High yield L-leucine engineering bacterium and application thereof

A technology of Leucine and Corynebacterium glutamicum, applied in application, genetic engineering, bacteria, etc., can solve the problems of increased miscellaneous acids, long cycle, heavy workload, etc.
CN104480058AActive Publication Date: 2015-04-01FUJIAN NORMAL UNIV +1
2 Cites 17 Cited by

Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
FUJIAN NORMAL UNIV
Publication Date
2015-04-01

Smart Images

  • Figure 1
    Figure 1
  • Figure 2
    Figure 2
  • Figure 3
    Figure 3
Patent Text Reader

Abstract

The invention discloses a high yield L-leucine engineering bacterium and an application of the bacterium.The invention provides a method for preparing a recombinant bacterium. The method comprises the following step of importing an alpha-isopropylmolic acid synthetase mutant encoding gene into a target bacterium to form the recombinant bacterium, wherein an alpha-isopropylmolic acid synthetase mutant is enzyme obtained by mutating 494th arginine of alpha-isopropylmolic acid synthetase into histidine, 497th glycine into aspartic acid and 499th leucine into valine.The high yield L- leucine engineering bacterium, particularly corynebacterium glutamicum MD0032, has higher L-leucine yield than bacterial strains produced at home at present, has a unique identification label and is a L-leucine production strain with a high production and application value.
Need to check novelty before this filing date? Find Prior Art

Description

technical field

[0001] The invention relates to the field of biotechnology, in particular to a high-yielding L-leucine engineering bacterium and its application. Background technique

[0002] The molecular structure of L-leucine, L-isoleucine (L-isoleucine) and L-valine (L-valine) contains a methyl side chain, so it is called branched chain amino acid (branched chain amino acid). acids, BCAA). As one of the eight essential amino acids for the human body, L-leucine is a raw material for the synthesis of human hormones and enzymes. It has the effect of promoting protein synthesis and inhibiting decomposition, but it cannot be synthesized in the body. Therefore, L-leucine has a wide range of applications and commercial value in the fields of food, medicine, feed and sports health care, and with the continuous development of the function of L-leucine, its application fields are gradually expanding, and the market is also continuing rising.

[0003] At present, the production ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More