395 results about "Glycosidic bond" patented technology

The invention discloses application of fucoidin degrading enzyme OUC-MgFucD1 in preparation of fucoidin oligosaccharide, and belongs to the technical field of preparation of the fucoidin oligosaccharide. The amino acid sequence of the fucoidin degrading enzyme OUC-MgFucD1 is as shown in SEQ ID NO. 1, and the fucoidin degrading enzyme OUC-MgFucD1 is as shown The fucose oligosaccharide is fucose, fucodisaccharide and fucotriose, the molecular formula of the fucose is C6H12O8S, the molecular formula of the fucodisaccharide is C12H22O15S2, and the molecular formula of the fucotriose is C18H32O16S. The invention further discloses a preparation method of the fucose oligosaccharide. The fucoidin degrading enzyme OUC-MgFucD1 disclosed by the invention can specifically act on fucoidin, and a specific fucoidin oligosaccharide combination is generated by precisely cutting a main chain glucosidic bond of the fucoidin. The invention provides a key technical support for the development of high-added-value fucoidan oligosaccharide products, and has important significance for promoting the industrial application of the fucoidan oligosaccharide in the fields of functional foods, biological medicines and the like.

To provide beta-1,3-glucan, and to provide uses thereof for regulating immunity in the human body, which are methods for treating and / or preventing high cholesterol, diabetes, and allergies, and methods for treating and / or preventing intestinal inflammation.SOLUTION: Provided is a composition for oral administration for alleviating symptoms associated with intestinal inflammation in humans, the composition containing an effective amount of beta-1,3-glucan dried to a moisture content of about 40% or less from Euglena grown by fermentation, the beta-1,3-glucan not containing beta-1,6-glycosidic bonds, the effective amount being between 0.1 mg of beta-1,3-glucan / kg body weight and 100 mg of beta-1,3-glucan / kg body weight.SELECTED DRAWING: Figure 1

The invention belongs to the field of natural product extraction, and particularly relates to a method for extracting chitosan by using hermetia illucens in a high-valued manner. According to the method, the ionic liquid is introduced while the chitosan is extracted by adopting a deep eutectic solvent method, the deep eutectic solvent can form strong interaction with chitin molecules, the ionic liquid can further enhance specific recognition of the chitin molecules, and the ionic liquid and the deep eutectic solvent have a synergistic effect, so that solubility and recognizability of the chitin are improved; protein and salt are efficiently removed, meanwhile, glucosidic bonds of a chitin macromolecular main chain are prevented from being hydrolyzed and broken, and the extraction rate and purity of chitosan are remarkably improved. According to the method, the extraction condition is mild, the eutectic solvent can be recycled and reused, the extraction process flow of the chitosan is simplified, and the method is environment-friendly.

The invention discloses astragalus polysaccharide processed by different processing technologies and application thereof, self-weight polysaccharides of RAPSs, SAPSs, WAPSs and HAPSs are extracted from crude products and processed products obtained by salt processing, wine processing and honey processing, and the influence of different processing methods on the structure and bidirectional immunomodulatory activity of the astragalus polysaccharide is discussed. Results show that different processing methods change the properties and structural characteristics of the astragalus polysaccharide so as to influence the bidirectional immunomodulatory activity of the astragalus polysaccharide; the extraction rate, chemical composition, molecular weight, monosaccharide composition, chain conformation and surface morphology, Zeta potential and particle size, immunity enhancement and anti-inflammatory activity and the like of the four polysaccharides are remarkably different, but the four polysaccharides have the same typical crystal interior, main glycoside residues and O-glucosidic bonds. In a test concentration range, the honey-processed polysaccharide has a relatively strong immunity enhancing capability, and the wine-processed polysaccharide has a relatively strong anti-inflammatory capability.

The invention discloses a method for efficient conversion and utilization of mogroside. According to the method, mogroside V is converted through fermentation of candida parapsilosis L7, and siamenoside I is directionally synthesized. According to the invention, Candida parapsiloides L7 is utilized for fermentation conversion, and beta-1, 6-glucosidic bonds in glucose branched chains connected with C3-site hydroxyl of a mogroside V structure parent nucleus can be directionally hydrolyzed with high regioselectivity (the beta-1, 6-glucosidic bonds on glucose branched chains connected with C24-site hydroxyl are hardly hydrolyzed), so that one molecule of glucose is removed, and siamenoside I is obtained. The siamenoside I provided by the invention can be efficiently prepared, and the maximum molar yield of the siamenoside I can reach 87.40%. The siamenoside I has more obvious selective hydrolysis characteristic, higher space time yield of the siamenoside I, no cytotoxicity of the siamenoside I and better anti-inflammatory and antioxidant activity, provides a new drug choice for diseases such as inflammation and the like, and provides technical support for development of high-valued products of mogroside.

The invention relates to beta-1, 4-glucanase and application thereof, and belongs to the technical field of biology. A novel beta-1, 4-glucanase is screened and identified in Pleurotus tuber-regium, and the beta-1, 4-glucanase has wide substrate specificity, can specifically hydrolyze beta-glucan (such as sodium carboxymethyl cellulose) containing beta-1, 4 glucosidic bonds, and can hydrolyze beta-glucan (such as sodium carboxymethyl cellulose) containing beta-1, 3-1, 4-glucanase) containing beta-1, 3-1, 4-glucanase. The beta-1, 4-glucanase disclosed by the invention can be used for preparing beta-glucan (such as barley beta-glucan and oat beta-glucan) containing beta-1, 3-1, 4 glucosidic bonds, so that the beta-1, 4-glucanase disclosed by the invention has good enzyme activity on the beta-glucan containing the beta-1, 4 glucosidic bonds and the beta-glucan containing the beta-1, 3-1, 4 glucosidic bonds. Besides, the enzyme activity of the beta-1, 4-glucanase disclosed by the invention can be kept at 70% or above under the condition that the pH value is 4.0-7.0, the enzyme activity of the beta-1, 4-glucanase can be kept at 88% or above under the temperature of 40 DEG C, and the beta-1, 4-glucanase has good stability.

The invention relates to the technical field of plant polysaccharide extraction, in particular to a process for synergistically extracting rape pollen polysaccharide by a compound enzyme, which comprises the following steps: crushing rape pollen to 200-300 meshes by pulse airflow, performing microwave-ultrasonic degreasing by using a 85% ethanol solution, mixing with an acetic acid-sodium acetate buffer solution with the pH value of 4.8, and performing ultrasonic extraction to obtain a crude extract; the method comprises the following steps: adding a compound enzyme system which is immobilized by Fe3O4 (at) SiO2 nanoparticles by cellulase, pectinase, protease and beta-glucosidase according to a mass ratio of 2: 1: 1: 0.5, carrying out enzymolysis for 4 hours in a 50 DEG C constant-temperature water bath and a 15V / cm pulsed electric field, carrying out enzyme deactivation on enzymatic hydrolysate, carrying out gradient ethanol precipitation, carrying out ultrafiltration deproteinization, and carrying out vacuum pulsation freeze drying to obtain the polysaccharide. According to the method disclosed by the invention, the cell wall breakage rate and the enzymolysis efficiency are improved through pulsed airflow crushing, magnetic nano immobilized enzyme and pulsed electric field assistance, the polysaccharide yield reaches 7.32%, the purity is 91%, the beta-glucosidic bond proportion and the antioxidant activity are remarkably improved, the enzyme can be recycled and reused, the drying time is shortened, and efficient and low-consumption extraction is realized.

The invention relates to polystyrene cation exchange resin as well as a preparation method and application thereof. The preparation method comprises the following steps: dissolving a dispersing agent in a solvent to obtain a dispersed phase for suspension polymerization reaction, uniformly mixing a reaction monomer, a cross-linking agent, a pore-foaming agent and an initiator, and carrying out suspension polymerization reaction under segmented stirring and segmented temperature change to prepare copolymer microspheres; and putting the dried microspheres into a reaction kettle, swelling, carrying out sulfonation reaction through a variable temperature program on the basis to obtain sulfonated microspheres, and carrying out alkali exchange reaction on the sulfonated microspheres to obtain the final product cation exchange resin. Compared with the prior art, the cation exchange resin preparation method provided by the invention has the characteristics of simple and feasible process, easiness in large-scale production, low cost, high specific surface area, high adsorption capacity and high separation efficiency, and is especially suitable for separating and purifying substances containing amino groups or aminoglycosidic bonds.

The invention provides agar oligosaccharide with an antioxidant function as well as a preparation method and application of the agar oligosaccharide, and belongs to the technical field of degradation of natural polysaccharides. According to the method, a H2O2 / Fe < 2 + > system is adopted to generate hydroxyl free radicals to degrade agar polysaccharide, glycosidic bonds in the agar polysaccharide are oxidized and broken by H2O2 under the catalysis of Fe < 2 + >, so that the agar polysaccharide is degraded into low-molecular fragments, agar oligosaccharide with relatively good antioxidant activity is obtained, then the agar oligosaccharide is compounded with vitamin C, and the antioxidant activity of the agar oligosaccharide is improved. The color of the freeze-dried and redissolved agar oligosaccharide solution can be reduced, and meanwhile, the degraded agar oligosaccharide can be endowed with stronger antioxidant activity. The preparation method provided by the invention is simple in process flow, low in equipment requirement, low in cost and high in agar oligosaccharide yield which can reach 85% or above, and is suitable for industrial large-scale popularization.

Disclosed herein are compositions comprising insoluble alpha-glucan particles having a high degree of crystallinity and small particle size. For example, the alpha-glucan particles can have a degree of crystallinity of at least about 0.65, and / or an average size of less than a micron. At least 50% of the glycosidic linkages of the insoluble alpha-glucan in the disclosed particles are alpha-1,3 glycosidic linkages. Further disclosed are methods of producing insoluble alpha-glucan particles, as well as their use in various applications and products.

The invention relates to a preparation method of high-substitution-degree cationic micro-nano cellulose and application of the high-substitution-degree cationic micro-nano cellulose to improvement of water filtering performance of paper. In the enzymolysis pretreatment process, beta-1, 4 glucosidic bonds among glucose molecules are cut off, and more reaction binding sites are created for cationization after cellulose in an amorphous region and a part of crystalline region is hydrolyzed, so that the use amount of chemical reagents can be effectively reduced, and the environmental pollution is reduced. The prepared high-substitution-degree cationic micro-nano cellulose (CMFC) has abundant positive charges, weakens the hydrogen-bond interaction of cellulose molecules, improves the content of nano-fibrillation, and effectively reduces the mechanical preparation energy consumption of the CMFC. CMFC pulp is added into paper pulp fibers, so that pores of the paper fibers are blocked, more filler is intercepted, and the filler is difficult to be completely brought into white water by water flow in the water filtering process, so that the retention rate is increased, and the water filtering performance is effectively improved.

The present invention provides a novel pressure-sensitive adhesive composition including a glucan derivative. The pressure-sensitive adhesive composition of the present invention includes a glucan derivative G having a glucoside bond A, and a glucoside bond B having a different bonding form from the glucoside bond A. The glucoside bond A is, for example, a 1,6-glucoside bond. A pressure-sensitive adhesive sheet 1 of the present invention is formed from the pressure-sensitive adhesive composition. A laminate 10 of the present invention includes the pressure-sensitive adhesive sheet 1 and a substrate sheet 2.

The invention discloses a method for preparing highly branched starch by using double enzymes, and belongs to the field of biologically modified starch. According to the invention, glycogen branching enzyme and cyclodextrin glucosyltransferase are adopted to synergistically modify different crystal forms of starch in a non-gelatinization swelling system (60 DEG C) to prepare highly branched starch, the reaction is carried out on the premise of ensuring the morphology of starch particles, and the later processing application characteristics are ensured, so that the highly branched starch can be fused with a traditional starch staple food carrier, and the preparation process is simple and convenient. According to the method, the enzyme modified starch and the swelling reaction are synchronously carried out, the step of cooling after high-temperature gelatinization is reduced, the production efficiency is improved, the adverse effect of high viscosity caused by high-temperature gelatinization on starch products is avoided, and the obtained product is high in yield, high in branching degree, low in digestibility and high in glucosidic bond proportion; a healthy and green new thought is provided for the field of starch deep processing.

The invention discloses a method for improving moso bamboo cellulase hydrolysis conversion efficiency through amino acid-based deep eutectic solvent pretreatment, and belongs to the technical field of biomass recycling. The method comprises the following steps: mixing an L-cysteine solid and a formic acid solution, performing water bath heating to prepare an acidic eutectic solvent, performing microwave-assisted eutectic solvent pretreatment on moso bamboos, and performing filtration separation and enzymolysis; wherein the molar ratio of the L-cysteine to the formic acid is 1: (4-5). A deep eutectic solvent formed by formic acid and cysteine has a strong hydrogen bond network and an acid environment, beta-O-4 ether bonds in lignin are promoted to be broken, sulfydryl of cysteine participates in nucleophilic attack, a lignin-carbohydrate complex is further destroyed, formic acid can hydrolyze acetyl and glucosidic bonds in hemicellulose, and the lignin-carbohydrate complex is further destroyed. And degrading into an oligomer or monosaccharide. The phyllostachys pubescens components are efficiently separated, the cellulose enzymolysis saccharification capacity is improved, and effective utilization of all the phyllostachys pubescens components is facilitated.

The invention discloses hyaluronic acid endo-lyase and application thereof in preparation of hyaluronic acid with specific molecular weight. The hyaluronate lyase can cut a beta1-4 glucosidic bond to generate unsaturated oligosaccharide, has the characteristics of high enzyme activity and catalytic efficiency and good stability, and the enzymolysis reaction can be carried out in water without adding metal ions and a buffer solution, so that the hyaluronate lyase has the advantages of simplicity in product recovery and no need of removing salt. The hyaluronate lyase not only can be used for degrading hyaluronic acid, but also can be used for degrading chondroitin sulfate, not only can be used for preparing polysaccharide with specific molecular weight, but also can be used for completely degrading the polysaccharide into unsaturated disaccharide, and has a wide application prospect in the fields of cosmetics, food, health care products, medicines and the like.

The invention belongs to the technical field of chitosan oligosaccharide preparation, and particularly relates to a method for preparing chitosan oligosaccharide based on compound enzyme. According to the method for preparing the chitosan oligosaccharide based on the compound enzyme, a multi-enzyme synergistic system is matched with the design of a specific protective agent, chitosanase and chitinase can cooperatively cut beta-1, 4 glycosidic bonds of chitosan and control molecular weight distribution of products, meanwhile, chitinase can directionally cut the beta-1, 4 bonds at the tail end of chitosan, and the molecular weight distribution of the products is controlled. The papain is used for removing protein impurities in the chitosan to realize accurate control of the polymerization degree. Besides, a specific protective agent matched with the multi-enzyme synergistic system is specifically designed, the specific protective agent and the multi-enzyme composition act together, the molecular weight distribution width can be remarkably reduced, the yield and the DP 4-8 proportion can be increased, meanwhile, physical field ultrasonic assistance is added in the enzymolysis treatment process, and the enzymolysis treatment efficiency is improved. The combination of the substrate and the enzyme is further promoted, and the reaction time is shortened.

The invention discloses purified barley seedling polysaccharide as well as a preparation method and application thereof. The polysaccharide is prepared from six monosaccharides, namely rhamnose, galacturonic acid, glucose, galactose, xylose and arabinose in a molar ratio of 2.88: 3.39: 1.51: 1: 0.76: 3.2. The relative molecular weight is 254.09 kDa. A fermentation method is combined with an innovative purification process, so that the barley seedling polysaccharide monomer with high purity and uniform structure is obtained for the first time. Through a multi-dimensional characterization technology, fine structure characteristics such as monosaccharide composition, glucosidic bond connection mode and branching degree are systematically analyzed, and a reliable material basis is provided for establishing structure-function accurate association. The purified barley seedling polysaccharide has a remarkable effect of promoting intestinal peristalsis, not only lays a scientific foundation for deep processing of barley seedlings and research and development of related functional products, but also provides a new theoretical support for relieving intestinal peristalsis disorder, and has a wide application prospect.

The invention discloses faint scent pure rice vinegar and a preparation method thereof. The preparation method comprises the following steps: S1, raw material treatment, S2, liquefaction and saccharification, S3, alcoholic fermentation, S4, rice wine treatment, S5, acetic fermentation, S6, filtration, S7, nanofiltration, S8, sterilization, filling and the like. According to the present invention, starch debranching enzyme is adopted to hydrolyze alpha-1, 6-glucosidic bonds, 20-28 DEG C micro-low temperature prolonged time fermentation is adopted, precipitation distillation, ultrafiltration and nanofiltration double filtration and other processes are adopted, full fermentation, full decoloration and odor removal are performed, high-quality and odor-free rice vinegar is produced, the fermentation speed is rapid, the fermentation is complete, the preparation method is simple, the operation is convenient, the cost is low, and the method is suitable for industrial production. The method is high in additional value and has good popularization and application value.

The invention relates to the technical field of microbial engineering, and particularly discloses a compound microbial agent for treating cyanobacterial bloom, bacillus velezensis provided by the invention destroys algae cells and inhibits the activity of related biological enzymes by secreting heterocyclic algal killing active substances, and simultaneously causes the leakage of cellular contents (such as algal toxins, polysaccharides and lipids); an extracellular enzyme system (such as alkaline phosphatase and beta-glucosidase) of the sphingomonas decomposes free algal toxins into low-toxicity linear peptide fragments and hydrolyzes beta-1, 4-glucosidic bonds in EPS at the same time, so that algae-derived organic fragments are converted into small-molecular soluble substances, and a substrate is provided for follow-up metabolism of rhodococcus; lipase and esterase secreted by rhodococcus can hydrolyze triglyceride and waxy components, fatty acid is converted into energy and a biosurfactant through a beta-oxidation pathway, and hydrophilicity of frond fragments is enhanced to promote overall degradation efficiency.

The invention discloses an alpha-1, 4 glucosidic bond hydrolytic ability enhanced oligomerized 1, 6-glucosidase mutant and application thereof, and belongs to the technical field of gene engineering and enzyme engineering. The oligomerized 1, 6-glucosidase mutant V219A / P227A disclosed by the invention also has the alpha-1, 4 glucosidic bond hydrolytic activity which is not possessed by a wild type enzyme on the basis of retaining the original alpha-1, 6 glucosidic bond hydrolytic activity, and the alpha-1, 4 glucosidic bond hydrolytic activity is improved by 267% compared with that of a single mutant V219A. The mutant V219A / P227A is applied to an enzymatic production process of glucose, maltodextrin is taken as a substrate, glucamylase and the mutant V219A / P227A are added for enzymatic conversion, and the final glucose yield reaches 98.0%; the mutant V219A / P227A is applied to regeneration of glucose from crystalline glucose mother liquor, and the final glucose yield reaches 95.9%, which is 9.35% and 5.15% higher than those of a wild type V219A and a single mutant V219A respectively. Therefore, the mutant is helpful for improving the substrate conversion rate and the product purity in the glucose production process, can be used for regenerating glucose from crystalline glucose mother liquor, and has higher industrial application value.

To provide a technique capable of effectively recycling each recovered material as a single material by selectively decomposing, separating and recovering a fiber having a β - glycoside bond from a mixed fiber comprising the fiber having the β - glycoside bond such as cotton and other fibers such as polyester by a simple treatment in a short time under a non-pressurized condition.SOLUTION: A treatment method comprising selectively decomposing a fiber having a β - glycosidic bond from a mixed fiber containing the fiber having a β - glycosidic bond and a fiber composed of another material by a treatment using a deep eutectic solvent (DES) formed by mixing a hydrogen bond donor with a hydrogen bond acceptor at a molar ratio of 0.50 or more and 6.00 or less, and recovering the fiber composed of another material in a fibrous form.SELECTED DRAWING: None

The invention relates to a beta-glucan composition and application thereof. Specifically, the invention provides an application of a beta-glucan composition in preparation of a composition for improving or treating immune-related diseases, the beta-glucan is beta-glucan of which the molecule contains beta-1, 3 and beta-1, 6 glucoside bonds, and the molecular weight of the beta-glucan is 1-50kDa. The beta-glucan has the advantages of being novel in structure, controllable in quality, rich in source, simple in preparation process, high in product purity, high in biological activity, easy to industrially produce and the like, and the obtained water-soluble beta-glucan composition has better anti-tumor activity and can be used for preparing medicines. The compound is expected to be developed into a novel safe and effective medicament for improving or treating immune-related diseases and resisting tumors.

The invention relates to the technical field of skin care products, in particular to a glucan product as well as a preparation method and application thereof. According to the invention, the glucan product is obtained by performing enzymolysis on the material containing the glucan through the incision enzyme for specifically treating the 1, 4 glucosidic bonds, and the number ratio of the 1, 4 glucosidic bonds to the 1, 3 glucosidic bonds in the glucan product is 1.8 or less, so that the intramolecular and intermolecular agglomeration of the glucan is improved, the room-temperature stability of the glucan is improved, and the yield of the glucan is increased. And the obtained glucan product can be added in cosmetics in a large amount, has a stronger anti-inflammatory effect, and has an outstanding effect of promoting skin soothing after being added into the cosmetics.

The invention relates to a method for high-yield preparation of an icodextrin bulk drug by a single enzyme system, which comprises the following steps: gelatinizing starch at high temperature, adding alpha-thermoamylase for hydrolysis, carrying out ultrafiltration separation on primary enzymatic hydrolysate to obtain high-molecular-weight dextrin and alpha-thermoamylase, returning the high-molecular-weight dextrin and the alpha-thermoamylase to a gelatinization tank for secondary enzymolysis, and combining ultrafiltration permeates of the secondary enzymatic hydrolysate and the primary enzymatic hydrolysate, and performing molecular weight screening by ultrafiltration to obtain icodextrin of which the weight-average molecular weight and the number-average molecular weight simultaneously meet the requirements of alpha-1, 6 glucosidic bonds. On the basis of a traditional icodextrin manufacturing process, the working procedures of recovering macromolecular dextrin and performing secondary enzymolysis are added, and the yield can reach 85-91%. Particularly, new amylase does not need to be additionally added in the secondary enzymolysis, the original amylase is continuously used, new equipment is not added, the production cost increase is small, and the preparation efficiency is high.

The present disclosure relates to poly alpha-1,6-glucan ether compounds comprising poly alpha-1,6-glucan substituted with at least one positively charged organic group and having a degree of substitution of about 0.001 to about 3.0. The poly alpha-1,6-glucan comprises a backbone of glucose monomer units, wherein greater than or equal to 40% of the glucose monomer units are linked via alpha-1,6 glycosidic bonds, and optionally about 3% of the backbone glucose monomer units have branches via alpha-1,2 and / or alpha-1,3 glycosidic bonds. Compositions comprising poly alpha-1,6-glucan ether compounds can be used in various applications.

The invention discloses high-purity resistant dextrin and a preparation method thereof, and the preparation method comprises the following steps: (1) adding 4, 3-alpha-glucosyltransferase into a maltodextrin aqueous solution or a starch solution treated by starch debranching enzyme to react, and after the reaction is finished, deactivating the enzyme; (2) adding alpha-amylase into a reactant in the step (1) to react, and deactivating the enzyme after the reaction is finished; (3) adding alpha-glucosidase into the reactant in the step (2) for reaction, and deactivating the enzyme after the reaction is finished; (4) adding saccharifying enzyme into the reactant in the step (3) to react, and deactivating the enzyme after the reaction is finished; and (5) adding yeast powder into the reactant in the step (4) for fermentation, filtering by a filter membrane after fermentation, collecting membrane filtrate, and drying to obtain the high-purity resistant dextrin. The preparation process is simple and efficient, the product purity is high, the proportion of alpha-1, 3 glucosidic bonds in the prepared resistant dextrin is 40% or above, and the purity of the resistant dextrin is 80-90%.

This invention discloses a method for determining the content of suberin monomers in the suberized tissue of yam tubers. Suberin is a macromolecular polyester, with monomers mainly covalently linked by oxygen-ester bonds. Its aliphatic compounds are linked to the polysaccharide components in the cell wall by glycosidic bonds and located on the inner side of the cell wall. Therefore, to determine the suberin monomer content, it is necessary to first destroy the plant cell structure, and then hydrolyze the oxygen-ester bonds between the suberin monomers to release the suberin monomer components. This invention first uses ultrasonic pretreatment to destroy plant cells, and then uses lipase hydrolysis combined with ultrasonic assistance and acid hydrolysis (sulfuric acid / methanol) to fully and efficiently hydrolyze the suberin into monomers. The reagents used are relatively safe and non-toxic, and the operation is relatively simple, solving the technical problem of difficult determination of suberin monomer content in the study of suberin metabolism after yam tuber injury healing.

A surface treatment composition for increasing permeability in a hydrocarbon shale formation during stimulation containing a solvent and an effective amount of at least one of a quaternary monosaccharide having from about 1 to 3 moles of quaternary functional groups of and a quaternary saccharide having a number of the monosaccharide molecules ranging from about 2 to 20 bonded together by glycosidic linkage with from 1 to 3 moles of quaternary functional groups per molecule of the monosaccharide in the saccharide. A method of using the surface treatment for increasing permeability in a hydrocarbon shale formation during stimulation.

The invention provides a colonic microbiota responsive polymer characterized in that it is prepared by the polymerization of a response monomer and a linking monomer, wherein the response monomer is a cellobiose with a β-(1,4)-glucoside bond or / and a lactose with a β-galactoside bond; the linking monomer is a monomer that reacts with the hydroxyl group of the response monomer to form an ester or ether directly or by a crosslinking agent. The invention also provides a method for preparation of a colonic microbiota responsive polymer, a degradable film based on the polymer and a preparation method thereof. By means of the characteristics that β-(1,4)-glucoside bond provided by cellobiose or / and β-galactoside bond provided by lactose monomer unit in the polymer can specifically respond to the high abundance of microbiotas (β-glucosidase or / and β-galactosidase) in the colon to specifically degrade in the colon, a colon-targeted delivery system for diagnosis and treatment of colon diseases is constructed.

The invention discloses an exosome slow-release oral soluble film based on enzyme triggering and a preparation method thereof. The oral soluble film comprises a film forming substrate, a plasticizer, a disintegrating agent, mucous membrane adhesion type enzyme-sensitive nanogel and a stable protection component. Wherein the mucous membrane adhesion type enzyme-sensitive nanogel has a three-layer functional structure: an enzyme-sensitive cross-linking core is formed by maleimide starch through gradient cross-linking, and alpha-1, 4 glucosidic bonds on a starch chain can be hydrolyzed by saliva alpha-amylase; the phospholipid-exosome composite layer is located on the surface of the core, and efficient embedding of the exosome is achieved through a pre-fusion-cold locking process; the mucous membrane adhesion layer is made of thiolated mucous membrane adhesion materials and can form disulfide bonds with oral mucosa. Self-regulation slow release insensitive to saliva alpha-amylase concentration fluctuation is realized through a gradient cross-linked structure, the problems of exosome activity loss, uncontrollable release and weak adhesion are effectively solved, and the exosome is suitable for treatment of oral mucosa diseases.