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Application of chitosanoligosaccharide and derivatives thereof in plant cold resisting

A technology of chitosan oligosaccharides and derivatives, which is applied in the field of low temperature and freezing damage adversity resistance of plants, achieves the effect of less usage, solving food safety problems, and is suitable for large-scale promotion

Inactive Publication Date: 2012-11-14
DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are no literature and patent reports on using chitosan and its derivatives as plant antifreeze and cold resistance agents

Method used

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  • Application of chitosanoligosaccharide and derivatives thereof in plant cold resisting
  • Application of chitosanoligosaccharide and derivatives thereof in plant cold resisting
  • Application of chitosanoligosaccharide and derivatives thereof in plant cold resisting

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Embodiment 1 Commonly used this plant antifreeze anti-cold agent water preparation

[0035]The plant antifreeze and anti-cold agent is made into water agent, emulsifiable concentrate, soluble liquid, water emulsion, microemulsion, suspending agent by adding solvent and auxiliary agent; it is made into powder, soluble powder, wettable powder, granule by adding known carrier and auxiliary agent Agents, water dispersible granules, microcapsules, tablets. This embodiment has introduced a kind of commonly used water preparation proportioning, but the content of the present invention is not limited thereto: 75mg degree of polymerization 2-20, the chitosan oligosaccharide that degree of deacetylation is greater than 90% is dissolved in 1L water, makes 75ppm of this plant antifreeze anti-cold agent aqua. Wherein chitosan oligosaccharide content can be detected by following method:

[0036] High performance liquid chromatography-electrospray detector and Shodex NH2p-50 4E amin...

Embodiment 2

[0037] Embodiment 2 Use this plant antifreeze and anti-cold agent to improve the activity of tobacco resistance-related enzymes

[0038] Use the water agent in Example 1 to carry out leaf surface spraying treatment to tobacco, use water agent 60kg per mu, after seven days, sampling utilizes spectrophotometry to detect stress-resistant enzyme activity (detection method is with reference to: Chen Yafei etc., Functions of oligochitosan induced protein kinase in tobacco mosaic virus resistance and pathogenesis related proteins in tobacco.Plant Physiology and Biochemistry 47(2009)724-731; Yin Heng chitosan-oligosaccharide-induced antibacterial sclerotinia in rapeseed and its molecular mechanism Chinese Academy of Sciences Graduate School Doctoral Dissertation 2009), found The treatment group can greatly induce the activities of stress-resistant enzymes such as SOD, CAT, and POD to increase. The blank control was sprayed with the same amount of water. The specific results are shown...

Embodiment 3

[0041] Embodiment 3 plant antifreeze antifreeze agent is to the improvement of antifreeze of rapeseed

[0042] Taking 50% carbendazim wettable powder (Sichuan Guoguang Agrochemical Co., Ltd.) as a common plant antifreeze agent as a contrast, the active component of the plant antifreeze agent used is a shell with a molecular weight of 300-1600Da and a deacetylation degree of 95%. oligosaccharides. A total of four treatments were designed in the experiment: treatment 1, rapeseed bolting period + full flowering stage with 15g chitosan oligosaccharide mixed with 30kg of water and sprayed once; treatment 2, rapeseed bolting period + full flowering stage mu with 50% carbendazim 50g 30kg of water was sprayed once each; treatment 3, oligochitosan 10g per mu plus 50% carbendazim WP 50g, mixed with 30kg of water was sprayed once; treatment 4 was not sprayed (comparison). Each treatment was repeated three times, randomly arranged, (plot length 13.52m, width 7.4m) row hole spacing 0.74mx...

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Abstract

The invention discloses a wide-spectrum plant cold-resisting agent for plants such as food crops, economic crops, vegetables, fruit trees, economic trees, flowers, and lawns. The active component of the agent is chitosanoligosaccharide or a derivative thereof. The chitosanoligosaccharide is oligosaccharide of glucosamine linked through beta-1,4-glucosidic bond, and has a molecular weight of 300-10000Da, and a deacetylation degree of 50-100%. The chitosanoligosaccharide derivatives are chitosanoligosaccharide sulfuric acid derivative, chitosanoligosaccharide hydrochloric acid derivative, chitosanoligosaccharide phosphoric acid derivative, chitosanoligosaccharide sulfonamide derivative, chitosanoligosaccharide acyl isothiocyanate derivative, chitosanoligosaccharide phosphoric derivative, chitosanoligosaccharide guanidine derivative, chitosanoligosaccharide nicotinicacyl isothiocyanate derivative, chitosanoligosaccharide-cerium (IV) complex, and the like.

Description

technical field [0001] The invention relates to the adversity resistance of plants against low temperature and freezing damage, in particular to a plant antifreeze and anti-cold agent with chitosan oligosaccharide or chitosan oligosaccharide derivative as the main active substance and its application. Background technique [0002] Adversity stresses such as low temperature and freezing damage are extremely harmful to the growth of crops. The losses caused by plant adversity in my country are tens of billions of yuan each year. At present, these adversity stresses are usually dealt with by improving field management techniques or using chemical agricultural preparations. However, the excessive use of chemical agricultural preparations has gradually exposed problems such as low utilization rate, environmental pollution, and food safety, which has caused great negative impacts on agricultural production and the ecological environment, and has attracted widespread attention world...

Claims

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Application Information

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IPC IPC(8): A01N43/16A01P21/00A01G13/00
Inventor 赵小明杜昱光白雪芳尹恒刘启顺李曙光王文霞
Owner DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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