2515 results about "Bioreactor" patented technology

The present disclosure relates to systems, devices, and methods for automated cell processing within a cell processing system. In an embodiment, the present disclosure relates to a cartridge comprising a bioreactor module comprising a bioreactor, a first thermal compartment positioned adjacent the bioreactor, and a second thermal compartment positioned adjacent the bioreactor, and a fluidic manifold coupling the first and second thermal compartments to the bioreactor.

The invention provides a virtual-real fusion method for digital twinning operation of a cell drug factory in combination with the Internet of Things, which comprises the following steps: constructing geometric models of a culture medium storage tank and a bioreactor through three-dimensional modeling software, mapping and associating historical process parameters of a culture medium supply system and temperature, pH value and dissolved oxygen concentration monitored in real time with the geometric models, and obtaining a virtual-real fusion model of the culture medium storage tank and the bioreactor; constructing a digital twinborn model of the reactor; analyzing historical production data through a machine learning algorithm, extracting a quantitative relationship among key parameters of a cell metabolism rate, a glucose concentration in a culture medium, a pH value and a reactor temperature, establishing a cell growth prediction model, and predicting a growth trend and a metabolism demand of cells in a target time period; a virtual-real mapping mechanism of a culture medium supply system is integrated with an intelligent manufacturing execution system, so that the culture medium supply process and the whole pharmaceutical production process are subjected to collaborative optimization control, and the working procedures of the culture medium production process are automatically dispatched according to a production plan and cell growth requirements.

The invention belongs to the technical application field of microbial metabolic engineering and synthetic biology, and particularly relates to a pichia pastoris engineering strain for high-yield sclareol as well as a construction method and application of the pichia pastoris engineering strain. The method comprises the following steps: constructing a biosynthetic pathway of sclareol in a host strain, and optimizing an intracellular mevalonic acid metabolic pathway and a central metabolic pathway to obtain an engineering strain A; the host strain is pichia pastoris; or overexpressing or knocking out a metabolism regulation factor in the obtained engineering bacterium A to obtain an engineering bacterium strain B; or, in the obtained engineering bacterium B, a synthetic pathway is targeted to peroxisome and optimized by adopting a cell region compartment strategy, and an engineering bacterium strain C is obtained. The invention provides a pichia pastoris chassis cell for synthesizing a diterpenoid sclareol and an engineering strain for synthesizing sclareol. By introducing and optimizing the expression of a sclareol synthesis pathway, the fermentation yields of sclareol in shake flask batch and bioreactor batch fed-batch fermentation are respectively 631.6 mg / L and 10.5 g / L.

The present disclosure relates to systems, devices, and methods for automated cell processing within a cell processing system. In an embodiment, the present disclosure relates to a cartridge comprising a bioreactor module comprising a bioreactor, a first thermal compartment positioned adjacent the bioreactor, and a second thermal compartment positioned adjacent the bioreactor, and a fluidic manifold coupling the first and second thermal compartments to the bioreactor.

A system for growing cells comprising a bioreactor chamber for growing the cells, a delivery system delivering a perfusion solution to the bioreactor chamber for perfusion of the perfusion solution through the cells, a dialysis system having a dialyzer, a dialysate for performing a dialysis and a filter for reducing ammonia content in said dialysate, and a controller that circulates the perfusion solution through the dialyzer and the dialysate through the filter.

The invention relates to the technical field of sewage treatment and automatic control thereof, in particular to an automatic monitoring system and method for sewage treatment of a vibrating membrane bioreactor. The method comprises the following steps: monitoring key indexes in real time, and carrying out standardization processing and interpolation on sensor data to ensure the accuracy of the data; an information code generation and verification mechanism is adopted to ensure data integrity and security; a physical model and a deep learning model are combined to predict sewage treatment efficiency, and space-time relevance is analyzed through a space-time convolutional network; operating parameters of the system are optimized through a deep reinforcement learning algorithm, and the frequency, the amplitude, the aeration power and the medicament dosage of a vibrating membrane are adjusted to achieve an optimal treatment effect; through real-time adjustment of the vibrating membrane and the aeration equipment, the sewage treatment efficiency is improved; the real-time treatment state is displayed, the sewage removal rate is monitored, and an alarm mechanism is triggered under the abnormal condition. According to the method, the data security and the system optimization are considered while the processing effect is ensured.

The invention discloses a bioreactor process optimization system and method, and relates to the technical field of bioreactors. Comprising the following steps: collecting historical reaction process data of a bioreactor, and obtaining real-time reaction process data of the bioreactor; analyzing the historical reaction process data to obtain different influence results of different adjustment modes on the biological reaction process; establishing a cell culture model according to a corresponding relation obtained by different influence results of different adjustment modes on the biological reaction process; the real-time reaction process data is obtained, the biological culture process is influenced by adjusting the bioreactor, the specific adjustment mode is obtained through the real-time reaction process data and the required adjustment result, the required adjustment result can be obtained after the reaction in the adjustment mode obtained through backstepping, hysteresis is avoided, materials are saved, and the efficiency is improved. And the problem of poor subsequent biological reaction caused by excessive material addition, excessive design parameter adjustment and poor subsequent biological reaction due to hysteresis is also avoided.

A bioreactor systems and processes for the production of biomass are provided. The systems comprise: at least one bioreactor unit which comprises at least one liquid-containable compartment. The liquid- containable compartment comprises, (i) a first wall, wherein the first wall comprises a composite membrane that is permissible to transfer of gases therethrough, wherein the composite membrane comprises at least one barrier layer and at least one reinforcement layer; and (ii) a second wall, wherein the second wall comprises material that is optically transmissible to visible light and that is substantially less gas permeable than the first wall, wherein the first and second walls cooperate to define the liquid-containable compartment within the bioreactor unit.

The invention relates to the technical field of sewage purification, in particular to an environment-friendly sewage purification system which comprises a pretreatment unit, a detection unit, an analysis unit, a reaction unit, a cleaning unit, an aeration unit and a recovery unit. The detection unit detects the flow of the water inlet and outlet, the water inlet and outlet pressure of the bioreactor and the water level of the reaction tank; the analysis unit is used for adjusting the flow of the water inlet, judging the running state of the membrane biological component, adjusting the backwashing period, determining each pressure difference curve section, judging the pollution degree of sewage and adjusting the aeration mode of the aeration unit; the reaction unit comprises a water pump, an anaerobic tank, a reaction tank and a plurality of membrane biological components; the cleaning unit periodically performs reverse cleaning on the membrane biological component; and the aeration unit introduces air into the sewage according to the determined working mode. According to the invention, efficient solid-liquid separation is carried out on sewage, such that a disadvantage that sludge bulking easily occurs in a traditional activated sludge method is overcome.

The present disclosure provides methods and systems for production of biostimulants that promote nitrogen use efficiency in plants. Embodiments described include methods of making a biostimulant composition in a bioreactor system that includes two or more containers arranged in series. The bioreactor system may include an established population of a nitrogen use efficiency-promoting microbial strain. The method may include operating the bioreactor system by transferring into the system an aqueous feedstock that comprises a microbial consortium, transferring working fluid between containers of the system, and collecting a product. The method may further include maintaining a concentration of a nitrogen use efficiency-promoting microbial strain in the system.

To provide a manufacturing system and method for creating multiple tissue constructs from cells.SOLUTION: A system can include a thaw subsystem (if the cells are provided in a frozen state), an expansion subsystem, a concentration subsystem, and a tissue maturation subsystem. Each of these subsystems is modular and can be reconfigured, and a process can be repeated depending on the specific tissue process being implemented. Multiple tissue types can be combined in multiple bioreactors. The activities of multiple bioreactors can be coordinated and controlled in an automated manner by a supervisor controller. The supervisor controller can receive user input at the start of the process, and can manage the process thereafter, alerting the user if user actions are required.SELECTED DRAWING: None

The invention relates to the technical field of sewage treatment, and discloses an integrated sewage treatment method based on intelligent sensing. According to the method, sewage flow, PH value, temperature, turbidity and suspended matter concentration are collected in real time through an intelligent sensor, in each treatment link, the intelligent sensor can monitor the concentration of organic matter, nitrogen, phosphorus and other pollutants, the pollutant removal rate and the biological reaction rate are calculated, timely adjustment of a sewage treatment strategy is achieved, and the sewage treatment efficiency is improved. Through real-time monitoring of dissolved oxygen, temperature and mixed liquid reflux ratio in the bioreactor, reaction conditions are optimized, biodegradation efficiency and sludge yield are improved, and through monitoring of the dosage and reaction time in the reaction process, the dosage adding proportion is optimized, so that the removal effect of chemical reaction is improved; finally, the flow, the PH value, the chemical oxygen demand and the biological oxygen demand of the treated effluent are comprehensively monitored, so that the sewage treatment efficiency and reliability are remarkably improved.

The invention provides an automatic stirring device in the field of fluid machinery and bioreactors, focusing on enhancing fluid control efficiency and consistency in biological cultivation processes. It utilizes a flexible channel body or reactor body to facilitate the flow of nutrient solution along specific channels (such as S-shaped flexible channels or S-shaped loop channels), designed to reduce shear forces and achieve iso-level uniform flow. A power system and monitoring and control system are employed to regulate the flow of nutrient solution and monitor cultivation conditions in real-time, thus supporting various bioprocess cultivation requirements.

The utility model discloses a magnetic aeration stirring device, which comprises a stirring disc circumferentially rotating around the axis of the stirring disc, the stirring disc comprises a paddle assembly and a magnetic rotor assembly, and the rotation of the stirring disc is realized through the magnetic coupling effect between the magnetic rotor assembly and an external driving assembly; the annular aeration disc is arranged on the outer side of the stirring disc in a surrounding mode, the annular aeration disc and the stirring disc are arranged in a split mode, and an air outlet part is arranged on the annular aeration disc; the connecting film is respectively fixed with the stirring disc and the annular aeration disc, so that the stirring disc is hermetically connected with the annular aeration disc through the connecting film. According to the technical scheme, the annular aeration disc and the stirring disc are separately arranged, so that the annular aeration disc and the stirring disc can be independently manufactured, the manufacturing cost is reduced, parts of various models can be conveniently assembled and matched, and the suitability is improved.

The invention provides a mesenchymal stem cell culture monitoring system based on multiple parameters, and relates to the technical field of data processing, and the mesenchymal stem cell culture monitoring system comprises a first-level control instruction generation module which is used for generating a second-level control instruction when the metabolism-morphological abnormality association intensity in a combined fuzzy state matrix is judged to reach a preset association threshold value through a fuzzy rule base; when the abnormal state flag bit indicates that the isolated metabolism is abnormal, a second-level control instruction is generated; the driving unit is used for responding to the first-stage control instruction and cooperatively driving the buffer solution injection unit and the nutrition supplementing pump; responding to the secondary control instruction, and dynamically adjusting the opening degrees of a stirring rate controller, a temperature feedback loop and a gas proportional valve of the bioreactor. The control efficiency and the cell culture stability are improved.

An anaerobic bioreactor with electrolytic regeneration system, method and apparatus for monitoring pH levels which is simultaneously efficient in both anaerobic digestion and optimized filtration process is disclosed. To maintain the pH stability of an anaerobic bioreactor, an electrolysis process is integrated with the bioreactor. The integration of an electrolysis process with the bioreactor sustains the pH in the optimized range for anaerobic process while enhancing the removal of contaminants in the bioreactor, augmenting biogas production, and mitigating fouling.

A bioreactor for suspended cells is provided. The bioreactor for suspended cells comprises: a housing which is formed in the shape of a container having a culture space with a predetermined volume, and which comprises pots for seeding; a support assembly comprising a plurality of supports arranged at intervals in multiple stages in one direction of the housing; at least one gas inlet penetrating the housing by a predetermined area; and a porous member for covering the gas inlet, wherein the culture space comprises a medium storage space filled with a predetermined amount of a medium so that all the plurality of supports are submerged therein, and the medium storage space can comprise a plurality of cell growth spaces divided through the plurality of supports arranged at intervals in multiple stages.

The invention relates to the technical field of biological medicine, in particular to a hepatitis A inactivated vaccine and a preparation method thereof. Comprising the following steps: cell amplification, virus culture, virus harvesting, cell disruption, ultrafiltration concentration, inactivation, purification and finished product obtaining. The virus culture comprises the following steps: separating from excrement of a hepatitis A patient and carrying out adaptive passage to obtain a virus strain; adding a carrier into a bioreactor, sterilizing for later use, inoculating a virus strain into a collected cell suspension, and transferring into the bioreactor for culturing; the cell disruption step comprises the following steps: carrying out ultrasonic oscillation on a collected virus harvesting liquid to obtain a cell disruption liquid; trichloromethane is added for extraction and centrifugation, and supernatant virus liquid is collected; the invention provides a preparation method of a hepatitis A inactivated vaccine, which is used for preparing the efficient, safe and stable hepatitis A inactivated vaccine by optimizing virus culture and inactivation processes. The method has the advantages of high virus yield and low production cost, and is suitable for large-scale production and application.

The present invention belongs to the technical field of bioreactors, and in particular relates to a high-density culture method for porcine myoblasts based on a bioreactor. Through the steps of resuscitating porcine myoblasts, cell-microcarrier attachment treatment, bioreactor stirring mode conversion, counting and centrifugal cell collection, the porcine myoblasts are difficult to reach a high density in a bioreactor, and a harvest density of 10^6 cells / mL is achieved in the bioreactor, meeting the requirements of cell culture meat production on cell yield; at the same time, the culture time is shortened by more than 20%, greatly saving energy consumption cost and time cost.

The present disclosure provides methods and systems for production of biostimulants that promote nitrogen use efficiency in plants. Embodiments described include methods of making a biostimulant composition in a bioreactor system that includes two or more containers arranged in series. The bioreactor system may include an established population of a nitrogen use efficiency-promoting microbial strain. The method may include operating the bioreactor system by transferring into the system an aqueous feedstock that comprises a microbial consortium, transferring working fluid between containers of the system, and collecting a product. The method may further include maintaining a concentration of a nitrogen use efficiency-promoting microbial strain in the system.

The invention relates to a cell culture system (1) comprising: A bioreactor (100), comprising a vessel (110) and a recycling outlet (120), said recycling outlet (120) being positioned in the vessel (110) so as to be able to extract, from the vessel (110), a multiphase flow comprising a liquid phase and a gas phase; a separator (300) receiving the multiphase flow, said separator (300) being configured to accommodate and maintain a multiphasic system, comprising both the liquid phase and the gas phase; and a recycled media transfer line (220) adapted for routing of at least a portion of the liquid phase from the separator (300) to the bioreactor (100). The invention also relates to a cell culture method (1000) using a cell culture system (1) of the invention and cell biomass obtained thereof.

The invention relates to a method for producing notoginsenoside and ginsenoside by utilizing panax notoginseng cotyledon embryos, and belongs to the technical field of plant adventitious root tissue culture. The method comprises the following steps: inducing embryogenic calluses by utilizing immature zygotic embryos of panax notoginseng; proliferating the panax notoginseng embryogenic callus on a plate culture medium; s2, putting the pseudo-ginseng embryogenic callus obtained in the step S2 into a liquid culture medium, carrying out cell dispersion, and sieving by using a sieve with the aperture of 24-30 meshes; a mechanical stirring type bioreactor is filled with a liquid culture medium, and the globular embryo and the heart-shaped embryo are placed in the mechanical stirring type bioreactor to be cultured; s3, recovering the filtrate in the step S3, filtering by using a screen with the aperture of 30-80 meshes and 80-150 meshes to obtain uniform panax notoginseng embryogenic cells, and differentiating the panax notoginseng embryogenic cells in a triangular flask for the step S4; and drying the cotyledon embryo obtained in the step S4 at 45 DEG C, and grinding into powder. According to the method, large-scale generation of cotyledon embryos can be completed only in 4-6 weeks, the production process is remarkably accelerated, the method is suitable for industrial batch production requirements, and the production efficiency is high.

The present disclosure relates to systems, apparatuses, and methods for sterilizing a closed bioreactor system utilizing superheated water. In particular, in one or more implementations, the disclosed methods include circulating a heated fluid within at least one pressure vessel of a bioreactor system at a pressure above an atmospheric pressure and a temperature above an atmospheric boiling point for the heated fluid. In some implementations, in response to circulating superheated water within a closed bioreactor system for a predetermined dwell time, the disclosed methods include reducing a pressure within the closed bioreactor system to cause the superheated water to evaporate therein. Also, in one or more implementations, the disclosed systems include a combination superheated water and steam generator, a superheated water storage tank, and / or a stratified energy storage tank for utilization in sterilization-in-place and other procedures for producing comestible cell-based food products.

A fluid filtration system includes a fluid storage vessel such as a bioreactor, a filter housing including a filter element disposed therein, a pump coupled between the fluid storage vessel and the filter housing, and a flow diverter disposed between the pump and the filter housing. The pump is configured to move fluid from the fluid storage vessel through the filter element, and the flow diverter is configured for selectively directing fluid received from the pump to the first or second end of the filter housing. In a first mode of operation, the flow diverter directs flow through the filter housing in a first direction, while in a second mode of operation, the flow diverter directs flow through the filter housing in a second direction opposite the first direction.

A perfusion bioprocessing system (10) includes a bioreactor (12) and a recirculation flow path (14) provided with at least in one first feed flow control device (46) and at least one second feed control device (48). The perfusion bioprocessing system (10) further includes a first tangential flow filter (16) coupled to the bioreactor (12) via the recirculation flow path (14) and a second tangential flow filter (18) coupled to the bioreactor (12) via the recirculation flow path (14). The first tangential flow filter and the second tangential flow filter are coupled to a permeate flow path and a retentate flow path. Additionally, the perfusion bioprocessing system (10) includes a control unit (90) coupled to the at least one first feed flow control device (38) and the at least one second feed control device (40).

The invention discloses a method for constructing a multicellular living body material by adopting a droplet bioreactor, which belongs to the crossing field of biological materials and microbial engineering, and comprises the following steps: (1) resuspending different cells in different polymer solutions to prepare degradable or phase-separable hydrogel particles containing cells; (2) dispersing the hydrogel particles in a cross-linkable hydrogel matrix to form a composite hydrogel; and (3) curing the composite hydrogel, and then carrying out degradation treatment to obtain the liquid cavity structure multicellular system bioreactor with spatial partition and dynamic interaction characteristics. According to the invention, ordered distribution and metabolism synergy of different microorganisms in space can be realized, controllable degradability and excellent mass transfer performance are realized, bacterial slow release and metabolite exchange can be maintained, and the method is suitable for long-term stable operation of a continuous flow reactor. The method has wide application prospects in the fields of green biological manufacturing, renewable energy source production, environmental restoration, biomedical engineering and the like.

The invention relates to the technical field of shellfish breeding, in particular to a rapid microalgae breeding method for shellfish breeding based on a photobioreactor and a cement pool. The method comprises the following steps: firstly, optimizing a photobioreactor structure and an illumination layout, controlling a culture environment by matching with a multi-parameter regulation and control system, breeding microalgae seeds with high resistance and high carbon sequestration capacity, culturing to a preset density, and partially harvesting and inoculating into a cement pond, after partial harvest, the photobioreactor realizes continuous culture by supplementing disinfected seawater and nutritive salt; the harvested high-density microalgae are inoculated into a cement pond, nutritive salt is supplemented in a doubled mode, air and carbon dioxide mixed gas is used for continuous aerated culture, and the microalgae are promoted to rapidly reach the index growth period and used for being directly fed to shellfish larvae; according to the rapid microalgae breeding method for shellfish seedling culture, the yield and quality of microalgae are both considered, the success rate and productivity of aquatic seedling culture are effectively improved, the production cost and the labor intensity of workers are reduced, and the method is suitable for large-scale popularization.

The invention discloses a water treatment device of an MABR membrane aeration bioreactor and a treatment method of the water treatment device, and relates to the field of sewage treatment.The water treatment device comprises a vertical frame body, oxygen permeation membranes are evenly arranged in the vertical frame body, and first communicating pipes are arranged at the front ends and the rear ends of the oxygen permeation membranes; the surface of the first communicating pipe at the rear end fixedly communicates with a first conveying pipe, the first communicating pipe is used for conveying oxygen to the oxygen permeation membrane, the first communicating pipe is connected with the vertical frame body through a movement assembly, and through double-form switching of the movement assembly, the oxygen permeation membrane is driven to conduct cyclic twisting and swinging, so that the oxygen permeation membrane is driven to conduct oxygen permeation. The contact of oxygen between the surface of the oxygen-permeable membrane and the microbial biological membrane is enhanced, the oxygen utilization rate is increased, the motion state of the membrane is dynamically adjusted, the self-cleaning capacity of the membrane is enhanced, and sludge deposited on the outer surface of the oxygen-permeable membrane is digested while the acceleration mechanism continuously swings at the bottom of the oxygen-permeable membrane to accelerate the contact of the oxygen-permeable membrane and pollutants in water.

The invention discloses a self-gas-stirring partition plate type efficient anaerobic bioreactor and a method, and belongs to the field of nitrogen and sulfur-containing organic wastewater biological treatment devices. The reactor sequentially comprises a first reaction zone, a second reaction zone, a third reaction zone and a three-phase separation zone which are communicated with one another from bottom to top. Anaerobic granular sludge is inoculated in the first reaction zone for hydrolytic acidification reaction; anaerobic activated sludge with denitrification and sulfate reduction functions is inoculated in the second reaction area, and nitrate and sulfate reduction reaction is carried out. Anaerobic activated sludge with a methanogenesis function is inoculated in the third reaction area, and a methanogenesis reaction is carried out. The three reaction zones are separated by ecological partition plates to ensure water and air permeability and no mud permeability. According to the reactor, the ecological partition plates are arranged to realize anaerobic reaction function division, and multiple components in complex organic wastewater are synergistically removed, so that the pollutant removal efficiency is improved; the flow state in the reactor is improved through self-produced gas backflow, the mass transfer and detoxification effects are enhanced, and the decontamination effect and stability of the reactor are improved.

1. The name of the product of this design: bioreactor. 2. Purpose of the product of this design: bioreactor for culturing cells. 3. The design key point of this appearance design product lies in the shape. 4. The picture or photo that best illustrates the design points: three-dimensional picture.