872 results about "Ex vivo" patented technology

Provided are ex vivo systems and methods of predicting the response of a drug or other agent on a tissue. In some embodiments, the systems and methods comprise cutting a tissue into tissue fragments, adding a drug or other agent to the tissue fragments based on an estimated tumor content, and performing an ex vivo measurement on the tissue fragments.

Ionizable cationic lipids, methods for synthesizing the same, intermediates useful in synthesis of the ionizable cationic lipids and methods of synthesizing the intermediates are disclosed. The ionizable cationic lipids are useful as a component of lipid nanoparticles (LNP), which in turn can be used for the delivery of nucleic acids into cells in vivo or ex vivo. LNP compositions are also disclosed, including LNP comprising a functionalized lipid to enable conjugation of a binding moiety, and targeted LNP (tLNP), that is an LNP in which a binding moiety has been conjugated to the functionalized lipid and can serve as a targeting moiety to direct the tLNP to a desired tissue or cell type.

The invention discloses a biological in-vitro sample aging quality control detection method, and relates to the technical field of biological samples, and the method specifically comprises the following steps: screening metabolic markers based on biological physiological characteristics, and constructing an in-vitro quality attenuation model; collecting and preprocessing sample data; detecting the concentration of the metabolic marker through detection equipment; inputting marker concentration data into the model for dynamic comparison; and outputting aging classification, and guiding experiment scheduling operation. According to the method, effective use time efficiency is adopted for rapid judgment, the problem that experimental data is influenced by metabolic degradation of a sample after in-vitro is solved, time efficiency grading can be completed in a short time through multi-marker dynamic comparison and a machine learning model, the method is remarkably superior to a traditional method, and by combining a micro-fluidic detection device and an LSTM model, the detection accuracy is greatly improved. According to the method, the samples can be scheduled to proper experiment operation according to the effective time limit of the samples, waste of the samples is avoided, and after failure samples are detected, a user can be reminded to collect the samples again, so that the experiment process is prevented from being delayed.

Provided herein are systems, compositions, and methods for generating immunogenic peptides or epitopes from tumor associated antigens (e.g., in vivo or ex vivo). Polynucleotides (e.g., genes) encoding the tumor associated antigens may be edited at selected target sites by nucleobase editors comprising a catalytically-inactive Cas9 and a cytosine deaminase, leading to the expression of heteroclitic or cryptic peptides that are more immunogenic than the native peptide derived from the tumor associated antigens. The heteroclitic or cryptic peptide elicit strong tumor-specific immune response (e.g., T-cell response or B-cell response), which inhibits tumor growth and metastasis.

Disclosed are methods, compounds, and compositions useful for increasing autophagy and promoting longevity. The methods, compounds, and compositions relate to urolithins and urolithin precursors and use thereof. Certain urolithins are represented by Formula I, while certain urolithin precursors are represented by Formula IV. The urolithin may be urolithin A, urolithin B, urolithin C, or urolithin D. The urolithin precursor may be ellagic acid or an ellagitannin. The methods include in vivo, ex vivo, and in vitro uses of the compounds and compositions.

Anti-CD90 antibodies, binding fragments, and uses thereof are described. The provided antibodies and binding fragments can be used to isolate CD90 cells or to target such cells ex vivo or in vivo for a research, a diagnostic, or a therapeutic purpose. Anti-CD90 antibodies and binding fragments thereof can also be used in engineered formats to create multi-domain binding molecules, antibody conjugates, recombinant receptors, or within a recombinant protein to pseudotype viral vectors.

The disclosure provides conjugates comprising a targeting moiety a lipid nanoparticle (LNP) encapsulating a therapeutic agent (e.g., payload) for delivery to immune cells. The conjugates can be delivered to immune cells ex vivo or formulated in a pharmaceutical composition and can be directly administered to a subject in need of (i.e., via in vivo administration).

The invention belongs to the field of biological medicine, and provides a protection liquid capable of preserving in-vitro animal organs or in-vitro animal tissues at normal temperature as well as a preparation method and application of the protection liquid, and the protection liquid comprises a solution containing a hemoglobin oxygen carrier or a hemoglobin carrier, an osmotic pressure regulating substance and the like. The protective liquid provided by the invention can be used for preserving or perfusing broken limbs, isolated animal organs or isolated animal tissues at normal temperature, and can also be used as a culture liquid of artificial organs and artificial tissues.

Provided are variant Cas12f4 polypeptides, fusion proteins containing variant Cas12f4 polypeptides, nucleic acids encoding variant Cas12f4 polypeptides and fusion proteins, compositions and methods related to those variant Cas12f4 polypeptides and fusion proteins, and modified host cells comprising the variant Cas12f4 polypeptides and / or encoding nucleic acids. The variant Cas12f4 polypeptides disclosed herein exhibit improved functionality, stability, and / or other desirable properties and may be used in combination with guide Cas12f4 RNAs that can be used in a variety of applications ex vivo, in vitro, and in vivo.

The invention relates to the technical field of agricultural breeding, in particular to a bamboo reed saline-alkaline resistant screening and stress-resistant tissue culture seedling raising method which comprises the following steps: step 1, pretreatment; step 2, dynamic salt stress screening: placing the seed stems in a mixed salt-alkali solution with gradient increasing, culturing, measuring the bud length and root length growth rate of axillary buds of the seed stems, fitting a salt concentration-growth rate curve, and extracting a semi-inhibitory concentration IC50 and a maximum tolerance concentration MTC; step 3, salt tolerance grade classification; 4, carrying out stress-resistant tissue culture seedling culture: carrying out in-vitro culture on the axillary bud explant of the high-salt-resistant variety in a culture medium, and inducing clumpy buds and rooting in combination with a salt-resistant inducer; 5, domestication and transplanting: performing gradual salt stress domestication on the tissue culture seedlings, transplanting the tissue culture seedlings into a saline-alkaline environment, and monitoring the adaptability of the tissue culture seedlings in the saline-alkaline environment. According to the method disclosed by the invention, the efficient cultivation system of the suitable-to-grow bamboo reed seedlings in the saline-alkali soil is established by fusing dynamic stress response analysis and stress resistance phenotype directional strengthening technologies.

The invention discloses a method for controlling left and right heart compaction degree before extracorporeal circulation operation, which comprises the following steps of: installing a peristaltic pump at a left and right heart inlet of an extracorporeal circulation device in an in-vitro environment; a liquid inlet of the peristaltic pump is connected with a liquid inlet pump, and a liquid outlet of the peristaltic pump is connected with a liquid outlet pump; and finally, a first pressure measuring structure and a second pressure measuring structure are arranged on the sides, away from the peristaltic pump, of the liquid inlet pump and the liquid outlet pump correspondingly, and the pressure of the liquid inlet pump and the pressure of the liquid outlet pump are adjusted to be-180 mmHg. The negative pressure of the liquid inlet pump is adjusted through the first pressure measuring structure, and the negative pressure of the liquid outlet pump is adjusted through the second pressure measuring structure, so that the purpose of quantifying the adjusting mode is achieved, the adjusting process for controlling the left and right heart compression degree before the extracorporeal circulation operation is visualized, the adjusting result is converted into the specific negative pressure values of the liquid inlet pump and the liquid outlet pump, and the accuracy of the operation is improved. The pressing degree reference for adjusting the left center and the right center is numeralized and standardized, the adjusting difference is avoided, experience and feeling are not needed, and the adjusting mode is fast and high in accuracy.

Disclosed are methods, compounds, and compositions useful for increasing autophagy and promoting longevity. The methods, compounds, and compositions relate to urolithins and urolithin precursors and use thereof. Certain urolithins are represented by Formula I, while certain urolithin precursors are represented by Formula IV. The urolithin may be urolithin A, urolithin B, urolithin C, or urolithin D. The urolithin precursor may be ellagic acid or an ellagitannin. The methods include in vivo, ex vivo, and in vitro uses of the compounds and compositions.

The present invention provides preparations of MSCs with important therapeutic potential. The MSC cells are non-primary cells with an antigen profile comprising less than about 1.25% CD45+ cells (or less than about 0.75% CD45+), at least about 95% CD105+ cells, and at least about 95% CD166+ cells. Optionally, MSCs of the present preparations are isogenic and can be expanded ex vivo and cryopreserved and thawed, yet maintain a stable and uniform phenotype. Methods are taught here of expanding these MSCs to produce a clinical scale therapeutic preparations and medical uses thereof.

According to one embodiment, an apparatus is disclosed. The apparatus includes an endoscopic clip placement tool and one or more marking clips attached to a specimen mass by the clip placement tool to mark a margin and orientation of the specimen mass. A specimen marking clip is also provided that is adapted to selectively attach to tissue inside of a patient and corresponding tissue that has been excised from the patient for analysis. Sutures may be associated with the clips to help ensure correct in vivo and ex vivo sample orientation. In vivo clips may remain in the patient's body if necessary.

Disclosed are methods for producing a population of autologous engineered cells to be reinfused into a subject comprising extracorporeally transfecting an internal payload into a population of target cells from a sample obtained from the subject by incubating the population of cells with a lipid nanoparticle (LNP) and / or targeting lipid nanoparticle (tLNP) in an incubation component of an extracorporeal system for no more than 6 hours.

Ionizable cationic lipids, methods for synthesizing the same, intermediates useful in synthesis of the ionizable cationic lipids and methods of synthesizing the intermediates are disclosed. The ionizable cationic lipids are useful as a component of lipid nanoparticles (LNP), which in turn can be used for the delivery of nucleic acids into cells in vivo or ex vivo. LNP compositions are also disclosed, including LNP comprising a functionalized lipid to enable conjugation of a binding moiety, and targeted LNP (tLNP), that is an LNP in which a binding moiety has been conjugated to the functionalized lipid and can serve as a targeting moiety to direct the tLNP to a desired tissue or cell type.

The invention discloses a method for obtaining leymus chinensis mutants by using heavy ion beams to irradiate seed calluses, relates to the technical field of leymus chinensis breeding biology, and solves the three-low problems of low heading rate, low maturing rate and low germination rate of leymus chinensis seeds. After the leymus chinensis callus is subjected to induction culture, selecting faint yellow granular callus obtained by induction culture, and transferring the faint yellow granular callus into a culture dish filled with an induction culture medium for subculture; carrying out irradiation treatment by using a 5Gy carbon ion beam, transferring the callus subjected to irradiation treatment into a sterile culture bottle filled with a differential culture medium, and culturing to obtain adventitious buds; cutting off callus adhered to the base under a sterile condition, and inserting into a rooting culture medium for rooting culture after a rooting point is exposed; and after obvious root systems grow out, carrying out potting culture to obtain the leymus chinensis mutant. According to the invention, new genetic variation can be created, early in-vitro selection is realized, more effective mutants are obtained as new germplasm resources of leymus chinensis, and the breeding process of leymus chinensis is accelerated.

The present invention uses ex vivo human airway cultures to assess the human transmissibility and replication competence of influenza and coronavirus strains. By comparing pandemic influenza A subtype H1N1 and highly pathogenic avian influenza H5N1 as reference strains, the transmissibility risk of various viruses was evaluated and categorized. Additionally, an in vitro model evaluated virus-induced impairment of alveolar fluid clearance (AFC) as an indicator of disease severity. The study revealed correlations between bronchus viral replication, human transmission, AFC impairment, and clinical disease severity across different influenza and coronavirus strains.

The present invention relates to a method for the ex vivo molecular diagnosis of gastrointestinal stromal tumors, comprising the steps of: providing at least one isolated tissue sample comprising at least one nucleic acid and the subsequent extraction of the at least one nucleic acid. The method according to the present invention further comprises a step of quantifying the at least one previously isolated nucleic acid. Furthermore, the method according to the present invention comprises preparing at least one amplicon library of said previously isolated nucleic acid, which comprises sequences obtained from the FGFR1, NF1, SDHA, SDHB, SDHC and SDHD genes. This step is followed by the step of purifying the at least one amplicon library and the step of simultaneously sequencing the amplicons themselves. The present invention also relates to a multi-gene panel for ex vivo molecular diagnosis of gastrointestinal stromal tumors and a kit comprising such a panel.

The present invention relates to a modified stem cell and an ex vivo method for preparing defined mature cells from genetic programming of said modified stem cell which can be carried out in absence of certain growth factors, as well as a kit allowing to transform stem cells, and the different uses of the mature cells, for example for in vitro drug screening and toxicology or as cultivated meat. The genetic programming involves a molecular switch between two mutually exclusive programs of stem cell expansion and differentiation. The invention may be used for stem cells or other progenitors cells of any type, from any eukaryotic organism, but finds particular application in pluripotent stem cells from humans and livestock animals.

The invention provides a method and system for calculating the utilization rate of soil nitrogen under the action of arbuscular mycorrhiza, and relates to the technical field of calculation of the utilization rate of soil nitrogen under the action of arbuscular mycorrhiza. Soil nitrogen absorption amount data of an experimental group and a control group are collected, meanwhile, the total amount of externally input nitrogen is recorded, and related photosynthetic parameters of plants are measured; in addition, through an in-vitro mycelium culture experiment, mycelium nitrogen absorption rates under different nitrogen concentrations are measured, and a semi-saturation constant and a nitrogen effectiveness index are calculated, so that a carbon-nitrogen coupling factor is calculated, morphological parameters of mycelia are collected, and a relation equation of a pipe diameter and a transport rate is established; calculating the total flux of the hypha network, calculating the original nitrogen utilization rate in combination with the nitrogen absorption amount difference value of the experimental group and the control group and externally input nitrogen data, and introducing a transportation continuity coefficient for dynamic correction to finally obtain the soil nitrogen utilization rate of the target area.

The invention relates to a preparation method and application of a metal ion type lipid nanoparticle delivery system for regulating mRNA transfection. The method comprises the following steps: (1) mixing mRNA and metal ions in a solvent for 5-15 minutes to obtain a mixture solution (water phase); and (2) blowing, beating and mixing the nanoparticle-containing solvent obtained in the step (1) and a lipid solvent, and wrapping the mixture in the water phase with lipid nanoparticles to obtain the metal ion type lipid nanoparticle delivery system for regulating mRNA transfection. The preparation method has the characteristics of mild preparation conditions and simple preparation steps. According to the application, the organ specific transfection capacity of a lipid nanoparticle delivery system containing different metal ions is verified through mouse experiments. An in-vitro imaging result shows that the system has an enhancement or inhibition effect on mRNA transfection in different organs. The discovery provides a reference basis for metal ion selection for clinically and accurately regulating mRNA in-vivo transfection, and has an important guiding value.

The invention relates to the technical field of plant genetic engineering, in particular to a ginkgo GbGRF2 gene and application of a GbGRF2-GbGIF2 fusion gene of the ginkgo GbGRF2 gene in plant tissue culture. According to the invention, the GbGRF2 gene coding region full length of the GRF gene family of gingko is successfully cloned from gingko, and the gene presents a specific expression mode in the regeneration process of gingko callus; the overexpression of the fusion gene of GbGRF2 and GbGRF2-GbGIF2 can significantly promote the regeneration efficiency of adventitious buds in the in-vitro culture process of plants; the gene provided by the invention has important theoretical significance and application potential in a plant tissue regeneration regulation network, and plays a key role in improving in-vitro regeneration efficiency of gymnosperm.

The present invention relates generally to immunization and immunotherapy for the treatment or prevention of HIV. In particular, the methods include in vivo and / or ex vivo enrichment of HIV-specific CD4+ T cells.

Modular mucosal tissue bioreactors combined with bioartificial mucus provide a high-fidelity, medium-throughput platform for evaluating CF treatments and modeling host-pathogen biology, a stepping stone towards better options for HEMT-nonresponsive patients. Whole lung ex vivo Cystic Fibrosis model system allows for investigation of candidate CF therapies.

The invention relates to a construction method of a thyroid-related eye disease animal model, and belongs to the technical field of biology. The invention provides a thyroid-associated ophthalmopathy animal model construction method, which comprises: (1) separating and purifying in vitro peripheral blood collected from a thyroid-associated ophthalmopathy patient to obtain an exosome; and (2) injecting the exosome into a to-be-modeled animal to prepare the thyroid-associated ophthalmopathy animal model. According to the invention, exosomes are extracted from in-vitro peripheral blood of patients with thyroid-associated ophthalmopathy and are injected into animals, so that the thyroid-associated ophthalmopathy animal model with a plurality of pathological characteristics such as orbital tissue inflammatory response, fibrosis, orbital lesion, thyroid dysfunction and the like is successfully constructed; the method can be widely applied to pathological mechanism research of thyroid-related eye diseases and screening and evaluation of thyroid-related eye disease treatment drugs.

Ionizable cationic lipids, methods for synthesizing the same, intermediates useful in synthesis of the ionizable cationic lipids, and methods of synthesizing the intermediates are disclosed. The ionizable cationic lipids are useful as a component of lipid nanoparticles (LNP), which in turn can be used for delivering nucleic acids into cells in vivo or ex vivo. LNP compositions are also disclosed, including LNP comprising a functionalized lipid to enable conjugation of a binding moiety, and targeted LNP (tLNP), that is an LNP in which a binding moiety has been conjugated to the functionalized lipid and can serve as a targeting moiety to direct the tLNP to a desired tissue or cell type.

To provide non-viral DNA vectors for expressing FVIII therapeutic agents and uses thereof.SOLUTION: The application describes a ceDNA vector having a linear and continuous structure for the delivery and expression of a transgene. The ceDNA vector comprises an expression cassette franked by two ITR sequences, where the expression cassette encodes a transgene encoding FVIII protein. Some ceDNA vectors further comprise a cis-regulatory element including a regulatory switch. Further provided herein are methods and cell lines for reliable gene expression of FVIII protein in vitro, ex vivo and in vivo using the ceDNA vectors.SELECTED DRAWING: None

The present application provides compositions and methods for determining hepatocellular carcinoma (HCC) in a subject. The method comprises contacting a body fluid with a molecule comprising a reporter thereof and the reported is cleaved by an agent in the body fluid. Wherein, the subject has or is suspected of having cirrhosis of a liver.

The invention relates to the field of immunotherapy, more in particular to a composition for use in the treatment of cancer. The invention also relates to a composition obtainable by such a method, such as a pharmaceutical composition. More in particular, the invention relates to an ex vivo method for obtaining a composition suitable for the treatment of cancer in a subject, comprising the steps of providing primary tumor cells derived from the subject, and ex vivo contacting the tumor cells with an inhibitor of a bromodomain and extra-terminal domain family member (BET inhibitor).