86 results about "Galactoside" patented technology

The invention discloses a chromogenic culture medium for detecting gram-negative bacteria in bovine mastitis and a test plate thereof, the culture medium comprises a nutritional ingredient mixture, a composite inhibitor, a composite chromogenic enzyme substrate and cold water gel, the compound chromogenic enzyme substrate is red tetrazole, 5-bromo-6-chloro-3-indole-beta-D-galactoside, and 5-bromo-4-chloro-3-indole-beta-D-glucopyranoside, and the compound chromogenic enzyme substrate is a compound chromogenic enzyme substrate, and the compound chromogenic enzyme substrate is 5-bromo-6-chloro-3-indole-beta-D-glucopyranoside. The chromogenic culture medium and the test board prepared from the chromogenic culture medium can effectively inhibit the growth of non-gram negative bacteria, have the characteristics of strong specificity, high sensitivity, short detection period, easy observation and low detection cost, and have huge development potential and wide application prospects in microbiological detection.

The present invention belongs to the field of biotechnology, and specifically relates to a GmSGT2 gene related to plant height and branch development, and a mutant thereof and the use thereof. A Glycine max mutant having fewer branches and decreased plant height is obtained by means of EMS mutagenesis, and a target gene thereof that is located by means of a map-based cloning technique is a GmSGT2 gene of Hedou 12. It is found through searching that the gene encodes soyasapogenol B glucuronide galactosyltransferase, and can galactosylate soyasapogenol B monoglucuronide, thereby affecting the plant height of Glycine max and reducing the branches of Glycine max. Therefore, the GmSGT2 mutant can be used for cultivating dwarf high-yield Glycine max varieties, and is of great significance in the breeding of ideal plant types of Glycine max and research on important agronomic traits of plants. The mutant has broad application prospects and great research value with regard to understanding the plant height and branch regulation mechanism of Glycine max and improving the process of Glycine max breeding; and can provide an excellent germplasm resource stock for regulating the close planting and high-yield breeding of Glycine max.

The application discloses a galactosyltransferase mutant and application thereof in preparation of flavonoid compounds, and belongs to the technical field of enzymology. The application provides a UDP-galactosyltransferase mutant, which is obtained by site-directed mutation of one amino acid in the amino acid sequence of wild-type galactosyltransferase VcUFGT into alanine (A). The effects of a series of mutants H82A, V139A, G141A, P186A, N245A, V282A and S307A are verified in the examples, the mutants can perform enzyme catalysis reaction with quercetin and UDP-galactoside as substrates, and generate flavonoid compound hyperoside, and the specific enzyme activity of the mutants is more than 1.5 times that of the wild type, thereby providing an effective application basis for biosynthesis of flavonoid compounds.

The invention provides a genetically engineered bacterium for producing lactose-N-neotetraose as well as a construction method and application of the genetically engineered bacterium. The genetically engineered bacterium meets the following conditions: (1) exogenous expression of an MFS transport protein, beta-1, 3-N-acetylglucosamine transferase and beta-1, 4-galactosyl transferase is carried out; and (2) over-expression of a galactoside osmose enzyme and a UDP-galactose-4-epimerase is carried out. According to the genetically engineered bacterium disclosed by the invention, the MFS transporter, the beta-1, 3-N-acetylglucosamine transferase and the beta-1, 4-galactosyl transferase are exogenously introduced, and the expression quantity of galactosidase and UDP-galactose-4-epimerase in a genome is enhanced, so that the residual quantity of LNT II in an LNnT synthesis route can be effectively reduced, and the fermentation yield of the LNnT is increased.

The application discloses a kind of cydia pomella larva's food lure composition and application thereof.It includes the following weight parts of component: isoquercitrin 0.02~0.07 parts, phlorizin 0.6~0.9 parts, cyanidin-3-O-galactoside 0.01~0.02 parts, delphinidin-3-O-galactose 0.05~0.12 parts.The food lure component provided in the application has low dosage and low price, can improve the behavior regulation effect of lure core on larvae, reduce the production cost, and is suitable for popularization and promotion.

The application discloses efficient expression of Aspergillus oryzae beta-galactoside enzyme mutant and application thereof in dairy products, and belongs to the technical fields of enzyme engineering and dairy product processing, and particularly relates to efficient expression of Aspergillus oryzae beta-galactoside enzyme mutant and application thereof in dairy products.The mutant protein can be as follows: A1) a protein with an amino acid sequence of SEQ ID No.2; A2) a fusion protein with the same function obtained by connecting a label to the N terminal and / or C terminal of A1).The mutant protein is subjected to high-density fermentation in a 5L fermentation tank, and the enzyme activity of the fermentation liquor can reach 4628U / mL, and the recovery rate of the mutant enzyme is increased by 1.7 times.Compared with the wild type, the optimal pH of the mutant is increased from 4.5 to 5.5, the optimal temperature is decreased from 60 DEG C to 50 DEG C, the lactose hydrolysis efficiency is increased by 11%, and the mutant is more suitable for the production process of low / zero lactose dairy products.

The present invention relates to a D-galactopyranose compound of formula (1)whereinthe pyranose ring is α-D-galactopyranose, and these compounds are high affinity galectin-1 and / or galectin 3 inhibitors for use in treatment of inflammation; fibrosis; scarring; keloid formation; aberrant scar formation; surgical adhesions; septic shock; cancer; metastasising cancers; autoimmune diseases, metabolic disorders; heart disease; heart failure; pathological angiogenesis; eye diseases; atherosclerosis; metabolic diseases; diabetes type I; diabetes type II; insulin resistance; Diastolic heart failure; asthma; liver disorders.

The present invention belongs to the field of microbial culture and identification technology, and provides a Candida chromogenic culture medium and its preparation method and application. The present invention utilizes a specific concentration of chlorinated nitro blue tetrazolium, 5-bromo-4-chloro-3-indolyl phosphate p-toluidine salt, and 5-bromo-6-chloro-3-indole-β-D-galactoside as the main color-developing component in the Candida chromogenic culture medium, and further utilizes the Candida chromogenic culture medium of the present invention to cultivate different types of Candida for a period of time, and can display different colors according to different types of Candida, wherein Candida albicans can display obvious red, which is different from the colors of other types of Candida, and the red colony is not only conspicuous, but also easier to identify than light green or green colonies, and can be well distinguished from the colors of other colonies, so that identification personnel can directly observe and identify Candida albicans with the naked eye.

The application provides a galactoside agglutinin 10 (Gal10) crystal and a preparation method and prepared antibody IgY and a pharmaceutical preparation thereof, and belongs to the technical field of medicines. The application uses the galactoside agglutinin 10 crystal as an antigen to prepare an egg yolk neutralizing antibody IgY capable of dissolving the Gal10 crystal, has the effects of improving rhinitis or asthma of a patient, preventing and treating rhinitis and asthma, and is safe, has no side effects, has a long-lasting curative effect, and has no drug resistance, thereby providing a safe, simple, hygienic, effective, economic and personalized treatment mode for prevention and treatment of rhinitis and asthma, and having a high market value.

The invention relates to a preparation method and application of erythritol galactoside. The method specifically comprises the following steps: constructing a reaction system which takes o-nitrobenzene-beta-D-galactopyranoside as a glycosyl donor, erythritol as a receptor and recombinant beta-galactosidase Bga7 as a catalytic enzyme, so as to prepare the erythritol-beta-galactoside. Wherein the amino acid sequence of the beta-galactosidase Bga7 is as shown in SEQ ID No.2, and the nucleotide sequence of the beta-galactosidase Bga7 is as shown in SEQ ID No.1. The invention also provides an application of the erythritol-beta-galactoside in preparation of daily chemical products or medical preparations for resisting streptococcus mutans infection. The invention provides a novel beta-galactosidase Bga7 gene of a glycosidase family 2, and a novel tool enzyme is provided for synthesis of galactoside products. The erythritol-beta-galactoside prepared by the invention has good effects of preventing streptococcus mutans infection and caries, and can be used for preparing daily chemical products or medical preparations for preventing streptococcus mutans infection.

The invention provides a colonic microbiota responsive polymer characterized in that it is prepared by the polymerization of a response monomer and a linking monomer, wherein the response monomer is a cellobiose with a β-(1,4)-glucoside bond or / and a lactose with a β-galactoside bond; the linking monomer is a monomer that reacts with the hydroxyl group of the response monomer to form an ester or ether directly or by a crosslinking agent. The invention also provides a method for preparation of a colonic microbiota responsive polymer, a degradable film based on the polymer and a preparation method thereof. By means of the characteristics that β-(1,4)-glucoside bond provided by cellobiose or / and β-galactoside bond provided by lactose monomer unit in the polymer can specifically respond to the high abundance of microbiotas (β-glucosidase or / and β-galactosidase) in the colon to specifically degrade in the colon, a colon-targeted delivery system for diagnosis and treatment of colon diseases is constructed.

The invention discloses a liver targeting porous starch-luteolin compound and a preparation method and application thereof, and belongs to the technical field of drug delivery, corn porous starch is used as a carrier, and the compound with the liver targeting function is prepared through OSA hydrophobic modification, luteolin loading and epsilon-polylysine-galactoside targeting modification. The drug loading capacity and liver targeting property of luteolin are remarkably improved through triple modification, the production process is simple, the cost is low, and excellent blood sugar reducing and liver protecting effects are shown in diabetes liver injury treatment.

The present invention relates to a compound of formula (I) for use in a method for treatment of a cancer having a higher expression of galectin-1 compared to normal tissue.

The invention discloses a method for improving yield of anthocyanin extracted by an enzymic method, according to the method, phospholipase is added and used to act on a cell membrane structure, so that the extraction rate of the anthocyanin and the extraction rate of galactoside in the anthocyanin are obviously improved, and the extraction effect of the anthocyanin is greatly improved through a three-stage enzymolysis system. The total enzymolysis extraction time is within 4 h, and compared with 6-7 h of a traditional enzymatic extraction process, the production period is greatly shortened.

The application belongs to the technical field of microorganisms, and particularly relates to a Bifidobacterium breve RRJK1316 capable of promoting digestion and application thereof. Bifidobacterium breve The Bifidobacterium breve is named as Bifidobacterium breve (RRJK1316), is preserved in the Guangdong Microbial Culture Collection Center, and has a preservation number of GDMCC No. 64764. The strain has strong acid and bile salt resistance, can effectively resist the influence of gastric juice, and can maintain high activity after passing through the digestive tract. The strain has strong digestion promotion function, and the digestion promotion function is better than that of domperidone 75.0 mu g / mL when the concentration of live bacteria is 6.00*10 8 CFU / mL. Meanwhile, the strain has the function of relieving lactose intolerance, and the beta-galactoside enzyme activity reaches 0.793 U / mL.

The present invention relates to a method for producing α-sialyl-β-D-galactoside saccharides, particularly α-sialyl-(2→3)-β-D-galactoside saccharides and α-sialyl-(2→6)-β-D-galactoside saccharides, from a β-D-galactoside saccharide, a sialic acid donor, and an enzyme with β-galactoside α-sialyltransferase activity. The enzymes with β-galactoside α-sialyltransferase activity used herein do not exhibit catalytic activity towards the hydrolysis of cytidine 5'-monophospho-N-acetyl-neuraminic acid to cytidine and N-acetyl-neuraminic acid. The method can be performed in vitro and in vivo using a genetically engineered cell comprising a nucleic acid encoding said enzyme. Further, said process may be adapted to produce the sialic acid donor CMP-Neu5Ac from low-cost substrates N-acetyl-D-glucosamine (GlcNAc), pyruvate, a cytidine phosphate (CMP, CDP or CTP) and polyphosphate in a single reaction mixture with a set of optionally immobilized or optionally co-immobilized enzymes comprising N-acylglucoamine 2-epimerase (AGE), an N-acetylneuraminate lyase (NAL), an N-acylneuraminate cytidylyltransferase (CSS), optional a uridine kinase (UDK), a uridine monophosphate kinase and a polyphosphate kinase 3 (PPK3).

The invention belongs to the technical field of biological medicine, and particularly relates to a diglycerol linolenate galactoside compound in pepper as well as a preparation method and application of the diglycerol linolenate galactoside compound. The preparation method comprises the following steps: (1) taking red peppers to prepare pretreated red peppers; (2) adding the pretreated red peppers into an ethanol solution, performing ultrasonic extraction, and collecting first filtrate and first filter residues; (3) adding an ethanol solution into the first filter residue, carrying out ultrasonic extraction, combining the first filtrate and the second filtrate, and concentrating to obtain a crude extract; (4) separating the crude extract by adopting high-speed counter-current chromatography to obtain a primary purified product; and (5) further separating and purifying the primary purified product by adopting high performance liquid chromatography, and identifying to obtain the diglycerol linolenate galactoside compound. The purity of the diglycerol linolenate galactoside compound obtained by the preparation method is as high as 99.3%, and the diglycerol linolenate galactoside compound has a remarkable scavenging effect on DPPH free radicals, shows good antioxidant activity and has a wide application prospect.

The application discloses application of ZmGOLS2 genes and ZmRAFS genes in improving pollen vitality of corn under heat shock and drought conditions, and belongs to the technical field of genetic engineering. The application improves the pollen vitality of corn, promotes the germination of pollen under heat shock and drought stress conditions, and reduces the empty stalk rate of corn under drought stress conditions by simultaneously improving the expression levels of a corn myo-inositol galactoside synthetase coding gene and a corn raffinose synthetase coding gene.

The application provides a genetically engineered bacterium for producing lactose-N-neotetraose, a construction method and application thereof. The genetically engineered bacterium satisfies the following conditions: (1) exogenous expression of MFS transporter protein, beta-1, 3-N-acetylglucosamine transferase and beta-1, 4-galactosyltransferase; (2) overexpression of galactoside permease and UDP-galactose-4-epimerase; and (3) no expression or weakened expression of setA transporter protein. The applicant of the application finds through research that exogenous introduction of MFS transporter protein, beta-1, 3-N-acetylglucosamine transferase and beta-1, 4-galactosyltransferase in the genetically engineered bacterium, enhancement of the expression amount of galactoside permease and UDP-galactose-4-epimerase in the genome, and reduction of the expression amount of setA transporter protein can effectively reduce the residual amount of LNT II in the LNnT synthesis pathway and improve the fermentation yield of LNnT.

The application belongs to the technical field of biology and specifically relates to a method for synthesizing D-tagatose through multi-enzyme cascade catalysis, which uses lactose as a substrate and utilizes beta-galactoside enzyme, L-arabinose isomerase, glucose isomerase, fructokinase, tagatose-1,6-diphosphate aldolase, polyphosphate kinase and phosphatase for synchronous cascade catalysis to synthesize D-tagatose. The method improves the conversion rate of D-tagatose by continuously converting the intermediate product D-glucose into D-tagatose, provides a new method for synthesizing D-tagatose from lactose, and provides certain theoretical basis and technical support for realizing the industrialized production of high-value-added D-tagatose.

The application discloses a beta-galactoside enzyme mutant and application thereof. The application takes wild-type beta-galactoside enzyme Bgal1-3 as a parent to provide a mutant with improved thermal stability, enzyme activity and / or oligogalactose production capacity compared with the parent. Meanwhile, the application takes the mutant M9 of Bgal1-3 as a parent to provide a mutant with improved hydrolysis activity compared with the parent. The beta-galactoside enzyme mutant can be used for converting lactose to produce low / zero-lactose or oligogalactose-rich dairy products, and is beneficial to the development of low / zero-lactose or oligogalactose-rich dairy product related industry.

This invention provides the application of glucose-responsive nanohydrogels in the preparation of drugs for treating skin injuries. The method includes the following steps: mixing hyaluronic acid with a phenylboronic acid compound, adding 3-aminophenylboronic acid, 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride and N-hydroxysuccinimide, and performing a grafting reaction to obtain boric acid-modified hyaluronic acid; adding myricetin-3-O-galactoside, phospholipids, and cholesterol to an organic solvent, followed by thin-film hydration and ultrasonic treatment to obtain nanoliposomes; mixing the boric acid-modified hyaluronic acid with a polyvinyl alcohol solution, then adding the nanoliposome solution, and finally mixing to obtain the resulting nanohydrogel. The glucose-responsive nanohydrogel of this invention achieves adaptive drug release in the high glucose environment of diabetic wounds and regulates local metabolic homeostasis of the wound, especially the lysine degradation pathway in amino acid metabolism.

The present invention relates to a high affinity galectin-3 inhibitor alpha-D-galactopyranose derivative of formula (I), a pharmaceutical salt or solvate thereof, for use in treatment of inflammation.

The invention belongs to the field of food, and relates to a new application of myricetin-3-O-galactoside. The invention provides an application of myricetin-3-O-galactoside in preparation of a food for improving urinary tract infection. The invention also provides a food for improving urinary tract infection, and the food comprises myricetin-3-O-galactoside. The myricetin-3-O-galactoside is proved to have the effect of inhibiting the adhesion activity of urinary tract pathogenic escherichia coli for the first time, and the myricetin-3-O-galactoside can be used as an adhesion inhibiting active agent for urinary tract infection pathogen urinary tract pathogenic escherichia coli and can be used for preparing food for effectively improving urinary tract infection.

The invention relates to the technical field of biology, in particular to a method for synthesizing mucoprotein type O-glycan Core 6 through an enzyme method. Beta 1-3-galactosidic bonds of mucoprotein type O-glycan Core 2 are broken through catalysis of beta-galactosidase, galactose is removed, and the mucoprotein type O-glycan Core 6 is obtained, compared with a chemical method for synthesizing the mucoprotein type O-glycan Core 6, synthesis procedures are greatly reduced, input of a large amount of manpower, material resources and financial resources is avoided, the cost is low, the yield is high, and the selectivity is good; and a foundation is laid for synthesis of a series of O-glycans based on a mucoprotein type O-glycan Core 6 structure.

The application discloses a kind of similar bundle-like arabino-galactoside protein coding gene GhFLA2 In the application of regulating cotton seed vigor, the application obtains several overexpression materials, RNA interference materials and gene knockout materials by transgenic technology GhFLA2 It is found that overexpression GhFLA2 Can promote radicle elongation during seed germination and cotyledon unfolding after germination, GhFLA2 After the expression amount of the application is reduced or knocked out, radicle elongation and cotyledon development are inhibited.The application provides a new gene target for cotton seed vigor improvement.

The invention discloses a single-molecule fluorescence immunoassay kit for detecting dry plasma spot GFAP protein and a preparation method, and belongs to the technical field of immunodetection. The kit provided by the invention comprises an anti-GFAP mouse monoclonal antibody coated magnetic bead reagent, a biotinylated anti-GFAP mouse monoclonal antibody reagent, a streptavidin-beta-galactosidase reagent, a resorufin beta-D-galactoside reagent, a dry plasma spot eluent, a GFAP sample diluent, a GFAP calibration product, a GFAP quality control product and dry plasma spot filter paper. The dry plasma spot eluent contains PEG (Polyethylene Glycol) 6000 with the concentration of 15g / L to 25g / L, NaCl with the concentration of 15g / L to 20g / L, HEPES with the concentration of 11g / L to 12g / L, Triton X-100 with the concentration of 10g / L to 12g / L, EDTA (Ethylene Diamine Tetraacetic Acid) with the concentration of 4.5 mmol / L to 5.5 mmol / L, EGTA (Ethylene Glycol Terephthalate) with the concentration of 1.5 g / L to 2.0 g / L and BSA (Bovine Serum Albumin) with the concentration of 1.2 g / L to 1.8 g / L, and the pH value is adjusted to 7.3 to 7.5 by NaOH; aiming at the bottleneck problem of trace GFAP protein detection in a dried plasma spot sample, the detection sensitivity, accuracy and filter paper applicability are improved by optimizing an eluent formula, diluent components and a detection system.

The invention relates to the technical field of fruit tree cultivation, and particularly discloses a cultivation method for improving cold resistance of apple plants, which comprises the following steps: after apples grow to a dormancy period, spraying 80-120mg / L of inositol galactoside solution on the whole tree for 3-4 times every 10-11 days. According to the method provided by the invention, the cold resistance of the apple plants is remarkably improved.

The invention provides a construction method of the UPLC specific chromatogram of sword bean and preparation thereof, which comprises the following steps: A) dissolving a test raw material with a solvent, and extracting to obtain a to-be-detected solution; b) determining the liquid to be detected by adopting a high performance liquid chromatography to obtain the UPLC characteristic chromatogram of the sword bean and the preparation thereof; chromatographic conditions of the high performance liquid chromatography are as follows: a chromatographic column is a C18 column; a mobile phase A is an acetonitrile solution, a mobile phase B is a 0.1% phosphoric acid aqueous solution, and gradient elution is carried out. According to the method, a high performance liquid chromatography is adopted, an acetonitrile-0. 1% phosphoric acid solution is selected as a mobile phase for gradient elution, quercetin-3-O-glucose-7-O-rhamnoside, myricetin-3-O-galactoside, lysimachin, rutin and isoquercitrin are taken as reference substances, and the UPLC characteristic spectrum method of the sword beans and the preparation thereof is established. And more scientific technical means are provided for controlling the medicinal quality of the sword beans and the preparation thereof.

The application discloses a composition for alleviating the irritation of hair dyeing and a preparation method and application thereof, and the composition contains, in percentage by mass, 0.1-50% of a hook sand plant extract, 0.01-10% of a gelidium esculentum extract and 0.1-60% of a sargassum muticum extract. The hook sand plant extract, the gelidium esculentum extract and the sargassum muticum extract are compounded, and it is found that the obtained composition is rich in various proteins, sugars, vitamins, organic acids, mineral salts and trace elements, and the unique galactoside sulfate in the composition forms a firm protective film through ion-ion interaction between the amino groups of the skin and hair keratin and the amino groups of the galactoside sulfate, thereby alleviating the irritation of external chemicals, and the hydrogen bond between the hydroxyl groups and water has a good moisturizing effect and enhances the barrier function. The composition has the effect of alleviating the irritation of a hair dye to the scalp skin and hair during the hair dyeing process, and is helpful to dyeing, so that the hair is more colored.