1312 results about "Alanine" patented technology

The invention discloses a high-performance carbonyl reductase mutant and application thereof in synthesis of a series of chiral alcohols. The high-performance carbonyl reductase mutant is obtained by performing the following mutations on an amino acid sequence as shown in SEQ ID NO.2: the 69th alanine mutates into any one of lysine, leucine, aspartic acid or asparagine, and the 69th alanine mutates into any one of lysine, leucine, aspartic acid or asparagine; and / or the 100th glycine is mutated into any one of glutamic acid, serine, lysine or asparagine. Compared with a wild type enzyme, the thermal stability and the catalytic activity of the obtained mutant are remarkably improved, particularly, single point mutants Mut-A69N, Mut-G100E and Mut-G100N and a combined mutant Mut-A69N-G100N show excellent relative enzyme activity, thermal stability and substrate tolerance. The mutant has high catalytic activity and stability to various chiral alcohols, and the contradiction that the traditional carbonyl reductase is easy to inactivate at high temperature and the catalytic efficiency is difficult to achieve at the same time is solved. The invention provides an efficient and stable enzyme catalysis tool for green biological preparation of chiral drug intermediates.

The invention relates to the technical field of biological catalysis, and discloses an L379A mutant enzyme for preparing rebaudioside I. The enzyme is obtained by the following mutations generated by UGT76G1: leucine of the 379th amino acid sequence is mutated into alanine; the enzyme can be applied to RA in-vitro conversion preparation of RI with higher utilization value, the conversion rate is higher than 50%, and the enzyme activity is remarkably improved by 7 times compared with the original enzyme catalytic conversion enzyme activity. The UGT76G1 mutant disclosed by the invention has the advantages that (1) the blank is filled, and a special enzyme catalyst capable of realizing efficient in-vitro synthesis of rebaudioside I (RI) is provided for the first time; 2) efficiency jump: the catalytic conversion rate is greatly increased from original about 7% to more than 50% (increase gt; and 3) stability and reliability: in the cross-scale reaction of 10mL to 5L, the catalyst has stable catalytic performance, shows excellent industrial application potential, and is suitable for popularization and application.

The invention discloses an alanine aminotransferase detection device and method based on a liquid crystal microcavity array, and belongs to the field of optical sensing. The device comprises a functional liquid crystal microcavity array, an excitation optical fiber and a collection optical fiber, the microcavity array is composed of liquid crystal microcavities doped with fluorescent dye and stearic acid, and the multiple microcavities are independently distributed and serve as optical sensing units; the excitation optical fiber is used for synchronously exciting the microcavity to generate an echo wall mode, and the acquisition optical fiber acquires resonance spectrum signals of each channel in real time; the functionalized liquid crystal microcavity is converted into a bipolar configuration from a radial configuration in an enzymatic reaction, echo wall mode spectrum red shift is caused, spectrum shift time is in negative correlation with enzyme concentration, and dual-channel detection is realized by matching with conversion time of a polarized light pattern. According to the detection structure based on the liquid crystal microcavity array provided by the invention, high-flux, real-time and label-free rapid detection on the concentration of alanine aminotransferase can be realized.

The invention relates to the technical field of microorganisms, and particularly discloses a recombinant microorganism and application thereof in fermentation production of decarboxylated carnosine. Compared with an original strain, the recombinant microorganism disclosed by the invention has the advantages that the expression of the mdtK gene is enhanced; the starting strain is escherichia coli capable of producing decarboxylated carnosine. When the recombinant microorganism is used for fermentation production, the yield of the decarboxylated carnosine can be increased, L-histidine, histamine or beta-alanine and other precursors do not need to be added in the production process, and an effective novel method is provided for industrial production of the decarboxylated carnosine.

The invention provides a preparation method of 3-boron-L-phenylalanine or a derivative of 3-boron-L-phenylalanine. The preparation method provided by the invention comprises the step of preparing 3-boron-L-phenylalanine as shown in a formula (VI) or a derivative thereof by taking a compound as shown in a formula (III) as a raw material. The method has the advantages of mild reaction conditions, short route, high yield, few byproducts and low cost, and does not need an extremely low temperature condition process or complicated purification steps. # imgabs0 #

The invention relates to the technical field of biological catalysis, and discloses an M88A mutant enzyme for preparing rebaudioside I. The enzyme is obtained by the following mutations generated by UGT76G1: M88A: methionine of the 88th amino acid sequence of UGT76G1 is mutated into alanine; the key conversion rate of RI is greatly increased from 7% to 85% (increased by more than 12 times), and the utilization rate and economical efficiency of raw materials are remarkably improved; the method can be perfectly reproduced under any test scale (10mL, 100mL and 5L), the conversion rate is always anchored to be more than 85%, and incomparable stability and amplification potential are shown.

The application discloses a galactosyltransferase mutant and application thereof in preparation of flavonoid compounds, and belongs to the technical field of enzymology. The application provides a UDP-galactosyltransferase mutant, which is obtained by site-directed mutation of one amino acid in the amino acid sequence of wild-type galactosyltransferase VcUFGT into alanine (A). The effects of a series of mutants H82A, V139A, G141A, P186A, N245A, V282A and S307A are verified in the examples, the mutants can perform enzyme catalysis reaction with quercetin and UDP-galactoside as substrates, and generate flavonoid compound hyperoside, and the specific enzyme activity of the mutants is more than 1.5 times that of the wild type, thereby providing an effective application basis for biosynthesis of flavonoid compounds.

The present invention discloses a pectin methylesterase mutant ΔDdPemA, its encoding gene, preparation method, and application. The pectin methylesterase mutant ΔDdPemA is obtained by mutating the amino acid at position 200 in the center of the substrate binding pocket of the wild-type pectin methylesterase DdPemA from a high-molecular-weight, branched valine to a low-molecular-weight alanine, and by mutating the amino acid at position 339 at the substrate inlet and outlet of the substrate binding pocket from a high-molecular-weight, branched valine to a basic lysine. The amino acid sequence and nucleotide sequence of the wild-type pectin methylesterase DdPemA are SEQ ID NO. 1 and SEQ ID NO. 2, respectively. The mutant enzyme provided by the present invention has significantly improved enzyme activity under weakly acidic conditions, solving the problem of insufficient catalytic activity of the wild-type pectin methylesterase under weakly acidic conditions and creating favorable conditions for the application of the enzyme in the fields of food, textiles, and feed processing.

The invention provides a method for detecting free amino acids in camellia oleosa seed oil, and establishes a method for detecting the free amino acids in the camellia oleosa seed oil based on ultra-high performance liquid chromatography-tandem mass spectrometry (UPLC-MS / MS), which comprises the following steps: pre-treating various amino acids in the camellia oleosa seed oil by using a non-derivation method; the problems of complicated operation, impurity interference and the like caused by derivatization are avoided, the extraction solvent is environment-friendly and economical, the extraction efficiency is high, and the pretreatment time is greatly shortened; then selecting a proper chromatographic column and a mobile phase, separating a plurality of free amino acids under the condition of not introducing excessive derivatization agents and ion pair reagents, and controlling the collection time to be 12 minutes. The method for detecting the free amino acids in the camellia oleosa seed oil has the characteristics of no need of derivatization treatment, simplicity and convenience in operation, high detection efficiency and good sensitivity and accuracy, and can effectively separate and detect 14 free amino acids such as alanine, phenylalanine and aspartic acid in the camellia oleosa seed oil.

The invention discloses a polypeptide with a whitening effect and a preparation method and application thereof.The polypeptide is obtained from hydrolyzed protein of babylonia areolata, the amino acid sequence of the polypeptide is cysteine-arginine-proline-threonine-cysteine-serine-arginine-leucine-alanine-cysteine, and the amino acid sequence of the polypeptide is cysteine-arginine-proline-threonine-cysteine-serine-arginine-leucine-alanine. The compound can obviously inhibit the generation of melanin in cells and the activity of tyrosinase in vitro, has the characteristics of low toxicity, low irritation, good stability, safety and high efficiency, and can be applied to whitening cosmetics.

The invention relates to a high-carbon biological organic conditioner and application thereof in red soil peanut planting, the high-carbon biological organic conditioner comprises mineral powder, oyster shell powder, zeolite powder, potassium fulvic acid, decomposed cow dung, alanine and glycine according to a mass part ratio of 800: 200: 10: 50: 140: 0.7: 0.5, the content of organic matters in the high-carbon biological organic conditioner is not less than 45 wt.%, the content of humic acid is 20 wt.%, the content of potassium fulvic acid is 20 wt.%, and the content of potassium fulvic acid is 20 wt.%. And bacillus subtilis is contained. By applying the high-carbon biological organic conditioner, the soil quality of the red soil peanut planting area is rapidly improved, the peanut yield is remarkably increased, and an effective technical solution is provided for agricultural sustainable development of the red soil area.

The invention relates to the technical field of biological catalysis, and discloses an L204A mutant enzyme for preparing rebaudioside I. The enzyme is obtained by the following mutations generated by UGT76G1: L204A: leucine of the 204 amino acid sequence of UGT76G1 mutates into alanine; the high-efficiency in-vitro enzymatic preparation of the rebaudioside I (RI) is realized for the first time, the catalytic efficiency of the rebaudioside I (RI) is improved by more than 7 times (from about 7% to more than or equal to 50%) compared with that of an original enzyme, the high-efficiency performance can be stably maintained under different scales of 10mL to 5L, a solid foundation is laid for industrial application of the RI, and the preparation method is simple, controllable in process condition and suitable for popularization and application.

The invention relates to the field of plant molecular biology and forest genetic engineering, and particularly provides a PagRAP2.3 protein point mutant and application thereof in drought resistance of poplar. The mutant PagRAP2.3 MA is obtained by mutating methionine at the first site and cysteine at the second site of a wild type PagRAP2.3 protein into methionine and alanine. The invention further discloses a preparation method of the mutant PagRAP2.3. An expression vector containing the PagRAP2.3 MA gene is constructed, poplar 84K is transformed through an agrobacterium-mediated method, and a transgenic line with stable expression is obtained. Functional verification results show that overexpression of PagRAP2.3 MA can significantly increase the plant height of the poplar and promote plant growth, but the sensitivity to moisture is enhanced under drought stress. The mutant can be used for regulating and controlling the growth and development of forest trees and evaluating the drought resistance, and has a forestry breeding application prospect.

The invention relates to a TaCBL4 mediated TaSOS1 phosphorylation regulation and control site in wheat and an application of the TaCBL4 mediated TaSOS1 phosphorylation regulation and control site. The TaSOS1 phosphorylation regulation and control site is Ser978-Ser1126 / Ser1128 (S978-DSPS); the phosphorylation regulation and control site of the TaSOS1 is S978-DSPS, and serine at the site 978 of the TaSOS1-DAA (S1126AS1128A) is mutated into alanine. The TaSOS1 in the wheat is from a chromosome LOC778390, NC057800.1 (13146069..13157356) of the 3A of the common wheat, and the TaSOS1 in the wheat is from a chromosome NC057800.1 (13146069..13157356). The invention also relates to an application of the TaCBL4 mediated TaSOS1 phosphorylation regulation site Ser978-Ser126 / Ser128 (S978-DSPS) in the wheat as a molecular marker for detecting the salt tolerance of the wheat, and also relates to an application of the TaCBL4 mediated TaSOS1 phosphorylation regulation site. The site is applied to the aspects of wheat salt tolerance improvement and assisted breeding.

The disclosure relates to double halogenated phenylalanine derivatives, which can be radiolabeled with PET or SPECT radioisotopes, and their use in treating and / or imaging cancer.

The present application relates to a pyridoxal kinase mutant, a recombinant expression vector and a microbial cell and their applications, and belongs to the technical field of biological catalysis. In order to solve the problem of low product concentration in existing enzyme catalysis, a pyridoxal kinase mutant is provided, the amino acid sequence is selected from the amino acid sequence shown in SEQ ID NO. 1, the lysine at position 229 is mutated to alanine, phenylalanine, methionine, arginine, threonine, histidine, serine, tyrosine, valine, leucine, isoleucine, proline, asparagine, aspartic acid or glutamic acid; the pyridoxal kinase mutant is used for catalyzing pyridoxal to synthesize pyridoxal phosphate, and a recombinant expression vector and a microbial cell can be further formed. The present application has good enzyme activity, high product conversion rate, high concentration of pyridoxal phosphate obtained, and the concentration of the product catalyzed by the wild-type pyridoxal kinase to phosphorylate pyridoxal is obviously improved.

The invention relates to the technical field of concealed project acceptance, and discloses an alpine region bluegrass cold-resistant growth-promoting nutrient solution and an application method thereof.The nutrient solution is composed of main nutrient elements such as nitrogen, phosphorus and potassium and microelements such as iron, manganese, zinc, copper and boron, and amino acid substances such as glycine, alanine and proline are added; therefore, comprehensive and balanced nutrition support is provided. Through a scientific fertilization strategy, including three stages of seed treatment, seedling stage management and growth stage management, and in combination with a drip irrigation system and a foliage spraying technology, efficient utilization of nutrients and healthy growth of plants are ensured. According to the method, the growth speed and biomass of the bluegrass are remarkably increased, the soil structure is improved, the stress resistance of crops is enhanced, the nutrient utilization efficiency is improved, and finally the product quality and market value are improved; the comprehensive benefits are beneficial to promoting the sustainable development of agriculture in alpine regions.

The present disclosure relates to a protein-based article comprising a film having a thickness of about 0.1 μm to about 10 mm and comprising a crosslinked network of partially ordered polypeptides, wherein each partially ordered polypeptide independently comprises a plurality of disordered domains and a plurality of ordered domains, wherein each disordered domain independently comprises a PG or GP motif and each ordered domain independently comprises a polyalanine motif or a polyproline motif.

The invention belongs to the technical field of biological medicine, and particularly relates to application of phenylalanine in evaluating Kawasaki disease and treating effect thereof and application of a phenylalanine inhibitor in treating Kawasaki disease. According to serum non-targeted metabonomics analysis and verification of KD children and IVIG treated KD children, phenylalanine is highly expressed in KD children, and phenylalanine is remarkably reduced after IVIG treatment of KD and tends to healthy children. The phenylalanine can be used as a serological marker for evaluating the Kawasaki disease treatment effect and diagnosing IVIG second-line treatment, and is high in specificity and sensitivity. After the phenylalanine inhibitor is administered to a KD model mouse, the content of phenylalanine in serum is remarkably reduced and is consistent with the trend in serum of the KD mouse treated by IVIG, myrica tongues are remarkably relieved, arterial dilatation is slowed down, and expression of inflammatory factors is reduced. The phenylalanine inhibitor has the effects of treating Kawasaki disease, improving myrica rubra tongue and slowing down arterial dilatation.

An aqueous pharmaceutical composition including compound having the formula of tetragadolinium [4,10-bis(carboxylatomethyl)-7-{-3,6,12,15-tetraoxo-16-[4,7,10-tris(carboxylatomethyl)-1,4,7,10-tetraazacyclododecan-1-yl]-9,9-bis({[({2-[4,7,10-tris(carboxylatomethyl)-1,4,7,10-tetraazacyclododecan-1-yl]propanoyl}amino)acetyl]amino}methyl)-4,7,11,14-tetraazaheptadecan-2-yl}-1,4,7,10-tetraazacyclododecan-1-yl]acetate wherein the stereochemistry at the chiral carbon of the four alanine substituents is selected from the group consisting of RRRR, SSSS, RSSS, RRSS, and RRRS stereoisomers, and racemic and diastereomeric mixtures of any thereof, or a tautomer, a hydrate, a solvate, or a salt thereof, or a mixture of same is described. The compounds may be used as an MRI contrast imaging agent.

The invention discloses a construction method of a cyanobacteria-corynebacterium glutamicum artificial photosynthetic mixed bacteria system. The construction method comprises the following steps: step S1, amplifying by taking plasmid pUC-tho as a template to obtain a theophylline inducible promoter Ptho; step S2, carrying out amplification by taking a Synechocystis sp. PCC 6803 genome as a template so as to obtain a strong promoter Pcpc560, and carrying out amplification on the strong promoter Pcpc560 so as to obtain a strong promoter Pcpc560; step S3, carrying out amplification by taking the plasmid pUC-PAL as a template to obtain an arabidopsis thaliana phenylalanine lyase gene pal; step S4, carrying out amplification by taking the plasmid pUC-cscB as a template to obtain an escherichia coli W sucrose transporter gene cscB; step S5, carrying out amplification by taking the integrated plasmid Pcp3031 as a template, so as to obtain a terminator Trbcl; and step S6, fusing the strong promoter Pcpc560, the arabidopsis phenylalanine lyase gene pal and the terminator Trbcl, and respectively connecting with an expression vector pSI-SPE to obtain the expression vector containing the gene pal. According to the invention, a'cyanobacteria-corynebacterium glutamicum 'artificial photosynthetic mixed bacteria system is constructed by simulating a mutualistic symbiotic relationship ubiquitous in nature, and negative carbon biosynthesis of cis-muconic acid is realized.

The invention discloses a preparation method of a hydrophobic modified copper-based monatomic catalyst. The preparation method comprises the following steps: S1, dissolving Cu (NO3) 2 and L-alanine in deionized water; dissolving trimesic acid in an ethanol solution; s2, mixing the two solutions, and stirring at regular time at normal temperature to obtain a Cu-BTC precursor; s3, grinding and uniformly mixing the Cu-BTC precursor and dicyandiamide, and then putting the mixture into a tubular furnace for high-temperature pyrolysis treatment to obtain Cu-N-C-Pre powder; s4, the Cu-N-C-Pre powder is subjected to acid leaching impurity removal treatment in an acid solution, and the copper-based monatomic catalyst is obtained; and S5, dissolving the Cu-N-C catalyst and triacetylene benzene in an organic solvent, adding pyridine, uniformly stirring, and carrying out a hydrothermal reaction to obtain the hydrophobic modified copper-based monatomic catalyst. Hydrophobic modification is performed on the surface of the Cu-N-C monatomic catalyst, so that the hydrophobicity of the surface of the catalyst is enhanced, the catalytic activity of the catalyst in a reaction for preparing phenol by activating a benzene C-H bond by taking H2O2 as an oxidizing agent is improved, and the yield of phenol is increased.

In the first aspect, the present disclosure provides a Lactoferrin polypeptide fragment, where the Lactoferrin polypeptide fragment has an amino acid sequence shown in SEQ ID NO: 1. In the second aspect, the present disclosure provides a preparation method of the Lactoferrin polypeptide fragment, including: protecting amino acids using a 9-fluorenylmethoxycarbonyl (Fmoc) protecting group, coupling the amino acids with a P-hydroxymethylphenoxymethyl polyethylene resin (HMP resin) in an order of cysteine, alanine, leucine, cysteine, glutamate, threonine, asparagine, aspartate, asparagine, phenylalanine, leucine, leucine, asparagine, and lysine, and separating the HMP resin to obtain a target Lactoferrin polypeptide fragment. In the third aspect, the present disclosure further provides an anti-Lactoferrin antibody prepared using the Lactoferrin polypeptide fragment, and use of the Lactoferrin polypeptide fragment in preparation of an anti-Lactoferrin flow cytometric antibody and in preparation of a Lactoferrin detection product.

The invention relates to the technical field of microorganisms, and particularly discloses a PhPepD mutant and application of the PhPepD mutant in fermentation production of L-carnosine. According to the invention, a new PhPepD mutant is screened by means of construction of a mutation library from PepD of different sources and error-prone PCR, site-directed saturation mutation and the like, the amino acid sequence of the PhPepD mutant is shown as SEQ ID NO: 18, and compared with wild type PhPepD protein, the PhPepD mutant has N158D / I176F / E393Q mutation. By using the mutant, a microorganism with increased L-carnosine yield can be obtained, and in recombinant microorganisms capable of producing carnosine without adding beta-alanine, the mutant also shows an effect of increasing the L-carnosine yield, and a new effective method is provided for industrial large-scale production of L-carnosine.

The invention discloses an amino acid composition for treating alopecia and application of the amino acid composition in preparation of a medicine for treating alopecia, and belongs to the field of medicine configuration products, the amino acid composition is composed of a targeting core intervention component and an auxiliary strengthening synergistic component, the targeting core intervention component is prepared from 30-50 parts of arginine, 50-155 parts of lysine and 50-120 parts of glutamic acid, and the auxiliary strengthening synergistic component is prepared from an auxiliary strengthening synergistic component and an auxiliary strengthening synergistic component. 10 to 40 parts of cysteine and 20 to 60 parts of leucine; the auxiliary strengthening synergistic component is prepared from 15 to 45 parts of tyrosine, 15 to 40 parts of glycine, 10 to 40 parts of glutamine, 20 to 35 parts of serine, 5 to 25 parts of alanine, 2 to 20 parts of aspartic acid and 15 to 35 parts of phenylalanine; the composition can be used as an active ingredient to be prepared into different dosage forms such as a pigmentum, a spray, an ointment and a liniment, is applied to prevention of alopecia and promotion of hair growth, and has a remarkable treatment effect.

The invention relates to a synthetic method of a refined dimethenamid intermediate, which comprises the following steps: adding methanol and L-alanine into a reaction flask, starting to heat, dropwise adding an esterification reagent at a certain temperature while keeping the temperature, reacting at the same temperature while keeping the temperature after dropwise adding is finished, and after the reaction is finished, carrying out post-treatment to obtain a crude product of (S)-2-amino methyl propionate; adding a solvent and a first reducing agent into the reaction flask, dropwise adding the (S)-2-methyl aminopropionate crude product and a second reducing agent at low temperature, and after dropwise adding, carrying out heat preservation reaction for a period of time at the same temperature; and raising the reaction temperature, carrying out high-temperature heat-preservation reaction for a period of time, and carrying out post-treatment to obtain the refined dimethenamid intermediate (S)-(+)-1-methoxy-2-propylamine. When the method is used for preparation, the product can be obtained only by esterifying and reducing the raw materials, the steps are simple, the raw materials are cheap and easy to obtain, and the yield is greater than 90%.

According to the scheme, the invention provides the recombinant macrolide enzyme as well as the preparation method and the application thereof, the amino acid sequence of the recombinant macrolide enzyme is as shown in SEQ ID NO.1, and the recombinant macrolide enzyme is derived from an EreC esterase family of enterobacter hormaechei and is obtained through site-specific mutagenesis; the mutation sites are as follows: glutamic acid at the 44th site is mutated into asparagine, tyrosine at the 55th site is mutated into proline, proline at the 76th site is mutated into arginine, phenylalanine at the 153rd site is mutated into alanine, and serine at the 219th site is mutated into glutamic acid. The method is expected to remove macrolide antibiotic pollution, and has good economic benefits and practical values.

The invention discloses an application of beta-alanine in preparation of a medicine for treating pulmonary arterial hypertension. In-vivo and in-vitro pharmacological experiments prove that beta-alanine can inhibit lactic acid-mediated histone milk acylation modification and hypoxia-induced phenotypic transformation of PASMCs (Polyaspartic Sulfonate Molecules); the hemodynamics and pulmonary artery vascular remodeling of the PAH mouse are improved. The beta-alanine can inhibit phenotypic transformation of PASMCs and reverse pulmonary artery vascular remodeling which is a key pathological change in the pulmonary hypertension generation process, has huge potential in treatment of pulmonary hypertension, and has good application prospects and high clinical application value.

The invention discloses application of copper-amino acid nano enzyme in preparation of anti-inflammatory drugs, and relates to the technical field of biomedical new materials, amino acids comprise glycine, arginine, histidine, threonine, phenylalanine and cysteine; the ratio of the amino acid to the copper ions is 1: (0.5-5), and the synthesis temperature of the copper-cysteine nano enzyme is 25-125 DEG C. The copper-amino acid nano-enzyme library established by the invention has efficient hydroxyl free radical, superoxide free radical and hydrogen peroxide scavenging activity; wherein the copper-cysteine nano-enzyme shows the highest enzymatic activity, and shows low toxicity and good biocompatibility in both the cell level and the animal level; meanwhile, the copper-cysteine nano-enzyme also shows anti-inflammatory activity and anti-oxidative stress activity, can remarkably improve cell inflammation and body inflammation, relieves and treats dextran sodium sulfate induced mouse ulcerative colitis, and can be further applied to preparation of drugs for treating inflammatory bowel diseases.

The present invention relates to binding proteins which bind alanine aminotransferase (ALT). The present invention also relates to bivalent, trivalent, quadrivalent or multivalent binding proteins that bind ALT. The present invention also relates to nucleic acids, vectors and host cells for producing such binding proteins, methods for producing such binding proteins, kits comprising such binding proteins and methods and uses of such binding proteins.