236 results about "Perfusion fluid" patented technology

The present invention provides a method of extracting and recovering embryonic-like stem cells, including, but not limited to pluripotent or multipotent stem cells, from an exsanguinated human placenta. A placenta is treated to remove residual umbilical cord blood by perfusing an exsanguinated placenta, preferably with an anticoagulant solution, to flush out residual cells. The residual cells and perfusion liquid from the exsanguinated placenta are collected, and the embryonic-like stem cells are separated from the residual cells and perfusion liquid. The invention also provides a method of utilizing the isolated and perfused placenta as a bioreactor in which to propagate endogenous cells, including, but not limited to, embryonic-like stem cells. The invention also provides methods for propagation of exogenous cells in a placental bioreactor and collecting the propagated exogenous cells and bioactive molecules therefrom.

A delivery system to provide end organ oxygenation and even systematic oxygenation in the face of ischemic result. The deliver system including a retrograde oxygenation and perfusion stent. The stent employing at least two and possibly more channels to allow flow of the perfusate from the device to the renal pelvis then to a back out to a collection apparatus. The stent may include various vital sign monitors, such as a renal pressure monitor, temperature monitor, and even an oxygenation monitor. The stent may include an anchoring device to allow the stent to be anchored into the renal pelvis in a temporary way during the retrograde oxygenation process.

Methods and systems of maintaining, evaluating, and providing therapy to a lung ex vivo. The methods and systems involve positioning the lung in an ex vivo perfusion circuit; circulating a perfusion fluid through the lung, the fluid entering the lung through a pulmonary artery interface and leaving the lung through a left atrial interface; and ventilating the lung by flowing a ventilation gas through a tracheal interface. Maintaining the lung for extended periods involves causing the lung to rebreath a captive volume of air, and reaching an equilibrium state between the perfusion fluid and the ventilation gas. Evaluating the gas exchange capability of the lung involves deoxygenating the perfusion fluid and measuring a time taken to reoxygenate the perfusion fluid by ventilating the lung with an oxygenation gas.

An organ preservation system having an organ chamber with a perfusate reservoir surrounded by a heat exchanger. The system has a pump for circulating perfusate, which draws perfusate from the reservoir and passes the perfusate through a bubble trap, and temperature and pressure sensors prior to entering the chamber where the organ is perfused. An oxygenator with a platform and a filter is situated within the perfusate reservoir such that perfusate leaving the organ which rests on the platform passes through the filter before dripping into the perfusate reservoir below. The pump re-circulates perfusate from the perfusate reservoir. The oxygenator has gas permeable tubes wound around support legs of the oxygenator. Circulation of oxygen through the tubes allows for gas exchange by diffusion across the membrane to oxygenate perfusate flowing around the tubes.

Electrode systems have been developed for use in perfusion systems to measure the electrical activity of an explanted heart and to provide defibrillation energy as necessary. The perfusion systems maintain the heart in a beating state at, or near, normal physiological conditions; circulating oxygenated, nutrient enriched perfusion fluid to the heart at or near physiological temperature, pressure and flow rate. These systems include a pair of electrodes that are placed epicardially on the right atrium and left ventricle of the explanted heart, as well as an electrode placed in the aortic blood path.

Microfluidic devices and methods for perfusing a cell with perfusion fluid are provided herein, wherein the gravitational forces acting on the cell to keep the cell at or near a retainer or a retaining position exceed the hydrodynamic forces acting on the cell to move it toward an outlet.

Methods and systems of maintaining, evaluating, and providing therapy to a lung ex vivo. The methods and systems involve positioning the lung in an ex vivo perfusion circuit; circulating a perfusion fluid through the lung, the fluid entering the lung through a pulmonary artery interface and leaving the lung through a left atrial interface; and ventilating the lung by flowing a ventilation gas through a tracheal interface. Maintaining the lung for extended periods involves causing the lung to rebreath a captive volume of air, and reaching an equilibrium state between the perfusion fluid and the ventilation gas. Evaluating the gas exchange capability of the lung involves deoxygenating the perfusion fluid and measuring a time taken to reoxygenate the perfusion fluid by ventilating the lung with an oxygenation gas.

Methods and systems of maintaining, evaluating, and providing therapy to a lung ex vivo. The methods and systems involve positioning the lung in an ex vivo perfusion circuit; circulating a perfusion fluid through the lung, the fluid entering the lung through a pulmonary artery interface and leaving the lung through a left atrial interface; and ventilating the lung by flowing a ventilation gas through a tracheal interface. Maintaining the lung for extended periods involves causing the lung to rebreath a captive volume of air, and reaching an equilibrium state between the perfusion fluid and the ventilation gas. Evaluating the gas exchange capability of the lung involves deoxygenating the perfusion fluid and measuring a time taken to reoxygenate the perfusion fluid by ventilating the lung with an oxygenation gas.

Microfluidic devices and methods for perfusing a cell with perfusion fluid are provided herein, wherein the gravitational forces acting on the cell to keep the cell at or near a retainer or a retaining position exceed the hydrodynamic forces acting on the cell to move it toward an outlet. Also provided, are methods for assaying cell products within the microfluidic device.

An isolated heart or heart-lung preparation in which essentially normal pumping activity of all four chambers of the heart is preserved, allowing for the use of the preparation in conjunction with investigations of electrode leads, catheters, ablation methods, cardiac implants and other medical devices intended to be used in or on a beating heart. The system can be designed to be used within a Magnetic Resonance Imaging (MRI) unit or a X-ray computed tomography (CT) scanner. The preparation may also be employed to investigate heart and lung functions, in the presence or absence of such medical devices. In order to allow comparative imaging visualizations of either or simultaneously the heart and/or lung structures and devices located within the chambers of the heart or vessels or bronchi within the lungs, a clear perfusate such as a modified Krebs buffer solution with oxygenation is circulated through all four chambers of the heart and thus the coronary and/or pulmonary vasculatures. A ventilator with intubation tube can be used to inflate/deflate the lungs and/or provide oxygen to the isolated organs. The preparation and recordings of the preparation may be used in conjunction with the design, development and evaluation of devices for use in or on the heart and/or lungs, as well as for use as an investigational and teaching aid to assist physicians and students in understanding the operation of the cardiopulmonary system.

The present invention provides a system for chemohyperthermia treatment. The chemohyperthermia treatment system comprises a reservoir for storing fluid; a heating/cooling system coupled to the reservoir so that the fluid can be transferred from the reservoir to the heating system, wherein the heating/cooling system comprises a heating/cooling exchange module having a channel within which the fluid can flow; and a plurality of peltier modules coupled to the heating/cooling module, wherein the plurality of peltier modules heat up the fluid flowing through the channel, and wherein in the cooling mode, the plurality of peltier modules cool the fluid flowing through the channel; a pumping means coupled to the heating/cooling system, wherein the pumping means pump the perfusion fluid from the reservoir to the heating/cooling system, thereby allowing the heating/cooling system to change the temperature of the fluid; at least one inflow catheter coupled to the pumping means, wherein the at least one inflow catheter delivers the heated/cooled fluid to an object; and at least one outflow catheter coupled to the reservoir, wherein the at least one outflow catheter drains the fluid from the object to the reservoir.

The invention relates to a multifunctional perfusion and suction platform and a using method. The perfusion and suction platform at least comprises a perfusion device and a main control unit and further comprises a pipe pressure sensor for measuring pressure in a perfusion fluid channel of the perfusion device, a cavity pressure sensor for measuring pressure of an inner cavity and an alarming device; the pipe pressure sensor, the cavity pressure sensor and the alarming device are all connected with the main control unit, limit reference values of the pipe pressure sensor and the cavity pressure sensor are preset in the main control unit, and on the condition that the pressure measured by the pipe pressure sensor or the pressure measured by the cavity pressure sensor exceeds the corresponding limit reference value, the alarming device is started. The structure has the advantages that the pipe pressure and the cavity pressure are monitored simultaneously, double insurance is formed, a doctor can know a failure through the alarming device and make a right judgment at first time according to the pressure measured by the other sensor even if one of the sensors fails, and therefore the operation safety is greatly improved.

The invention relates to an isolated organ preserving device and a preserving method thereof, and belongs to the technical field of medical equipment. The isolated organ preserving device is structurally characterized in that continuous mechanical irrigation and membrane oxygenation are adopted, an isolated organ continuously obtains nutrients and the oxygen supply, a cell metabolite is removed in time, and a cell microenvironment is better maintained, so that the function normality is ensured. The isolated organ preserving device disclosed by the invention can be used for directly contacting the organ perfusate and the organ perfusion instrument by adopting a disposable consumable material sterilized through epoxyethane, thereby meeting the requirement for sterility of a system. Besides, the isolated organ preserving device disclosed by the invention can be used for judging the consumption of the nutrients contained in the organ perfusate and the gathering degree of metabolic wastes by monitoring the pH value of the organ perfusate in real time and can be used for abandoning the organ perfusate after a certain standard is achieved and replacing the fresh organ perfusate, thereby further ensuring the possibility of long-time preservation; the isolated organ preserving device can be used for evaluating the damage condition of the isolated organ by monitoring the front and back perfusion pressure of the isolated organ in real time and converting the perfusion resistance by combining a flow number.

The invention relates to a kidney storage device and provides a sub-normal-temperature or low-temperature isolated kidney storage device comprising an organ storage box, a liquid storage chamber, a perfusing pipeline and a controller, wherein the liquid storage chamber is connected with an input opening of the perfusing pipeline through a one-way valve; an output opening of the perfusing pipeline is connected with the organ storage box through a one-way valve; the organ storage box is connected with the input opening of the perfusing pipeline through a one-way valve; the organ storage box is also connected with a urine and perfusate collecting and sampling window; a detecting device connected with the controller is arranged between the perfusing pipeline and the organ storage box; and the organ storage box is provided with a temperature control device. The sub-normal-temperature or low-temperature isolated kidney storage device is used for storing kidneys.

The invention relates to a vascular tissue engineering reactor which is characterized by comprising intravascular perfusion liquid storage bottles (101) which are connected with each other in sequence by pipelines, a pulsation source (102) which is taken as an intravascular perfusion liquid driving device, a culture cavity inlet pipeline (202) of an intravascular perfusion circuit, and a vascular tissue culture cavity (107), wherein, one end of an inlet pipeline (104) of the culture cavity enters the vascular tissue culture cavity (107), and is connected with one end of a vascular tissue (108) to be cultured so as to cause the culture solution from the perfusion liquid storage bottle (101) to flow into the vascular tissue (108). The reactor further comprises a culture cavity outlet pipeline (204) of the intravascular perfusion circuit, one end of the culture cavity outlet pipeline (204) enters the vascular tissue culture cavity (107), and is connected with the other end of the vascular tissue (108) to be cultured so as to export the culture solution from the inner part of the vascular tissue (108).

The invention discloses a kit for a pancreas decellularized scaffold and preparation and reseeding methods of the scaffold. The kit comprises perfusion fluid 1, perfusion fluid 2 and perfusion fluid 3, wherein the perfusion fluid 1 comprises 0.25 g/L of EDTA (ethylene diamine tetraacetic acid), 10,000 U/L of heparin sodium and PBS (phosphate buffer solution); the perfusion fluid 2 comprises 1% Triton-X100 and 0.1% ammonia water; the perfusion fluid 3 comprises 0.01 g/L of DNase I (eoxyribonuclease I). The kit has the characteristics that the expense is low, cells are radically removed, a detergent is easily eliminated, the structures of extracellular matrixes and blood vascular systems are well preserved, the reseeding and growth of exogenous and endogenous cells are facilitated, and the exogenous and endogenous cells can be conveniently transplanted to diabetic experiment animals.

The invention relates to an isolated organ perfusion system and a liver storage device. The liver storage device comprises a container body and a breathing air bag. The container body is used for containing perfusate. The breathing air bag is arranged in the container body and used for supporting an isolated organ. According to the liver storage device, the breathing air bag is additionally arranged in the liver storage device, the isolated organ is placed on the breathing air bag, breathing and inhaling of the isolated organ are simulated during perfusion, and the breathing air bag is subjected to air suction and inhaling, so the breathing air bag is continuously relaxed in the perfusion process, and the point of the contact surface of the isolated organ and the breathing air bag is correspondingly changed; and thus, blood circulation of the isolated organ can be smoother in the perfusion process, thrombus generated on the surface of the isolated organ due to long-time fixed pressureis avoided, an invalid perfusion area can be effectively eliminated, and continuous local compression necrosis is reduced.