48 results about "Reference product" patented technology

The invention provides a non-invasive fetal trisomy 21 syndrome prenatal screening kit based on digital PCR, and relates to the technical field of fetal trisomy 21 syndrome screening, according to a method for enriching fetal cfDNA in pregnant woman plasma, SPRIselect magnetic beads with low enrichment multiple and silicon hydroxyl magnetic beads are combined for use, and under a certain dosage, the recovery rate of fetal cfDNA short fragments reaches 95%; according to the provided multiple system construction method, positive gDNA and negative gDNA are obtained on the basis of a 21 trisomy positive cell line and a 21 trisomy negative cell line, low-proportion reference substances are prepared and used, internal reference sites and detection sites which are high in detection capacity and stable in detection performance are screened from the beginning, and compared with existing multiple system establishment and verification through direct use of pregnant woman plasma samples, the multiple system construction method has the advantages that the detection efficiency is high, and the detection cost is low. The to-be-detected sample with lower fetal cfDNA content can be stably detected.

The application discloses a reagent, a method and application of the reagent and the method for simultaneously detecting three common viruses in a fancy carp, and belongs to the technical field of fish quarantine. The application provides a reaction system and a detection method for simultaneously detecting three common viruses in a fancy carp, establishes a multiplex fluorescence PCR method, adopts a full-closed reaction, solves the problem that common PCR is prone to generating aerosols and causing false positive results of tests, has high detection sensitivity, can detect target genes with a copy number of 10 orders of magnitude at the minimum, is suitable for detecting a small amount of viruses carried in normal fancy carp or viruses in water quality environment, and can fully meet the requirements of customs quarantine prevention and control, has strong specificity, has no cross reaction to templates such as IHNV, RSIV and EHNV, and further provides a virus-like particle which can be used as a positive reference product in the multiplex system, is stable in state and beneficial to storage, can monitor an extraction process, and ensures that a test is established.

The application discloses a single-tube quantitative detection HBV viral load real-time fluorescent quantitative PCR kit and belongs to the technical field of molecular biological detection. The kit comprises a primer probe combination for single-tube quantitative detection HBV viral load real-time fluorescent quantitative PCR, wherein the primer probe combination comprises a primer pair and a probe for detecting a target gene, and a probe IC-P1 and a probe IC-P2 for detecting an internal standard. The kit can cover various genotypes of HBV viruses, and the minimum detectable viral load is 2.5 IU / mL, the detection sensitivity is high, the repeatability is good, and the quantitative standard curve is constructed without additional quantitative reference products, so that the operation is simplified and the utilization rate of the reagent is improved.

The application provides a method for preparing FFPE quality control products by using cell lines, a reference product and a kit, and belongs to the fields of medicine, clinical laboratory science and biotechnology, and comprises the following steps: culturing cell lines by using microcarriers; mixing different cell lines based on requirements; centrifuging the mixed cell microcarrier suspension in a sealed malt pipe at one end; removing supernatant after the treatment; packaging the two ends of the malt pipe by using a semi-permeable membrane; removing the semi-permeable membrane after fixing, dehydrating and transparentizing the malt pipe by using a formaldehyde solution; separating the cell microcarriers after the wax immersion treatment from the malt pipe; and embedding the cell microcarriers into a paraffin block by using wax. The quality control product can be used in subsequent quality control processes, the mutation frequency of the quality control product can be kept in good uniformity, and batch production and large-scale production of the quality control product can be realized.

The present disclosure relates to a method and apparatus for determining product replenishment quantities. The method for determining product replenishment quantities includes: obtaining historical data of multiple reference products related to a target product; determining attribute data of product attributes of each reference product based on its historical data, wherein the product attributes include demand attributes, and the attribute data of the demand attributes include the historical demand of the reference product; clustering the multiple reference products based on the attribute data of the product attributes of each reference product and target values ​​of the attribute weights to obtain multiple reference classes, wherein the attribute weights include demand attribute weights; screening reference classes similar to the target product from the multiple reference classes as similar classes; predicting the future demand of the target product in a future cycle based on the attribute data of the demand attributes of each reference product in the similar class; and determining the future replenishment quantity of the target product in the future cycle based on the future demand of the target product.

The invention discloses a blood chyle degree detection reference and a preparation method thereof. The reference comprises the following components: triglyceride, cholesteryl ester, free cholesterol, phosphatidylcholine, bovine serum albumin, a buffer solution and an auxiliary agent. The blood chyle degree detection reference product provided by the invention can be stably stored, is low in cost, can be used for instrument calibration, can be used for judging and grading clinical plasma chyle degree and the like, and can unify detection standards and eliminate artificial interference; based on the blood chyle degree detection reference product provided by the invention, detection can be realized by utilizing image gray calculation, and compared with manual visual inspection, the detection accuracy is greatly improved.

The present application discloses a method, device, server, and storage medium for updating classification status, belonging to the field of the Internet. The method includes: determining that the status of a product corresponding to a classification has changed; in the case where the status of the product changes from being on the shelf to being off the shelf, if the product is not the reference product corresponding to the classification, then the status of the classification is not updated; if the product is the reference product corresponding to the classification and there is no corresponding reference product on the shelf for the classification, then the status of the classification is updated according to the status of each product corresponding to the classification; if the product is the reference product corresponding to the classification and there is a corresponding reference product on the shelf for the classification, then the status of the classification is not updated; in the case where the status of the product changes from being off the shelf to being on the shelf, if there is no corresponding reference product on the shelf for the classification, then the status of the classification is updated, and if there is a corresponding reference product on the shelf for the classification, then the status of the classification is not updated. By adopting the present application, the classification update efficiency can be improved.

The invention discloses a method for preparing a sheep-derived positive serum reference of yersinia enterocolitica, obtained positive serum and application of the positive serum, and belongs to the field of veterinary medicine products. The invention aims to solve the technical problem of how to obtain the yersinia enterocolitica sheep-derived positive serum reference which is stable in titer and character and can be used for specific detection of brucella diagnostic products. Therefore, the invention provides a method for preparing the yersinia enterocolitica sheep-derived positive serum reference, which comprises the following steps: immunizing a tested sheep by using an immunizing antigen, and collecting serum of the immunized tested sheep to obtain the yersinia enterocolitica sheep-derived positive serum reference. The immune antigen is an inactivated yersinia enterocolitica obtained by inactivating the yersinia enterocolitica. The positive serum reference can be effectively applied to quality control evaluation work of brucellosis related diagnosis products, and plays a positive role in prevention and control of brucellosis.

The application discloses a single-tube quantitative detection HCV viral load real-time fluorescent quantitative PCR kit and belongs to the technical field of molecular biological detection. The kit comprises a pair of primers and a probe combination, the primer and the probe combination comprise a primer pair and a probe for detecting a target gene and a probe for detecting an internal standard. The kit can detect HCV 1-6 common types in clinic, covers all types, can stably detect a viral load of 10 IU / mL, has high detection sensitivity and good repeatability, does not need additional quantitative reference product to construct a calibration curve, simplifies operation, improves the utilization rate of reagents, and avoids invalid results and repeated detection caused by the failure of calibration curve construction.

The application discloses a method for detecting specific activity of recombinant Pseudomonas aeruginosa vaccine rPO stock solution, and comprises the following steps: (1) using OprI-B052 as a capture antibody to coat an enzyme-labeled plate; (2) adding a sample to be detected into the coated enzyme-labeled plate; (3) diluting an enzyme-labeled antibody PcrV-A039 and then performing a color reaction to measure an OD 450 / 630 nm value; and (4) preparing a standard curve by using a rPO reference product, and calculating the specific activity of the rPO antigen in the sample according to the standard curve. In the application, OprI-B052 is used as a coating antibody, and PcrV-A039 is used as a detection antibody. The specific activity of the recombinant Pseudomonas aeruginosa vaccine rPO stock solution can be quantitatively detected, and the application has good stability and repeatability.

1. Name of the product in this design: Packaging Box (Class III Gift Box). 2. Purpose of this design: For use as the outer packaging box for reference products. 3. The key design features of this product are the combination of pattern and color. 4. The image or photograph that best illustrates the design's key points: a 3D model. 5. The design for which protection is sought includes color.

The application discloses a single-tube quantitative detection HIV-1 virus load real-time fluorescent quantitative PCR kit and relates to the technical field of molecular biology detection. The kit comprises a primer and probe combination; the primer and probe combination comprises a primer pair GAG-1-F / R and a probe GAG-1-P for detecting a first target gene, a primer pair POL-1-F / R and a probe POL-1-P for detecting a second target gene, and probes IC-P1 and IC-P2 for detecting an internal standard. The kit covers various genotypes of HIV-1 viruses, can stably detect a virus load of 15 IU / mL, has high detection sensitivity and good repeatability, does not need additional quantitative reference products to construct a quantitative standard curve, can simplify operations, and improves the utilization rate of reagents.

The invention provides a yield prediction method and device of a produced product, a storage medium and a program product, and relates to the technical field of data processing, and on the basis of obtaining reference product information of each reference product and target product information of a target product, clustering processing is performed on each reference product to obtain at least two reference product clustering sets; and for each reference product cluster set, performing multi-head self-attention fusion processing to generate a corresponding product relation degree matrix. And matching a corresponding target reference product cluster set according to the target product information of the target product, and determining the target reference product. In each production time window, training the initial yield prediction model to obtain a target yield prediction model; inputting the product relation degree matrix corresponding to the target reference product cluster set, the target product information of the target product and the future time feature vector into a full-connection neural network layer, and outputting a target predicted yield; the problem of inaccurate yield prediction of newly developed products is solved.

The present application relates to a kind of kits for detecting sows' nutritional iron deficiency by biomarker quantitative detection evaluation and application, including fluorescent reaction liquid A, fluorescent reaction liquid B, internal standard, negative control, nucleic acid release agent and quantitative reference product, the fluorescent reaction liquid B contains detection primer and probe, the detection primer includes upstream primer and downstream primer, the sequence of upstream primer is 5'-ATCTGGCTTGTAAACCAACA-3', the sequence of downstream primer is 5'-ATCTTAGTTGTGGATTCCGT-3', the fluorescent reaction liquid B also contains primer probe, probe sequence is 5'-TCACCGTCAATTTCGGCTCT-3', 5' end is labeled with reporter fluorescent group, and 3' end is labeled with quenching fluorescent group.The present application effectively improves the accuracy of sows' nutritional iron deficiency detection, and provides scientific guidance for sow nutrition.

This invention relates to the fields of molecular biology and human genetic disease detection; specifically, it relates to a reference standard for methylmalonic acidemia and its application. The reference standard comprises positive reference cells with MMACHC gene mutation sites. The genome of the positive reference cells contains one or more combinations of specific mutation sites of the following MMACHC genes: c.609G>A, c.567insT, c.658_660delAAG, c.482G>A, c.1A>G, c.80A>G, c.217C>T, c.315C>G, and c.394C>T. This reference standard provides reliable technical support for the accurate detection of methylmalonic acidemia, especially MMACHC gene-related mutations, and is of great significance in improving detection accuracy and promoting detection standardization.

The invention relates to a glycosylation-like extracellular vesicle as well as a preparation method and application thereof. The invention relates to a glycosylated lipidosome which comprises a glycosylated lipidosome outer membrane and nucleic acid wrapped in the glycosylated lipidosome outer membrane, and the glycosylated lipidosome outer membrane comprises cholesterol, phospholipid, cationic lipid and an amphiphilic lipid-PEG-glycosyl compound in a molar ratio of (6-10): (6.5-4.5): (6.5-4.5): 1. According to the method, the glycosylated amphiphilic lipid, the artificially synthesized glycosylated liposome and the simulated glycosylated EVs subgroup are used as a reference substance or a quality control substance of the glycosylated EVs, so that the problem that an existing sugar capture method for extracting the glycosylated EVs subgroup is lack of quality control standards can be solved.

The present specification provides an alarm processing method and device, the method comprises: acquiring a query text, and inputting the query text into a first large language model; generating identification information of a reference product based on the query text by the first large language model; searching in a real-time database based on the identification information of the reference product to obtain real-time related information of the reference product from the real-time database; wherein the real-time database is used to store the corresponding relationship between the identification information and the real-time related information of the product; based on the query text, the identification information and the real-time related information of the reference product, the identification information of the target product related to the query text is determined.

A method of identity determination based on artificial intelligence is disclosed. The method may include: acquiring a first image of a reference product; acquiring a second image of a comparison target product which is a comparison target of the reference product; acquiring a plurality of first features corresponding to the first image and a plurality of second features corresponding to the second image by inputting the first image and the second image to a feature extraction model; generating a plurality of feature pairs by matching the plurality of first features and the plurality of second features; acquiring a similarity score for each of the plurality of feature pairs by inputting two features constituting each of the plurality of feature pairs to a similarity analysis model; and determining an identity between the reference product and the comparison target product based on the similarity score.

The invention relates to a product recommendation method and device, computer equipment and a readable storage medium. The method comprises the following steps: receiving a product recommendation request; the product recommendation request comprises a target reference product identifier; the target reference product identifier is a product identifier of a target reference product determined based on the user preference information; according to the product type of a target reference product corresponding to the target reference product identifier, obtaining each candidate recommended product from the recommended product set; determining a target candidate recommended product sequence according to the reference product feature data set of the target reference product, the recommended product feature data set corresponding to each candidate recommended product and a cosine similarity algorithm; and obtaining user feature data of the to-be-recommended user, and screening a target recommended product from the target candidate recommended product sequence according to the user feature data. By adopting the method, the accuracy of the product recommendation method can be improved.

The present invention provides a topical pharmaceutical formulation comprising a combination of miconazole or a pharmaceutically acceptable salt thereof and domiphen bromide, a formulation vehicle and a viscosity modifier; wherein the ratio of w / w% miconazole (salt) to w / w% domiphen bromide is between 6 to 20 endpoints included and wherein the amount of viscosity modifier is sufficient to adjust the viscosity of the topical formulation to a value of 1000-4600 mPa.s measured at 25°C. The invention further provides use of said formulation in a treatment of vaginal Candida (biofilm) infections, wherein once daily use of 4.5-5.5 g of the formulation for a duration of 7 days performed better than a reference product and unexpectedly also performed better than a formulation with a double domiphen bromide dose. Improved performance was the relapse prevention for a duration of at least 28 days in human patients treated for a vulvovaginal Candida (biofilm) infection.

The invention discloses a method for quantitatively detecting the specific activity of a recombinant pseudomonas aeruginosa vaccine rTF stock solution. The method comprises the following steps: S1, coating an elisa plate by taking FliC-16G10 as a capture antibody; s2, adding a sample to be detected into the coated elisa plate; s3, incubating the enzyme-labeled antibody TonB-8E10, carrying out color development by using a TMB (Tetramethylbenzidine) color development solution, and reading OD (Optical Density) values of 450 nm and 630 nm by using a microplate reader; and S4, drawing a standard curve by using the rTF reference substance, and calculating the specific activity of the rTF stock solution in the detected sample according to the standard curve. According to the method, Flic-16G10 is used as a capture antibody for coating, and a sample with unknown antigen activity concentration is quantitatively detected through an obtained linear interval, so that the relationship between the antigen protein concentration and the antigen activity concentration is obtained. The specific activity of the recombinant pseudomonas aeruginosa vaccine rTF stock solution can be quantitatively detected, and the method has good stability and repeatability.

The present invention relates to a method for imprinting the active effect of at least one reference product into a processed product, having a second imprint phase (E2) comprising the following steps: reading (E21) an associated digital signature of at least one reference product from a database; forming (E23) an electrical imprint signal from the digital signature of the reference product; imprinting (E24) the active effect into a neutral solution containing at least one salt, by passing the electrical imprint signal through one or more electrical wires of a plate of an imprinting device in which the neutral solution is located, thereby imprinting the active effect of the at least one reference product into the neutral solution over a predetermined imprint period, thereby converting the neutral solution into the processed product.

The invention discloses a method for quantitatively detecting specific activity of a SpA5 antigen in a recombinant staphylococcus aureus vaccine or / and a stock solution. The method comprises the following steps: S1, coating an elisa plate by taking SpA5-H3 as a capture antibody; s2, adding a sample to be detected into the coated elisa plate; s3, incubating an enzyme-labeled antibody SpA5-16, developing by using a TMB (Tetramethylbenzidine) developing solution, respectively detecting OD (Optical Density) values at 450 nm and 630 nm by using an enzyme labeling instrument, and calculating an OD (Optical Density) value of 450-630 nm; and S4, drawing a standard curve by using the SpA5 reference substance, and calculating the specific activity of the SpA5 antigen in the detected sample according to the standard curve. A sample with unknown antigen activity concentration is quantitatively detected by the method provided by the invention, so that the antigen activity concentration is obtained. The specific activity of the SpA5 antigen in a recombinant staphylococcus aureus vaccine or / and a stock solution can be quantitatively detected, and good stability and repeatability are achieved.

A method of automatically matching target product data to reference product data, the method includes, via a server system, retrieve target product data for a target product that includes target product price and interaction data. The interaction data corresponds to a number of interactions with a rendering of the target product at a target user interface (UI). A web-scraping frequency is determined based on the interaction data. Web-scraping of a reference UI is executed to retrieve reference product data corresponding to a reference product according to the web-scraping frequency. The reference product data includes a reference product price. The target product data is matched to the reference product data. The target product is automatically rendered with the new target product price at the target UI.

The invention belongs to the technical field of gene detection, and particularly relates to a lung cancer methylation gene tag, a lung cancer methylation enterprise reference and application of the lung cancer methylation gene tag and the lung cancer methylation enterprise reference. The lung cancer methylation enterprise reference comprises a positive cell line DNA sample, a negative cell line DNA sample, a reagent for methylating DNA and a specific primer for amplifying a target gene, and the target genes are CDO1, SHOX2, PTGER4 and H4C6. The invention further discloses a method for preparing the target gene. The invention provides a group of gene tags highly associated with the methylation level of the lung cancer, the enterprise reference provided by the invention can truly reflect the methylation levels of all CpG sites in a target area, the quantitative method is simple, the operation time can be greatly shortened, the experimental process is simplified, the experimental cost is reduced, and the application prospect is very good.

A method of automatically matching target product data to reference product data, the method includes, via a server system, retrieve target product data for a target product that includes target product price and interaction data. The interaction data corresponds to a number of interactions with a rendering of the target product at a target user interface (UI). A web-scraping frequency is determined based on the interaction data. Web-scraping of a reference UI is executed to retrieve reference product data corresponding to a reference product according to the web-scraping frequency. The reference product data includes a reference product price. The target product data is matched to the reference product data. The target product is automatically rendered with the new target product price at the target UI.

The present invention discloses a method for detecting the hemagglutination titer of an influenza virus antigen sample, comprising the following steps: S1. Lysis treatment: adding a lysing agent to the influenza virus antigen sample and the influenza virus antigen hemagglutination titer reference product respectively for lysis to obtain a lysed influenza virus antigen sample and a lysed influenza virus antigen hemagglutination titer reference product; the influenza virus antigen sample is an intermediate produced in each process during the production of the monovalent bulk of the influenza virus split vaccine; S2. Hemagglutination titer detection: detecting the hemagglutination titer of the lysed influenza virus antigen sample and the hemagglutination titer of the lysed influenza virus antigen hemagglutination titer reference product respectively; S3. Calculating the standard value of the hemagglutination titer of the influenza virus antigen sample. The detection method of the present application has good precision, good repeatability and good accuracy, and can be used for the quality control evaluation of intermediates in each process during the production of the monovalent bulk of the influenza virus split vaccine.

The present invention provides a urine simulation sample, a reference product for urothelial carcinoma, and a preparation method and application thereof. A preparation method of a urine simulation sample for urothelial carcinoma comprises the following steps: S1: preparing a urothelial carcinoma cell culture: digesting T24 cells with trypsin at a concentration of 0.15% - 0.35% v / v, and culturing to obtain a T24 cell suspension; S2: preparing free DNA of urothelial carcinoma: fragmenting the human genomic DNA standard NA12878 with 100% methylation level into free DNA with a length of 150 bp - 300 bp; S3: mixing the urothelial carcinoma cell culture obtained in step S1, the free DNA of urothelial carcinoma obtained in step S2, and a urine sample of a healthy person to obtain a urine simulation sample for urothelial carcinoma. The present invention provides a urine simulation sample and a reference product with a clear background, capable of simulating clinical samples, having low cost, being renewable and having universality.

The present invention discloses a method for preparing a mutant gene reference product, its product and application. When there are n mutation sites in the gene to be detected, n mutant gene fragments of the gene sequence containing n mutation sites are used as n mutant gene sequences, and their corresponding n wild-type gene fragments are used as n wild-type gene sequences; 2) Obtaining of random sequences; 3) Selection of quantitative sequences; 4) Designing n sequence fragments by spacing n wild-type gene sequences, n mutant gene sequences and random sequences in sequence, connecting the n sequence fragments with random sequences to obtain a sequence, and connecting 1 quantitative sequence at the end of the sequence to obtain the mutant gene reference product. By designing a group or multiple groups of wild sequences and mutant sequences on the same sequence and separating them with irrelevant sequences, the present invention can make the theoretical ratio of wild / mutant sequences completely consistent with the actual ratio, and effectively avoid the errors and risks brought by nucleic acid degradation.

The invention discloses a method for preparing a yersinia enterocolitica bovine-derived positive serum reference and the obtained bovine-derived positive serum reference, and belongs to the technical field of veterinary biological products. The invention aims to solve the technical problem of how to obtain the yersinia enterocolitica bovine-derived positive serum reference which is stable in titer and character and can be used for specific detection of brucella diagnostic products. Therefore, the invention provides a method for preparing the yersinia enterocolitica bovine-derived positive serum reference, which comprises the following steps: immunizing a tested bovine by using an immunizing antigen, collecting serum of the immunized tested bovine, and treating the serum to obtain the yersinia enterocolitica bovine-derived positive serum reference. The immune antigen is inactivated yersinia enterocolitica. The Yersinia enterocolitica bovine-derived positive serum reference material fills the blank of the Yersinia enterocolitica bovine-derived positive serum reference material for specific evaluation of a Brucella diagnosis product.