1769 results about "Bioreactor" patented technology

The invention provides a virtual-real fusion method for digital twinning operation of a cell drug factory in combination with the Internet of Things, which comprises the following steps: constructing geometric models of a culture medium storage tank and a bioreactor through three-dimensional modeling software, mapping and associating historical process parameters of a culture medium supply system and temperature, pH value and dissolved oxygen concentration monitored in real time with the geometric models, and obtaining a virtual-real fusion model of the culture medium storage tank and the bioreactor; constructing a digital twinborn model of the reactor; analyzing historical production data through a machine learning algorithm, extracting a quantitative relationship among key parameters of a cell metabolism rate, a glucose concentration in a culture medium, a pH value and a reactor temperature, establishing a cell growth prediction model, and predicting a growth trend and a metabolism demand of cells in a target time period; a virtual-real mapping mechanism of a culture medium supply system is integrated with an intelligent manufacturing execution system, so that the culture medium supply process and the whole pharmaceutical production process are subjected to collaborative optimization control, and the working procedures of the culture medium production process are automatically dispatched according to a production plan and cell growth requirements.

A system for growing cells comprising a bioreactor chamber for growing the cells, a delivery system delivering a perfusion solution to the bioreactor chamber for perfusion of the perfusion solution through the cells, a dialysis system having a dialyzer, a dialysate for performing a dialysis and a filter for reducing ammonia content in said dialysate, and a controller that circulates the perfusion solution through the dialyzer and the dialysate through the filter.

The invention relates to the technical field of sewage treatment and automatic control thereof, in particular to an automatic monitoring system and method for sewage treatment of a vibrating membrane bioreactor. The method comprises the following steps: monitoring key indexes in real time, and carrying out standardization processing and interpolation on sensor data to ensure the accuracy of the data; an information code generation and verification mechanism is adopted to ensure data integrity and security; a physical model and a deep learning model are combined to predict sewage treatment efficiency, and space-time relevance is analyzed through a space-time convolutional network; operating parameters of the system are optimized through a deep reinforcement learning algorithm, and the frequency, the amplitude, the aeration power and the medicament dosage of a vibrating membrane are adjusted to achieve an optimal treatment effect; through real-time adjustment of the vibrating membrane and the aeration equipment, the sewage treatment efficiency is improved; the real-time treatment state is displayed, the sewage removal rate is monitored, and an alarm mechanism is triggered under the abnormal condition. According to the method, the data security and the system optimization are considered while the processing effect is ensured.

To provide a manufacturing system and method for creating multiple tissue constructs from cells.SOLUTION: A system can include a thaw subsystem (if the cells are provided in a frozen state), an expansion subsystem, a concentration subsystem, and a tissue maturation subsystem. Each of these subsystems is modular and can be reconfigured, and a process can be repeated depending on the specific tissue process being implemented. Multiple tissue types can be combined in multiple bioreactors. The activities of multiple bioreactors can be coordinated and controlled in an automated manner by a supervisor controller. The supervisor controller can receive user input at the start of the process, and can manage the process thereafter, alerting the user if user actions are required.SELECTED DRAWING: None

The invention relates to the technical field of sewage treatment, and discloses an integrated sewage treatment method based on intelligent sensing. According to the method, sewage flow, PH value, temperature, turbidity and suspended matter concentration are collected in real time through an intelligent sensor, in each treatment link, the intelligent sensor can monitor the concentration of organic matter, nitrogen, phosphorus and other pollutants, the pollutant removal rate and the biological reaction rate are calculated, timely adjustment of a sewage treatment strategy is achieved, and the sewage treatment efficiency is improved. Through real-time monitoring of dissolved oxygen, temperature and mixed liquid reflux ratio in the bioreactor, reaction conditions are optimized, biodegradation efficiency and sludge yield are improved, and through monitoring of the dosage and reaction time in the reaction process, the dosage adding proportion is optimized, so that the removal effect of chemical reaction is improved; finally, the flow, the PH value, the chemical oxygen demand and the biological oxygen demand of the treated effluent are comprehensively monitored, so that the sewage treatment efficiency and reliability are remarkably improved.

The invention provides an automatic stirring device in the field of fluid machinery and bioreactors, focusing on enhancing fluid control efficiency and consistency in biological cultivation processes. It utilizes a flexible channel body or reactor body to facilitate the flow of nutrient solution along specific channels (such as S-shaped flexible channels or S-shaped loop channels), designed to reduce shear forces and achieve iso-level uniform flow. A power system and monitoring and control system are employed to regulate the flow of nutrient solution and monitor cultivation conditions in real-time, thus supporting various bioprocess cultivation requirements.

The invention provides a mesenchymal stem cell culture monitoring system based on multiple parameters, and relates to the technical field of data processing, and the mesenchymal stem cell culture monitoring system comprises a first-level control instruction generation module which is used for generating a second-level control instruction when the metabolism-morphological abnormality association intensity in a combined fuzzy state matrix is judged to reach a preset association threshold value through a fuzzy rule base; when the abnormal state flag bit indicates that the isolated metabolism is abnormal, a second-level control instruction is generated; the driving unit is used for responding to the first-stage control instruction and cooperatively driving the buffer solution injection unit and the nutrition supplementing pump; responding to the secondary control instruction, and dynamically adjusting the opening degrees of a stirring rate controller, a temperature feedback loop and a gas proportional valve of the bioreactor. The control efficiency and the cell culture stability are improved.

The invention relates to the technical field of biological medicine, in particular to a hepatitis A inactivated vaccine and a preparation method thereof. Comprising the following steps: cell amplification, virus culture, virus harvesting, cell disruption, ultrafiltration concentration, inactivation, purification and finished product obtaining. The virus culture comprises the following steps: separating from excrement of a hepatitis A patient and carrying out adaptive passage to obtain a virus strain; adding a carrier into a bioreactor, sterilizing for later use, inoculating a virus strain into a collected cell suspension, and transferring into the bioreactor for culturing; the cell disruption step comprises the following steps: carrying out ultrasonic oscillation on a collected virus harvesting liquid to obtain a cell disruption liquid; trichloromethane is added for extraction and centrifugation, and supernatant virus liquid is collected; the invention provides a preparation method of a hepatitis A inactivated vaccine, which is used for preparing the efficient, safe and stable hepatitis A inactivated vaccine by optimizing virus culture and inactivation processes. The method has the advantages of high virus yield and low production cost, and is suitable for large-scale production and application.

The invention relates to a cell culture system (1) comprising: A bioreactor (100), comprising a vessel (110) and a recycling outlet (120), said recycling outlet (120) being positioned in the vessel (110) so as to be able to extract, from the vessel (110), a multiphase flow comprising a liquid phase and a gas phase; a separator (300) receiving the multiphase flow, said separator (300) being configured to accommodate and maintain a multiphasic system, comprising both the liquid phase and the gas phase; and a recycled media transfer line (220) adapted for routing of at least a portion of the liquid phase from the separator (300) to the bioreactor (100). The invention also relates to a cell culture method (1000) using a cell culture system (1) of the invention and cell biomass obtained thereof.

The invention relates to a method for producing notoginsenoside and ginsenoside by utilizing panax notoginseng cotyledon embryos, and belongs to the technical field of plant adventitious root tissue culture. The method comprises the following steps: inducing embryogenic calluses by utilizing immature zygotic embryos of panax notoginseng; proliferating the panax notoginseng embryogenic callus on a plate culture medium; s2, putting the pseudo-ginseng embryogenic callus obtained in the step S2 into a liquid culture medium, carrying out cell dispersion, and sieving by using a sieve with the aperture of 24-30 meshes; a mechanical stirring type bioreactor is filled with a liquid culture medium, and the globular embryo and the heart-shaped embryo are placed in the mechanical stirring type bioreactor to be cultured; s3, recovering the filtrate in the step S3, filtering by using a screen with the aperture of 30-80 meshes and 80-150 meshes to obtain uniform panax notoginseng embryogenic cells, and differentiating the panax notoginseng embryogenic cells in a triangular flask for the step S4; and drying the cotyledon embryo obtained in the step S4 at 45 DEG C, and grinding into powder. According to the method, large-scale generation of cotyledon embryos can be completed only in 4-6 weeks, the production process is remarkably accelerated, the method is suitable for industrial batch production requirements, and the production efficiency is high.

A fluid filtration system includes a fluid storage vessel such as a bioreactor, a filter housing including a filter element disposed therein, a pump coupled between the fluid storage vessel and the filter housing, and a flow diverter disposed between the pump and the filter housing. The pump is configured to move fluid from the fluid storage vessel through the filter element, and the flow diverter is configured for selectively directing fluid received from the pump to the first or second end of the filter housing. In a first mode of operation, the flow diverter directs flow through the filter housing in a first direction, while in a second mode of operation, the flow diverter directs flow through the filter housing in a second direction opposite the first direction.

The invention discloses a method for constructing a multicellular living body material by adopting a droplet bioreactor, which belongs to the crossing field of biological materials and microbial engineering, and comprises the following steps: (1) resuspending different cells in different polymer solutions to prepare degradable or phase-separable hydrogel particles containing cells; (2) dispersing the hydrogel particles in a cross-linkable hydrogel matrix to form a composite hydrogel; and (3) curing the composite hydrogel, and then carrying out degradation treatment to obtain the liquid cavity structure multicellular system bioreactor with spatial partition and dynamic interaction characteristics. According to the invention, ordered distribution and metabolism synergy of different microorganisms in space can be realized, controllable degradability and excellent mass transfer performance are realized, bacterial slow release and metabolite exchange can be maintained, and the method is suitable for long-term stable operation of a continuous flow reactor. The method has wide application prospects in the fields of green biological manufacturing, renewable energy source production, environmental restoration, biomedical engineering and the like.

The invention relates to the technical field of shellfish breeding, in particular to a rapid microalgae breeding method for shellfish breeding based on a photobioreactor and a cement pool. The method comprises the following steps: firstly, optimizing a photobioreactor structure and an illumination layout, controlling a culture environment by matching with a multi-parameter regulation and control system, breeding microalgae seeds with high resistance and high carbon sequestration capacity, culturing to a preset density, and partially harvesting and inoculating into a cement pond, after partial harvest, the photobioreactor realizes continuous culture by supplementing disinfected seawater and nutritive salt; the harvested high-density microalgae are inoculated into a cement pond, nutritive salt is supplemented in a doubled mode, air and carbon dioxide mixed gas is used for continuous aerated culture, and the microalgae are promoted to rapidly reach the index growth period and used for being directly fed to shellfish larvae; according to the rapid microalgae breeding method for shellfish seedling culture, the yield and quality of microalgae are both considered, the success rate and productivity of aquatic seedling culture are effectively improved, the production cost and the labor intensity of workers are reduced, and the method is suitable for large-scale popularization.

The invention discloses a water treatment device of an MABR membrane aeration bioreactor and a treatment method of the water treatment device, and relates to the field of sewage treatment.The water treatment device comprises a vertical frame body, oxygen permeation membranes are evenly arranged in the vertical frame body, and first communicating pipes are arranged at the front ends and the rear ends of the oxygen permeation membranes; the surface of the first communicating pipe at the rear end fixedly communicates with a first conveying pipe, the first communicating pipe is used for conveying oxygen to the oxygen permeation membrane, the first communicating pipe is connected with the vertical frame body through a movement assembly, and through double-form switching of the movement assembly, the oxygen permeation membrane is driven to conduct cyclic twisting and swinging, so that the oxygen permeation membrane is driven to conduct oxygen permeation. The contact of oxygen between the surface of the oxygen-permeable membrane and the microbial biological membrane is enhanced, the oxygen utilization rate is increased, the motion state of the membrane is dynamically adjusted, the self-cleaning capacity of the membrane is enhanced, and sludge deposited on the outer surface of the oxygen-permeable membrane is digested while the acceleration mechanism continuously swings at the bottom of the oxygen-permeable membrane to accelerate the contact of the oxygen-permeable membrane and pollutants in water.

1. The name of the product of this design: bioreactor. 2. Purpose of the product of this design: bioreactor for culturing cells. 3. The design key point of this appearance design product lies in the shape. 4. The picture or photo that best illustrates the design points: three-dimensional picture.

The invention discloses a method for realizing municipal sewage denitrification by combining pre-oxidation with a nano zero-valent iron loaded activated carbon carrier, and aims to solve the problems of slow growth speed of iron ammonia oxidation microorganisms, poor denitrification effect and the like in an iron ammonia oxidation process. The water treatment method comprises the following steps: 1, adding ferrate into municipal sewage to carry out pre-oxidation treatment, and adding polymeric ferric sulfide to carry out enhanced coagulation treatment; 2, inoculating mixed sludge into the SBR bioreactor, wherein the mixed sludge is composed of anaerobic ammonia oxidation granular sludge and municipal sludge; 3, introducing inert gas into the SBR reactor; and 4, introducing the pre-oxidized and coagulated sewage supernatant as inlet water into an SBR (Sequencing Batch Reactor) inoculated with sludge, and regularly adding carbon-loaded nano zero-valent iron into the SBR to complete denitrification of the municipal sewage. According to the invention, ferrate is added into municipal sewage for pre-oxidation through the front end, so that partial removal of organic matters and phosphorus is realized, and nitrogen removal of sewage is realized in combination with an activated carbon carrier loaded with nano zero-valent iron at the rear end.

The present disclosure relates to a biodegradable multilayer film comprising stacked on each other in the following order: a) a first outer layer comprising a first polymer selected from the group consisting of poly (butylene adipate-co-terephthalate) (PBAT), poly (butylene succinate) (PBS), poly (butylene succinate-co-adipate) (PBSA), and mixtures thereof; b) a core layer comprising a blend of thermoplastic starch (TPS) and polybutylene adipate terephthalate (PBAT); c) a second outer layer comprising a second polymer selected from the group consisting of poly (butylene adipate-co-terephthalate) (PBAT), poly (butylene succinate) (PBS), poly (butylene succinate-co-adipate) (PBSA), and mixtures thereof. The present disclosure also relates to a disposable bag, a bioreactor and a container suitable for mixing, packaging, storing or transporting biopharmaceutical substances, wherein the disposable bag, bioreactor and container comprise the biodegradable multilayer film.

System and method for providing biocompatible, nutrient filled media to the Human Cells, Tissues, and cellular and tissue-based Products (HCT / P) while removing wastes. The present teachings provide for sensing the characteristics of the media, and modifying the characteristics when necessary. The present teachings can also provide components that can provide fluid pumping integrated with fluid gas exchange, and sensing of fluid characteristics at consistent times during the fluid flow cycle. System and method control multiple bioreactors from a centralized media reservoir, while fluidically isolating the bioreactors from cross-contamination.

A bioreactor exhaust system includes an exhaust tube, a shut off valve, and a vessel. The exhaust tube includes a level sensor. The vessel includes a sterilant. The shut off valve is responsive to the sterilant reaching the level sensor.

The invention discloses a Yarrowia lipolytica strain for synthesizing ergothioneine and application of the Yarrowia lipolytica strain, and belongs to the technical field of genetic engineering and bioengineering. According to the invention, a key enzyme TrEGT1 is subjected to semi-rational transformation, so that the ergothioneine yield of a strain expressing a mutant is increased by 240.9%, precursor metabolic network screening is further modularized, precursor flux is enhanced, a competitive approach of a precursor is knocked out and weakened, and strategies such as fermentation process optimization and the like are further optimized; and when the constructed strain is fermented in a 5L bioreactor, the yield of ergothioneine can reach 10.3 g / L. The highest-level ergothioneine synthesis reported at present is realized, the feasibility of synthesizing the ergothioneine by using the Yarrowia lipolytica as the chassis cell is verified, and a reference value is provided for efficiently synthesizing the ergothioneine by using other chassis cells.

The present disclosure relates to processes for producing hydrocarbon fuels from lignocellulosic biomass. A process may include introducing biomass to a pretreatment system, and a first separation system forming a pentose-rich stream and a pentose-lean stream. The pentose-lean stream may be introduced to a hydrolysis system forming a hydrolysate and the hydrolysate introduced to a second separation system forming a hexose-rich stream and a lignin stream. Additionally, at least one of the pentose-rich stream or the hexose-rich stream may be introduced to a bioreactor containing microorganisms configured to produce hydrocarbon fuels. Additionally, the present disclosure also relates to systems for the production of hydrocarbon fuels. A system may include a pretreatment system, a first separation system, a hydrolysis system, a second separation system, and one or more bioreactors. Alternatively a system may include a pretreatment system, a hydrolysis system, a sugar separation system, and one or more bioreactors.

The invention discloses a recombinant subunit vaccine for resisting trichomonas pigeonae infection and a preparation method of the recombinant subunit vaccine. The vaccine comprises two recombinant proteins with sequences as shown in SEQ ID NO: 7 and SEQ ID NO: 8 respectively and a pharmaceutically acceptable carrier. According to the invention, Sf9 cells are used for respectively expressing trichomonas pigeonae adhesion protein 33 and adhesion protein 65, the immunogenicity of the obtained recombinant protein is similar to that of natural protein, the expression level is high, the immunogenicity is strong, a very small amount of recombinant protein can provide a better immune protection effect, and the recombinant protein has no pathogenicity to pigeons, and can be used for preparing the recombinant protein for preventing and treating trichomonas pigeonae. Meanwhile, large-scale serum-free suspension culture can be carried out through a bioreactor, so that the vaccine production cost is greatly reduced.

Systems and methods for producing ammonium nitrate are disclosed herein. Exemplary systems can comprise a bioreactor positioned to receive a feed including ammonia. The bioreactor can hold a liquid solution including (i) an ammonia oxidizing bacteria (AOB) and (ii) a nitrite oxidizing bacteria (NOB). The AOB can facilitate oxidation of the ammonia to produce a nitrite at a nitritation rate and the NOB can facilitate oxidation of the nitrite at a nitratation rate. Thus, the bioreactor can produce a mixed liquor comprising a nitrate biologically. The system can further comprise a filter positioned to receive the mixed liquor and produce a permeate and a sludge. A sensor can measure the concentration of the liquid solution of the bioreactor, and a controller coupled to the sensor can regulate the mass flow of nitrogen and / or phosphorus of the bioreactor feed based on the measured concentration of the liquid solution.

Photobioreactors are described herein. The photobioreactors include a vessel for cultivating microalgae. The vessel defines an interior chamber comprising a culture containing medium. The vessel has at least one transparent wall to permit passage of light of a frequency necessary to promote growth of the microalgae in the culture containing medium. The vessel also includes a plurality of sensors, each sensor being configured to monitor at least one environmental condition within the photobioreactor, and at least one flow generator positioned within the vessel. The photobioreactor also includes a controller communicatively coupled to each of the plurality of sensors and the at least one flow generator. The controller is configured to: receive sensor data from each of the plurality of sensors, apply a mathematical model to the sensor data to determine when to adjust at least one environmental condition within the photobioreactor and adjust one or more components of the photobioreactor.

The current disclosure concerns a system for producing biomolecules comprising a docking station (30), said docking station encompasses: —a bioreactor (1); —a concentrator (2) fluidly linked to the bioreactor; —an intermediate vessel (4), positioned between said bioreactor (1) and concentrator (2), wherein said intermediate vessel (4) and concentrator (2) are connected by a retentate conduit (303), allowing recirculating of liquid from an output of the concentrator to an input of said intermediate vessel and—a controller, integrated in said docking station (30), which is able to control the biomolecule process.

The invention relates to a bioreactor (100) adapted for culturing animal cells, comprising: a vessel (110) defining a cultivation volume (111); at least two gas exchange devices (120) protruding in the vessel (110), each gas exchange devices (120) having a gaseous exchange surface (121) in the vessel (110); and at least one media inlet (130) and at least one media outlet (135), said media inlet (130) and outlet (135) being configured to operate simultaneously. The invention also relates to methods, cell biomass obtained from these methods and edible food products including this biomass.

The invention discloses an immobilized enzyme reactor for preparing low-carbon-chain triglyceride, and relates to the technical field of bioreactors, the immobilized enzyme reactor comprises a reactor body, a bearing ball carrying enzyme, a feeding pipe, a discharging pipe, a conveying channel, a conveying mechanism and a collector; a filter pipe is arranged in the reactor body, the upper end of the filter pipe is communicated with the conveying channel through a feeding pipe, the lower end of the filter pipe is communicated with a collector through a discharging pipe, and the lower end of the collector is communicated with the conveying channel; unused bearing balls are contained in the collector, when the immobilized enzyme needs to be recycled, the vibrator on the filter pipe vibrates the filter pipe, the bearing balls in the filter pipe roll into the collector from the discharging pipe under the combined action of vibration and gravity, and the unused bearing balls in the collector are moved to the feeding pipe through the conveying mechanism and finally enter the filter pipe; an operator can open the cover plate of the collector and take out the used bearing balls from the discharge port, so that the effect of recycling the immobilized enzyme is realized.

The invention relates to recombinant pichia pastoris for high expression of porcine pancreatic phospholipase A2 and application of the recombinant pichia pastoris, and belongs to the technical field of genetic engineering. According to the method disclosed by the invention, a vacuole sorting receptor Vps10 is knocked out by utilizing a CRISPR-Cas9 system, a CPY sorting way between a trans-golgi apparatus and a vacuole is interrupted, and ppPLA2 degradation is reduced; then, in order to relieve unfolded protein response in the endoplasmic reticulum, molecular chaperones are introduced into the genome, and synthesis of ppPLA2 is enhanced; the expression intensity of the ppPLA2 is improved by increasing the gene copy number of the ppPLA2; and finally, high-density fermentation is performed in a 3-L bioreactor, so that high-efficiency expression of the ppPLA2 in the pichia pastoris is realized.

This disclosure relates to large-scale bioreactor systems comprising a vessel comprising internal reaction chamber having a volumetric and cell-sustaining capacity significantly above that of currently available bioreactor systems.

A method and apparatus for inoculating a bioreactor with a C1-fixing strain is disclosed. In particular, the present disclosure relates to the cost-efficient methods and apparatus for inoculating a bioreactor with a C1-fixing strain for production of protein, such as single cell protein or cultured protein, by microbial fermentation of a gaseous substrate.