3 results about "Acetosyringone" patented technology

This invention provides a method for transient transformation of oil palm zygote embryos based on Agrobacterium-mediated transformation, which employs the Agrobacterium-mediated approach and is based on the optimal Agrobacterium concentration (OD) for stable transformation of oil palm. 600 The optimal Agrobacterium infection time for zygotic embryos was determined by measuring the concentration of acetylsuccinone and co-culture time. The transient GUS expression staining rate was compared between zygotic embryos of different oil palm varieties and at different growth stages. The method described in this invention optimizes infection conditions based on stable transformation parameters, clarifies the optimal explant materials and treatment process, and effectively improves transient transformation efficiency and experimental repeatability. This study establishes for the first time an Agrobacterium-mediated transient transformation system for oil palm zygotic embryos and obtains zygotic embryos most suitable for transient transformation, achieving efficient transient expression of exogenous genes in oil palm zygotic embryos. This provides a rapid and low-cost technical means for studying oil palm gene function.

The application relates to the field of plant genetic engineering, and specifically discloses a high-efficiency gene transformation system based on the stem tip meristem of a yellowing seedling of Zhongmian 113. The core components of the system include L1 medium suitable for Zhongmian 113, Agrobacterium GV3101 carrying a target gene, a special resuspension solution containing acetosyringone and Silwet-L77, and special primers for overexpression and gene editing; and the preparation method is as follows: seed disinfection and germination are performed to obtain a yellowing seedling, the stem tip is cut in an X shape to expose the meristem, Agrobacterium infection is performed, and then dark culture and light culture are performed, and after transplanting and cultivation to the true leaf stage, positive plants are screened and identified through PCR; the system can be used for gene transformation of Zhongmian 113 and improvement of high yield, high quality, disease resistance and the like, and has the advantages of strong adaptability, high transformation efficiency and short cycle; in addition, the preparation method of the application does not need callus regeneration, is simple and convenient to operate, has a standardized process, is good in repeatability and stability, and can quickly realize transgenic seed acquisition.

The application belongs to the field of biology and modern agricultural technology, and discloses a rooting aid invasion agent, and a formula thereof is as follows: B5 culture medium 3.0-3.5 g / L, sucrose 28-32 g / L, MES 3.8-4.0 g / L, acetyl-syringone 38-42 mg / L, 6-BA 1.5-1.8 mg / L, gibberellin 0.02-0.03 mg / L, and surfactant Silwet L-77 100 ml / L, and pH is 5.4. The application also discloses a soybean hairy root induction and transformation method mediated by Agrobacterium rhizogenes by using the rooting aid invasion agent, and the method comprises the following steps: disinfection and germination of soybean seeds; resuspension of Agrobacterium rhizogenes with a target expression plasmid in the rooting aid invasion agent to obtain an Agrobacterium rhizogenes aid invasion liquid; infection; co-culture; and screening of positive roots.