1449results about "Animal reproduction" patented technology

The invention provides a method of cloning a reproductive and respiratory syndrome resisting pig. The method comprises the following steps: transferring CRISPR / Cas9 targeting vectors and CD163 gene homologous recombination modification vectors into fibroblasts of a pig to obtain positive clone cells, wherein the seventh exon of the endogenous CD163 gene in the positive clone cells of the pig is replaced by the tenth exon of the CD163-L1 gene of human, so that the positive clone cells are incapable of mediating PRRSV invading; obtaining a clone embryo by adopting a somatic cell nucleus transfer technology by taking positive cells as nucleus transfer donor cells and oocytes as nucleus transfer recipient cells; and transferring the clone embryo into the uterus of the pig, and impregnating to obtain a cloned pig. The method provided by the invention is low in cost, the time of obtaining the homozygous pig is shortened greatly, the expression of the CD163 gene is not affected by gene editing, and a foundation is laid for the research into the gene function of large animals and the establishment of disease models on the basis of CRISPR / Cas9 mediated homologous recombination.

A monitoring system (800) is operative for receiving sensor signals and analyzing the sensor signals to identify events or conditions of interest, such as colic, with respect to a monitored animal. The sensors may be animal-borne sensors such as motion related sensors. The system (800) includes a processor (802) operative to identify the conditions of interest based on a stored pattern database (806), threshold tables (808) and heuristic engines (812). Upon identification of a condition of interest, a human expert may be consulted and alerts may be generated as required.