521 results about "Insulin" patented technology

The invention relates to the technical field of microorganisms, in particular to a culture medium and a method for culturing Ackermania muciniphila for activating Amuc-1260 by using lambda-carrageenan as a carbon source, according to the culture method, the lambda-carrageenan is used for replacing a traditional carbon source, thallus growth and proliferation can be remarkably promoted, the effect is optimal under the condition of 2.00 g / L, and the culture cost is low. Compared with the conventional carbon source (glucose), the carbon source is improved by 92.91%. Further research finds that the lambda-carrageenan can induce the Akk bacteria to up-regulate the expression of glycoside hydrolase such as Amuc-1260 and enhance the activity of pathways such as glycosaminoglycan degradation, amino acid metabolism and insulin signals, so that the metabolic adaptability and energy utilization efficiency of the bacteria are improved. Meanwhile, the Akk bacteria secrete more functional short-chain fatty acids such as acetic acid, propionic acid and the like under the condition, and have the effects of regulating intestinal health and maintaining metabolic homeostasis. The invention provides a new theoretical basis and technical support for large-scale culture of Akk bacteria and development of a metabiotic product.

The invention discloses a culture medium and application thereof, and a method for in-vitro separation culture and adipogenic differentiation of precursor adipocytes in goat muscle, and relates to the technical field of cell culture, the culture medium comprises the following components: 8-12 [mu] g / mL of insulin, 8-12% by volume of fetal calf serum, 95-105 U / mL of penicillin, 95-105 [mu] g / mL of streptomycin and the like. On the basis of the prior art, a proper amount of liquiritin and pilocarpine are mainly added into a culture medium, and synergistic cooperation of the liquiritin and pilocarpine is found, so that the adipogenic differentiation capacity of cells such as mesenchymal stem cells and precursor adipocytes is remarkably improved, and the application range is wide. On the basis, the invention also provides a complete set of perfect in-vitro isolated culture and adipogenic differentiation method of the goat intramuscular precursor adipocytes, and a method and a way are provided for a series of researches developed through the goat primary intramuscular precursor adipocytes in the future.

Medication delivery methods, devices and systems are provided, the medication delivery device includes user interface elements, control circuitry, and a memory. The method includes receiving analyte measurement data of a user, presenting information related to the analyte measurement data to the user, and determining an intended dose of medication based on at least one of the analyte measurement data, optionally, therapeutic relevant conditions, therapy parameters, or a calculated insulin-on-board (IOB) value. When the user is queried to indicate that the user is ready for the delivery without presenting the intended dose on any of the one or more user interface elements of the medication delivery device, the intended dose is automatically delivered after receiving the user's indication.

The invention relates to the technical field of insulin pumps, in particular to a simple needle assisting module for an insulin pump, which comprises a bottom shell, and a fluid pushing module, a percutaneous insertion module and a reciprocating driving module which are arranged in the bottom shell, a mounting groove is formed in the bottom shell, the reciprocating driving module comprises a torsion spring, a guide block and a driving block, and the torsion spring has pre-torsion amount; one end of the torsion spring extends outwards and forms a connecting arm, a first limiting groove and a second limiting groove are formed in the installation groove, a strip-shaped groove is formed in the guide block, the driving block comprises a first sliding block used for fixing a needle body and a second sliding block used for fixing a sleeve, the guide block is arranged above the torsion spring, and the connecting arm of the torsion spring is movably arranged in the strip-shaped groove of the guide block. And in the process that the torsional spring releases the pre-torsion amount, the connecting arm drives the guide block to move in the mounting groove in the insertion or retraction direction. The insulin pump achieves needle assisting operation through cooperation of the extension arm at the end of the torsional spring and the guide block, and is simple in structure, convenient to install, good in stability and low in cost.

The invention provides an application of streptococcus salivarius in fatty liver, in particular to an application of streptococcus salivarius in preparation of a product, and the product is used for at least one of the following applications: (a) losing weight or reducing body fat; (b) improving the glucose tolerance of the patient; (c) increasing the sensitivity of the patient to insulin; (d) treating, relieving and preventing liver injury; (e) treating, relieving and preventing non-alcoholic steatohepatitis; (f) fat deposition caused by high-fat and high-cholesterol diet is reduced; (g) reducing blood fat; and / or (h) improving the level of at least one of the following indexes in the liver of the mammal: total cholesterol, triglyceride, low density lipoprotein-cholesterol and free fatty acid.

The invention belongs to the technical field of cell biology, and particularly discloses a culture medium and a culture method of fish primary liver cells. The culture medium of the fish primary liver cells comprises the following components: 90% of an M199 culture medium, 10% of fetal calf serum, 100 U / mL of penicillin, 0.1 mg / mL of streptomycin, 5 ng / mL of EGF, 10 [mu] U / ml of insulin and 10 mM of dexamethasone. Based on the culture medium, an efficient and stable fish liver cell culture method is developed by combining a standardized anesthesia and disinfection process, a precise dissection and liver sampling method, an optimized tissue digestion and cell separation scheme, an efficient cell purification and adherent culture system, a stable passage technology and the like; a standardized hepatocyte line establishment operation process is defined, and the problems that species binding exists in an existing fish liver cell culture method, and a standardized culture process suitable for multiple fish fingerlings is difficult to form are solved.

The invention provides isolated Ackermansiella muciniphila, a composition containing the same and an anti-aging and / or anti-oxidation application of the Ackermansiella muciniphila. The Akkermansia muciniphila has the advantages that the average life and the longest life of the Akkermansia muciniphila in a nematode model can be prolonged, the movement activity, the swallowing function and the reproductive capacity of the Akkermansia muciniphila can be improved, the expression of insulin / IGF-1 pathway genes (daf-16, daf-2 and age-1), the expression of antioxidant genes (gst-4 and sod-3) and the expression of longevity related genes (sir-2.1) of the Akkermansia muciniphila can be up-regulated, and the antioxidant capacity and the heat stress tolerance of the Akkermansia muciniphila can be enhanced. It is shown that the Akkermansia muciniphila provided by the invention can be used for preparing or developing the anti-oxidation and anti-aging products, and the anti-oxidation and anti-aging products can be prepared from the Akkermansia muciniphila. The invention provides an efficient and safe solution for anti-aging and / or anti-oxidation.

The invention relates to the technical field of clinical test data acquisition and intelligent quality control, in particular to a clinical test data real-time acquisition and quality control management platform, which is characterized in that an operation starting time recording unit acquires a subject disinfection operation starting timestamp, an operation sequence delay calculation unit calculates actual operation delay based on standard consumed time, and a quality control unit calculates the actual operation delay based on the standard consumed time. The transmission delay processing unit separates transmission and operation delay through double timestamps, the actual collection time determining unit subtracts pure transmission delay time data and operation delay time data from the timestamps uploaded by the device through a reverse time restoration algorithm to obtain actual blood collection completion time, and the time window dynamic judging unit judges whether the time window dynamic judging unit is in real blood collection completion time or not through a dynamic offset boundary. The real time is compared with a preset time window to judge qualification, the physiological index correction unit corrects blood glucose and insulin values in real time through a layered framework and a correction rule base, and the dynamic association logic verification unit adjusts association rules and verifies index logic in combination with a three-dimensional decision matrix, so that support is provided for clinical tests.

The invention discloses a gallbladder malignant tumor protein diagnosis biomarker combination, a kit and application. The protein marker provided by the invention comprises CA1 and / or IGFBP6 (insulin-like growth factor binding protein 6). According to the invention, an LC-MS / MS mass spectrometry method is adopted, mass spectrometry is carried out on a large number of clinical plasma samples, and by calculating log2 difference multiples of corresponding protein molecule contents in the plasma of a gallbladder cancer patient and the plasma of a normal person, it is determined that two protein molecules (CA1 protein and IGFBP6 protein) have good inspection benefits; the kit can be used as a marker for early diagnosis of gallbladder cancer; therefore, the invention provides a non-invasive screening means based on plasma, and the non-invasive screening means is used for predicting the cholecystic malignant tumor and distinguishing the cholecystic carcinoma crowd from the non-cancer crowd. And materials are convenient to take, the blood sample amount is small, and the sensitivity is high. The invention can fill the blank that no protein marker combination with high diagnostic efficiency exists at present for the cholecystic malignant tumor, and has great clinical significance.

1. Name of the design product: storage bag. 2. Use of the design product: The design product is used for storing blood glucose meters, insulin and other items needed by diabetes patients. 3. Design points of the design product: in shape. 4. Picture or photo best indicating the design points: perspective view.

The invention relates to the technical field of medical instruments, and discloses a small insulin pump with an insulin depletion reminding function, which comprises a shell, a medicine storage tube is inserted into a bottom cavity of the shell, a piston I is inserted into one end of the medicine storage tube, a push rod I is inserted into one end of the piston I, and a motor is mounted at the top of the cavity. A motor is arranged in the shell, a lead screw is connected to the output end of the motor, a vibration ringing mechanism is arranged on the top of the motor, and a battery bin is arranged on the top in the shell. By arranging the vibration ringing mechanism, a daytime pure vibration insulin depletion reminding mode and a night sound vibration composite insulin depletion reminding mode can be flexibly switched according to use scenes; in addition, the reminding intensity is dynamically changed along with the stock of the liquid medicine, and a progressive reminding mechanism with an extremely strong warning effect is achieved.

The invention discloses a culture medium for adipose-derived stem cells, and particularly relates to the technical field of stem cell culture. The culture medium comprises a basic culture medium and nerve-vascular niche components, wherein the basic culture medium comprises DMEM / F12, 10% of human-derived platelet lysate and 1% of antibiotic solution; the nerve-vascular niche comprises the following components: a Slit3 recombinant protein, a nerve growth factor-beta, a glial cell-derived neurotrophic factor, a lysyl oxidase inhibitor, dexamethasone, 3-isobutyl-1-methylxanthine and insulin. The traditional fetal calf serum is replaced by the human-derived platelet lysate, so that the risks of immunological rejection and pathogen pollution are avoided; through the synergistic effect of nerve-vessel niche factors, blood vessel maturation and innervation are promoted, programmed mechanical stretching culture is combined, the adipogenic differentiation efficiency of adipose-derived stem cells is remarkably improved, and engineered adipose tissue which is huge in lipid droplet, compact in structure and provided with a nerve-vessel network is formed.

1. The name of the design product: insulin needle dismounting device (sharps box). 2. The use of the design product: used for dismounting insulin needle on sharps box. 3. The design points of the design product: in shape. 4. The picture or photo that best shows the design points: perspective view.

Adaptive on board estimation of exogenous pharmacon responsive to transient (i.e., impermanent) physiological effects is provided. Dynamically estimating an equivalent amount of an exogenous pharmacon on board (XOB), such as insulin and / or carbohydrates, left in the subject, is based on predictions of glucose time-series data. These estimated values, such as insulin on board (IOB), are useful for diabetes management software, including decision support and / or artificial pancreas (AP) algorithms, for example.

The utility model provides a refrigeration device of an electronic refrigeration cup, which relates to the field of insulin electronic refrigeration cups and comprises a refrigeration cup component, the refrigeration cup component comprises a shell, a top cover, an inner container and a hollow layer, the refrigeration cup component is used for providing storage space for insulin, the top cover is located at the top of the shell and movably connected with the shell, and the inner container is arranged in the shell. The inner container is located in an inner cavity of the shell and fixedly connected with the shell, the hollow layer is located between the shell and the inner container, and a refrigeration assembly is installed in an inner cavity of the top cover; the semiconductor refrigeration sheet is adopted as a refrigeration source, the refrigeration device has the advantages of being high in refrigeration efficiency and response speed, effective heat dissipation of the refrigeration assembly is guaranteed through cooperation of the cooling fin and the cooling fan, the overheating phenomenon is avoided, the service life of the device is prolonged, and the temperature control chip in the integrated control panel is combined with the temperature sensor, so that the refrigeration efficiency is improved. And the temperature in the refrigeration cup can be monitored in real time and accurately controlled, so that the effectiveness and the safety of medicines are ensured.

The invention discloses a miniature insulin pump and system suitable for children and active groups, and belongs to the technical field of insulin pumps, the miniature insulin pump comprises an insulin pump body, and the two ends of the insulin pump body are connected with watchbands used for being worn by the children and the active groups; the insulin pump body comprises a shell, the interior of the shell comprises a cavity layer, a power storage layer and a control layer, the cavity layer is used for containing an injection hose and an injection needle which are sealed after disinfection, and the power storage layer comprises an insulin storage bin, a power bin and an electric power bin which are sequentially arranged; a control circuit board is arranged in the control layer; isolation absorption structures for absorbing leaked insulin are arranged between the layers and between the bins; the insulin storage tank and the micro infusion pump are connected through a magnetic suction circulation connector structure, and the micro infusion pump and the injection hose are connected through a magnetic suction circulation connector structure. The problems that an existing insulin pump is not designed for children and active groups, so that the carrying adaptability is poor, and the liquid leakage risk is high can be solved.

The invention relates to an isolated culture method of functional Chinese softshell turtle ovarian granular cells. The isolated culture method comprises the following steps: S1, collecting ovarian tissues: collecting Chinese softshell turtle ovarian tissues; s2, separating and collecting a granular cell layer; s3, digesting and collecting granular cells; s4, primary culture of granulosa cells: transferring the collected cells into a cell culture bottle containing 4 ml of a complete culture medium for primary culture, wherein the complete culture medium comprises the following main components: a DMEM culture medium, 15% of fetal calf serum, 1% of penicillin streptomycin and 1% of an insulin-transferrin-selenium additive; and S5, subculturing the granular cells. The isolated culture method and the culture medium system provided by the invention are simpler and more time-saving and labor-saving; according to the method, the aromatase CYP19A1 can be expressed normally, the characteristics of the granular cells are kept, the aromatase CYP19A1 can be expressed normally, it is indicated that the in-vitro cultured cells can keep the functionality of the cells, and the in-vitro proliferation capacity of the granular cells is improved to a great extent.

The present disclosure relates to methods of treating, preventing, or modulating diabetes in a patient in need thereof using a device for transplanting cells into a host body, specifically a method of treating diabetes in a patient in need thereof, comprising: implanting a device in the patient, wherein the device comprises: a porous scaffold comprising an immunologically compatible polymer mesh forming the walls of at least one chamber, an opening at either or both of a proximal end and a distal end of the chamber, at least one removable, non-porous plug configured to be positioned within the lumen of the at least one chamber, maintaining the device in the patient's body until the device is infiltrated with vascular and connective tissues; and infusing the chamber with cells, wherein at least some of the cells express insulin

Disclosed are systems and methods for determining a dose of insulin that compensates for past carbohydrates consumed, adjusts for blood glucose (BG) trend as well as BG value, and mitigates potential inaccuracies associated with double-counting or overcorrecting, where safeguards are applied to the determination of the insulin dose.

The present invention provides methods and compositions for the treatment of chronic lung disease (CLD), comprising administering to a subject in need of treatment a composition comprising insulin-like growth factor-1 (IGF-1).

The utility model belongs to insulin pen needle field, specifically comfortable big plane insulin pen needle, including outer needle cap, the inner chamber fixed connection of outer needle cap has pen needle body, the top of outer needle cap is provided with expansion mechanism, the utility model discloses a rotation ring piece, makes rotation ring piece to be able to smoothly pass through the extension block that is pulled to the rotary plate and the rotating rod, the extension block can smoothly with the center of the mounting rod circle as the center, to the center of the fixed ring piece's one side that is far away from rotating, further in the rotation to certain distance after, through the contact of self and people's skin, increase the stability of pen needle body when using, reached when pen needle body is inserted to people's body inside, pen needle body is not easy when injecting insulin, take place big amplitude's shake or shake, thereby reduced because pen needle body is not enough stable when using self, and increased people's painful feeling condition, increased the comfortable feeling of pen needle body when using.

A C-terminal fragment of insulin-like growth factor binding protein 2 (IGFBP2) containing 3 cysteine residues has been shown to spontaneously self-assemble into nanotube (NT) structures. These NTs can encapsulate drugs during the assembly process and then deliver their cargo intracellularly following binding to interns and endocytosis. The integrin-dependence of this process is mediated by the presence of a naturally occurring RGD motif within the peptide sequence. A particular advantage of such NTs is that by encapsulating small molecule drugs, tissues are protected from their adverse effects. Here, the use of NTs to deliver small drugs and biologics across the blood-brain barrier (BBB) by penetrating the CNS and delivering their cargo is described.

Disclosed is an acylated insulin, a pharmaceutical preparation thereof, a pharmaceutical composition thereof with a long-acting GLP-1 compound, and a medical use of the acylated insulin, the pharmaceutical preparation and the pharmaceutical composition. Compared with insulin degludec or other insulin derivatives, the acylated insulin has an unexpected, significantly increased drug effect, a long duration of action, a long in vivo half-life, an excellent bioavailability, as well as excellent physical and chemical stabilities.

The present disclosure relates to a method of treatment or prophylaxis of a preterm infant by administering intravenously a therapeutic amount of a composition comprising IGF-1 and IBGBP-3 in combination with a therapy selected from the group comprising: caffeine, fentanyl, fluconazole, gentamicin, insulin, midazolam, morphine, low dose norepinephrine, vancomycin concentrations of 5 mg / ml or less, parenteral nutrition (or a composition for the same use) and combinations thereof.

Techniques disclosed herein relates generally to default carbohydrate consumption counts based on population carbohydrate consumption models. In one example, the techniques involve receiving, from a device, at least one characteristic of a person; accessing a population carbohydrate consumption model which relates the at least one characteristic with carbohydrate intake for a population; determining, based on applying the population carbohydrate consumption model to the at least one characteristic, at least one of: a preliminary carbohydrate count for the person or a default carbohydrate consumption count for the person; and causing delivery of insulin to the person based on communicating, to the device, at least one of: the preliminary carbohydrate count for the person or the default carbohydrate consumption count for the person.

The invention belongs to the technical field of medical biological dressings, and provides a composite dressing with oxygen release and insulin delivery functions and a preparation method thereof.The composite dressing comprises a supporting base layer, a function slow release layer and a protective film layer which are sequentially compounded from bottom to top; the supporting base layer is a medical porous polyurethane film modified by plasma; the functional sustained release layer is composed of hyaluronic acid-chitosan composite gel, oxygen release microspheres and insulin liposome; the protective film layer is a polyethylene terephthalate film of which the surface is provided with a sawtooth edge easy to tear; through an integrated three-layer patch structure, by combining the synergistic effect of PLGA microsphere controlled-release oxygen, liposome low-molecular chitosan insulin delivery and gel matrix, the skin injury microenvironment is effectively improved, inflammation is inhibited, and the healing time is shortened.

The invention provides a special culture medium for prostate cancer organoid and a culture method thereof, and belongs to the technical field of biological medicines. The special culture medium for the prostatic cancer organoid is composed of a basic culture medium and a culture additive, and the basic culture medium does not contain exogenously added Noggin recombinant protein and R-spondin recombinant protein. The culture additive is prepared from a basic fibroblast growth factor, a keratinocyte growth factor, a fibroblast growth factor 10, insulin, a p38MAPK signal channel inhibitor, an insulin-like growth factor 1, B27, nicotinamide, N-acetylcysteine, hydrocortisone, a ROCK kinase inhibitor, retinoic acid, interleukin 6, prostaglandin E2, a eriocin and androgen. According to the special culture medium for the prostatic cancer organoid, the culture success rate is increased while the cost is reduced.

Provided is a pharmaceutical combination for preventing or treating a neurodegenerative disease. The pharmaceutical combination includes a first agent being an insulin sensitizer and a second agent being a lipid metabolism modulator. Also provided is a method for preventing or treating a neurodegenerative disease in a subject in need thereof by administering the first agent and the second agent to the subject, thereby reducing the visceral adiposity and the accumulation of amyloid β peptides in the subject.

The application discloses a pH response core-shell structure encapsulated insulin nanomicelle and a preparation method thereof. The encapsulated insulin mass accounts for 91% of the total mass of the insulin dissolved in an acidic aqueous solution, and the encapsulated insulin mass accounts for 13% of the mass of the encapsulated insulin nanomicelle. The average particle size of the micelle is 190 nm, and the Zeta potential is -19 mV. The nanomicelle is stable, the encapsulation efficiency and the drug loading capacity of the encapsulated insulin are 91% and 13% respectively, the cumulative release of the insulin mass percentage in a simulated human gastric juice environment with pH=1.2 for 2 h, 4 h and 10 h is 12%, 14% and 20% respectively, and the release of the insulin mass percentage in a simulated human intestinal juice environment with pH=7.4 for 2 h and 10 h is 55% and 77% respectively. Compared with the pH response block located in the core of the encapsulated insulin nanomicelle, the pH response block located in the shell of the encapsulated insulin nanomicelle has high stability in the simulated human gastric juice and has a slow-release behavior in the simulated human intestinal juice, and is more suitable for the bioavailability of the oral insulin of the human body.

The present invention relates to a composition comprising: an insulin derivative comprising at least one arylboronic acid or arylboroxol; and methionine, and to the use of the composition in the treatment of diabetes mellitus, including type 1 diabetes mellitus and type 2 diabetes mellitus.