5 results about "Regulon" patented technology

This invention relates to the field of genetic engineering technology, specifically to a recombinant plasmid, its construction method, and its application in enhancing the synthesis of pseudocladocerin from Nostoc flagelliforme. The recombinant plasmid includes a vector plasmid, and sgRNA target sequences, HR homologous sequences, psbA promoter sequences, and other components linked to the vector plasmid. ScyR Regulatory genes. This recombinant plasmid can be used in black moss. MAAs Overexpression of key regulatory genes at gene cluster sites significantly enhanced the pseudocranophane synthesis capacity of Nostoc commune. The Nostoc mutant obtained by transforming this recombinant plasmid into Nostoc commune exhibited a pseudocranophane content 2.4 times higher than that of wild-type Nostoc commune after 15 days of culture.

Genetically modified cells that are compatible with multiple subjects, e.g., universal donor cells, and methods of generating said genetic modified cells are provided herein. The universal donor cells comprise at least one genetic modification within or near at least one gene that encodes one or more MHC-I or MHC-II human leukocyte antigens or component or transcriptional regulator of the MHC-I or MHC-II complex, at least one genetic modification that increases the expression of at least one polynucleotide that encodes a tolerogenic factor, and optionally at least one genetic modification that increases or decreases the expression of at least one gene that encodes a survival factor.

Provided is a method for increasing the productivity of secondary metabolites such as antibiotics in a microorganism. In particular, provided is a method to improve the productivity of secondary metabolites in a microorganism by inactivating or reducing the expression of FadR transcription regulator and Srb RNA binding protein of said microorganism.

Methods for determining regulon enrichment in gene expression signatures are disclosed herein. An example method can include obtaining a set of transcriptional targets of a regulon. The method can include obtaining a gene expression signature by comparing a gene expression profile of a test sample to gene expression profiles of a plurality of samples representing control phenotypes. The method can include calculating a regulon enrichment score for each regulon in the gene expression signature. The method can including determining whether a number of control samples in the control phenotypes is above a predetermined threshold to support evaluation of statistical significance using permutation analysis. The method can include, in response to determining that the number of control samples is above the predetermined threshold, calculating a significance value by comparing each regulon enrichment score to a null model.