302 results about "Branched-chain amino acid" patented technology

The invention provides a nursing sow high-efficiency feed and a preparation method thereof. Raw materials such as corn, white sugar, wheat bran, fish meal, soya bean meal, puffed soybean, feather meal, blood globulin powder, distillers dried grains with soluble (DDGS), soybean oil, vitamine, trace elements, an ionic equilibrium agent, a physiological regulator and the like are adopted, and the nursing sow high-efficiency feed is prepared by performing smashing, mixing, tampering, pelletizing and other processing on the raw materials. In the invention, the newest theory of the existing animal nutrition and feeding is taken as a basis and production practice is combined, thus the defects of the prior art are overcome. The high-efficiency feed has the following advantages: the current cultivation situation in the southern part of China, market requirements and the newest sow nutrition theory are combined, and maximization of productive potential of a nursing sow is taken as an aim, especially amino acid balance is considered, so that branched chain amino acid nutrition is enriched, the sow antistress capability is improved, and the palatability of the feed is improved; and the ionic balance of the feed is considered, high-quality feed raw materials are selected, and strict scientific processing is carried out, thus the milk production performance of the nursing sow can be greatly improved, finally the weight of weaning litter of suckling pigs is improved, and the market requirement is met.

A composition includes a plurality of amino acids. The plurality of amino acids includes at least one essential amino acid and at least one non-essential amino acid. The plurality of amino acids also includes at least one branch-chain amino acid. The composition also includes a source of carbohydrates. The compositions also includes purified water. The plurality of amino acids comprises about 1 wt % of the composition. A composition includes a plurality of amino acids, sodium citrate, sodium chloride, potassium phosphate, flavoring, a source of carbohydrates, and purified water. In some embodiments of the composition, the plurality of amino acids includes alanine, arginine, aspartate, cystine, glutamine, glycine, histidine, isoleucine, leucine, lysine, methionine, phenylalanine, proline, serine, threonin, tryptophan, tyrosine, and valine.

A method of modulating plasma levels of branched chain amino acids and branched chain alpha-keto acids is disclosed, wherein an ammonia scavenger compound or a salt thereof, for example phenylbutyrate or an even numbered congener thereof or a salt thereof, is administered to an individual in need thereof. In various methods, a decrease in plasma levels of branched chain amino acids and branched chain alpha-keto acids is effected to treat individuals suffering from an inborn error in metabolism of amino acids, such as Maple Syrup Urine Disease, for example.

The invention provides methods for treating muscle loss in an individual. In one embodiment, the invention includes administering to an individual an effective amount of a branched chain amino acid (BCAA), a BCAA precursor, a BCAA metabolite, a BCAA-rich protein, a protein manipulated to enrich the BCAA content or any combination thereof. The invention further provides nutritional products for such administration, including orally-administrable nutritional products

The invention relates to a high-protein dietary fiber energy protein bar and a production method thereof. The protein bar comprises the following materials in parts by weight: 5-35 parts of soy protein, 0-31 parts of cocoa butter replacer chocolate, 3-29 parts of glucose syrup, 0-18 parts of oligomeric isomaltose, 3-8 parts of concentrated whey protein powder and 3-9 parts of plaint oil. According to the formula of the protein bar, the soy protein, the concentrated whey protein, the glucose syrup and the oligomeric isomaltose are used as main raw materials for simultaneously supplementing exercising people with protein and energy (carbohydrate). The added concentrated whey protein has nutrient substances such as high-quality alpha-lactalbumin, beta-lactoglobulin and branched chain amino acid, thus being beneficial to rapidly supplementing protein after movement and relieving fatigue; the protein, energy (carbohydrate) and functional nutrient substances (such as dietary fiber) are rapidly supplemented to the exercising people; the protein bar provided by the invention is easy to carry, convenient to eat and good in taste.

The invention provides a genetically engineered bacterium for high-yielding L-valine. A construction method of the genetically engineered bacterium comprises the steps that starting from an escherichia coli W3110, an acetolactate synthase gene alsS of a bacillus subtilis is integrated on a genome of the escherichia coli W3110 and subjected to high expression; an escherichia coli ppGpp 3'-pyrophosphoric acid hydrolytic enzyme mutant R290E/K292D gene spoT is integrated on the genome of the escherichia coli W3110 and subjected to high expression; genes of frdA, frdB, frdC and frdD of four subunits of a lactic dehydrogenase gene ldhA, a pyruvate formate lyase I gene pflB and fumaric reductase on the genome of the escherichia coli W3110 are knocked out; a branched chain amino acid transaminasegene ilvE of the escherichia coli is replaced with leucine dehydrogenase gene bcd of the bacillus subtilis; and an acetyl-hydroxyl acid isomerized reductase gene ilvC of the escherichia coli is replaced with an encoding gene of a mutant L67E/R68F/K75E. According to the genetically engineered bacterium for the high-yielding L-valine, an L-valine fermentation method is further modified. Double-phasedissolved oxygen control is adopted, and the L-valine yield and the saccharic acid conversion rate are improved.

An object of the present invention is to provide: a novel gene (polynucleotide) encoding an FAD-conjugated glucose dehydrogenase having excellent properties that it has excellent reactivity to glucose, excellent thermal stability, and excellent substrate-recognition performance and also has a low activity for maltose; a process for the production of the enzyme using a transformant cell transfected with the gene; and a method for the determination of glucose, a reagent composition for use in the determination of glucose, a biosensor for use in the determination of glucose and others, each characterized by using the enzyme obtained. The invention relates to a polynucleotide encoding an FAD-conjugated glucose dehydrogenase, comprising a polypeptide containing an amino acid sequence: X1-X2-X3-X4-X5-X6 (wherein X1 and X2 independently represent an aliphatic amino acid residue; X3 and X6 independently represent a branched amino acid residue; and X4 and X5 independently represent a heterocyclic amino acid residue or an aromatic amino acid residue); and others.

Methods useful for reducing or preventing non-alcoholic steatohepatitis or hepatic steatosis are provided herein. Such methods may comprise administering to a subject in need thereof a sirtuin pathway activator and/or PDE5 inhibitor alone or in combination with an amount of a branched amino acid in free amino acid form, or a metabolite thereof. Also provided herein are compositions and kits for practicing any of the methods described herein.

The invention discloses a corynebacteria expression system without depending on antibiotic being selection pressure, and belongs to the microbe gene engineering field. Alr knock out carrier and expression carrier used for plasmid anaplerosis are constructed to bring conveniences for lab research and utilization, and a fermentation process no longer needs antibiotic. Through test results, the expression system realizes thalline inner plasmid stability in fermentation without adding the antibiotic; when the expression system is used to construct overexpression vector of branched chain amino acid-valine synthesis path main gene ilvBNC, valine output can be obviously improved; the corynebacteria expression system has wide application value.

The present invention provides a fatigue-preventive (brain fatigue) agent or a fatigue-recovering agent for the central nervous system, which contains a branched-chain amino acid, such as L-valine, L-leucine and L-isoleucine, and/or a 2-aminobicyclo-[2,2,1]-heptane-2-carboxylic acid. In addition to the application as injection and transfusion, these can be prepared as solid-state modes to be taken, such as tablets, pellets and powder medicine by adding an appropriate vehicle such as starch and lactose thereto. Moreover, these can be prepared as various beverages such as so-called health drinks and sports drinks, or as food items for fatigue-recovering and the fatigue-prevention in the central nervous system.

The present invention provides for compositions, methods and kits for regulating energy metabolism. In one aspect, the invention provides for compositions that comprise a combination of (a) branched chain amino acids, such as leucine, and (b) vitamin B6, or any precursors or metabolites of (a) or (b). These combinations may be synergistic and/or effective for reducing weight or adipose volume. In another aspect, the invention provides for methods of regulating energy metabolism by the administration of one or more compositions comprising branched chain amino acids and vitamin B6. The invention also provides for kits comprising compositions of branched chain amino acids and vitamin B6 packaged in an oral dose form with usage instructions.

The invention relates to a recombinant DNA (deoxyribonucleic acid), strain and method for producing L-valine by fermentation. The recombinant DNA comprises DNA sequence for coding acetohydroxy acid synthetase free of feedback inhibition of three branched-chain amino acids to acetohydroxy acid synthetase, DNA sequence for coding dihydroxy reduction isomerase, and DNA sequence for coding branched-chain amino acid aminotransferase. The strain is corynebacterium or brevibacterium transformed by introducing the recombinant DNA. The method is implemented by culturing the strain to produce the L-valine. In the invention, the expression L-valine is used for producing the bacterium L-valine biosynthetic gene, so the yield of the L-valine can be greatly increased; and the fermentation model, especially the high-temperature short-time fermentation model, is constructed according to the optimum temperature of the L-valine biosynthetic enzyme, so that the metabolism intensity of the strain is enhanced, the activity of the L-valine biosynthetic enzyme is enhanced, and the yield of the L-valine and the conversion rate of the sugar acid can be further increased.

The invention provides an orally-administrable nutritional product comprising a dipeptide including a branched chain amino acid (BCAA). In one embodiment, the nutritional product comprises a dipeptide selected from at least one of the following: alanyl-leucine, alanyl-isoleucine, alanyl-valine, glycyl-leucine, glycyl-isoleucine, and glycyl-valine.

The present invention concerns an improved balance of the essential branched chain amino acids leucine, isoleucine and valine in infant formula.

The invention discloses an anti-fatigue energy composition which is prepared by the following raw materials in parts by weight: expanded black rice flour, oatmeal, coconut oil, corn syrup, soybean protein isolate powder, maltodextrin, carnitine, vine tea flavone extract, grape seed extract, quercetin extract, octacosanol, caffeine, branched chain amino acid, taurine, low fat cocoa and concentrated vanilla; the anti-fatigue energy composition is capable of rapidly supplementing sugar, fat, protein, electrolyte and other nutrients which are needed by the organism and promoting the nutrients to be absorbed, can be used for rapidly supplementing the consumed carbohydrate and the lost nutrients of human body caused by sweating excessively under the condition of exercises, especially medium and high intensity exercises and exercise training, and is beneficial for relieving central and peripheral fatigue; after the anti-fatigue energy composition is adopted, the antioxidant capacity and the aerobic metabolism level of the organism are improved, the oxygen utilization rate of cells can be improved, the moment and fatigue resistance of the organism can be better rapidly improved, and the sport injury is avoided.

A prophylactic or therapeutic composition for hemoglobinuria or myoglobinuria which may occur in humans or animals upon loading of exercise or stress or the like is provided.

According to the present invention, the prophylactic or therapeutic composition for hemoglobinuria or myoglobinuria which comprises a branched-chain amino acid such as valine, leucine or isoleucine or a salt thereof, a basic amino acid such as ornithine, arginine, lysine, histidine or citrulline or a salt thereof and glutamine or a salt thereof as active ingredients can be provided.

The invention discloses high-F-ratio leech oligopeptide and an enzymatic hydrolysis preparation method and application thereof. The preparation method comprises the following steps: (1) preliminarily cleaning dried leech bodies, removing impurities and carrying out high-speed crushing; (2) pre-soaking raw materials with alkali; (3) carrying out enzymatic hydrolysis by using double enzymes consisting of alkali protease AlcalaseAF 2.4L and papain; (4) statically adsorbing and removing aromatic amino acids by using active carbon powder; and (5) carrying out further separation and purification by using a gel filtration chromatographic column, collecting a separation product and then carrying out freeze drying so as to obtain leech oligopeptide powder with a molecular weight of less than 2000 Da and an F ratio (Fishcer ratio, i.e., a ratio of the mole content of branched-chain amino acids to the mole content of aromatic amino acids) of more than 20. According to the invention, preparation process is simple, two commercial enzymes consisting of alkali protease and papain are selected and used, industrial production can be easily realized, high yield is obtained, and the high-F-ratio leech oligopeptide has a certain anticoagulation effect and increases a novel source for high-F-ratio products.