40 results about "Perfusion fluid" patented technology

An intravascular device and methods of using the same are provided. An expandable portion includes struts movable between a collapsed position and an expanded position, and tissue modification element(s), each having a lower portion residing within or attached to one of the struts, an upper portion which extends outwardly from an outer surface of the strut and physically interacts with tissue during use. Irrigation and / or suction device(s) are provided for providing at least one of irrigation fluid and suction force at the expandable portion when operated.

This invention relates to a method and application for enriching circulating tumor cells (CTCs) based on ambient temperature mechanical perfusion technology, belonging to the field of biomedical technology. The method comprises the following steps: flushing the liver resected during liver transplantation via the portal vein and hepatic artery, connecting it to an ambient temperature mechanical perfusion loop, and performing in vitro perfusion using simulated blood perfusion fluid; when perfusion exceeds 2 hours and the lactate content drops to <5 mmol / L, collecting the simulated blood perfusion fluid; and sequentially subjecting the simulated blood perfusion fluid to centrifugation, red blood cell removal, and white blood cell removal to obtain enriched circulating tumor cells (CTCs) for liver cancer. A human liver cancer ex vivo organ perfusion model is constructed using NMP technology to fully recreate the tumor microenvironment and CTC generation process, and the large volume of perfusion fluid is processed to achieve efficient enrichment of CTCs.

The subject matter of the present invention is a perfusion fluid in the form of an oil-in-water PFC nanoemulsion, methods of producing this perfusion fluid, and its use in particular for organ perfusion and as a blood substitute preparation.

The present application relates to a kind of arthroscopic approach water leakage prevention device, including the elastic cover with dome structure, the sealing valve being arranged in the center position of cover and negative pressure suction mechanism, sealing valve has the conical hollow structure extending to base direction and gradually reducing outer diameter, reverse compensation sealing is realized using joint cavity infusion liquid pressure, cover base edge is equipped with flexible sealing skirt, by the negative pressure interface connection external negative pressure source, so that cover and skin form negative pressure sealing space, the device can be connected in series by negative pressure interface multiple occluder, and equipped with detachable guide rod auxiliary coaxial positioning.The present application uses non-insertion type surface adsorption occlusion, effectively solve the space interference and tissue damage problem in multiple tunnel operation, realize the dynamic water stop of pressure self-adaption, with the advantages of simple operation, sealing stable, strong dissection adaptability, can significantly improve the operation visual clarity and maintain joint cavity pressure stability.

The application discloses an ultrasonic emulsification perfusion assembly, which comprises a metal connector, a plastic needle tube arranged in the metal connector, a metal perfusion sleeve provided with a water inlet and a perfusion port, and a plastic clamping perfusion sleeve which is sleeved outside the metal perfusion sleeve and forms a liquid storage space, wherein the water inlet is arranged in the liquid storage space. The application has the following beneficial effects: the slender needle head of the plastic needle tube can improve the stability of the anterior chamber and reduce the probability of surge occurrence; the relatively soft plastic needle head tip can also reduce the probability of puncturing the lens capsule; the metal perfusion sleeve effectively separates the ultrasonic emulsification needle tube body from the extrusion of the incision, greatly reduces or even eliminates the incision burn caused by ultrasonic friction; the relatively hard metal perfusion sleeve effectively prevents the perfusion liquid from being reduced or blocked due to the extrusion or distortion of the incision, thereby improving the stability of the anterior chamber and reducing the probability of surge occurrence.

The present disclosure describes techniques for providing directed instillation or irrigation of fluid during wound therapy to deliver cleansing fluid precisely onto a wound to improve wound cleansing. Directed instillation or irrigation of fluid onto wounds using techniques of the present disclosure can accelerate wound healing and provide improved patient outcomes. One example of the present disclosure is a device for providing directed fluid irrigation to a wound. The device can include an irrigation tube extending between a first end and a second end, with the second end having a perforated section or a non-perforated closed section. The device can further include a foam structure arranged around the perforated section, with the foam structure being configured to dispense fluid received from the perforated section uniformly into a wound for cleansing. The perforated section can include a plurality of perforations or a plurality of perforated irrigation tubes in examples.

A parathyroid gland perfusion preservation device is provided, which relates to the field of human organ preservation technologies. The device includes a parathyroid gland preservation box, a perfusion liquid circulation system and a control system. The parathyroid gland preservation box defines a space for placing parathyroid glands. The perfusion liquid circulation system is connected to the parathyroid glands stored in the parathyroid gland preservation box through inlet pipes, indwelling needles and a reflux pipe to supply perfusion liquid to the parathyroid glands. The control system is electrically connected to components of the perfusion liquid circulation system, and configured to receive a working state signal of the components of the perfusion liquid circulation system, and send a control instruction to the components of the perfusion liquid circulation system according to the working state signal. The device can achieve in vitro preservation of parathyroid glands, and can effectively protect the parathyroid glands.

This invention belongs to the field of biomedical technology and provides a high-throughput drug screening system and its usage method for enriching circulating tumor cells (CTCs) in liver cancer. It includes an information processing and control unit; a diseased liver perfusion device comprising an organ chamber containing injection fluid at the bottom, an arterial supply line, an arterial end sensor, an arterial end clamp valve, at least two membrane lungs, a portal vein supply line, a portal vein end clamp valve, and a portal vein end sensor; a CTC enrichment device, which uses erythrocyte lysis to remove erythrocytes and immunomagnetic bead labeling to remove leukocytes, enabling the processing of large volumes of perfusion fluid in a single step to obtain enriched circulating tumor cells; a high-throughput drug screen; and phototubes, arterial end sensors, and portal vein end sensors, each electrically connected to the information processing and control unit. Compared with existing technologies, the high-throughput drug screening system and its usage method of this invention have the advantages of establishing a room-temperature mechanically perfused ex vivo liver cancer model, realizing the processing of large volumes of liquid specimens, and providing automated high-throughput drug screening functionality.

This utility model belongs to the field of living organism preservation technology, and in particular relates to a limb perfusion and transport device. The limb perfusion and transport device includes an insulated outer shell, a limb box, a solution container, and a perfusion system. The insulated outer shell contains a cold zone chamber and a waste liquid chamber for containing a waste liquid bag; the limb box contains a limb chamber for containing the severed limb, and is at least partially located within the cold zone chamber; the solution container contains a solution chamber for containing the perfusion fluid, located within the cold zone chamber, and is spaced apart from the limb box in the X direction. A filling port for adding a cold-storing substance to the cold zone chamber is located between the solution container and the limb box; the perfusion system is located within the insulated outer shell and connects the solution container, the limb box, and the waste liquid bag, so that the perfusion fluid in the solution chamber is infused into the severed limb in the limb chamber and then into the waste liquid bag. In this device, the perfusion fluid in the solution container is only infused once, ensuring the viability of the severed limb, and the miniaturized design facilitates transport.

A system for ex vivo normothermic organ preservation may include a pump configured to circulate a perfusate through a perfusion circuit, an oxygenator coupled configured to oxygenate the perfusate, a heat exchanger configured to regulate a temperature of the perfusate and an organ chamber having an internal volume configured to receive an organ. The perfusion circuit may be configured to be coupled in fluid communication with an arterial inlet into the organ. The system may also include a processor and memory having instructions that when executed by the processor cause the system to carry out a method. The method may include changing a temperature of the perfusate from an initial temperature to a normothermic temperature within a normothermic temperature range, maintaining the perfusate within the normothermic temperature range for at least one hour, receiving sensor data for perfusate parameters, and displaying the sensor data.

The utility model discloses a kind of automatic continuous filling's corpse antiseptic high-pressure perfusion device, including liquid storage tank, medicament box, automatic liquid preparation liquid filling device, medicament box is sequentially connected liquid storage tank by medicament branch pipe, first solenoid valve, first flowmeter, medicament main pipe, the upper portion of liquid storage tank is connected distilled water tank by water pipe, second flowmeter, second solenoid valve;Automatic liquid preparation liquid filling device includes liquid pump, controller and liquid level sensor, the import of liquid pump is connected liquid storage tank by liquid extraction pipe, the outlet of liquid pump is connected perfusion needle by gate valve, liquid guide pipe, hose;Liquid level sensor is arranged in liquid storage tank, and upper and lower limit switch of liquid level sensor is electrically connected with first solenoid valve, second solenoid valve, liquid pump switch and controller respectively.The utility model integrates automatic liquid preparation and high-pressure liquid filling function, can continuously, stably obtain sufficient perfusion fluid supply, realizes high pressure, automatic, continuous perfusion, improves perfusion efficiency and uniformity.

Systems and methods for preventing, treating, or managing the risk of infection in a closed subcutaneous cavity. In one example, the method comprises (a) implanting an elongated fluid conduit such that the fluid conduit communicates with a closed subcutaneous cavity, and (b) operating a perfusion system according to a perfusion treatment program, the operation of which comprises (i) delivering perfusion fluid from a perfusion fluid source through the fluid conduit to the closed subcutaneous cavity according to parameters of the perfusion treatment program, (ii) enabling the retention of the perfusion fluid in the closed subcutaneous cavity according to another parameter, and (iii) delivering the perfusion fluid from the closed subcutaneous cavity through the fluid conduit according to another parameter.

A perfusion apparatus (10) comprising a hermetically sealed casing (601) having an internal chamber (603) configured to hold a perfusate, the casing (601) comprising a perfusate inlet (614), a perfusate outlet (616), a gas inlet (632), and a gas outlet (620). A removable tray (612) is located within the internal chamber (603), the removable tray (612) comprising at least one drainage aperture (668), the removable tray (612) having a recess (661). An inflatable cushion (628) is located within the recess (661), wherein the cushion (628) is configured to support the perfusion target. A blood management module (22) is in fluid communication with the perfusate inlet (614) and the perfusate outlet (616), the blood management module (22) being configured to manage a circulating perfusate.

An endoscope includes a handle including a handle housing, an irrigation inlet port adapted to receive irrigation fluid, and an irrigation channel; and an insertion cord extending distally from the handle. The insertion cord includes an insertion tube; a bending section extending from the insertion tube; a distal tip extending from the bending section and including a tip housing; a camera at least partially enclosed in the tip housing; interstitial space within the insertion tube in fluid communication with the irrigation channel; and at least one interstitial flow opening in fluid communication with the interstitial space and adapted to discharge the irrigation fluid.

The present application relates to a kind of heart auxiliary system and perfusion device.Perfusion device includes driving element, transmission mechanism and at least two liquid injectors, liquid injector includes injection cavity and the piston rod in injection cavity, the end of injection cavity away from piston rod is equipped with injection port and suction port, injection port is used to communicate with heart auxiliary device, suction port is used to communicate with liquid container, piston rod is connected with transmission mechanism respectively, driving element is connected with transmission mechanism, for simultaneously driving at least one liquid injector in injection state and at least one liquid injector in suction state.Driving element starts to drive transmission mechanism movement, transmission mechanism simultaneously drives at least two liquid injectors to operate, so that at least one liquid injector injects liquid to heart auxiliary device while at least another liquid injector sucks liquid from liquid container, and alternately pushes liquid, ensure that there is always perfusion liquid injection in heart auxiliary device, realize continuous and stable perfusion, effectively reduce the blood damage in heart auxiliary device, reduce the risk of hemolysis.

The application provides an application of alpha-ketoglutaric acid in improving corneal endothelial injury and dysfunction, and belongs to the technical field of biological medicine. The application finds that, as a key metabolic intermediate in the tricarboxylic acid cycle, alpha-ketoglutaric acid can significantly reduce corneal endothelial cell injury and dysfunction induced by various causes such as high glucose, genetic defects, high intraocular pressure and surgical trauma by improving mitochondrial energy metabolism, reducing oxidative stress, stabilizing mitochondrial structure and membrane potential. Experiments prove that alpha-ketoglutaric acid can be used in the form of local administration such as eye drops, anterior chamber perfusion, systemic oral administration or combined administration, effectively reduces corneal edema, reduces central corneal thickness, shortens the recovery time of corneal transparency, and has a clear protection and repair effect on diabetic-related corneal endothelial lesions, Fuchs corneal endothelial dystrophy, glaucoma high intraocular pressure injury and intraocular surgery-related endothelial injury.

This invention provides an ophthalmic surgical apparatus and cassette capable of appropriately suppressing the effects of pulsation caused by a peristaltic pump. The ophthalmic surgical apparatus includes an infusion path, a peristaltic infusion pump, and an infusion fluid reservoir. The infusion path allows infusion fluid supplied from an infusion fluid source to pass through to surgical instruments. The peristaltic infusion pump is located within the infusion path and changes the pressure within the path by utilizing multiple pump rollers that rotate while pressing against the flexible infusion path. The infusion fluid reservoir is located in the path between the peristaltic infusion pump and the surgical instruments, storing the infusion fluid with an airtight interior.

This invention discloses a cystoscope with pressure detection function, belonging to the field of medical device technology. It comprises a sheath and a cystoscope body. The sheath is divided into a distal and proximal end, containing an irrigation chamber and a pressure measuring chamber. The pressure measuring chamber contains a sheath, the open end of which is sealed to the chamber wall, and the closed end extends proximally. The cystoscope body is adapted to the pressure measuring chamber and contains a sensor chamber, including a tension sensor and an adjustment component. The sensor is connected to the end of the adjustment component, and the closed end of the sheath is connected to the sensor via a first pull cable. The sensor measures tension data, and the adjustment component adjusts the axial position of the sensor, ensuring that the pull cable and sheath are taut after the cystoscope body is inserted. This invention uses a sheath within the pressure measuring chamber, dividing the internal space into an outer and inner side. Intrabladder pressure causes axial deformation of the sheath, which is converted into tension changes via the first pull cable and detected by the sensor. This is a mechanical transmission rather than a fluid-like transmission. Even if air bubbles enter the pressure measuring chamber from the irrigation fluid, the bladder pressure can still be effectively transmitted, and the sheath will deform accordingly, improving measurement accuracy.

The application relates to a perfusion liquid conveying device and a ventricular assist system. The perfusion liquid conveying device comprises a base, a rotating shaft, a bearing assembly and a buffer assembly, the base is internally provided with a perfusion channel, the bearing assembly comprises a plurality of bearings, the inner ring and the outer ring of the bearing have a flow gap therebetween, the buffer assembly is provided with a reflux cavity, the perfusion channel is an inflow channel of perfusion liquid, the perfusion liquid mixes with refluxed blood in the perfusion channel to form mixed liquid, the mixed liquid passes through the flow gap and the reflux cavity to be refluxed, part of the reflux cavity extends in the axial direction, and part of the reflux cavity extends in a first direction. The reflux cavity itself constitutes a zigzag loop, the reflux cavity can form a buffering effect on the mixed liquid, the flow of the mixed liquid in the perfusion liquid conveying device is inhibited, thereby the reflux of blood to the perfusion liquid conveying device is inhibited, the structural damage problem of the perfusion liquid conveying device caused by the reflux of blood is improved, and the working reliability of the perfusion liquid conveying device is improved.

The application provides a kind of for heart death after organ acquisition perfusion fluid and its application, belong to medical technical field.The application is used for heart death after organ acquisition perfusion fluid, including the following components: thrombolytic drug, vasodilator, albumin, dexamethasone sodium phosphate, low molecular weight heparin sodium and lactated Ringer's solution.The organ acquisition perfusion fluid of the application can effectively remove the thrombus formed by coagulation system activation, reduce vasospasm caused by warm ischemic injury and tissue cell edema in the process of perfusion, which can effectively improve the quality and function of the acquired organ, increase the utilization rate of the organ, and has clinical transformation application prospect.

Abstract The invention provides, in various embodiments, devices and methods relating to ex-vivo organ care. In certain embodiments, the invention relates to aortic cannulas for use in perfusion systems to return perfusate to the heart or delivering perfusate from the heart while the organ is sustained ex vivo at physiologic or near-physiologic conditions. Abstract 20 26 20 49 33 25 J un 2 02 6 A b s t r a c t 2 0 2 6 2 0 4 9 3 3 2 5 J u n 2 0 2 6

The application relates to the technical field of biomedical engineering, and discloses a method for constructing an ex vivo normal-temperature perfusion repair model based on a discarded liver. The method constructs a double-path closed-loop perfusion system, applies a shear stress flow field with low-frequency fluctuation characteristics to a portal vein loop to maintain endothelial function, loads a composite flow field obtained by superimposing low-frequency pulsation and high-frequency micro-shaking on a hepatic artery loop, and significantly reduces microcirculation flow resistance by using the thixotropy and shear thinning characteristics of oxygen-containing perfusion liquid. The system further realizes physical layering of red blood cells and lipids by using acoustic radiation force of a standing wave acoustic flow control layered cavity, and automatically guides a high-viscosity fat-rich flow layer into an adsorption module for specific purification by cooperating with a thixotropic passive shunt valve based on fluid viscosity feedback. Temperature-gas coupling adjustment based on a metabolic temperature coefficient is performed in a rewarming stage. The application solves the problems of microcirculation non-recurrent flow and hyperlipidemia clearance by coupling of multi-dimensional physical fields, and realizes effective repair of the discarded liver.

The invention relates to the technical field of medical instruments, and provides an organ perfusion control system and method.The system comprises a PLC control module, an actuator unit and a signal collecting unit, the actuator unit and the signal collecting unit are connected to the PLC control module, the actuator unit comprises a peristaltic pump and a temperature adjusting module, and the peristaltic pump is used for driving perfusate to be injected into an organ cabin; the temperature adjusting module is used for adjusting the temperature of the perfusate; the signal acquisition unit is used for acquiring perfusion parameters in the perfusion process, and the perfusion parameters comprise a temperature signal, a pressure signal and a flow signal of perfusate; the PLC control module dynamically adjusts the rotating speed of the peristaltic pump and the working state of the temperature adjusting module according to the received perfusion parameters, cooperative control over perfusion pressure, flow and temperature is achieved, the perfusion environment in the organ cabin can be kept stable during long-time operation, the perfusion state under the physiological condition is effectively simulated, and the perfusion efficiency is improved. Continuous metabolic support and structure protection are provided for isolated organs, and the organ preservation quality and the success rate of subsequent transplantation are improved.

Methods and kits for screening, diagnosing, detecting or predicting a patient outcome / risk variable for a lung transplant recipient after transplant or an EVLP outcome by measuring biomarker levels of one or more biomarkers selected from IL-6, IL-8, sTNFR1 and sTREM-1 in EVLP perfusate are described. The methods involve for example,i. obtaining one or more test EVLP perfusate samples of a donor lung;ii. determining in one or more test EVLP perfusate sample of a donor lung, a polypeptide level of one or more biomarkers selected from IL-8, IL-6, sTNFR1 and sTREM-1; andiii. a) comparing the polypeptide level of the one or more biomarkers in the perfusate sample with a control or cut-off level, wherein the differential level is indicative of outcome / risk of after transplant or of an EVLP outcome; orb) using the polypeptide level of one or several of the one or more biomarkers in combination, as part of an algebraic calculation of outcome / risk.

This utility model discloses a cranial flap resuscitation and perfusion device, including a workbench with a platform plate fixedly connected to the top of the workbench; a perfusion mechanism is provided on the top of the platform plate, and a sealing component is provided inside the perfusion mechanism; wherein, the perfusion mechanism includes an insulated box fixedly installed on one side of the top of the platform plate, and an inner liner is embedded in the inner side of the insulated box, and a liquid addition pipe is fixedly connected between the top of the insulated box and the inner liner, thereby enabling precise control of the temperature, flow rate and pressure of the perfusion fluid, simulating the human physiological environment, providing good resuscitation conditions for the isolated cranial flap, effectively maintaining the activity of cranial flap bone cells, improving the survival rate of cranial flap after reimplantation, reducing the incidence of postoperative complications such as bone flap necrosis and infection, greatly improving the efficiency of cranial flap repair and cleaning, and reducing the workload of staff.

Ablation electrode assemblies include an inner core member and an outer shell surrounding the inner core member. The inner core member and the outer shell define a space or separation region therebetween. The inner core member is constructed from a thermally insulative material having a reduced thermal conductivity. In an embodiment, the space is a sealed or evacuated region. In other embodiments, irrigation fluid flows within the space. The ablation electrode assembly further includes at least one thermal sensor in some embodiments. Methods for providing irrigation fluid during cardiac ablation of targeted tissue are disclosed that include calculating the energy delivered to irrigation fluid as it flows within the ablation electrode assembly through temperature measurement of the irrigation fluid. Pulsatile flow of irrigation fluid can be utilized in some embodiments of the disclosure.

Provided is a method for culturing a sample of cells, which includes circulating epithelial cells, peripheral blood mononuclear cells (PBMC), or body fluid-derived cell samples (e.g. blood, ascites, pleural effusions, cerebrospinal fluids, perfusion fluids, sputum, and urine), or liquid biopsy-derived cell samples in presence of Bone Morphogentic Protein (BMP) inhibitor, a mitogenic growth factor, and a Wnt agonist, when culturing the circulating epithelial cells and relevant blood cells. Also provided is use of cell medium having a BMP inhibitor, a mitogenic growth factor, and a Wnt agonist in obtaining a cell culture of circulating epithelial cells, PBMC and body fluid-derived cells.

Provided herein are perfusate compositions, and methods and systems for using such composition to perfuse a vascularized composite tissue (e.g., limb, face, abdominal wall or flap) to preserve such composite tissues (e.g., for auto or allotransplant). In certain embodiments, the perfusate compositions comprise at least one of the following reagents: i) N-acetylcysteine; ii) pantothenate or pantothenic acid at a concentration of at least 15 micromol / liter in said composition; and / or iii) carnitine, as well as further comprising one of the following: i) at least one bicarbonate / CO2-dependent anaplerotic substrate, and at least one bicarbonate / CO2 buffer, or ii) at least one non-bicarbonate / CO2-dependent anaplerotic substrate. In certain embodiments, the perfusate compositions are free, or detectably free, of alpha amino acids.

The present application relates to the technical field of interventional medical instruments, in particular to a pressure-controllable super-high-oxygen myocardial perfusion device and a pressure control method. The device comprises a normal-pressure oxygenation cavity, a heat exchange cavity and a high-pressure oxygenation cavity connected in sequence, and a main circulation loop and a static high-pressure oxygenation bypass; the inlet and outlet of the high-pressure oxygenation cavity are both provided with a blockage type peristaltic pump with pumping and sealing functions, the peristaltic pump is internally provided with an oxygenation membrane, and is configured with a pressurizing mechanism for simultaneously pressurizing liquid sides and oxygen sides on both sides of the oxygenation membrane, and a heat exchange mechanism for cooling the perfusion liquid to about 14 DEG C. The present application can prepare super-high-oxygen myocardial protective liquid with oxygen partial pressure higher than 700 mmHg through static, low-temperature and high-pressure oxygenation while maintaining extracorporeal circulation continuous perfusion, significantly improve the myocardial protection effect, and has the advantages of compact structure, precise control and safe operation.

A phacoemulsification system and a method are described. The system including: a handpiece having a needle and a sleeve at a distal end and an orientation detection unit; an irrigation module configured to supply irrigation fluid into the eye; an aspiration module configured for aspiring eye fluid; a priming cover for fitting over the distal end of the handpiece during a priming process; external pressure sensors coupled with the priming cover; one or more sensors for monitoring at irrigation and spiration pressure; and a processor configured to operate in a priming process. The priming process includes operating the irrigation module to fill the irrigation channel with fluid and remove air bubbles. The one or more processors are configured for obtaining calibration pressure data from the external pressure sensors and orientation data from the orientation detection unit and for calibrating the sensors of the system in accordance with orientation data.