152 results about "Spectrophotometry" patented technology

The invention relates to the field of betacyanin content detection, and discloses a method for rapidly detecting betacyanin content based on spectrophotometry, which comprises the following steps: experimental operation: preparing samples into standard solutions with different concentrations, carrying out full-spectrum scanning on the standard solutions by using a spectrophotometer, and determining the content of betacyanin by using a spectrophotometer; recording absorbance values under different wavelengths to form a spectral data matrix; preprocessing: preprocessing the spectral data matrix to obtain basic data; first analysis: analyzing the basic data by adopting a multivariate correction technology so as to obtain first data; and second analysis: analyzing the basic data by adopting a spectral feature extraction and mode recognition technology. A complete detection process is formed from data acquisition, preprocessing and analysis of different technologies to final fusion and integration, each step provides better data or more accurate analysis results for subsequent steps, and rapid and accurate detection of the betacyanin content is jointly realized.

A self-calibrating hybrid spectrophotometer comprises a receptacle for receiving the cuvette, first and second optodes that are configured to permit light to enter the cuvette, third and fourth optodes that are configured to permit light to leave the cuvette, and a controller that is configured to control delivery of light to one of the first and second optodes. Light entering one of the first and second optodes is received at the third and fourth optodes after having interacted with the turbid sample along different paths having different lengths. Measurements made at the third and fourth optodes in response to having illuminated both the first and second source optodes provide two slopes of optical transmittance as a function of path length. The spectrophotometer uses these slopes to identify a slope that is indicative of the absorption coefficient and the reduced scattering coefficient of the sample.

The invention discloses a cisatracurium besilate content testing method which comprises the following steps: respectively weighing a test substance and a reference substance in a measuring flask, adding hydrochloric acid for dissolving and metering a volume to a scale, shaking up to prepare a corresponding solution, finally, measuring the solution according to an ultraviolet-visible spectrophotometry, measuring absorbance at a place of a maximum adsorption wavelength of 280nm, and calculating the content of cisatracurium besilate according to an anhydrous substance. According to the cisatracurium besilate content testing method, the solution is simply and conveniently prepared, no organic solvent is used, and the pollution to the environment and the harm to a human body are small. The cisatracurium besilate content testing method is higher in testing speed, and the time of testing each batch of products in average only needs 30 min. A place of a maximum adsorption wavelength of 280 nm is selected, so that the testing accuracy is ensured. The cost of a reagent used in a process of checking each batch of samples does not exceed 20 yuan; the testing cost of each batch of samples by adopting a high-performance liquid chromatography method is not less than 200 yuan, so that the cost of enterprises is greatly lowered.

The invention provides a water quality detection method based on combination of machine learning and spectrophotometry, and the method comprises the steps: measuring the absorbance of a water sample under a plurality of preset wavelengths through a spectrophotometer to form a multi-dimensional absorbance data set, mapping the multi-dimensional absorbance data set to a high-dimensional space through a kernel function technology to obtain a data set with enhanced non-linear features, and carrying out the detection of the non-linear features of the multi-dimensional absorbance data set. Extracting a known water sample and the pollutant concentration corresponding to the known water sample from historical water quality data, generating a training sample set, performing pollutant concentration prediction on the data set with the enhanced nonlinear characteristics by applying a machine learning model, and dynamically adjusting the training sample set by adopting an active learning strategy to obtain a pollutant concentration prediction value, and performing safety grade evaluation on the water sample and outputting a water quality report by using the predicted value of the pollutant concentration in combination with a water quality safety standard, the technical scheme provided by the invention improves the efficiency and accuracy of water quality detection, provides powerful technical support for environmental protection and public health guarantee, and has a wide application prospect. The whole process realizes integrated operation from data acquisition to result interpretation, and ensures scientificity and reliability of water quality management decision.

To provide a spectrometry device that makes it possible to improve detection sensitivity to changes in physical properties such as expansion of a sample provided with energy by infrared radiation or the like, while also achieving high resolution and accurate autofocus.SOLUTION: A spectrometry device includes: an electromagnetic wave source for generating an electromagnetic wave to be emitted to a predetermined region of a sample; an objective lens for collecting an electromagnetic wave to the predetermined region; two shared focal point detectors for detecting an electromagnetic wave reflected by the sample; and a focus position determination mechanism for performing differential calculation or lock-in detection calculation on a signal obtained by dividing the difference signal and the sum signal of the outputs of each of the two shared focal point detectors and determining, as in-focus position, a position at which the peak value is obtained.SELECTED DRAWING: Figure 1

The invention relates to the technical field of raw malt, and particularly discloses a raw malt extraction method which comprises the following steps: preparing a phosphate buffer solution; preparing a boric acid buffer solution; preparing a derivatization solution by using the prepared boric acid buffer solution; the method comprises the following steps: breaking walls of dried raw malt and crushing into powder by using a multifunctional crusher to obtain raw malt powder, dissolving the raw malt powder in distilled water, and carrying out ultrasonic extraction to obtain a raw malt stock solution; after a trichloroacetic acid solution is added into the raw malt stock solution, the raw malt stock solution is subjected to centrifugal treatment, and supernate is obtained; adding a sodium hydroxide solution into the supernate to enable the pH value of the first mixed solution to be neutral, and adding a phosphate buffer solution into the first mixed solution to obtain a second mixed solution; carrying out sampling derivatization on the second mixed solution and the derivatization solution, and carrying out ultraviolet determination within 2min. By adopting the derivatization-ultraviolet spectrophotometry, the analysis time can be remarkably shortened, the operation complexity and cost can be reduced, and the method is more suitable for daily use in laboratories.

The invention provides an extraction and detection method of a day lily active matter and application of the day lily active matter in anti-neuritis. Hemerocallis fulva contains various bioactive substances including flavone, polysaccharide and saponin, and the substances have potential application value in the fields of medicines and health care products. The invention provides a method for extracting the active components step by step, and rapid identification and detection are carried out by utilizing technologies such as fluorescence and ultraviolet-visible spectrophotometry. In addition, the invention also develops a detection method for evaluating the anti-neuritis effect of the active substances. Lipopolysaccharide (LPS) is used for stimulation to establish an inflammation model, day lily active substances are added, and the active substances are found to be capable of remarkably inhibiting generation and release of inflammatory factors and promoting growth and differentiation of neurons. Scientific basis is provided for developing new neuritis treatment methods, related drugs and the like.

The invention discloses an organic-inorganic hybrid zero-dimensional halide perovskite single crystal material and a preparation method thereof. The chemical formula of the organic-inorganic hybrid zero-dimensional halide perovskite single crystal material is PMA4InCl7.H2O, and the chemical formula of the antimony-doped single crystal material is PMA4InCl7.H2O: Sb, the corresponding single crystal material is obtained through the steps of raw material adding, cooling crystallization, suction filtration, drying and the like. The optical band gap of the PMA4InCl7. H2O is determined to be 4-5 eV through an ultraviolet-visible spectrophotometer, and when the PMA4InCl7. H2O is irradiated by a 365 nm portable ultraviolet lamp, the PMA4InCl7. H2O shows light blue; the optical band gap of the PMA4InCl7. H2O: Sb is determined to be 3-4 eV through an ultraviolet-visible spectrophotometer, and when the PMA4InCl7. H2O: Sb is irradiated by a 365 nm portable ultraviolet lamp, the PMA4InCl7. H2O: Sb shows bright yellow. The maximum quantum yield measured by an absolute fluorescence quantum yield spectrometer can reach 72%.

The utility model provides a device for rapidly measuring the content of sulfate radicals, and relates to the technical field of water quality detection of water for polycrystalline silicon production. An inlet of the metering pump is connected with a sampling pipeline, and an outlet is connected with a conveying pipeline; the sampling pipeline is connected with the sample container; the conveying pipeline is connected with an inlet of the reaction container; the metering pump is provided with a detection mechanism which can detect the amount of samples conveyed by the metering pump; a detection reagent can be introduced into the reaction container, and the detection reagent is used for reacting with a sample to generate a colored product; the photoelectric detector is arranged close to the reaction container and is used for detecting the absorbance of the colored product; and the input end of the data processor is electrically connected with the output end of the photoelectric detector and the output end of the detection mechanism. Compared with an ultraviolet spectrophotometry, the method for determining the sulfate radical content of the water for polycrystalline silicon production based on the spectrophotometry has the advantages of higher detection precision and more convenient operation.

The invention belongs to the technical field of separation and detection, and particularly relates to a method for determining iodide in domestic drinking water. Measuring the iodide in the domestic drinking water by adopting a high performance liquid chromatography-ultraviolet detection method; taking a domestic drinking water sample solution for detection, and quantifying by an external standard method; according to the detection method, a Metrosep A Supp5-250 chromatographic column (250 mm * 4.0 mm, 5 [mu] m) is adopted for separation, 4.5 mmol / L Na2CO3, 1.0 mmol / L NaHCO3 and 35% acetonitrile are adopted as a mobile phase, a DAD detector is adopted, the wavelength is 226 nm, the flow rate is 0.60 mL / min, and the column temperature is 50 DEG C. The invention also discloses a method for detecting the content of the sodium hydroxide in the sample. According to methodology verification, the method can be used for measuring iodide in domestic drinking water, the detection limit is 2.0 mu g / L and is superior to that of a spectrophotometric method and a volumetric method in the national standard, no complex pretreatment process exists, specificity is high, toxic and harmful chemical reagents are not used, online automatic sample injection can be achieved, and the method has obvious advantages for large-batch samples; compared with inductively coupled plasma mass spectrometry, used instruments are relatively cheap, and basic popularization and application are facilitated.

The invention provides a method for rapidly detecting the ABTS < + > free radical scavenging rate of a traditional Chinese medicine extract based on Fourier infrared spectroscopy, which comprises the following steps: (1) collecting the Fourier infrared spectroscopy of a dandelion ethanol extract, and measuring the true value of the ABTS < + > free radical scavenging rate corresponding to the Fourier infrared spectroscopy by using spectrophotometry; (2) preprocessing the collected Fourier infrared spectrum to obtain a preprocessed spectrum; (3) carrying out characteristic wavelength variable extraction on the preprocessed spectrum by adopting a VIP algorithm, and combining spectrum segments corresponding to extracted characteristic wavelengths into a characteristic spectrum; (4) performing PCA analysis and step length segmentation processing on the characteristic spectrum to obtain an angle characteristic vector alpha y, and forming an angle characteristic matrix alpha by the angle characteristic vectors of all the traditional Chinese medicine extracts; and (5) constructing a regression model by using the angle feature vector matrix alpha. Compared with a traditional spectrophotometric detection method, the method has the advantage that the accuracy and the stability of a detection result can be better improved.

This invention provides a gas-phase molecular absorption spectrometry-naphthylethylenediamine hydrochloride spectrophotometric method for determining total nitrogen in waste gas from stationary pollution sources. The method comprises the following steps: S1. Reacting the waste gas with an oxidant; S2. Using an absorbent, further reacting the waste gas after S1; S3. Collecting the oxidant after S1, pretreating it to reduce nitrogen-containing compounds to nitric oxide, and detecting the total nitrogen content I; S4. Collecting the absorbent after S2 to obtain the total nitrogen content II; S5. Summing the total nitrogen content I and total nitrogen content II to obtain the total nitrogen content in the current waste gas. This method enables accurate determination of total nitrogen in waste gas from stationary pollution sources.

This invention provides a method and kit for detecting arsenic content using a thiol-modified MIL(53)-Fe metal-organic framework. Utilizing the enzyme-mimicking properties of the iron-based metal-organic framework, combined with the strong coordination effect of As(III) with thiol groups and the binding interaction between As(V) and iron, quantitative analysis of different forms of arsenic in water is achieved by visible light spectrophotometry. The linear range for As(III) detection in water is 0.2–1.6 ppb, with a detection limit of 0.172 ppb; the linear range for As(V) detection is 0.1–1.0 ppb, with a detection limit of 0.095 ppb. Furthermore, common coexisting elements in water do not significantly interfere with the quantitative detection of arsenic. Compared to existing arsenic speciation detection technologies, this invention offers advantages such as ease of operation, high sensitivity, and low cost, fully meeting the needs of relevant departments for online water quality monitoring and control.

The invention discloses preparation and visual detection application of an Ag / {001} Bi2Fe4O9 nanosheet, relates to the technical field of visual ultra-sensitive detection of 2, 4-dichlorophenol, and aims to realize an in-situ and ultra-sensitive visual detection method for the 2, 4-dichlorophenol. The method comprises the following steps: uniformly mixing a xanthene dye fluorescein aqueous solution, an Ag / {001} Bi2Fe4O9 nanosheet suspension and a 2, 4-dichlorophenol aqueous solution to obtain a solution to be detected; the method comprises the following steps: putting a solution to be detected into a Pomfiley multi-channel photocatalytic reactor, carrying out dark adsorption by stirring without illumination, then starting visible light, carrying out sufficient illumination, filtering by adopting a 22-micron water-phase filtering membrane, and finally testing by adopting an ultraviolet and visible spectrophotometer, thereby completing photosensitization detection of 2, 4-dichlorophenol. According to the invention, the Ag / {001} Bi2Fe4O9 nanosheet can be prepared and applied to visual detection.

The invention discloses a method for detecting the dissolution rate of ilaprazole. The invention provides a method for detecting the dissolution rate of ilaprazole. The dissolution rate of ilaprazole is detected by adopting an ultraviolet-visible spectrophotometric method. The detection method disclosed by the invention has the advantages of simplicity and convenience in operation, good repeatability, more stable dissolution rate data, better dissolution amount, convenience and rapidness, easiness in automation, more accurate and stable detection result and more suitability for industrial production.

The application discloses an improved iodine oxidation-dimethylglyoxime spectrophotometry for detecting the content of nickel in steel, which comprises the following steps: obtaining an acidolysis solution by acidolysis of the steel to be detected; then diluting the solution to obtain a mother liquor to be detected; mixing the mother liquor to be detected with an ammonium citrate solution, water, an iodine solution, ammonia water and a dimethylglyoxime solution to obtain a chromogenic solution to be detected; and placing the chromogenic solution to be detected below a critical temperature to perform spectrophotometry to obtain the content of nickel in the steel to be detected. The application controls the adding amount of the ammonium citrate and the dimethylglyoxime in the chromogenic solution to be detected and the chromogenic temperature, so that the stable time of the absorbance of the chromogenic solution to be detected is greater than or equal to 60 minutes, the detection of the content of copper in the steel to be detected is less than or equal to 0.2%, and the probability of the result of the detection of the content of nickel in the steel to be detected (0.10%-50%) exceeding the allowable error of a navigation mark is less than or equal to 5%.

A system provides for an ability to automatically identify one or more chemical components of a sample, especially a biological fluid sample, based on analysis of spectral data by at least one artificial intelligence module. The artificial intelligence module is able to be trained on a plurality of spectral data samples having known concentrations of individual chemicals and elements. The system is preferably used for spectral techniques such as UV / vis and near-infrared (NIR) spectrophotometry, but is also able to be used for other forms of spectroscopic techniques, including mass spectrometry, infrared (IR) spectrometry, X-ray spectroscopy, Raman spectrometry, or nuclear magnetic resonance (NMR) spectrometry.

The invention provides a method for enhancing heavy metal ion concentration analysis and detection sensitivity and application. The method comprises the following steps: S1, carrying out gradient dilution on a heavy metal solution and a zinc ion solution to obtain a dilute solution with a series of concentration gradients; s2, measuring the absorption values of the dilute solutions with the series of concentration gradients by adopting a spectrophotometric method, and establishing a standard curve; according to the method, the zinc ions are introduced as interference variables when the standard curve for measuring the heavy metal ions is constructed, the slope of the standard curve can be remarkably increased by adding the zinc ions, the analysis and detection sensitivity of the spectrophotometric method can be effectively improved, and a new sensitivity optimization strategy is provided for heavy metal ion detection.

The invention relates to the technical field of concentration detection of phosphate, in particular to a method for detecting the concentration of phosphate ions in a nickel-free hole sealing tank solution. The detection method comprises the following steps: adding a nickel-free hole sealing tank solution into a quantitative bottle for quantification, adding an ascorbic acid solution and a molybdate solution into a volumetric flask, carrying out a standing reaction at 28-32 DEG C for 15-25 min, determining the light absorption value of the reaction solution at 700 nm by using a spectrophotometer, obtaining the phosphorus content M according to a phosphorus standard curve model, and calculating the phosphate ion concentration P according to a formula. Compared with an existing method for detecting the concentration of orthophosphoric acid through IC equipment, the concentration data measured through the spectrophotometric method is more stable and smaller in difference, the accuracy of concentration analysis of the phosphate ions in the nickel-free hole sealing groove can be achieved, and the situation that materials are scrapped due to the fact that the concentration of the phosphate ions is abnormal in the production process is effectively avoided.

The application discloses a double-optical-path spectrometer, a color measurement device and a calibration method, and relates to the field of color measurement devices. The color measurement device obtains reflected light of a measured sample and reflected light of a test light source, and inputs the reflected light into a first incident slit and a second incident slit of a spectrometer through a Y-shaped light path unit; the reflected light is incident on a grating through a lens; diffracted light spots returned by the grating are incident on a photosensitive surface of a sensor on the side of the incident slit through the lens; the photosensitive surface is provided with an aperture stop with a dislocation opening; and the photosensitive surface at each dislocation opening only receives light signals of one light path, so that the short-term repeatability of spectrophotometry is improved. When wavelength calibration is performed, light signals of a calibration light source are obtained through the Y-shaped light path unit; pixel position information of the collected light signals is obtained according to a sensor of the spectrometer; a fourth-order polynomial is used to fit wavelength data and the pixel position information; the correspondence between the pixels and the wavelengths is adjusted; and the wavelengths are calibrated, so that the short-term repeatability of spectrophotometry is improved.

The utility model discloses a water quality analyzer which comprises a shifting turntable, a liquid inlet module, a light source, a detector, a color sensor and a control module, a liquid adding position, a detection position and a first detection container are arranged on the shifting turntable, and the detection position comprises a light splitting detection position and a titration detection position; the liquid inlet module comprises a liquid adding head, and the liquid adding head comprises a first liquid adding head; a first liquid adding head is arranged above each of the liquid adding position and the titration detection position; the first detection container can move to a liquid adding position and a detection position along with the shifting turntable; the light source and the detector are oppositely arranged on the two sides of the light splitting detection position and correspond to the first detection container in position; the color sensor is used for monitoring solution color; the control module is connected with the liquid inlet module, the displacement turntable, the light source, the detector and the color sensor. The device is suitable for on-line detection of various water quality detection items which can be detected by utilizing a spectrometric indexing method, a titration method and an electrode method, the titration end point is accurately judged during titration analysis, and the titration analysis result is relatively accurate.

The invention relates to a method for detecting the content of polysaccharide in a ginseng-radix puerariae kidney-tonifying preparation, which is characterized in that after a test solution is subjected to chromatography treatment, interference components of radix pseudostellariae polysaccharide in the ginseng-radix puerariae kidney-tonifying preparation can be effectively separated, and meanwhile, a polysaccharide detection and analysis method of the ginseng-radix puerariae kidney-tonifying preparation is established by combining ultraviolet-visible spectrophotometry. Besides, methodology verifies that the method is good in specificity, accuracy, precision, repeatability, stability and durability, an effective evaluation method can be provided for quality control of the ginseng-radix puerariae kidney-tonifying preparation, and the method can be suitable for detection of multiple batches of ginseng-radix puerariae kidney-tonifying preparations so as to inspect the specificity difference between different batches of preparations.

The present application discloses an online monitoring system for total thallium in water quality. The online monitoring system for total thallium in water quality includes: a water quality sampling module for periodically sampling water quality samples; a total thallium detection module for detecting the total thallium content in the water quality samples according to a preset spectrophotometry based on o-hydroxybenzenediazoaminoazobenzene; and a result output module for generating and outputting the monitoring result of total thallium in water quality according to the total thallium content. The present application solves the technical problem of poor timeliness in monitoring total thallium in water quality in the prior art.

The invention discloses a method for determining the content of semicarbazide in a plastic product, and belongs to the technical field of analytical testing, the method is a spectrophotometric method, and the method specifically comprises the following steps: firstly, carrying out derivatization treatment by adopting benzoyl chloride, and then carrying out absorbance detection at 280nm; compared with a liquid chromatography-mass spectrometry method, the method is faster and more efficient in detection and suitable for rapid detection occasions and checkout rooms with low investment cost requirements, the lowest detection capacity of the method is 0.1 mg / L, most quantitative requirements are met, the pretreatment process is simple, the derivation time is short, the operation process is easy to master, and the method is suitable for industrial production. And the result can be obtained only in about 10 minutes in the conventional detection, so that the timeliness and the safety are very high.

The invention provides a laser absorption and LIF imaging fused water quality COD detection method and system, and belongs to the technical field of water quality monitoring, the method comprises the following steps: placing a to-be-detected water sample in a sample pool, and vertically irradiating the water sample with laser; performing high-concentration COD detection by adopting an ultraviolet absorption spectrophotometry based on a Lambert-Beer law in combination with a calibration curve; a laser induced fluorescence (LIF) imaging technology is adopted, the RGB gray value of the ROI of a fluorescence image is extracted, and low-concentration COD detection is carried out in combination with a fluorescence intensity-COD model; automatically matching and detecting according to the concentration of the water sample, and outputting a final COD detection value; the system comprises a laser emission module, an image acquisition module, a sample detection module, a transmission light detection module and a data processing module. The advantages of two optical detection technologies are fused, rapid and accurate detection of COD of various water bodies is achieved, the system sensitivity is high, the structure is compact, and real-time monitoring of COD of the water bodies is achieved.

The application discloses a pesticide detection method for rice based on QuEChERS and ultraviolet-visible spectrophotometry, and takes three pesticides, i.e., bensulfuron-methyl, propanil and cypermethrin, as examples to carry out research on a rapid screening method for multiple pesticide residues in rice. The improved QuEChERS method is used to process rice to obtain a matrix liquid, matrix standard solution and solvent standard solution are prepared, the quantitative linear range of each pesticide and the matrix effect are obtained. In the quantitative concentration range, mixed solutions of the three pesticides in four combinations are prepared, the types of the pesticides in the rice are determined through support vector machine qualitative analysis, and the concentrations of the pesticides are predicted through partial least squares regression analysis, so that the pesticide residues in the rice can be effectively and rapidly detected.

The invention discloses a method for detecting potential electric leakage defects of an LED device, and relates to the technical field of semiconductor photoelectric device detection. Aiming at the technical pain point that recessive microdefects (such as a metal migration channel caused by a V-shaped pit and dislocation) cannot be identified by the existing single spectroscopic detection, the invention provides a detection logic of reference parameter acquisition, defect stress excitation, parameter acquisition after excitation and differential analysis and judgment. Specific stress is applied to primarily screened qualified products, ion migration at potential defects is excited in an oriented mode, and hidden electric leakage is dominated. Potential defective products are accurately rejected by calculating parameter drift distances (VFL, IR) before and after stress. The method is not only suitable for interactive screens, but also suitable for high-reliability scenes such as vehicle-mounted display and high-end illumination, and can effectively intercept hidden lamp beads causing'caterpillar 'failure or early light attenuation, thereby remarkably improving the product yield and the terminal reliability.

The embodiment of the application provides a kind of miniature infrared spectrometer and electronic equipment, for environmental monitoring, biological medical treatment, food safety etc..The spectrometer additionally increases narrow-band light source, for emitting second light beam, by micro stepping motor control plane grating, the first background spectrum and second background spectrum are generated in linear array detector by the second light beam spectrophotometry;In addition, the first light beam is emitted to the surface of the object to be measured by wide spectrum light source and occurs diffuse reflection, by micro stepping motor control plane grating, the first mixed spectrum and second mixed spectrum are generated in linear array detector by the mixed light beam spectrophotometry of first light beam and second light beam.Finally, the spectrum of the object to be measured is determined by the spectrum of narrow-band light source, first mixed spectrum and second mixed spectrum, and first background spectrum and second background spectrum by linear array detector.The technical scheme of the application can further compress the volume of spectrometer, reduce cost, and also can realize wide spectral range detection.

The invention discloses a molybdenum alloy total molybdenum content detection method based on a lead molybdate gravimetric method. The method comprises the following steps: dissolving a ferromolybdenum alloy sample through a nitric acid-potassium chlorate saturated solution and the like, carrying out lead molybdate precipitation through ammonia water, an acetic acid-ammonium acetate buffer solution, lead acetate and the like, carrying out high-temperature gradient sintering on filter residues through a mixed solvent of anhydrous sodium carbonate and zinc oxide, and determining residual molybdenum through a potassium thiocyanate spectrophotometric method after dissolving. And performing correction calculation. According to the method, a gravimetric method and a spectrophotometric method are combined for use, the filter residues are corrected, the precipitation process technology is optimized, the problems that a traditional method is tedious in operation and large in residual molybdenum error of the filter residues are solved, and high-precision, high-efficiency and low-cost molybdenum content detection is achieved.

The invention relates to a hand-held device (100) for spectrophotometry, comprising: a) a housing (110) having an opening for a light beam to enter the hand-held device from a surface; b) a device (120) for determining a reflection value of the light beam; and c) an interface (140) for a mobile device (200), the interface providing a signal indicative of the reflection values to the mobile device. Accordingly, a handheld device for spectrophotometry is provided in which a mobile device is used as a user interface.