Method used for measuring influences of long term applications of high and low nitrogen fertilizers on corn kernel albumin
A test method, the technology of albumin, is applied in the test field of long-term application of high and low nitrogen fertilizers on the effect of corn grain albumin, and can solve the problem that the study of summer corn grain albumin proteomics is still blank.
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[0028] The technical solutions of the present invention will be further described in detail below in conjunction with the accompanying drawings and specific embodiments.
[0029] 1 Materials and methods
[0030] 1.1 Experimental design
[0031] The experiment started in 1978 at the Laiyang Experimental Station of Qingdao Agricultural University. The soil for testing is non-calcareous fluvo-aquic soil, developed in alluvial parent material, topsoil texture is light loam, organic matter content is 4.10g / kg, total nitrogen content is 0.50g / kg, total phosphorus (P 2 o 5 ) amount is 0.46g / kg, soil available phosphorus (P 2 o 5 ) is 15mg / kg, soil available potassium (K 2 O) is 38mg / kg, and the soil cation replacement capacity is 11.80cmol / kg. A total of 3 treatments (control group, low nitrogen, high nitrogen) were set up in the experiment, and each treatment was repeated 3 times. The experimental site implements a winter wheat-summer corn rotation system, with two crops per ...
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