The invention belongs to bioenergy source utilizing field, especially relating to a bioreactor-direct microbial fuel cell and its use. And it mainly comprises tubular anode chamber and cathode chamber and proton exchange membrane to isolate the two chambers, where both the electrodes are of unpolished high-purity graphite rod; the anode is inoculated with bacteria, where before inoculation, charging aseptic N2-CO2(80:20) mixed gas to remove all oxygen gas from culture medium and after inoculation, slowly charging mixed gas and keeping strict anaerobic environment; the cathode keeps charging aseptic air and keeps dissolved oxygen concentration in the aseptic air; in the two chambers, slowly blending with magnetic blender; before used, the battery device is processed by high pressure moisture-heat sterilization. And the invention can use glucose, fructose, xylose, sucrose, maltose, and other sugar-containing matters and organic waste water as raw materials and directly converts their chemical energies into electric energy, having characters of no medium toxicity, good stability, moderate operating conditions, clean power generation, etc.