1194 results about "Ex vivo" patented technology

Provided are ex vivo systems and methods of predicting the response of a drug or other agent on a tissue. In some embodiments, the systems and methods comprise cutting a tissue into tissue fragments, adding a drug or other agent to the tissue fragments based on an estimated tumor content, and performing an ex vivo measurement on the tissue fragments.

Ionizable cationic lipids, methods for synthesizing the same, intermediates useful in synthesis of the ionizable cationic lipids and methods of synthesizing the intermediates are disclosed. The ionizable cationic lipids are useful as a component of lipid nanoparticles (LNP), which in turn can be used for the delivery of nucleic acids into cells in vivo or ex vivo. LNP compositions are also disclosed, including LNP comprising a functionalized lipid to enable conjugation of a binding moiety, and targeted LNP (tLNP), that is an LNP in which a binding moiety has been conjugated to the functionalized lipid and can serve as a targeting moiety to direct the tLNP to a desired tissue or cell type.

The invention belongs to the technical field of biology, and particularly relates to an extraction method of polydeoxyribonucleotide, which comprises the following steps: carrying out homogenate treatment on pretreated in-vitro testis tissue, filtering the obtained homogenate, adding a pancreas extract into the filtrate, and stirring to obtain enzymatic hydrolysate; and precipitating protein in the enzymatic hydrolysate, removing the protein, adding sodium acetate for treatment, adding absolute ethyl alcohol for precipitation, centrifuging, washing the obtained precipitate, and drying to obtain the product. Common trypsin and lipase in fish pancreas are beneficial to release of DNA, the fish pancreas extract is applied to extraction of the polydeoxyribonucleotide, the extraction time can be remarkably shortened, the yield can be increased, meanwhile, nuclease in the fish pancreas extract can effectively degrade DNA, and small-fragment polydeoxyribonucleotide is obtained.

The invention belongs to the field of biological prevention and control of plant diseases, and particularly discloses a biocontrol bacterium bacillus velezensis and application thereof in preparation of a biocontrol inoculant for preventing and controlling pepper phytophthora blight, the strain is bacillus velezensis Y426-7, is preserved in China General Microbiological Culture Collection Center (CGMCC) on September 23, 2024, and has the preservation number of CGMCC NO.32041, and the preservation number is CGMCC NO.32041. The biocontrol bacterium is bacillus velezensis Y426-7. The bacillus velezensis is preserved in No. 3, Yard 1, Beichen West Road, Chaoyang District, Beijing, and can be used for preparing a biocontrol inoculant for preventing and treating pepper phytophthora blight. Compared with the prior art, the bacillus velezensis Y426-7 obtained by the invention has an obvious antibacterial effect on phytophthora capsici and various crop phytophthora blight bacteria under an indoor in-vitro condition; furthermore, the 2.5 * 10 < 9 > CFU / g wettable powder prepared from the bacillus velezensis Y426-7 has a relatively good prevention and treatment effect and a certain yield increasing effect on the pepper phytophthora blight in the field.

The invention discloses a serum-free culture system and culture method of NK cells. The invention firstly discloses a serum-free culture system of the NK cells, wherein the serum-free culture system comprises a coating solution, an amplification culture medium, an activation culture medium and autologous plasma; the invention further discloses a culture method for NK cells by using the serum-free culture system. The method for culturing the NK cells by using the serum-free culture system disclosed by the invention reduces the culture cost of the NK cells, can simply, quickly, efficiently and safely obtain a large number of NK cells with high activity, high purity and strong killing ability in vitro, provides a better cell source for clinical application of the NK cells, and promotes further development of an NK cell treatment technology.

The invention discloses a biological in-vitro sample aging quality control detection method, and relates to the technical field of biological samples, and the method specifically comprises the following steps: screening metabolic markers based on biological physiological characteristics, and constructing an in-vitro quality attenuation model; collecting and preprocessing sample data; detecting the concentration of the metabolic marker through detection equipment; inputting marker concentration data into the model for dynamic comparison; and outputting aging classification, and guiding experiment scheduling operation. According to the method, effective use time efficiency is adopted for rapid judgment, the problem that experimental data is influenced by metabolic degradation of a sample after in-vitro is solved, time efficiency grading can be completed in a short time through multi-marker dynamic comparison and a machine learning model, the method is remarkably superior to a traditional method, and by combining a micro-fluidic detection device and an LSTM model, the detection accuracy is greatly improved. According to the method, the samples can be scheduled to proper experiment operation according to the effective time limit of the samples, waste of the samples is avoided, and after failure samples are detected, a user can be reminded to collect the samples again, so that the experiment process is prevented from being delayed.

The invention discloses a method for obtaining pigment cells through in-vitro culture of plectropomus leopardus skin tissues. The method comprises the following steps: step A, preparing a primary cell culture solution; step B, pretreating the plectropomus leopardus skin tissues to obtain pretreated fish skin tissues; step C-1, carrying out a digestion reaction on the pretreated fish skin tissue by an enzymolysis method to obtain an explant; and step C-2, carrying out in-vitro culture on the explant through an explant method to obtain the plectropomus leopardus skin tissue primary pigment cells. The plectropomus leopardus skin tissue is successfully separated and cultured into a plectropomus leopardus skin tissue primary pigmented cell group by combining an enzymolysis method and an explant method, so that coexistence culture of several kinds of pigmented cells is realized. Raw materials are provided for exploring the self function and gene regulation and control mechanism of the pigmented cells, and preconditions of in-vitro verification experiments are provided for further exploring the influence of the pigmented cells on the body color. Meanwhile, a foundation is laid for breeding of improved varieties of the plectropomus leopardus.

The invention discloses a marine fish myoblast in-vitro proliferation method. A basal culture medium is used as a background culture medium of a culture medium; the culture medium contains IGF2 protein with the final concentration of 20-200 ng / mL; the IGF2 protein is derived from plectropomus leopardus. The culture medium is used for culturing the passage cells to obtain the myoblasts proliferated in vitro of the plectropomus leopardus, the in-vitro culture of the myoblasts of the plectropomus leopardus is successfully realized, the number of the myoblasts reaches 30000, and the in-vitro proliferation rate reaches 87%. The invention provides raw materials for exploring the self function of myoblasts and gene regulation and control mechanism, provides preconditions for an in-vitro certification experiment for further exploring the influence of the myoblasts of marine fishes on the growth speed, and also lays a foundation for improved variety breeding of the marine fishes.

Provided herein are systems, compositions, and methods for generating immunogenic peptides or epitopes from tumor associated antigens (e.g., in vivo or ex vivo). Polynucleotides (e.g., genes) encoding the tumor associated antigens may be edited at selected target sites by nucleobase editors comprising a catalytically-inactive Cas9 and a cytosine deaminase, leading to the expression of heteroclitic or cryptic peptides that are more immunogenic than the native peptide derived from the tumor associated antigens. The heteroclitic or cryptic peptide elicit strong tumor-specific immune response (e.g., T-cell response or B-cell response), which inhibits tumor growth and metastasis.

The invention discloses an anti-digestion polysaccharide-based food colon simulated glycolysis and multi-dimensional online analysis system, and belongs to the technical field of food. According to the method, glycolysis simulation of each colon part is completely carried out according to a human body digestion and glycolysis process and a physiological environment, and intestinal mucosa pellets are added to simulate a human body intestinal mucosa environment, so that the degradation process of partial microorganisms of the human body colon for resisting digestion of polysaccharide-based food is simulated under an in-vitro condition; and the glycolysis condition of the digested polysaccharide-based food entering the colon can be observed on line, and the device fits the real physiological environment of people.

Disclosed are methods, compounds, and compositions useful for increasing autophagy and promoting longevity. The methods, compounds, and compositions relate to urolithins and urolithin precursors and use thereof. Certain urolithins are represented by Formula I, while certain urolithin precursors are represented by Formula IV. The urolithin may be urolithin A, urolithin B, urolithin C, or urolithin D. The urolithin precursor may be ellagic acid or an ellagitannin. The methods include in vivo, ex vivo, and in vitro uses of the compounds and compositions.

The embodiment of the invention provides a three-dimensional water culture device for anther culture seedlings based on a rice anther in-vitro culture system, and relates to the field of water culture equipment. Comprising a support and a plurality of hydroponic tanks, and a plurality of brackets used for placing the hydroponic tanks are mounted on the support; the hydroponic tank is of a hollow structure with an open top, a top plate capable of closing the hydroponic tank is placed at the top of the hydroponic tank, a plurality of seedling culture bases are installed on the top plate, a lifting mechanism is installed in the hydroponic tank, and the lifting mechanism can make the top plate ascend or descend. The rice seedling root system can be automatically separated from water, inspection is facilitated, the working difficulty is reduced, and the efficiency is improved; the growth space of rice seedling root systems can be limited, the root systems are prevented from expanding all around and growing obliquely, and the follow-up transplanting survival rate is increased; in addition, when rice seedlings leave water, the root system movement can be prevented from being hindered, and it can be guaranteed that the root system is not damaged when reset.

Anti-CD90 antibodies, binding fragments, and uses thereof are described. The provided antibodies and binding fragments can be used to isolate CD90 cells or to target such cells ex vivo or in vivo for a research, a diagnostic, or a therapeutic purpose. Anti-CD90 antibodies and binding fragments thereof can also be used in engineered formats to create multi-domain binding molecules, antibody conjugates, recombinant receptors, or within a recombinant protein to pseudotype viral vectors.

The disclosure provides conjugates comprising a targeting moiety a lipid nanoparticle (LNP) encapsulating a therapeutic agent (e.g., payload) for delivery to immune cells. The conjugates can be delivered to immune cells ex vivo or formulated in a pharmaceutical composition and can be directly administered to a subject in need of (i.e., via in vivo administration).

The invention belongs to the field of biological medicine, and provides a protection liquid capable of preserving in-vitro animal organs or in-vitro animal tissues at normal temperature as well as a preparation method and application of the protection liquid, and the protection liquid comprises a solution containing a hemoglobin oxygen carrier or a hemoglobin carrier, an osmotic pressure regulating substance and the like. The protective liquid provided by the invention can be used for preserving or perfusing broken limbs, isolated animal organs or isolated animal tissues at normal temperature, and can also be used as a culture liquid of artificial organs and artificial tissues.

Provided are variant Cas12f4 polypeptides, fusion proteins containing variant Cas12f4 polypeptides, nucleic acids encoding variant Cas12f4 polypeptides and fusion proteins, compositions and methods related to those variant Cas12f4 polypeptides and fusion proteins, and modified host cells comprising the variant Cas12f4 polypeptides and / or encoding nucleic acids. The variant Cas12f4 polypeptides disclosed herein exhibit improved functionality, stability, and / or other desirable properties and may be used in combination with guide Cas12f4 RNAs that can be used in a variety of applications ex vivo, in vitro, and in vivo.

The field of the invention belongs to in vitro assays for producing an in vitro organ culture system, and the use of such an in vitro organ culture system for screening assays in the context of drug screening, patient selection, and personalized medicine. More specifically, the invention relates to an in vitro method of establishing tumor organoids from a cancer tissue sample obtained from a subject, to an in vitro method of providing autologous tumor organoid-activated immune cells, to a method of preparing an in vitro organ culture system for mimicking tumor interaction with an immune system of a subject, as well as to the in vitro organ culture system produced thereby, to a method for determining the responsiveness of a tumor to a treatment with at least one immune- oncologic drug applying said in vitro organ culture system, and a kit-in-parts for preparing an in vitro organ culture system mimicking tumor interaction with an immune system of a subject, as disclosed herein.

The invention relates to the technical field of agricultural breeding, in particular to a bamboo reed saline-alkaline resistant screening and stress-resistant tissue culture seedling raising method which comprises the following steps: step 1, pretreatment; step 2, dynamic salt stress screening: placing the seed stems in a mixed salt-alkali solution with gradient increasing, culturing, measuring the bud length and root length growth rate of axillary buds of the seed stems, fitting a salt concentration-growth rate curve, and extracting a semi-inhibitory concentration IC50 and a maximum tolerance concentration MTC; step 3, salt tolerance grade classification; 4, carrying out stress-resistant tissue culture seedling culture: carrying out in-vitro culture on the axillary bud explant of the high-salt-resistant variety in a culture medium, and inducing clumpy buds and rooting in combination with a salt-resistant inducer; 5, domestication and transplanting: performing gradual salt stress domestication on the tissue culture seedlings, transplanting the tissue culture seedlings into a saline-alkaline environment, and monitoring the adaptability of the tissue culture seedlings in the saline-alkaline environment. According to the method disclosed by the invention, the efficient cultivation system of the suitable-to-grow bamboo reed seedlings in the saline-alkali soil is established by fusing dynamic stress response analysis and stress resistance phenotype directional strengthening technologies.

The invention relates to the field of biological detection, in particular to a positive sample pretreatment kit and application thereof. The method comprises the following steps: providing a simple survival environment for bacteria by utilizing incubation liquid, and then utilizing the interaction of opposite electric properties between chemical magnetic beads and the bacteria, that is, when the bacteria in a positive sample are negatively charged, the chemical magnetic beads are positive magnetic beads; or when the bacteria in the positive sample are positively charged and the chemical magnetic beads are negative electromagnetic beads, the bacteria-magnetic bead compound is obtained, the operation is simple, the cost is low, the enrichment efficiency is high, the impurity interference resistance is high, the activity state of the enriched bacteria is not changed, the method can be used for subsequent mass spectrum identification and metabolic activity analysis, the application range is wide, and the method is suitable for popularization and application. Various in-vitro samples can be universally enriched, and the method has great significance on the development of the IVD field.

Disclosed are methods for producing a population of autologous engineered cells to be reinfused into a subject comprising extracorporeally transfecting an internal payload into a population of target cells from a sample obtained from the subject by incubating the population of cells with a lipid nanoparticle (LNP) and / or targeting lipid nanoparticle (tLNP) in an incubation component of an extracorporeal system for no more than 6 hours.

The invention discloses a method for controlling left and right heart compaction degree before extracorporeal circulation operation, which comprises the following steps of: installing a peristaltic pump at a left and right heart inlet of an extracorporeal circulation device in an in-vitro environment; a liquid inlet of the peristaltic pump is connected with a liquid inlet pump, and a liquid outlet of the peristaltic pump is connected with a liquid outlet pump; and finally, a first pressure measuring structure and a second pressure measuring structure are arranged on the sides, away from the peristaltic pump, of the liquid inlet pump and the liquid outlet pump correspondingly, and the pressure of the liquid inlet pump and the pressure of the liquid outlet pump are adjusted to be-180 mmHg. The negative pressure of the liquid inlet pump is adjusted through the first pressure measuring structure, and the negative pressure of the liquid outlet pump is adjusted through the second pressure measuring structure, so that the purpose of quantifying the adjusting mode is achieved, the adjusting process for controlling the left and right heart compression degree before the extracorporeal circulation operation is visualized, the adjusting result is converted into the specific negative pressure values of the liquid inlet pump and the liquid outlet pump, and the accuracy of the operation is improved. The pressing degree reference for adjusting the left center and the right center is numeralized and standardized, the adjusting difference is avoided, experience and feeling are not needed, and the adjusting mode is fast and high in accuracy.

Disclosed are methods, compounds, and compositions useful for increasing autophagy and promoting longevity. The methods, compounds, and compositions relate to urolithins and urolithin precursors and use thereof. Certain urolithins are represented by Formula I, while certain urolithin precursors are represented by Formula IV. The urolithin may be urolithin A, urolithin B, urolithin C, or urolithin D. The urolithin precursor may be ellagic acid or an ellagitannin. The methods include in vivo, ex vivo, and in vitro uses of the compounds and compositions.

The present invention provides preparations of MSCs with important therapeutic potential. The MSC cells are non-primary cells with an antigen profile comprising less than about 1.25% CD45+ cells (or less than about 0.75% CD45+), at least about 95% CD105+ cells, and at least about 95% CD166+ cells. Optionally, MSCs of the present preparations are isogenic and can be expanded ex vivo and cryopreserved and thawed, yet maintain a stable and uniform phenotype. Methods are taught here of expanding these MSCs to produce a clinical scale therapeutic preparations and medical uses thereof.

According to one embodiment, an apparatus is disclosed. The apparatus includes an endoscopic clip placement tool and one or more marking clips attached to a specimen mass by the clip placement tool to mark a margin and orientation of the specimen mass. A specimen marking clip is also provided that is adapted to selectively attach to tissue inside of a patient and corresponding tissue that has been excised from the patient for analysis. Sutures may be associated with the clips to help ensure correct in vivo and ex vivo sample orientation. In vivo clips may remain in the patient's body if necessary.

Disclosed are methods for producing a population of autologous engineered cells to be reinfused into a subject comprising extracorporeally transfecting an internal payload into a population of target cells from a sample obtained from the subject by incubating the population of cells with a lipid nanoparticle (LNP) and / or targeting lipid nanoparticle (tLNP) in an incubation component of an extracorporeal system for no more than 6 hours.

Ionizable cationic lipids, methods for synthesizing the same, intermediates useful in synthesis of the ionizable cationic lipids and methods of synthesizing the intermediates are disclosed. The ionizable cationic lipids are useful as a component of lipid nanoparticles (LNP), which in turn can be used for the delivery of nucleic acids into cells in vivo or ex vivo. LNP compositions are also disclosed, including LNP comprising a functionalized lipid to enable conjugation of a binding moiety, and targeted LNP (tLNP), that is an LNP in which a binding moiety has been conjugated to the functionalized lipid and can serve as a targeting moiety to direct the tLNP to a desired tissue or cell type.

The invention discloses a method for obtaining leymus chinensis mutants by using heavy ion beams to irradiate seed calluses, relates to the technical field of leymus chinensis breeding biology, and solves the three-low problems of low heading rate, low maturing rate and low germination rate of leymus chinensis seeds. After the leymus chinensis callus is subjected to induction culture, selecting faint yellow granular callus obtained by induction culture, and transferring the faint yellow granular callus into a culture dish filled with an induction culture medium for subculture; carrying out irradiation treatment by using a 5Gy carbon ion beam, transferring the callus subjected to irradiation treatment into a sterile culture bottle filled with a differential culture medium, and culturing to obtain adventitious buds; cutting off callus adhered to the base under a sterile condition, and inserting into a rooting culture medium for rooting culture after a rooting point is exposed; and after obvious root systems grow out, carrying out potting culture to obtain the leymus chinensis mutant. According to the invention, new genetic variation can be created, early in-vitro selection is realized, more effective mutants are obtained as new germplasm resources of leymus chinensis, and the breeding process of leymus chinensis is accelerated.

The present invention uses ex vivo human airway cultures to assess the human transmissibility and replication competence of influenza and coronavirus strains. By comparing pandemic influenza A subtype H1N1 and highly pathogenic avian influenza H5N1 as reference strains, the transmissibility risk of various viruses was evaluated and categorized. Additionally, an in vitro model evaluated virus-induced impairment of alveolar fluid clearance (AFC) as an indicator of disease severity. The study revealed correlations between bronchus viral replication, human transmission, AFC impairment, and clinical disease severity across different influenza and coronavirus strains.

The present invention relates to a method for the ex vivo molecular diagnosis of gastrointestinal stromal tumors, comprising the steps of: providing at least one isolated tissue sample comprising at least one nucleic acid and the subsequent extraction of the at least one nucleic acid. The method according to the present invention further comprises a step of quantifying the at least one previously isolated nucleic acid. Furthermore, the method according to the present invention comprises preparing at least one amplicon library of said previously isolated nucleic acid, which comprises sequences obtained from the FGFR1, NF1, SDHA, SDHB, SDHC and SDHD genes. This step is followed by the step of purifying the at least one amplicon library and the step of simultaneously sequencing the amplicons themselves. The present invention also relates to a multi-gene panel for ex vivo molecular diagnosis of gastrointestinal stromal tumors and a kit comprising such a panel.

The present invention relates to a modified stem cell and an ex vivo method for preparing defined mature cells from genetic programming of said modified stem cell which can be carried out in absence of certain growth factors, as well as a kit allowing to transform stem cells, and the different uses of the mature cells, for example for in vitro drug screening and toxicology or as cultivated meat. The genetic programming involves a molecular switch between two mutually exclusive programs of stem cell expansion and differentiation. The invention may be used for stem cells or other progenitors cells of any type, from any eukaryotic organism, but finds particular application in pluripotent stem cells from humans and livestock animals.