337 results about "Ex vivo" patented technology

Modular mucosal tissue bioreactors combined with bioartificial mucus provide a high-fidelity, medium-throughput platform for evaluating CF treatments and modeling host-pathogen biology, a stepping stone towards better options for HEMT-nonresponsive patients. Whole lung ex vivo Cystic Fibrosis model system allows for investigation of candidate CF therapies.

The present invention relates to compounds of formula (I) useful for the ex vivo, in vitro or in vivo degradation of BRAF by the ubiquitin proteolysis pathway. The invention also provides pharmaceutically acceptable compositions comprising the compounds, and methods of using the compositions in the treatment of various diseases, disorders, and / or conditions.

The invention discloses bacillus tequilensis and application thereof, and belongs to the technical field of biology. The preservation number of the bacillus tequilensis is CCTCC (China Center For Type Culture Collection) NO: M 20251909, and the bacillus tequilensis has a remarkable inhibition effect on rice sheath blight disease pathogenic bacteria (Rhizoctonia solani). An in-vitro confrontation culture and potting verification method is adopted to prove that the strain can inhibit the growth of germs, and a plate confrontation experiment shows that the hypha growth inhibition rate of the strain to YM-Q-7 reaches 70.36%. The bacterial suspension (1 * 10 < 8 > CFU / ml) of the strain is used for performing root irrigation treatment on potted rice, the prevention and treatment effect reaches 28.85%, and a good application prospect is provided for prevention and treatment of rice sheath blight disease.

Embodiments of the present invention include methods of targeting acidosis (low pH) within the tumor microenvironment (TME) through the use of cathodic electrochemical reactions (CER). Low pH is oncogenic by supporting immunosuppression. Electrochemical reactions create local pH effects when a current passes through an electrolytic substrate such as biological tissue. Electrolysis has been used with electroporation (destabilization of the lipid bilayer via an applied electric potential) to increase cell death areas. However, the regulated increase of pH through only the cathode electrode has been ignored as a possible method to alleviate TME acidosis, which could provide substantial immunotherapeutic benefits. Here, ex vivo modeling shows that CERs can intentionally elevate pH to an anti-tumor level and that increased alkalinity promotes activation of naïve macrophages. Embodiments of the invention include pairing CER treatment protocols with existing electric field-based cancer therapies or use as a stand-alone therapy.

The present invention provides preparations of MSCs with important therapeutic potential. The MSC cells are non-primary cells with an antigen profile comprising less than about 1.25% CD45+ cells (or less than about 0.75% CD45+), at least about 95% CD105+ cells, and at least about 95% CD166+ cells. Optionally, MSCs of the present preparations are isogenic and can be expanded ex vivo and cryo-preserved and thawed, yet maintain a stable and uniform phenotype. Methods are taught here of expanding these MSCs to produce a clinical scale therapeutic preparations and medical uses thereof.

The invention provides arthrobacter HJ1 for preventing and treating fungal root rot of polygonatum sibiricum and application of the arthrobacter HJ1, and belongs to the technical field of disease prevention and treatment. The preservation number of the arthrobacterium HJ1 is CCTCC (China Center For Type Culture Collection) NO: M20251843 The arthrobacter HJ1 has a remarkable inhibition effect on the pathogenic bacterium fusarium oxysporum of the rhizoma polygonati root rot. The arthrobacter HJ1 has a remarkable inhibition effect on the rhizoma polygonati root rot under an in-vitro condition, and can remarkably reduce scab expansion of the rhizoma polygonati root rot caused by fusarium oxysporum. In a field test, by using the arthrobacter HJ1, the morbidity of the rhizoma polygonati root rot can be remarkably reduced, and the control effect on the rhizoma polygonati root rot is improved. A safety test shows that the arthrobacter HJ1 has no adverse effect on the growth of the polygonatum sibiricum and can be safely used for preventing and controlling root rot fungal diseases of the polygonatum sibiricum. The arthrobacter HJ1, as a beneficial biocontrol bacterium for polygonatum sibiricum root rot fungal diseases, is of great significance to healthy and green development of the polygonatum sibiricum industry.

The invention belongs to the technical field of biomedical imaging, and particularly discloses an in-vivo imaging method of an SAPAP3 gene defect disease model, which is characterized in that an effective dose of an S1PR1 receptor targeted radioactive probe is injected into the body of the SAPAP3 gene defect disease model. According to the method, an S1PR1 receptor targeting radioactive probe is utilized, the technical bottleneck that in-vivo and dynamic observation of the S1PR1 receptor cannot be achieved through an existing in-vitro technology is solved, and an indispensable visual tool and a quantitative evaluation means are provided for studying the neurobiological mechanism of related mental diseases such as obsessive-compulsive disorder and accelerating research and development of related drugs.

The present application relates to the field of plant tissue culture technology, and particularly discloses a lily stem cell abundance ex vivo regulation method. The lily bulb is used as the starting material, stem cell magnitude amplification and regulation culture can produce lily tissue organ materials with high stem cell abundance. The present application provides sterile lily materials with high stem cell abundance, which has the characteristics of strong regenerative ability, high proliferation rate, etc., is the basis for protoplast preparation and high-efficiency regeneration, and is suitable for different lily varieties, and provides technical support for cell engineering breeding, seedling scale breeding, etc.

This disclosure generally relates to super minimal transposon inverted repeat sequence (ITR) polynucleotides, compositions comprising the polynucleotides and methods of using compositions comprising the polynucleotides for the ex vivo and in vivo delivery of nucleic acids to cells, in particular, in vivo delivery of therapeutic genes to treat genetic disorders or diseases.

An ex vivo test system 10 is constituted of a fluid circuit that circulates a prescribed circulation liquid by simulating the blood circulation state of a human body, and comprises a circulation liquid maintenance unit 17 that maintains, in the same manner as the blood environment in vivo, chemical factors in the circulation liquid, which affect the performance evaluation of a test object S. The circulation liquid maintenance unit 17 comprises: a replenishing liquid unit 23 that maintains the chloride ion concentration and protein concentration of the circulation liquid at desired values by injecting a replenishing liquid composed of an electrolyte-free isotonic solution into the fluid circuit; and a carbon dioxide supply unit 24 that maintains the pH of the circulation liquid at a desired value by supplying carbon dioxide into the fluid circuit, the state of the circulation liquid being maintained while compensating for the effect of moisture loss during circulation of the circulation liquid.

The present disclosure provides methods of amplifying tissue samples derived from humans for purposes of treating certain diseases. The method comprises: isolating a small amount of associated tissue by biopsy or other tissue acquisition methods; the tissue is amplified in vitro, and meanwhile, natural functions are reserved; and re-implanting the amplified tissue to alleviate human disease. One example includes ex vivo expansion of human pancreatic islet tissue and reimplantation of the expanded cells into an individual with Type 1 diabetes for treatment and long term relief or cure of disease.

The invention discloses an establishment method of a cherry rootstock Gisela 6 leaf in-vitro regeneration system and transgenic application thereof, and belongs to the field of plant biotechnology and genetic engineering. According to the regeneration system established by optimizing the formula of the culture medium, the regeneration frequency of the 'Gisela 6' leaf is stabilized at a relatively high level, and the quality of the regenerated material is remarkably improved. The callus generated by induction of the method provided by the invention is compact and strong in browning resistance, and the transgenic callus capable of stably expressing RUBY is obtained by a leaf disc transformation method; meanwhile, vitrification of regenerated buds is effectively inhibited, robust plants easy to transplant are obtained, and technical support is provided for precise improvement and breeding speed.

The present disclosure relates to an in vitro or ex vivo use of ALDH3A2 downstream noncoding variant, wherein said ALDH3A2 downstream noncoding variant is a sequence identical to a sequence selected from the list consisting of SEQ. ID 2, SEQ. ID 3, SEQ. ID 4, as a biomarker for determining the color phenotype of a Psittaciforme species.

The invention discloses an application of trichoderma harzianum in biological prevention and treatment of wild apple tree canker, the classification name of the trichoderma harzianum is Bacillus velezensis Rifai, the preservation unit is China General Microbiological Culture Collection Center (CGMCC), the preservation number is CGMCC NO. 42231, and the preservation time is September 29, 2025. Experimental results show that the trichoderma harzianum has remarkable antagonistic activity on parasitic cyst, apple cyst, nutshell cyst, cyst hormaechei, prunus cyst and pear cyst, is environment-friendly and pollution-free, and also provides a safe and reliable new way for preventing and treating the valsa mali of the wild apples; in addition, the research also finds that the fermentation product of the trichoderma harzianum has a certain biocontrol effect on the in-vitro branches infected by the physosporium piricola, so that the trichoderma harzianum and the fermentation product of the trichoderma harzianum provided by the invention can be used for preventing and treating the wild apple tree canker, and have good development and application prospects.

The invention relates to a method for inducing doubling cultivation of polyploidy by girdling calluses of camellia oleifera branches, the influence on tree vigor is reduced through girdling, the efficiency of regeneration buds is improved through thorough removal of cambium, the bottleneck in the prior art is overcome, chimera-free polyploidy material induction can be realized without in-vitro tissue culture, and the method is suitable for large-scale popularization and application. The method is simple and convenient to operate and low in cost, can effectively avoid the problems of rooting difficulty of tissue culture materials, transplanting loss of tissue culture seedlings and the like, provides a new technical approach for woody plant polyploid breeding, and has important popularization and application values.

This invention relates to a method and application for enriching circulating tumor cells (CTCs) based on ambient temperature mechanical perfusion technology, belonging to the field of biomedical technology. The method comprises the following steps: flushing the liver resected during liver transplantation via the portal vein and hepatic artery, connecting it to an ambient temperature mechanical perfusion loop, and performing in vitro perfusion using simulated blood perfusion fluid; when perfusion exceeds 2 hours and the lactate content drops to <5 mmol / L, collecting the simulated blood perfusion fluid; and sequentially subjecting the simulated blood perfusion fluid to centrifugation, red blood cell removal, and white blood cell removal to obtain enriched circulating tumor cells (CTCs) for liver cancer. A human liver cancer ex vivo organ perfusion model is constructed using NMP technology to fully recreate the tumor microenvironment and CTC generation process, and the large volume of perfusion fluid is processed to achieve efficient enrichment of CTCs.

The disclosure relates to formulations and methods for the in vitro testing of ocular irritants. It was discovered that adding an antioxidant formulation to in vitro ocular irritation tests, including for example, a biochemical ocular irritation test, a reconstituted human corneal epithelium (RhCE) ocular irritation test and an excised eye depth of injury (Dol) test, substantially reduces the rate of false positives without diminishing test sensitivity, resulting in more accurately predicting ocular irritancy of test substances. More particularly, the disclosed method employs relatively high physiologic concentrations of one or more antioxidants that are normally present in tears. In a variation, much higher concentrations of one or more antioxidants may provide protection against in vivo exposure to ocular irritants.

The present application belongs to the field of agricultural biotechnology, and relates to a Hainan Catimor coffee tissue culture breeding method, which takes the middle and upper section of the hypocotyl of a Hainan Catimor coffee sterile seedling as an explant, carries out, after sterile treatment, five stages of callus induction, adventitious bud differentiation, cluster bud proliferation, rooting culture and seedling raising and transplanting in turn; each stage adopts a modified MS culture medium, and is matched with a special exogenous hormone combination, and meanwhile, the culture temperature is controlled to be 20-25 DEG C, the suitable light intensity and light time are controlled, and a specific concentration of PPM is added to inhibit pollution. The present application has the advantages of fast induction speed, stable culture effect, compact and non-deformed callus, healthy and neat adventitious buds and cluster buds, effective reduction of the proportion of abnormal seedlings and weak seedlings, significant improvement of the in-vitro regeneration efficiency and tissue culture seedling quality of Hainan Catimor coffee, simple operation, strong repeatability, suitability for large-scale factory breeding of Hainan Catimor coffee, and important application value.

The invention discloses an in-vitro biological neural network accurate regulation and control method and system based on a microelectrode array, and the method comprises the steps: S1, culturing and planting neuronal cells on a microelectrode array chip, and forming an in-vitro neural network with spontaneous activity; s2, preprocessing the in-vitro neural network to enable the in-vitro neural network to enter a stable function state capable of being effectively activated; s3, recording an electrophysiological signal, sampling the structure and function connection of the neural network, and dynamically selecting an optimal stimulation site; s4, applying electrical stimulation to the selected stimulation sites according to a preset regulation and control target, collecting neural network response signals in real time, and constructing a closed-loop regulation and control loop; and S5, performing quantitative analysis on the neural network based on the multi-dimensional evaluation index to form a comprehensive evaluation result, and feeding back the comprehensive evaluation result to the step S4 to optimize a subsequent stimulation strategy. According to the method, organic unification of sensing, intelligent decision making, closed-loop intervention and multi-dimensional quantitative evaluation of a neural network structure and a function state can be realized.

The invention discloses a computer device for noninvasive recognition of Parkinson's disease based on intestinal flora and a computer readable storage medium. The invention provides a computer device, which comprises a memory, a processor and a computer program stored on the memory, and the processor executes the computer program to realize the following steps: S1, receiving data: receiving sample data, the sample data being relative abundance of each genus in 11 genus in an in-vitro fecal sample of a subject; s2, inputting data: inputting the sample data into a Parkinson's disease prediction model; s3, outputting a result: outputting a probability value of the illness condition of the Parkinson's disease of the subject through the Parkinson's disease prediction model; and then the Parkinson's disease condition of the subject is obtained based on the probability value. The method shows obvious superiority in the aspects of non-invasive prediction of PD, personalized medical support, cost effectiveness and the like, and has important clinical application value and research prospect.

The present invention provides a novel use and methods comprising antibodies, or antigen-binding fragments thereof, which bind to a CCR7 receptor for use as a novel therapeutic agent in prevention and / or treatment of graft versus host disease (GVHD), preferably in hematopoietic stem cell transplantation (HSCT), more preferably allogeneic hematopoietic stem cell transplantation. GVHD of the invention can be acute (aGVHD) and / or chronic (cGVHD), preferably acute. The antibodies and antigen-binding fragments are capable of selectively depleting ex vivo or in vitro immune cells expressing CCR7 and are capable in vivo of selectively killing immune cells expressing a CCR7 receptor and of impairing / blocking migration and of activation of said immune cells, which are involved in the development and evolution of GVHD. The use of said antibodies for depleting, killing and impairing / blocking migration and activation of immune cells expressing CCR7 cells is disclosed, thus providing an alternative therapy for preventing and treating GVHD in both acute and chronic types.

The invention discloses a thrip resistance identification device and application. The device comprises an inoculation barrel, a preservation substrate is laid at the bottom in the inoculation barrel, an isolation structure with one or more through holes covers the preservation substrate, and a sealing structure with one or more air holes covers an opening in the top of the inoculation barrel. When the device is used, the whole plant does not need to be cultivated, in-vitro flowering branches can be picked for identification, rapid screening of large-scale germplasm resources can be achieved, and repeatability and consistency of experimental results are good. Meanwhile, when the device is used, buds and functional leaves of in-vitro flowering branches are reserved, the actual feeding part of the Frankliniella occidentalis is simulated, and compared with in-vitro leaf inoculation, the real resistance level can be better reflected. The device has the advantages of easily available materials, low cost and strong operability, can be used for identifying the resistance of the Frankliniella occidentalis of various materials such as chrysanthemum and related wild species, cultivated species, breeding offspring and the like, provides a high-quality parent material for insect-resistant breeding, can be used for researching an insect-resistant mechanism, and is suitable for popularization and application in basic breeding units.

The invention discloses a cell membrane in-situ drug membrane protein target screening method based on variable configuration DNA, and belongs to the technical field of biological medicine. Two pairs of functionalized nucleic acid structures are constructed, the first pair is S1S2 semi-complementary DNA double strands, the second pair is S3S4 DNA-RNA hybrid double strands, and the S1S2 structure is covalently coupled to a non-natural amino acid site-directed modified membrane protein through a click chemical reaction in a living cell to realize tagging of the membrane protein; when the candidate drug coupled in S3 interacts with the membrane protein, triggering an allosteric response system to release the tagged chain in S1; the released DNA tag is subjected to PCR amplification and sequencing, and accurate identification and analysis of a membrane protein target are realized according to a tag sequence. The invention provides a high-specificity and non-in-vitro drug membrane protein target screening technology. According to the technology, active compound discovery or accurate screening of drug membrane protein targets in a living cell in-situ environment can be realized.

The invention discloses a method for realizing efficient in-vitro regeneration of tea tree cotyledons through a somatic embryo indirect generation way, and belongs to the technical field of plant tissue culture. The tea tree somatic embryo indirect generation system is successfully constructed by screening culture conditions, a culture medium formula and other methods, and a foundation is laid for construction of a tea tree genetic transformation system and industrialized seedling production; according to the method, immature cotyledons of tea trees are used as explants, the cotyledons are induced to generate calluses, then the calluses are proliferated and differentiated to generate somatic embryos, and the somatic embryos are further differentiated to form complete plants, so that efficient and synchronous propagation of tea tree seedlings is realized, and technical support is provided for high-quality variety propagation and new variety breeding of tea trees; the problems of low propagation coefficient, long growth cycle, serious descendant degeneration and the like in traditional seed propagation of tea trees are solved, and the technical bottlenecks of serious explant browning, high bacterial contamination rate, poor plant growth synchronism and the like in other tissue culture technologies are solved.

The invention discloses a method for constructing astrocytes serving as a smoke disease model, and belongs to the technical field of crossing of stem cells and neuroscience. The method comprises the following steps: S1) reprogramming CD34 + cells in in-vitro PBMCs (peripheral blood mononuclear cells) of smoke disease patients carrying and not carrying RNF213p.R4810K mutation to obtain induced pluripotent stem cells; and S2) directionally inducing and differentiating the induced pluripotent stem cells into astrocytes through a neural progenitor cell way, wherein the obtained astrocytes are the astrocytes capable of being used as the smoke disease model. The astrocyte model prepared by the invention can be used for researching pathogenesis, nerve-blood vessel interaction process and blood-brain barrier (BBB) related functions of smoke diseases, and can be further applied to molecular typing of diseases and in-vitro function evaluation of candidate drugs.

The invention provides a corydalis pygmaea terminal bud organogenesis and in-vitro rapid propagation method, and belongs to the technical field of plant tissue culture. Comprising the following steps: by taking a terminal bud of a corydalis pygmaea aseptic seedling as an explant, sequentially performing adventitious bud differentiation culture, cluster bud multiplication culture and rooting induction to obtain a corydalis pygmaea tissue culture seedling, pretreating a root system of the corydalis pygmaea tissue culture seedling with nano-zinc oxide, and transplanting the corydalis pygmaea tissue culture seedling. According to the method, the terminal bud is used as the explant, the organ occurrence rate and the propagation coefficient are remarkably increased by optimizing the culture medium formula and the culture conditions, the induction rate reaches 85% or above, the browning rate is controlled to be 10% or below, the proliferation multiple per four weeks can reach 11-14 times, the rooting rate exceeds 90%, rapid and efficient propagation of corydalis pygmaea is achieved, reliable technical support is provided for large-scale production, and the method has a wide application prospect. The problem of raw material supply can be effectively solved, and a foundation is laid for industrial production of active ingredients.

The application belongs to the field of in vitro rapid propagation of Chinese medicine seedling, and particularly relates to a high-efficiency liquid suspension culture method of pinellia ternate, which comprises obtaining of pinellia ternate explants under sterile conditions, induction of embryogenic callus, liquid suspension culture, somatic embryo development and maturation, somatic embryo germination and transplanting of regenerated seedlings. By using the method, the induction rate of pinellia ternate embryogenic callus can reach 81.6%, stable mature cotyledon embryos can be obtained, and the embryos can be regenerated into complete plants, a high-efficiency and stable regeneration system is successfully established, favorable conditions are created for industrial production of pinellia ternate fine seedlings, and a good foundation is laid for genetic transformation of pinellia ternate.

The application provides a method for improving the germination rate and storage capacity of kiwi pollen, and belongs to the technical field of forest tree breeding. The method comprises the following steps: S1, placing the pollen of male kiwi flowers in 24-28 DEG C light treatment for 10-14 hours; S2, spreading the pollen treated in S1 on an in vitro germination medium for light culture; S3, observing the germination state of the pollen and calculating the survival rate of the pollen. According to the method, the pollen is first placed in 24-28 DEG C light pretreatment for 10-14 hours, so that the germination rate of the pollen is significantly higher than that of fresh pollen, and compared with the pollen without pretreatment, the method can further improve the pollen storage capacity of kiwi pollen under 4 DEG C and-20 DEG C storage conditions, and the decrease range of the pollen germination rate during storage is significantly reduced.

The field of the invention is in vitro assays for the production of in vitro organ culture systems and the use of such in vitro organ culture systems for screening assays in drug screening, patient selection and personalized medical context. More specifically, the present invention relates to an in vitro method of establishing a tumor organoid from a cancer tissue sample obtained from a subject, an in vitro method of providing immune cells activated by an autologous tumor organoid, a method of preparing an in vitro organ culture system for mimicking interaction of a tumor with an immune system of a subject, and a method of preparing an in vitro organ culture system for mimicking interaction of a tumor with an immune system of a subject. The invention relates to an in vitro organ culture system, and to an in vitro organ culture system produced thereby, to a method for determining the reactivity of a tumor to at least one immunooncology drug therapy using said in vitro organ culture system, and to a kit for preparing an in vitro organ culture system that mimics the interaction of a tumor with the immune system of a subject, as disclosed herein.

The application provides a kind of based on digestive tract tissue protein expression abundance determination method and system, applied to digestive tract tissue image processing technical field. Including: based on the PVDF membrane image after color development of digestive tract tissue acquisition in vitro, contain at least one protein standard band and the protein band of multiple target proteins;When determining the presence of electrophoresis distortion according to the band of target protein standard, construct pixel space coordinate system;The target image is obtained by angle correction and linear repair to PVDF membrane image, and the protein band corresponding to multiple target proteins in target image is in horizontal alignment state under pixel space coordinate system;Determine the development area of each target protein in target image and the irregular region of interest wrapped the protein band corresponding to multiple target proteins, and for each target protein, the expression abundance of target protein is obtained by analyzing the development area and the irregular region of interest, and the accuracy of the protein expression abundance is higher.