42257results about "Preparing sample for investigation" patented technology

The present disclosure provides compositions and methods for using fixed biological samples in partition-based assays. In at least one embodiment, the disclosure provides a composition comprising a fixed biological sample and an un-fixing agent contained in a partition, such as a discrete droplet. In some embodiments, the disclosure provides un-fixing agent compounds capable of catalytically cleaving crosslinks in fixed biological samples, particularly crosslinked nucleic acids, such as RNA.

Provided herein are methods, compositions, and kits for the spatial analysis of target nucleic acids, or complements thereof, by their 5′ end.

A substrate holder includes a base configured to receive a substrate; a cover configured to mateably engage with the base, the cover defining an opening formed by inner sidewalls; and a removable insert defining a surface, the removable insert being configured to be received within the opening of the cover. The removable insert includes a gasket; a projection coupled to the gasket; and at least two insert tabs extending from opposite sides of the removable insert, each insert tab being configured to engage with at least one of the inner sidewalls forming the opening of the cover.

Provided herein are methods for preparing biological samples for spatial proteomic analysis, methods of determining a location of a protein analyte in a biological sample, and methods of determining a location of a protein analyte and a nucleic acid analyte in a biological sample.

Localized detection of RNA in a tissue sample that includes cells is accomplished on an array. The array include a number of features on a substrate. Each feature includes a different capture probe immobilized such that the capture probe has a free 3′ end. Each feature occupies a distinct position on the array and has an area of less than about 1 mm2. Each capture probe is a nucleic acid molecule, which includes a positional domain including a nucleotide sequence unique to a particular feature, and a capture domain including a nucleotide sequence complementary to the RNA to be detected. The capture domain can be at a position 3′ of the positional domain.

Localized detection of RNA in a tissue sample that includes cells is accomplished on an array. The array include a number of features on a substrate. Each feature includes a different capture probe immobilized such that the capture probe has a free 3′ end. Each feature occupies a distinct position on the array and has an area of less than about 1 mm2. Each capture probe is a nucleic acid molecule, which includes a positional domain including a nucleotide sequence unique to a particular feature, and a capture domain including a nucleotide sequence complementary to the RNA to be detected. The capture domain can be at a position 3′ of the positional domain.

A method for aligning a sample to an array is provided. An image of sample image of a sample can be received by a data processor. The sample image having a first resolution. An array image including an overlay of an array with the sample and an array fiducial can be received by the data processor. The array image having a second resolution lower than the first resolution of the sample image. The sample image can be regisered to the array image by aligning the sample image and the array image. An aligned image can be generated based on the registering. The aligned image can can include an overlay of the sample image with the array. The aligned image can be provided by the data processor. A method for detecing fiducials associated with an array is provided. Systems and non-transitory computer readable mediums performing the method are also provided.

The present disclosure relates to compositions and methods for reversible fixation of biological samples using fixation reagents that form bis-carbamate crosslinks between amine-bearing moieties in biomolecules.

A substrate holder includes a base configured to receive a substrate; a cover configured to mateably engage with the base, the cover defining an opening formed by inner sidewalls; and a removable insert defining a surface, the removable insert being configured to be received within the opening of the cover. The removable insert includes a gasket; a projection coupled to the gasket; and at least two insert tabs extending from opposite sides of the removable insert, each insert tab being configured to engage with at least one of the inner sidewalls forming the opening of the cover.

Provided herein are methods, compositions, and kits for the spatial analysis of target nucleic acids, or complements thereof, by their 5′ end.

Provided herein are method for determining a location of nucleic acids in fixed biological samples, in which the method includes use of a template switching oligonucleotide (TSO) and a randomer during second strand synthesis.

Systems and methods are provided for targeting one or more features in a region of interest, and efficiently removing the features from an array of features. The systems and methods can remove one or more beads that contain analytes, intermediate agents, or a combination thereof, from the region of interest of a substrate by emitting light toward the beads on the substrate.

The invention relates to the technical field of tea leaf detection, and discloses a tea leaf quality grade detection method and system.The method comprises the steps that a tea leaf sample is collected and pretreated; extracting chemical component indexes and physical properties of the pretreated tea leaves, and performing fusion and analysis; and establishing a tea quality grade classification model, inputting characteristics of a tea sample to be detected, outputting a detection result, and generating a tea quality grade report. Through combination of the support vector machine, the gradient boosting decision tree and the improved logistic regression algorithm, the precision of the tea quality classification model is optimized. The dynamic threshold function and the segmented excitation function are introduced to further solve the boundary fuzzy problem of the high-grade tea leaves and the low-grade tea leaves, and accurate judgment of tea leaf grade classification is achieved. And data restoration is carried out through weighted compensation correction and a recheck process, so that the output tea grade report is ensured to have high accuracy and reliability.

The invention provides a method for measuring the content of metal sulfide, and belongs to the technical field of process mineralogy. The method for measuring the content of the metal sulfide comprises the following steps: grinding ore, analyzing a sulfur phase, carrying out reselection and acid treatment on a sample subjected to ore grinding, respectively carrying out sulfur phase analysis and automatic mineralogy analysis on the sample subjected to acid treatment, and finally carrying out test analysis to obtain the content of the metal sulfide. According to the method, sulfur phase analysis is performed on the samples subjected to ore grinding and reselection and acid treatment enrichment, automatic mineralogical analysis is performed on the samples subjected to reselection and acid treatment enrichment, amplified data is corrected into data of original samples, accurate measurement of the content of the low-content metal sulfide is achieved, and the accuracy of measurement of the content of the low-content metal sulfide is improved. And through automatic mineralogical analysis, the contents of different metal sulfides can be respectively measured. The test method provided by the invention is accurate in data measurement, reduces interference of human subjective factors to a great extent, and provides reliable data support for efficient utilization of stone resources.

The invention provides a preparation method of a transmission electron microscope ultrathin sample with a cross section of an electron beam sensitive material, which comprises the following steps: selecting a specific region as a to-be-extracted sample region for the electron beam sensitive material by adopting an electron beam imaging technology; depositing a protective layer on the surface of the to-be-extracted sample area by adopting an electron beam and / or an ion beam; bombarding a to-be-extracted sample area by utilizing a high-energy ion beam, extracting an electron beam sensitive material cross section sample from the to-be-extracted sample area, and fixing the extracted sample to a metal bracket; performing pre-thinning treatment on the extracted sample by using a focused ion beam to obtain a first thinned sample of which the thickness is reduced to be less than a first thickness; and transferring the first thinned sample into a micro-beam fixed-point ion thinning system, and carrying out multi-time stepped low-energy ion beam fine thinning treatment on the first thinned sample by using inert gas ion micro-beams to obtain a second thinned sample which is clean in surface and free of a surface ion damage layer and has the thickness reduced to be less than a second thickness, and taking the second thinned sample as a transmission electron microscope ultrathin sample.

Provided herein are methods for analyzing a biological sample that include contacting the biological sample with a non-conductive substrate; and contacting a matrix to a surface of the biological sample. Further methods optionally include performing mass spectrometry analysis; determining presence of a first analyte in the biological sample; and / or analyzing a second analyte on the surface of the biological sample to determine presence of the first and the second analyte in the biological sample.

The invention discloses a columnar transparent part defect detection method, and relates to the technical field of optical detection and machine vision, and the detection method comprises a detection preparation stage, a detection pose configuration stage, a structured light stripe excitation stage, a machine vision detection stage, a model optimization evaluation stage, and a detection post-processing stage. According to the defect detection method for the columnar transparent part, the comprehensive performance of defect detection of the columnar transparent part is remarkably improved through the synergistic effect of coaxial pose optimization, composite structure light source design and improved YOLOv8 deep learning model; various common surface and subsurface defects in production are covered; the real-time detection requirement of an industrial production line is met through relative movement of the part and the light source and high-speed image acquisition and processing; the system has strong anti-interference capability, and can adapt to detection scenes of different materials, sizes and environmental conditions.

The invention provides a comprehensive evaluation method and system for the remediation effect of heavy metal contaminated soil, and relates to the technical field of environmental engineering.The method specifically comprises the steps that remote sensing data of a contaminated area is obtained, a soil sample is collected, the microbial respiration rate and the soil physicochemical property of the soil sample are measured, the remote sensing data are processed, and a normalized vegetation index is obtained; generating a soil health assessment index based on the collected physical and chemical properties of the soil; integrating the soil evaluation index, the microbial respiration rate and the normalized vegetation index to construct a soil remediation evaluation index; and comparing the remediation evaluation index with a preset evaluation threshold value, and evaluating the remediation effect of the heavy metal contaminated soil. According to the method, the soil health evaluation index and the remediation evaluation index are constructed, so that the evaluation accuracy of the remediation effect of the heavy metal contaminated soil is effectively improved. Through multivariate correlation analysis, biological, chemical and physical characteristics of the soil can be comprehensively reflected, and a scientific basis is provided for subsequent management and remediation measures.

The invention provides a rock mass real structural surface direct shear value test simulation method, and belongs to the technical field of rock mechanics. Preparing an artificial fracture on the standard rock sample through Brazilian splitting; scanning the surface of the artificial fracture by adopting a three-dimensional scanning technology to obtain point cloud data of a real structural surface of the rock mass; establishing a direct shear test geometric numerical model of the real rock mass structural surface based on the point cloud data; according to the geometric numerical model of the direct shear test of the real rock mass structural plane, establishing a control equation set considering the wear of the real rock mass structural plane; according to the control equation set, numerical simulation of the direct shear test of the real rock mass structural plane considering abrasion is achieved. The method disclosed by the invention lays a solid foundation for accurately knowing and scientifically mastering the shearing mechanical response and wear evolution law of the real structural surface of the rock mass, and improves the accuracy.

The invention belongs to the related technical field of microbiological treatment of soil, and discloses a method for improving microbiological sand consolidation strength based on modified plant fibers. Natural plant fibers are subjected to alkali treatment modification, so that non-cellulose components on the surfaces of the natural plant fibers are removed, the roughness and the specific surface area are remarkably improved, the interface friction force and the mechanical connection capacity between the fibers and sand particles are enhanced, more nucleation sites are provided for microorganisms to induce calcium carbonate precipitation, and the content of calcium carbonate is increased. The strength of the solidified sandy soil can be effectively improved, and the biomineralization period is greatly shortened. Meanwhile, after the modified fibers are doped into the sandy soil, a three-dimensional interlaced fiber network structure can be formed in the material in combination with a microorganism induced calcium carbonate deposition (MICP) technology, and the cohesiveness among sand grains and the mechanical property of the overall structure are effectively improved. According to the solidification method, the solidification strength of the sand column is remarkably improved, the technology is simple, green and environment-friendly, and good engineering adaptability and popularization value are achieved in the field of sand solidification.

The invention discloses a method for determining the lower limit of plugging thickness of a silt rock stratum based on temperature-stress field simulation, which comprises the following steps: judging that the fracture pressure of a shale reservoir is less than the minimum starting pressure of an overlying silt rock stratum, and carrying out the following steps: wrapping the side surface of a roof rock sample with a heat insulation material; arranging heating and stress devices and temperature and strain sensors on the bottom surface of the rock sample, and arranging a plurality of strain sensors on the side surface of the rock sample at set intervals; heating the rock sample by using a heating device until the bottom temperature is the set temperature, applying stress to the bottom of the rock sample from small to large step by step by using a stress device until the set stress, stabilizing the temperature and the stress until the measured values of the sensors are stable, and determining the lowest position where the strain of the rock sample is not higher than the set strain according to the measured values of the sensors; determining the lower limit of top plate plugging thickness according to the distance from the position to the bottom surface; and similarly, determining the bottom plate plugging thickness lower limit. The method can reasonably quantify the lower limit of the plugging thickness of the top and bottom plates of the shale reservoir through joint simulation of the temperature field and the stress field.

Provided are ex vivo systems and methods of predicting the response of a drug or other agent on a tissue. In some embodiments, the systems and methods comprise cutting a tissue into tissue fragments, adding a drug or other agent to the tissue fragments based on an estimated tumor content, and performing an ex vivo measurement on the tissue fragments.

An integrated cleaning and inspection machine, comprising a portal frame (30), wiping mechanisms (40), rotary conveying assemblies (50), and visual inspection mechanisms (60). A plurality of wiping mechanisms (40) are mounted side by side on the front side of the portal frame (30) by means of a first movement mechanism; a plurality of visual inspection mechanisms (60) are mounted side by side on the rear side of the portal frame (30) by means of a second movement mechanism; and two sets of rotary conveying assemblies (50) are respectively provided with a plurality of product jigs (80) capable of swinging and rotating, wherein one set of rotary conveying assembly (50) corresponds to the wiping mechanisms (40), the other set of rotary conveying assembly (50) corresponds to the visual inspection mechanisms (60), and the two sets of rotary conveying assemblies (50) are configured to transfer products when moving to the middle position of the wiping mechanisms (40) and the visual inspection mechanisms (60). The device can achieve multi-angle and multi-surface wiping cleaning and inspection, the occupied space is reduced, and the costs are reduced.

In various embodiments, the disclosure provides compositions, such as polypeptides, polynucleotides, gene editing systems, small molecules, vectors, or host cells, comprising and / or modulating the expression or activity of a cancer-associated protein. In various embodiments, the disclosure also provides methods of treating cancer using agents comprising and / or modulating the expression or activity of a cancer-associated protein, as well as methods of identifying such agents.

The invention relates to a full-automatic QuECHERS experimental device, and relates to the technical field of sample pretreatment, the full-automatic QuECHERS experimental device comprises an extraction unit and a pipetting unit, the extraction unit comprises: a first tube rack module comprising a plurality of test tube racks; a carrying mechanical arm; the first cap screwing module comprises a tube cap clamping and lifting assembly acting on a tube cap, a tube body clamping and rotating assembly acting on a tube body and a first sliding rail assembly for conveying a test tube to a next station; the sample adding module comprises a homogeneous proton adding module and a salt pouring module; the second cap screwing module comprises a pipe body clamping and rotating assembly, a pipe cap clamping and lifting assembly and a second sliding rail assembly for realizing communication and conveying of the extraction unit and the pipetting unit; the invention discloses a pipetting mechanical arm and a gun head frame. The device is provided with the extraction unit capable of synchronously performing extraction operation and the pipetting unit capable of performing pipetting operation, so that the extraction operation and the pipetting operation are synchronously performed during pesticide residue inspection, and the experimental efficiency and the applicability of the device are effectively improved.

The invention discloses a non-targeted screening method for characteristic mass spectrum fingerprints of pollutants in water. The non-targeted screening method comprises the following preparation steps: S1, collecting and filtering; s2, performing mass spectrometry; s3, data processing; s4, constructing a fingerprint spectrum; and S5, performing non-targeted screening. In a characteristic mass spectrum fingerprint construction process, a plurality of advanced data analysis algorithms, principal component analysis, clustering analysis and a chemometrics method are combined, and a real representative characteristic peak can be more effectively mined from massive mass spectrum data in a multi-algorithm fusion mode; meanwhile, various means such as solid-phase extraction and acid-base adjustment are combined, targeted enrichment and treatment are carried out according to pollutant properties, a comprehensive database containing known pollutants and characteristic fingerprints newly found through the method is established, and a perfect index and classification system is configured.

The invention discloses a boiler inner wall crack detection method based on a laser ultrasonic wave and infrared thermal imaging technology. The method comprises the following steps: checking detection equipment; cleaning the inner wall of the boiler; performing preliminary visual inspection on the inner wall of the boiler; laser ultrasonic detection equipment is arranged on the inner wall of the boiler; systematically performing laser ultrasonic scanning on the inner wall of the boiler; an infrared thermal imaging camera is used for scanning the inner wall of the boiler; performing infrared thermal imaging scanning; comparing and analyzing the data; and compiling a detailed detection report. According to the invention, the laser ultrasonic detection technology can detect deep micro cracks on the inner wall of the boiler with high precision, even the micro cracks can be found before the micro cracks become significant problems, and the infrared thermal imaging technology can provide visual information about crack positions and sizes by capturing thermogram differences of crack areas, so that the accuracy of the thermal imaging is improved. This technique is particularly suited for the discovery of surface cracks and for the assessment of the scope of crack impact.

The invention discloses a shaving board detection device, and relates to the technical field of shaving board production, the shaving board detection device comprises a main support, two sets of auxiliary supports and a surface cleaning mechanism, the inner side of the main support is provided with an automatic overturning conveying mechanism, and the inner sides of the two sets of auxiliary supports are fixedly connected with fixing plates; through the arrangement of the automatic turnover conveying mechanism, after the shaving board is clamped by the two clamping plates, when a conveying motor is started to drive a driving roller to rotate, the shaving board can be automatically turned over, and the shaving board can be automatically turned over; the shaving board is conveyed and detected to the position below the detection camera through cooperation of two sets of conveying belts and driven rollers, and the shaving board is turned over by 180 degrees through cooperation of a driven gear, a long toothed bar and other assemblies and then passes through the position below the detection camera to be detected again. Therefore, the effect of accurately detecting the front and back surfaces of the shaving board is achieved.

The invention discloses an alkali metal liquid drop shaping mechanism and an alkali metal content measuring device and method. The alkali metal liquid drop shaping mechanism comprises a shell, an alkali metal liquid drop quasi-circular shaping system, an alkali metal content analysis system and a control system, wherein the alkali metal liquid drop quasi-circular shaping system is used for driving disorderly distributed alkali metal liquid drops to a certain fixed surface of the air chamber and shaping the shape of the alkali metal liquid drops into a quasi-circular shape; the alkali metal content analysis system comprises a gas chamber analysis chamber, a gas chamber placement block, a digital microscope, an annular light source and a development board, and is used for acquiring a shaped alkali metal liquid drop image and processing the image to obtain required information; and the heating assembly, the refrigerating assembly and the alkali metal content analysis system are respectively connected with the control system in a wired or wireless manner. Quasi-circular shaping and content measurement of the alkali metal liquid drops in the air chamber can be realized, so that a more convenient and lossless air chamber internal alkali metal content testing mode is provided for researchers, and service life information of the alkali metal air chamber is perfected.