Lentivirus vector expression system of luciferase and application thereof

Abstract

The invention relates to a lentivirus vector expression system of luciferase and an application thereof. Particularly speaking, in the invention a luciferase lentivirus expression system with FG12-Luc2 as core plasmid is successfully constructed, and a PVTT-1 cell line from tumor thrombus tissue of liver cancer patients is established by the system. Through the lentivirus expression system, liver cancer tissue is successfully marked; and by experimental methods such as subcutaneous transplantation and orthotopic liver cancer transplantation, a mouse orthotopic transplantation liver cancer metastasis model is established which is suitable for serial passage, is applicable to in-vivo imaging, and can realize real-time monitoring of the growth and metastasis conditions of tumors in the body.

Description

technical field

[0001] The invention relates to a luciferase lentiviral vector expression system and its application. Background technique

[0002] With the continuous development of tumor molecular biology, the research on the mechanism of tumor initiation and progression has made great progress. The discovery of more and more oncogenes and tumor suppressor genes has enabled people to have a relatively clear understanding of the biological events in the early stages of the development of many tumors. However, the late events of tumor development—invasion of peripheral organs and metastasis to distant tissues—remain an unexplored area, and they are the main causes of death in cancer patients. Data show that the death of patients caused by tumor metastasis accounts for 90% of the deaths caused by tumors.

[0003] Tumor metastasis is a complex biological behavior, which includes many related sequential steps such as invasion of blood vessels, transport with blood, and distan...

Images