3477 results about "Virus" patented technology

A platform for characterizing biomolecules, biological tissue sections, viruses, or other particles of interest in a non-invasive, high-throughput, cost-effective, and label-free manner. The platform includes a merged set of imaging modalities including and capable of interferometric microscopy and wide-field or confocal microscopy (such as, for example, a wide-field super-resolution surface enhanced Raman spectroscopy, SERS), although some aspects may be employed with data sets only received from wide-field or confocal microscopy. Related devices, systems, methods, techniques, and variations to the same are also provided.

The invention provides an electrochemical active oxygen supply device for a washing machine and a preparation method. The device comprises a shell, a single closed reaction chamber is formed in the shell, a bifunctional anode electrode and a bifunctional cathode electrode are integrated in the reaction chamber, and a compact and uniform reaction area is formed between the anode electrode and the cathode electrode. When water flows through the reaction area, hydrogen peroxide (H2O2) and / or hydroxyl radicals (OH) are / is electrochemically generated on the surface in situ by accurately adjusting the electrode potential of the difunctional anode, so that instant generation and instant utilization of active oxygen are realized. The active oxygen directly enters a washing system of the washing machine along with water flow, bacteria, viruses and fungi can be efficiently inactivated under the normal-temperature or low-temperature washing condition without additionally adding chemical disinfectants, organic stains and peculiar smell molecules are synchronously degraded, and green, efficient and intelligent healthy washing is achieved.

The invention belongs to the technical field of biology, and particularly relates to a group of paired monoclonal antibodies for resisting H9N2 subtype avian influenza viruses and a surface enhanced Raman spectroscopy rapid detection test strip established based on the antibodies. The paired monoclonal antibody comprises a labeled antibody 1E9 and a capture antibody 3C10 which are prepared by taking hemagglutinin protein of the H9N2 subtype avian influenza virus as antigens and can be specifically combined with the H9N2 subtype avian influenza virus. According to the invention, an SERS (Surface Enhanced Raman Scattering) probe Au-coated Pt-4ATP is used as a marker and is coupled with a labeled antibody 1E9 to prepare a labeled probe, and the labeled probe is paired with a capture antibody 3C10 to establish an SERS immunochromatography test strip. The test strip has a colorimetric and Raman dual interpretation mode, can be used for qualitative and quantitative detection, has the advantages of strong specificity, high sensitivity, good repeatability and stability and the like, is simple and rapid in clinical detection operation, and provides reliable technical support for on-site rapid screening of subtype viruses.

The invention relates to a primer group for detecting pangolin plague virus, a kit and application, and relates to the technical field of gene engineering. The primer group comprises a forward primer ORF1-F and a reverse primer ORF1-R. The forward primer ORF1-F and the reverse primer ORF1-R. On the basis of the primer group, the E gene in the conserved region of the pangolin plague virus can be specifically recognized, so that the primer group is combined with an RAA amplification system and a detection system, and detection is rapidly completed within a short time under the constant-temperature condition.

The application belongs to the technical field of biological detection, and particularly relates to a monoclonal antibody pair for detecting feline immunodeficiency virus p24 protein and application thereof. The monoclonal antibody pair is monoclonal antibody 1D8 and monoclonal antibody 1H10, which can specifically recognize feline immunodeficiency virus p24 protein, and the heavy chain and light chain variable region complementarity determining region sequences of 1D8 and 1H10 are clear, and are respectively shown as SEQ ID NO. 1-12. The antibody pair can effectively avoid cross reaction, and significantly improve the accuracy and sensitivity of detection; the colloidal gold detection test strip constructed based on the antibody pair is simple and fast in operation, is suitable for early antigen detection of FIV infection, solves the problem that existing antibody detection cannot distinguish natural infection and vaccine immunization, and provides a reliable technical means for on-site screening and disease prevention and control of FIV.

The invention discloses an upland cotton GhCRK29 gene as well as an encoding protein and application thereof, the GhCRK29 gene is separated from cotton, the nucleotide sequence of the GhCRK29 gene is as shown in SEQ ID No.1, and the amino acid sequence of the encoding protein of the GhCRK29 gene is as shown in SEQ ID No.2. According to the invention, a virus-induced gene silencing technology and a transgenic overexpression technology are further utilized to carry out preliminary research on the function of the cotton GhCRK29 gene in cotton verticillium wilt resistance, and a result shows that the verticillium wilt resistance of a cotton seedling plant is obviously reduced after the GhCRK29 gene in cotton is silenced; after the GhCRK29 gene is over-expressed in arabidopsis thaliana or cotton, the verticillium wilt resistance of arabidopsis thaliana or cotton is remarkably improved, and the resistance of a GhCRK29 gene forward regulation plant to verticillium dahliae is determined; the invention has application prospects in the aspects of improving the verticillium wilt resistance of plants or cultivating verticillium wilt resistant plant varieties and the like.

The invention provides application of a TaNAC42 gene in regulation and control of wheat stem rust resistance, and belongs to the technical field of gene functions. It is found for the first time that the TaNAC42 gene of wheat is related to the wheat stem rust resistance, the TaNAC42 gene is induced to be silenced through viruses, the wheat stem rust resistance can be enhanced, wheat plants are easier to resist diseases, the TaNAC42 gene is over-expressed through the agrobacterium tumefaciens-mediated transient over-expression technology, the wheat stem rust resistance can be weakened, the wheat plants are more susceptible to diseases, and the wheat plants are more susceptible to diseases. Namely, the TaNAC42 gene can negatively participate in regulating and controlling the resistance of the wheat to the Puccinia graminicola, a new target gene is provided for improved breeding of wheat Puccinia graminicola resistance molecules, and the TaNAC42 gene has important utilization value in wheat production and breeding.

A pathogen nucleic acid aerosol purification device comprises a machine shell, the machine shell comprises a frame, a front panel, a left side plate, a right side plate, a rear baffle and a top cover plate are arranged on the frame, the front panel is an arc-shaped plate with the plate face bent forwards gradually from bottom to top, the rear baffle and the top cover plate are rectangular, and an air inlet is formed in the bottom of the machine shell. The front panel, the left side plate, the right side plate, the rear baffle and the top cover plate define an air channel allowing air to circulate from bottom to top, and a plurality of centrifugal fans are arranged on the upper portion in the air channel side by side at intervals in the left-right horizontal direction. The utility model aims to provide a biological laboratory air purification device which can enable air in a biological laboratory to fully circulate without dead angles, enables the air to be free of eddy current and cross disturbance as far as possible during circulation, and can efficiently disinfect and remove nucleic acid fragments, viruses and bacterial microorganisms in aerosol in the air in the biological laboratory in an extremely short time. And the pathogen nucleic acid aerosol purification device is used for efficiently removing suspended particulate matters in air in a biological laboratory.

Disclosed herein are antibodies or antigen-binding fragments thereof that specifically bind to human CD19. Chimeric antigen receptors and chimeric antigen receptor transgenes comprising an antigen binding domain that specifically binds to human CD19 are also disclosed. Also described herein are immune cells, viral vectors, and other compositions containing the antibodies, the antigen binding fragments, the chimeric antigen receptors, and / or the chimeric antigen receptor transgenes.

The present disclosure provides codon optimized GBA1 polynucleotides encoding a GCase protein, wherein a portion of the coding sequence deviates from the wild type. The disclosure also provides expression constructs, vectors, viral particles or compositions containing the disclosed polynucleotides. In addition, methods and uses of these polynucleotides, expression constructs, vectors, viral particles or compositions are also provided, including the treatment of diseases or conditions associated with GCase deficiency.

One embodiment of the invention discloses a method and device for optimizing network anti-virus indexes. The method comprises following steps: obtaining anti-virus indexes of terminals in a network, wherein the anti-virus index of each terminal in the network comprises at least one kind of information as follows: virus infection and anti-virus capability information; optimizing the anti-virus indexes of terminals in a network by means of the genetic algorithm to obtain the optimized anti-virus indexes of terminals in the network; obtaining the optimized network anti-virus indexes based on the optimized anti-virus indexes of terminals in the network. One embodiment of the invention also discloses a device for optimizing network anti-virus indexes.

This invention discloses a primer and probe set, a kit, and their applications for detecting Staphylococcus aureus L based on real-time fluorescence MIRA technology. The primer and probe set consists of single-stranded DNA sequences 1-3. Based on the aforementioned primer and probe set, this invention establishes and optimizes a real-time fluorescence MIRA detection method for Staphylococcus aureus L. Furthermore, the optimized real-time fluorescence MIRA method is innovatively combined with a simplified nucleic acid extraction method and a portable fluorescence detection device, significantly reducing extraction and time costs while enabling rapid on-site detection of the virus, making it particularly suitable for grassroots units and remote areas. In addition, the real-time fluorescence MIRA detection method for Staphylococcus aureus L established in this invention has advantages such as high specificity, high sensitivity, and simple operation. Moreover, the detection process does not require opening the lid, effectively avoiding aerosol contamination, providing an effective technical means for the differential diagnosis and rapid detection of Staphylococcus aureus L.

The invention discloses a potato Y virus rapid detection method based on RPA-CRISPR / Cas12a-LFA, the RPA-CRISPR / Cas12a-LFA triple technology is adopted, the RPA-CRISPR / Cas12a-LFA triple technology comprises RPA isothermal amplification, Cas12a targeted cutting and LFA visual reading, the potato Y virus can be rapidly and efficiently detected, the sensitivity is high, the specificity is high, and the operation is simple and convenient.

The invention belongs to the technical field of biological medicine, and particularly relates to an engineered cell microvesicle and a delivery system based on the engineered cell microvesicle, the system realizes efficient preparation of 1-5 [mu] m cell microvesicles, and the cell microvesicles have a large space volume and can be used for preparing the cell microvesicles. The carrier can be used for loading and delivery of target protein, polypeptide and recombinase which are specifically expressed in mother cells. The system transfects mother cells through lentivirus transfection or plasmid transfection to further produce cell microvesicles, and the microvesicles can load more goods and inherit membrane proteins of the mother cells, and can also effectively load intracellular proteins to realize effective delivery. By virtue of good structural stability, high immunogenicity and excellent biocompatibility, the cell microvesicle reduces systematic toxic and side effects of a traditional carrier, is expected to become an effective drug delivery system, and has great application potential in the field of gene therapy.

The invention discloses macromolecular antibacterial artificial leather and a preparation method thereof, and relates to the technical field of artificial leather preparation. The preparation method comprises the following steps: preparing zinc-loaded silicon dioxide with amino grafted on the surface and quaternized polyether polyurethane containing an epoxy group, mixing the zinc-loaded silicon dioxide and the quaternized polyether polyurethane to prepare antibacterial protection slurry, and bonding the zinc-loaded silicon dioxide and the quaternized polyether polyurethane through ring-opening reaction of the amino and the epoxy group during curing to form a cross-linked network, so as to obtain the antibacterial protection layer with antibacterial property, flexibility and wear resistance; zinc ions have an obvious inhibiting effect on bacteria such as gram-positive bacteria and gram-negative bacteria and fungi such as candida albicans, quaternary ammonium salt has an obvious inhibiting effect on influenza viruses, and the zinc ions and the quaternary ammonium salt have a synergistic effect, so that the antibacterial range of the artificial leather is wider, the inhibiting force on germs is stronger, and the antibacterial property of the artificial leather is further improved.

And genetic determinants conferring improved ToBRFV resistance. The present invention relates to a tomato plant resistant to tomato brown crinkled fruit virus (ToBRFV), comprising in the genome of said tomato plant the following combination: a resistant quantitative trait locus on chromosome 8 (QTL) genetically infiltrated from solanum tomentosum and conferring resistance to ToBRFV, and a resistant quantitative trait locus on chromosome 8 (QTL) genetically infiltrated from solanum tomentosum and conferring resistance to ToBRFV; and at least one QTL or Tm-1 gene on chromosome 11, which QTL, when homozygically present, confers resistance to ToBRFV to a plant leaf surface, said plant having improved resistance to ToBRFV. The invention also relates to corresponding seeds and cells, as well as various methods and uses for providing improved ToBRFV resistance based on the genetic determinant combination.

The invention relates to the technical field of biology, and particularly discloses application of OsDjA5 protein or a coding gene thereof to regulation and control of resistance of plants to southern rice black-streaked dwarf viruses. The research finds that after the expression quantity of the OsDjA5 gene is reduced, the resistance of rice to the southern rice black-streaked dwarf virus is enhanced. The invention further provides application of the OsDjA5 protein or the coding gene thereof, or a biological material containing the coding gene thereof in regulating and controlling the resistance of plants to the southern rice black-streaked dwarf virus. The invention provides a new method capable of improving the resistance of the plants to the southern rice black-streaked dwarf virus, and a new thought is provided for creating new varieties of disease-resistant plants.

The invention provides a high-throughput detection and bioinformation analysis and identification method for a human ring virus group, the method comprises the whole process from reference database construction, sample processing, high-throughput sequencing to bioinformation analysis, and the technical scheme specifically comprises the following steps: step 1, constructing a human ring virus high-quality reference database; 2, performing high-throughput sequencing on the metagenome of the human sample; and step 3, a ring virus group biological information analysis process.

Methods to edit genes by administering a chimeric nuclease to a cell or organism without the use of a viral vector.

The invention belongs to the technical field of external diagnosis devices, and particularly relates to a memristor for adenovirus concentration detection and a preparation method of the memristor. The memristor comprises a bottom electrode layer, a metal oxide layer, a functional layer and an upper electrode layer which are sequentially formed from bottom to top in the thickness direction, the bottom electrode layer is an FTO conductive glass layer, the metal oxide layer is tantalum pentoxide, the functional layer is a gelatin-beta cyclodextrin composite film, and the upper electrode layer is a conductive electrode layer. The gelatin-beta cyclodextrin composite film with the memristive effect serves as a functional layer, after the film makes contact with adenoviruses, the film adsorbs virus particles, then the unique resistance change characteristic of the memristor is caused, the memristive behavior of the memristor is further fed back, and the memristive effect of the memristor is improved. The method is used for recording and analyzing the electrochemical or bio-electricity signal characteristics of adenovirus solutions with different concentrations, the adenovirus solutions with different concentrations are effectively distinguished according to the electric signal difference of the adenovirus solution and the control solution, the effect is obvious, and the repeatability is good.

The invention provides a macro virus group sequencing library construction method for removing host ribosome RNA through an enzyme method and a kit, and belongs to the technical field of biology. The method comprises the following steps: hybridizing total RNA (Ribonucleic Acid) of a sample with a DNA (Deoxyribose Nucleic Acid) probe aiming at host ribosome RNA to form a DNA / RNA hybrid chain; rNase H is used for digesting RNA in the hybrid chain, and DNase I is used for digesting the remaining DNA probe in the system; purifying to obtain virus RNA (Ribonucleic Acid); carrying out fragmentation treatment on the virus RNA; carrying out reverse transcription on the fragmented RNA to synthesize a first chain cDNA, then synthesizing a second chain cDNA, and carrying out terminal repair and dA tail addition to obtain double-chain cDNA; connecting the linker with the dT tail with the double-chain cDNA; and carrying out PCR amplification and purification on the connection product to obtain the macro virus group sequencing library. According to the construction method and the kit, the problem that the full-length sequence of the virus cannot be obtained from a low-load sample can be solved.

An engineered producer cell comprising an inactivating mutation in one or more endogenous REP binding sites is provided, as are methods for producing the engineered producer cell and using the engineered producer cell to produce a recombinant viral vector and reduce producer cell genomic DNA contamination of a recombinant adeno-associated virus vector preparation.

The present invention provides synthetic peptide compounds and uses thereof for therapy and diagnostics of complement-mediated diseases, such as inflammatory diseases, autoimmune diseases, and microbial and bacterial infections; and non-complement-mediated diseases, such cystic fibrosis and various acute diseases. The invention is directed to modifications of a synthetic peptide of 15 amino acids from the Polar Assortant (PA) peptide, which is a scrambled peptide derived from human Astrovirus protein. In some embodiments, the invention is directed to peptide compounds that are peptide mimetics, peptide analogs and / or synthetic derivatives of PA (e.g., sarcosine derivatives) having, for example, internal peptide substitutions, and modifications, including PEGylation at the N-terminus and C-terminus. The invention further provides methods of selecting at least one synthetic peptide for treating various conditions.

The invention discloses an insect antibacterial peptide nutrient solution as well as a preparation method and application thereof, and belongs to the technical field of biology. Insect protein (fly maggot powder / hermetia illucens) and plant fiber (bran) are subjected to enzymolysis and then subjected to a pH gradient chelation process (5.0-7.0-8.5), metal ions (Fe < 2 + > / Mn < 2 + > / Zn < 2 + >) are added in stages for chelation, the chelation degree is larger than or equal to 91.4%, lentinan and alginate oligosaccharide are added for synergistic interaction, and finally the insect antibacterial peptide nutrient solution is obtained. Experiments prove that after the insect antibacterial peptide nutrient solution prepared by the invention is used for carrying out root irrigation treatment on plants, tomato yellow leaf curl virus or tomato spotted wilt virus can be remarkably prevented and treated, and the insect antibacterial peptide nutrient solution has high efficiency and persistence and conforms to the development direction of green plant protection.

The present invention provides peptide compounds that regulate the complement system and methods of using these compounds. The invention is an isolated, purified peptide of 30 amino acids derived from human astrovirus protein, called CP1. The invention is directed to peptide compounds that are peptide mimetics, peptide analogs and / or synthetic derivatives of CP1 having, for example, internal peptide deletions and substitutions, deletions and substitutions at the N-terminus and C-terminus, and that are able to regulate complement activation. The invention further provides pharmaceutical compositions of therapeutically effective amounts of the peptide compounds and a pharmaceutically acceptable carrier, diluent, or excipient for treating a disease or condition associated with complement-mediated tissue damage.

The present disclosure provides materials and methods related to the purification of viral vectors. In particular, the present disclosure provides peptides, compositions, adsorbents, and related methods, capable of removing process-related impurities (e.g., host cell proteins, nucleic acids, and media components) and product-related impurities (e.g., product fragments, product aggregates, and inactive forms derived from product degradation by or association with other species in the cell culture harvest) from samples during the production and purification of adenovirus.

The invention discloses a kit for detecting a muscovy duck reovirus based on a RAA-CRISPR / Cas12a technology. Belongs to the technical field of Muscovy duck reovirus detection. The invention aims to detect the Muscovy duck reovirus. The invention provides a kit for detecting a muscovy duck reovirus based on an RAA-CRISPR / Cas12a technology. The detection kit comprises an RAA isothermal amplification system and a CRISPR / Cas12a detection system, the method effectively solves the problems that a traditional detection technology is long in time consumption, depends on precise instruments and is complex in operation to detect the pain points on the breeding site.

Provided is a method for large-scale preparation of a purified preparation of a recombinant lentiviral vector at the GMP grade. The method comprises: (a) providing raw material feed liquid to be purified that comprises recombinant viral vectors; (b) carrying out a microfiltration treatment on the feed liquid to obtain a microfiltered filtrate comprising the recombinant viral vectors; (c) optionally concentrating the filtrate to obtain a concentrated filtrate; (d) purifying the filtrate obtained in the previous step by means of chromatography to obtain a crude pure product comprising the recombinant viral vectors; and (e) subjecting the crude pure product obtained in the previous step to liquid exchange and elaborate purification to obtain the purified recombinant viral vectors.

The invention discloses a recombinant adeno-associated virus vector for targeted delivery of VEGF-C (vascular endothelial growth factor-C) and application of the recombinant adeno-associated virus vector in promoting endocardial differentiation to treat coronary heart disease. The rAAV vector comprises transgene for coding VEGF-C protein or VEGF-C protein functional variant and a tissue-specific promoter operably connected to the transgene, wherein the tissue specific promoter is capable of driving the transgene to be specifically expressed in the heart endothelial cells. According to the treatment method developed by the invention, specific expression of the Vegfc gene in the heart is driven by using an ICAM2 promoter with targeting to endothelial cells, and a remarkable curative effect is shown in a myocardial infarction model mouse. The therapeutic scheme provides a new effective strategy for intervention and treatment of myocardial infarction and chronic heart failure, and has the advantages of high safety, long gene expression duration, extremely low immune response to a host and the like.

The invention provides a rotating machine fault diagnosis method for improving H5N1-VMD-MFWA noise reduction, and belongs to the technical field of mechanical fault diagnosis, and the method comprises the steps: obtaining vibration signals of a rotating machine under normal and fault conditions; taking the minimum envelope entropy as a fitness function, and performing parameter optimization on variational mode decomposition by adopting an improved avian influenza virus algorithm to obtain an optimal parameter [K, alpha] combination; variational mode decomposition is carried out on the vibration signal based on the optimal parameter, and K intrinsic mode function components are obtained through decomposition; and according to the correlation coefficient, the kurtosis and the envelope entropy of each component, a comprehensive evaluation index is constructed through a multi-feature weighted attention mechanism, each component is endowed with a weight, a signal is reconstructed, and the reconstructed signal is subjected to window segmentation and input into a convolutional neural network model to complete fault diagnosis. According to the method, the fault diagnosis accuracy is effectively improved, and a guarantee is provided for safe operation of mechanical equipment.