locally advanced rectal cancer chemotherapy resistance, can achieve the purpose of effectively treating or assisting in treating the rectal cancer, and provides a new way for treating, diagnosing or predicting the rectal cancer patient.

The present invention discloses an intelligent device for integrated physical and chemical multimodal diagnosis and treatment of chronic wounds, which relates to the technical field of flexible intelligent bandage devices, including an intelligent detection system, a treatment system, a flexible spinning substrate and a flexible circuit board. The intelligent detection system and the treatment system are integrated on the flexible spinning substrate. The present invention takes multiple angles related to chronic wounds, including physical and chemical signals, and integrates impedance sensing, temperature sensing, pH sensing, uric acid sensing, glucose sensing and other detection means to obtain chronic wound healing information, comprehensively judge the inflammation of the wound, and adopt an electrical stimulation treatment method. Extremely low voltage and current can eliminate harm to the human body, and cell proliferation, migration and differentiation can be promoted by regulating the endogenous electric field to achieve rapid healing. This method based on the mechanism of human wound healing avoids drug resistance and other toxicity problems and is relatively safer.

The present invention relates to a mercaptophenol derivative having a structure shown in formula (I) or a pharmaceutically acceptable salt thereof or a stereoisomer thereof, and use thereof in the preparation of an antibacterial drug. The mercaptophenol derivative of the present invention has many advantages such as a broad antibacterial spectrum, good biocompatibility, low cytotoxicity (high efficiency and low toxicity), a novel mechanism of action, a fast sterilization speed, and low drug resistance.

The invention belongs to the technical field of biological medicine, and discloses application of isoliquiritigenin as a polymyxin synergist. Through combination of isoliquiritigenin and polymyxin, it is found that isoliquiritigenin and polymyxin have a good effect on in-vivo and in-vitro salmonella inhibition. The isoliquiritigenin is safe in component, can achieve a good synergistic effect with the polymyxin, can significantly reduce the dosage of the polymyxin, is not easy to induce the generation of bacterial drug resistance, and has the characteristics of low price and good curative effect.