76 results about "Amino acid metabolism" patented technology

Compositions and methods are provided for the enhanced in vitro synthesis of polypeptides. In order to improve the performance of in vitro protein synthesis reactions, metabolic inhibitors, or manipulation of a source organism, is used to diminish or avoid the action of enzymes responsible for undesirable amino acids production or depletion. A homeostatic system may be used for production of ATP, where the required high energy phosphate bonds are generated in situ, e.g. through coupling with an oxidation reaction. The homeostatic energy source will typically lack high energy phosphate bonds itself, and will therefore utilize free phosphate in the reaction mix during generation of ATP. The homeostatic energy source is provided in combination with an enzyme that catalyzes the creation of high energy phosphate bonds and with an enzyme that can use that high energy phosphate bond to regenerate ATP.

Compositions and methods are provided for the enhanced in vitro synthesis of protein molecules, by optimizing the metabolism of amino acids present in the reaction mix, preferably all amino acids in the reaction mixture. By performing synthesis with extracts from genetically modified microbial strains that are deficient in multiple amino acid metabolizing enzymes reduces the enzymatic activities responsible for catalyzing these reactions and improves the overall yield of synthesis.

The present invention discloses filter paper sheet gas chromatography-mass spectrum analysis process of organic acid / amino acid metabolic product in urine. The process includes elution of urine collected with filter paper, oximation reaction, extraction, methyl silane derivation, and united detection with chromatographic instrument and mass spectrograph. The process of the present invention is superior in that the filter paper collection of urine favors remote analysis of genetic metabolic diseases; the oximation reaction processing favors alpha-ketonic acid detection for diagnosis of branched chain ketonic acid metabolic disorder; the extraction has high recovery rate of organic acid / amino acid metabolic product, high repeatability, less chromatographic impurity peaks and no pollution to chromatographic capillary column and mass spectrographic ion source; and the united detection is suitable for screening analysis of great amount of samples.

The invention discloses a genetically engineered bacterium for producing L-arginine and a construction method and application thereof. According to the invention, a gene for encoding carbamyl phosphate synthetase and a gene for encoding L-arginine biosynthetic pathway enzyme are integrated in escherichia coli; a synthetic route of arginine in escherichia coli and metabolic flux related to argininein a whole amino acid metabolic network are analyzed and reconstructed to obtain genetically engineered bacteria which are clear in genetic background, do not carry plasmids, are not mutated and canstably and efficiently produce L-arginine; and the genetically engineered bacteria have good L-arginine production capacity.

The invention discloses a screening method of grape wine yeast with low-yield ethyl carbamate and application of grape wine yeast in the production of grape wine. The screening method comprises the following steps of: screening out a bacterial strain with the low-yield ethyl carbamate from active dried yeast and mutant strains by adopting a structural analogue of arginine, i.e., canavanine resistance flat plate, wherein the activity dried yeast can be separated from nature or can be purchased in market, and the mutant strains are obtained through the mutagenesis of chemical mutagen. The method is designed by a yeast amino acid metabolism theory and is simple and convenient; and a target bacterial strain can be obtained through rapid separation. According to the screening method, the obtained resistance bacterial strain B1 can be numbered as F15-1-15 bacterial strain (CGMCC (China General Microbiological Culture Collection Center) NO. 4727) and is applied to the brewing of the grape wine in a small-sized fermented container; and on the basis of excellent brewing performance of the grape wine, the bacterial strain has the advantages of being capable of effectively and obviously reducing the content of the ethyl carbamate in the production of the grape wine and having the potential of industrial production and application.

A position developer for tumor is prepared by the two-step synthesis which can easily realize autosynthesis. It is specific amino acid metabolism PET developer for distinguishing tumor from inflammation.

This invention relates to plants and plant cells which express heterologous D-amino acid metabolizing enzymes and may therefore employ D-amino acids as a source of nitrogen. Methods and means are provided for selectively modulating the growth and stress tolerance of such plants using D-amino acids. The methods can be used either for detoxification of phytotoxic D-amino acids such as D-alanine and D-serine, thereby allowing for selection of plants comprising said D-amino acid metabolizing enzymes, or for enhancing toxicity of lesser phytotoxic D-amino acids such as D-isoleucine, thereby allowing for selection of plants not comprising said D-amino acid metabolizing enzymes.

The invention relates to an anti-fatigue Maca extract and a functional drink including the same. The anti-fatigue Maca extract is prepared from the following raw materials by mass: 0.8 to 1.2 of Maca powder, 0.1 to 0.3 of rhodiola root powder, 0.2 to 0.5 of yacon powder, 0.2 to 0.5 of crab apple powder and 0.08 to 0.12 of mint leaf powder. According to the invention, the Maca, rhodiola root and yacon are blended, so comprehensive anti-fatigue effect is exerted; the crab apple and mint leaf enable the beverage to has sour-sweet, cool and refreshing taste; through microwave treatment of Maca powder, the irritant and pungent taste of Maca beverages can be eliminated; and anti-fatigue accessory factors are added for coordinate regulation of amino acid metabolism, carbohydrate decomposition and lipid metabolism of the body, so body fatigue caused by acute exercises of the human body is regulated, the function of reservation of hepatic glycogen is improved, etc.

The invention relates to the field of biological analysis methods, and in particular to an analysis method of cecal metabolome in a broiler chicken. The change of metabolites and metabolic pathways ofcecal contents after the broiler chicken eats a yeast culture produced at different fermentation time is detected by jointly using a gas chromatography-mass spectrometry, thereby determining the influence of the yeast culture on the cecal metabolic regulation and the metabolites of the broiler chicken. Metabolomics data show that yeast culture is used for feeding the broiler chicken, the cecal differential metabolites mainly involve in amino acid metabolism and cholesterol metabolism, and participates in fat, vitamin digestion and absorption processes.

The invention provides acid-resistant and bile-salt-resistant lactobacillus crispatus. The strain has the preservation name of lactobacillus crispatus ZLC020. The preservation number is CGMCC No.11531. The strain disclosed by the invention has excellent anti-bacterium performance; the obvious bacteriostatic effect is achieved on swine escherichia coli. The strain, the relevant strain and relevantfeed can improve the production performance of growth pig; the intestinal flora composition is improved; the body amino acid metabolism is promoted; good development and application prospects are realized.

A method for alleviating at least one of the disturbance of bile acid metabolism, the disturbance of amino acid metabolism, and the disturbance of gut microbiota metabolism in a subject, comprising administering to the subject a composition or a health food comprising an active component selected from the group consisting of gallic acid, a pharmaceutically acceptable salt of gallic acid, a pharmaceutically acceptable ester of gallic acid, and combinations thereof.

A Yersinia pestis virulence regulator TyrR and applications thereof are provided. It is found that virulence of TyrR-deleted Yersinia pestis strains is lowered about 10000 times than virulence of wild strains, lack of the TyrR has no influence on in-vitro growth of the Yersinia pestis, and the TyrR can achieve negative regulation of transcription of five aromatic amino acid metabolism genes comprising aroF-tyrA, aroP, aroL and tyrP, and achieve positive regulation of two acid response genes which are hdeB and hdeD, so that the TyrR is proved to be a Yersinia pestis virulence regulator. In an environment having a pH value of 5.0, the survival rate and a growth speed of a Yersinia pestis TyrR mutant strain are lower than that of a wild strain, thus proving that the acid resistance of a TyrR mutant strain is lowered and that the TyrR regulator is closely related to acid resistance of the Yersinia pestis. Applications of the Yersinia pestis virulence regulator TyrR in preparation of anti-plague vaccines, and applications of the virulence regulator TyrR in preparation of medicines adopting the TyrR as a target are provided.

The invention discloses a method for analyzing the influence of taurine on the growth of tilapia on the basis of NMR metabonomics technology. The method comprises: collecting fish muscle samples and collecting the one-dimensional nuclear magnetic resonance hydrogen spectrum of each sample to obtain the original spectrogram of each sample; performing data preprocessing on each original spectrogramto obtain a two-dimensional data matrix; performing multivariate statistical analysis on the two-dimensional data matrix to obtain small molecular metabolites of each tilapia muscle tissue under the influence of different taurine addition amounts; and obtaining a metabolite change law through the identification and pattern recognition analysis of the metabolites, and screening biomarkers related to sugar metabolism, lipid metabolism and amino acid metabolism. According to the method for analyzing the influence of the taurine on the growth of the tilapia on the basis of the NMR metabonomics technology, the nuclear magnetic resonance metabonomics technology is used for analyzing the influence of the taurine on the growth of the tilapia, so that the metabolic difference of tilapia muscles under the influence of different taurine addition amounts can be rapidly, accurately, and comprehensively obtained, and the addition accuracy of taurine feed in tilapia breeding and the influence on thegrowth of the tilapia can be effectively evaluated.

The invention discloses a method used for measuring influences of long term applications of high and low nitrogen fertilizers on corn kernel albumin. According to the method, proteomic method is adopted to study long term positioned fertilization of high and low nitrogen fertilizers on summer corn Luyu 16 kernel albumin. It is shown by results that: the albumin content of a reference group is higher than those of two processed groups, and obvious difference strips are observed in quantificative unidirectional SDS-PAGE. It is shown by dimensional electrophoresis results that when a long term low nitrogen treated group is compared with the reference group, 7 differential protein spots are detected, one up-regulation is detected, and 6 down-regulation is detected; when a long term high nitrogen treated group is compared with the reference group, 6 differential protein spots are detected, one up-regulation is detected, and 5 down-regulation is detected. Mass spectrum identification and function classification are adopted, it is found that the proteins are a protein and amino acid metabolism associated protein, an energy metabolism associated protein, an expression regulation and control and defense and stress resistance associated protein, a cell metabolism associated protein, and a protein with unknown functions.

To provide an anti-cancer agent sensitivity determination marker, which marker can determine whether or not the patient has a therapeutic response to the anti-cancer agent, and novel cancer therapeutic means employing the marker.The anti-cancer agent sensitivity determination marker, the anti-cancer agent including oxaliplatin or a salt thereof and fluorouracil or a salt thereof, contains one or more substances selected from among an amino-acid-metabolism-related substance, a nucleic-acid-metabolism-related substance, a substance in the pentose phosphate pathway, a substance in the glycolytic pathway, a substance in the TCA cycle, a polyamine-metabolism-related substance, 7,8-dihydrobiopterin, 6-phosphogluconic acid, butyric acid, triethanolamine, 1-methylnicotinamide, NADH, NAD+, and a substance involved in the metabolism of any of these substances.

The invention relates to an amino acid metabolomics-based method for researching an anti-insomnia action mechanism of sugemule. Amino acid metabolome in each of tissues of the brains of para-chlorophenylalanine (PCPA) insomnic model rats before and after gavage with the sugemule is analyzed by applying a gas chromatography-mass spectrometer (GC-MS) method; endogenous compounds are identified by astandard fingerprint bank; changes of metabolite maps of all parts of the brains are analyzed and related metabolic markers are assayed by employing principal component analysis (PCA) and partial least squares-discrimination analysis (PLS-DA) modes; a targeting metabolomics research is conducted by applying liquid chromatograph-mass spectrometer (LC-MS) to explore content changes of neurotransmitters including 5-hydroxytryptamine, gamma-aminobutyric acid (GABA), glutamic acid (Glu) and the like in the brain tissues and related metabolites; by seeking for difference metabolites related with thecurative effect of the sugemule and metabolic pathways of the difference metabolites from brain tissue samples of clinical rats, the anti-insomnia action mechanism and an action site of the sugemuleare specified; and a metabolomics research means is applied for the first time by the amino acid metabolomics-based method to preliminarily confirm the action mechanism of the sugemule regulating andcontrolling a sleep-wake rhythm by a neurotransmitter system.

The invention provides a method for detecting the concentration of amino acid generated in the metabolic process of microorganisms based on a supramolecular fluorescence system and realizing high-throughput screening of the microorganisms by combining a droplet microfluidic technology. The method comprises the following steps of: (1) generating water-in-oil droplets on a micro-fluidic chip, and researching the influence of amino acid on the fluorescence intensity of a cucurbituril / fluorescent dye supramolecular system in the micro-fluidic droplets; (2) wrapping and culturing microorganisms byutilizing a droplet microfluidic technology, then injecting cucurbituril / fluorescent dye into microfluidic droplets, and distinguishing the microorganisms with different amino acid metabolic capabilities according to the change of the fluorescence intensity of the droplets; and (3) carrying out high-throughput screening on the microorganisms with different amino acid metabolic capabilities by utilizing a microfluidic droplet screening technology according to the difference of fluorescence intensity of each microfluidic droplet. Fluorescence of the supramolecular system is researched in the microfluidic droplets for the first time and applied to detection of the metabolite concentration of the microorganisms, and high-throughput screening of the microorganisms with different metabolic capabilities is established accordingly. The detection method has the advantages of high sensitivity, high speed and the like, with the screening flux reaching millions of droplets per hour. By adopting the high-throughput screening method disclosed by the invention, microorganisms capable of producing target products at high yield are obtained.

The invention discloses application of sodium dichloroacetate in preparation of a low protein feed for pigs. The inventor firstly classifies raw materials into three types; the first type is known as a micro-class raw material with the mixing proportion less than 2 percent, the second type is known as a medium-class raw material with the mixing proportion greater than 2 percent and less than 5 percent, and the third type is known as a large-class raw material with the mixing proportion greater than 5 percent. The low protein feed for the pigs is prepared by the following steps: firstly, mixing the first type of feed raw materials, fully and uniformly mixing the raw materials through a stirrer, then mixing the raw materials with the medium-class raw materials, thus obtaining a mixture, and finally, mixing the mixture with the large-class raw materials, thus obtaining the amino acid metabolism thrift-based low protein feed for the pigs; granulating the mixture according to the particle size of 8 to 12 mm; and transferring the particles into a drying machine for drying at the temperature of 95 to 105 DEG C, thus preparing a particulate feed finished product. The feed is wide in source, low in cost and comprehensive in nutrition; the level of coarse proteins in the feed can be obviously reduced; the growing performance of the animal cannot be affected; and the low protein feed is applicable to large-scale production.

The invention relates to an antibacterial drug sensitivity test detection method based on a fluorescent D-type amino acid metabolism marker. According to the method, the fluorescent D-type amino acidmetabolism marker is utilized to mark a bacterial solution obtained by mixing and incubating with an antibiotic solution, so that the minimum inhibitory concentration of antibiotics can be obtained according to the fluorescence signal intensity of the bacterial solution.

Provided is a marker that determines effectiveness prior to or in an early stage of disease treatment with a PD-1 signal inhibitor. A biomarker that can serve as an indicator of metabolic change related to T-cell mitochondrial activity and / or T-cell activation in a subject is used as a biomarker for predicting or determining the effectiveness of treatment with a PD-1 signal inhibitor. The following can be used as the indicator: intestinal flora-related metabolites in serum or plasma; energy metabolism-related metabolites in serum or plasma; amino acid metabolism-related metabolites and / or derivatives thereof in serum or plasma; oxygen consumption level and / or ATP turnover rate in peripheral CD8+ cells; amino acids in T-cells; and T-bet in peripheral CD8+ cells.

The invention discloses a protein-rich coarse cereal formula food for old people. The food is prepared from the following components in parts by weight: 25-35 parts of quinoa, 15-25 parts of oat, 8-15parts of buckwheat, 12-25 parts of hydrolyzed egg white powder, 10-18 parts of yolk powder, 1-3 parts of coarse cereal bran / hull, 0.02-0.08 part of multi-vitamin and 0.01-0.05 part of complex mineralsubstances. The nutritional formula food for old people, prepared from egg complex coarse cereals, has the characteristics of being high in digestion and absorption utilization ratio, capable of enhancing gastrointestinal peristalsis of old people and balancing nutrition and the like, and can promote amino acid metabolism balance of old people, inhibit muscle tissue degradation and prevent constipation, thereby overcoming a series of health problems caused by aging of the digestive system of old people.

The present invention relates methods for treating cancer by comprising administering to the subject an agent for reducing at least one NEAA, inhibiting the PPP pathway, inhibiting the sorbitol pathway, inhibiting heme biosynthesis, or any combination thereof. The invention also includes methods of treating cancer comprising detecting a tumor as having increased ROS administering to the subject an agent for reducing at least one NEAA, inhibiting the PPP pathway, inhibiting the sorbitol pathway or inhibiting heme biosynthesis, or any combination thereof.

Although metabolic networks have been reconstructed on a genome-scale, the corresponding reconstruction and integration of governing transcriptional regulatory networks has not been fully achieved. Here such an integrated network was constructed for amino acid metabolism in Escherichia coli. Analysis of ChlP-chip and gene expression data for the transcription factors ArgR, Lrp, and TrpR showed that 19 / 20 amino acid biosynthetic pathways are either directly or indirectly controlled by these regulators. Classifying the regulated genes into three functional categories of transport, biosynthesis, and metabolism leads to elucidation of regulatory motifs constituting the integrated network's basic building blocks. The regulatory logic of these motifs was determined based on the relationships between transcription factor binding and changes in transcript levels in response to exogenous amino acids. Remarkably, the resulting logic shows how amino acids are differentiated as signaling and nutrient molecules. This reveals the overarching regulatory principles of the amino acid stimulon.

The invention is directed to methods for treating disorders of amino acid metabolism, in particular, phenylketonuria (PKU). Such methods utilize novel compositions including Amnion-derived Multipotent Progenitor cells (herein referred to as AMP cells) alone or in combination with other agents or treatment modalities.

Methods and formulations of modified release amino acids are provided for the treatment or management of diseases defined by impaired amino acid metabolism, with improved pharmacokinetics, metabolism and utilization.

Provided is an optical measurement device which can measure the balance between the oxygen utilization and the oxygen supply in the metabolism of a test subject. An optical measurement device 20 includes a photodetector 22 which measures the amount of a plurality of biological substances contained in a test subject 31 by receiving incidence light R1 and R2 from the test subject 31, and a processing portion 23 which functions as an activation degree calculation portion calculating an activation degree of at least two metabolic pathways among energy metabolism, nucleic acid metabolism, amino acid metabolism, or lipid metabolism based on the amount of the plurality of biological substances detected by the photodetector 22.

The invention discloses a genetically engineered bacterium for producing L-arginine as well as its construction method and application. The present invention integrates the gene encoding carbamoyl phosphate synthase and the gene encoding L-arginine biosynthesis pathway enzyme in Escherichia coli, and is related to arginine in the arginine synthesis pathway and the entire amino acid metabolism network in Escherichia coli The metabolic flow analysis and reconstruction of the genetically engineered bacteria with clear genetic background, no plasmid, no mutagenesis, and stable and efficient production of L-arginine were obtained, which has good L-arginine production ability.

The invention relates to the field of disease related gene mutation, especially gene mutation of autosomal recessive hereditary amino acid metabolism disease, maple syrup urine disease related gene mutation, and more particularly maple syrup urine disease related gene mutation, and a detection method and purpose thereof.

The invention discloses an application of an amino acid composition to a medicine for treating fetal nervous system development diseases, including a pharmaceutical application of the amino acid composition. The amino acid composition is used for preparing the medicine composition for preventing and treating the fetal nervous system development diseases. The invention further discloses an amino acid composition identification and statistical processing method. Amino acid metabolism characteristics of fetal amniotic fluid with abnormal central nervous system isolated soft indexes obtained by ultrasonic guided amniotic cavity puncture are studied to obtain the pharmaceutical composition for preventing and treating fetal nervous system development diseases, so that the diseases of simple ventricular plexus cyst and simple lateral ventricular dilatation of a fetus are treated, and the development and perfection of a fetal cranial nervous system are promoted.

The invention provides a mature wheat embryo callus induction medium. An MS medium is used as a basic medium, and is added with acetosyringone with the final concentration of 50-100 mu M / L, 1.0-2.0 mg / L of 2,4-D and an amino acid ingredient to form the mature wheat embryo callus induction medium, and the amino acid ingredient consists of glycine, aspartic acid, proline and leucine. The invention further provides application of the medium and a method for inducing a mature wheat embryo to form a callus. The medium has high inductivity to the mature wheat embryo callus, comprises simple components and is low in preparation cost. The mature wheat embryo callus induction medium lays a good theoretical basis on high-efficiency tissue regeneration of wheat and research on amino acid metabolism, and is good in application prospect.