29627results about "Preparing sample for investigation" patented technology

The present disclosure relates to compositions and methods for reversible fixation of biological samples using fixation reagents that form bis-carbamate crosslinks between amine-bearing moieties in biomolecules.

A substrate holder includes a base configured to receive a substrate; a cover configured to mateably engage with the base, the cover defining an opening formed by inner sidewalls; and a removable insert defining a surface, the removable insert being configured to be received within the opening of the cover. The removable insert includes a gasket; a projection coupled to the gasket; and at least two insert tabs extending from opposite sides of the removable insert, each insert tab being configured to engage with at least one of the inner sidewalls forming the opening of the cover.

Provided herein are methods, compositions, and kits for the spatial analysis of target nucleic acids, or complements thereof, by their 5′ end.

Provided herein are method for determining a location of nucleic acids in fixed biological samples, in which the method includes use of a template switching oligonucleotide (TSO) and a randomer during second strand synthesis.

Systems and methods are provided for targeting one or more features in a region of interest, and efficiently removing the features from an array of features. The systems and methods can remove one or more beads that contain analytes, intermediate agents, or a combination thereof, from the region of interest of a substrate by emitting light toward the beads on the substrate.

The invention provides a preparation method of a transmission electron microscope ultrathin sample with a cross section of an electron beam sensitive material, which comprises the following steps: selecting a specific region as a to-be-extracted sample region for the electron beam sensitive material by adopting an electron beam imaging technology; depositing a protective layer on the surface of the to-be-extracted sample area by adopting an electron beam and / or an ion beam; bombarding a to-be-extracted sample area by utilizing a high-energy ion beam, extracting an electron beam sensitive material cross section sample from the to-be-extracted sample area, and fixing the extracted sample to a metal bracket; performing pre-thinning treatment on the extracted sample by using a focused ion beam to obtain a first thinned sample of which the thickness is reduced to be less than a first thickness; and transferring the first thinned sample into a micro-beam fixed-point ion thinning system, and carrying out multi-time stepped low-energy ion beam fine thinning treatment on the first thinned sample by using inert gas ion micro-beams to obtain a second thinned sample which is clean in surface and free of a surface ion damage layer and has the thickness reduced to be less than a second thickness, and taking the second thinned sample as a transmission electron microscope ultrathin sample.

Provided herein are methods for analyzing a biological sample that include contacting the biological sample with a non-conductive substrate; and contacting a matrix to a surface of the biological sample. Further methods optionally include performing mass spectrometry analysis; determining presence of a first analyte in the biological sample; and / or analyzing a second analyte on the surface of the biological sample to determine presence of the first and the second analyte in the biological sample.

The invention discloses a columnar transparent part defect detection method, and relates to the technical field of optical detection and machine vision, and the detection method comprises a detection preparation stage, a detection pose configuration stage, a structured light stripe excitation stage, a machine vision detection stage, a model optimization evaluation stage, and a detection post-processing stage. According to the defect detection method for the columnar transparent part, the comprehensive performance of defect detection of the columnar transparent part is remarkably improved through the synergistic effect of coaxial pose optimization, composite structure light source design and improved YOLOv8 deep learning model; various common surface and subsurface defects in production are covered; the real-time detection requirement of an industrial production line is met through relative movement of the part and the light source and high-speed image acquisition and processing; the system has strong anti-interference capability, and can adapt to detection scenes of different materials, sizes and environmental conditions.

The invention belongs to the related technical field of microbiological treatment of soil, and discloses a method for improving microbiological sand consolidation strength based on modified plant fibers. Natural plant fibers are subjected to alkali treatment modification, so that non-cellulose components on the surfaces of the natural plant fibers are removed, the roughness and the specific surface area are remarkably improved, the interface friction force and the mechanical connection capacity between the fibers and sand particles are enhanced, more nucleation sites are provided for microorganisms to induce calcium carbonate precipitation, and the content of calcium carbonate is increased. The strength of the solidified sandy soil can be effectively improved, and the biomineralization period is greatly shortened. Meanwhile, after the modified fibers are doped into the sandy soil, a three-dimensional interlaced fiber network structure can be formed in the material in combination with a microorganism induced calcium carbonate deposition (MICP) technology, and the cohesiveness among sand grains and the mechanical property of the overall structure are effectively improved. According to the solidification method, the solidification strength of the sand column is remarkably improved, the technology is simple, green and environment-friendly, and good engineering adaptability and popularization value are achieved in the field of sand solidification.

The invention relates to the technical field of concrete testing, in particular to a concrete testing method which comprises the following steps: manufacturing a plurality of groups of standard-size concrete test pieces according to a preset concrete mix proportion, and respectively placing the standard-size concrete test pieces under a simulated curing condition and a standard curing condition for curing in a preset curing period to form a first test group and a first control group; based on a plurality of detection points, carrying out a rebound test and an ultrasonic test on the concrete test pieces in the first test group, and determining a high-frequency ultrasonic section to determine an ultrasonic attenuation coefficient of a single concrete test piece, according to the ultrasonic propagation speed, the ultrasonic attenuation coefficient and the detection rebound value, constructing a concrete strength-based correction model to obtain the comprehensive compressive strength of the concrete test pieces in the first test group, and determining the standard compressive strength corresponding to the concrete test pieces in the first control group, and determining an initial influence coefficient of the simulated maintenance condition according to the comprehensive compressive strength and the standard compressive strength. The compressive strength of the concrete structure is effectively tested.

The invention provides a mineral leaching property analysis method, which belongs to the field of process mineralogy, and comprises the following steps: crushing and enriching a sample to be detected, and selecting single mineral particles to obtain target mineral particles; the preparation method comprises the following steps: coating a sample preparation grinding tool with epoxy resin glue, adding target mineral particles, and carrying out curing and grinding treatment to obtain a primary sample; cutting the primary sample, placing the grinding surface downwards and the three wear-resistant alloys at the bottom of a sample preparation grinding tool, injecting epoxy resin glue, curing to form a composite sample, accurately grinding, polishing, performing carbon spraying treatment, performing first automatic mineralogical analysis, measuring the area of a target mineral, cleaning, and performing leaching treatment to obtain a treated sample; and after solidification, accurate grinding and polishing and carbon spraying treatment, secondary automatic mineralogical analysis is carried out, the area of the residual target mineral is measured, and the leaching index of the target mineral is calculated. Reliable technical support is provided for mineral processing technology optimization and resource evaluation, and the method is particularly suitable for development of efficient leaching technologies of precious metal, rare earth and the like.

The invention relates to the field of performance testing of road engineering materials, and discloses an emulsified asphalt in-situ cold-recycled mixture high-temperature creep test method which comprises the following steps: preparing a test piece: presetting an old material-emulsified asphalt standard test piece, and embedding a moisture regulation and control device to generate a controllable gradient; multi-field loading: coupling an unsteady-state temperature field, a moisture gradient field and a time-varying attenuation stress field to construct a composite loading environment; synchronously aligning data: synchronously acquiring macro strain and micropore data, and realizing cross-scale alignment through a space-time matrix; performing parameter inversion: inverting nonlinear constitutive parameters by combining macro and micro data, and constraining a solution space by using a thermodynamic equation; and intelligent termination output: triggering a termination mechanism based on a multi-dimensional criterion and outputting damage evolution data. Through multi-field coupling loading, cross-scale data fusion and intelligent criterion cooperation, the problems of traditional test environment distortion, parameter deviation and criterion lag are solved, and accurate analysis of the high-temperature creep behavior of the emulsified asphalt reclaimed material and reliable prediction of the performance degradation law are achieved.

The invention provides a method and system for detecting the content of platinum, palladium and rhodium in an automobile exhaust purification catalyst and application, and relates to the technical field of automobile exhaust purification, the method comprises the following steps: crushing, grinding, sieving, performing microwave acidolysis, neutralizing, washing and drying a catalyst sample to obtain dry filter residues; a trapping agent and a fluxing agent are added into the dry filter residues, constant-temperature heating is conducted in inert gas, then cooling forming is conducted, and an alloy button is obtained; blowing ash on the alloy, then adding mixed acid into the alloy, heating the alloy in a water bath at 95 DEG C for 25-35 minutes to obtain a dissolved solution, then adding a masking agent into the dissolved solution, and oscillating to obtain a solution to be detected; and filtering the liquid to be detected, fixing the volume and detecting. According to the method, precise quantification of trace platinum, palladium and rhodium in the complex matrix catalyst can be realized, and the method has a relatively high recovery rate; meanwhile, pollution elements are prevented from being used, and the method is green and efficient and better conforms to the concept of green chemistry.

The invention provides a method for detecting the porosity of ores in a dump leaching process, and belongs to the technical field of detection of the porosity of the ores. The method comprises the following steps: taking a dump leaching ore body, screening, uniformly mixing, splitting, putting into a dump leaching column to obtain the volume of a sample subjected to splitting in the dump leaching column, then performing fine grinding, putting into the dump leaching column to obtain the volume of the sample subjected to fine grinding in the dump leaching column, and then performing image analysis on the sample subjected to fine grinding to obtain the pore volume among sample particles subjected to fine grinding. And finally, converting the three volumes to obtain the porosity of the heap-leached ore. Through screening treatment, the sample representativeness is effectively improved; the ore before superfine grinding and after superfine grinding is introduced into a dump leaching column, the sampling uniformity is controlled, and the accuracy and stability of data are improved through the in-pile process under the same test column; an image analysis algorithm is introduced, and meanwhile, a correction coefficient is introduced, so that small pores among particles are precisely measured and calculated. The method is simple in process and convenient to operate, and the ore porosity can be rapidly and accurately measured and calculated.

The invention relates to the technical field of analysis of physical and chemical properties of soil, in particular to a dry farming soil fertility rapid detection and fertility improvement method, which comprises the following steps: sorting a colloidal phase and a dissolved phase of a humic acid complex based on density difference and dielectric property difference through a gradient centrifugation and dielectrophoresis enriched physical field synergistic separation technology; a two-channel detection time sequence is optimized by adopting a genetic algorithm, a non-overlapping time sequence signal flow is generated by taking a signal interference suppression ratio as a fitness function, and time domain cross interference is suppressed. A programmable resistance-capacitance compensation model is constructed, parallel capacitor array parameters are dynamically adjusted to correct electrochemical phase angle offset, and a three-dimensional fertility distribution curved surface and a residual map are generated in combination with a Kriging interpolation model. On the basis of residual spectrum spatial distribution characteristics, a cross-linked slow-release system is deployed to improve the organic matter binding capacity of a humic acid deficiency area, a mesoporous zeolite-based passivator is applied to regulate and control an ion migration path, a closed-loop feedback mechanism is formed through dynamic phase response monitoring, and accurate detection and directional remediation of soil fertility are achieved.

A sample preparation instrument with an integrated device for estimating a concentration of white blood cells in a specimen is described. The sample preparation instrument receives a specimen for which a sample is to be prepared for analysis. The integrated device can implement an optical method, an electrical resistance method, or a flow cytometry method, for example, to estimate the white blood cell concentration in the specimen. For some types of analysis, a sample volume of the specimen is dependent on the white blood cell concentration. Therefore, the sample preparation instrument can automatically determine and adjust the sample volume of the specimen based on the estimated white blood cell concentration prior to adding additional reagents, such as labeling reagents and / or lytic reagents, to prepare the sample.

The invention provides a coal impact tendency evaluation method which comprises the following steps: sampling a coal rock material on an engineering site, and processing the sample into a coal rock test piece with a preset size and a preset shape; carrying out a uniaxial compression experiment on the coal rock test piece, and recording a motion image of an ejection body on the coal rock test piece in the loading process of the coal rock test piece; comparing cosine similarities among the plurality of images in the moving images, and determining the image with the maximum damage degree according to the cosine similarities; analyzing gray level distribution of the image with the maximum damage degree to obtain a gray level histogram; identifying particle information of the broken particles in the image with the maximum damage degree; representing the damage degree of the coal rock material by using the particle information of the crushed particles; and evaluating the coal impact tendency by using the damage degree of the coal rock material. According to the evaluation method for the coal impact tendency disclosed by the invention, a new coal impact tendency evaluation index is provided, the research on the damage process of the coal rock material is perfected, and basic parameters are provided for subsequent early warning of damage of the coal rock material.

The invention provides a plywood weather resistance accelerated aging test device and test method, and belongs to the technical field of aging test equipment. The testing device comprises a supporting table and a testing box fixedly installed at the top of the supporting table, a rotary supporting mechanism is arranged in the testing box and used for positioning the plywood and adjusting the rotation angle of the plywood in the horizontal plane, and two waxing mechanisms are further arranged in the testing box and used for waxing the plywood in the horizontal plane. The two waxing mechanisms are used for conducting edge sealing treatment on the plywood. The testing method comprises the following steps: T2, placing the plywood on a positioning carrier plate on the rotary supporting mechanism to complete positioning; and T3, the plywood is fixed through a pressing mechanism. The plywood weatherability accelerated aging test device provided by the invention has the advantages that the plywood is automatically subjected to edge sealing and waxing, the human input can be reduced, and the reliability and integrity of wax layer coating can be improved.

The invention provides a method for detecting the porosity of in-situ dump-leached ore, and belongs to the technical field of ore porosity detection. The method comprises the following steps: taking an ore body on site, preparing a filling agent, performing vacuum gradient impregnation, curing, dissolving and separating the ore to obtain a pore model; melting and softening the pore model to obtain the pore volume in the ore, and finally obtaining the porosity of the ore; wherein nano SiO2 is added into the filler resin, and permeation of tiny pores is guaranteed based on the size effect and the interface optimization effect of a nano material; and through vacuum gradient impregnation, sufficient permeation of tiny pores is further ensured. According to the detection method, the limitation of a drainage method on ore pores containing water-soluble minerals is avoided; the problems that ore samples need to be collected and taken back to a laboratory to be detected in a mercury injection method, a gas adsorption method and the like, the ore body structure is damaged, and consumed time is long are solved. Detection can be carried out on site, ore permeability parameters are provided for the in-situ dump leaching process, and a real-time basis is provided for flow selection.

The invention discloses a standard volume-based mass spectrum signal calibration system and method, and belongs to the field of measurement, the standard volume-based mass spectrum signal calibration system comprises a standard flow generation system and a mass spectrum detection system.The standard flow generation system comprises a standard tank, a first valve, a fifth valve and a micro-leakage valve which are sequentially connected through a gas pipeline; a gas inlet pipeline, a film gauge and a first molecular pump set are connected to a gas pipeline connecting the first valve and the fifth valve in parallel, a second valve is installed on the gas inlet pipeline, and a fourth valve is installed at an inlet of the first molecular pump set. The mass spectrometry detection system comprises a vacuum chamber communicated with the micro-leakage valve through a gas pipeline, a mass spectrometer, a second molecular pump set and a full-scale gauge are connected to the vacuum chamber, and a gate valve is installed at an inlet of the second molecular pump set. The gas release rate can be calculated and continuously adjusted on the premise that a leak hole is not needed, and the problems that in the prior art, a standard leak hole is limited in application range, low-flux measurement is inaccurate, the nominal leak rate is prone to drifting, and cost is high are solved.

The invention provides an ore leaching property analysis method, and belongs to the field of process mineralogy, and the method comprises the following steps: taking a to-be-detected sample, carrying out ore grinding treatment, and carrying out ore washing and desliming to obtain a pretreated sample; mixing the pretreated sample with graphite to prepare an automatic mineralogical analysis sample, measuring the content of a target mineral and the content of a target element through automatic mineralogical analysis, and calculating the average content of the target mineral; adding an agent into the pretreated sample to carry out leaching reaction, carrying out solid-liquid separation to obtain mineral residues, and calculating the mass ratio of the mineral residues to the pretreated sample before reaction; mixing mineral residues with graphite to prepare an automatic mineralogical analysis sample, analyzing the sample, measuring the content of residual target minerals and the content of residual target elements, and calculating the average content of the residual target minerals; and calculating a mineral leaching coefficient and an ore leaching coefficient based on the obtained data. The in-situ leaching recovery rate prediction method based on mineralogical analysis is suitable for development and evaluation of low-grade and complex ore bodies.

The invention provides a harmful organic matter infrared spectrum detection method based on segmented multi-dimensional harmonic decomposition, relates to the technical field of infrared spectrum analysis, and aims to solve the problems of noise interference, peak overlapping and inaccurate signal extraction during harmful organic matter detection in the prior art. Obtaining a sample solution to be detected and obtaining an original infrared spectrum of the sample solution to be detected; the method comprises the following steps of: segmenting a dynamic wave band, and performing local signal decomposition on each segment by adopting a harmonic primary function with a proper order; the absorption peak of the target harmful organic compound is accurately extracted by utilizing wave band coupling and characteristic peak identification; stable and accurate quantitative analysis is realized by combining partial least squares, so that the detection sensitivity and accuracy are improved, and the safety of food contact materials is ensured; according to the method, the accuracy and reliability of signal denoising, peak identification and quantitative analysis under a complex matrix are effectively improved by adopting a wave band segmentation strategy, segmented multi-dimensional harmonic decomposition and wave band coupling and characteristic peak identification.

Systems and methods are provided for targeting one or more features in a region of interest, and efficiently removing the features from an array of features. The systems and methods can remove one or more beads that contain analytes, intermediate agents, or a combination thereof, from the region of interest of a substrate by emitting light toward the beads on the substrate.

The invention discloses a cement stabilized macadam mechanical property analysis method based on maximum aggregate nominal particle size, and relates to the technical field of cement stabilized macadam mechanical property analysis, the method comprises the following steps: preparing skeleton dense type cement stabilized macadam test pieces with different nominal maximum particle sizes; carrying out a macroscopic mechanical test to obtain a stress-strain curve and a crushing value; establishing a three-dimensional discrete element numerical model corresponding to the test piece, performing uniaxial compression simulation, and ensuring the reliability through model verification; and based on the verified model, quantitatively analyzing the contact force distribution, the contact quantity characteristic and the microcrack evolution law from the microscopic level. According to the analysis method disclosed by the invention, a mechanism that the nominal maximum particle size improves the macroscopic performance of the material by regulating and controlling an internal force chain network and inhibiting damage accumulation is disclosed through a macro-micro combination means, and a scientific micro-mechanical basis and a quantitative analysis tool are provided for grading optimization and high-performance design of the cement stabilized macadam base.

The invention provides a method for detecting silicon single crystal COP free area distribution, and relates to the technical field of silicon single crystal quality detection.The method comprises the steps that a silicon wafer to be detected is cleaned to obtain a first silicon wafer; uniformly smearing a transition metal ion solution on the surface of the first silicon wafer to obtain a second silicon wafer; placing the second silicon wafer in a high-temperature environment, and performing heat treatment under three temperature gradients to obtain a third silicon wafer; wherein in the first stage, transition metal ions are diffused to the surface layer of the silicon wafer to activate oxygen precipitates in a Pv region, in the second stage, the transition metal ions are promoted to be diffused into the silicon wafer to develop a Pi low-oxygen region, and in the third stage, nuclei coalescence in a B-band region is activated at high temperature to form a differential arrangement ring; cleaning the third silicon wafer through a mixed acid solution to obtain a fourth silicon wafer; and placing the fourth silicon wafer in a preferential corrosion liquid for preferential corrosion, and carrying out COP free area distribution detection on the obtained detection sample wafer. According to the scheme, the definition of each region boundary can be improved, so that the detection accuracy of COP free region distribution is improved.

The present disclosure relates to systems and methods for facing a tissue block. In some embodiments, a method is provided for facing a tissue block that includes imaging a tissue block to generate imaging data of the tissue block, the tissue block comprising a tissue sample embedded in an embedding material, estimating, based on the imaging data, a depth profile of the tissue block, wherein the depth profile comprises a thickness of the embedding material to be removed to expose the tissue sample to a pre-determined criteria, and removing the thickness of the embedding material to expose the tissue to the pre-determined criteria.

The invention relates to the technical field of water quality detection, in particular to garden water quality monitoring equipment which comprises an outer shell, a supporting disc is arranged in the outer shell, and multiple sets of test tubes are detachably installed on the supporting disc; the lifting assembly is connected with a support arranged in the outer shell, and the lifting assembly can drive the bearing disc to move in the vertical direction of the space; the power assembly is connected with the bearing disc and the lifting assembly, and the power assembly can drive the bearing disc and the lifting assembly to act alternately; the pressure applying structure is arranged on the bracket, and the pressure applying structure can keep the test tube in a static state when the bearing disc moves upwards; the reagent adding device is mounted on the bracket, and a plurality of groups of liquid outlets extending to the inner side of the pressure applying structure are formed in the reagent adding device; and the folding assembly is connected with the liquid outlets, and can drive the multiple groups of liquid outlets to move close to each other after the bearing disc ascends to a preset position so as to abut against the side wall of the test tube, so that the detection speed is increased, and liquid splashing is prevented.

The utility model discloses a tensile shear and stripping test piece sample preparation tool, which belongs to the field of adhesive detection and comprises a bottom plate, a tensile shear sample preparation component and a stripping sample preparation component, and the tensile shear sample preparation component and the stripping sample preparation component are arranged on the bottom plate. The tensile shear sample preparation assembly comprises a first test piece groove and a second test piece groove which are formed in the bottom plate, and the first test piece groove and the second test piece groove are formed in the first direction; the stripping sample preparation assembly comprises two guide stand columns arranged on the bottom plate, a plurality of pressing blocks are arranged between the two guide stand columns in a sliding mode in the second direction, and test piece mounting grooves are formed in the pressing blocks in the third direction; the first test piece groove and the second test piece groove are located between the two guide stand columns, the pressing block is located above the second test piece groove, and the first direction, the second direction and the third direction are perpendicular to one another. The device can be used for simultaneously preparing a tensile shear test piece and a stripping test piece, and the adhesive layer thicknesses of different adhesives can meet the standard requirements by adjusting the pressure.

An automated tape transfer apparatus including a tape feed mechanism feeding a continuous length of an adhesive tape through the automated tape transfer apparatus and a tape applicator applying the adhesive tape to a cutting face of a sample block, wherein a section of the sample block is adhered to the adhesive tape after cutting of the section from the sample block. A slide station transfers the cut section from the adhesive tape to a slide.

The invention belongs to the field of atmospheric environment particulate matter monitoring, and particularly relates to an atmospheric environment salt mist concentration real-time monitoring device and method. The monitoring device comprises a computer control module, a container and a buoy, a conductivity electrode, a temperature sensor, a displacement sensor, an air inlet pipe and an air outlet pipe are integrated on the top of the container, and the air outlet pipe is connected with a reciprocating cylinder with a piston and a limit switch. The monitoring method comprises the following steps: calibrating the initial volume V < initial > of liquid and the volume V < initial > of a reciprocating cylinder; circularly collecting the conductivity k0 and k1, the temperature T0 and T1 and the displacement D and Dtotal of the buoy before and after ventilation; correcting the salt mass increment delta M through the dynamic volume, and finally outputting the salt mist concentration C; according to the invention, the defects of inaccurate ventilation volume metering, solution density change and interference of moisture in high-humidity air on concentration monitoring in the prior art are overcome, full-automatic continuous monitoring of the salt mist concentration is realized, the measurement error is effectively reduced, the measurement precision and the monitoring capability are remarkably improved, and the method has relatively good environmental adaptability.