180 results about "Virus type" patented technology

Recombinant influenza virus proteins, including influenza capsomers, subviral particles, virus-like particles (VLP), VLP complexes, and/or any portions of thereof, are provided as a vaccine for influenza viruses. The invention is based on the combination of two vaccine technologies: (1) intrinsically safe recombinant vaccine technology, and (2) highly immunogenic, self-assembled protein macromolecules embedded in plasma membranes and comprised of multiple copies of influenza virus structural proteins exhibiting neutralizing epitopes in native conformations. More specifically, this invention relates to the design and production of functional homotypic and heterotypic recombinant influenza virus-like particles (VLPs) comprised of recombinant structural proteins of human influenza virus type A/Sydney/5/94 (H3N2) and/or avian influenza virus type A/Hong Kong/1073/99 (H9N2) in baculovirus-infected insect cells and their application as a vaccine in the prevention of influenza infections and as a laboratory reagent for virus structural studies and clinical diagnostics.

This invention relates to a transdermal delivery system for treating skin related diseases employing protein nanoparticles to deliver drugs to the keratinocytes and basal membrane cells for the treatment of non-melanoma skin cancer. The current invention presents an effective method for delivering small molecule nucleic acids to the epidermal cells.

A method for using thiazine dyes, especially methylene blue or methylene blue derivatives, in an immediate or controlled release formulation, alone or in combination with low levels of light or other drugs, to selectively inactivate or inhibit hepatitis infection, has been developed. Clinical trial results demonstrate efficacy in a human clinical trial for treatment of hepatitis C by oral administration of methylene blue immediate release formulation, in a dosage of 65 mg twice daily, over a period of at least 100 days. A method for using thiazine dyes, especially methylene blue or methylene blue derivatives, in an immediate or controlled release formulation, along or in combination with low levels of light or other drugs, to prevent or decrease reactivation of viruses, is also described. The preferred class of patient is infected with, or has been exposed to, viruses such as Herpes simplex virus type 1 & 2, Varicella zoster virus, Epstein-Barr virus, Cytomegalovirus, and Herpes virus type 6 & 7, Adenovirus, and Human polyoma viruses, e.g. JC virus and BK virus. In one embodiment the thiazine dye is administered to a patient experiencing symptoms or disease caused by reactivation of a virus. In a preferred embodiment the thiazine dye is administered to a patient at risk for or experiencing symptoms or disease caused by reactivation of a virus, prior to or during immunosuppression or chemotherapy.

Disclosed are a method for preparing and virus type definition test paper for foot and mouth disease and the using method. The test paper comprises O, A, Asia l, C four kinds of serum detecting test paper. The test paper comprises a PVC substrate plate, nitrocellulose membrane, an absorbing pad, a golden mark pad and a sample pad. The PVC substrate plate is installed at the most bottom layer; the nitrocellulose membrane is set on the middle part of the substrate plate; the absorbing pad is stuck on the left end of the nitrocellulose membrane, and the golden mark pad is set on the right end of the nitrocellulose membrane; the sample pad is set on the right upper end of the golden pad. The invention can identify the foot and mouth virus O, A, Asia l and C four serum type.

This invention relates to immortalized avian cells, including those deposited under accession numbers 09070701, 09070702, and 09070703 at the ECACC, and to the use of these cells for the production of viruses. The cells according to the invention are particularly useful for the production of recombinant viral vectors which can be used for the preparation of therapeutic and/or prophylactic compositions for the treatment of animals and more particularly humans.

A PR8 recombinant influenza virus contains an HA and/or NA gene of H1, H3, H4, H5, H6, H7, H9 or H10 subtype influenza virus, and 6 internal genes (PB1, PB2, PA, NP, M and NS genes) of PR8 virus, in which the NS and/or NP gene have the following mutation sites: the NS2 protein encoded by the NS gene has an E67S point mutation, E74S point mutation, or E67S/E74S point mutation, and the NP protein encoded by the NP gene has a G132A point mutation.

The invention discloses a modified enhanced type DC-CIK cell targeting immune cell mass, and a preparation method and application thereof. A method for preparing a DC cell comprises the following steps: performing isolated culture on monocyte, thereby acquiring an immature DC cell; and performing tumor antigen load and induced differentiation culture on the immature DC cell, thereby acquiring a mature DC cell, wherein the monocyte is acquired by separating from a peripheral blood mononuclear cell of a sample. The method for preparing CIK comprises the following steps: performing in vitro differentiation culture on a CIK cell, thereby acquiring an immature CIK cell; preparing a modifying recombined slow virus for performing in vitro modification on the CIK cell; and co-culturing the modified CIK cell and the DC cell, thereby acquiring the mature modified CIK cell (Triumph-DC-CIK), so as to be used for achieving a better tumor immunotherapy effect.

The present invention relates to combination vaccines and methods for treating or preventing diseases or disorders in an animal caused by infection by Bovine Viral Diarrhea Virus (BVDV) Types 1 and 2, Bovine Herpes Virus Type-1 (BHV-1), Bovine Respiratory Syncytial Virus (BRSV), Parainfluenza Virus (PI₃), Campylobacter fetus, Leptospira canicola, Leptospira grippotyphosa, Leptospira hardj-prajitno, Leptospira icterohaemmorrhagiae, Leptospira hardjo-bovis and Leptospira pomona by administering to the animal an effective amount of a combination vaccine. The combination vaccine can be a whole or partial cell inactivated or modified live preparation.

The invention discloses a method for preparing a CAR-T cell from gamma delta T cell derived from umbilical cord blood, the CAR-T cell and an application and also relates to nucleic acid coding a chimeric antigen receptor and a recombinant expression vector expressing the chimeric antigen receptor. The umbilical cord blood is used as a source of the gamma delta T cell, the gamma delta T cell is separated from the umbilical cord blood, after efficient amplification, the T cell is transduced by a lentiviral vector loaded with the chimeric antigen receptor, and the CAR-T cell can be obtained, so that positive tumor cells or other infectious cells which can efficiently or specifically kill related antigens are obtained. The CAR-T cell will play roles in tumor cell therapy and immunotherapy.

Embodiments herein provide for methods, compositions and apparati for detection and/or diagnosis of virus types, subtypes and/or strains. In particular embodiments, the virus is an influenza virus. The apparatus may include a microarray with attached capture probes, designed to bind to oligonucleotides capable of binding at least a portion of a nucleic acid sequence of one or more target genes in a broad array of influenza types, subtypes or strains. The compositions may include isolated nucleic acids as capture probes, target sequences and/or tagged label probes, of use for diagnosis and/or detection of influenza virus.

The invention belongs to the technical field of gene engineering and particularly relates to a method for restraining mesenchymal stem cells from differentiating into fat cells. A purpose of restraining the mesenchymal stem cells from differentiating into the fat cells is realized by the following steps of: constructing recombinant retrovirus plasmids containing ICAM-1 (intercellular cell adhesion molecule) genes, carrying out co-transfection on the packaging cells by the recombinant retrovirus plasmids together with packaging plasmids, collecting relevant viral supernatants, and infecting the mesenchymal stem cells. According to the method for restraining the mesenchymal stem cells from differentiating into the fat cells, the separation method is simple in operation, convenient and practical, the efficiency (more than 90% of ICAM-1 can be highly expressed) for transfecting the mesenchymal stem cells is high, and the obtained mesenchymal stem cells are exuberant in in-vitro multiplication and can be passed down for multiple times (more than 50 generations). Thus, as the method and a culture technique system for restraining mesenchymal stem cells from differentiating into fat cells are established, a foundation for research and application of the mesenchymal stem cells is laid.

The invention relates to the field of animal virology and provides a chicken Newcastle disease virus and its separation method. The virus is code-named SDM01 and the invention provides its separation and preparation method. The inventor carries out biological collection for the strain and its collection number is CCTCC NO:V201109. Through chicken embryo passage, biological characteristics tests such as a hemagglutinin and serum neutralization test, an animal regress test and exogenous virus testing, are carried out. The test results confirm that the virus is the Newcastle disease virus. It isproved through chicken embryo mean death time (MDT), one-day-old chick intracebral pathogenicity index (ICPI) measurement and 6-week-old chick intravenous inoculation pathogenicity index (IVPI) measurement that the Newcastle disease virus is a virulent strain. Positive serum is prepared through separated strain and standard strain to carry out an HI cross neutralization test, a chicken embryo neutralization test, a cell neutralization test, an F and HN gene sequence sequencing and comparison and an immunity protective test. Results confirm that there exist large differences between the Newcastle disease virus SDM01 strain and traditional strains (La Sota strain and Clone30 strain) in both genetic typing and antigenicity.

A method for producing mature hepatocytes having functional hepatic enzyme activity from human pluripotent cells is disclosed. The method includes the step of transferring an external vector comprising the DNA sequence coding for a microRNA having the seed sequence of the microRNA miR-122, the DNA sequence coding for a microRNA having the seed sequence of the microRNA miR-let-7c, a microRNA having the seed sequence of the microRNA miR-122, a microRNA having the seed sequence of the microRNA miR-let-7c, or a combination thereof into one or more fetal hepatocytes. The resulting cells differentiate into mature hepatocytes that exhibit functional hepatic enzyme activity, and can be used in drug metabolism and toxicity testing, in the study of viruses that target hepatic tissue, and as therapeutics.

A related method of maintaining the functional hepatic enzyme activity of primary hepatocytes over time is also disclosed. The method includes the step of transferring an external vector comprising the DNA sequence coding for a microRNA having the seed sequence of the microRNA miR-122 into one or more cultured primary hepatocytes.

The invention relates to a method for implementing strong vivo transplantation of human induced pluripotent stem cell-derived hemopoietic stem progenitor cells. The method is induced by an MLL-AF4 single factor and has the advantages that the method is simple, convenient and efficient, human iPS (induced pluripotent stem) cells can be induced into HSC (hemopoietic stem cells) with strong vivo transplantation effects and multi-strain hemopoietic reconstruction, and many difficulties in the field are broken through. The method mainly has the advantages that the number of factors needing to be added is greatly decreased by induction with the single factor, experimental operation can be remarkably simplified, experimental efficiency is improved, the risk of gene mutation possibly induced by slow virus mediated genome insertion is avoided by a plasmid-mediated inducing method, vivo transplantation efficiency reaches up to 20% or more, medullary system bias of iPSC-HSPC in vivo is corrected,and a medullary system, a lymphoid system and an erythroid can be comprehensively reconstructed in vivo.

A method of treating or preventing graft versus host disease (GVHD) in a subject receiving a graft comprising hematopoietic cells is provided. The method comprises contacting the graft ex vivo with an amount of a Myxoma Virus effective to inhibit proliferation of T lymphocytes in the graft and to treat or prevent GVHD in the host subject following infusion of the graft into the subject. After the contacting of the graft with the Myxoma Virus, the method comprises transplanting the virus-treated graft into the subject.

The invention belongs to the technical field of information safety, and discloses a virus searching and killing method, a virus searching and killing device, equipment and a storage medium. The methodcomprises the following steps: receiving hazard recognition information corresponding to malicious traffic detected by a safety gateway; determining a to-be-monitored IP address according to the hazard recognition information; determining a virus process corresponding to the malicious traffic according to the IP address to be monitored; determining a virus file corresponding to the virus processand a storage path of the virus file according to the process recognition information of the virus process; and clearing the virus process, and searching and killing the virus file according to the storage path. By means of the mode, limitation of virus types is avoided, viruses are searched and killed from the source, and therefore the searching and killing effect on the viruses is greatly improved.

The invention discloses a pig intestinal endothelial cell line for stably expressing a CaS9 protein. The pig intestinal endothelial cell line is characterized in that non-transformed intestinal endothelial cell line IPEC-J2 of a neonatal pig is taken as a host cell and is transfected with a lentiviral expression vector containing CaS9, and a transformant capable of stably expressing the CaS9 protein is obtained by screening of 20mu g/mL of Blasticidin. A construction method of the pig intestinal endothelial cell line comprises the following steps: firstly, constructing the lentiviral expression vector containing the CaS9; secondly, transforming an HEK293T cell; thirdly, transfecting an IPEC-J2 cell; fourthly, performing monoclonal screening. The pig intestinal endothelial cell line IPEC-J2-CaS9 disclosed by the invention can stably express the CaS9 protein, and has a growth curve identical with that of the non-transformed IPEC-J2 cell, and cell morphology identical with that of the IPEC-J2 cell. The pig intestinal endothelial cell line disclosed by the invention has important application value in aspects of researching the growth and differentiation of pig intestinal endothelial cells as well as dealing with the stimulation from exogenous substances.

The invention discloses a one-step RT-PCR (Reverse Transcription-Polymerase Chain Reaction) detection kit for an influenza virus, relating to a PCR kit in the technical field of biology. The kit comprises one-step 5xRT-PCR Buffer, positive control, negative control, three pairs of specific primers and Enzyme Mix; a specific primer AF: 5'-ATTCTAACCGAGGTCGAAACGT-3' and a specific primer AR: 5'-GACAAAGCGTCTACGCTGCAGTC-3' are designed according to a gene M of an influenza virus type A; a specific primer is designed according to a gene NS of an influenza virus type B; and a specific primer is designed according to a gene HA of an influenza virus A type H1N1. The detection kit has a short detection time period, high detection efficiency, high virus detection specificity, high accuracy, higher detection sensitivity than an immunological detection method and high experiment result repeatability, can be applied to qualitative analysis of viruses, and is easy to operate and popularize.

The invention provides a preparation method of a human stem cell with prolonged service life and enhanced angiogenesis. The preparation method comprises the following steps of establishing gene segments of a RNA (ribonucleic acid) testis signal transduction and activating body and angiogenin-1; and recombining the gene segments to a virus expression vector. The service life of the cell can be prolonged by transfection of the human stem cell, the angiogenesis of the human stem cell is improved, and tumorigenesis is avoided. The invention also provides a kit of the human stem cell with the prolonged service life and the enhanced angiogenesis, and the human stem cell is expected to treat ischemic cardiovascular and cerebrovascular diseases well.