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34 results about "Anaerobic incubation" patented technology

Acid-resistant bifidobacterium breve BB8dpH and application thereof

The invention provides an acid-resistant bifidobacterium breve BB8dpH and an application thereof. The nucleotide sequence of the 16SrRNA gene part of the bifidobacterium breve BB8dpH is shown in SEQ ID NO.1, and is stored with the preservation number of CGMCCNo.8370. The bifidobacterium breve BB8dpH is a strain obtained by separating from the dejecta of healthy young people and further screening under the pH 3.2 acid condition. According to the fermentation and culturing process, anaerobic culture at 37 DEG C is carried out in a BL liquid substrate (containing 0.05% cysteine hydrochloride) for 24 hours. The bifidobacterium breve BB8dpH has remarkably better acid resistance than the common strains, and the acid resistance has genetic stability; the bifidobacterium breve BB8dpH has different percent contents of cell membrane fatty acids from the common strains, the average carbon chain length of the bifidobacterium breve BB8dpH is remarkably longer than that of the common strains, and the cell membrane fluidity of the bifidobacterium breve BB8dpH is remarkably lower than that of the common strains; and the bifidobacterium breve BB8dpH is used in the production field of daily fermented food, health-care food and medicines.
Owner:SHANGHAI JIAO TONG UNIV

Method for degrading organic pollutant through nanometer material photo-reduction and assisted by electricigens

The invention relates to a novel pollutant processing method for nanometer material photo-reduction degradation and assisted by electricigens. The method combines photo-reduction nanometer material and electricigens and remarkably improves reduction degradation capability of the electricigens on pollutant. The method belongs to the treatment field of water, waste water, sewage or sludge. The method includes adding a photo-reduction nanometer material into an electricigens growth medium, introducing electricigen ShewanellaoneidensisMR-1 to conduct anaerobic culture. In the culture process, the photo nanometer material obtains electron transfer from the electricigens and improves electron energy by illumination excitation, so that pollutant reduction and degradation capability of electrons is improved. The novel degrading method is suitable for reduction degradation treatment of various organic pollutants. By means of the method, a new treatment process and a new technical method can be provided for high-efficiency treatment of pollutant like industrial printing and dyeing waste water. The method has important actual application value and meets national requirements for sustainable development strategy.
Owner:JIANGSU UNIV

Biological incubator for experiment

The invention discloses a biological incubator for an experiment. Comprising a cabinet body, a culture room, a box door, a display, an operation button, a switch, a first storage room, a second storage room, a power supply, an integrated circuit board, a processor, a memory, a digital-to-analog converter, a temperature control system, a humidity control system, an oxygen concentration control system, a carbon dioxide concentration control system and a gas circulation system. The three culture rooms are arranged, various microorganisms and cells can be cultured at the same time, appropriate culture condition setting can be conveniently carried out on each microorganism or cell, the independent requirements of different microorganisms or cells during culture are met, for example, oxygen in the culture chambers can be eliminated during anaerobic culture; after culture conditions are set, different parameter requirements such as temperature, humidity, oxygen concentration and carbon dioxide concentration of the three incubators can be automatically regulated and controlled at the same time; and the three culture rooms are all independent spaces, so that the situation that microorganisms or cells are mutually polluted during culture is avoided.
Owner:YUNNAN UNIV OF TRADITIONAL CHINESE MEDICINE

Method and device for eliminating bad odor in pig farm

The invention relates to a method of elimating adour in a pig farm and a device, belonging to enginery. A pig toilet is arranged in a piggery, causing the pig to have the habit of relieving the bowels in the piggery, and causing the urine to be separated from swine waste in the toilet, separated urine flows into a sewage collecting channel through a floor, after through process of sewerage treatment program, applied for flushing the toilet circularly, while the swine waste is sucked to a high soil pail in a movable vacuum dung sucking cart, then fed to an anaerobic incubation groove of sewerage treatment programs through a vacuum pump for storage, and is added with microorganism bacterium, which is fully fermented as organic fertilizer. The invention has the advantages of saving manpower and water for cleaning the piggery, eliminating adour in the piggery, convenient usage, good effect and strong practicability.
Owner:廖 贯廷

Preparation method of porous nitrogen-doped graphene/carbon nanotube composite conductive agent

The invention discloses a preparation method of a porous nitrogen-doped graphene / carbon nanotube composite conductive agent. The preparation method comprises the steps of inoculating phanerochaete chrysosporium into a seed expansion culture solution for aerobic culture to obtain a seed liquid; adding the seed liquid and a graphene suspension liquid into a nutrition limitation culture solution, and dispersing the product subjected to constant-temperature shaking culture and processing in deionized water to obtain a nanopore graphene oxide suspension liquid; mixing the product and a carbon nanotube suspension liquid, performing ultrasonic processing, adding the mixed suspension liquid to an anaerobic denitrifying bacteria culture solution, and inoculating denitrifying bacteria seed liquid for anaerobic culture to obtain the porous nitrogen-doped graphene / carbon nanotube composite conductive agent. A two-step green preparation method is employed, a toxic reagent is not used, the preparation method has the advantages of moderate and controllable reaction process and low cost, and is easy to promote, and the prepared porous nitrogen-doped graphene / carbon nanotube composite conductive agent can be widely applied into the fields of a lithium ion battery and the like.
Owner:HEFEI GUOXUAN HIGH TECH POWER ENERGY

A process for producing tea product

The present invention relates to a process for producing a black tea product with enhanced sensorials. The people who prefer to consume black tea are unable to get a good amount of catechins, because, in black tea the amount of catechins is significantly less than that of green tea. Therefore there is a need for providing a tea product which has relatively high amount and catechins high amount of theaflavins and high amount of polyphenols made by a process without addition of any exogenous theaflavins and / or catechins and which also has the sensorials of black tea. It is an object of the present invention to provide a process for producing a tea product with a relatively high amount of catechins and theaflavins without the addition of any exogenous theaflavins and / or catechins. The present inventors have surprisingly found that tea products obtained by a process involving a step of anaerobic incubation at specific temperatures and for specific durations provide a tea product with improved sensorials and thereby satisfying one or more of the objects of the invention.
Owner:EKATERRA RES & DEV UK LTD

Paraquat quick detoxifying liquid and method

The invention discloses paraquat quick detoxifying liquid and a method. A preparation is a mixture of performing the light shading anaerobic cultivation for anthraquinone, Fe(III) humus reducing bacteria and a culture medium thereof, an electron donor and a pH buffering agent until enough anthraquinone is reduced as hydroquinone. The preparation is contacted and reacted with paraquat solution to quickly form a needle-shaped solid; and the needle-shaped solid is not dissolved in strong acid and strong alkali, is not dissolved in water and common organic solvent, is incapable of being absorbed by humans or animals, is more stable in normal-temperature and normal-pressure properties, and realizes quick paraquat detoxification. The preparation is simple in preparation method and low in cost.
Owner:ENVIRONMENT & PLANT PROTECTION INST CHINESE ACADEMY OF TROPICAL AGRI SCI

Chicken microecological preparation and preparation method thereof

ActiveCN104212745APrevention and treatment of intestinal diseasesGood prevention effectFungiBacteriaDrug biological activityFermentation
The invention relates to a chicken microecological preparation and a preparation method thereof and belongs to the technical field of preparation of microbial preparations. The chicken microecological preparation provided by the invention is prepared by diluting whole intestinal contents of a chicken with a culture medium and then filtering to obtain filtrate; further inoculating the filtrate into the culture medium, and culturing in an anaerobic culture system at the temperature of 40.5-41.5 DEG C for 35-37h to obtain a seed solution of a culture solution of intestinal bacteria; and finally inoculating the seed solution into a fermentation tank, filling nitrogen into the tank, keeping the pressure at 0.018-0.022MPa, keeping the pH at 6.8-7.0, and performing static culture at 40.5-41.5 DEG C for 46-50h. The microecological preparation is an all-weather, multi-bacterial species, union, coordination, overlapping and joint action biogenic compound preparation which has no toxicity, no side effects, no rejections, no pharmaceutical residues, no hormone residues and no production of drug resistance of bacteria. The content of effective living bacteria is not lower than 10 billion / ml; the effective period of biological activity of main probiotics is prolonged to 8 months at normal temperature.
Owner:山东省健牧生物药业有限公司

Method for manufacturing compost tea with lawn grass and application of compost tea

The invention discloses a method for manufacturing compost tea with lawn grass and application of the compost tea. The method includes steps of 1, collecting fresh plant materials-lawn grass in a current year; 2, adding various components into a closed container according to an EM (effective microorganism) strain-brown sugar-table salt-urea-sterile water proportion of 10:100:3:5:1000 w / w, carrying out anaerobic cultivation at the temperatures of 35 DEG C for 2 d to obtain EM activated bacteria solution; sufficiently drying and smashing the fresh lawn grass, then weighing 1-5 kg of fresh lawn grass, placing each type of materials into a heat-insulation tank, adding 1.5-7.5 kg of activated bacteria solution with EM bacteria into the heat-insulation tank, fermenting each batch of materials for 2 months, then placing thoroughly decomposed materials in plastic buckets with the volumes of 20-100 L, to be more specific, placing 0.5-2.5 kg of raw materials into each bucket, adding 18-45 L of water into each bucket, continuously filling the buckets with air for 3 d, then filtering the raw materials by the aid of filter screens and removing filter residues to obtain liquid which is the compost tea. The method and the application have the advantage that certain crop growth promotion effects can be realized by the compost tea.
Owner:NORTHWEST A & F UNIV

Operation table convenient for storing anaerobic culture medium

The invention provides an operation table convenient for storing an anaerobic culture medium, and relates to the field of biological experiment equipment. The operation table convenient for storing the anaerobic culture medium comprises an operating table body, the top end of the operating table body is slidably connected with an insertion plate, the inner top wall of the operating table body is fixedly connected with a storage chamber, the bottom end of the inner wall of the storage chamber is rotatably connected with a storage disc, and the center position of the storage disc is provided with an air suction machine. When the operation table is used, a power shaft drives a cam and stirring teeth to rotate, the stirring teeth drive a storage disc to rotate, the storage disc drives a placement box to rotate, meanwhile, the cam rotates to drive one end of a downward pressing rod to move downwards, the other end of the downward pressing rod drives a first swing rod to warp upwards, the first swing rod drives a first ejector rod to warp upwards, and the first ejector rod drives a connecting lantern ring to move upwards. The connecting lantern ring is matched with the fixed rod to drive the second ejector rod to upwarp, the second ejector rod drives the fixed lantern ring to move upwards, the fixed lantern ring drives the movable rod to move upwards, and the movable rod moves upwards to drive the placement box to move out of the operation table body. The operation table has the advantages of being convenient for storing the anaerobic culture medium.
Owner:陈疑

Preparation method and application of environment-friendly tilapia mossambica feed

PendingCN114403309AImprove full binding abilityQuality improvementFungiBacteriaAnti nutrientsNutrition
The invention belongs to the technical field of feed, and discloses a preparation method and application of environment-friendly tilapia feed, the preparation method comprises the following steps: inoculating probiotics to a slant culture medium for culture to obtain slant bacterial liquid, then inoculating the slant bacterial liquid to a liquid culture medium for anaerobic culture to obtain source bacterial liquid; inoculating the source bacterial liquid into a fermentation culture medium, and performing cultivation and symbiosis to obtain a strain infiltrating liquid; adding corn, bran, wheat flour, rapeseed dregs, distillers' grains, soybean meal and dried small shrimps into the strain infiltration liquid, mixing and stirring, and fermenting to obtain a fermented material; and granulating the fermented material, and spraying the coating liquid to obtain the tilapia mossambica feed. The vinasse, the rapeseed meal and the soybean meal are added into the raw materials and can be matched with fermentation of the zymophyte liquid to take out self fibers, crude protein nutrition in the raw materials is supplemented, meanwhile, addition of the raw materials such as bran and corn is reduced, and metabolic activity of microorganisms is fully utilized, so that the nutritional value of the raw materials is increased. The purpose of decomposing and converting anti-nutrient substances in the fermentation raw materials is achieved.
Owner:江门市旺海饲料实业有限公司

Method for adsorbing tetracycline in underground water by using green rust

The invention discloses a method for adsorbing tetracycline in underground water by using green rust. The method comprises the following steps: adding 0.5-8g / L green rust into a reaction bottle filledwith a solution with TC initial concentration of 1-80mg / L in an anaerobic incubator, tightly capping the bottle, placing the bottle on a rotary incubator to rotate away from light at a rotating speedof 30-70 r / min, performing timing sampling, and performing solid-liquid separation on obtained samples through a 0.45-micron filter membrane so as to achieve tetracycline adsorption. The method provided by the invention for adsorbing tetracycline in underground water by using green rust can effectively control the potential toxic substance TC in the underground water and ensure the safety of thedrinking water source of the underground water.
Owner:ZHEJIANG UNIV OF TECH

Method for removing uranium in enriched ryegrass degradation raffinate by microorganisms under anaerobic condition

The invention discloses a method for removing uranium in enriched ryegrass degradation raffinate of by microorganisms under an anaerobic condition. The method comprises the following steps: adding enriched ryegrass degradation raffinate, an electron donor, anthraquinone-2-sulfonate and an inorganic salt culture medium into a culture container, then inoculating microorganisms, introducing pure nitrogen into the culture container, putting the culture container into an anaerobic incubator, carrying out static culture, and then carrying out centrifuging; before the anaerobic incubator is used, using pure nitrogen for gas replacement; adding FeCl2 into the centrifuged waste liquid, oscillating the waste liquid, and then centrifuging the waste liquid; adding an alkaline substance into the centrifuged waste liquid, adjusting the pH value to 11, and oscillating the waste liquid; and removing the uranium in the enriched ryegrass degradation raffinate. According to the method, uranium in the enriched ryegrass raffinate is removed through microorganisms combined with a chemical method, uranium and liquid are separated, the waste liquid meets the discharge requirement, and the removal efficiency of U (VI) in the enriched ryegrass raffinate reaches 99.90% or above.
Owner:SOUTHWEAT UNIV OF SCI & TECH

Method for treating tail water of aquaculture pond

The invention belongs to the technical field of aquaculture, and discloses an aquaculture pond tail water treatment method, which comprises the following steps: carrying out primary filtration treatment on tail water, then carrying out precipitation treatment to precipitate sludge and excrement in the tail water, and then carrying out secondary filtration treatment to obtain a first water phase and a first solid phase; step 2, performing anaerobic culture domestication on the first solid phase to obtain a second solid phase; mixing the second solid phase with the first water phase, and sequentially carrying out denitrification and aeration treatment so as to remove ammonia nitrogen in the tail water and accelerate oxygenolysis of organic matters at the same time; and step 3, adding a treating agent and an adsorbent into the tail water subjected to aeration treatment, uniformly mixing, and reacting for 8-16 hours, so that the tail water is purified in the tail water, and the purified tail water is obtained. Physical treatment, chemical treatment, microbiological treatment and agricultural waste are combined, the aquaculture tail water treatment effect is good, the operation cost is low, and application and popularization are facilitated.
Owner:SHANXI AGRI UNIV

Culture method of low-temperature anaerobic sludge

PendingCN112723537ASolve the problem of substandard treatment in winterConditions affecting useSludge treatmentWater contaminantsActivated sludgeSewage
The invention discloses a culture method of low-temperature anaerobic sludge, which is used for obtaining low-temperature anaerobic sludge treatment domestic sewage and belongs to the technical field of sewage treatment. The method comprises the following steps: carrying out enrichment culture on activated sludge obtained in a chemical wastewater treatment process and sludge at the bottom of a septic tank, dehydrating the sludge subjected to enrichment culture, mixing the two kinds of enriched sludge to obtain mixed sludge, carrying out alternate anaerobic culture on the mixed sludge by using artificial sewage and rural domestic sewage, and collecting the mixed anaerobic sludge. The low-temperature anaerobic sludge which can treat domestic sewage at 8 DEG C to reach the discharge standard in GB5084-2005 Standard for Water Quality of Farmland Irrigation is obtained by adopting the anaerobic sludge as a raw material, stabilizing and domesticating at low temperature, can be used in a low-temperature domestic sewage treatment device, realizes sewage treatment in winter, greatly reduces the operation cost of sewage treatment in winter, and has important significance.
Owner:JILIN INST OF CHEM TECH

Intelligent parallel anaerobic culture fermentation system

The invention discloses an intelligent parallel anaerobic culture fermentation system. The intelligent parallel anaerobic culture fermentation system comprises a fermentation tank body (8), a nutrient solution dropwise adding device, an anaerobic environment control execution device and an air pressure monitoring module which are mounted on a visual operation table. Four-pipe-hole fermentation tank body design is adopted and is responsible for nutrient solution injection, nitrogen injection, air extraction and pressure monitoring correspondingly, so that nitrogen filling and gas extraction time is more accurate, an anaerobic environment with higher precision is manufactured, a signal is transmitted to the operation table by a pressure module and is visually displayed by an LCD (Liquid Crystal Display), and intelligent control of anaerobic fermentation is realized. According to the automatic control system designed by the invention, the production process is optimized, and an anaerobic microorganism fermentation process automatic control system with good functions is formed. The system aims to solve the problem that anaerobic conditions are difficult to make and control in the anaerobic bacterium fermentation process.
Owner:INNER MONGOLIA UNIV OF TECH

High-feeding-attraction and high-palatability organic trace element and preparation method thereof

PendingCN113768049ASolve the problem of low palatabilitySimple production processFungiAnimal feeding stuffBiotechnologyInorganic salts
The invention discloses a high-feeding-attraction and high-palatability organic trace element and a preparation method thereof. The preparation method comprises the following steps of: performing primary seed culture, secondary seed expanding culture and aerobic expanding culture on a flavor-producing yeast strain, and culturing for 3-4 hours in an anaerobic environment; continuously dripping a prepared high-concentration inorganic salt metal ion solution into fermentation liquor after anaerobic culture, and chelating for 2-3 hours under an anaerobic condition; heating, and fully autolyzing the flavor-producing yeast strain under a certain condition; and drying the autolyzed flavor-producing yeast strain solution by adopting a film drying method, and processing to prepare an organic trace element product. According to the method provided by the invention, the processes of centrifuging, concentrating, extracting, washing and the like are not needed in the production process, no organic solvent is discharged, the process is relatively simple, the process cost is low, and the method is suitable for large-scale industrial production. In addition, the prepared product solves the problem that the current organic trace element is low in palatability and has wide application prospects and market space.
Owner:上海源耀农牧科技有限公司

Anaerobic incubator capable of accurately controlling oxygen concentration and culture method based on anaerobic incubator

The invention discloses an anaerobic incubator capable of accurately controlling oxygen concentration and a culture method based on the anaerobic incubator, and belongs to the field of biological devices. The incubator comprises an incubator body, an air collection device and a gas separation device, the incubator body comprises a culture bin and an equipment bin which is separated from the culture bin in a sealed mode, the air collection device is arranged in the equipment bin, the end of the air collection device is communicated with the culture bin and used for collecting air in the culture bin, and one end of the gas separation device is communicated with the air collection device; the other end is communicated with the culture bin. Air in the culture bin is collected into the gas separation device through the air collection device, then oxygen in the air is separated out through the gas separation device, and oxygen-free gas in which the oxygen is separated out is discharged into the culture bin, so that it can be guaranteed that the oxygen in the gas in the culture bin is fully separated out; further, the oxygen concentration in the culture bin is accurately controlled.
Owner:南京烔创科技有限公司

A floating double-bed microporous membrane water restoration device

The invention discloses a floating type double-bed micro-porous membrane water body repairing device which comprises a floating plate, a photovoltaic assembly, an air conveying pump, a storage battery, a control center, an anaerobic jar, a liquid conveying pump, an aerobic bed, an anaerobic bed and a connecting rope. The aerobic bed and the anaerobic bed are sequentially suspended below the floating plate in series. Air is introduced into the aerobic bed so as to accelerate the reproduction of aerobic bacteria. The anaerobic bed conveys anaerobic bacteria through the anaerobic jar. Filled biological rope filler is beneficial for the adhesion reproduction of bacteria. Small holes are formed in shells of the aerobic bed and the anaerobic bed so that bacteria diffusion can be facilitated. Thestructure of the repairing device is beneficial for bacteria reproduction; a winding machine, an ultrasonic distance detector, a water body eutrophy detector, an electric propeller, an electric rudder, an anchor and a remote controller are additionally arranged, the water body eutrophy degree is judged, and the repairing device is automatically controlled by a control center or remotely controlled by manpower to move in a water body for repairing and purifying; aerobic bacteria and anaerobic bacteria are used for repairing the water body without being interfered with by each other, the growthand reproduction of the bacteria are rapid, and the water body repairing and purifying effect is good.
Owner:武汉新源水务环境工程有限公司

Method for comprehensively treating oyster shells based on anaerobic digestion

A method for comprehensively treating oyster shells based on anaerobic digestion comprises the following steps: step 1, drying oyster shells, and crushing and grinding the oyster shells to prepare oyster shell powder; step 2, uniformly mixing the oyster shell powder and the livestock and poultry manure, adding the mixture into an anaerobic digestion reactor, inoculating anaerobic fermentation organisms, and carrying out anaerobic culture at the anaerobic fermentation temperature of 35-38 DEG C for 20-30 days; 3, after gas production is finished, taking out the mixture of the oyster shell powder and the biogas residues from the fermentation reactor, and performing dehydration treatment to reduce the water content; 4, the dehydrated mixture of the oyster shell powder and the biogas residues is naturally air-dried and crushed, and the organic fertilizer is obtained.The oyster shell and biogas residue mixed organic fertilizer is prepared by combining the oyster shells with the anaerobic digestion process, so that resource utilization of the two kinds of waste including the oyster shells and the livestock and poultry manure is effectively achieved; meanwhile, by virtue of the synergistic effect between the two components, the organic fertilizer which can be effectively utilized is formed.
Owner:QUANZHOU INST FOR ENVIRONMENTAL PROTECTION IND NANJING UNIV

Preparation method of composite microbial agent for harmless treatment of fecal sewage

The invention discloses a preparation method of a composite microbial agent for harmless treatment of fecal sewage. The preparation method includes the following steps that three nutrient solutions are prepared, each nutrient solution is 50 liters, and the three nutrient solutions are marked separately and sterilized for use; a microbial bacterium A is inoculated into one nutrient solution, and light-free aerobic culture is conducted for 48 hours; a microbial bacterium B is inoculated into another nutrient solution, and light-free aerobic culture is conducted for 48 hours; a microbial bacterium C is inoculated into the remaining nutrient solution, the remaining nutrient solution is placed at the temperature of 28-30 DEG C, and light-free anaerobic culture is conducted for 48 hours; after 48 hours, the prepared bacterial solutions are mixed in a closed container, the closed container is placed at the temperature of 28-30 DEG C, light-free culture is conducted for 7-10 days, and gas discharge is conducted every 4-5 hours; when the pH is smaller or equal to 4.0, a finished product is prepared. The provided composite microbial agent has a simple preparation process, wide application range, stable performance, obvious effect, no toxic or side effects, and high practicability.
Owner:陈玉亭

Method for degrading organic pollutant through nanometer material photo-reduction and assisted by electricigens

The invention relates to a novel pollutant processing method for nanometer material photo-reduction degradation and assisted by electricigens. The method combines photo-reduction nanometer material and electricigens and remarkably improves reduction degradation capability of the electricigens on pollutant. The method belongs to the treatment field of water, waste water, sewage or sludge. The method includes adding a photo-reduction nanometer material into an electricigens growth medium, introducing electricigen ShewanellaoneidensisMR-1 to conduct anaerobic culture. In the culture process, the photo nanometer material obtains electron transfer from the electricigens and improves electron energy by illumination excitation, so that pollutant reduction and degradation capability of electrons is improved. The novel degrading method is suitable for reduction degradation treatment of various organic pollutants. By means of the method, a new treatment process and a new technical method can be provided for high-efficiency treatment of pollutant like industrial printing and dyeing waste water. The method has important actual application value and meets national requirements for sustainable development strategy.
Owner:JIANGSU UNIV
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