818 results about "Bioreactor" patented technology

The invention discloses a method for constructing a multicellular living body material by adopting a droplet bioreactor, which belongs to the crossing field of biological materials and microbial engineering, and comprises the following steps: (1) resuspending different cells in different polymer solutions to prepare degradable or phase-separable hydrogel particles containing cells; (2) dispersing the hydrogel particles in a cross-linkable hydrogel matrix to form a composite hydrogel; and (3) curing the composite hydrogel, and then carrying out degradation treatment to obtain the liquid cavity structure multicellular system bioreactor with spatial partition and dynamic interaction characteristics. According to the invention, ordered distribution and metabolism synergy of different microorganisms in space can be realized, controllable degradability and excellent mass transfer performance are realized, bacterial slow release and metabolite exchange can be maintained, and the method is suitable for long-term stable operation of a continuous flow reactor. The method has wide application prospects in the fields of green biological manufacturing, renewable energy source production, environmental restoration, biomedical engineering and the like.

The invention relates to the technical field of shellfish breeding, in particular to a rapid microalgae breeding method for shellfish breeding based on a photobioreactor and a cement pool. The method comprises the following steps: firstly, optimizing a photobioreactor structure and an illumination layout, controlling a culture environment by matching with a multi-parameter regulation and control system, breeding microalgae seeds with high resistance and high carbon sequestration capacity, culturing to a preset density, and partially harvesting and inoculating into a cement pond, after partial harvest, the photobioreactor realizes continuous culture by supplementing disinfected seawater and nutritive salt; the harvested high-density microalgae are inoculated into a cement pond, nutritive salt is supplemented in a doubled mode, air and carbon dioxide mixed gas is used for continuous aerated culture, and the microalgae are promoted to rapidly reach the index growth period and used for being directly fed to shellfish larvae; according to the rapid microalgae breeding method for shellfish seedling culture, the yield and quality of microalgae are both considered, the success rate and productivity of aquatic seedling culture are effectively improved, the production cost and the labor intensity of workers are reduced, and the method is suitable for large-scale popularization.

The present disclosure relates to a biodegradable multilayer film comprising stacked on each other in the following order: a) a first outer layer comprising a first polymer selected from the group consisting of poly (butylene adipate-co-terephthalate) (PBAT), poly (butylene succinate) (PBS), poly (butylene succinate-co-adipate) (PBSA), and mixtures thereof; b) a core layer comprising a blend of thermoplastic starch (TPS) and polybutylene adipate terephthalate (PBAT); c) a second outer layer comprising a second polymer selected from the group consisting of poly (butylene adipate-co-terephthalate) (PBAT), poly (butylene succinate) (PBS), poly (butylene succinate-co-adipate) (PBSA), and mixtures thereof. The present disclosure also relates to a disposable bag, a bioreactor and a container suitable for mixing, packaging, storing or transporting biopharmaceutical substances, wherein the disposable bag, bioreactor and container comprise the biodegradable multilayer film.

The invention discloses a recombinant subunit vaccine for resisting trichomonas pigeonae infection and a preparation method of the recombinant subunit vaccine. The vaccine comprises two recombinant proteins with sequences as shown in SEQ ID NO: 7 and SEQ ID NO: 8 respectively and a pharmaceutically acceptable carrier. According to the invention, Sf9 cells are used for respectively expressing trichomonas pigeonae adhesion protein 33 and adhesion protein 65, the immunogenicity of the obtained recombinant protein is similar to that of natural protein, the expression level is high, the immunogenicity is strong, a very small amount of recombinant protein can provide a better immune protection effect, and the recombinant protein has no pathogenicity to pigeons, and can be used for preparing the recombinant protein for preventing and treating trichomonas pigeonae. Meanwhile, large-scale serum-free suspension culture can be carried out through a bioreactor, so that the vaccine production cost is greatly reduced.

Modular mucosal tissue bioreactors combined with bioartificial mucus provide a high-fidelity, medium-throughput platform for evaluating CF treatments and modeling host-pathogen biology, a stepping stone towards better options for HEMT-nonresponsive patients. Whole lung ex vivo Cystic Fibrosis model system allows for investigation of candidate CF therapies.

The invention relates to a recombinant expression cat interferon-omega gene and a preparation method and application thereof, the nucleotide sequence of the cat interferon-omega gene is as shown in SEQ ID NO.1, and the cat interferon-omega gene is obtained by introducing an Fc fusion fragment to the C terminal and / or N terminal of the natural gene sequence of the cat interferon-omega. XTEN or PAS is introduced to prolong a peptide fragment, and original glycosylation modification sites on a natural sequence are reserved. The method comprises the following steps: cloning a cat interferon-omega gene into an expression vector containing double promoters, further transfecting into a cell, carrying out stable cloning and screening, establishing a high-expression cell strain, and carrying out fermentation culture in a bioreactor. According to the invention, the cat interferon-omega gene is subjected to multiple modification and is efficiently expressed in a CHO-K1GS system, so that the protein yield, the stability and the half-life period are remarkably improved; the obtained fusion protein is high in purity and strong in activity, can obviously enhance immune response and protection effect when being matched with cat vaccines, and is good in safety.

The utility model discloses a table type glass bioreactor and relates to the technical field of bioreactors. The bioreactor comprises a bioreactor body, wherein a sealing cover is arranged at the top of the bioreactor body. The moving mechanism can drive the stirring mechanism to rotate while moving up and down, so that the stirring mechanism is in a working state of rotating while moving up and down in the bioreactor body, and high-temperature liquid positioned below the bioreactor body can flow to an upper area; meanwhile, the liquid with lower temperature above the bioreactor body flows to the area with higher temperature below the bioreactor body, so that the temperature gradient of different height positions in the bioreactor body is broken, even in the area far away from the heating plate, the uniform distribution of the temperature can be realized, and the consistency of the temperature in the bioreactor is improved; and biological processes such as cell culture and fermentation are facilitated.

The invention discloses a cat kidney suspension culture cell line suitable for serum-free suspension culture and application of the cat kidney suspension culture cell line. The name of the cell line is F81-B4, and the preservation number is CGMCC (China General Microbiological Culture Collection Center) No.46350. After 50 generations of continuous subculture, the cells still can maintain the original proliferation level and cellular morphology, the cell number can reach 9.0 * 10 < 6 > / mL after the cells are subcultured at the density of 1.0 * 10 < 6 > / mL for three days, the multiplication time is 22-26 hours, and the cell proliferation speed is equivalent to that in a shake flask when the cells are amplified to a 10L bioreactor for culture. The cell line is highly susceptible to feline parvovirus, feline calicivirus, feline herpes virus, canine parvovirus and canine distemper virus, the virus content of a virus solution harvested 72 h after FPV virus inoculation can reach 107.5 TCID50 / mL, the virus content of a virus solution harvested 24 h after FCV virus inoculation can reach 1010.8 TCID50 / mL, the virus content of a virus solution harvested 48 h after FHV-1 virus inoculation can reach 107.5 TCID50 / mL, compared with an existing F81 adherent cell production process, the production process has the advantages that the production efficiency is high, and the production cost is low. The method has the characteristics of rapid lesion, short virus collection time and high titer. The cell matrix is an ideal cell matrix which can be used for culturing the three viruses.

The invention discloses a bioreactor multi-parameter adaptive control system and method based on machine learning, and belongs to the technical field of bioreactor intelligent control, and the bioreactor multi-parameter adaptive control system comprises a bioreactor hepatocyte culture unit, a data acquisition and control unit and a machine learning intelligent decision-making unit. And the data acquisition and control lower computer controls operation variables such as gas supply, sampling and perfusion in the culture unit execution mechanism, collects culture process data returned by the parameter detection module, constructs a data-driven cell state prediction model by utilizing machine learning, and optimizes model parameters and operation variables based on a prediction result to realize closed-loop control. The method can overcome the defect that there is no precise mechanism model for hepatic cell culture, and multi-parameter collaborative optimization can be realized only depending on process data, so that the state of the hepatic cells is sensed in real time, full-cycle optimization of cell viability and functions is realized, and the activity and density of hepatic cell culture are effectively improved.

The invention relates to a method for inhibiting rumen gas production by using a genetically modified laver filament as a ruminant feed additive and a method for inhibiting methane production by using the genetically modified laver filament as the ruminant feed additive. A porphyra yezoensis bromine peroxidase gene is cloned, an overexpression vector of the gene is constructed, porphyra yezoensis thallus is used as a receptor for genetic transformation, and a porphyra algal strain of which the key enzyme expression quantity is remarkably improved is obtained through screening. The method comprises the following steps: inducing thalli to generate protonema, crushing the protonema by using a crusher, carrying out ventilated large-scale culture in a seawater culture medium added with PES by using a column bioreactor to obtain a large number of protonema materials, collecting the protonema materials, crushing the protonema materials by using the crusher, and drying at low temperature. The obtained laver protonema powder is put into a vacuum bag for low-temperature preservation in dark light, and can be applied to preparation of ruminant feed as a dairy cow feed additive. The filamentous can accumulate halogenated compounds such as bromidogen and the like, can inhibit the activity of a key enzyme generated by methane in rumen, and further inhibits the generation of methane.

The embodiment of the invention discloses a method for constructing a cartilage micro-tissue by compounding an extracellular matrix with a vascular matrix component and application. The method comprises the following steps: preparing a porcine decellularized cartilage extracellular matrix; generating a cartilage extracellular matrix suspension; carrying out screening treatment on the cartilage extracellular matrix suspension; preparing a porcine acellular cartilage extracellular matrix microcarrier; preparing a target suspension of a common system from the porcine acellular cartilage extracellular matrix microcarrier and vascular matrix component cells, and transferring the target suspension to a bionic floating microenvironment of a shaker bioreactor for dynamic culture; carrying out culture treatment on the porcine acellular cartilage extracellular matrix microcarrier; and taking out the cell-loaded porcine decellularized cartilage extracellular matrix microcarrier from the cultured and treated porcine decellularized cartilage extracellular matrix microcarrier as the constructed cartilage micro-tissue. According to the embodiment, the condition of secondary injury to a user can be improved and reduced, the repairing effect is improved, and the adaptability of the constructed cartilage micro-tissue is improved.

The invention relates to the technical field of oxygen permeation membranes and sewage treatment, in particular to a method for preparing a PMP braided tube enhanced hollow fiber oxygen permeation membrane through thermally induced phase inversion, the hollow fiber oxygen permeation membrane and application of the hollow fiber oxygen permeation membrane. The PMP braided tube reinforced hollow fiber oxygen permeation membrane has the advantages that the membrane filaments are made to form small and dense micropores in the extraction process, the porosity is improved, the gas flux of the PMP braided tube reinforced hollow fiber oxygen permeation membrane is high, and the PMP braided tube reinforced hollow fiber oxygen permeation membrane has the advantage of high mechanical strength of the membrane filaments, so that the durability is good. The preparation method does not need a single process for defoaming, prepares the primary film by a one-step method, and has the characteristics of short time consumption, high production efficiency and simple and convenient process. The PMP braided tube enhanced hollow fiber oxygen permeation membrane is applied to preparation of membrane aeration bioreactors and sewage treatment, and has good application prospects.

The invention provides an auxiliary operation and maintenance prediction method and device for a biological reaction tank activated sludge treatment process based on a robot, relates to the field of sewage treatment, and solves the technical problem that the early warning capability for abnormal sludge working conditions is insufficient due to the fact that dynamic association between sludge character indexes and effluent quality is not fully considered. The method comprises the following steps: inputting preprocessed water quality data into a water quality parameter prediction channel, and extracting a long-term dependency relationship and a local mode of a water quality parameter sequence from the water quality data through a time sequence convolutional network; and inputting the index data of the pretreated sludge and the image data of the sludge in the biological reaction tank into a sludge character analysis channel, and extracting a bidirectional time sequence dependency relationship and the sensitivity to sequence change through a bidirectional long-short-term memory network. And through a cross attention mechanism in the sludge character and water quality prediction fusion model, carrying out dual-channel feature weighted fusion. The method is used for establishing a sludge character and water quality prediction model and device, and the intelligent operation level of a sewage treatment plant is improved.

A method of microbial biosynthesis of a composite includes subjecting a first culture within a bioreactor to incubation conditions, the first culture including a first bacteria that exhibit production of a structural polysaccharide, to thereby produce an organic network structure made of produced structural polysaccharide. A second culture is added to the bioreactor after the organic network structure is produced, the second culture including a second bacteria that exhibit mineralization of calcium carbonate via microbial-induced carbonate precipitation. The second bacteria produce calcium carbonate particles which precipitate on the organic network structure to produce a mineralized organic network structure as the composite. The calcium carbonate particles can be chemically converted to calcium hydroxyapatite particles.

1. Name of the design product: bioreactor (2L). 2. Use of the design product: for plant cell expansion, cell therapy, antibody drug cell expansion. 3. Design points of the design product: in shape. 4. Picture or photo best indicating the design points: perspective view 1.

This invention provides a method for propagating pluripotent stem cells (PSCs) by suspension culture in a bioreactor. [Solution] The method comprises the following steps: (i) adding a ROCK inhibitor (ROCKi) to pluripotent stem cells cultured in suspension in a bioreactor; (ii) adding a cell dissociation agent, thereby dissociating aggregates of pluripotent stem cells; (iii) diluting the cell dissociation agent added in step (ii) by adding a sufficient excess volume of culture medium to reduce the concentration of the cell dissociation agent to a concentration at which cell aggregates can be re-formed; and (iv) culturing the mixture obtained in step (iii) under appropriate conditions that allow for the proliferation of PSCs.

This invention discloses a wastewater treatment bioreactor, primarily relating to the technical field of bioreactors. It includes a reaction tank with an inlet and an outlet at the bottom and top, respectively. An aeration tank is located at the top of the reaction tank, containing several aeration pipes. A biofilm is located at the bottom of the aeration tank. The tops of the aeration pipes pass through the reaction tank and connect to it. An air inlet is located on the aeration tank. A movable frame is vertically slidably connected inside the reaction tank, and several rotating blocks, fitted around the aeration pipes, are rotatably connected to the movable frame. Several first brushes, which cooperate with the aeration pipes, are located on the inner sides of the rotating blocks. The beneficial effects of this invention are: solving the problem of membrane pores being easily clogged, hindering oxygen transfer; improving the oxygen transfer efficiency and aeration effect of the membrane module; improving pollutant removal efficiency; and ensuring the quality of the reactor effluent.

This bioreactor is provided with: a fixed cylindrical surface centered on an axis line extending in the vertical direction; a rotary cylindrical surface which extends around the axis line, partitions and forms a pool, the pool serving as a culturing zone, between the rotary cylindrical surface and the fixed cylindrical surface that face each other in the radial direction of the axis line, and is rotatable around the axis line; a drive part which rotationally drives the rotary cylindrical surface around the axis line; a bottom plate part which partitions the pool from below and forms a bottom surface extending uniformly in the circumferential direction; and an air diffusion part which supplies, from the bottom surface upward, air bubbles formed below the bottom surface.

The utility model relates to the technical field of desulfurization equipment, in particular to a biological desulfurization device for recovering elemental sulfur, which is characterized in that a gas-liquid distributor is arranged above a bioreactor, a sulfur precipitation tank is arranged below the bioreactor, and raw materials uniformly enter the bioreactor after passing through the gas-liquid distributor; and elemental sulfur particles generated by biological reaction enter a sulfur precipitation tank. The device disclosed by the utility model has the beneficial effects that high-efficiency recovery of sulfur and stable operation of the system are realized through gas-liquid distribution optimization, carrier immobilized microorganisms and the high-efficiency sulfur separation module.

The utility model discloses a three-phase circulation bioreactor. The three-phase circulation bioreactor comprises a shell, a communicated exhaust pipe is fixedly connected to the center of the top of the shell; a communicated overflow pipe is fixedly connected to the side wall of the top of the shell; a blow-off pipe and a water inlet pipe which are communicated with each other are fixedly connected to the periphery of the bottom of the shell; a net plate is fixedly connected to the middle of the interior of the shell; a biofilm carrier filler is arranged at a position, corresponding to the upper part of the screen plate, in the shell; the gas escapes upwards, so that the gas returns to the upper part of the water body after reaching the bottom of the sewage, the circulation effect is achieved, and the mixing effect of the gas and the sewage is further improved; besides, the spray heads can rotate along with the hollow shaft, and the spray heads on the different vortex-shaped pipes are arranged in a staggered manner, so that the distribution density of the gas in the sewage water body can be improved, the mixing effect between the sewage and the gas is further improved, the biological treatment reaction speed of the sewage is prevented from being influenced, and the purification efficiency of the reactor is improved.

To provide a cell proliferation system, proliferation method, control method, and culture medium bag capable of proliferating various adherent cells and suspension cells. [Solution] Cells are grown in a bioreactor and activated by an activator (e.g., a soluble activator complex). The nutrient supply and gas exchange functions of an automated closed cell proliferation system make it possible to seed at, for example, a low seeding density. By manipulating the parameters of the cell growth environment, cells are placed in specific locations within the bioreactor for efficient exchange of nutrients and gases. System parameters are adjusted to shear any cell colonies that may form during the proliferation phase. Metabolic concentrations are controlled to improve cell growth and viability. Cell retention within the bioreactor is controlled. In some embodiments, the cells include T cells. In other embodiments, the cells include a T cell subpopulation, for example, regulatory T cells (Tregs).

A method for producing mycelium biomass. The method comprises obtaining a base media composition and an antifoam solution; sterilizing a mixture of the base media composition and the antifoam solution

A stirring method includes: stirring cell suspension by circulating the cell suspension in a circulation path including a bioreactor; acquiring a predetermined or selected physical quantity related to the cell suspension at a measurement target site in the circulation path over time; and determining if a uniform diffusion state in which cells are uniformly diffused in the cell suspension is reached by comparing a degree of variation of the physical quantity to the a predetermined or selected degree.

The invention discloses a fuzzy intelligent control method and device for a pichia pastoris fermentation process. The fuzzy intelligent control method comprises the following steps: (1) integrating an online sensor in a bioreactor and monitoring state parameters of fermentation liquor in real time; (2) inputting the monitored parameters into a central control system, and processing the parameters through a fuzzy intelligent control algorithm; and (3) according to an output instruction of the fuzzy intelligent control algorithm, cooperatively regulating and controlling fermentation operation parameters in real time. According to the method disclosed by the invention, a fuzzy intelligent control algorithm is developed by monitoring dissolved oxygen, relative concentration of dissolved carbon dioxide, methanol concentration and pH in fermentation liquor in the fermentation process in real time, so that the fermentation process is accurately regulated and controlled by multi-parameter coordination, and the method is applied to process regulation and control of pichia pastoris fermentation expression recombinant protein. The expression quantity and the production efficiency of the recombinant albumin can be remarkably improved, the production period is shortened, and the method is suitable for industrial large-scale production.

The present application relates to a kind of wastewater treatment system and processing method of electrolytic water anode oxygen coupling biological technology, belong to sewage treatment technical field, solve the technical problems such as the micro-aerobic sewage biological treatment technology needs accurate dissolved oxygen, organic matter remains, total nitrogen removal efficiency needs to be improved, including electrolytic water device, including polymer membrane electrolytic cell, proton exchange membrane is arranged in polymer membrane electrolytic cell, proton exchange membrane is used to divide polymer membrane electrolytic cell into cathode chamber and anode chamber, pure water is used to be introduced in cathode chamber, and the wastewater to be treated is used to be introduced in anode chamber;The wastewater inlet of anode chamber is connected with bioreactor, and the wastewater outlet of anode chamber is connected with bioreactor.In electrolysis process, oxygen evolution reaction occurs in anode, and the dissolved oxygen generated is immediately mixed with wastewater, enters bioreactor, improves the degradation efficiency of organic matter, ammonia nitrogen and other pollutants, while avoiding the disadvantages such as high energy consumption and low oxygen utilization rate caused by traditional air blowing aeration of bioreactor.

The application discloses a kind of folded plate type anaerobic biological reactors, including box, the water inlet and water outlet are equipped in two opposite side walls of the box;Box is sequentially provided with at least two reaction compartments, the top of upstream reaction compartment is communicated with the lower part of downstream reaction compartment, the water inlet is communicated with the lower part of first reaction compartment of head end, the water outlet is communicated with the top of last reaction compartment of terminal end;The side wall of the box above the water outlet is further provided with exhaust port;Each reaction compartment is respectively provided with biological filler module, and the biological filler module includes polyurethane foam filler.The application further discloses an application of the folded plate type anaerobic biological reactor in treating aquaculture wastewater.The technical scheme of the application has the remarkable effects of significantly improving oxygen resistance, outstanding archaea enrichment effect, double improvement of treatment efficiency and biogas quality, and significant resource value.

Bioreactors and associated methods are provided herein including bioreactors capable of treating water contaminated with 1,4-dioxane. In certain embodiments, a bioreactor is disclosed and may include an adsorbent layer with a biofilm capable of metabolizing 1,4-dioxane and a screen disposed downstream of the adsorbent layer configured to retain detached biofilm.

This invention provides a distributed microalgae photobioreactor and a method for cultivating microalgae. The reactor includes an environmental control chamber, which is at least partially transparent to light. A rotating support frame is located within the environmental control chamber and is connected to a rotation drive mechanism. The rotating support frame supports multiple independent cultivation containers, which are used to hold culture medium and microalgae respectively. The rotation mechanism drives the rotating support frame to rotate, thereby causing the multiple cultivation containers to rotate. The technical solution of this invention achieves physical isolation through independent cultivation containers, ensuring high aseptic reliability. It also achieves efficient mixing, low energy consumption, high and directionally controllable supplemental lighting, and has a wide range of applications and strong practicality.

A method for organic compound removal from a solution using microalgae includes extracting one or more hydrocarbons from an underground geologic formation to form a hydrocarbon composition and a produced water including at least a portion of the one or more hydrocarbons and one or more salts. The method further includes mixing the produced water with a wastewater to form a solution, and then contacting a microalgae feedstock, Chlorella sorokiniana, with the solution in a bioreactor. The total lipid content of the microalgae feedstock is 13 to 17%. The method includes removing the one or more hydrocarbons, nitrogen, and phosphorous from the solution by algal consumption with the microalgae feedstock. The method of the present disclosure removes 80 to 90 wt. % of the one or more hydrocarbons, 70 to 85 wt. % nitrogen, and 90 to 100 wt. % phosphorous.