12 results about "Calpain" patented technology

The invention belongs to the field of neurobiology and drug research and development, and discloses application of NR3A as a protein target in screening or preparing drugs for preventing and / or treating depression-like behaviors. According to the invention, the expression of NR3A in hippocampus is enhanced by stereotactic injection of adeno-associated virus containing Grin3a gene. A chronic constraint stress (CRS) is adopted to induce a depressive mouse, and a sucrose preference test (SPT), a forced swimming test (FST) and an open field test (OFT) are adopted for identification. The expression of proteins such as NR3A, calpain 2 (Calpain2), neuron nitric oxide synthase (nNOS), 3-nitrotyrosine (3-NT), an NLRP3 inflammation complex and the like is detected by WesternBlot; and performing DHE staining to detect reactive oxygen species (ROS). By enhancing hippocampus NR3A expression, depression-like behaviors induced by CRS can be improved, signal cascade activated by calcium is inhibited, ROS generation and 3-NT expression are reduced, and neuroinflammation driven by NLRP3 inflammasomes is inhibited. The NR3A participates in depression-like behaviors induced by CRS, and the mechanism of the NR3A is related to calcium homeostasis regulation, ROS generation and inflammatory response driven by NLRP3 inflammasomes.

Disclosed herein are pharmaceutical formulations comprising antisense oligonucleotides, or salts thereof, that reduce expression of CAPN2, which encodes calpain-2.

The present invention addresses the problem of providing a protective agent for retinal pigment epithelial cells, which is useful for the prevention or treatment of age-related macular degeneration. A protective agent for retinal pigment epithelial cells according to the present invention, which is a solution for the problem, contains, as an active ingredient, a peptide in which a protein transduction domain is attached to the N-terminus of a peptide having an inhibitory effect on mitochondrial calpains. A specific example of the peptide having an inhibitory effect on mitochondrial calpains is a peptide comprising the amino acid sequence (PDALKSRTJR) represented by SEQ ID NO: 1 (wherein J represents L or I). A specific example of the protein transduction domain is a peptide comprising an amino acid sequence that is at least a part of the amino acid sequence for the transduction domain of HIV-1 Tat.

The invention relates to an anthrax protein nanopore system and application thereof in protein sequencing, and belongs to the technical field of nanopore detection. The invention aims to solve the problems of low flux, insufficient accuracy and limited nanopore resolution in the existing protein sequencing technology. According to the invention, an anthrax protein PA63 heptamer nanopore electrochemical detection device is constructed, an asymmetric pH buffer system is adopted, Trypsin, Calpain-1, Pancreative Elatase, CarboxyceptidaseA enzyme and the like are added step by step for polypeptide enzyme digestion, voltage is applied to drive amino acid to pass through nanopores, current signals are collected, amplitude, retardation time and frequency characteristics are analyzed, and polypeptide amino acid sequence detection is realized. The nanopore is small in opening current (fA level) and high in resolution ratio, detection accuracy and sensitivity are enhanced by combining multi-enzyme step-by-step cutting and carving, and a direct and efficient sequencing means is provided for protein function research.

The invention discloses a beta-lactoglobulin nanofiber as an adjuvant for oral delivery of therapeutic polypeptide drugs and application of the beta-lactoglobulin nanofiber, and belongs to the technical field of drug delivery adjuvants, the nanofiber is formed by heat treatment of BLG under an acidic condition, and has good gastrointestinal tract stability and severe environment tolerance; in the oral administration process, the nanofibers can temporarily induce the activity of calcium-dependent calpain to be improved, so that the intestinal epithelial permeability is enhanced, and the absorption of polypeptide drugs is facilitated; the rapid recovery of the intestinal barrier function can also be promoted. Insulin is loaded in an enteric capsule coated with 3% of sodium alginate, and by utilizing the BLG nanofiber mediated intestinal barrier regulation effect, compared with subcutaneous injection, the oral administration of insulin shows that the area under a blood glucose curve (AUC) is obviously reduced in a mouse model, and the blood glucose AUC is reduced by about 20.8%. The nanofiber is high in safety and meets the clinical large-scale production requirement.

The invention discloses application of SBDP145 and calpain in preparation of a systemic lupus erythematosus diagnosis product, and belongs to the technical field of disease detection. Specifically, through comparison of transcriptome data of a healthy control and a systemic lupus erythematosus (SLE) patient from a database, it is found that calpain has a significant difference in the two groups of data; and further comparing the calpain and the cracking product SBDP145 of the substrate of the calpain in the serum of the healthy control and the SLE patient, and finding that the calpain and the SBDP145 are up-regulated in the patient group, so that the calpain and the SBDP145 are used as candidate diagnostic markers. Then, an actual serum sample is adopted to explore the expression difference of calpain in healthy control and patients, the disease correlation between SBDP145 and calpain and systemic lupus erythematosus and the like, and the feasibility of the marker in SLE diagnosis and detection is verified. The invention provides a new SLE detection strategy, which is expected to overcome the defects of the traditional auxiliary diagnosis mode, and provides a new choice for clinical SLE diagnosis, illness monitoring and prognosis evaluation.

The present invention relates to a composition and a method capable of predicting sulfonylurea dependence in diabetic patients by identifying the variants of: rs777077261(C>A) of KCNB2(Potassium Voltage-Gated Channel Subfamily B Member 2); rs202112906(C>T) of GHSR(Growth hormone secretagogue receptor); rs28944173(T>C) of NOS2(Nitric oxide synthase 2); rs3792268(C>A) of CAPN10(Calpain 10); rs199670165(C>A), rs17848945(C>T), rs755372392(G>A), rs200840955(G>A), rs200842352(C>A) or rs45489599(C>T) of FASN(Fatty acid synthase); rs185358392(G>A), rs769120237(G>A), rs201884071(G>A) or rs766766661(C>T) of KCNH6(Potassium Voltage-Gated Channel Subfamily H Member 6); rs14847401(G>A), rs37551700(G>A) or rs774656238(A>G) of DIS3L2(DIS3 like 3'-5' exoribonuclease 2); or rs74315349(G>A) or rs150546920(C>G) of NR0B2(Nuclear Receptor Subfamily 0 Group B Member 2).

Provided herein are methods and compositions for treating at least one symptom of ALS, slowing ALS disease progression, or reducing the deterioration of one or more bodily functions affected by ALS in a subject. The methods can include administering to the subject an antisense oligonucleotide that targets calpain-2, which is encoded by the CAPN2 gene.

The application discloses a processing method of fried white fish, and relates to the field of aquatic product processing, and comprises the following steps: S1, obtaining fish fillets with skin; S2, gradient pickling the fish fillets in pickling liquid; S3, rinsing the surface of the fish fillets with clean water, draining, and then carrying out dehydration treatment; S4, two-stage frying of the fish fillets after the dehydration treatment is carried out by taking vegetable oil as a medium; and S5, cooling the fried fish fillets to room temperature, vacuum packaging, and then carrying out ultrahigh pressure treatment. According to the method, endogenous calpain in fish meat is selectively activated, muscle fiber protein is moderately degraded, the pickling time is shortened, and a continuous natural protein film is formed. In the process of gradient temperature frying, the protein film forms a water-locking barrier and is converted into a crisp outer shell, and the internal fish meat is cooked in a nearly micro-steaming environment under the steam blocking effect of the film, so that water is retained, and finally, a texture level with a crisp outer layer and a tender internal layer is formed.

Provided herein is a mutant JP2 protein comprising a deletion of a calpain cleavage site and methods of using the same.

This disclosure provides, in particular, oligonucleotides targeting calpain 2 that are modified at the 2'-position of sugar residues and compositions thereof. In some embodiments, this disclosure provides methods of preventing or treating a variety of conditions, disorders, or diseases including neurodegenerative conditions.

The present disclosure provides, among other things, oligonucleotides targeting calpain-2 modified at the 2 '-position of a sugar residue and compositions thereof. In some embodiments, the disclosure provides methods for the prevention and treatment of various conditions, disorders, or diseases, including neurodegenerative conditions.