55 results about "Ecdysterone" patented technology

The invention discloses a method for extracting ecdysterone from a plant, which is characterized by comprising the following steps of: a, grinding a raw material, adding biological enzyme, performing enzymolysis at the temperature of between 40 and 50 DEG C for 2 to 3 hours, adding water, raising the temperature to be between 70 and 80 DEG C, preserving heat, soaking for 1 to 2 hours, extracting for two to three times, and filtering to obtain extract; and b, adding an ultrafiltration membrane into the extract for ultrafiltration, adding a nanofiltration membrane into permeate for concentrating, adding ethanol solution with the same volume as concentrated solution into the concentrated solution for filtering, adding ethyl acetate for extracting, adding activated carbon into raffinate for reflux decolorization, filtering and concentrating decolorized solution until ethanol is not crystallized, and drying crystals to obtain the ecdysterone. The method is easy to operate, high in extraction efficiency, low in energy consumption and suitable for industrial production.

The invention discloses feed capable of promoting molt of crayfish and a preparation method thereof and belongs to the field of agricultural feed. The feed is prepared by mixing rice bran powder, soybean meal, fish meal, seaweed powder, bone meal, squid liver paste, cholesterol powder, tricalcium phosphate, calcium hydrogen phosphate, vitamin D3, tea seed meal, potassium dihydrogen phosphate, bile acid and aquatic complex microbial agent and then performing fermentation. The feed has the advantages that the molting hormone of the crayfish is increased by promoting the crayfish to absorb the high cholesterol in the feed to synthesize ecdysterone so as to increase the molt and growth speed of the crayfish; the feed can promote the molt of the crayfish, increase the immunity of the crayfish, increase the number of intestinal tract beneficial bacteria and purify water; drugs such as hormone and antibiotics are not added into the feed, and the feed is simple to prepare, safe in raw materials, rich in nutrition, scientific in compatibility, good in feeding attraction performance, low in cost and capable of increasing benefits.

The invention discloses a quality control method of a traditional Chinese medicine compound preparation for treating osteoporosis. The quality control method comprises the following steps: (1) by taking eucommia ulmoides as a contrast medicinal material and taking pinoresinol diglucoside and geniposidic acid as comparison products, carrying out qualitative identification on eucommia ulmoides in the traditional Chinese medicine compound preparation by adopting a thin-layer chromatography; (2) by taking radix achyranthis bidentatae as a contrast medicinal material and taking beta-ecdysterone as a comparison product, carrying out qualitative identification on radix achyranthis bidentatae in the traditional Chinese medicine compound preparation by adopting the thin-layer chromatography; (3) by taking wolfberry fruits as contrast medicinal materials, carrying out qualitative identification on the wolfberry fruits in the traditional Chinese medicine compound preparation by adopting the thin-layer chromatography; and (4) determining the content of three active components (or index components) including epimedium glucoside, gentiopicroside and loganin acid in the traditional Chinese medicine compound preparation by adopting a high performance liquid chromatography. According to the quality control method, the quality of the traditional Chinese medicine compound preparation can be controlled very well, and the stability of a product production process is monitored; and the quality of the traditional Chinese medicine compound preparation is stable, uniform and controllable.

The invention discloses feed capable of promoting the molt of Penaeus vannamei and a preparation method thereof and belongs to the field of agricultural feed. The feed is prepared by mixing rice bran, bran, bean cake, minced fish meat, seaweed meal, squid liver paste, shrimp shell meal, salted duck egg, bile acid, radix astragali, Eucommia ulmoides, Adenophora stricta, radix rehmanniae preparata, dendrobium, fructus schizandrae, ginseng leaf, trace element agent and microbial fermentation agent and then performing fermentation. The feed has the advantages that the molting hormone of the Penaeus vannamei is increased by promoting the Penaeus vannamei to absorb the high cholesterol in the feed to synthesize ecdysterone so as to increase the molt and growth speed of the Penaeus vannamei; the feed can promote the molt of the Penaeus vannamei, increase the immunity of the Penaeus vannamei, increase the number of intestinal tract beneficial bacteria and purify water; drugs such as hormone and antibiotics are not added into the feed, and the feed is simple to prepare, safe in component, rich in nutrition, scientific in compatibility, good in feeding attraction performance and low in cost.

The invention discloses a beta-ecdysterone-rich Cyanotis arachnoids extraction and purification method. The content of beta-ecdysterone in an extract product is 50-100%, and the extract product is prepared from Cyanotis arachnoids through the steps of water refluxing extraction, alcohol precipitation, resin adsorption and recrystallization. The content of the beta-ecdysterone in the Cyanotis arachnoids extract product obtained through adopting the extraction method is greatly improved, and the purity of the extract product reaches 90% or above. The above preparation method is suitable for industrial large-scale production, and is cheap.

The invention discloses an application of B-ecdysterone in making medicine for curing and/or preventing osteoporosis. The invention respectively studies the protective action of B-ecdysterone on bone metabolism at both general level and cellular level. When cultured together with osteoblast strain MC3T3-E1 and primary mice marrow stromal stem cell, B-ecdysterone can obviously promote the osteogenic differentiation of both cells. A mice osteoporosis model is established through tretinoin lavage, and the in vivo activity experiment of B-ecdysterone is carried out through adopting an intraperitoneal injection method and a lavage method; the results show that B-ecdysterone has the functions of increasing bone density, the content of mineral matters in bone tissues and thighbone diameter and improving hydroxyproline enzymatic activity. Therefore, B-ecdysterone can be used to make medicine for curing and/or preventing osteoporosis as well as medicine for promoting osteogenic differentiation and bone healing.

The invention discloses a cultivation feed for adult Changjiang crabs. The cultivation feed contains the following components in parts by weight: 1-3 parts of choline chloride, 5-10 parts of plant oil, 50-60 parts of high protein flour, 35-45 parts of bean pulp, 0.5-0.8 part of lactic acid bacteria-produced extracellular polysaccharide, 0.015-0.02 part of sesbania powder, 18-30 parts of corn starch, 0.1-0.3 part of a composite mineral substance additive, 0.03-0.1 part of zinc polycarboxylate, 0.15-0.2 part of ecdysterone, 3-5 parts of table salt and 2-5 parts of calcium hydrogen phosphate. According to the cultivation feed, the nutritive values of mineral elements can be increased; and furthermore, the cultivation feed is capable of releasing Ca<2+> which can participate in cross-linking or other reactions so as to change the textures of plant foods, sufficient nutrients can be supplied to adult crabs, the production can be promoted, and the nutritional value of the crabs is high.

The invention provides a method for detecting content of ecdysterone in an ecdysterone containing medicinal composition. The method is characterized in that a chromatographic column is an octadecyl silicane bonded silica gel packed column; an ultraviolet visible light detector is adopted for detecting an analysis result; the detection wave length is between 240 and 255nm; a mobile phase can be acetonitrile-water; and the column temperature is between 25 and 35 DEG C. The invention also provides a method for controlling the product quality by tracking the concentration of the ecdysterone in extracting solution through high performance liquid chromatography in a decoction or extraction link of medicinal materials, wherein the decoction or extraction operation is stopped when a target value is reached. The method has the advantages of simple operation, strong specificity and good repeatability, and high accuracy of a content detection result, and can better control the product quality.

The invention discloses a scorpion feed. The scorpion feed comprises the following components in parts by weight: 10-20 parts of milk powder, 8-15 parts of toad meat meal, 3-5 parts of biscuits, 5-8 parts of bran, 3-8 parts of calcium phosphate, 5-10 parts of ecdysterone, 5-10 parts of preservatives, 5-10 parts of vitamins, 5-8 parts of essence and 10-20 parts of distilled water. A preparation method of the scorpion feed comprises the following steps: (1) adding the biscuits and the bran into a grinder according to the proportion, carrying out stirring grinding, enabling the ground mixtures to pass through an 80-mesh sieve, adding the toad meat meal, the calcium phosphate, the ecdysterone, the preservatives, the vitamins and the essence according to the proportion, and uniformly stirring for further use; (2) dissolving the milk powder into the distilled water according to the proportion for further use; and (3) after mixing standby materials of the step (1) and the step (2), carrying out drying, granulation, sterilization and vacuum packaging so as to obtain the scorpion feed. The scorpion feed is low in cost and can be adapted to the growth law of scorpions; according to the scorpion feed, a wide prospect is provided for the efficient and artificial breeding of the scorpion.

The invention discloses a preparation method of feed capable of promoting hairy crabs to change shells. The preparation method comprises the following steps: extraction of fish viscera oil, modification of fish oil, fermentation of fishmeal, fermentation of cottonseed meal and compounding. The feed capable of promoting the hairy crabs to change shells is composed of the following materials in parts by weight: 20-25 parts of modified fish oil, 15-20 parts of fermented fishmeal, 40-50 parts of fermented bean meal, 10-12 parts of flour, 2-5 parts of table salt, 0.2-0.3 part of ecdysterone, 0.2-0.3 part of squid cream, 3-5 parts of shrimp meal powder, 0.5-1.0 part of carboxymethyl cellulose, 3-5 parts of compound premix, 0.005-0.006 part of astaxanthin and 0.005-0.006 part of canthaxanthin. The feed capable of promoting the hairy crabs to change shells has the beneficial effects: the feed has stronger oxidation resistance, can clear away excessive free radicals from the bodies of the hairycrabs, improves the immunity and anti-stress ability of the hairy crabs, improves the constitutions and vitality of the hairy crabs in a shell-changing period and enables the hairy crabs to get through the shell-changing period safely.

The invention discloses a feed formula for fishes, shrimps and crabs. According to the formula, a feed is prepared from, by weight, 25-40 parts of fish meal, 20-25 parts of bean pulp, 10 parts of peanut meal, 5 parts of shrimp shell powder, 1 part of shell powder, 3 parts of cottonseed meal, 2 parts of meat and bone meal, 18-20 parts of dry flour, 2-5 parts of yeast powder, 1 part of fish oil, 3-4parts of husk bran, 1 part of a premix, 0.1 part of choline, 1-2 parts of monopotassium phosphate, 0-0.2 part of seaweed meal, 0.1 part of ecdysterone, 0.05 part of garlic, 0.2 part of bacillus lactate, 0.3 part of a binder and 0.2 part of salt. According to the formula, the garlic is utilized for helping digestion and immunization of the intestinal tracts of the fishes, shrimps and crabs while calcium is supplemented, the survival rate is increased, the growth is accelerated, and the economic benefit is guaranteed.

The invention relates to anti-liver-cancer strawberry beer and a production method thereof. Strawberries are squeezed into juice to be mixed with mulberry juice and malt juice and protease is added for enzymatic hydrolysis to obtain an enzymatic hydrolysis solution; spinach roots and Chinese sage herbs are crushed into 30 meshes, to be soaked in the 95% of ethanol for 2 to 3 hours and the ethanol is recycled to obtain a leaching solution; the enzymatic hydrolysis solution is mixed with the leaching solution, beer yeast is added, the fermentation is performed under 32 to 34 DEG C, and the anti-liver-cancer strawberry beer is obtained after filtration. According to the anti-liver-cancer health care strawberry beer, nutrients are rich and accordingly the intrahepatic inflammation in a human body can be eliminated and the roof of the production of a liver cancer is blocked from the essence; abundant anthocyanins are rich and accordingly more than one third of cancer cells in the body can be killed; anti-liver-cancer components such as ecdysterone, tannin and sesquiterpenoids are contained in the strawberry beer which is produced after the fermentation and accordingly the protease can be activated to induce tumor cell apoptosis and accordingly the processes such as metabolism, proliferation, differentiation, signal transmission and gene expression of tumor cells are affected and the growth of the tumor cells can be blocked.

The invention discloses a serum-free adipose-derived stem cell culture medium and a culture method of adipose-derived stem cells (ADSCs). The culture medium comprises a basic culture medium body and additives, the basic culture base body is a DMEM (dulbecco's modified eagle medium)/F12, the additives include transferrin, fibroblasts growth factors, C-algocyan, acanthopanax senticosus saponins B, scabiolide-3-O-glucosides and beta-ecdysterone. By the arrangement, the growth and proliferation of the ADSCs can be promoted, the damage of proliferation activity of the ADSCs during culture can be reduced, and thus, the in-vitro amplification efficiency of the ADSCs is greatly improved.

The invention discloses a cut rose flower preservative and a method thereof for preserving cut rose flowers. The invention relates to flower treatment. The invention aims at providing the cut rose flower preservative with good preservation effect, low cost, no toxicity and environment-friendly property and the method thereof for preserving the cut rose flowers. The technical scheme of the invention is that the cut rose flower preservative adopts ecdysterone. The method for preserving the cut rose flowers comprises the steps of diluting the ecdysterone with tap water or distilled water to the concentration of 1.6*10<-7>-4.8*10<-7>, placing the collected cut rose flowers into the diluted ecdysterone water solution, carrying out pre-treatment for 24 hours under the condition of 4-9 DEG C, then inserting the cut rose flowers into a bottle containing the tap water or the distilled water, and changing the water once a day. The method for preserving the cut rose flowers comprises the steps of firstly diluting the ecdysterone with the tap water or the distilled water to 10<-10>-10<-6> mol/L, then arranging into the bottle, inserting the cut rose flowers collected at the appropriate time into the bottle, and changing the ecdysterone water solution once a day. The invention is used for preserving the cut flowers.

The invention provides a neonicotinoid insecticide, comprising the following components in parts by mass: 15-30 parts of thiamethoxam; 10-20 parts of cyclopyrid; 8-12 parts of diflubenzuron; ecdysone 0.1-2 parts; emulsifier 1-3 parts; disintegrant 0.3-0.9 parts; dispersant 0.8-2 parts; antifreeze agent 0.2-0.8 parts; carrier 60-120 parts. The neonicotinoid insecticide of the present invention contains a variety of active ingredients, which can improve the control effect; has a long duration; contains non-single active ingredients, and is not easy to produce drug resistance; can prevent and control a variety of different types of pests that occur in combination, thereby It can reduce the dosage of medicine, save the cost and reduce the pollution to the environment.

The invention discloses a method for regulating and controlling peltate yam rhizome triploid plants to root. The method is characterized by comprising the specific steps of selecting peltate yam rhizome triploid regeneration buds which grow healthily, and cutting the regeneration buds into stems with buds, which are 2-3cm long, on a clean bench; inoculating the stems into a culture medium, wherein the culture medium comprises a 1/2MS, 0.3mg/L of NAA (Naphthalene Acetic Acid), 0.24-0.96mg/L of ecdysterone, 30000mg/L of sucrose and 7500mg/L of agar powder, and the pH value of the culture medium is 5.8-6.0; and culturing under aseptic conditions, wherein the culture conditions are as follows: the temperature is 22-24 DEG C, the stems are cultured at a bright place for 16 hours and at a dark place for 8 hours, the relative humidity is 80%-90% and the illumination intensity is 2000-3000Lx. The method has a remarkable facilitation effect on the rooting of the peltate yam rhizome triploid plants; and particularly, the rooting starting time can be shortened, the average root length and the average rooting quantity are remarkably increased, and the transplanting survival rate is also remarkably improved.

The invention provides a method for establishing a UPLC spectrum of radix achyranthis bidentatae and radix achyranthis bidentatae preparata with wine. The method for establishing a UPLC spectrum of radix achyranthis bidentatae and radix achyranthis bidentatae preparata with wine comprises the following steps: preparation of a test solution: taking radix achyranthis bidentatae or radix achyranthisbidentatae preparata with wine test sample powder, adding 10% methanol, carrying out ultrasonic treatment, filtering, and taking filtrate to obtain the test solution; and detecting by adopting a highperformance liquid chromatograph, namely detecting the test solution by adopting a Waters high performance liquid chromatograph, and establishing a UPLC spectrum. Methods for identifying comprise thesteps of detecting whether 5-hydroxymethylfurfural exists in a test solution or not; and if 5-hydroxymethylfurfural is detected, identifying the sample as radix achyranthis bidentatae preparata with wine; alternatively, analyzing whether characteristic peaks appear within the relative retention time ranges of 0.27+/-10%, 0.37+/-10% and 0.41+/-10% or not by taking beta-ecdysterone as a reference peak in the UPLC spectrum, and if one or more characteristic peaks appear, identifying the sample as the radix achyranthis bidentatae preparata with wine.

The invention discloses a preparation method of silkworm ecdysterone. The preparation method comprises the following steps of: boiling, refluxing and extracting decumbent bugle herb; clarifying the collected filtrate by using a clarificant and then performing reduced pressure concentration to obtain a solid residue; dissolving the solid residue by using a methanol-ethyl acetate solution; performing reduced pressure concentration to remove an organic solvent and then obtaining a crude product of an ecdysterone extract; separating and purifying the ecdysterone by using macroporous resin and a silica-gel chromatographic column; and finally performing vacuum concentration to obtain an ecdysterone dry product. The ecdysial fluid prepared from the ecdysterone dry product prepared by the preparation method is high in purity and can be used in a quantified mode, the ecdysterone dry product is convenient to store, the storage time is long, and by adopting the ecdysial fluid prepared from the ecdysterone dry product, domestic silkworms grow and develop neatly and are mounted neatly.

The invention relates to a method for detecting effective components of a traditional Chinese medicine composition for treating kidney diseases, which comprises the step of detecting the effective components of coptis chinensis, salvia miltiorrhiza, pericarpium citri reticulatae and/or achyranthes bidentata, such as berberine hydrochloride, ecdysterone, hesperidin and/or protocatechualdehyde by adopting thin-layer chromatography. The thin-layer chromatography provided by the invention can qualitatively detect the effective components of the traditional Chinese medicine composition for treating kidney diseases, is simple and convenient to operate, good in repeatability and stability and accurate in result, and enhances the rationality and reliability of the whole effective component detection method as a qualitative detection method for the effective components of the traditional Chinese medicine compound preparation.

The invention discloses a compound povidone-iodine solution for a crab shelling period and a preparation method of the compound povidone-iodine solution, and belongs to the technical field of aquaticproduct disinfection. The compound povidone-iodine solution provided by the invention is prepared from the following components in percentage by mass: 10 to 50 percent of a povidone-iodine solution, 1to 20 percent of 20-hydroxyecdysterone, 30 to 45 percent of a solution A and 10 to 35 percent of a surfactant, wherein the solution A is prepared from the following components in percentage by mass:20 to 50 percent of rheum officinale, 10 to 35 percent of cortex phellodendri, 10 to 35 percent of radix scutellariae and 20 to 50 percent of purified water. According to the compound povidone-iodinesolution provided by the invention, the disinfection effect of the povidone-iodine solution is not influenced, and meanwhile, the synchronous shelling rate of crabs can be increased, the immunity of soft-shell crabs in the shelling period of the crabs is improved, autogenous residual food is avoided, the effective iodine content is high, and the stability is better.

The invention discloses a formula of a feed for fishes, shrimps and crabs, wherein each part of the feed comprises 25-30 g of fish powder, 20-23 g of bean meal, 10 g of peanut meal, 5 g of shrimp shell powder, 1 g of shell powder, 3 g of cottonseed meal, 2 g of meat and bone meal, 16-18 g of flour, 2-3 g of yeast powder, 1 g of fish oil, 3-4 g of bran, 1 g of premix, 0.1 g of choline, 0.2 g of natural betaine, 1.8-2 g of potassium dihydrogen phosphate, 0-0.2 g of seaweed powder, 0.1 g of ecdysterone, 0.05 g of garlic, 0.2 g of Lactobacillus sporogenes, 0.2 g of enzyme producing probiotics, 0.3 g of a binder, and 0.2 g of salt. According to the present invention, the calcium is supplemented while the intestinal digestion and the immunity of fishes, shrimps and crabs are helped by using the garlic, the Lactobacillus sporogenes and the enzyme producing probiotics, such that the survival rate is improved, the growth is accelerated, and the economic benefits are ensured.

The invention discloses an ointment for promoting recovery of injured meniscus during plaster immobilization and a preparation method thereof.The ointment is prepared from catechinic acid, thymol crystals, ecdysterone, neottianthe pseudo-diphylax extract, quercetin, hyperoside, diphenhydramine hydrochloride, protopine, bufexamac, kaempferol, aescin, an ointment substrate and an antioxidant.Based on reasonable matching, the various effective ingredients are combined, and the ointment has the effects of promoting blood flow to remove stasis, diminishing inflammation to relieve pain, strengthening the bones and muscles, resisting infection and irritability and preventing skin rotting, is used before plaster immobilization is adopted for non-surgical treatment of injured meniscus and applied to the wound to promote body recovery, can prevent skin infection and irritability symptoms caused by long-time plaster immobilization, and is good in treatment effect, convenient and easy to use and applicable to clinic application.

The invention discloses a detection method of a Chinese herbal compound containing cinnamon, and the detection method comprises the following steps: a Chinese herbal compound test solution and a reference solution are taken for detection, the Chinese herbal compound is composed of angelica sinensis, ligusticum wallichii, radix paeoniae alba, cinnamon, moutan bark, curcuma zedoary, ginseng, liquorice and radix achyranthis bidentatae; the reference substances are gallic acid, paeoniflorin, beta-ecdysterone, ferulic acid, liquiritin, cinnamaldehyde, paeonol, ammonium glycyrrhizinate, ginsenoside Rg1 and ginsenoside Re, and the chromatographic conditions of the detection are as follows: a chromatographic column with phenyl bonded silica gel as a filler is adopted, a mobile phase A is selected from one or more of acetonitrile, methanol and tetrahydrofuran, a mobile phase B is selected from one or more of acetonitrile, methanol and tetrahydrofuran, and a mobile phase C is selected from one or more of acetonitrile, methanol and tetrahydrofuran; the mobile phase B is an acid aqueous solution or a buffer salt aqueous solution, and obtaining component information or component and content information of the traditional Chinese medicine compound according to a detection result. The chemical components of the traditional Chinese medicine compound containing cinnamon are comprehensively and systematically analyzed, and a theoretical basis is provided for deep research on quality control and pharmacodynamic material basis.

The invention discloses a method for extracting and refining ecdysterone. The method comprises the following steps: drying and crushing cyanotis arachnoidea; extracting with ethanol; filtering ; adding a ZTC1 + 1 clarifying agent into the extracting solution, stirring and precipitating, and reserving a clear liquid part; purifying the filtrate treated by the clarifying agent through a macroporous resin column; concentrating the effluent of the macroporous resin column to obtain a crude product concentrated solution; diluting the crude product concentrated solution with an organic solvent; adsorbing and purifying by using Fe3O4 magnetic nanoparticles; taking the clear liquid part to pass through a chromatographic column for chromatography; and carrying out vacuum concentration on the chromatographic solution, and carrying out vacuum freeze drying to obtain pure ecdysterone after drying. According to the method, ecdysterone with the content of 98% or above can be obtained, so that the product content is increased, the product purity and yield are greatly increased, ethyl alcohol and ethyl acetate used in the production process can be recycled according to the boiling points of ethyl alcohol and ethyl acetate, and the product extracted through the method almost has no solvent residues, is higher in purity and is safer.

The invention discloses feed for enhancing the constitution of young river crabs. The raw materials of the feed comprise puffed corn flour, fermented bean meal, broad bean flour, pumpkin flour, wheatbran, seaweed meal, feather meal, shrimp meal, silkworm powder, animal visceral meal, green tea powder, vegetable powder, saccharides, ecdysterone, Yingkesu, citric acid, tartaric acid, succinic acid,casein phosphopeptide, a complex enzyme preparation, Chinese herbal medicine additives, vegetable oil and table salt. The feed for enhancing the constitution of young river crabs, disclosed by the invention, is reasonable in formula, can be easily digested and absorbed by the young river crabs, has a small feed coefficient and a good health-care effect, effectively enhances the constitution of the young crabs, reduces the morbidity of the young crabs, enables the young crabs to grow and develop quickly and have low mortality, and effectively improves the economic benefit of river crab breeding.

The invention provides a method for determining multi-component content of a Jingyaokang capsule. The method comprises the following steps: S1, preparation of a mixed reference substance solution, precisely weighing strychnine, alpha-cypress alkali, periplocin, 11-carbonyl-beta-frankincense acid, curcuma zedoary bicyclo-enone, hydroxysafflor yellow A, naringin, tetrandrine, fangchinoline and beta-ecdysterone respectively, and dissolving the above materials in a solvent to obtain the mixed reference substance solution for later use; s2, preparation of a test solution, taking the Jingyaokang capsule, adding a solvent for extraction, performing filtering, and taking filtrate to obtain the test solution for later use; s3, content determination, precisely sucking the reference substance solution and the test solution respectively, injecting the solutions into a liquid chromatograph, and performing determining to obtain the content. According to the determination method, the contents of tenchemical components such as strychnine, alpha-jacosinine, periplocin, 11-carbonyl-beta-masticinic acid and naringin in the Jingyaokang capsule are determined, and the quality of the Jingyaokang capsule can be better controlled.