The invention discloses a method for ratio type colorimetric detection of
perchlorate. The method comprises the following steps: performing specific
surface modification on gold nanoparticles, constructing a
methylene blue functionalized gold
nanoparticle (MB (at) AuNPs) colorimetric probe, inducing a
particle aggregation effect by utilizing specific interaction between
perchlorate and MB (at) AuNPs, triggering
red shift of a localized surface
plasma resonance absorption peak of the probe, and detecting the concentration of the MB (at) AuNPs. And a 523 nm (dispersed state characteristic peak) and 660 nm (aggregation state characteristic peak) dual-
wavelength absorption
signal is formed. Ratio-type quantitative analysis is realized by calculating the ratio (A660 / A523) of the
absorbance of the two wavelengths, and the defect that single-
signal detection is easily influenced by environmental fluctuation and
system condition change is effectively avoided. Based on the
response characteristics, a dual quantitative relation between the ratio of the concentration of the
perchlorate to the
absorbance and the ratio of the
color intensity of an image collected by the
smart phone is further established, and an
ultraviolet-visible
spectrophotometry-
smart phone image analysis dual-mode detection
system is constructed. Wherein the
detection limit of the spectrum detection mode is 2.77 [mu] M, and the
detection limit of the
smart phone auxiliary detection mode is 5.63 [mu] M. The method is simple and convenient to operate, does not need a large-scale analytical instrument or complex sample pretreatment, has the advantages of good sensitivity, stability and
visual discrimination, can quickly realize on-site screening and accurate quantification of perchlorate in water and food samples, and is high in practicability and wide in popularization prospect.