7 results about "Reference product" patented technology

The application discloses a method for detecting specific activity of recombinant Pseudomonas aeruginosa vaccine rPO stock solution, and comprises the following steps: (1) using OprI-B052 as a capture antibody to coat an enzyme-labeled plate; (2) adding a sample to be detected into the coated enzyme-labeled plate; (3) diluting an enzyme-labeled antibody PcrV-A039 and then performing a color reaction to measure an OD 450 / 630 nm value; and (4) preparing a standard curve by using a rPO reference product, and calculating the specific activity of the rPO antigen in the sample according to the standard curve. In the application, OprI-B052 is used as a coating antibody, and PcrV-A039 is used as a detection antibody. The specific activity of the recombinant Pseudomonas aeruginosa vaccine rPO stock solution can be quantitatively detected, and the application has good stability and repeatability.

This invention relates to the fields of molecular biology and human genetic disease detection; specifically, it relates to a reference standard for methylmalonic acidemia and its application. The reference standard comprises positive reference cells with MMACHC gene mutation sites. The genome of the positive reference cells contains one or more combinations of specific mutation sites of the following MMACHC genes: c.609G>A, c.567insT, c.658_660delAAG, c.482G>A, c.1A>G, c.80A>G, c.217C>T, c.315C>G, and c.394C>T. This reference standard provides reliable technical support for the accurate detection of methylmalonic acidemia, especially MMACHC gene-related mutations, and is of great significance in improving detection accuracy and promoting detection standardization.

THE method for imprinting an active effect of at least one reference product into a treatment PRODUCT INCLUDES a second imprinting phase (E2) comprising: —reading (E21) the associated digital signature of the at least one reference product from a database; —forming (E23) an electrical imprinting signal from the digital signature of the reference product; —imprinting (E24) the active effect into a neutral solution HAVING at least one salt, by passing the electrical imprinting signal through one or more electrical wires of a plate of an imprinting device on which the neutral solution is positioned, for a predetermined imprinting duration, so as to imprint the active effect of the at least one reference product into the neutral solution and thus transform it into a treatment product.

The application discloses a kind of apple bruise resistance dynamic detection and grading method based on solid-liquid decoupling, belong to agricultural product test technical field.The method includes: (1) based on the same library reference product generates dynamic judgment threshold, namely apparent density determination threshold ρ th And hysteresis loss rate determination threshold H th , construct two-dimensional evaluation matrix;(2) extract apple sample from the batch to be measured, determine apparent density ρ And hysteresis loss rate under high strain rate HLR;(3) the measured data is mapped to two-dimensional evaluation matrix to grade the fruit of the batch to be measured, and then trigger the corresponding physical shunt and intervention instruction.Compared with the existing single detection technology that relies on static hardness, the application utilizes the solid-liquid intrinsic decoupling characteristics of the flesh porous medium, converts the apparent density representing the strength of the solid skeleton and the HLR representing the viscous dissipation ability of the fluid into a two-dimensional orthogonal judgment signal, achieving precise identification and automatic damage prevention intervention of different damage failure modes.

The application discloses a flavone bioactivity evaluation method based on molecular docking and digestion simulation, and belongs to the technical field of natural product activity evaluation. After a flavone sample to be measured is subjected to INFOGEST standard in-vitro digestion simulation, small intestine stage product supernatant is collected. The product types and abundance are identified by using liquid chromatography tandem mass spectrometry, and the product is subjected to double-parameter coupling screening of lipid membrane penetration determination and rapid rigid docking pre-screening to determine the main active form. The main active form is subjected to fine semi-flexible molecular docking with intestinal local target proteins to obtain affinity data. Based on a competitive occupation nonlinear model, an activity index is calculated by using the abundance and affinity competition normalized weight of each product to replace simple linear weighting. According to the activity index and reference product threshold comparison, the activity level is divided. The method overcomes the defects of mutual fragmentation of digestion simulation and molecular docking and linear superposition evaluation in the prior art through function coupling and competition correction, and improves the precision of flavone oral activity evaluation.

The application discloses a method for quantitatively detecting specific activity of HI antigen in recombinant staphylococcus aureus vaccine or stock solution, comprising the following steps: (1) coating an enzyme-labeled plate with IsdB-318 as a capture antibody; (2) adding a sample to be measured into the coated enzyme-labeled plate; (3) performing color development reaction by using an enzyme-labeled Hla-26 monoclonal antibody, and measuring OD 450 / 630 nm value; and (4) preparing a standard curve by using an HI reference product, and calculating specific activity of the HI antigen in the sample according to the standard curve. The application can quantitatively detect specific activity of the HI protein in the recombinant staphylococcus aureus vaccine or stock solution, and has good stability and repeatability.