59 results about "Calpain" patented technology

The present invention relates to novel fluorescent dyes, novel fluorogenic and fluorescent reporter molecules and new enzyme assay processes that can be used to detect the activity of caspases and other enzymes involved in apoptosis in whole cells, cell lines and tissue samples derived from any living organism or organ. The reporter molecules and assay processes can be used in drug screening procedures to identify compounds which act as inhibitors or inducers of the caspase cascade in whole cells or tissues. The reagents and assays described herein are also useful for determining the chemosensitivity of human cancer cells to treatment with chemotherapeutic drugs. The present invention also relates to novel fluorogenic and fluorescent reporter molecules and new enzyme assay processes that can be used to detect the activity of type 2 methionine aminopeptidase, dipeptidyl peptidase IV, calpain, aminopeptidase, HIV protease, adenovirus protease, HSV-1 protease, HCMV protease and HCV protease.

The invention discloses a determining method for the phosphorylation level of calpain. The determining method includes the steps that 1, the protein concentration of a protein sample containing calpain is adjusted to 5.83-12.5 micrograms/microliter, a phosphorylated antibody is added into the protein sample, the mass ratio of the phosphorylated antibody to the total reacting weight of protein in the protein sample containing calpain is 1 to 1750-2500, then the phophorylated antibody and the protein sample are subjected to vibration hatching at 4 DEG C to form an immune complex, and then through a protein immune precipitation method, a phosphorylated calpain sample is obtained through gathering; 2, through a protein western blot method, the phosphorylation level of the phosphorylated calpain sample obtained in the step1 is detected. Through the protein immune precipitation technology and the western blot technology, phosphorylated calpain in post-slaughter muscles can be detected, the number of needed muscle samples is small, the operation process is simple, repeatability is good, stripes obtained through detection are clear, and the test method is small in potential hurt to the human body.

The invention discloses the stability application of Calpain or the excitant or antagonist of Calpain in preparing AchR aggregate that regulates neuromuscular junction. The invention also discloses a method for screening the agent that regulates the stability of the AchR aggregate of neuromuscular junction by using the Calpain.

The invention discloses a processing method of raw material meat for small crispy meat, and belongs to the technical field of meat processing. The processing method comprises the following steps of S1: rinsing live pigs, performing slaughtering so as to obtain bodies, and performing spraying and disinfecting on the bodies; S2: performing heat bone removing, fat removing and pig skin removing on pig hind legs obtained in the step S1, and injecting a calcium chloride solution of which the concentration is 145-155ppm and the mass is 0.2%-0.5% of that of lean meat to the obtained lean meat; and S3: cooling the lean meat processed in step S1 under minus 40 to minus 35 DEG C for 30-40 minutes, so that thin ice is generated on the surfaces of the lean meat, and then putting the cooled lean meat in a precooling storage of which the temperature is 2-4 DEG C and the humidity is 85-95% for 18-24 hours. According to the processing method disclosed by the invention, the calcium chloride solution isinjected to the hot fresh pig hind legs, so that the activity of calpains in muscles is improved, and the mature of the meat of the pig hind legs is accelerated. The invention further provides a method of preparing the small crispy meat by using the raw material meat. The invention further provides the method for preparing the small crispy meat by using the raw material meat, and the method can realize industrial production of the small crispy meat.

This invention relates to a method of calcium protease restrain albumen gene 475 place SNP mark filtration of pig, it belongs to domestic animal molecular biology technique field. The procedures includes that distilling of pig gene group RNA, reverse transcription PCR amplification of calcium protease restrain albumen gene, detecting of SNP mark and its correlative analysis to pork tenderness. The feature is that new SNP mark of pig calcium protease restrain albumen gene is separated and determined, one SNP mark propitious to pork is filtrated (475 place C to T), prime used to amplify the place us designed, and kit used for this method is designed. This invention used the correlative characters between the SNP of 475 place in encoding area of calcium protease restrain albumen gene and pork tenderness to provides a new molecule mark method for pig mark assistant selection and matching.

The invention discloses a preserved meat product preservation method. The method comprises the following steps: (1) screening a culture for fermenting a preserved meat product, and establishing preserved meat fermentation conditions; (2) determining the types and the amount of enzymes and enzymolysis conditions, adding papain, pancrelipase, calpains, pronase and other enzyme preparations into the salting process by taking the texture, flavor, color and the like as indexes, and determining the enzyme addition amount, the enzymolysis time, the enzymolysis temperature and other enzymolysis method conditions; (3) analyzing the acid value and change of peroxide value in the preserved meat production process, and selecting an antioxidant; and (4) preparing an edible film, establishing a coating technology, wherein microbes, the acid value, the peroxide value and the TBA value are used as indexes, different composite edible film materials such as chitosan, modified starch and soy isolate protein are used, and nisin or nisin are added, and performing antiseptic validity treatment on the product by adopting dipping, spraying and other different coating technologies. The method is easy to operate and obvious in effect.

This invention relates to a method of calcium protease restrain albumen gene 1836 place SNP mark filtration of pig, it belongs to domestic animal molecular biology technique field. The procedures includes that distilling of pig gene group RNA, reverse transcription PCR amplification of calcium protease restrain albumen gene, detecting of SNP mark and its correlative analysis to pork tenderness. The feature is that new SNP mark of pig calcium protease restrain albumen gene is separated and determined, one SNP mark propitious to pork is filtrated (1836 place C to T), prime used to amplify the place us designed, and kit used for this method is designed. This invention used the correlative characters between the SNP of 1836 place in encoding area of calcium protease restrain albumen gene and pork tenderness to provides a new molecule mark method for pig mark assistant selection and matching.

A composition comprising a peptide or a peptidomimetic thereof that inhibits the C-terminal domain truncation of mGluR1α by calpain, wherein the peptide is 10-30 amino acids in length and contains a sequence that is at least 70% homologous to VIKPLTKSYQGSGK. Also disclosed are methods of detecting the C-terminal domain truncation of mGluR1α by calpain, methods of inhibiting the C-terminal domain truncation of mGluR1α in a neuron, and methods of identifying a compound that inhibits the C-terminal domain truncation of mGluR1α.