173 results about "Spectrophotometry" patented technology

A lithium ion-conductive solid electrolyte including a freestanding inorganic vitreous sheet of sulfide-based lithium ion conducting glass is capable of high performance in a lithium metal battery by providing a high degree of lithium ion conductivity while being highly resistant to the initiation and / or propagation of lithium dendrites. Such an electrolyte is also itself manufacturable, and readily adaptable for battery cell and cell component manufacture, in a cost-effective, scalable manner. An automated machine based system, apparatus and methods assessing and inspecting the quality of such vitreous solid electrolyte sheets, electrode sub-assemblies and lithium electrode assemblies can be based on spectrophotometry and can be performed inline with fabricating the sheet or web (e.g., inline with drawing of the vitreous Li ion conducting glass) and / or with the manufacturing of associated electrode sub-assemblies and lithium electrode assemblies and battery cells.

The invention relates to the field of betacyanin content detection, and discloses a method for rapidly detecting betacyanin content based on spectrophotometry, which comprises the following steps: experimental operation: preparing samples into standard solutions with different concentrations, carrying out full-spectrum scanning on the standard solutions by using a spectrophotometer, and determining the content of betacyanin by using a spectrophotometer; recording absorbance values under different wavelengths to form a spectral data matrix; preprocessing: preprocessing the spectral data matrix to obtain basic data; first analysis: analyzing the basic data by adopting a multivariate correction technology so as to obtain first data; and second analysis: analyzing the basic data by adopting a spectral feature extraction and mode recognition technology. A complete detection process is formed from data acquisition, preprocessing and analysis of different technologies to final fusion and integration, each step provides better data or more accurate analysis results for subsequent steps, and rapid and accurate detection of the betacyanin content is jointly realized.

The invention provides a food pollution detection method based on multi-wavelength spectrophotometry, and the method comprises the steps: transmitting light in a series of wavelength ranges to a food sample through a tunable multi-wavelength light source system, generating spectral response, collecting the spectral response through a high-resolution spectrometer, and forming a multi-dimensional spectral image, a deep learning algorithm is used for analyzing, identifying and distinguishing subtle spectral differences between natural components and potential pollutants in a food sample, a preliminary identification result is obtained, a self-adaptive wavelength selection mechanism is implemented, wavelength output of a light source is dynamically adjusted, spectral response data is obtained, and the spectral response data is compared with a preset food safety standard database. According to the technical scheme provided by the invention, natural components and potential pollutants in a food sample are efficiently and accurately recognized, and the sensitivity, specificity and accuracy of pollutant detection are remarkably improved.

The invention discloses an ammonia nitrogen detection system and an application method thereof, and ammonia nitrogen detection test paper, the ammonia nitrogen detection system is prepared by the following steps: step SA1) preparing a dipping mixed solution, the dipping mixed solution comprises: a potassium sodium tartrate solution with a concentration of 3-60 g / L; a sodium salicylate solution with a concentration of 20-320 g / L; the concentration of the sodium nitroprusside solution is 0.1 to 0.8 g / L; and deionized water. On the basis of the principle of salicylic acid-pypocholoride spectrophotometry, the impregnated test paper, the reagent tube and the standard colorimetric card are combined, and the rapid ammonia nitrogen detection system special for medium-concentration sewage detection is successfully developed. The toxicity problem of mercury iodide in a Nessler reagent method and the toxicity problem of phenol in a phenol-pypocholoride spectrophotometric method are avoided, main reagents are non-toxic and harmless to a human body, interference of metal ions in a water sample can be effectively eliminated by adopting sodium potassium tartrate tetrahydrate as a chelating agent, and the detection stability and accuracy are improved.

A self-calibrating hybrid spectrophotometer comprises a receptacle for receiving the cuvette, first and second optodes that are configured to permit light to enter the cuvette, third and fourth optodes that are configured to permit light to leave the cuvette, and a controller that is configured to control delivery of light to one of the first and second optodes. Light entering one of the first and second optodes is received at the third and fourth optodes after having interacted with the turbid sample along different paths having different lengths. Measurements made at the third and fourth optodes in response to having illuminated both the first and second source optodes provide two slopes of optical transmittance as a function of path length. The spectrophotometer uses these slopes to identify a slope that is indicative of the absorption coefficient and the reduced scattering coefficient of the sample.

The invention discloses a system and a method for verifying and evaluating the optical performance of a partial discharge optical sensor based on integral spectrophotometry, and the optical sensor is used for capturing an optical signal generated in a partial discharge process, and converting the optical signal into an electric signal; secondly, the optical signals are processed through an integral spectrophotometry, and optical parameters such as luminous flux, spectral distribution and light intensity are calculated; and then, based on an algorithm in the data acquisition and processing unit, the response characteristic, the sensitivity and the measurement precision of the optical sensor are evaluated. According to the technical scheme, all-around performance verification and accurate evaluation of the partial discharge optical sensor can be achieved, the detection precision and reliability of the optical sensor in complex power equipment are improved, and the method has wide application prospects.

The invention discloses a cisatracurium besilate content testing method which comprises the following steps: respectively weighing a test substance and a reference substance in a measuring flask, adding hydrochloric acid for dissolving and metering a volume to a scale, shaking up to prepare a corresponding solution, finally, measuring the solution according to an ultraviolet-visible spectrophotometry, measuring absorbance at a place of a maximum adsorption wavelength of 280nm, and calculating the content of cisatracurium besilate according to an anhydrous substance. According to the cisatracurium besilate content testing method, the solution is simply and conveniently prepared, no organic solvent is used, and the pollution to the environment and the harm to a human body are small. The cisatracurium besilate content testing method is higher in testing speed, and the time of testing each batch of products in average only needs 30 min. A place of a maximum adsorption wavelength of 280 nm is selected, so that the testing accuracy is ensured. The cost of a reagent used in a process of checking each batch of samples does not exceed 20 yuan; the testing cost of each batch of samples by adopting a high-performance liquid chromatography method is not less than 200 yuan, so that the cost of enterprises is greatly lowered.

The invention provides a water quality detection method based on combination of machine learning and spectrophotometry, and the method comprises the steps: measuring the absorbance of a water sample under a plurality of preset wavelengths through a spectrophotometer to form a multi-dimensional absorbance data set, mapping the multi-dimensional absorbance data set to a high-dimensional space through a kernel function technology to obtain a data set with enhanced non-linear features, and carrying out the detection of the non-linear features of the multi-dimensional absorbance data set. Extracting a known water sample and the pollutant concentration corresponding to the known water sample from historical water quality data, generating a training sample set, performing pollutant concentration prediction on the data set with the enhanced nonlinear characteristics by applying a machine learning model, and dynamically adjusting the training sample set by adopting an active learning strategy to obtain a pollutant concentration prediction value, and performing safety grade evaluation on the water sample and outputting a water quality report by using the predicted value of the pollutant concentration in combination with a water quality safety standard, the technical scheme provided by the invention improves the efficiency and accuracy of water quality detection, provides powerful technical support for environmental protection and public health guarantee, and has a wide application prospect. The whole process realizes integrated operation from data acquisition to result interpretation, and ensures scientificity and reliability of water quality management decision.

To provide a spectrometry device that makes it possible to improve detection sensitivity to changes in physical properties such as expansion of a sample provided with energy by infrared radiation or the like, while also achieving high resolution and accurate autofocus.SOLUTION: A spectrometry device includes: an electromagnetic wave source for generating an electromagnetic wave to be emitted to a predetermined region of a sample; an objective lens for collecting an electromagnetic wave to the predetermined region; two shared focal point detectors for detecting an electromagnetic wave reflected by the sample; and a focus position determination mechanism for performing differential calculation or lock-in detection calculation on a signal obtained by dividing the difference signal and the sum signal of the outputs of each of the two shared focal point detectors and determining, as in-focus position, a position at which the peak value is obtained.SELECTED DRAWING: Figure 1

Systems, devices, and methods herein may include a system for monitoring bodily fluid from a patient. In some embodiments, the system may be configured to collect bodily fluid samples from a patient periodically over time to determine a baseline bodily fluid composition. In some embodiments, the system may use spectrophotometry to determine the baseline bodily fluid composition for the patient. The system may be configured to compare a bodily fluid sample to the baseline bodily fluid composition to detect detecting changes in one or more characteristics of the bodily fluid sample (e.g., composition, absorption spectrum, etc.) from the baseline. Changes in the bodily fluid sample characteristics may be used to predict and / or detect abnormalities or infection for individualized diagnosis.

The invention relates to the technical field of raw malt, and particularly discloses a raw malt extraction method which comprises the following steps: preparing a phosphate buffer solution; preparing a boric acid buffer solution; preparing a derivatization solution by using the prepared boric acid buffer solution; the method comprises the following steps: breaking walls of dried raw malt and crushing into powder by using a multifunctional crusher to obtain raw malt powder, dissolving the raw malt powder in distilled water, and carrying out ultrasonic extraction to obtain a raw malt stock solution; after a trichloroacetic acid solution is added into the raw malt stock solution, the raw malt stock solution is subjected to centrifugal treatment, and supernate is obtained; adding a sodium hydroxide solution into the supernate to enable the pH value of the first mixed solution to be neutral, and adding a phosphate buffer solution into the first mixed solution to obtain a second mixed solution; carrying out sampling derivatization on the second mixed solution and the derivatization solution, and carrying out ultraviolet determination within 2min. By adopting the derivatization-ultraviolet spectrophotometry, the analysis time can be remarkably shortened, the operation complexity and cost can be reduced, and the method is more suitable for daily use in laboratories.

The invention provides an extraction and detection method of a day lily active matter and application of the day lily active matter in anti-neuritis. Hemerocallis fulva contains various bioactive substances including flavone, polysaccharide and saponin, and the substances have potential application value in the fields of medicines and health care products. The invention provides a method for extracting the active components step by step, and rapid identification and detection are carried out by utilizing technologies such as fluorescence and ultraviolet-visible spectrophotometry. In addition, the invention also develops a detection method for evaluating the anti-neuritis effect of the active substances. Lipopolysaccharide (LPS) is used for stimulation to establish an inflammation model, day lily active substances are added, and the active substances are found to be capable of remarkably inhibiting generation and release of inflammatory factors and promoting growth and differentiation of neurons. Scientific basis is provided for developing new neuritis treatment methods, related drugs and the like.

The invention relates to a method for identifying soapberry from soapberry peel based on ultraviolet-visible spectrophotometry. The method is characterized by comprising the following steps: preparing a saponin solution, adding a 5% vanillin-glacial acetic acid solution and perchloric acid, uniformly shaking, heating in a constant-temperature water bath kettle at 70 DEG C for 15 minutes, cooling in an ice-water bath for 3 minutes, adding 5mL glacial acetic acid as a solvent, and measuring the characteristic absorption spectra of the vanillin and the glacial acetic acid by adopting an ultraviolet and visible spectrophotometer. And by utilizing the obvious difference of the number, the peak pattern and the position of the characteristic peaks in the absorption spectrum, the simple and rapid identification of the two is realized. The method has the advantages that the mass of required raw materials is small, the operation is simple, the process flow is short, and the experimental conditions are easy to realize. The technology is a rapid and efficient method for identifying and identifying the pericarp, pericarp powder and pericarp extract of the Sapindus maxima and the Sapindus mukorossi.

The invention discloses an organic-inorganic hybrid zero-dimensional halide perovskite single crystal material and a preparation method thereof. The chemical formula of the organic-inorganic hybrid zero-dimensional halide perovskite single crystal material is PMA4InCl7.H2O, and the chemical formula of the antimony-doped single crystal material is PMA4InCl7.H2O: Sb, the corresponding single crystal material is obtained through the steps of raw material adding, cooling crystallization, suction filtration, drying and the like. The optical band gap of the PMA4InCl7. H2O is determined to be 4-5 eV through an ultraviolet-visible spectrophotometer, and when the PMA4InCl7. H2O is irradiated by a 365 nm portable ultraviolet lamp, the PMA4InCl7. H2O shows light blue; the optical band gap of the PMA4InCl7. H2O: Sb is determined to be 3-4 eV through an ultraviolet-visible spectrophotometer, and when the PMA4InCl7. H2O: Sb is irradiated by a 365 nm portable ultraviolet lamp, the PMA4InCl7. H2O: Sb shows bright yellow. The maximum quantum yield measured by an absolute fluorescence quantum yield spectrometer can reach 72%.

The utility model provides a device for rapidly measuring the content of sulfate radicals, and relates to the technical field of water quality detection of water for polycrystalline silicon production. An inlet of the metering pump is connected with a sampling pipeline, and an outlet is connected with a conveying pipeline; the sampling pipeline is connected with the sample container; the conveying pipeline is connected with an inlet of the reaction container; the metering pump is provided with a detection mechanism which can detect the amount of samples conveyed by the metering pump; a detection reagent can be introduced into the reaction container, and the detection reagent is used for reacting with a sample to generate a colored product; the photoelectric detector is arranged close to the reaction container and is used for detecting the absorbance of the colored product; and the input end of the data processor is electrically connected with the output end of the photoelectric detector and the output end of the detection mechanism. Compared with an ultraviolet spectrophotometry, the method for determining the sulfate radical content of the water for polycrystalline silicon production based on the spectrophotometry has the advantages of higher detection precision and more convenient operation.

The invention belongs to the technical field of separation and detection, and particularly relates to a method for determining iodide in domestic drinking water. Measuring the iodide in the domestic drinking water by adopting a high performance liquid chromatography-ultraviolet detection method; taking a domestic drinking water sample solution for detection, and quantifying by an external standard method; according to the detection method, a Metrosep A Supp5-250 chromatographic column (250 mm * 4.0 mm, 5 [mu] m) is adopted for separation, 4.5 mmol / L Na2CO3, 1.0 mmol / L NaHCO3 and 35% acetonitrile are adopted as a mobile phase, a DAD detector is adopted, the wavelength is 226 nm, the flow rate is 0.60 mL / min, and the column temperature is 50 DEG C. The invention also discloses a method for detecting the content of the sodium hydroxide in the sample. According to methodology verification, the method can be used for measuring iodide in domestic drinking water, the detection limit is 2.0 mu g / L and is superior to that of a spectrophotometric method and a volumetric method in the national standard, no complex pretreatment process exists, specificity is high, toxic and harmful chemical reagents are not used, online automatic sample injection can be achieved, and the method has obvious advantages for large-batch samples; compared with inductively coupled plasma mass spectrometry, used instruments are relatively cheap, and basic popularization and application are facilitated.

The invention provides a method for rapidly detecting the ABTS < + > free radical scavenging rate of a traditional Chinese medicine extract based on Fourier infrared spectroscopy, which comprises the following steps: (1) collecting the Fourier infrared spectroscopy of a dandelion ethanol extract, and measuring the true value of the ABTS < + > free radical scavenging rate corresponding to the Fourier infrared spectroscopy by using spectrophotometry; (2) preprocessing the collected Fourier infrared spectrum to obtain a preprocessed spectrum; (3) carrying out characteristic wavelength variable extraction on the preprocessed spectrum by adopting a VIP algorithm, and combining spectrum segments corresponding to extracted characteristic wavelengths into a characteristic spectrum; (4) performing PCA analysis and step length segmentation processing on the characteristic spectrum to obtain an angle characteristic vector alpha y, and forming an angle characteristic matrix alpha by the angle characteristic vectors of all the traditional Chinese medicine extracts; and (5) constructing a regression model by using the angle feature vector matrix alpha. Compared with a traditional spectrophotometric detection method, the method has the advantage that the accuracy and the stability of a detection result can be better improved.

The invention discloses a method for measuring aluminum content by chrome azuro blue S spectrophotometry, comprising: digesting a sample, removing acid, and fixing the volume to obtain a digestion solution; removing the digestion solution, adding an appropriate amount of sodium hydroxide solution, standing for a period of time, and centrifuging to obtain a first supernatant and a first precipitate; taking the first precipitate, adding an appropriate amount of sodium hydroxide solution, and vortexing, transferring the vortexed solution to a microwave digestion tank, performing microwave treatment, and after the microwave treatment, centrifuging the solution again to obtain a second supernatant and a second precipitate; combining the first supernatant and the second supernatant, adding an appropriate amount of nitric acid, and performing heating and concentration treatment to obtain a solution to be tested. The invention can accurately measure the aluminum content by using the chrome azuro blue S spectrophotometry when a large amount of iron is contained in food.

The invention discloses an ICP-OES (Inductively Coupled Plasma Optical Emission Spectrometer) method for determining the content of cerium in aviation gear steel. The method comprises the following steps: setting working parameters of an inductively coupled plasma emission spectrometer; determining the blank solution and the cerium standard solutions under different concentrations by using an inductively coupled plasma emission spectrometer, and drawing a cerium element concentration standard curve; determining a to-be-determined sample solution by using the inductively coupled plasma emission spectrometer, and then determining the content of the cerium element in the to-be-determined solution by combining the cerium element concentration standard curve. According to the method, the content of the cerium element in the aviation gear steel is accurately measured, meanwhile, it is guaranteed that other elements are accurately measured, no element loss is generated, and compared with a steel mill test result, a sulfuric acid-phosphoric acid fuming method, a perchloric acid fuming method and a spectrophotometric method, the difference is small, precision is high, and accuracy and reliability are achieved.

This invention provides a gas-phase molecular absorption spectrometry-naphthylethylenediamine hydrochloride spectrophotometric method for determining total nitrogen in waste gas from stationary pollution sources. The method comprises the following steps: S1. Reacting the waste gas with an oxidant; S2. Using an absorbent, further reacting the waste gas after S1; S3. Collecting the oxidant after S1, pretreating it to reduce nitrogen-containing compounds to nitric oxide, and detecting the total nitrogen content I; S4. Collecting the absorbent after S2 to obtain the total nitrogen content II; S5. Summing the total nitrogen content I and total nitrogen content II to obtain the total nitrogen content in the current waste gas. This method enables accurate determination of total nitrogen in waste gas from stationary pollution sources.

This invention provides a method and kit for detecting arsenic content using a thiol-modified MIL(53)-Fe metal-organic framework. Utilizing the enzyme-mimicking properties of the iron-based metal-organic framework, combined with the strong coordination effect of As(III) with thiol groups and the binding interaction between As(V) and iron, quantitative analysis of different forms of arsenic in water is achieved by visible light spectrophotometry. The linear range for As(III) detection in water is 0.2–1.6 ppb, with a detection limit of 0.172 ppb; the linear range for As(V) detection is 0.1–1.0 ppb, with a detection limit of 0.095 ppb. Furthermore, common coexisting elements in water do not significantly interfere with the quantitative detection of arsenic. Compared to existing arsenic speciation detection technologies, this invention offers advantages such as ease of operation, high sensitivity, and low cost, fully meeting the needs of relevant departments for online water quality monitoring and control.

The invention discloses preparation and visual detection application of an Ag / {001} Bi2Fe4O9 nanosheet, relates to the technical field of visual ultra-sensitive detection of 2, 4-dichlorophenol, and aims to realize an in-situ and ultra-sensitive visual detection method for the 2, 4-dichlorophenol. The method comprises the following steps: uniformly mixing a xanthene dye fluorescein aqueous solution, an Ag / {001} Bi2Fe4O9 nanosheet suspension and a 2, 4-dichlorophenol aqueous solution to obtain a solution to be detected; the method comprises the following steps: putting a solution to be detected into a Pomfiley multi-channel photocatalytic reactor, carrying out dark adsorption by stirring without illumination, then starting visible light, carrying out sufficient illumination, filtering by adopting a 22-micron water-phase filtering membrane, and finally testing by adopting an ultraviolet and visible spectrophotometer, thereby completing photosensitization detection of 2, 4-dichlorophenol. According to the invention, the Ag / {001} Bi2Fe4O9 nanosheet can be prepared and applied to visual detection.

One form of the spectrophotometer of the present invention comprises: a spectrum measuring section (11, 12) for repeatedly measuring a spectrum of a predetermined wavelength range for a light to be measured, which is laser light; a peak counting section (21, 22) for detecting a peak from the spectrum and counting the number of detected peaks each time the spectrum is obtained by the spectrum measuring section; and a display processing section (24) for displaying the numerical value of the peak counting result obtained by the peak counting section on a screen of the display section in real time. According to the spectrophotometer of the form, the adjustment of a multi-mode laser oscillator can be performed efficiently and accurately.

The invention discloses equipment for detecting the content of ozone in a solution. The detection equipment comprises a sample chamber, a detection unit and a processor, the sample chamber comprises an accommodating cavity for accommodating test liquid, and the accommodating cavity is provided with a liquid inlet and a liquid outlet; the detection unit comprises an ultraviolet emitter and a detector, and the wavelength window of the ultraviolet emitter is set to be 253.7 nm + / -0.3 nm; the detector comprises an ultraviolet light sensor; the processor is used for receiving the initial concentration transmitted by the detection unit, a concentration correction module is arranged in the processor, the concentration correction module corrects the initial concentration by using correction parameters to obtain the ozone content concentration, and the correction parameters are obtained by combining an ultraviolet spectrophotometric method and an iodometric titration method. In this way, the accuracy of testing the ozone content in the solution can be improved.

The invention discloses a method for detecting the dissolution rate of ilaprazole. The invention provides a method for detecting the dissolution rate of ilaprazole. The dissolution rate of ilaprazole is detected by adopting an ultraviolet-visible spectrophotometric method. The detection method disclosed by the invention has the advantages of simplicity and convenience in operation, good repeatability, more stable dissolution rate data, better dissolution amount, convenience and rapidness, easiness in automation, more accurate and stable detection result and more suitability for industrial production.

The application discloses an improved iodine oxidation-dimethylglyoxime spectrophotometry for detecting the content of nickel in steel, which comprises the following steps: obtaining an acidolysis solution by acidolysis of the steel to be detected; then diluting the solution to obtain a mother liquor to be detected; mixing the mother liquor to be detected with an ammonium citrate solution, water, an iodine solution, ammonia water and a dimethylglyoxime solution to obtain a chromogenic solution to be detected; and placing the chromogenic solution to be detected below a critical temperature to perform spectrophotometry to obtain the content of nickel in the steel to be detected. The application controls the adding amount of the ammonium citrate and the dimethylglyoxime in the chromogenic solution to be detected and the chromogenic temperature, so that the stable time of the absorbance of the chromogenic solution to be detected is greater than or equal to 60 minutes, the detection of the content of copper in the steel to be detected is less than or equal to 0.2%, and the probability of the result of the detection of the content of nickel in the steel to be detected (0.10%-50%) exceeding the allowable error of a navigation mark is less than or equal to 5%.

A system provides for an ability to automatically identify one or more chemical components of a sample, especially a biological fluid sample, based on analysis of spectral data by at least one artificial intelligence module. The artificial intelligence module is able to be trained on a plurality of spectral data samples having known concentrations of individual chemicals and elements. The system is preferably used for spectral techniques such as UV / vis and near-infrared (NIR) spectrophotometry, but is also able to be used for other forms of spectroscopic techniques, including mass spectrometry, infrared (IR) spectrometry, X-ray spectroscopy, Raman spectrometry, or nuclear magnetic resonance (NMR) spectrometry.

The invention provides a method for enhancing heavy metal ion concentration analysis and detection sensitivity and application. The method comprises the following steps: S1, carrying out gradient dilution on a heavy metal solution and a zinc ion solution to obtain a dilute solution with a series of concentration gradients; s2, measuring the absorption values of the dilute solutions with the series of concentration gradients by adopting a spectrophotometric method, and establishing a standard curve; according to the method, the zinc ions are introduced as interference variables when the standard curve for measuring the heavy metal ions is constructed, the slope of the standard curve can be remarkably increased by adding the zinc ions, the analysis and detection sensitivity of the spectrophotometric method can be effectively improved, and a new sensitivity optimization strategy is provided for heavy metal ion detection.

The invention relates to the technical field of concentration detection of phosphate, in particular to a method for detecting the concentration of phosphate ions in a nickel-free hole sealing tank solution. The detection method comprises the following steps: adding a nickel-free hole sealing tank solution into a quantitative bottle for quantification, adding an ascorbic acid solution and a molybdate solution into a volumetric flask, carrying out a standing reaction at 28-32 DEG C for 15-25 min, determining the light absorption value of the reaction solution at 700 nm by using a spectrophotometer, obtaining the phosphorus content M according to a phosphorus standard curve model, and calculating the phosphate ion concentration P according to a formula. Compared with an existing method for detecting the concentration of orthophosphoric acid through IC equipment, the concentration data measured through the spectrophotometric method is more stable and smaller in difference, the accuracy of concentration analysis of the phosphate ions in the nickel-free hole sealing groove can be achieved, and the situation that materials are scrapped due to the fact that the concentration of the phosphate ions is abnormal in the production process is effectively avoided.

The application discloses a double-optical-path spectrometer, a color measurement device and a calibration method, and relates to the field of color measurement devices. The color measurement device obtains reflected light of a measured sample and reflected light of a test light source, and inputs the reflected light into a first incident slit and a second incident slit of a spectrometer through a Y-shaped light path unit; the reflected light is incident on a grating through a lens; diffracted light spots returned by the grating are incident on a photosensitive surface of a sensor on the side of the incident slit through the lens; the photosensitive surface is provided with an aperture stop with a dislocation opening; and the photosensitive surface at each dislocation opening only receives light signals of one light path, so that the short-term repeatability of spectrophotometry is improved. When wavelength calibration is performed, light signals of a calibration light source are obtained through the Y-shaped light path unit; pixel position information of the collected light signals is obtained according to a sensor of the spectrometer; a fourth-order polynomial is used to fit wavelength data and the pixel position information; the correspondence between the pixels and the wavelengths is adjusted; and the wavelengths are calibrated, so that the short-term repeatability of spectrophotometry is improved.