18 results about "Reference product" patented technology

The application discloses a single-tube quantitative detection HBV viral load real-time fluorescent quantitative PCR kit and belongs to the technical field of molecular biological detection. The kit comprises a primer probe combination for single-tube quantitative detection HBV viral load real-time fluorescent quantitative PCR, wherein the primer probe combination comprises a primer pair and a probe for detecting a target gene, and a probe IC-P1 and a probe IC-P2 for detecting an internal standard. The kit can cover various genotypes of HBV viruses, and the minimum detectable viral load is 2.5 IU / mL, the detection sensitivity is high, the repeatability is good, and the quantitative standard curve is constructed without additional quantitative reference products, so that the operation is simplified and the utilization rate of the reagent is improved.

The application discloses a single-tube quantitative detection HCV viral load real-time fluorescent quantitative PCR kit and belongs to the technical field of molecular biological detection. The kit comprises a pair of primers and a probe combination, the primer and the probe combination comprise a primer pair and a probe for detecting a target gene and a probe for detecting an internal standard. The kit can detect HCV 1-6 common types in clinic, covers all types, can stably detect a viral load of 10 IU / mL, has high detection sensitivity and good repeatability, does not need additional quantitative reference product to construct a calibration curve, simplifies operation, improves the utilization rate of reagents, and avoids invalid results and repeated detection caused by the failure of calibration curve construction.

The application discloses a method for detecting specific activity of recombinant Pseudomonas aeruginosa vaccine rPO stock solution, and comprises the following steps: (1) using OprI-B052 as a capture antibody to coat an enzyme-labeled plate; (2) adding a sample to be detected into the coated enzyme-labeled plate; (3) diluting an enzyme-labeled antibody PcrV-A039 and then performing a color reaction to measure an OD 450 / 630 nm value; and (4) preparing a standard curve by using a rPO reference product, and calculating the specific activity of the rPO antigen in the sample according to the standard curve. In the application, OprI-B052 is used as a coating antibody, and PcrV-A039 is used as a detection antibody. The specific activity of the recombinant Pseudomonas aeruginosa vaccine rPO stock solution can be quantitatively detected, and the application has good stability and repeatability.

The application discloses a single-tube quantitative detection HIV-1 virus load real-time fluorescent quantitative PCR kit and relates to the technical field of molecular biology detection. The kit comprises a primer and probe combination; the primer and probe combination comprises a primer pair GAG-1-F / R and a probe GAG-1-P for detecting a first target gene, a primer pair POL-1-F / R and a probe POL-1-P for detecting a second target gene, and probes IC-P1 and IC-P2 for detecting an internal standard. The kit covers various genotypes of HIV-1 viruses, can stably detect a virus load of 15 IU / mL, has high detection sensitivity and good repeatability, does not need additional quantitative reference products to construct a quantitative standard curve, can simplify operations, and improves the utilization rate of reagents.

The present application relates to a kind of kits for detecting sows' nutritional iron deficiency by biomarker quantitative detection evaluation and application, including fluorescent reaction liquid A, fluorescent reaction liquid B, internal standard, negative control, nucleic acid release agent and quantitative reference product, the fluorescent reaction liquid B contains detection primer and probe, the detection primer includes upstream primer and downstream primer, the sequence of upstream primer is 5'-ATCTGGCTTGTAAACCAACA-3', the sequence of downstream primer is 5'-ATCTTAGTTGTGGATTCCGT-3', the fluorescent reaction liquid B also contains primer probe, probe sequence is 5'-TCACCGTCAATTTCGGCTCT-3', 5' end is labeled with reporter fluorescent group, and 3' end is labeled with quenching fluorescent group.The present application effectively improves the accuracy of sows' nutritional iron deficiency detection, and provides scientific guidance for sow nutrition.

This invention relates to the fields of molecular biology and human genetic disease detection; specifically, it relates to a reference standard for methylmalonic acidemia and its application. The reference standard comprises positive reference cells with MMACHC gene mutation sites. The genome of the positive reference cells contains one or more combinations of specific mutation sites of the following MMACHC genes: c.609G>A, c.567insT, c.658_660delAAG, c.482G>A, c.1A>G, c.80A>G, c.217C>T, c.315C>G, and c.394C>T. This reference standard provides reliable technical support for the accurate detection of methylmalonic acidemia, especially MMACHC gene-related mutations, and is of great significance in improving detection accuracy and promoting detection standardization.

The present invention provides a topical pharmaceutical formulation comprising a combination of miconazole or a pharmaceutically acceptable salt thereof and domiphen bromide, a formulation vehicle and a viscosity modifier; wherein the ratio of w / w% miconazole (salt) to w / w% domiphen bromide is between 6 to 20 endpoints included and wherein the amount of viscosity modifier is sufficient to adjust the viscosity of the topical formulation to a value of 1000-4600 mPa.s measured at 25°C. The invention further provides use of said formulation in a treatment of vaginal Candida (biofilm) infections, wherein once daily use of 4.5-5.5 g of the formulation for a duration of 7 days performed better than a reference product and unexpectedly also performed better than a formulation with a double domiphen bromide dose. Improved performance was the relapse prevention for a duration of at least 28 days in human patients treated for a vulvovaginal Candida (biofilm) infection.

The present invention relates to a method for imprinting the active effect of at least one reference product into a processed product, having a second imprint phase (E2) comprising the following steps: reading (E21) an associated digital signature of at least one reference product from a database; forming (E23) an electrical imprint signal from the digital signature of the reference product; imprinting (E24) the active effect into a neutral solution containing at least one salt, by passing the electrical imprint signal through one or more electrical wires of a plate of an imprinting device in which the neutral solution is located, thereby imprinting the active effect of the at least one reference product into the neutral solution over a predetermined imprint period, thereby converting the neutral solution into the processed product.

THE method for imprinting an active effect of at least one reference product into a treatment PRODUCT INCLUDES a second imprinting phase (E2) comprising: —reading (E21) the associated digital signature of the at least one reference product from a database; —forming (E23) an electrical imprinting signal from the digital signature of the reference product; —imprinting (E24) the active effect into a neutral solution HAVING at least one salt, by passing the electrical imprinting signal through one or more electrical wires of a plate of an imprinting device on which the neutral solution is positioned, for a predetermined imprinting duration, so as to imprint the active effect of the at least one reference product into the neutral solution and thus transform it into a treatment product.

The invention relates to the technical field of tourism product management, in particular to a tourism product advertisement putting method and system based on big data. Marking the tourism products which have been opened as played products, and obtaining product labels corresponding to the played products; then, marking a currently logged-in user as a current user, matching a current label of the current user with a client label of a traveled tourist of each traveled product to obtain a first matching value of each traveled product relative to the current user, and marking the traveled product with the highest first matching value as a reference product of the current user; all other tourism products except the reference product are marked as to-be-selected products of the current user, and second matching values of other to-be-selected products relative to the reference product are further obtained based on the reference product; and then the to-be-selected products are arranged according to the descending order of the second matching values and are combined with the reference product to obtain a promotion sequence, so that the advertisement putting accuracy is improved.

A system (102) and a method (300) for planning an assembly sequence by comparing a new product series and a reference product series. The method includes receiving a first set of attributes corresponding to the new product series. The method includes determining a second set of attributes corresponding to the reference product series. Using an exact matching operation for a candidate part of the new product series, the method includes comparing the part number of the candidate part with the reference part number and the part description of the candidate part with the reference part description. This can be done based on the match between the submodule number of the candidate part and the reference submodule number. In response to the detection of a mismatch during the exact matching operation, the method includes generating a vector representation of the part description corresponding to the candidate part.Furthermore, the procedure includes retrieving, for the appropriate candidate part, a complete contextual assignment from the reference series for the new series that meets a predefined threshold, in order to plan the assembly sequence.

A system (102) and a method (300) for planning a new product series using a reference product series are disclosed. The system (102) collects a first set of attributes corresponding to the new product series and a second set of attributes corresponding to the reference product series, and compares the first set of attributes with the second set of attributes using an anchoring operation. The system (102) identifies a candidate set of attributes and determines a semantic vector embedding for each attribute of the candidate set using a trained embedding model to generate an embedded set of attributes. The system (102) iteratively implements a gap-filling operation to identify a suitable interval between the generated embedded set of attributes.The system (102) iteratively performs a generative validation process to evaluate the generated embedded set of attributes using predefined contextual prompts in order to complete the planning of the new series using the reference series.

The invention discloses a method for determining allylamine in a sevelamer carbonate medicine. The method comprises the following steps: (1) preparing a sevelamer carbonate drug test solution and an allylamine reference solution; (2) respectively carrying out derivatization treatment on the test solution and the reference solution, and during derivatization treatment, adjusting the pH value of a derivatization system by using a buffer solution with the pH value of 8.4-10.8; and (3) determining the derivatization system by using a high performance liquid chromatography. The sevelamer carbonate drug is alkaline, so that the pH value of a test product reaction system is high, the pH value is greatly different from the pH value of a reference product reaction system, the analysis result is unstable, the pH values of a test product derivative system and a reference product derivative system are adjusted to tend to be consistent by adding a buffer solution, so that the influence of the pH value difference on the analysis result is reduced, and the accuracy of the analysis result is improved. The accuracy, repeatability and stability of analysis are improved, the requirements and standards of pharmacopeia on the analysis method are better met, and quality control and safe production of sevelamer carbonate drugs are facilitated.

The present application relates to the technical field of tourism product management, and particularly relates to a tourism product advertisement putting method and system based on big data; a tourism product that has been opened is marked as a played product, and product tags corresponding to each played product are obtained; then a currently logged-in user is marked as a current user, a current tag of the current user and customer tags of played tourists of each played product are matched to obtain a first matching value of each played product relative to the current user, a played product with the highest first matching value is marked as a reference product of the current user, and all other tourism products except the reference product are marked as candidate products of the current user, and a second matching value of other candidate products relative to the reference product is further obtained based on the reference product; then each candidate product is arranged in descending order of the second matching value and combined with the reference product to obtain a promotion sequence, so as to improve the accuracy of advertisement putting.

The invention provides a product retrieval method and device based on a large language model, and the method comprises the steps: obtaining a query text, inputting the query text into a first large language model, carrying out the reasoning of the first large language model based on the query text, and generating the identification information of a reference product; retrieving in a real-time database based on the identification information of the reference product to obtain real-time related information of the reference product from the real-time database; wherein the real-time database is used for storing the corresponding relationship between the identification information of the product and the real-time related information; and determining identification information of a target product related to the query text based on the query text, the identification information of the reference product and real-time related information.

The application discloses a method for quantitatively detecting specific activity of HI antigen in recombinant staphylococcus aureus vaccine or stock solution, comprising the following steps: (1) coating an enzyme-labeled plate with IsdB-318 as a capture antibody; (2) adding a sample to be measured into the coated enzyme-labeled plate; (3) performing color development reaction by using an enzyme-labeled Hla-26 monoclonal antibody, and measuring OD 450 / 630 nm value; and (4) preparing a standard curve by using an HI reference product, and calculating specific activity of the HI antigen in the sample according to the standard curve. The application can quantitatively detect specific activity of the HI protein in the recombinant staphylococcus aureus vaccine or stock solution, and has good stability and repeatability.