2140 results about "Alanine" patented technology

The invention belongs to the technical field of biochemical engineering, and provides a method for increasing L-carnosine yield of escherichia coli by increasing PRPP supply, and the yield and yield of L-carnosine are remarkably increased by up-regulating expression of a prs gene, increasing PRPP content in cells and promoting synthesis of L-histidine. Experiments show that the yield of the L-carnosine in different recombinant strains is increased by 55.56%-140%, and L-histidine and beta-alanine do not need to be added in the production process.

The invention relates to L-alanine, a preparation method and a fermentation device, and relates to the technical field of L-alanine. The culture medium contains a carbon source, a nitrogen source, a phosphorus source and trace elements; the L-alanine is synthesized by the microorganisms by controlling fermentation conditions and time; the invention further relates to an L-alanine preparation method, an L-alanine preparation device is adopted, the L-alanine preparation device comprises a fermentation tank and a stirrer for mixing a plurality of raw materials of L-alanine, and the stirrer is further provided with a treatment part for treating floating foam, oily substances and impurities generated in the stirring process. The processing part can collect floating foam on the surface of the mixed solution and can also collect impurities and oily substances in the mixed solution, and meanwhile an opening and closing door on the processing part can be opened and closed, so that the impurities difficult to capture in the mixed solution fluctuation state are collected in a unified mode; and the removal rate of floating foam and impurities is greatly improved.

The invention belongs to the technical field of protein engineering, and discloses a Brazilian sweet protein mutant and application thereof. According to the invention, lysine is used for replacing aspartic acid at the 29th site of Brazilian sweet protein, and / or arginine is used for replacing glutamic acid at the 36th site, and / or alanine is used for replacing aspartic acid at the 50th site, amino acid mutation at the sites is carried out to construct a Brazilian sweet protein mutant, and the Brazilian sweet protein mutant is subjected to heterologous expression in pichia pastoris GS115, so that the Brazilian sweet protein mutant is obtained. And carrying out sweetness performance evaluation on the expressed Brazilian sweet protein mutant. The sweetness property of the double mutant Asp29Lys-Asp50Ala protein is improved by 240% compared with the sweetness property of the Brazilian sweet protein before mutation.

The invention relates to the technical field of daily chemicals, in particular to a hair repairing agent and application thereof. The shampoo is prepared from the following raw materials: a suspending agent, a surfactant, a hair conditioner, a solvent, a cleaning agent, a functional repairing agent, a preservative, an aromatic, a thickening agent and a humectant. Fibroin is degummed through bifidus yeast by adopting a biological fermentation technology to obtain a protein active matter with lower molecular weight, a fibroin derivative is rich in a large amount of amino acid, glycine, alanine and serine account for about 85% of the total composition, the fibroin derivative has very strong moisturizing performance, the extract can rapidly permeate into hair cores, and the effect of moisturizing the hair cores is achieved. The water bonding capability of hair is enhanced, naturally existing free amino acids and lost polypeptides are supplemented, the fiber structure of the hair is recombined, the strength, toughness and glossiness of the hair are enhanced, and branching and embrittlement are prevented.

The invention discloses a high-performance carbonyl reductase mutant and application thereof in synthesis of a series of chiral alcohols. The high-performance carbonyl reductase mutant is obtained by performing the following mutations on an amino acid sequence as shown in SEQ ID NO.2: the 69th alanine mutates into any one of lysine, leucine, aspartic acid or asparagine, and the 69th alanine mutates into any one of lysine, leucine, aspartic acid or asparagine; and / or the 100th glycine is mutated into any one of glutamic acid, serine, lysine or asparagine. Compared with a wild type enzyme, the thermal stability and the catalytic activity of the obtained mutant are remarkably improved, particularly, single point mutants Mut-A69N, Mut-G100E and Mut-G100N and a combined mutant Mut-A69N-G100N show excellent relative enzyme activity, thermal stability and substrate tolerance. The mutant has high catalytic activity and stability to various chiral alcohols, and the contradiction that the traditional carbonyl reductase is easy to inactivate at high temperature and the catalytic efficiency is difficult to achieve at the same time is solved. The invention provides an efficient and stable enzyme catalysis tool for green biological preparation of chiral drug intermediates.

The invention relates to an effective amount of novel acyl phenylalanine derivative as shown in a general formula (I), a preparation method thereof and application of a pharmaceutical composition containing the derivative as a medicine, and compared with the prior art, the novel acyl phenylalanine derivative has a wider application prospect. # imgabs0 #

VH domain, in which: (i) the amino acid residue at position 1 12 is one of K or Q; and / or (ii) the amino acid residue at position 89 is T; and / or (iii) the amino acid residue at position 89 is L and the amino acid residue at position 1 10 is one of K or Q; and (iv) in each of cases (i) to (hi), the amino acid at position 1 1 is preferably V; and in which said VH domain contains a C-terminal extension (X)n, in which n is 1 to 10, preferably 1 to 5, such as 1, 2, 3, 4 or 5 (and preferably 1 or 2, such as 1); and each X is an (preferably naturally occurring) amino acid residue that is independently chosen, and preferably independently chosen from the group consisting of alanine (A), glycine (G), valine (V), leucine (L) or isoleucine (I).

The present invention relates to Alanine analogues of the formula (I) (I) where the variables are as defined in the claims and the description, and to the use of said Alanine analogues for controlling unwanted vegetation, and to methods of applying the Alanine analogues.

The invention relates to the technical field of biological catalysis, and discloses an L379A mutant enzyme for preparing rebaudioside I. The enzyme is obtained by the following mutations generated by UGT76G1: leucine of the 379th amino acid sequence is mutated into alanine; the enzyme can be applied to RA in-vitro conversion preparation of RI with higher utilization value, the conversion rate is higher than 50%, and the enzyme activity is remarkably improved by 7 times compared with the original enzyme catalytic conversion enzyme activity. The UGT76G1 mutant disclosed by the invention has the advantages that (1) the blank is filled, and a special enzyme catalyst capable of realizing efficient in-vitro synthesis of rebaudioside I (RI) is provided for the first time; 2) efficiency jump: the catalytic conversion rate is greatly increased from original about 7% to more than 50% (increase gt; and 3) stability and reliability: in the cross-scale reaction of 10mL to 5L, the catalyst has stable catalytic performance, shows excellent industrial application potential, and is suitable for popularization and application.

The invention belongs to the field of biological medicine, and particularly relates to a respiratory syncytial virus F protein mutant and application thereof. The respiratory syncytial virus F protein mutant is characterized in that at least one pair of amino acid residues in an F1 subunit and / or an F2 subunit of F protein polypeptide are substituted by cysteine, and / or an F protein signal peptide part is substituted by a human immunoglobulin light chain signal peptide, and / or alanine A at the 107 site of a multi-base sequence is mutated into arginine R, lysine K at the 134 site is mutated into arginine R, and the amino acid residues in the F1 subunit and / or the F2 subunit of the F protein polypeptide are mutated into arginine. According to the invention, more neutralizing antibody epitopes can be exposed, the stable pre-fusion conformation is maintained, effective neutralizing antibody reaction and antibody binding reaction to the RSV subtypes A and B can be ensured, and the vaccine is suitable for various vaccine forms taking the RSV F protein as the antigen.

The invention relates to the field of polypeptide compositions, in particular to a polypeptide metal chelate and application thereof in the field of cosmetics, and the polypeptide metal chelate is formed by chelating a polypeptide compound and a metal ion compound. The structure of the polypeptide compound is [gamma-Glu] n-AA, n is greater than or equal to 1, and AA is selected from glycine, alanine, valine, leucine, isoleucine, methionine (methionine), proline, tryptophan, serine, tyrosine, cysteine, phenylalanine, asparagine, glutamine, threonine, asparaginic acid, glutamic acid, lysine, arginine or histidine; the polypeptide chelated zinc prepared by the invention has remarkable oil control and anti-inflammatory effects, and can be compounded with other raw materials to form cosmetics with oil control and acne removal effects.

The invention discloses an ACE (Angiotensin Converting Enzyme) inhibition and DPP-IV (Dipeptidyl Peptidase-IV) inhibition bifunctional peptide as well as a design method and application thereof. The amino acid sequence of the bifunctional peptide fragment is Trp-Ala-Leu-Val-Ala-Pro or Trp-Pro-Leu-Val-Ala-Pro. According to the design method, hydrophobic amino acid replacement is carried out on most N-terminal first amino acid according to the characteristics of the DPP-IV inhibitory peptide, and N-terminal second amino acid is kept as proline Pro or alanine Ala. The difunctional peptide fragment obtained through the DPP-IV inhibitory activity optimization strategy has the effects of assisting in lowering blood pressure and lowering blood sugar at the same time and can be used for preparing functional food or health food with the functions of assisting in lowering blood pressure and lowering blood sugar, and the optimization strategy can be applied to improving the blood sugar lowering effect of the peptide fragment and has good market application prospects.

The invention discloses an alanine aminotransferase detection device and method based on a liquid crystal microcavity array, and belongs to the field of optical sensing. The device comprises a functional liquid crystal microcavity array, an excitation optical fiber and a collection optical fiber, the microcavity array is composed of liquid crystal microcavities doped with fluorescent dye and stearic acid, and the multiple microcavities are independently distributed and serve as optical sensing units; the excitation optical fiber is used for synchronously exciting the microcavity to generate an echo wall mode, and the acquisition optical fiber acquires resonance spectrum signals of each channel in real time; the functionalized liquid crystal microcavity is converted into a bipolar configuration from a radial configuration in an enzymatic reaction, echo wall mode spectrum red shift is caused, spectrum shift time is in negative correlation with enzyme concentration, and dual-channel detection is realized by matching with conversion time of a polarized light pattern. According to the detection structure based on the liquid crystal microcavity array provided by the invention, high-flux, real-time and label-free rapid detection on the concentration of alanine aminotransferase can be realized.

Provided are novel dipeptide repeat (DPR) specific antibodies (e.g., human-derived antibodies) as well as synthetic variants and biotechnological derivatives thereof, capable of binding C9orf72 poly-glycine-alanine DPRs, as well as methods and uses related thereto. The antibody of the present invention can be used in pharmaceutical and diagnostic compositions for DPR protein-targeted immunotherapy and diagnostics.

The invention provides a method for producing L-phenylalanine, 4-hydroxymandelic acid and / or a PHA material through a one-carbon resource and application of the L-phenylalanine, the 4-hydroxymandelic acid and / or the PHA material. According to the method, a halomonas strain which cannot be directly produced by using a one-carbon resource originally is modified, and a carbon dioxide fixed gene is added, so that recombinant bacteria capable of producing L-phenylalanine and 4-hydroxymandelic acid are constructed, and co-production of extracellular L-phenylalanine, 4-hydroxymandelic acid and other high-added-value compounds and intracellular polyhydroxyalkanoate (PHA) is realized. According to the invention, a more economic, sustainable and high-benefit platform for producing L-phenylalanine, 4-hydroxymandelic acid and PHA is established, and the method plays an important role in further reduction of production cost and improvement of sustainable development.

The invention relates to the technical field of microorganisms, and particularly discloses a recombinant microorganism and application thereof in fermentation production of decarboxylated carnosine. Compared with an original strain, the recombinant microorganism disclosed by the invention has the advantages that the expression of the mdtK gene is enhanced; the starting strain is escherichia coli capable of producing decarboxylated carnosine. When the recombinant microorganism is used for fermentation production, the yield of the decarboxylated carnosine can be increased, L-histidine, histamine or beta-alanine and other precursors do not need to be added in the production process, and an effective novel method is provided for industrial production of the decarboxylated carnosine.

The invention provides a preparation method of 3-boron-L-phenylalanine or a derivative of 3-boron-L-phenylalanine. The preparation method provided by the invention comprises the step of preparing 3-boron-L-phenylalanine as shown in a formula (VI) or a derivative thereof by taking a compound as shown in a formula (III) as a raw material. The method has the advantages of mild reaction conditions, short route, high yield, few byproducts and low cost, and does not need an extremely low temperature condition process or complicated purification steps. # imgabs0 #

The invention discloses a high-activity TET dioxygenase mutant as well as a construction method and application thereof. The TET dioxygenase mutant is obtained from an amino acid sequence of wild type TET dioxygenase as shown in SEQ ID NO.1 according to any one of the following mutation modes: a, the 150th proline is mutated into alanine; and b, proline at the 150th site is mutated into alanine, and isoleucine at the 235th site is mutated into methionine. The TET dioxygenase mutant provided by the invention can be stabilized in an escherichia coli system, the purification effect is good, the preparation difficulty is reduced, and the activity is remarkably improved compared with that of a wild type. The methylation gene transformation effect is far better than that of a traditional bisulfite transformation method, and the method has a good application prospect.

An artificial synthesis method for malonyl-CoA and use thereof are provided. By means of heterologous expression of an aminotransferase and a malonyl-CoA reductase, an artificial synthesis pathway for synthesizing malonyl-CoA by using β-alanine (β-ala) as a precursor is constructed as follows: firstly, under catalysis of a transaminase, β-ala transfers amino groups to α-ketonic acid (such as pyruvic acid, oxaloacetic acid, or α-ketoglutaric acid, etc.), to form an intermediate product 3-oxopropanoate and a corresponding amino acid; the 3-oxopropanoate generates malonyl-CoA under the action of the malonyl-CoA reductase. This pathway addresses the defects of the natural malonyl-CoA synthesis pathway, such as low carbon utilization, consumption of energy substance ATP, release of greenhouse gas CO2, and strict regulation of pathway enzymes, a pyruvate dehydrogenase (PDH) and an acetyl-CoA carboxylase (ACC), thereby achieving high yielding of products using malonyl-CoA as a precursor, including flaviolin, octanoic acid, phloroglucinol, pentadecaheptaene, natamycin, and spinosad.

The invention discloses a delayed coagulation type polycarboxylate superplasticizer and a preparation method thereof, and relates to the field of concrete admixtures. The retarding type polycarboxylic acid water reducing agent is prepared from 45 to 55 parts of unsaturated polyether monomer, 15 to 20 parts of quaternary ammonium salt cationic monomer, 10 to 15 parts of unsaturated sulfonic acid or unsaturated sulfonate monomer, 5.5 to 7.5 parts of unsaturated phosphate monomer, 4.8 to 6.2 parts of unsaturated hydroxy fatty acid ester monomer, 100 to 120 parts of acrylic acid, 1 to 2 parts of chain transfer agent, 1.5 to 2 parts of oxidizing agent, 0.75 to 1 part of reducing agent and 500 parts of water. The structural general formula of the unsaturated polyether monomer is R1CH2O (CH2CH2O) nR2, R1 is an alkenyl group, and R2 is any one of a phenyl group and a phenylalanine alkyl ester group. The delayed coagulation type polycarboxylate superplasticizer can effectively improve the delayed coagulation effect of a concrete mixture with the fine aggregate mud content larger than 1.5% in a high-temperature environment, and constructability is ensured.

The invention belongs to the technical field of skin care product raw materials, and discloses an oligopeptide compound, a preparation method thereof, a beautifying composition and application. The oligopeptide compound has a structural formula of # imgabs0. The oligopeptide compound provided by the invention contains peptide fragments of antioxidant amino acids such as tyrosine (Tyr), beta-alanine (beta-Ala), cysteine (Cys) and proline (Pro), and the amino acids can directly participate in removal of free radicals or form a stable peptide structure to enhance the antioxidant effect; therefore, the oligopeptide compound with anti-oxidation and physiological repair effects is obtained. Meanwhile, a mild Fmoc route is adopted in the preparation method, the condensation reaction time of each step is short, the production period is greatly shortened, raw materials are convenient to obtain, the process is stable, the quality is controllable, the product yield and purity are high, and large-scale production can be achieved.

The invention discloses application of synbiotics to prevention and control of plutella xylostella pests. Probiotics in the synbiotics are a pseudomonas strain PPF1, the pseudomonas strain PPF1 is classified and named as Pseudomonas putida, and the prebiotic composition is ornithine, alanine, valine, serine, leucine and cycloleucine. The invention further discloses a preparation method of the prebiotic composition. Wherein the pseudomonas can improve the resistance of Chinese cabbage to plutella xylostella. According to the prebiotic composition, the resistance of Chinese cabbage to plutella xylostella is improved, meanwhile, the growth of the pseudomonas strain PPF1 is remarkably promoted, and the colonization ability of the pseudomonas strain PPF1 is improved. The synbiotics are applied into soil by a root irrigation method, so that the feeding area of the cabbage by the plutella xylostella can be remarkably reduced, and the pest of the plutella xylostella in the cabbage can be effectively prevented and controlled.

A boron carrying agent, a preparation method therefor, and a pharmaceutical formulation. The boron carrying agent contains compound 1, N-4-carboxyphenylboronic acid amide-threonine-phenylalanine-phenylalanine-tyrosine-glycine-glycine-serine-arginine-glycine-lysine-arginine-aspartic acid-aspartic acid-phenylalanine-lysine-threonine-glutamic acid-glutamic acid-tyrosine-cysteine, having a molecular formula of C114H158BN30O35S1 and a molecular weight of 2552.4, and further contains compound 2, N-4-carboxyphenylboronic acid amide-arginine-lysine-lysine-lysine-arginine-arginine-glutamine-arginine-arginine-arginine, and / or compound 3, N-4-carboxyphenylboronic acid amide-methionine-alanine-serine-methionine-threonine-glycine-glycine-glutamine-glutamine-methionine-glycine. Compounds 1-3 can serve as a targeted medicament for boron neutron capture therapy used for treating a cerebral glioma and a head and neck tumor.

The invention discloses a C1, 25-bit dihydroxylase mutant of vitamin D3 as well as a genetically engineered bacterium and application thereof, and belongs to the technical field of enzyme engineering. The preparation method comprises the following steps: mutating 81-site glutamic acid of vitamin D3 dihydroxylase of bacillus megatherium H-1 into alanine to obtain a vitamin D3 dihydroxylase mutant; pMA5 is used as an expression plasmid to realize heterologous expression of the vitamin D3 dihydroxylase mutant in the bacillus subtilis; when the engineering bacteria disclosed by the invention are applied to calcitriol synthesis, the mutant has the effects of increasing the concentration accumulation amount of a product and shortening the reaction time consumption, and the production requirements of shortening the time cost in industrial production are met.

The invention relates to the technical field of biological catalysis, and discloses an M88A mutant enzyme for preparing rebaudioside I. The enzyme is obtained by the following mutations generated by UGT76G1: M88A: methionine of the 88th amino acid sequence of UGT76G1 is mutated into alanine; the key conversion rate of RI is greatly increased from 7% to 85% (increased by more than 12 times), and the utilization rate and economical efficiency of raw materials are remarkably improved; the method can be perfectly reproduced under any test scale (10mL, 100mL and 5L), the conversion rate is always anchored to be more than 85%, and incomparable stability and amplification potential are shown.

The application discloses a galactosyltransferase mutant and application thereof in preparation of flavonoid compounds, and belongs to the technical field of enzymology. The application provides a UDP-galactosyltransferase mutant, which is obtained by site-directed mutation of one amino acid in the amino acid sequence of wild-type galactosyltransferase VcUFGT into alanine (A). The effects of a series of mutants H82A, V139A, G141A, P186A, N245A, V282A and S307A are verified in the examples, the mutants can perform enzyme catalysis reaction with quercetin and UDP-galactoside as substrates, and generate flavonoid compound hyperoside, and the specific enzyme activity of the mutants is more than 1.5 times that of the wild type, thereby providing an effective application basis for biosynthesis of flavonoid compounds.

The invention discloses a serum-free culture medium for in-vitro proliferation of poultry muscle stem cells and application of the serum-free culture medium, and belongs to the technical field of cell engineering, a basic serum-free culture medium is DMEM-F12, a complete serum culture medium is a mixture of DMEM-F12 added with 10% of fetal calf serum and 1% of mycillin, and the volume ratio of a culture medium supplement to the basic culture medium is 1: 1000; the serum-free culture medium contains a supplement; the supplement comprises the following compositions: insulin, transferrin, fibroblast growth factor 2, epidermal growth factor, catalase, ethanolamine, vitamin B12, linoleic acid, linolenic acid, retinol acetate, tocopherol, L-alanine, L-proline, sodium selenite, sodium pyruvate, reduced glutathione, berberine hydrochloride and analogues thereof.

The invention relates to a recombinant thallus, a construction method of the recombinant thallus and application of the recombinant thallus in production of xanthoxylin WGX-50, and relates to the technical field of bioengineering. A recombinant plasmid containing a phenylalanine ammonialyase gene PAL with a nucleotide sequence as shown in SEQ ID NO.2 and a cinnamic acid coenzyme A ligase gene CNL with a nucleotide sequence as shown in SEQ ID NO.1 and a recombinant plasmid containing a tyramine N-hydroxycinnamoyl transferase gene THT with a nucleotide sequence as shown in SEQ ID NO.3 are introduced into the recombinant plasmid. A phenylalanine ammonialyase gene PAL, a cinnamic acid coenzyme A ligase gene CNL and a tyramine N-hydroxycinnamoyl transferase gene THT are overexpressed in escherichia coli, so that a biological synthesis path of xanthoxylin WGX-50 is formed by taking 3, 4-dioxomethyl phenylethylamine as a substrate for the first time, a foundation is laid for large-scale industrial production, and the method has a wide application prospect. The method has important economic value and social benefit.

The present invention discloses a pectin methylesterase mutant ΔDdPemA, its encoding gene, preparation method, and application. The pectin methylesterase mutant ΔDdPemA is obtained by mutating the amino acid at position 200 in the center of the substrate binding pocket of the wild-type pectin methylesterase DdPemA from a high-molecular-weight, branched valine to a low-molecular-weight alanine, and by mutating the amino acid at position 339 at the substrate inlet and outlet of the substrate binding pocket from a high-molecular-weight, branched valine to a basic lysine. The amino acid sequence and nucleotide sequence of the wild-type pectin methylesterase DdPemA are SEQ ID NO. 1 and SEQ ID NO. 2, respectively. The mutant enzyme provided by the present invention has significantly improved enzyme activity under weakly acidic conditions, solving the problem of insufficient catalytic activity of the wild-type pectin methylesterase under weakly acidic conditions and creating favorable conditions for the application of the enzyme in the fields of food, textiles, and feed processing.

The present invention discloses a complete medium for culturing DF-1 cells, including DMEM basal medium 13.7g / L, insulin, non-essential amino acids, transferrin, glutathione, ascorbyl phosphate, sodium selenite, ethanolamine, hydrocortisone, and newborn calf serum. Wherein non-essential amino acids include glycine, alanine, proline, serine, and asparagine. A complete medium for culturing DF-1 cells provided by the present invention can well promote the proliferation of DF-1 cells, can achieve the same proliferation effect as high-concentration fetal bovine serum, and is suitable for the adherent culture of DF-1 cells; using low-concentration newborn calf serum, it is possible to greatly avoid the batch differences, unstable culture results, cytotoxicity, and high risk of carrying pathogens brought by the culture medium with high-concentration serum, narrow application range, expensive fetal bovine serum, and high culture cost; the components are simple, the culture medium preparation process is short in time consumption, the culture efficiency is high, the repeatability is good, and it is suitable for batch production.