The invention discloses a cisatracurium besilate content testing method which comprises the following steps: respectively weighing a test substance and a reference substance in a measuring flask, adding
hydrochloric acid for dissolving and metering a volume to a scale, shaking up to prepare a corresponding solution, finally, measuring the solution according to an
ultraviolet-visible
spectrophotometry, measuring
absorbance at a place of a maximum adsorption
wavelength of 280nm, and calculating the content of cisatracurium besilate according to an
anhydrous substance. According to the cisatracurium besilate content testing method, the solution is simply and conveniently prepared, no
organic solvent is used, and the
pollution to the environment and the harm to a
human body are small. The cisatracurium besilate content testing method is higher in testing speed, and the time of testing each batch of products in average only needs 30 min. A place of a maximum adsorption
wavelength of 280 nm is selected, so that the testing accuracy is ensured. The cost of a
reagent used in a process of checking each batch of samples does not exceed 20 yuan; the testing cost of each batch of samples by adopting a high-performance liquid
chromatography method is not less than 200 yuan, so that the cost of enterprises is greatly lowered.