2505 results about "Arginine" patented technology

A pharmaceutical composition comprising a protein arginine methyltransferase 5 (PRMT5) inhibitor and a KRASG12C (Kirsten rat sarcoma viral oncogene homolog glycine-to-cysteine) inhibitor. The pharmaceutical composition can be used for treating various cancers, comprising solid tumors. The combined product can be used for treating any number of diseases associated with PRMT5 and / or KRASG12C.

The invention discloses a scalp essence capable of controlling oil and removing dandruff and a preparation method thereof, and provides the scalp essence capable of controlling oil and removing dandruff. The formula consists of the following raw materials: water, saccharomycetes / rice fermentation product filtrate, butanediol, phenoxyethanol, caprylic acid / capric triglyceride, galactose yeast-like bacterium fermentation product filtrate, allantoin, a saccharomycetes / zinc fermentation product, nicotinamide, arginine, polysorbate-20, hydrogenated lecithin, acrylic acid (ester) / C10-30 and an alkanol acrylate cross-linked polymer. Hydrolyzed sodium hyaluronate and trace components. According to the oil-controlling and dandruff-removing scalp essence disclosed by the invention, through the synergistic interaction among the raw materials, the effects of replenishing water, preserving moisture, controlling oil and removing dandruff are increased, the effects of relieving, recovering oil-water balance, recovering skin elasticity and delaying senescence are also achieved, the effects of preventing hair loss and nourishing hair are also achieved, and meanwhile, the oil-controlling and dandruff-removing scalp essence has the advantage of being stable in storage.

The present invention relates to compounds used as MTA-cooperative inhibitors of protein arginine N-methyl transferase 5 (PRMT5). In particular, the present invention relates to MTA-cooperative PRMT5 inhibitors, including their pharmaceutical compositions comprising the compounds and the methods of use thereof.

The present disclosure describes pharmaceutically stable high concentration liquid formulations of antibody. Such formulations comprise, in addition to the antibody, at least one anti-aggregating agent selected from arginine or lysine, buffer and poloxamer 188. In addition, the present disclosure provides high concentrated antibody formulation having high monomer, low aggregates and desirable viscosity.

The invention belongs to the technical field of metal organic frameworks, and particularly relates to a high-performance photocatalytic metal organic framework antibacterial material as well as a preparation method and application thereof. The preparation method comprises the following steps of: growing a metal organic framework UiO 66 which takes a cerium element as a metal node on a single-layer graphene oxide UiO66 (at) GO in situ, loading arginine small molecules in the MOF framework to obtain a high-performance photocatalytic MOF antibacterial material UiO66 (at) GO (at) arg, forming a nano hydrogel with a stable three-dimensional structure with chitosan, and performing antibacterial treatment by using the nano hydrogel as a wound dressing. The Ce-based MOF composite QCS / GO-UiO 66-coated arg / PNIPAm hydrogel prepared by the preparation method disclosed by the invention has excellent properties such as elasticity, adhesive power, water absorption and biocompatibility; and under illumination, the photocatalyst has an excellent photocatalytic sterilization effect, and has a good killing effect on escherichia coli and staphylococcus aureus.

The invention discloses recombinant escherichia coli for inactivating or weakly expressing SPMB family inner membrane protein and application of the recombinant escherichia coli in production of L-amino acid. The invention relates to the technical field of gene engineering, and provides recombinant escherichia coli, and SPMB family inner membrane protein in the recombinant escherichia coli is inactivated or reduced in activity. According to the invention, the yjiG gene (coding gene of SPMB family inner membrane protein) in escherichia coli is knocked out, so that the expression intensity of the yjiG gene is weakened, and fermentation production strains of L-threonine, L-tryptophan, L-arginine and L-valine, which are more cost-effective, can be produced. The invention has important significance for improving the fermentation yield of bacteria, namely L-threonine, L-tryptophan, L-arginine and L-valine.

The invention belongs to the technical field of protein engineering, and discloses a Brazilian sweet protein mutant and application thereof. According to the invention, lysine is used for replacing aspartic acid at the 29th site of Brazilian sweet protein, and / or arginine is used for replacing glutamic acid at the 36th site, and / or alanine is used for replacing aspartic acid at the 50th site, amino acid mutation at the sites is carried out to construct a Brazilian sweet protein mutant, and the Brazilian sweet protein mutant is subjected to heterologous expression in pichia pastoris GS115, so that the Brazilian sweet protein mutant is obtained. And carrying out sweetness performance evaluation on the expressed Brazilian sweet protein mutant. The sweetness property of the double mutant Asp29Lys-Asp50Ala protein is improved by 240% compared with the sweetness property of the Brazilian sweet protein before mutation.

The invention discloses an arginine decarboxylase diaA enzyme mutant and application thereof in preparation of butanediamine, and belongs to the field of bioengineering. According to the invention, rational charge overturning transformation is simultaneously carried out on a pentamer meridian oligomeric interface and a dimer latitudinal oligomeric interface, so that stable assembly and efficient catalysis of the decamer under the condition of neutral to alkaline pH (7.0-9.0) are realized. Wherein positive charges are introduced into a meridian interface to weaken electrostatic repulsion, and negative charges are introduced into a latitudinal interface to enhance dimer compactness and substrate transfer efficiency. The specific enzyme activity of the representative double mutant AdiAD110K / H736E at pH 8.0 is about 35 times that of a wild type, and the representative double mutant AdiAD110K / H736E keeps a complete decamer state in a pH range of 7.0-9.0. The yield of butanediamine is up to 145.9 g / L under the whole-cell catalysis of the mutant.

The invention belongs to antibacterial materials, and particularly relates to antibacterial modified waterborne polyurethane and a preparation method and application thereof.The preparation method of the antibacterial modified waterborne polyurethane comprises the following steps that polyol and diisocyanate are mixed and subjected to a prepolymerization reaction, and a polyurethane prepolymer is obtained; mixing the polyurethane prepolymer and a chain extender, and carrying out chain extension reaction; in the chain extension reaction, adding the graft and carrying out esterification reaction to obtain a first intermediate; mixing the first intermediate with triethylamine and carrying out neutralization reaction to obtain a second intermediate; and emulsifying the second intermediate in deionized water to obtain the antibacterial modified waterborne polyurethane. Compared with the prior art, the antibacterial waterborne polyurethane has the advantages that the problems of poor intermiscibility, low durability and the like in physical addition and the problems of limited binding objects and weak antibacterial effect in covalent binding in the antibacterial waterborne polyurethane in the prior art are solved. According to the scheme, arginine and chitosan are grafted to a molecular chain of waterborne polyurethane, so that the waterborne polyurethane is endowed with a synergistic antibacterial property.

The invention provides a pumpkin seed protein peptide for improving vascular endothelial cell dysfunction and a preparation method thereof, and relates to the technical field of bioactive peptides, the pumpkin seed protein peptide is obtained by enzymolysis of pumpkin seed crude protein with compound protease; the compound protease comprises alkaline protease and trypsin; the mass ratio of the alkaline protease to the trypsin is (1: 3)-(3: 1), and compared with other enzymolysis modes, the pumpkin seed protein peptide prepared by performing enzymolysis on the pumpkin seed crude protein by adopting the alkaline protease and the trypsin has the advantage that the capability of scavenging peroxide free radicals is improved; and the arginine is enriched. The form of peptide in the pumpkin seed protein peptide conforms to the characteristics of high-activity antihypertensive active peptide; the traditional Chinese medicine composition has a relatively good improvement effect on vascular endothelial cell dysfunction and can promote the generation of NO factors. The technical problem that in the prior art, no bioactive peptide capable of improving vascular endothelial cell dysfunction exists is solved.

The present invention belongs to the technical field of bioengineering. Provided are an IscB mutein and the use thereof. Compared to the wild-type IscB protein, the IscB mutein comprises arginine at at least one of the following amino acid positions: position 84, position 96, position 102, position 111, position 159, position 368 and position 386; preferably, the IscB mutein further comprises arginine at position 401 and position 456. The relatively strong interaction between the IscB mutein and a double-stranded DNA target improves the editing activity for mammalian cell lines, which can greatly enhance the application potential of the IscB mutein as a new-generation underlying tool for gene editing, and promote the use of the IscB mutein in the aspects of precision medicine, animal model construction and crop breeding.

The invention belongs to the field of skin care products, and particularly relates to an anti-wrinkle and anti-aging composition as well as a preparation method and application thereof, and the anti-wrinkle and anti-aging composition comprises neurotransmitter inhibition peptide, signal peptide, madecassoside, inositol and glycosylglycerol. The neurotransmitter inhibition type peptide is at least three of acetyl hexapeptide-8, arginine / lysine polypeptide, dipeptide diaminobutyrylbenzylamide diacetate and acetyl octapeptide-3, and the signal type peptide is at least two of palmitoyl pentapeptide-4, decapeptide-4 and acetyl tetrapeptide-9. Compared with the prior art, all the components of the anti-wrinkle and anti-aging composition act on different anti-aging pathways and can be matched with one another from multiple aspects of oxidation resistance, inflammation resistance, moisturizing, cell viability enhancement, wrinkle reduction and the like, a more comprehensive and better anti-aging effect is provided, skin aging is comprehensively resisted, the skin is in a more young state, and the anti-wrinkle and anti-aging composition has the advantages that the anti-wrinkle and anti-aging effects are achieved. And the anti-wrinkle and anti-aging composition is small in irritation and good in stability.

The present invention relates to magnetic, catalytically active, and radiosensitizing nanoparticles (NanoXcats) and the method for their production. A method is disclosed for the production of functionalized greigite nanoparticles with a narrowband size distribution (10-30 nm) for use in multimodal cancer therapy. NanoXcats are superparamagnetic Fe3S4 nanoparticles whose surfaces are equipped with polar functional groups. The surface structures of NanoXcats are suitable for conjugation with, or for covalent or electrostatic binding of, active substances, which may be cancer drugs, cell growth inhibitors, or NO donors. Due to the EPR effect, NanoXcats are preferentially internalized by tumor cells. The targeted accumulation of NanoXcats in tumor tissue is achieved by an external inhomogeneous magnetic field. NanoXcats exhibit enhanced catalytic activity in tumor cells.The catalytic activity of intracellular NanoXcats can be triggered, controlled, and enhanced by low-dose X-rays. Surface-bound drugs are additionally activated in tumor cells by the catalytic activity of NanoXcats. NanoXcats act as superoxide dismutase, nitric oxide synthase, and Fenton catalyst. They catalyze, for example, reactions of the superoxide anion radical to hydrogen peroxide, of L-arginine to NO, and of hydrogen peroxide to the hydroxyl radical.

The invention provides a collagen freeze-dried product and a preparation method thereof, and belongs to the technical field of biology. In the formula design, through the synergistic effect of the arginine (salt) and the surfactant, denaturation and aggregation of the collagen freeze-dried product in the production process and the storage period are inhibited, the stability of the product is improved, and the prepared collagen freeze-dried product is high in activity, purity and safety. In the freeze-drying process, pre-freezing parameters are optimized, and the nucleation effect of ice crystals in the freezing process of the collagen mixed solution is controlled by regulating and controlling parameters such as temperature and vacuum degree in the freeze-drying process, so that the shapes and the sizes of the crystals in the collagen freeze-dried product are more uniform, the drying time is shortened, and the production efficiency is improved. And the collagen freeze-dried product with uniform pore diameter and excellent appearance is obtained, and the uniformity and stability of batch and inter-batch products are improved.

The invention discloses arginine methyltransferase protein from phytophthora plant pathogenic oomycetes as well as a coding gene and application thereof. The arginine methyltransferase protein provided by the invention is as shown in sequences 6-8; the coding gene is shown in sequences 1-3. Experiments prove that the protein disclosed by the invention plays an important role in growth, development and pathopoiesis processes of typical phytophthora plant pathogen oomycete phytophthora capsici, and after the protein is deleted or the expression level is reduced, the growth rate of phytophthora capsici hyphae is reduced, the sporangium yield is reduced, the number of zoospore is reduced, the pathogenicity is reduced and the like. Corresponding arginine methyltransferase inhibitors have different degrees of activity on typical plant pathogenic oomycetes such as phytophthora capsici, phytophthora sojae and phytophthora infestans. The gene provided by the invention has great application potential in the aspect of preventing and controlling the crop epidemic diseases caused by phytophthora plant pathogenic oomycetes, and a novel bactericide developed based on the protein as an action target has important practical significance in controlling the occurrence and prevalence of the crop epidemic diseases caused by phytophthora.

The invention relates to hydrogel loaded with oxygen self-producing nanoparticles as well as a preparation method and application of the hydrogel. The preparation method comprises the following steps: 1, taking cross-linked methacrylamide silk fibroin and hyaluronic acid modified by arginine-glycine-aspartic acid peptide as base materials, and loading self-oxygen-producing nano particles CaO2 (at) PVP (at) Cu-EGCG, and the self-oxygen-producing nano particles CaO2 (at) PVP (at) Cu-EGCG are prepared by coating Cu-EGCG on calcium peroxide nano particles modified by PVP modified polyvinylpyrrolidone. The hydrogel can improve energy metabolism and regulate immune environment through dual mechanisms, realizes efficient promotion of myocardial repair, and provides a new strategy and thought for tissue regeneration and functional recovery after myocardial infarction.

The invention discloses a full D-type antibacterial peptide with high enzymolysis stability and broad-spectrum antibacterial activity and application thereof. The antibacterial peptide is obtained by sequentially and repeatedly arranging D-type tryptophan, D-type arginine and D-type lysine for four times and carrying out C-terminal amidation; the amino acid sequence of the gene is (D-Trp)-(D-Arg)-(D-Lys)-(D-Trp)-(D-Arg)-(D-Lys)-(D-Trp)-(D-Arg)-(D-Lys)-(D-Trp)-(D-Arg)-(D-Lys)-(D-Trp)-(D-Arg)-(D-Lys)-NH2, and is marked as DWRK-12. The antibacterial peptide shows broad-spectrum antibacterial activity and excellent stability, can effectively resist clinically separated multidrug resistant strains, and keeps high cell selectivity at the same time. Due to the characteristics, the antibacterial peptide has important application value in the aspect of developing novel antibacterial drugs, especially in the field of treatment of infection of multiple drug-resistant bacteria.

The invention belongs to the field of biological medicine, and particularly relates to a respiratory syncytial virus F protein mutant and application thereof. The respiratory syncytial virus F protein mutant is characterized in that at least one pair of amino acid residues in an F1 subunit and / or an F2 subunit of F protein polypeptide are substituted by cysteine, and / or an F protein signal peptide part is substituted by a human immunoglobulin light chain signal peptide, and / or alanine A at the 107 site of a multi-base sequence is mutated into arginine R, lysine K at the 134 site is mutated into arginine R, and the amino acid residues in the F1 subunit and / or the F2 subunit of the F protein polypeptide are mutated into arginine. According to the invention, more neutralizing antibody epitopes can be exposed, the stable pre-fusion conformation is maintained, effective neutralizing antibody reaction and antibody binding reaction to the RSV subtypes A and B can be ensured, and the vaccine is suitable for various vaccine forms taking the RSV F protein as the antigen.

The invention discloses a formate dehydrogenase mutant and application of the formate dehydrogenase mutant in catalysis of carbon dioxide reduction, the 223 arginine (Arg) of an amino acid sequence of formate dehydrogenase PsFDH48 derived from Paracoccus sp. MKU1 is mutated into proline (Pro) or the 242 proline (Pro) is mutated into phenylalanine (Phe) through a site-specific mutagenesis technology, and the obtained mutants R223P and P242F have higher catalytic activity and can be applied to catalysis of carbon dioxide reduction. The catalyst can catalyze CO2 to generate formic acid more effectively in vitro, and has potential application value in the aspects of efficient activation of CO2 and further conversion of CO2 into other mono-carbon compounds through cascade reaction.

The invention relates to the field of polypeptide compositions, in particular to a polypeptide metal chelate and application thereof in the field of cosmetics, and the polypeptide metal chelate is formed by chelating a polypeptide compound and a metal ion compound. The structure of the polypeptide compound is [gamma-Glu] n-AA, n is greater than or equal to 1, and AA is selected from glycine, alanine, valine, leucine, isoleucine, methionine (methionine), proline, tryptophan, serine, tyrosine, cysteine, phenylalanine, asparagine, glutamine, threonine, asparaginic acid, glutamic acid, lysine, arginine or histidine; the polypeptide chelated zinc prepared by the invention has remarkable oil control and anti-inflammatory effects, and can be compounded with other raw materials to form cosmetics with oil control and acne removal effects.

The invention discloses antibacterial and antioxidant composite hydrogel as well as a preparation method and application thereof. The antibacterial and antioxidant composite hydrogel is hydrogel formed by interpenetrating and cross-linking zinc ion loaded hectorite, gallic acid grafted methacrylated gelatin and arginine grafted methacrylated chitosan, the preparation method comprises the following steps: by taking gelatin and chitosan with good biocompatibility as substrate materials, carrying out grafting modification to obtain gallic acid-methacrylated gelatin (gallic acid grafted methacrylated gelatin) and arginine-methacrylated chitosan (arginine grafted methacrylated chitosan); the preparation method comprises the following steps: firstly, preparing a hydrogel, then mixing the hydrogel with zinc-hectorite (hectorite loaded with zinc ions) to prepare a stock solution, carrying out photo-crosslinking to form the hydrogel, and the prepared hydrogel has good antibacterial property, oxidation resistance, biocompatibility and blood compatibility, and can promote cell growth and wound healing and reduce inflammatory response.

The invention discloses a cyclic nonapeptide with repairing and anti-oxidation effects and application of the cyclic nonapeptide, the amino acid sequence of the cyclic nonapeptide is cyclic (arginine-glycine-aspartic acid-serine-arginine-lysine-valine-lysine-serine), the prepared cyclic nonapeptide has the repairing and anti-oxidation effects, and the cyclic nonapeptide has the repairing and anti-oxidation effects. The composition can be applied to preparation of cosmetics with repairing and / or anti-oxidation effects.

The invention relates to a multi-effect bionic liposome transdermal repair carrier and a preparation method and application thereof.The multi-effect bionic liposome transdermal repair carrier comprises active ingredients and a nano-carrier, and the active ingredients comprise sialic acid, saccharomycetes / rice fermentation product filtrate, arginine / lysine polypeptide and vitamin E in the mass ratio of (1-10): (2-8): (0.1-1): (0.5-4); the nano-carrier is prepared from the following raw materials: phospholipid, an emulsifier, polyhydric alcohol and water. Compared with the prior art, the anti-aging, whitening and moisturizing composition has the advantages of enhancing skin moisturizing and anti-oxidation effects, promoting collagen synthesis and inhibiting tyrosinase to achieve remarkable anti-aging, whitening and moisturizing effects and the like.

The invention provides lipidosome wrapping L-arginine / Prussian blue nano particles and a preparation method and application thereof, and belongs to the technical field of biological agents. L-arginine is deposited on hPB through iron chelation, then the lipidosome is coated to avoid leakage of water-soluble L-arginine in circulation, and thus the L-arginine / Prussian blue nano particles are obtained. The liposome coated with the L-arginine / Prussian blue nano particles is obtained. Due to the EPR effect, the hPLL-coated Lipo can be efficiently transferred and distributed into tumor tissues, heat is generated under the near-infrared light irradiation condition to ablate a large number of tumor cells, the utilization degree of the tumor cells to L-arginine is greatly limited, meanwhile, immunogenic death is induced, DAMPS is released in situ, infiltration and activation of anti-tumor T cells are further promoted, and the anti-tumor effect is achieved. The tumor immunotherapy is enhanced. And under illumination, the liposome disintegrates and releases the L-arginine, so that nutritional support is provided for immunological competence cells in competitive disadvantage, and activation of an immune microenvironment is promoted.

Described herein are compounds, compositions, and methods for diagnosing and / or monitoring pathogenic disease using positron emission tomography. Also described are conjugates of the formula B-L-P, wherein B is a radical of a targeting agent selected from vitamin receptor binding ligands (such as folate), PSMA binding ligands, or PSMA inhibitors; L is a divalent linker comprising aspartic acid, lysine, or arginine, and P is a radical of an imaging agent or radiotherapy agent, such as a radionuclide or radionuclide containing group, or a radical of a compound capable of binding a radionuclide, such as a metal chelating group.

The invention relates to bionic skin barrier cream with a three-layer phospholipid structure and a preparation method of the bionic skin barrier cream, and belongs to the technical field of cosmetics, the bionic skin barrier cream comprises the following raw materials: lecithin, wax ester, triglyceride, composite ceramide, phytosterol oleate, squalane, an antioxidant, polyol, composite amino acid, pyrrolidone carboxylic acid, urea and arginine. A stabilizing agent, a treating agent and the balance of deionized water. The bionic skin barrier cream prepared by the invention has a three-layer phospholipid structure, and compared with a double-layer phospholipid structure in the market, the firmness and hydrophobicity of the double-layer structure are further improved by the added third-layer structure, so that the prepared bionic skin barrier cream has outstanding waterproof, sweat-resistant and light pollution-resistant effects; in addition, the wax ester, the triglyceride, the phytosterol oleate, the pyrrolidone carboxylic acid and the urea selected in the invention have a synergistic effect, and can generate excellent moisturizing and repairing effects and certain protection effects.

The invention discloses an anti-hair-loss composition, preparation thereof and application of the anti-hair-loss composition in hair washing and caring products, and belongs to the field of cosmetic preparation. The method for preparing the anti-hair-loss composition with the good and lasting anti-hair-loss effect comprises the following steps: (1) uniformly mixing arginine and citric acid, then adding diaminopyrimidine oxide, and continuously and uniformly mixing to form a mixture A; (2) uniformly mixing azelaic acid, nicotinamide and butanediol to form a mixture B; (3) uniformly mixing the mixture A, the mixture B, caffeine, a sanguisorba officinalis root extract, a green tea extract and a cacumen biotae extract to obtain the anti-hair loss composition. The anti-hair loss composition prepared by the invention is simple in component and simple in preparation process; the effect is lasting, and the anti-hair loss effect is good.

The invention discloses a preparation method of perindopril amlodipine tablets, and belongs to the field of pharmacy. The perindopril amlodipine tablet is prepared from the following raw material medicines: arginine perindopril and amlodipine besylate and auxiliary materials: lactose, microcrystalline cellulose, hydrophobic colloidal silicon dioxide and magnesium stearate. The preparation method of the perindopril amlodipine tablet comprises the following steps: weighing according to the prescription amount, crushing and sieving the raw materials for pretreatment, sieving the whole particles of the auxiliary materials for pretreatment, premixing, feeding and total mixing, tabletting and the like. The prepared perindopril amlodipine tablet is relatively good in quality, high in stability and relatively few in waste products.

The compositions comprise at least two ingredients selected from the group consisting of fish oil, L-arginine, vitamin E, vitamin C, vitamin B3, vitamin B5, vitamin B6, vitamin B1, vitamin B2, folic acid, biotin, vitamin B12, and are formulated for the prevention and / or treatment of a disease or condition associated with muscle decline in a subject in need thereof. Preferably, the subject in need is an individual at risk of or having renal failure, renal dysfunction or / and nephropathy, such as chronic nephropathy, protein energy consumption, end-stage nephropathy, renal failure due to hospitalization in an intensive care unit, acute kidney injury, and the muscle decline is selected from the group consisting of muscle depletion, muscle loss due to renal failure or dysfunction, muscle decline associated with chronic kidney disease, muscle loss due to hospitalization in intensive care units during renal failure, metabolic acidosis during chronic kidney disease, protein energy depletion, or a combination thereof. The method enables the prevention and / or treatment of at least one outcome of a disease or condition associated with muscle decline in a subject at risk of or having renal failure, renal dysfunction or / and kidney disease. The composition and the method can be used to improve the level of at least one amino acid.

The invention relates to the technical field of medicine, in particular to a crystal form of (S)-2-(1-acetoxy n-amyl) benzoic acid L-arginine salt and a preparation method and application thereof.The crystal form is radiated through Cu-K alpha, an X-ray powder diffraction pattern expressed by the diffraction angle 2 theta is obtained, the diffraction angle 2 theta has characteristic peaks at the positions of 5.10, 10.23, 14.13, 17.58, 19.81, 20.63 and 21.73, and the crystal form is shown in the description. And the error range of each characteristic peak 2 theta is + / -0.2. The technical scheme provided by the invention has the following advantages: research shows that the I crystal form of the compound as shown in the formula I prepared by the invention is good in stability and relatively high in purity, and a single crystal of the I crystal form is obtained; the crystal form I of the compound shown in the formula I can meet the medicinal requirements of production, transportation and storage, the production process is stable, repeatable and controllable, and the crystal form I can be suitable for industrial production.