16results about "Carbon-nitrogen lyases" patented technology

This invention provides compositions and treatment methods for reducing hyperphenylalaninemia. [Solution] A genetically modified bacterium is provided, comprising: a) one or more genes encoding phenylalanine ammonia lyase (PAL), which are operably linked to a promoter that is not naturally associated with the PAL gene and can be directly or indirectly induced; b) one or more genes encoding a phenylalanine transporter, which are not naturally associated with the phenylalanine transporter gene and can be operably linked to a promoter that is directly or indirectly induceable; and c) one or more genes encoding mutant fumarate and nitrate reductase (FNR), which are not naturally associated with the FNR gene and can be operably linked to a promoter that is directly or indirectly induceable.

The present invention relates to a microorganism transformed to be capable of producing guanidinoacetic acid (GAA) and containing at least one gene encoding a protein having the function of NADH-dependent dehydrogenase, and a method for the fermentative production of GAA using such a microorganism. The present invention also relates to a method for the fermentative production of creatine.

A method for the incorporation of formaldehyde into biomass is described, comprising the following enzymatically catalyzed steps: (1) condensation of pyruvate and formaldehyde to 4-hydroxy-2-oxobutanoic acid (HOB); (2) amination of the resulting 4-hydroxy-2-oxobutanoic acid (HOB) to produce homoserine; (3) conversion of the resulting homoserine to threonine; (4) conversion of the resulting threonine to glycine and acetaldehyde or acetyl-CoA; (5) condensation of the resulting glycine with formaldehyde to produce serine; and (6) conversion of the resulting serine to produce pyruvate, which can then be used as a substrate in step (1).

The present invention relates to enzyme selection systems for glycine amidinotransferase and guanidinoacetate methyltransferase, and the use of these optimized enzyme in fermentation processes. In particular, the present invention relates to the production of creatine, using such enzymes, as well as cells optimized for creatine production.

The present invention relates to novel strains of lactic acid bacteria, more specifically, novel strains of Lactobacillus reuteri, capable of utilizing ethanolamine. This characteristic makes it possible for the bacteria to compete for the same substrate as pathogenic ethanolamine-utilizing pathogens, thereby presenting an effective means to combat infections resulting from such pathogenic bacteria. There is also provided a method of selecting further ethanolamine-utilizing lactic acid bacteria, as well as other methods and uses involving said novel strains and otherethanolamine-utilizing lactic acid bacteria.

The present application provides a recombinant E. coli for synthesizing heme and derivatives thereof, wherein the recombinant E. coli is knocked out of genes such as pyruvate oxidase poxB, pyruvate formate-lyase pflB and protoporphyrinogen peroxidase yfeX, and meanwhile, the genes such as phosphopyruvate carboxylase ppc, glycerol transporter glpF and 3-phosphoglycerate dehydrogenase glpD are enhanced, and the genes such as 5-aminovaleryl-CoA synthase hemA and ferrous chelatase hemH are introduced, so that the recombinant E. coli can synthesize heme and derivatives thereof by using glycerol as a raw material, and has application prospects in the field of biological manufacturing.

This invention provides an enzymatic method for the preparation of R-3-aminobutyric acid (GABA), belonging to the field of biochemistry. Using crotonic acid as a substrate and a recombinant aspartate enzyme mutant as a biocatalyst, an enzymatic reaction is carried out under suitable temperature and pH control to efficiently and stereoselectively prepare the product R-3-aminobutyric acid. This method utilizes *E. coli* as a host for heterologous expression of recombinant aspartate enzyme. The whole cells of the expression host are used as crude enzyme for the enzymatic reaction. After extraction and purification, high-purity R-3-aminobutyric acid is obtained, with a conversion rate exceeding 99% and a product purity >99% and >99.9% ee. This method features high conversion and high yield, and is easily industrialized.

Provided is a promoter, which has a nucleotide sequence shown in SEQ ID NO. 1. The promoter is obtained by means of rationally designing the promoter of a dapA gene, has obviously reduced transcriptional activity and can be used for the weakened expression of microorganism genes. Substituting the original promoters of the dapA gene, a ddh gene, an ilvA gene, a tdcB gene and a gltA gene in Corynebacterium glutamicum by using the promoter enables the expression levels of these genes to be significantly reduced, the carbon metabolic in a recombinant microorganism constructed thereby flows more to a threonine anabolism pathway, and the recombinant microorganism has a significantly improved production of threonine in comparison with an original strain and has good growth performance.

The present invention is in the field of biocatalysis and provides a process for the production of ethylenediamine-N,N'-disuccinic acid, as well as a biocatalyst mixture for use in the production of ethylenediamine-N,N'-disuccinic acid.

The application belongs to the field of genetic engineering and bioengineering technology, and discloses a vanillin de novo synthesis method based on recombinant bacteria co-culture and application. The method designs a de novo biosynthesis pathway containing tyrosine deaminase, ferulic acid decarboxylase and other key enzymes, constructs an Escherichia coli co-culture system composed of an upstream recombinant strain producing ferulic acid from glucose and a downstream recombinant strain converting ferulic acid into vanillin, and after optimizing the inoculation ratio of the strains, the highest yield of 7.8 mg / L is obtained under specific fermentation conditions with glucose as the substrate. The method is environmentally friendly and efficient, establishes a microbial platform for industrial production of vanillin, and provides technical support for large-scale production of vanillin and its derivatives.

The present invention relates to guanidinoacetic acid, characterized in that it comprises less than 0.3 ppm of melamine and / or less than 600 ppm of dicyandiamide and / or cyanamide as impurity or secondary component, determined by LC-MS / MS, a process for its preparation, a feed additive comprising or consisting of the guanidinoacetic acid according to the present invention or of the guanidinoacetic acid obtained by the process according to the present invention.

This invention provides an L-homoserine-producing strain, its construction method, and its applications. The strain does not contain plasmids. E.coli W3110, as a chassis strain, had the relA and thrB genes knocked out, and heterologously expressed genes derived from... Clostridium acetobutylicum The gapC gene and the aspB gene derived from Bacillus subtilis were overexpressed, along with ppc, asd, and thrA. fbr ,rhtA,pntAB,spoT fbr Genes; the strain described above can effectively improve the L-homoserine synthesis efficiency by knocking out genes of the L-homoserine degradation pathway, enhancing the expression of key enzymes in the pathway, optimizing the L-homoserine transport system, increasing the reducing power NADPH content, and enhancing the ability to withstand amino acid starvation. It is genetically stable, requires no addition of resistance substances, has high acid production efficiency, and has good L-homoserine synthesis capacity.

The application discloses a high-yield resveratrol Yarrowia lipolytica engineering strain and construction and application thereof, and belongs to the technical field of synthetic biology and metabolic engineering. The Yarrowia lipolytica engineering strain provided by the application can realize high-yield resveratrol by using glucose as a substrate, and the yield of resveratrol reaches 3.157 g / L in shake flask fermentation, and the yield of resveratrol is increased to 30.7 g / L in 5L scale batch feeding fermentation, which is the highest value reported at present.