58 results about "Catabolism" patented technology

Particulated and reconstituted tissues comprising small, densely packed tissue microparticles encapsulated in a tissue specific promoting gel packed at a percolation threshold that can be transplanted into damaged tissue thereby facilitating regeneration following trauma to the tissue. The engineered microparticle construct for tissue replacement and repair, as taught herein, provides numerous benefits including (1) encouraging a regenerative response in damaged tissue regions, (2) mimicking the structural support of native tissue, (3) establishing an environment that promotes attachment, migration, and differentiation of infiltrating stem cells, and (4) providing a source of growth factors and other anti-catabolic growth factors and cytokines. Tissue specific microparticles packed together at, or past, their percolation threshold will provide the necessary mechanical environment and to best recapitulate and integrate with native tissue. The packing of microparticles, derived from the ECM of native tissue to a concentration past the percolation point will yield both the necessary biochemical and biomechanical properties necessary for reconstituting a specific tissue.

The invention discloses application of thiamine in preparation of a product for treating subclinical ketosis of dairy cows, and belongs to the field of biological medicines. Tests prove that compared with dairy cows suffering from subclinical ketosis (SCK), by adding thiamine, the content of beta-hydroxybutyric acid (BHBA), the number of bacteria in milk and the number of somatic cells can be remarkably reduced, and the milk yield, the milk fat content, the acetic acid content and the A / P ratio are remarkably increased; the microbial diversity of rumen and excrement of the dairy cow can be adjusted; a metabonomics result shows that metabolite up-regulated after the thiamine is added mainly focuses on pyruvic acid metabolism, glycolysis or gluconeogenesis, tyrosine metabolism, glycerophospholipid metabolism and biosynthetic pathways of phenylalanine, tyrosine and tryptophan. The conclusion is that when thiamine is added into the SCK dairy cow, synthesis and catabolism of BHBA of an organism can be effectively regulated and controlled by adjusting the content of succinyl-coenzyme A, then subclinical ketosis is relieved, rumen fermentation is promoted, and therefore the milk yield and the milk quality are improved.

The present disclosure relates to a kit. The kit of the present disclosure comprises: a kit body comprising a needle tube and a needle, the first end of the needle being mounted to the needle tube; and a catabolic filling thread comprising a first segment and a second segment connected to each other, at least a part of the first segment being located inside the needle, and at least a part of the second segment being located outside the needle, wherein the catabolic filling thread is a thread made of poly-L-lactic acid. The kit of the present disclosure comprises a catabolic filling thread which can be composed of poly-L-lactic acid, is absorbable, has no residue, and is metabolized into lactic acid in the human body. Such a configuration makes the catabolic filling thread itself not only play a role in filling and tightening when filling surgery is performed using the catabolic filling thread of the present disclosure, but also the lactic acid after its metabolism can still stimulate fibroblasts in the skin and subcutaneous tissue to reconstruct the collagen framework in the human body by using fibroblasts.

The present disclosure relates to the use of a maltose dependent degron to control stability of a protein of interest fused thereto at the post-translational level. The present disclosure also relates to the use of a maltose dependent degron in combination with a maltose-responsive promoter to control gene expression at the transcriptional level and to control protein stability at the post-translational level. The present disclosure also relates to the use of a stabilization construct that couples expression of a cell-growth-affecting protein with the production of non-catabolic compounds. The present disclosure further relates to the use of a synthetic maltose-responsive promoter. The present disclosure further provides compositions and methods for using a maltose dependent degron, a maltose-responsive promoter, and a stabilization construct, either alone or in various combinations, for the production of non-catabolic compounds in genetically modified host cells.

The invention belongs to the technical field of biological medicines, and particularly relates to application of miR-206-3p in regulation and control of skeletal muscle atrophy, and the change condition of the miR-206-3p level is measured by constructing an in-vitro cancer cachexia myotubular atrophy model; it is verified that in-vitro addition of miR-206-3p mimics relieves in-vitro myotube atrophy induced by a cancer conditioned culture medium, inhibits expression of a myotube marker gene and inhibits proteolysis and metabolism pathways and cell apoptosis of myotubes, so that myotube atrophy induced by cancer cachexia is inhibited. The invention opens up a new thought for the research of cachexia pathogenesis, and also provides a potential new target for the research and development of cachexia treatment and / or cachexia prevention drugs.

Tumor-selective expression of therapeutic molecules by bacteria is achieved by one or more AND, NOR, OR, NOT and / or NAND gate genetic circuits. The therapeutic molecules can be proteins, metabolites or catabolites, and may also be immunotherapeutics or immunotherapeutic cytotoxins. Single or multiple expression components may be used. Tumor selective expression of multimeric proteins utilizes multimerization as to complete the genetic circuit. Genetic circuits that are unlinked, function to achieve a combined effect on specificity of delivery of antitumor therapeutic molecules. Compositions and methods to generate silica, PEG and Poly-HPMA coated bacteria are also provided. Compositions and methods for selectively sensing or imaging tumors are also described.

A method of breaking down at least one component of a substrate can include providing a catabolic nanocompartment that includes a liquid coacervate phase, at least one catalyst within the coacervate phase, and an aqueous phase surrounding the coacervate phase. The method can include contacting the catabolic nanocompartment with the substrate including the at least one component such that the catalyst degrades the at least one component and forms degradation products of the at least one component.

The invention provides recombinant cinnamon streptomyces and application thereof in monensin production, and belongs to the technical field of microbial fermentation and antibiotic preparation. According to the recombinant cinnamon streptomyces provided by the invention, a ccr gene with a nucleotide sequence shown as SEQ ID NO.2 in the cinnamon streptomyces is knocked out, and an xylA gene with a nucleotide sequence shown as SEQ ID NO.1 is overexpressed. According to the recombinant cinnamon streptomyces provided by the invention, through overexpression of the xylA gene, the metabolic capability of the cinnamon streptomyces to xylose is enhanced; the ccr gene is knocked out, so that the carbon catabolism inhibition phenomenon is weakened, and the xylose utilization rate is increased when the recombinant strain takes xylose as a carbon source. The recombinant streptomyces cinnamomi provided by the invention can improve the titer of monensin, reduce the production cost, shorten the fermentation period and improve the production efficiency.

The present disclosure provides an engineered nucleic acid sequence comprising a gene cassette, the gene cassette comprising one or more regulator genes, the regulator genes including an iadR gene, and at least three catabolic pathway genes downstream from the one or more regulator genes, the at least three catabolic pathway genes including an iadC gene, an iadD gene, and an iadE gene, wherein the engineered nucleic acid sequence is free of an iadA gene and iadB gene. The disclosure also provides bacteria cells comprising the engineered nucleic acid sequence, wherein the bacteria cell is capable of degrading indole-3-acetic acid. Methods for degrading auxin, controlling auxin, and promoting plant growth and root development are provided, comprising providing the bacteria cell and allowing the bacteria cell to interact with roots of a plant.

This invention discloses a kitten food without palatability enhancers and its preparation method, relating to the field of pet food technology. It comprises the following components: polypeptide chicken meal, soy protein peptides, gluten hydrolysate, lactoferrin, astragalus extract, yam powder, rosemary oil, resveratrol, tributyrate, and rice. By adding substances containing small peptides and plant extracts to the kitten food, this invention provides the body with a large amount of high-quality animal protein without adding any chemically synthesized palatability enhancers, preservatives, or other chemical additives. This effectively promotes the body's decomposition and metabolism of nutrients, promotes the growth of kittens, and also enhances the body's antioxidant capacity and reduces the occurrence of diarrhea.

The invention provides application of pentenedioic acid (GC) in enhancing the anti-tumor effect of T cells through metabolic reprogramming, and belongs to the technical field of biological medicine. It is found that in the tumor development process, GC is supplemented in an exogenous mode, tumor growth can be rapidly inhibited, and the anti-tumor effect depends on a TME regulation and control mechanism. Through GC treatment, the TME immune landscape can be significantly remodeled; gC treatment not only can enhance the effector function of the CD8 + T cells, but also can delay the depletion process of the CD8 + T cells; and the infiltration CD8 + T cells in the tumor of the GC treatment group are obviously increased. Particularly, after the CD8-resistant antibody is used for removing mouse CD8 + T cells, the anti-tumor effect of GC completely disappears, and it is confirmed that the tumor inhibition effect depends on the CD8 + T cells. Therefore, the invention proves that the lysine catabolic metabolite GC, as an immunostimulatory metabolite, can shape the immune activated TME by reactivating the functions of tumor infiltrating CD8 + T cells.

Increased control and efficiency over the wastewater purification can be achieved by allowing to selectively prioritize catabolic over anabolic processes via prioritization of the digestive function of microorganism in the activated sludge. The gas-dispersion return sludge is created using pure oxygen or oxygen containing trace amounts of ozone, which is blended with return sludge to create a mixture of gas and liquid, which is passed through an atomizer or a pump to instantly render the reactive gas to an ultra-fine bubble state. At least a portion of the ultra-fine bubbles dissolve within the sludge, raising oxygen to a critical level within a short time, activating the dormant microorganisms. The microorganisms accumulate enough cATP upon initial encounter with the pollutants to prioritize their digestive function, and when exposed to pollutants present in wastewater, digest the pollutants using biochemical pathways different from the ones used in nature.

The invention relates to an anti-inflammatory and lipid-lowering-based nano preparation for treating fatty liver and a preparation method of the nano preparation. Galactose modified polyethylene glycol-polylactic acid-glycolic acid copolymer (PEG-PLGA) is used as a targeting carrier, and a lipid-lowering drug fenofibrate and an anti-inflammatory drug curcumin are co-loaded; the particle size of the nano preparation is 150-200nm, and the drug encapsulation efficiency is greater than or equal to 85%. Galactose modified PEG-PLGA is adopted as a carrier, galactose can be specifically combined with an asialoglycoprotein receptor (ASGPR) specifically expressed on the surface of liver cells, active targeting of the liver is achieved, the drug enrichment amount of the liver lesion position is remarkably increased, meanwhile, accumulation of drugs in extrahepatic tissue is reduced, extrahepatic toxicity is effectively reduced, and the drug delivery effect is improved. According to the present invention, the anti-inflammatory drug curcumin and the fenofibrate are combined, such that the biological safety of the preparation is improved, the lipid lowering drug fenofibrate and the anti-inflammatory drug curcumin are innovatively co-loaded, and the fenofibrate and the anti-inflammatory drug curcumin form the synergistic effect system: fenofibrate can activate peroxisome proliferator-activated receptor alpha (PPAR alpha), promote liver lipid catabolism, and reduce lipid deposition;

The present disclosure relates to a pectin-containing wastewater treatment method and a treatment device, the method comprising: inputting first pectin wastewater into a biological conversion tank, and performing biological conversion treatment on pectin in the first pectin wastewater based on a pectin-cellulose degradation mixed bacteria to obtain second pectin wastewater containing small molecular structure pectin; wherein the pectin-cellulose degradation mixed bacteria is a mixed bacterial population capable of simultaneously degrading pectin and cellulose, and the biological conversion treatment process is used to change the pectin structure without affecting the chemical oxygen demand corresponding to the first pectin wastewater; inputting the second pectin wastewater into a primary or multi-stage anaerobic reactor for anaerobic decomposition treatment to obtain wastewater with reduced organic matter concentration after pectin decomposition and biogas; and the biogas is used for input into a power supply end or an energy storage end for recycling. In the wastewater treatment process, in-situ treatment and relatively sufficient degradation of pectin are achieved, and the treatment load and treatment efficiency are improved.

A pharmaceutical compositions includes GLP-1 and GLP-1 analogues inducing an anabolic stimulation of chondrocytes and a decreasing in chondrocyte catabolic including a decrease in cartilage matrix loss and cartilage degeneration for use in the treatment of cartilage disease. The composition induces cartilage regeneration and anabolic stimulation of chondrocytes, including chondrocyte proliferation and / or stem cell differentiation into chondrocytes.

The invention provides yeast engineering bacteria for producing L-tryptophan by utilizing methanol carbon source fermentation and application, and belongs to the technical field of biology. According to the engineered yeast, a methanol substrate is coupled with a methanol inducible promoter induction system, so that the gene expression intensity is dynamically matched with the methanol consumption rate, and the problem of regulation lag caused by separation of an inducer and a substrate in a traditional carbon source is solved; in addition, a gene recombination technology is adopted to enhance expression of key genes in the synthesis route of the L-tryptophan, and the biosynthesis efficiency of the L-tryptophan is remarkably improved by increasing the dosage of target genes, enhancing supply of precursor substances and knocking out genes related to catabolism of the tryptophan in chassis cells at the same time. The constructed yeast engineering bacterium has the characteristics of clear metabolic background and strong heterologous expression ability, can be used for synthesizing L-tryptophan from the beginning completely by taking methanol as a carbon source, effectively gets rid of the dependence of a traditional process on grain resources, reduces the production cost, and provides a brand new technical path for industrial production of L-tryptophan.

The application discloses creatine composition for improving exercise endurance and strength, and a preparation method and application thereof. The creatine composition comprises creatine, black pepper extract and oyster peptide. The scheme has the effects of significantly enhancing energy systems, promoting anabolism, inhibiting catabolism, reducing oxidative damage and accelerating recovery through the combination of components, and can be effectively used for improving exercise endurance and strength.

The invention belongs to the technical field of energy consumption promoting compositions, and particularly relates to an energy consumption promoting nutritional composition as well as a preparation method and application thereof. The nutritional composition capable of promoting energy consumption comprises the following raw materials: L-carnitine, taurine and compound B vitamins, wherein the compound B vitamins comprise vitamin B3, vitamin B5, vitamin B6 and vitamin B12. The food nutrition enhancer with definite components is adopted for composition, catabolism of fat is promoted through different action mechanisms, synergistic interaction is achieved, energy supply through fat decomposition is accelerated, the body is in an energy negative balance state, research shows that the food nutrition enhancer contributes to weight losing and body weight reduction of obese people, the fat-body ratio of the body can be reduced, and the fat-body ratio of the body can be reduced. Good application prospects are realized.

The invention discloses a fermented sausage source active peptide as well as a preparation method and application thereof in preparation of medicines for preventing and treating alcoholic liver injury, and belongs to the technical field of biological medicines. The amino acid sequence of the fermented sausage source active peptide comprises one or more of WLKLRPILK and RGPPRPRPAPA. The method disclosed by the invention has the beneficial effects that the bioactive peptide can be efficiently extracted from the minced pork within a relatively short time. The active peptide has a remarkable relieving advantage on alcoholic liver injury, and can inhibit liver fat synthesis increase caused by alcohol intake and promote catabolism of fatty acid through an AMPK pathway. And a peptide fragment with an AMPK activation effect is rapidly screened through computer screening and molecular docking. The method has high reference value for low-cost preparation of the fermented meat-derived active peptide and prevention of alcoholic liver injury, and is suitable for popularization and application.

Disclosed herein are novel cells that are engineered to catabolize a reduced number of acyl-CoA and branched-chain amino acids, and methods for improved cannabinoid production using these cells.In addition, disclosed are cells that are engineered to produce rare cannabinoids, methods for producing these novel rare cannabinoids, and the novel rare cannabinoid compounds thus produced.Some aspects of the disclosure are directed to cells that are engineered to provide increased production of cannabinoids and / or their derivatives compared to control cells.

The present invention relates to methods of producing industrial products from plant lipids, particularly from vegetative parts of plants. In particular, the present invention provides oil products such as biodiesel and synthetic diesel and processes for producing these, as well as plants having an increased level of one or more non-polar lipids such as triacylglycerols and an increased total non-polar lipid content. In one particular embodiment, the present invention relates to combinations of modifications in two or more of lipid handling enzymes, oil body proteins, decreased lipid catabolic enzymes and / or transcription factors regulating lipid biosynthesis to increase the level of one or more non-polar lipids and / or the total non-polar lipid content and / or mono-unsaturated fatty acid content in plants or any part thereof. In an embodiment, the present invention relates to a process for extracting lipids. In another embodiment, the lipid is converted to one or more hydrocarbon products in harvested plant vegetative parts to produce alkyl esters of the fatty acids which are suitable for use as a renewable biodiesel fuel.

The present invention relates to methods of producing industrial products from plant lipids, particularly from vegetative parts of plants. In particular, the present invention provides oil products such as biodiesel and synthetic diesel and processes for producing these, as well as plants having an increased level of one or more non-polar lipids such as triacylglycerols and an increased total non-polar lipid content. In one particular embodiment, the present invention relates to combinations of modifications in two or more of lipid handling enzymes, oil body proteins, decreased lipid catabolic enzymes and / or transcription factors regulating lipid biosynthesis to increase the level of one or more non-polar lipids and / or the total non-polar lipid content and / or mono-unsaturated fatty acid content in plants or any part thereof. In an embodiment, the present invention relates to a process for extracting lipids. In another embodiment, the lipid is converted to one or more hydrocarbon products in harvested plant vegetative parts to produce alkyl esters of the fatty acids which are suitable for use as a renewable biodiesel fuel.

Provided are methods for promoting weight loss and treating and reducing risk of developing obesity related disorders. The methods involve inhibiting expression or function of NFS1, BOLA3, IBA57, or NFU1, or a combination thereof. The methods induce a metabolic shift that causes weight loss in part by increasing lipid catabolism.

The application provides a hydroxyl-substituted beraprost derivative, a synthesis method and application thereof, and belongs to the technical field of drugs.The compound is a coupling compound containing beraprost and a nitric oxide donor.The compound solves the defects of short elimination half-life of beraprost sodium, multiple single-day administration, saturation ceiling effect of curative effect, short metabolic half-life of NO which is a gas and is rapidly decomposed in a solution, and the like, the new compound reduces the original beraprost administration dose and administration frequency, and simultaneously utilizes the relaxation of smooth muscle caused by the release of NO molecules in the body of the compound, so that the two drugs play a synergistic effect through double action, and the effectiveness and safety of the drug are improved.

The present application discloses a rare ginsenoside composition and its application in anti-fatigue and liver protection, and relates to the technical field of biological medicine. The present application provides a rare ginsenoside composition, which is composed of Rh4, F4 and Dehydroprotopanaxatrial II, and the mass ratio of Rh4, F4 and Dehydroprotopanaxatrial II is (2-4):(2-4):1. The rare ginsenoside composition has the effect of significantly relieving physical fatigue, can reduce the decomposition and metabolism of protein to energy, eliminate fatigue, increase the liver glycogen reserve in the body, improve the fatigue resistance, has the effect of liver protection, enhances the antioxidant defense ability of liver tissue, reduces the lipid peroxidation damage, and improves the lipid metabolism disorder of liver tissue.

The invention belongs to the technical field of biological medicines, and particularly relates to application of rubimaillin in preparation of a medicine for delaying intervertebral disc degeneration. Compared with the limitations of inexact conservative treatment effect and high surgical intervention risk in the prior art, the invention reveals for the first time that rubimaillin (MO) can effectively regulate and control a PI3K-AKT / NF-kappa B signal channel through targeted inhibition of EGFR phosphorylation, alleviates nucleus pulposus cell metabolic disorder caused by an inflammatory microenvironment from the source, significantly improves the expression of anabolism markers, and provides a new application for the treatment of the inflammatory microenvironment. The compound inhibits the release of catabolic enzymes and inflammatory factors, delays the processes of cell aging and apoptosis, and shows a clear effect of delaying the progress of IDD in animal and cellular levels. Compared with the traditional treatment, the traditional Chinese medicine composition has the advantages of good effect, low side effect and the like, so that a new way can be provided for preventing and treating the IDD.

The invention discloses a bacillus licheniformis recombinant strain with high yield of L-cysteine, and belongs to the technical field of synthetic biology and microbial metabolism engineering. The cysteine synthesis flux is enhanced through multiple knockout and combined overexpression. An efficient engineering strain is constructed by knocking out a serine hydroxymethyltransferase coding gene glyA and an L-serine ammonolysis enzyme coding gene sdaAA of a serine catabolism key pathway, knocking out a cysteine catabolism gene metC and combining gene overexpression to introduce a feedback inhibition removal mutant cysEf and a transporter eamA, and the efficient engineering strain is used for preparing a recombinant strain. Finally, the yield of the L-cysteine reaches 1.075 g / L in shake flask fermentation (the conversion rate is 18.69%), and the application potential of the bacillus licheniformis in sulfur metabolism engineering is highlighted.

The invention discloses a crucian feed with soybean meal replaced by cottonseed protein concentrate and application of the crucian feed. Cottonseed protein concentrate is used as a main plant protein source and completely replaces soybean meal in a feed formula, and the mass percent of the cottonseed protein concentrate is 19.78%. The feed further comprises the following components: fish meal, casein, flour, fish oil, soybean oil, vitamins, mineral premix and the like. The feed provided by the invention can significantly improve the ingestion speed and the ingestion amount of crucian carps and effectively promote the growth of crucian carps, and the weight gain rate, the specific growth rate and the feed efficiency of the feed are significantly superior to those of a feed containing soybean meal. The feed can regulate and control lipid metabolism related gene expression of crucian muscle and liver, enhance fat synthesis and catabolism, promote muscle protein and fat deposition and increase muscle polyunsaturated fatty acid content, and has no negative influence on liver health and intestinal flora homeostasis. The invention provides an efficient solution for soybean meal decrement substitution, and has important application value.

The invention relates to the field of formula foods for special medical purposes, in particular to a functional nutritional supplement containing calcium beta-hydroxy-beta-methylbutyrate, which comprises the following raw materials in parts by mass: 2.6-7.7 parts of calcium beta-hydroxy-beta-methylbutyrate; 13.8 to 17.2 parts of compound amino acid; 56 to 78.5 parts of whey protein powder; 0.2 to 0.5 part of vitamin; 0.3 to 0.5 part of hydroxy citric acid; 4.9 to 5.5 parts of functional nano particles; 2.3 to 3 parts of a cyclodextrin inclusion compound; 0.9-1.2 parts of a rhizoma alismatis soup polysaccharide composition; 1-3 parts of inulin; 1-3 parts of lotus leaf pectin polysaccharide (LLP); 0.5 to 0.9 part of hydrophilic fumed silica; the functional nanoparticles, the cyclodextrin inclusion compound, the rhizoma alismatis decoction polysaccharide composition and the calcium beta-hydroxy-beta-methylbutyrate have a synergistic effect, anabolism is enhanced, catabolism is inhibited, the sarcopenia is effectively improved, and the composition has a wide application prospect.

The application discloses a penicillium oxalicum engineering bacterium and a construction method thereof. Penicillium oxalicum The engineering bacterium is named penicillium oxalicum (QL-ACxrC-ACreA) and has a preservation number of CCTCC NO: M 2026896. The penicillium oxalicum engineering bacterium is obtained by knocking out transcriptional inhibitor CxrC and transcriptional factor CreA of carbon catabolite repression of filamentous fungi, so that when starch is used as the only carbon source for fermentation, the amylase activity and extracellular protein content generated by the penicillium oxalicum engineering bacterium are significantly improved; and the knocking out of the two factors synergistically improves the starting efficiency of the promoter Pamy15A.