10 results about "Lateolabrax" patented technology

The invention discloses a method for separating, purifying and transplanting spermatogonial stem cells of lateolabrax japonicus, the spermatogonial stem cells of lateolabrax japonicus are separated and purified, marine fish larvae or freshwater fish larvae are used as receptor larvae, and then the spermatogonial stem cells of lateolabrax japonicus are transplanted to abdominal cavities of the receptor larvae. The spermatogonial stem cells obtained through separation are complete in number, structure and function and low in cell clustering rate. After the transplanted spermatogonial stem cells are subjected to fluorescence labeling, the migration and proliferation processes of donor germ cells can be observed in receptor fishes of marine fish fries or freshwater fish fries, so that the receptor fishes with the chimeric germline are obtained. According to the method, through xenotransplantation of the spermatogonial stem cells, a'belly borrowing reproduction 'breeding mode is provided for the marine fishes, a new way for gamete preservation and rapid propagation is provided for the marine fishes with the long reproductive cycle, and a new method and a new thought are provided for genetic improvement and germplasm resource preservation.

The invention discloses application of sea cucumber peptide in preparation of feed for relieving high-temperature heat stress of lateolabrax japonicus. The sea cucumber peptide added into the feed can improve the growth performance of the lateolabrax japonicus; the antioxidant capacity of serum of lateolabrax japonicus is improved; the non-specific immunocompetence of serum of lateolabrax japonicus is improved; the intestinal digestive enzyme activity of lateolabrax japonicus is improved; energy metabolism related indexes of lateolabrax japonicus are improved; the organelle stability of the lateolabrax japonicus liver cells is improved.

The invention discloses an identification primer group of lateolabrax japonicus dnd genes and application of the identification primer group in identification of lateolabrax japonicus germ cells, and belongs to the technical field of fish germ cell identification. The complete cDNA sequence of the dnd gene of the lateolabrax japonicus is provided, a specific primer is obtained by comparing the cDNA sequence, and the germ cells of the lateolabrax japonicus can be quickly and accurately distinguished by utilizing a PCR (Polymerase Chain Reaction) technology. The primer group provided by the invention has strong specificity on the dnd gene of lateolabrax japonicus, and can efficiently and accurately distinguish germ cells of lateolabrax japonicus.

The application belongs to the technical field of feed and discloses a kind of mixed feed for enhancing the resistance to Nocardia infection of Lateolabrax japonicus. The application discloses, for the first time, the use of citric acid and / or oregano oil in the preparation of products for preventing or treating diseases caused by Nocardia infection of Lateolabrax japonicus. Experiments show that the addition of citric acid and / or oregano oil to feed can effectively improve the non-specific immune capacity of Lateolabrax japonicus and further improve the survival rate of Lateolabrax japonicus after Nocardia infection. The amount of citric acid and / or oregano oil added is small, and the survival rate of Lateolabrax japonicus after Nocardia infection can be improved by feeding. The addition of citric acid and / or oregano oil does not adversely affect Lateolabrax japonicus, is easy to operate, and has high feasibility.

The invention discloses a lateolabrax japonicus testis cryopreservation solution which comprises the following components: an MEM culture medium, 35.2% of a buffer solution, 1.3-1.6 M of dimethyl sulfoxide, 0.1-0.3 M of trehalose and 10% of fetal calf serum, and the concentrations are all the concentrations in the MEM culture medium. The invention also discloses an ultralow-temperature cryopreservation method for the testis of the lateolabrax japonicus, which comprises the following steps: adding the testis of the lateolabrax japonicus into the cryopreservation liquid, freezing at-80 DEG C, and transferring to liquid nitrogen for cryopreservation. By adopting the testis cryopreservation liquid provided by the invention, the lateolabrax japonicus testis is subjected to ultralow-temperature cryopreservation, the prepared spermatogonium after recovery still survives, and the survival rate is more than 50%. Reliable donor guarantee is provided for germ cell transplantation, so that the application range of fish germ cell transplantation is remarkably widened.

The invention provides a deep learning-based multi-modal phenotype determination method for economic traits of lateolabrax japonicus, and relates to the technical field of high-throughput phenotype determination of economic traits of lateolabrax japonicus. According to the deep learning-based multi-modal phenotype determination method, multi-angle video streams are synchronously acquired by utilizing a sensor array, a temporary visual identifier is allocated to each tail lateolabrax japonicus, and then, a visual identifier is allocated to each tail lateolabrax japonicus; when a preset underwater measurement condition is met, a video clip in a time window is called, the divergence value of a multi-view motion vector field is calculated, a group of optimal key frames are selected from the clip in combination with the body principal axis direction and the lateral line contour definition, multi-view feature fusion and deformation compensation based on the divergence value are performed on the optimal key frame group, and the multi-view motion vector field is obtained. The method comprises the following steps: extracting static three-dimensional phenotypic characteristics, calculating dynamic behavior characteristics from a motion track, collecting the two types of characteristics according to identifiers to form underwater multi-modal phenotypic vectors, inputting the multi-modal phenotypic vector set of a group into a pre-trained economic character prediction model, and outputting individual predictive character indexes and group statistical results in batches.

The invention provides a controllable specific-pathogen-free lateolabrax japonicus seedling raising method, and belongs to the technical field of aquaculture. The method comprises the following steps: performing gradient salinization domestication on lateolabrax japonicus parents with negative viruses in a specific pathogen management and control list, performing intensified ripening cultivation, performing virus negative screening to obtain sexually mature lateolabrax japonicus parents, performing induced spawning under sterile conditions, and collecting mature ova and semen for insemination; the obtained fertilized eggs are disinfected and subjected to virus negative screening, and then are hatched in a water body containing bacillus subtilis and nitrifying bacteria; and culturing the obtained larva fish in a micro-ecological system containing chlorella, bacillus subtilis and nitrifying bacteria, carrying out virus negative screening, then carrying out gradient bait transfer culture and continuous virus negative screening, and then, carrying out seedling emergence. According to the method, the comprehensive survival rate in the whole process reaches 28.6%, more than 12100,000 seedlings emerge in a single batch, specific pathogens in the whole process are zero-positive, the SPF seedling standard is met, and the breeding survival rate after deep sea net cage putting is increased by 32% compared with common seedlings.

The invention discloses application of pomegranate polyphenol to preparation of feed for relieving high-temperature heat stress of lateolabrax japonicus. The pomegranate polyphenol added into the feed can improve the growth performance of the lateolabrax japonicus; the antioxidant capacity of serum of lateolabrax japonicus is improved; the non-specific immunocompetence of serum of lateolabrax japonicus is improved; energy metabolism related indexes of lateolabrax japonicus are improved; the intestinal digestive enzyme activity of lateolabrax japonicus is improved; related indexes of intestinal health of lateolabrax japonicus are improved; related indexes of intestinal tissue structures of lateolabrax japonicus are improved; the invention discloses application of a feed additive for improving the intestinal flora microbial structure of lateolabrax japonicus.

The invention discloses an application of lateolabrax japonicus vasa 3 'UTR (Untranslated Region) in marking primordial germ cells of sclerobone fish. EGFP-Lmvas3 'UTR mRNA is injected into 1-2 cell stage fertilized eggs of lateolabrax japonicus, zebra fish and oryzias latipe respectively, and results show that vas3' UTR of lateolabrax japonicus can realize visualization of a migration path of PGCs of lateolabrax japonicus from a body stage to after incubation, can be used for PGCs of two types of fishes, namely zebra fish and oryzias latipe, and shows that vas3 'UTR of lateolabrax japonicus has certain cross-species universality.

The invention discloses a large yellow croaker iridovirus double-antigen subunit vaccine and a preparation method thereof. By optimizing the sequences of the large yellow croaker iridovirus MCP protein and ATPase protein, the immunogenicity and stability of the large yellow croaker iridovirus MCP protein and ATPase protein can be remarkably improved, the expression quantity of the large yellow croaker iridovirus MCP protein and ATPase protein in baculovirus can be remarkably improved, and the two recombinant proteins are expressed by using a baculovirus expression system, so that large-scale production of the large yellow croaker iridovirus MCP protein and ATPase protein can be realized through large-scale serum- the production batch is stable, the protein expression quantity is high, and the production cost is low. According to the large yellow croaker iridovirus double-antigen subunit vaccine prepared by mixing the two recombinant proteins, the level of antibodies generated by immunizing large yellow croakers is high and is far superior to that of existing single-antigen vaccines and inactivated vaccines, the protection effect is strong, the protection effect of 78.1% or above can be obtained only through one-time immunization with a very small amount, and the large yellow croaker iridovirus double-antigen subunit vaccine is free of pathogenicity to the large yellow croakers and high in safety.