18 results about "Estrone" patented technology

Provided are methods for determining the amount of estrone in a sample using mass spectrometry. The methods generally involve ionizing estrone in a sample and detecting and quantifying the amount of the ion to determine the amount of estrone in the sample.

The invention relates to the technical field of dairy product detection, in particular to a method for simultaneously detecting three steroid hormone substances in raw milk. The three steroid hormone substances are estradiol, ethinyl estradiol and estrone respectively, and the detection method comprises the following steps: 1) removing protein in raw milk to obtain a sample solution to be detected; (2) carrying out qualitative and quantitative analysis on the three steroid hormone substances in the sample solution to be detected by adopting a high performance liquid chromatography-ultraviolet detection method; wherein the qualitative analysis is based on the retention time of each substance, and the quantitative analysis is based on a standard curvilinear equation to calculate the concentration. According to the detection method, the dependence on expensive instruments and professional operators is remarkably reduced while high sensitivity and accuracy are guaranteed, the cost is low, and the method is particularly suitable for rapid screening of grassroots units and large-batch samples.

The invention discloses a positive ion metabolism marker for thyroid-associated ophthalmopathy, a product and application of the positive ion metabolism marker. The positive ion marker is prepared from any one or more of L-arginine-L-valeryl-L-lysine, cholesterol beta-epoxide, phosphatidylcholine (18: 4 / 20: 5), sphingosine galactoside and oestrone. The invention provides a kit which comprises a detection reagent for detecting the positive ion marker. A computer program product associated with thyroid-related ocular diseases is provided. The product provided by the invention has good feasibility and accuracy, can effectively evaluate the risk of thyroid-related eye diseases, and provides a new tool for clinical diagnosis.

To provide a method capable of conveniently and accurately determining stress.SOLUTION: The present invention provides a method for acquiring stress determination data, the method including measuring a stress determination marker that includes at least one selected from pyruvic acid, nicotinamide, Ile, N-acetyl-β-alanine, 2AB, choline, Val, uracil, xanthine, estrone, inosine, adenosine, and Leu in a biological sample derived from a subject, or includes a combination of at least one selected from propionic acid, spermine, 3-methylbutanoic acid, 4-methyl-2-oxopentanoic acid, diethanolamine, putrescine, and body temperature together with at least one of the above.SELECTED DRAWING: Figure 1

The invention belongs to the technical field of immunodetection, and particularly relates to a specific binding antibody of estradiol as well as a preparation method and application of the specific binding antibody. The present application provides a specific binding antibody against estradiol (E2). The binding protein adopts rational design guided by computational simulation, the complementary determining regions (CDRs) of the binding protein are accurately optimized, the binding protein can target an estradiol characteristic structural domain with high affinity, structural analogues such as oestrone, estriol and corticoid can be effectively distinguished, and the binding protein shows excellent specificity. When being applied to the development of an in-vitro diagnostic kit, the aptamer can realize high-sensitivity and high-specificity quantitative detection of estradiol in a clinical serum sample, and a reliable tool is provided for precise diagnosis and treatment of related diseases.

This invention relates to the field of water treatment technology and discloses an intermittent electrocatalytic treatment method for trace estrogen in reclaimed water. The method is carried out in a three-dimensional electrode reactor. Before formal treatment, a pre-treated water sample containing the estrogen to be removed is used to pre-saturate the particle electrode. Subsequently, reclaimed water containing one or more estrogenic pollutants is continuously introduced into the reactor, operating at a current density of 1.0–10.0 mA / cm², a hydraulic retention time of 5–20 min, and an aeration rate of 0–3.0 L / min. An intermittent power supply mode with alternating power on and off is employed, wherein the ratio of power on time to power off time is 1:1, and the power off time is 2–120 s. During the power off phase, the estrogen to be removed accumulates on the surface of the particle electrode. During the power on phase, the particle electrode repolarizes to form a micro-battery and undergoes electrochemical oxidation in conjunction with the anode, causing direct oxidation and / or indirect oxidation mediated by an electrogenerated oxidant, while the particle electrode achieves in-situ regeneration. The method is applicable to the treatment of trace amounts of estrogens in reclaimed water, preferably one or more of estrone (E1), 17β-estradiol (E2), estriol (E3), 17α-ethinylestradiol (EE2), and bisphenol A (BPA). This invention can reduce the artificially inflated impact of adsorption on the evaluation of treatment effectiveness, and reduce energy consumption while ensuring treatment efficiency.

The invention belongs to the technical field of chemical synthesis, and particularly relates to an asymmetric synthesis method of an alpha-chiral ester compound. Comprising the following step: in a solvent, performing asymmetric alpha-protonation and esterification reaction on alpha-alkyl-alpha, beta-unsaturated aldehyde and alcohol as shown in a formula II in the presence of a chiral N-heterocyclic carbene catalyst, alkali and protonic acid to obtain the alpha-chiral ester compound. The invention develops a redox esterification reaction based on chiral N-heterocyclic carbene catalysis, so that the redox esterification reaction can be efficiently suitable for various alpha-alkyl alpha, beta-olefine aldehydes from simple to high steric hindrance and from chain to polycyclic; the catalytic strategy can be directly applied to later directional esterification modification of important natural product derivatives such as oestrone and epiandrosterone, and a powerful tool is provided for research and development of new drugs.

The invention relates to the field of pharmaceutical analysis methods, in particular to a method for determining the content of potential related substances in estrone prepared through a synthesis process. The method disclosed by the invention can be used for simultaneously determining the estrone and seven related substances thereof in the estrone prepared by a chemical synthesis process. The method has strong specificity and high sensitivity, and is suitable for estrone synthesis process optimization, intermediate product quality control and finished product quality detection.

The invention provides a method for biologically preparing estrone by using 3-sterone-delta-dehydrogenase, which comprises the following steps: taking 19-hydroxy-4-androstene-3, 17-diketone as a substrate, and reacting with the 3-sterone-delta-dehydrogenase KstD to obtain the estrone; wherein the amino acid sequence of the 3-ketosteroid-delta-dehydrogenase KstD is as shown in SEQ ID NO. 1. The whole synthesis process is simple, few in steps, short in period, few in side reaction, mild in reaction condition, low in equipment requirement, free of pollution to the environment and environmentally friendly.

The invention discloses a synthesis method of estradiol semihydrate. The synthesis method is particularly suitable for a process of synthesizing the estradiol semihydrate by taking ADD (1.4-androstenedione) as an initial raw material. The method comprises the following steps: firstly, carrying out ketal reaction on ADD and ethylene glycol to generate ketal, reacting the ketal with a lithiation reagent prepared from biphenyl, diphenylmethane, lithium and tetrahydrofuran to generate 17-ketal estrone, then hydrolyzing the 17-ketal estrone through inorganic acid to obtain estrone, reducing the estrone through a reducing agent to obtain an estradiol crude product, and purifying the estradiol crude product. And refining the estradiol crude product with an alcohol or ketone solvent and pure water, and drying to obtain the estradiol semihydrate. By using the method, impurities such as biphenyl, diphenylmethane and the like in lithiation reaction are removed in advance, the refining operation of the estrone is reduced (the refining of the estrone requires 30 times of volume of a solvent), the productivity is improved, the refining and drying processes of the estradiol semihydrate ensure that the content of crystal water is qualified, the single impurity of the synthesized product is less than 0.1%, and the yield is improved. The product meets the quality standards of the Chinese pharmacopoeia 2020 edition and the European pharmacopoeia 11 edition.

The present invention relates to a method for determining the presence of lung cancer in a subject. The method comprises: (i) determining the level of one or more biomarkers in a sample from the subject; and (ii) comparing the level of said one or more biomarkers with the level of said one or more metabolites in a control sample to determine whether lung cancer is present in the subject. The biomarkers are selected from: 2-methoxyestrone 3-sulfate, testosterone glucuronide, androsterone glucuronide, 5a-dihydrotestosterone sulfate, corticrocin, octanoic acid, beta-citryl-L-glutamic acid, 2-hydroxymuconic semialdehyde, 5,6,11-dodecatriynoic acid, creatine riboside, 3-(Carboxymethyl)-3-hydroxypentanedioic acid, adenosine phosphosulfate, 7,8-dihydroneopterin 3'-phosphate, cis,cis,cis-10,13,16-Docosatrienoyl-CoA, inosine, N-(2,4-Dinitrophenyl)-2,4-dinitroaniline, prostaglandin M, pentadecenoic acid, PE(22:0 / P-18:0), carbamoyl phosphate, 2-hydroxy-3-oxohexanedioic acid, galactosylceramide (d18:1 / 22:0) and PE-NMe2(18:1(11Z) / 22:1(13Z)).

The application discloses a wheat Tilletia indica-specific biomarker combination, a screening method and application thereof, and the biomarker combination comprises 20-hydroxyarachidonic acid, 1-octadecyl lysophosphatidic acid, linolenic acid, 13-hydroxydocosahexaenoic acid, 4-methoxy estrone, hypoxanthine, 3-hydroxymyristic acid, beta-ecdysterone, 17-phenyltrinorprostaglandin F2 alpha cyclopropylmethylamide, 11,12-dihydroxyeicosatrienoic acid and oxidized octadecadienoic acid. When the biomarker combination is used for identifying Tilletia indica, the time-consuming long and the accuracy easily interfered by the spore development stage limitations of traditional morphological identification are overcome; in view of the molecular level identification difficulty caused by the high genome homology of Tilletia indica and Tilletia controversa, the application digs the feature difference at the level of metabolomics, and constructs a specific chemical fingerprint, so that the technical bottleneck that the close relatives are difficult to accurately distinguish is effectively solved.

This invention discloses a method for detecting steroid hormones using furoyl chloride derivatization-liquid chromatography-tandem mass spectrometry. This invention utilizes furoyl chloride to derivatize steroid hormones, and the combination with liquid chromatography-tandem mass spectrometry enables highly sensitive and accurate quantification of trace amounts of steroid hormones such as estrone and estradiol in biological matrices. Compared with existing technologies, the method for detecting steroid hormones using furoyl chloride derivatization-liquid chromatography-tandem mass spectrometry provided by this invention exhibits high recovery, a wide linear range, good linearity, low detection limit, and mild reaction conditions with a simplified reaction procedure.

The application discloses a test paper for detecting estrone and a preparation method thereof, which comprises the following steps: (1) preparing a CsYF4@Yb2O3 compound, adsorbing estrone antibodies, i.e. first antibodies, on the surface of the compound to obtain a photothermal signal probe; (2) assembling a test paper strip, fixing estrone antigens and second antibodies on a detection area and a quality control area respectively; (3) mixing the photothermal signal probe with a sample liquid to be detected, and then inserting a sample pad end of the test paper strip into the mixed liquid to perform chromatography; and (4) detecting a photothermal signal: irradiating the detection area with a near-infrared LED, collecting temperature by using a thermal imaging or temperature measuring device, and performing quantitative analysis according to a linear relationship between the temperature of the detection area and the estrone concentration of the sample. The detection mode is flexible, has a wide linear range, high sensitivity and good specificity.

The invention discloses a preparation method of 17-(4 '-phenyl phenyl)-estra-1, 3, 5 (10)-triene-3, 17 beta-diol and an application of the 17-(4'-phenyl phenyl)-estra-1, 3, 5 (10)-triene-3, 17 beta-diol. The method comprises the following steps: by taking estrone as an initial raw material, carrying out hydroxyl protection, carrying out lithium-halogen exchange on n-butyllithium and 4-bromodiphenyl to generate a high-activity biphenyl lithium intermediate, then carrying out nucleophilic addition on the high-activity biphenyl lithium intermediate and a protected estrone derivative, and finally, carrying out hydrolysis deprotection to obtain a target product. According to the method, a traditional and low-efficiency Grignard reaction route is successfully replaced with a lithium-halogen exchange strategy, the technical bottlenecks that the steric hindrance of a substrate is large and the reaction is difficult to carry out are overcome, the method has the advantages of being easy and convenient to operate, mild in reaction condition, high in product yield and good in purity, and a reliable scheme is provided for preparing the key impurity standard substance.

The invention belongs to the technical field of steroid drug molecules, and relates to an estrone purification method, which comprises the following steps: adding 1, 4-diazabicyclo [2.2. 2] octane into a solution containing an estrone crude product and a first solvent to obtain a precipitate, dissolving the precipitate in a second solvent, adjusting to be alkaline, filtering, collecting filtrate, adjusting the pH value of the filtrate to be less than or equal to 3, and filtering to obtain the estrone purification product. And extracting with a third solvent to obtain an organic phase, cooling, crystallizing, carrying out suction filtration, and drying to obtain refined estrone. According to the method, the problem of removal of methylation rearrangement impurities generated in the existing preparation technology of the estrone is solved, the methylation rearrangement impurities can be purified from 0.7%-1.5% to less than or equal to 0.10% at a time, the purity of the finally obtained estrone is greater than or equal to 99.8%, and the yield is stable. The method disclosed by the invention is green and environment-friendly, low in cost, less in material loss, good in purification effect and stable in process, and has extremely high industrial application value.

The invention discloses an oestrone antibody and application thereof, and relates to the field of antibodies. The oestrone antibody disclosed by the invention comprises a heavy chain complementarity determining region and a light chain complementarity determining region, provides an important raw material source for oestrone detection, and has good affinity or activity.

A method for determining the presence of lung cancer in a subject comprises: (i) determining the level of one or more biomarkers in a sample from the subject; and (ii) comparing the level of said one or more biomarkers with the level of said one or more metabolites in a control sample to determine whether lung cancer is present in the subject. The biomarkers are selected from: 2-methoxyestrone 3-sulfate, testosterone glucuronide, androsterone glucuronide, 5a-dihydrotestosterone sulfate, corticrocin, octanoic acid, beta-citryl-L-glutamic acid, 2-hydroxymuconic semialdehyde, 5,6,11-dodecatriynoic acid, creatine riboside, 3-(carboxymethyl)-3-hydroxypentanedioic acid, adenosine phosphosulfate, 7,8-dihydroneopterin 3’-phosphate, cis,cis,cis-10,13,16-docosatrienoyl-CoA, inosine, N- (2,4-dinitrophenyl)-2,4-dinitroaniline, prostaglandin M, pentadecenoic acid, PE(22:0 / P-18:0), carbamoyl phosphate, 2-hydroxy-3-oxohexanedioic acid, galactosylceramide (d18:1 / 22:0) and PE-NMe2(18:1(11Z) / 22:1(13Z)). The biomarkers may be detected using mass spectrometry, or by enzyme-linked immunosorbent assay (ELISA). Preferably, the sample is a urine sample.