41 results about "Lateolabrax" patented technology

The invention, which belongs to the technical field of the aquatic livestock culturing, particularly relates to a four-level culture method of a lateolabrax japonicus pond. First-level cultivation, second-level cultivation, third-level cultivation, and adult fish cultivation are carried out on lateolabrax japonicus in a pond successively. A third-level cultivation pond is encircled by a net and a pond base in a pond; a second-level cultivation pond is encircled by a net and a pond base in the third-level cultivation pond; and a first-level cultivation pond is encircled by a net and a pond base in the second-level cultivation pond. According to the invention, with optimization of the culturing technology in a graded culturing mode, ways containing the culturing means like ecological mixed breeding are improved; the seedlings can be domesticated to eat food in a centralized mode and thus seedlings with uniform sizes can be cultivated; the yield of the fries can be improved; the average acre yield of the lateolabrax japonicus can be increased; and the comprehensive benefits of the lateolabrax japonicus culturing can be improved.

The invention discloses a method for north-south hybridization and reproductive regulation breeding of Lateolabrax, which relates to the biotechnology breeding of Lateolabrax. The method comprises the following steps of: (1) taking a standby parent fish bred from the northern Lateolabrax seed as the male parent and a standby parent fish bred from the local wild Lateolabrax seed as the female parent; (2) breeding the male and the female parents to about 1500g to be used as the parent fishes; (3) artificially inducing the Lateolabrax through a reproductive regulation: controlling the time and the number of hatching of the Lateolabrax parent fishes by means of temperature, photoperiod and hormone injection, controlling the illumination time to 8h, reducing the temperature by 0.3 DEG C every day via a water cooler, and injecting CRH-A3 to the dorsal muscles of the parent fishes after anesthesia with allylguaiacol when the water temperature is reduced to 22 DEG C to induce the parent fishes to spawn, wherein the reproductive regulation induction period of the Lateolabrax is 7 days, and the Lateolabrax can be induced for several periods; (4) breeding the germ cells of the Lateolabrax; and (5) breeding juvenile fishes and breeding the juvenile fishes on a lower row when the sizes of the juvenile fishes reach more than 5cm. The invention has the advantage of breeding the new Lateolabrax seed which has the advantages of high temperature resistance, high growth speed, strong disease resistance and good quality by hybridizing northern and southern Lateolabraxes.

The invention discloses a high-quality compound feed with low nitrogen and phosphorus emission for Lateolabrax, and aims at providing the high-quality compound feed with low nitrogen and phosphorus emission for the Lateolabrax, which has the advantages of balanced nutrition and low nitrogen and phosphorus emission, and can be used for protecting liver and enhancing immunity. The high-quality compound feed comprises the following components in percentage by weight: 38-46% of steamed fish powder, 29-35% of bean pulp, 15-25% of flour, 1-4% of kelp powder, 2-6% of fish oil, 0.5-1.5% of monocalcium phosphate, 0.01-0.04% of protease, 0.01-0.04% of phytase, 0.2-0.4% of galactooligosaccharide, 0.20-0.35% of choline chloride, and the remaining of additives containing composite vitamin, composite minerals, Vc phosphate, salt, mildewcide and antioxidant. The high-quality compound feed can be widely applied to the field of the cultivation of the Lateolabrax.

The invention discloses a pollution-free cultivation method of lateolabrax japonicus. Through selecting a sea area and adopting a flow stop measure, the flow speed in a net cage is controlled within 0.2 meter per second, model selection and setting are carried out on the net cage, a floating type net cage with knotless meshes is adopted, a main harbor road and auxiliary harbor roads are arranged between the net cages, lateolabrax japonicus fry stocking, adult fish feeding, feed and feeding, daily management, disease control, harvesting, transportation and the like are designed, strict monitoring and detection are carried out at each stage during the cultivation period, the growth environment and the growth condition of the lateolabrax japonicus are controlled, the feeding condition of the lateolabrax japonicus is detected, and a garbage collection and processing device is provided, so that the cultivation of the lateolabrax japonicus is pollution-free and non-hazard, and the lateolabrax japonicus cultivated through the cultivation method is totally cultivated in sea water, the cultivated lateolabrax japonicus has the characteristics of bright body color, tender meat quality, delicious taste, rich nutrient and the like, and the pollution-free cultivation of the lateolabrax japonicus is implemented.

The invention provides Lateolabrax japonicus stem cells and the culturing method, which comprises the steps of collecting Lateolabrax japonicus blastula stage cells, embryo stem cells primary culture and subculture, influencing growth of Lateolabrax japonicus embryo stem cells by temperature, culturing embryo stem cells in stationary phase, and identifying the Lateolabrax japonicus embryo stem cell features. The embryo stem cells are cultured with complete medium.

The invention relates to fish feed, in particular to lateolabrax japonicus adult fish compound feed and a preparation method thereof. The lateolabrax japonicus adult fish compound feed comprises, by weight, 20-30 parts of fish meal, 8-14 parts of dried clam worm meal, 8-14 parts of sargassum thunbergii powder, 6-10 parts of bean pulp, 6-10 parts of cottonseed meal, 4-8 parts of cordate houttuynia, 4-6 parts of sweet potato flour, 4-6 parts of almond powder, 2-4 parts of silkworm chrysalises, 2-4 parts of dried carrot powder, 2-4 parts of fish oil, 1-3 parts of dried hawthorn powder, 1-3 parts of shell powder, 1-3 parts of honey, 1-2 parts of olive oil and 1-2 parts of compound vitamins. After the lateolabrax japonicus adult fish compound feed is used for feeding, the feed coefficient of lateolabrax japonicus is low, crude protein content in flesh of the lateolabrax japonicus is high, fat content is low, and flavor amino acid content is high.

The invention relates to a preparation for preventing lateolabrax japonicus enteritis and also relates to a preparation method of the preparation for preventing the lateolabrax japonicus enteritis. The preparation for preventing the lateolabrax japonicus enteritis is prepared from the following components in parts by mass: 27 to 35 parts of pomegranate barks, 22 to 26 parts of fresh hydrocotyle sibthorpioides juice, 21 to 26 parts of hibiscus barks, 21 to 25 parts of Rodgersia pinnata Franch., 20 to 25 parts of creeping oxalis, 19 to 23 parts of dandelions, 18 to 24 parts of aconitum gymnandrum maxim, 17 to 23 parts of houttuynia cordata, 17 to 22 parts of cocklebur fruits, 15 to 23 parts of hawthorn fruits, 14 to 20 parts of peruvian groundcherry herb, 14 to 20 parts of common cnidium fruits, 14 to 20 parts of lotus seeds, 13 to 19 parts of roots of Szechwan raspberry, 12 to 18 parts of Myositis sylvatica, 12 to 18 parts of Chinese galls, 10 to 18 parts of lophatherum gracile, 11 to 17 parts of resurrection lily rhizomes, 10 to 16 parts of blackberry lily and the like. The preparation for preventing the lateolabrax japonicus enteritis, provided by the invention, has a good treatment effect on the lateolabrax japonicus enteritis.

The invention discloses a preparing method of gene, which comprises the following steps: designing merge primer with conservative area of near source specie IL-8 gene CDS sequence; extracting total RNA from spleen of colorful bass; augmenting with PCR method; cloning to total expressing sequence of colorful bass interleukins 8 (IL-8) with RACE technique. This gene lays the foundation for generating mechanism of further probing fish inflammation reaction.

The invention relates to fish feed, in particular to compound feed for Lateolabrax japonicus juveniles and a preparing method thereof. The compound feed comprises, by mass, 10 to 20 parts of chicken powder, 8 to 14 parts of dry clamworm powder, 6 to 12 parts of dry red worm powder, 6 to 10 parts of wheat bran, 6 to 10 parts of cottonseed meal, 4 to 8 parts of dry clam visceral mass, 4 to 8 parts of rapeseed, 4 to 6 parts of heartleaf houttuynia herb, 2 to 4 parts of fish oil, 1 to 3 parts of olive oil, 1 to 3 parts of welsh-onion stalk, 2 to 4 parts of dry carrot meal, 1 to 3 parts of silkworm pupa and 1 to 3 parts of shell powder. The compound feed is provided according to the energy metabolism mode and nutrition needs of the Lateolabrax japonicus juveniles so as to fully replace the fish meal, and the Lateolabrax japonicus juveniles fed with the compound feed has high survival rate, low feed coefficient and specific growth rate.

The invention relates to a feed additive for preventing gill-rot disease of lateolabrax japonicus and also relates to a preparation method thereof. The feed additive consists of the following ingredients in parts by mass: 24-34 parts of watermelon juice, 20-26 parts of gleditsia sinensis juice, 20-26 parts of bitter gourd juice, 19-25 parts of dandelion, 19-25 parts of blackend swallowwort root, 19-25 parts of pulsatilla chiensis, 17-24 parts of acrous calamus, 16-24 parts of morning glory, 17-23 parts of astragalus smicus, 15-24 parts of Eucommia ulmoides, 16-23 parts of radix pseudostellariae, 13-23 parts of Rodgersia pinnata, 8-16 parts of Chenopodium ambrosioides, 18-25 parts of dry nereid powder, 15-23 parts of euphausia superba powder, 9-15 parts of dry yeast powder, 11-18 parts of honey, 12-18 parts of fish oil and the like. The feed additive can effectively prevent the gill-rot disease of the lateolabrax japonicus.

The invention relates to a feed additive for preventing lateolabrax japonicus enteritis and also relates to a preparation method of the feed additive for preventing the lateolabrax japonicus enteritis. The feed additive for preventing the lateolabrax japonicus enteritis is prepared from the following components in parts by mass: 27 to 35 parts of pomegranate barks, 22 to 26 parts of fresh hydrocotyle sibthorpioides juice, 21 to 26 parts of hibiscus barks, 21 to 25 parts of rhizome of paleaceous knotweed, 20 to 25 parts of creeping oxalis, 19 to 23 parts of dandelions, 18 to 24 parts of aconitum gymnandrum maxim, 15 to 23 parts of hawthorn fruits, 14 to 20 parts of peruvian groundcherry herb, 14 to 20 parts of common cnidium fruits, 14 to 20 parts of lotus seeds, 13 to 19 parts of roots of Szechwan raspberry, 12 to 18 parts of myositis, 12 to 18 parts of Chinese galls, 10 to 18 parts of lophatherum gracile, 11 to 17 parts of resurrection lily rhizomes, 10 to 16 parts of blackberry lily and the like. The feed additive for preventing the lateolabrax japonicus enteritis, provided by the invention, can be used for effectively preventing the lateolabrax japonicus enteritis from occurring.

The invention provides a lateolabrax japonicus FGF6A, FGF6B and FGF18 recombinant protein as well as a preparation method and application thereof, the lateolabrax japonicus FGF6A, FGF6B and FGF18 recombinant protein comprises FGF6A, FGF6B and FGF18 mature peptide amino acid sequences and tag peptide fragments connected to N ends of the FGF6A, FGF6B and FGF18 mature peptide amino acid sequences, and the tag peptide fragments comprise group amino acid tag sequences and dissolution promoting tag sequences. The invention also provides a gene for coding the recombinant protein, a vector for expressing the recombinant protein and a recombinant engineering bacterium. The invention further provides a preparation method of the recombinant protein and application of the recombinant protein in breeding of lateolabrax japonicus. Soluble FGF6A, FGF6B and FGF18 recombinant proteins can be obtained through large-scale induced expression of prokaryotic bacteria, and the recombinant proteins have activity and are beneficial to subsequent industrial extraction and purification.

The invention discloses an anti-wave cage breeding method for lateolabrax japonicas and belongs to the technical field of mariculture. The method includes the following steps of 1, aquaculture sea area selection, 2, cage selection, 3, stocking of lateolabrax japonicas flies, 4, feed feeding, 5, breeding management, 6, daily management and disease prevention and treatment. According to the method, through scientific arrangement of the cages, each group of cages are longitudinally arranged in the flowing direction of tidewater, so that blocking of water flow by the cages is the minimum, the situation that water flow is unsmooth, water quality is deteriorated, and lateolabrax japonicas are dead can be avoided, the lateolabrax japonicas can be always in a healthy living environment, and the survival rate of lateolabrax japonicas is increased.

The invention discloses Lateolabrax japonicus puffed feed and a preparation method thereof. The feed comprises the following ingredients by mass percent: 22-30% of steam fish meal, 20-28% of peeled soybean meal, 12-20% of soy protein concentrate, 15.5-26.5% of strong flour, 1-5% of corn protein powder, 4.5-8.5% of refined fish oil, 0.5-2% of soybean oil, 2-5% of monocalcium phosphate, 1-2% of premix of vitamin and mineral salt, 0.1-0.5% of choline chloride and 0.1-0.5% of free methionine. The feed has the advantages that the nutrition is more comprehensive, the collocation of various ingredients is more balanced, the feed conversion ratio is high, thereby improving the immunity and the survival rate of Lateolabrax japonicus and reducing fatty liver phenomenon caused by feeding in the cultivation process.

The invention discloses a long-term safe breeding method for lateolabrax japonicus juvenile fish. Initial lateolabrax japonicus juvenile fish bodies with the mass of 14.11+/-0.45 g in the early stageare selected, and the safe alkalinity of long-term breeding is 10 mmol/L. The method has the advantages that the setting of the safe alkalinity of the lateolabrax japonicus juvenile fish is firstly opened to the public according to the long-term breeding experimental result, the alkalinity is 10 mmol/L, early alkalinity adaptation can enhance the higher alkalinitytolerance of the lateolabrax japonicus juvenile fish, in actual production, data support is provided for the dividing of south and north groups of lateolabrax japonicas, and technical support is provided for salt and alkali water areapopularization and geographical population identification of early lateolabrax japonicas fry.

The invention relates to a lateolabrax japonicus red-skin disease treating feed supplement and a preparation method thereof. The lateolabrax japonicus red-skin disease treating feed supplement is prepared from, by weight part, 25-30 parts of kudzuvine root, 21-27 parts of portulaca oleracea, 12-18 parts of radix bupleuri, 11-17 parts of bunge corydalis herb, 9-15 parts of liquorice, 12-15 parts of potentilla discolor, 14-20 parts of lotus leaf, 5-7 parts of pagoda tree pod, 4-6 parts of bitter gourd, 3-4 parts of fructus forsythiae, 7-10 parts of hypericum japonicum, 9-14 parts of gallnut, 11-18 parts of dandelion, 7-10 parts of rabdosia lophanthide, 9-12 parts of herba cepbalanoplosis segeti, 8-10 parts of radix prismatomeris connatae, 11-15 parts of Chinese pulsatilla root, 7-10 parts of ash bark, 3-5 parts of wartwort, 1-2 parts of green tea, 2-5 parts of Chinese hawthorn leaf and 27-35 parts of water earthworm. The lateolabrax japonicus red-skin disease treating feed supplement can effectively treat lateolabrax japonicus red-skin disease.

The invention is applicable to the technical field of biological detection, and provides application and an application method of an atp1b1b gene in identification of sea-freshwater cultured lateolabrax japonicus. As an effective molecular marker for identifying sea freshwater cultured lateolabrax japonicus, the gill tissue expression level of the atp1b1b is closely related to ion transport; by detecting the expression level and the expression position of the atp1b1b gene in the gill tissue of the lateolabrax japonicus, the lateolabrax japonicus individuals cultured in sea and freshwater can be quickly and accurately distinguished.

The invention provides a lateolabrax japonicus aquaculture method. The method is characterized by comprising the following steps of (1) pond preparation, (2) preparation before stocking, (3) lateolabrax japonicus stocking, (4) feed feeding, (5) water quality management and (6) disease prevention management. Accordingly, in the aquaculture process, adopted synthetic feed is good in palatability, the food intake of the lateolabrax japonicus is increased, and the growth rate of the lateolabrax japonicus is increased; a disease-prevention solution sprayed every 20-30 days can effectively reduce the morbidity rate of the lateolabrax japonicus, thus the death rate of the lateolabrax japonicus is lowered, and the economic benefit of breeders is guaranteed.

The invention provides a fry breeding device and method of lateolabrax japonicus. The device comprises an oxygen supply motor, a turbine chamber, an air inlet grid, a turbine blade, a jet rod, an auger motor, an auger shaft, a sliding groove, a squeezing groove and a discharge port, wherein the turbine chamber is formed in the left side of a transverse rod, the upper side of the turbine chamber is provided with the oxygen supply motor, the front end face of the turbine chamber is provided with the air inlet grid, the inner portion of the turbine chamber is assembled with the turbine blade, and the lower side of the turbine chamber is connected with the jet rod. The fry breeding device and method of the lateolabrax japonicus solve the problem that an existing lateolabrax japonicus fry oxygen supply device is not even and effective enough in oxygen supply; the right side of the transverse rod is provided with the sliding groove, the upper side of the inner portion of the sliding groove is provided with the auger motor, the lower side of the auger motor is connected with the auger shaft, the lower side of the sliding groove is provided with the squeezing groove, and the left side of the squeezing groove is provided with the discharge port, so that the fry breeding device and method of the lateolabrax japonicus solve the problem that the digestion of lateolabrax japonicus fry is not facilitated due to the fact that original fry breeding feeding particles are larger. The fry breeding device and method of the lateolabrax japonicus has the advantages that the structure is reasonable, and the increase of the survival rate of the lateolabrax japonicus fry breeding is facilitated.

The invention discloses a lateolabrax japonicus interleukin IL-12p40 gene and the application thereof. The lateolabrax japonicus interleukin IL-12p40 gene has the nucleic acid sequence shown as SEQ IDNO. The invention further discloses encoded protein of the lateolabrax japonicus interleukin IL-12p40 gene. The encoded protein has the amino acid sequence shown as SEQ ID NO.2. The invention furtherdiscloses an expression vector comprising cDNA and the application of the encoded protein or recombinant protein of the lateolabrax japonicus interleukin IL-12p40 gene to preparation of medicines with antibacterial and fish immunity enhancing efficacies.

The invention discloses a method for judging lateolabrax japonicus aquatic water. The method specifically comprises the following steps: extracting total RNA (Ribonucleic Acid) from lateolabrax japonicus tissues to be judged, and synthesizing a first cDNA (complementary Deoxyribonucleic Acid) chain; amplifying a prolactin receptor via a PCR (Polymerase Chain Reaction); observing whether a prolactin receptor expression strap exists on the gel electrophoresis picture of a PCR amplification product or not in order to judge whether lateolabrax japonicus under detection is cultured in fresh water or sea water. The method disclosed by the invention is simple and accurate, and can be applied to supervision in the field of aquatic products.

The invention discloses a processing technology of lateolabrax japonicus meat floss. The processing technology comprises the following steps: (1) pretreating a raw material; (2) marinating in seasonings; (3) steaming for flavoring; (4) removing bones and drying; (5) preparing the meat floss and packaging: putting dried fillets into a stirrer, stirring into a flossy shape, and then sterilizing andpackaging. By the processing technology, the advantages of less bones and delicious meat of lateolabrax japonicus are combined; by the pertinent processing technology, the lateolabrax japonicus is processed into a fish meat floss product with a unique flavor; the meat floss product is good in mouth feel and loose in fish meat texture and is easily digested and absorbed by a human body, and is suitable for any group including infants and old people.