300 results about "Intestinal bacteria" patented technology

The present invention relates to nanosilver-containing antibacterial and antifungal granules ("NAGs"). The NAGs have longlasting inhibitory effect on a broad-spectrum of bacteria and fungi, which include, but are not limited to, Escherichia coli, Methicillin resistant Staphylococcus aureus, Chlamydia trachomatis, Providencia stuartii, Vibrio vulnificus, Pneumobacillus, Nitrate-negative bacillus, Staphylococcus aureus, Candida albicans, Bacillus cloacae, Bacillus allantoides, Morgan's bacillus (Salmonella morgani), Pseudomonas maltophila, Pseudomonas aeruginosa, Neisseria gonorrhoeae, Bacillus subtilis, Bacillus foecalis alkaligenes, Streptococcus hemolyticus B, Citrobacter, and Salmonella paratyphi C. The NAGs contain ground stalk marrow of the plant Juncus effusus L. which has been dispersed with nanosilver particles. The nanosilver particles are about 1-100 nm in diameter. Each of the nanosilver particles contain a metallic silver core which is surrounded by silver oxide. The present invention also provides a process for making the NAGs. The NAGs can be used in a variety of healthcare and industrial products. Examples of the healthcare products include, but are not limited to, ointments or lotions to treat skin trauma, soaking solutions or cleansing solutions for dental or women hygiene, medications for treating gastrointestinal bacteria infections, sexual related diseases, and eye diseases. Examples of industrial products include, but are not limited to, food preservatives, water disinfectants, paper disinfectants, construction filling materials (to prevent mold formation).

The present invention discloses a fruit and vegetable probiotic tablet and a preparation method thereof. The fruit and vegetable probiotic tablet uses probiotic powders such as lactobacillus plantarum powder as the main raw material, and the preparation method scientifically mixes modified dietary fibers, fruit and vegetable powder, oligosaccharides, plant extracts, protein powder, tea leaf extracts and traditional Chinese medicine extracts and etc., thus improves the content of soluble celluloses which are of real significance for probiotic flora, enhances the physiological activity of celluloses, thereby increases the species of intestinal probiotic flora as well as significantly enhances the colonization ability and time of endogenous and exogenous probiotics in the human intestinal tracts, effectively inhibits the growth and reproduction of harmful intestinal bacteria, especially gram-negative bacteria, and fully regulates the composition of the intestinal probiotic flora. The prepared fruit and vegetable probiotic tablet has a high biological activity, a long human intestinal colonization time, and a significant weight loss effect, and is suitable for a wide range of people.

Methods using in vitro processes are disclosed for inducing or enhancing expression of enteric bacterial antigens or virulence factors. The methods, therefore, produce antigenically enhanced enteric bacteria. Also methods for using the antigenically enhanced bacteria are also disclosed, as well as vaccines containing the enteric bacteria. Specifically a whole enteric bacterium or components thereof are provided by Helicobacter species. Also there are other enteric bacteria which are useful for the disclosed invention; such as Campylobacter jejuni.

The invention relates to active lactic acid bacteria capsules with a treatment and prevention effect on vaginitis. The lactic acid bacteria capsules capable of simultaneously adjusting dysbacteriosis of intestinal tracts and vagina and emphasizing treatment and prevention of the vaginitis are developed for achieving comprehensive effects. According to the technical scheme, excipient and lactic acid bacteria mixed bacteria powder are wrapped in the active lactic acid bacteria capsules with the treatment and prevention effect on vaginitis, and the lactic acid bacteria mixed bacteria powder comprises lactobacillus crispatus powder and other lactic acid bacteria subspecies powder with an effect of adjusting the intestinal flora. The active lactic acid bacteria capsules can be used for treating the vaginitis by adopting oral administration and intra-vaginal use; the oral lactobacillus can effectively improve the immunity of mucosa by stimulating the immune response of the intestinal mucosa, adjust the overall function state of a human body and well treat the vaginitis; and a large quantity of lactobacillus proliferated in the intestinal tract can inhibit growth of intestinal bacteria and reduce the risk of intestinal bacteria infection in vagina.

The present invention provides a bacterium belonging to the genus Bifidobacterium, which can exhibit a high viability in the storage under agitation and can produce a fermented food containing a large number of viable bacterial count. The present invention further provides a fermented food, which contains the bacterium belonging to the genus Bifidobacterium and exhibits various physiological effects such as inhibitory action against harmful intestinal bacteria and intestinal function controlling effects.

The present invention further provides a bacterium belonging to the genus Bifidobacterium having a rate of viability of 30% or more, when the bacterium is stored in the aerobic condition under agitation at 10° C. for 14 days after culturing in a medium containing milk as a main ingredient until obtaining a viable count of 1×10⁸ cfu/ml or more, and fermented food using the same.

The invention relates to an emission reduction dry-type culture method for producing nonreactive pork. The method uses pig feeds, which contain feedstuff additive premix, and active water agent to feed the pigs. An organic adhesive layer is laid inside a pig shed. The active water agent contains natural plant extract and bacillus subtilis, and is drunk by the pigs through a leak protection and filtering water device arranged inside the pig shed. The feedstuff additive premix contains NSP enzyme, micro-ecological agent, plant extract and acidifier. The method can prevent the pigs from having intestinal bacteria and viral diseases and respiratory diseases, and reduce the discharge of fecaluria and living water, harmful gas produced by pig fecaluria and stench, and through which prevents the breeding of flies. At the same time of producing nonreactive port, the method can reduce the pollution caused by excreta.

Heat-stabilized defatted rice bran (HDRB) is fermented with yeast to afford a food product having superior prebiotic for probiotic properties. Fermentation of HDRB with yeast yields a prebiotic composition that can promote the growth and/or activity of beneficial intestinal bacteria (probiotic) when consumed by an animal or human. The prebiotic and/or probiotic compositions can afford substances having desirable health effects when consumed. The yeast fermented, and the probiotic fermented extracts and residues will have several nutraceutical compounds including protein, phenolics, phytic acid, arabinose, bioactive isoflavones, dietary fibers and several others that will have health benefits. Inclusion of legumes during fermentation can synergistically enhance the bioactivities of HDRB. HDRB can be a source of growth medium for yeast/mold/microorganisms.

The invention discloses Chinese herbal medicine compound feed for beef cattle. The Chinese herbal medicine compound feed is prepared from the following materials in parts by weight: 45-50 parts of cornmeal, 15-18 parts of bean cakes or cottonseed cakes, 10-12 parts of wheat bran, 1.5-2 parts of calcium hydrogen phosphate, 1.2-1.5 parts of salt, 0.5-0.8 part of cactus powder, 2-3.2 parts of purslane, 1.5-1.6 parts of radix puerariae, 1.2-1.3 parts of tangerine peel, 3.5-4.3 parts of hawthorns and 1-4 parts of white atractylodes rhizome. The materials used for the feed are wide in source and low in price, nutritional ingredients contained in the materials fully meet the growth needs of the beef cattle, due to the addition of various Chinese herbal medicines, the gastrointestinal function of the beef cattle can be effectively improved, the digestion and the absorption are facilitated, the growth of the beef cattle is promoted, the resistance of the beef cattle to various intestinal bacteria is effectively improved, the immunity of the organism of the beef cattle is improved, and the demand for hormone drugs of the beef cattle is basically eliminated, so that the meat quality of the finally obtained beef cattle is relatively rich in nutrition.

The invention discloses a method for real-time quantitative polymerase chain reaction (PCR) detection of bifidobacteria and Escherichia coli by using Taqman probes. The method comprises the following steps of: A) extracting bacterial genome DNA of the bifidobacteria and the Escherichia coli in a sample to be detected; B) synthesizing primers and the Taqman probes according to related sequences of the bifidobacteria and the Escherichia coli; and C) ensuring that the primers and the probes obtained in the step B) and other PCR reaction reagents form a reaction system for real-time quantitative PCR by the Taqman probes. The Taqman probes are labeled by double colors, by a double absolute quantitative PCR method, two kinds of intestinal bacteria in a sample can be accurately quantified at one time, the detection cost and detection time can be saved, and errors caused by repeated detection are reduced.

The present invention relates to biotransformation process to synthesize intestinal diol as one kind of lignanoid plant estrogen. The biotransformation process synthesizes intestinal diol with human intestinal bacteria in 4, 4'-dihydroxy intestinal diol as substrate and under anaerobic condition. The process can produce intestinal diol in great amount and obtain pure (-)-intestinal diol. Intestinal diol has the bidirectional estrogen-like and estrogen-resisting regulation effect, and may be used in preventing and treating climacteric syndrome, osteoporosis, cardiac vascular diseases, cancer, etc.

The invention relates to enterococcus faecium Anp01 belonging to enterococcus. The enterococcus faecium Anp01 is preserved in China Center for Type Culture Collection (CCTCC) on January 19th, 2009, and the preservation NO is M209019. The enterococcus faecium Anp01 is used as a feed additive for improving the organism immunity. The enterococcus faecium Anp01 (CCTCC M209019) belongs to normal intestinal bacteria having no toxicity and intestinal pathogenicity to human beings and animals. The feed additive prepared by using the enterococcus faecium Anp01 can not generate any toxin even under abnormal conditions, can not generate potential hazards when the immunity function of host animals is low, and is a pure natural feed additive without any external drug resistance.

A feed composition for ruminants comprising a microbe having nitrite reductase activity, wherein said microbe has been modified so that intracellular nitrite reductase activity is enhanced is disclosed. A feed composition for ruminants comprising a microbe having nitrite reductase activity, wherein said microbe is selected from the group consisting of intestinal bacteria, coryneform bacteria, Bacillus subtilis, bacteria of the genus Methylophilus, Actinomyces, ruminal bacteria, and a combination thereof is disclosed. A method of improving the growth of a ruminant by administering the feed composition as described above to said ruminant and a method of preventing nitrite intoxication by administering the feed composition as described above to a ruminant are disclosed.

The invention belongs to the technical field of biological food and health care products and particularly relates to a health care product capable of promoting proliferation of intestinal bifidobacteria and an application. The health care product capable of promoting proliferation of the intestinal bifidobacteria comprises following components: 30%-60% of oligosaccharide, 0.4%-7.5% of a sweetener, 1.5%-2.5% of an acidulant, 10%-30% of plant polyphenol and the balance of filler. The health care product contains the oligosaccharide and the plant polyphenol, and after the health care product is taken by a human body, the plant polyphenol has an antioxidation function and can react with a large amount of hydrogen peroxide produced by lactic acid bacteria, so that bifidobacterium growth is protected directly; further, the plant polyphenol can inhibit a large quantity of harmful intestinal bacteria and has the function of regulating intestinal flora; besides, the intestinal pH value is reduced through short-chain fatty acid produced by bifidobacterium fermenting oligosaccharide, not only is bifidobacterium proliferation obtained, but also harmful bacteria are inhibited. The health care product can be used for preparing lozenges or capsules.

The invention discloses lactobacillus rhamnosus CCFM1060, a fermented food and a preparation method of a bacterial agent; increase of the abundance of Proteus induced by high lipid and high cholesterol diet is significantly reduced, and the occurrence of diseases such as urinary tract blockage, intestinal bacterial migration and enteritis is reduced; the lipid metabolism disorder of NAFLD patientsis significantly improved; insulin resistance of NAFLD mice is significantly improved; increase of glutamic-pyruvic transaminase and glutamic-oxalacetic transaminase in serum can be re-adjusted; theconcentration of low-density lipoprotein cholesterol is significantly reduced, and the risk of cardiovascular disease is reduced; the level of SOD in liver of NAFLD patients can be significantly improved; the inflammation of liver can be significantly improved; the damage of liver tissues of the NAFLD patients can be significantly improved; the expression of an Nrf2 gene in fatty liver cells can be significantly improved; the ability to absorb perfluorooctanoic acid in vitro is strong.

The invention discloses lemon jam used as biscuit stuffing and a preparation method thereof. The lemon jam comprises the following raw materials in percentage by weight: 60-70 percent of low-DE (Digestible Energy) glucose syrup, 10-20 percent of high fructose corn syrup, 10-20 percent of fructo-oligose, 1-5 percent of white granulated sugar, 1-5 percent of lemon peel powder, 0.5-1.5 percent of food gum and 1-3 percent of water. The lemon jam has the beneficial effects that beneficial ingredients of lemons are reserved in the jam, and fructo-oligose beneficial to intestinal bacteria is added; by virtue of the low-DE glucose syrup and the high fructose corn syrup, the consumption of white granulated sugar in the lemon jam is greatly reduced; in the lemon jam prepared by the preparation method, the water content is between 5 percent and 10 percent and the water activity is between 0.60 and 0.65, as biscuit stuffing, the lemon jam can be prepared into jam sandwiched biscuits with excellent flavor and taste, and within a shelf life of one year, the water of the biscuits is not remarkably increased.

Piglet early-stage care feed is disclosed. Raw materials include corn, puffed corn, soybean meal, fermented soybean meal, bran, puffed soybean, soybean oil, sugar, glucose, fish meal, amino acids, organic trace elements, inorganic trace elements, choline chloride, betaine, sodium chloride, calcium carbonate, calcium hydrophosphate, composite vitamins, composite enzymes, phytase resistant to high temperature, quinocetone, calcium oxytetracycline, 4-6 parts of an acidifying agent and kitasamycin. The acidifying agent is used in the feed to adjust acidity-alkalinity balance in intestinal tracts. Antibiotics are combined to resist intestinal bacteria. The feed can also improve the digestion rate and piglet immunity. An amino acid composition is adopted to improve immunologic balance of piglets. An organic trace element composition and zinc oxide are used to improve body immunity and intestinal health of the piglets. A composition of the white sugar, the glucose, monosodium glutamate, and the like is adopted to improve immunity and palatability. Beneficial effects of the method are that palatability is improved, the feed intake is obviously increased, intestinal tract stress is reduced, the diarrhea rate is reduced by about 20%, and the survival rate is increased by about 30%.

The invention discloses a compound fruit and vegetable enzyme and a preparation method thereof, wherein the preparation method comprises the specific steps: (1) mixing and crushing fruits and vegetables, wherein the fruits and vegetables are composed of the following raw materials: 10-30 parts of carrot, 10-30 parts of burdock, 10-30 parts of Chinese yam, 10-30 parts of raw ginger, 10-30 parts of cucumber, 10-30 parts of broccoli, 10-30 parts of aloe, 10-30 parts of spinach, 10-30 parts of pumpkin, 10-30 parts of apple, 10-30 parts of raw pear, 10-30 parts of grapefruit, 10-30 parts of pineapple, 10-30 parts of red jujube, 10-30 parts of hawthorn, 10-30 parts of papaya, 10-30 parts of orange, 10-30 parts of peach, 10-30 parts of lemon, 10-30 parts of plum, 10-30 parts of grape, 10-30 parts of mulberry, 10-30 parts of blueberry, 10-30 parts of strawberry, and 10-30 parts of durian; (2) adding an enzyme live keeping agent; (3) adding a bacterial fluid, sealing, and fermenting; and (4) filtering. The compound fruit and vegetable enzyme has comprehensive various extracellular enzymes, has strong vitality, can make in-vivo blood become slightly alkaline, eliminates waste in a body, maintains the balance of intra-intestinal bacteria, strengthens cells, promotes digestion, strengthens resistance, and makes a body keep balanced in various aspects.

The invention relates to the field of feed additives, in particular to a tea tree oil microemulsion animal nutrient and a preparation method thereof. The tea tree oil microemulsion animal nutrient of the present invention is natural, safe, green, pollution-free, drug-resistant, and drug-free It can significantly improve the quality of livestock and poultry meat products. Tea tree oil microemulsion animal nutrition has comprehensive functions, such as inhibiting harmful intestinal bacteria, enhancing immunity, promoting growth, improving production performance, improving feed conversion rate and anti-stress. It can replace antibiotics for livestock and poultry; at the same time, tea tree oil microemulsion animal nutrition can significantly inhibit the harmful intestinal bacteria such as Escherichia coli and Salmonella and the damage of mycotoxins in feed. The tea tree oil microemulsion animal nutrition agent screens out suitable emulsifiers and co-emulsifiers, which can solve the problems of tea tree oil being volatile and poorly stable active ingredients, and is of great significance for developing tea tree oil into a natural feed additive.

The invention relates to a disinfectant and a preparation method thereof. The formula of the disinfectant is: p-chloro-m-xylenol, coconut oleic acid, potassium hydroxide, isopropanol, pine oil and deionized water, and its weight percentage is, 5.0 -7.0: 6.5-9.5: 3.7-5.4: 15.0-25.0: 3.0-6.0: margin. The preparation includes: (1) adding deionized water and potassium hydroxide, stirring, heating up to 90°C-100°C, and then adding coconut oleic acid to make potassium coconut oleate soap; (2) mixing p-chloro-m-xylenol with Mix isopropanol, heat up to dissolve, then add deionized water and the above-mentioned potassium coconut oleate soap, stir; cool to 30°C-35°C, add pine oil, stir, add deionized water and continue stirring, and the product is ready. The disinfectant solution of the invention can effectively kill 99.999% of intestinal pathogenic bacteria, pyogenic cocci and pathogenic yeasts; the preparation method is simple, the cost is low, and it is suitable for industrial production.

The invention belongs to the technical field of the application of feed additives, and particularly relates to a novel application of a nano compound as a feed additive. The invention provides a novel application of the trehalose-montmorillonite nano compound as the feed additive, and preferably 1 to 2g/kg of the nano compound is added in the feed. The trehalose-montmorillonite nano compound has a positive effect on killing harmful intestinal bacteria, protecting intestinal mucosa and promoting the growth of animals and can substitute the conventional antibiotic-aureomycin of the feed additive. The trehalose-montmorillonite nano compound has the characteristics of safety and environmental friendliness. Through the application of the trehalose-montmorillonite nano compound, the defect that the antibiotics are widely applied in the breeding industry can be effectively overcome, and a safe animal product conforming to the internal standard can be produced.

The invention discloses a quantitative detection method of resistance genes of intestinal bacterial antibiotics; the method comprises the steps of S1, extracting total DNA of intestinal bacteria; S2, using the total DNA of the intestinal bacteria as a template, amplifying antibiotic resistance genes and 16SrRNA gene via PCR (polymerase chain reaction), and linking the amplification products to cloning vectors, and converting host bacteria to obtain positive clones; propagating the positive clones, and extracting plasmids, measuring copy density of the plasmids, diluting at 100 gradient to obtain a standard template, and establishing a standard curve for the copy number and Ct value through fluorescent quantitative PCR; S3, subjecting the positive plasmids and samples to fluorescent quantitative PCR amplification, and calculating the copy number of antibiotic resistance genes carried in the samples to obtain relative content of the antibiotic resistance genes. The method is good in safety and environmental friendliness and low in price, has simple steps, is low in time consumption, has stable and reliable results, has good repeatability, high sensitivity and high practicality, is simple and fast, and has a promising application prospect.

The invention discloses the application of intestinal bacteria in feces in the screening, diagnosis or auxiliary diagnosis of cholangiocarcinoma. The invention obtains the characteristics of bacterialspecies and abundance by extracting DNA of bacteria in feces and sequencing, and carries out diagnosis of cholangiocarcinoma on the basis of the bacterial abundance characteristics. Compared with thecurrent method for screening cholangiocarcinoma which has been used in clinic or patent application, the present invention is completely non-invasive and can more accurately predict and diagnose cholangiocarcinoma.

The invention discloses maize germ instant powder containing xylooligosaccharide. The maize germ instant powder is prepared by the following method: uniformly mixing 60-80% of maize germ meal with 2-4% of xylooligosaccharide, 6-12% of xylitol and 12-25% of skim milk powder, and sterilizing to obtain the maize germ instant powder containing xylooligosaccharide. According to the maize germ instant powder containing xylooligosaccharide, which is disclosed by the invention, the maize germ meal is compounded with the xylooligosaccharide to greatly increase the absorption rate of protein in the maize germ and remedy the defect of low absorption rate of plant protein, and meanwhile, the xylooligosaccharide also has the functions of promoting gastrointestinal peristalsis, proliferating beneficial intestinal bacteria and relaxing bowels. The maize germ instant powder is hydrolyzed by papain to increase the content of soluble protein and enable the xylooligosaccharide to well play the function of prompting protein absorption; the maize germ instant powder is hydrolyzed by alpha-amylase and cellulose to improve the absorption of soluble sugar, improve the solubility of the corn germ meal, reduce the agglomeration rate of the corn germ meal and perfect the cooking taste of the maize germ meal.

The invention provides a preparation method of potato starch processing juice lactic acid beverage. According to the method, potato starch processing juice is subjected to enzymolysis, and then odor-free potato starch processing juice lactic acid beverage with mild sour and sweet taste and good stability and without whey separation is obtained by taking Lactobacillus plantarum, Lactobacillus reuteri and Streptococcus thermophilus as fermentation strains through bio-fermentation engineering. The yogurt beverage product not only contains lactic acid bacteria of the existing commercially available similar products available in the market and has the beneficial function of adjusting the bacteria flora of intestinal tract and promoting absorption in nutrient of lactic acid bacteria, but also contains functional active substances, capable of resisting against oxidation, resisting against cancer and controlling blood sugar, in potatoes, thus being suitable for being consumed and drank by all kinds of people; and the preparation method is simple in process, suitable for scale application and good in market prospect.

A system whereby a substance which is orally taken and to be delivered into the lower digestive tract is selectively delivered into the lower digestive tract. More particularly, a system which makes it possible to surely and quickly deliver the aimed substance to the lower digestive tract without being affected by pH change in the digestive tract due to change in bacterial flora. Compositions disintegrating in the lower digestive tract characterized by containing a compound <A>, which has a molecular weight of 1000 or less and has a disulfide bond, and a polymer <B>, which has a molecular weight exceeding 1000 and is digested by enteric bacteria and/or undergoes softening, swelling or dissolution due to a decrease in pH; molded products with the use of these compositions; and preparations with the use of these molded products.

The invention provides a method of producing enterodiol and/or enterolactone. The method of producing enterodiol and/or enterolactone comprises the steps: using at least one of flaxseed meal, seaweed, sesame meal, rapeseed meal, rye bran, wheat bran, barley bran, corn bran, oat bran and burdock meal as a substrate, carrying out anaerobic culture of the substrate by using intestinal bacteria, and obtaining the enterodiol and/or the enterolactone. The method uses plant resources with low price and intestinal flora of mammals to directly convert the enterodiol and the enterolactone by a biotechnology, and culture fluid enables the contents of the enterodiol and the enterolactone in an extract to be larger than 50 percent (70.15 percent to 91.12 percent) through a simple purification process. The invention has the advantages of simple method, low production cost, no environment pollution, convenient industrial production, important scientific value and high social and economic benefits.