6449 results about "Virus" patented technology

A regenerative filter that includes a filter housing having inlet and outlet zones; a fluid path provided between the inlet and outlet zones; a plurality of filter elements each having an outer surface filter media applied thereto and functioning to filter particulate or contaminants from the fluid path; and a tube sheet that is supported across the filter housing, that is disposed just before the outlet zone and that provides the support for the plurality of filter elements. The plurality of filter elements are disposed in an array and includes bridging members or elements that connect between adjacent filter elements, and that forms with the filter elements, a closed interstitial space between adjacent filter elements for liquid flow; a nanoscale barrier on interior surfaces, including that of the regenerative media itself, providing a mechanism to disrupt the cell wall of microscopic viruses and organisms.

A regenerative filter that includes a filter housing having inlet and outlet zones; a fluid path provided between the inlet and outlet zones; a plurality of filter elements each having an outer surface filter media applied thereto and functioning to filter particulate or contaminants from the fluid path; and a tube sheet that is supported across the filter housing, that is disposed just before the outlet zone and that provides the support for the plurality of filter elements. The plurality of filter elements are disposed in an array and includes bridging members or elements that connect between adjacent filter elements, and that forms with the filter elements, a closed interstitial space between adjacent filter elements for liquid flow; a nanoscale barrier on interior surfaces, including that of the regenerative media itself, providing a mechanism to disrupt the cell wall of microscopic viruses and organisms.

The disclosure relates to ribonucleic acid (RNA) vaccines and combination vaccines, as well as methods of using the vaccines and compositions comprising the vaccines. Specific embodiments relate to RNA betacoronavirus vaccines formulated in a lipid nanoparticle.

The invention provides a virus-removing ultrafiltration membrane and a preparation method thereof, and belongs to the technical field of membrane separation. According to the preparation method of the ultrafiltration membrane, a hydrophilic polymer and a bulk polymer are blended to prepare a membrane casting solution, the ultrafiltration membrane is prepared on the basis of a double-layer homogeneous compounding method and a phase inversion method, and the obtained integrated ultrafiltration membrane comprises a separation layer with a compact skin layer and a pre-filtration supporting layer with a complete sponge structure, and provides high mechanical strength and toughness at the same time; the separation layer and the supporting layer form a hourglass-shaped structure with effective aperture continuously changing in a gradient mode, and no obvious interface or layering phenomenon exists. The ultra-filtration membrane is clear in gradient structure, the upper surface is loose, the lower surface is compact, the structures are fused more tightly, no obvious interface exists, the filtration resistance is reduced, and the protein permeability is improved. And the flux and the maximum treatment volume are improved and the treatment time is shortened while the relatively high virus rejection rate and the relatively low protein adsorption capacity are maintained.

The invention discloses a kit for detecting tomato brown crinkled fruit virus based on enzyme-mediated dual-amplification nucleic acid amplification and a detection method. The detection system comprises a primer pair DNA (deoxyribonucleic acid) upstream primer (F5) and a DNA downstream primer (R6) for amplifying the tomato brown crinkled fruit virus movement protein gene, a probe (RNA primer (RNA1)) for detecting the MP gene, a nucleic acid amplification group enzyme system (recombinase, single-chain binding protein, polymerase and ATP (adenosine triphosphate) energy regeneration enzyme), a signal amplification group enzyme system (positioning enzyme and cutting enzyme), activating enzyme NTP, a buffer solution and RNase-free Water. The system composed of the freeze-drying protective agent is prepared into the pre-subpackaged freeze-dried balls. The kit disclosed by the invention can realize DNA detection of aM concentration, and besides rapid qualitative detection of the tomato brown crinkled fruit virus, early screening of the tomato brown crinkled fruit virus can also be realized due to high detection sensitivity.

The invention provides a nucleic acid combination product, and a kit and a method for detecting human infectious diarrhea pathogenic viruses. The nucleic acid combination product comprises a composition of one or more groups of primer pairs and detection probes. The nucleic acid combination product has no cross reaction on near-source non-target pathogens and non-target pathogens with the same sampling parts and similar clinical symptoms, each target can reach 10 copies / reaction, and the nucleic acid combination product has high sensitivity and specificity.

The disclosure relates to compositions and methods for the preparation, use, and / or formulation of adeno-associated virus capsid protein variants.

The invention belongs to the technical field of nano antibodies, and particularly relates to a nano antibody specifically bound with AAV9 and application of the nano antibody. The nano antibody provided by the invention can be specifically combined with the AAV9 adeno-associated virus without being combined with other AAV serotypes such as AAV2, AAV5 and AAV8, so that high-sensitivity and high-specificity detection of the AAV9 adeno-associated virus is realized. In practical application, the nano-antibody coupled solid-phase carrier provided by the invention is adopted as an affinity filler, the AAV9 adeno-associated virus can be effectively captured and mildly eluted, the virus purification and recovery effects are effectively improved, and the nano-antibody coupled solid-phase carrier is suitable for high-purity recovery of the AAV9 adeno-associated virus.

The invention relates to the technical field of virus whole genome sequencing, and discloses a method for virus whole genome sequencing, which comprises the following steps: S1, extracting a sample genome, operating according to a specification of a commodity kit, extracting RNA (Ribonucleic Acid) of the sample genome, and performing library construction after reverse transcription and targeted amplification. According to the method for sequencing the whole genome of the virus, the whole genome sequence of the virus can be obtained through the sequencing method, and compared with the current detection which only stays at negative and positive detection, the whole genome sequence can be obtained on the basis through the method for sequencing, epidemiological traceability is carried out, and meanwhile the sequencing cost can be effectively reduced.

A platform for characterizing biomolecules, biological tissue sections, viruses, or other particles of interest in a non-invasive, high-throughput, cost-effective, and label-free manner. The platform includes a merged set of imaging modalities including and capable of interferometric microscopy and wide-field or confocal microscopy (such as, for example, a wide-field super-resolution surface enhanced Raman spectroscopy, SERS), although some aspects may be employed with data sets only received from wide-field or confocal microscopy. Related devices, systems, methods, techniques, and variations to the same are also provided.

The invention discloses a nested PCR (Polymerase Chain Reaction) amplification primer for an internally-running sheep disease virus, which comprises a first-round upstream primer, a first-round downstream primer, a second-round upstream primer and a second-round downstream primer, the nucleotide sequence of the first-round upstream primer is as shown in SEQ ID NO: 1, the nucleotide sequence of the second-round upstream primer is as shown in SEQ ID NO: 3, and the nucleotide sequence of the second-round downstream primer is as shown in SEQ ID NO: 4. The nucleotide sequences of the first round of downstream primer and the second round of downstream primer are as shown in SEQ ID NO: 2. The kit has the advantages of excellent specificity and sensitivity, low cost and good stability.

The invention discloses an integrated fluorescence detection kit and a testing method thereof.The integrated fluorescence detection kit comprises an upper cover assembly, a lysate tube assembly, a main body assembly, a diluent tube assembly and a decorative cover, and the lower end of the upper cover assembly is connected with the lysate tube assembly and the diluent tube assembly; the side wall of the upper cover assembly is connected with the upper part of the main body assembly, and the main body assembly is nested in the decorative cover; a reagent system in the kit comprises an RT-RAA reaction system and a CRISPR reaction system which are stored in the detection kit at normal temperature in the form of freeze-dried microspheres and closed liquid, and a lysis solution and a diluent are released through the lysis solution tube assembly and the diluent tube assembly and react in the main body assembly. According to the invention, the problems that the existing fluorescence detection operation process is complicated, the instrument is not portable and depends on professional analysis and the like are solved, and the rapid, portable and user-friendly detection on the virus is realized based on the CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) detection system.

The invention provides an electrochemical active oxygen supply device for a washing machine and a preparation method. The device comprises a shell, a single closed reaction chamber is formed in the shell, a bifunctional anode electrode and a bifunctional cathode electrode are integrated in the reaction chamber, and a compact and uniform reaction area is formed between the anode electrode and the cathode electrode. When water flows through the reaction area, hydrogen peroxide (H2O2) and / or hydroxyl radicals (OH) are / is electrochemically generated on the surface in situ by accurately adjusting the electrode potential of the difunctional anode, so that instant generation and instant utilization of active oxygen are realized. The active oxygen directly enters a washing system of the washing machine along with water flow, bacteria, viruses and fungi can be efficiently inactivated under the normal-temperature or low-temperature washing condition without additionally adding chemical disinfectants, organic stains and peculiar smell molecules are synchronously degraded, and green, efficient and intelligent healthy washing is achieved.

The invention discloses an RAA-CRISPR / Cas12a detection system of a circle virus avian origin type 1 and application of the RAA-CRISPR / Cas12a detection system, and relates to the technical field of microbiological detection. The detection system comprises an RAA amplification primer pair, crRNA, an FQ-ssDNA probe, an FB-ssDNA probe and an LbCas12a nuclease, and is characterized in that the detection system comprises an RAA amplification primer pair; the RAA amplification primer pair comprises an upstream primer of which the nucleotide sequence is shown as SEQ ID NO.5 and a downstream primer of which the nucleotide sequence is shown as SEQ ID NO.8; the nucleotide sequence of the crRNA is as shown in SEQ ID NO. 11. The RAA-CRISPR / Cas12a detection kit established by adopting the detection system developed by the invention has the advantages of high efficiency, high specificity and high sensitivity, can be used for on-site rapid detection of GyVg1, and provides a powerful diagnostic tool with a cost advantage for a basic-level working environment.

A traditional Chinese medicine composition for treating abnormal lung function and laryngitis caused by the influenza virus; the traditional Chinese medicine composition comprising as a primary formula of active ingredients seven medicinal / edible source materials composed of: Siraitia grosvenorii, Cinnamomum cassia, Prunella vulgaris, Houttuynia cordata, Platycodon grandiflorus, Gardenia jasminoides and Glycyrrhiza uralensis. The foundation of the primary formula is supplemented by a secondary formula of seven medicinal / edible source materials consisting of: Citrus reticulata (mandarin orange), Eriobotrya japonica leaves, Angelica dahurica, Arctium lappa root, Piper nigrum, Phragmites australis root and Syzygium aromaticum, and different preparations are prepared. The traditional Chinese medicine combined formula can be a combined formula of source herbs, and can likewise be a combined formula of extracts thereof, and can likewise be a combined formula of source herbs and extracts. The traditional Chinese medicine composition has a significant therapeutic effect on symptoms such as sore throat, itchy throat cough, swelling and pain in the throat, pharyngeal discomfort, etc. resulting from influenza-caused pneumonia such as abnormal lung function and difficulty breathing, cough, lung nodules, ground glass opacity in the lungs, pulmonary fibrosis, interstitial lung disease and laryngitis, and has a certain relieving, progression-stopping and disease condition control and treatment effects on chronic obstructive emphysema.

The invention relates to the technical field of infectious disease on-site simulation, in particular to a respiratory infectious disease on-site epidemic AI simulation experiment system based on environment evaluation. Performing virus region diffusion analysis based on a virus propagation risk assessment result, the environment data and the contact condition of nearby facilities, performing path virus risk assessment based on a path virus region diffusion analysis result, a personnel evacuation condition and a virus attenuation condition, and acquiring an evacuation path according to a path virus risk assessment result. By evaluating the risk of the virus in the path, the path with relatively low virus transmission risk can be accurately identified, and the path is selected for personnel evacuation in the period of infectious disease outbreak, so that the contact opportunity between evacuated personnel and the virus can be reduced to the greatest extent, the possibility of infectious disease infection is effectively reduced, and the life health and safety of the public are guaranteed.

The invention relates to a water-soluble carbon chain substance for adjusting transmembrane transport and fluidity of a cell membrane, and preparation and application thereof. The water-soluble carbon chain substance comprises a carbon chain originally having certain water solubility, and the structure of the water-soluble carbon chain substance comprises at least one or more hydrophobic carbon chain parts and one or more hydrophilic groups or residues; the hydrophobic carbon chain part is coupled with water-soluble molecules to form a high-hydrophilicity compound; the water-soluble carbon chain can be used for preventing and treating infections including viruses, bacteria and fungi; anti-aging; the occurrence of neurodegenerative diseases such as Alzheimer's disease is prevented and reduced; by reducing the non-specific phagocytosis of the nano-drug by cells, the effect of the nano-drug on a target focus is enhanced.

The invention discloses a hybridoma cell strain, a monoclonal antibody, a linear epitope antigen and application thereof, and relates to the field of genetic engineering, in particular to a hybridoma cell strain, a monoclonal antibody, a linear epitope antigen and application thereof. The preservation number of the hybridoma cell strain P27-A1 is CGMCC (China General Microbiological Culture Collection Center) No. 46352. The hybridoma cell strain P27-A1 is prepared from feline leukemia virus p27, and a monoclonal antibody secreted by the hybridoma cell strain P27-A1 recognizes a linear epitope antigen of feline leukemia virus p27 protein; the kit for the feline leukemia virus p27 comprises the monoclonal antibody A1 secreted by the hybridoma cell strain P27-A1. The monoclonal antibody secreted by the hybridoma cell strain can greatly improve the sensitivity of the p27 protein for detecting feline leukemia virus. The invention provides the monoclonal antibody secreted by the hybridoma cell strain for identification, and the monoclonal antibody can be used for preparing a diagnostic kit such as a colloidal gold test strip.

The invention relates to the technical field of HPV therapeutic vaccines, in particular to an MATV51 recombinant adenovirus vector, application, a preparation method and an HPV 16 / 18 / 52 therapeutic vaccine. The MATV51 recombinant adenovirus vector comprises nucleotide sequences used for coding E6 and E7 antigens of HPV16, HPV18 and HPV52, and further comprises a nucleotide sequence used for coding CD40L. A nucleotide sequence of the recombinant adenovirus vector is designed aiming at an HPV16 / 18 / 52E6-E7 protein antigen and contains a nucleotide sequence for coding CD40L, and the expressed fusion protein takes CD40L as an adjuvant; the HPV 16 / 18 / 52 therapeutic vaccine can cover genotypes of three HPV viruses at the same time, and has good immune effect and memory effect, so that the effect of the vaccine is effectively improved, and the application range is wide.

The invention belongs to the technical field of biology, and particularly relates to a group of paired monoclonal antibodies for resisting H9N2 subtype avian influenza viruses and a surface enhanced Raman spectroscopy rapid detection test strip established based on the antibodies. The paired monoclonal antibody comprises a labeled antibody 1E9 and a capture antibody 3C10 which are prepared by taking hemagglutinin protein of the H9N2 subtype avian influenza virus as antigens and can be specifically combined with the H9N2 subtype avian influenza virus. According to the invention, an SERS (Surface Enhanced Raman Scattering) probe Au-coated Pt-4ATP is used as a marker and is coupled with a labeled antibody 1E9 to prepare a labeled probe, and the labeled probe is paired with a capture antibody 3C10 to establish an SERS immunochromatography test strip. The test strip has a colorimetric and Raman dual interpretation mode, can be used for qualitative and quantitative detection, has the advantages of strong specificity, high sensitivity, good repeatability and stability and the like, is simple and rapid in clinical detection operation, and provides reliable technical support for on-site rapid screening of subtype viruses.

The invention discloses a kit for visually detecting an H5 subtype avian influenza virus based on an RPA-CRISPR / Cas12b system and application of the kit, and belongs to the technical field of molecular detection. The kit comprises an RT-RPA (Reverse Transcription-Recombinase Polymerase Amplification) primer pair and sgRNA1 The RT-RPA primer pair comprises a forward primer with a nucleotide sequence as shown in SEQ ID NO.1 and a reverse primer with a nucleotide sequence as shown in SEQ ID NO.2; and the nucleotide sequence of the sgRNA1 is as shown in SEQ ID NO. 7. According to the kit, nucleic acid amplification and detection are synchronously completed through a tubular reaction system, the detection sensitivity reaches 1 copy / mu L, the coincidence rate with fluorescent RT-PCR is 100%, and no cross reaction exists on H7, H9, NDV and IBV. The kit does not need a complex instrument, is suitable for rapid screening on a basic level site, and provides an efficient solution for prevention and control of avian influenza.

The invention discloses an RcWRKY29 gene and application of the RcWRKY29 gene in enhancing the resistance of Chinese roses to gray mold. The nucleotide sequence of the RcWRKY29 gene is shown as SEQ ID NO: 1. Researches find that the gene is specifically and highly expressed in Chinese rose petals, and the expression quantity is remarkably increased within 24-48 hours after botrytis cinerea infection. After RcWRKY29 expression is instantaneously inhibited through a virus-induced gene silencing technology, the scab area of Chinese rose petals is increased, and the disease resistance is remarkably reduced; otherwise, overexpression of the gene can significantly enhance disease resistance and reduce scab area and cell damage. The invention provides a recombinant vector containing RcWRKY29, an engineering bacterium and a detection kit, and develops a method for enhancing the disease resistance of Chinese rose based on an agrobacterium-mediated transformation technology. The invention provides a new target for Chinese rose gray mold resistance molecular breeding, can effectively reduce postharvest disease loss, and has important economic value and popularization and application prospects.

The embodiment of the invention discloses human-derived recombinant III-type collagen as well as a preparation method and application thereof. The amino acid sequence of the human-derived recombinant III-type collagen comprises a basic repetitive unit, and the amino acid sequence of the basic repetitive unit is as shown in SEQ ID NO.4. The amino acid sequence of the basic repetitive unit is as shown in SEQ ID NO.3. The amino acid sequence of the basic repetitive unit is as shown in SEQ ID NO.4. The amino acid sequence of the human recombinant III type collagen is shown as SEQ ID NO.3. The invention also discloses a preparation method of the human recombinant III type collagen. According to the invention, a human recombinant III-type collagen gene with the coding molecular weight of 60kD is designed, an escherichia coli expression strain is constructed and expressed, the yield of the human recombinant III-type collagen is increased through amplification culture on a fermentation tank, and the protein purity is improved by virtue of strategies such as ultrafiltration primary purification and affinity chromatography. The human-derived recombinant III-type collagen prepared by the invention avoids potential hidden dangers such as animal diseases or viruses and immunological rejection caused by a traditional extraction method, has excellent medical performance, can effectively control the production cost and the product quality by virtue of a fermentation preparation process, and is also easy to realize large-scale production.

The invention provides a CRISPR (clustered regularly interspaced short palindromic repeats) / Cas-based kit for isothermal rapid visual detection of H1N1 influenza virus and mycoplasma pneumoniae and a detection method thereof. The kit and the detection method provided by the invention can be used for timely, sensitively and specifically detecting a nucleic acid target in a sample. The invention provides a lateral flow analysis (LFA) detection platform of a three-stage amplification system integrating recombinase polymerase amplification (RPA), CRISPR / Cas12a and catalytic hairpin assembly (CHA) circulation, the detection platform is used for sensitively and accurately detecting mycoplasma pneumoniae DNA (MP DNA) and influenza A virus subtype H1N1RNA (H1N1RNA), and compared with other researches based on an LFA-CRISPR / Cas12a system, the detection platform has the advantages that the detection efficiency is greatly improved, and the detection cost is reduced. According to the kit and the detection method provided by the invention, nucleic acids of two pathogens can be visually detected on a single LFA at the same time, and the sensitivity is high.

The invention provides siRNA for targeted regulation and control of HBV gene expression and application of the siRNA. The double-stranded RNAi agent comprises an antisense strand and a sense strand complementary to the antisense strand forming a double-stranded region, and the nucleotide sequence of the antisense strand is as shown in SEQ ID NO: 8, or the nucleotide sequence of the antisense strand is a modified sequence of the sequence as shown in SEQ ID NO: 8. Cell and animal experiment results show that the double-stranded RNAi agent provided by the invention can significantly reduce the expression of one or more HBV genes and block the life cycle of viruses, and can be used for developing drugs for treating HBV gene expression related diseases.

The invention provides a molecular marker for detecting pepper light mottle virus L-series resistance genes and application of the molecular marker, and belongs to the technical field of genetic engineering, the molecular marker comprises SNP1 and SNP2, the SNP1 is a base T at the 2574th site of a yellow lantern pepper genome sequence with the NCBI login number of AB523370, and the SNP2 is a base T at the 2832nd site of the yellow lantern pepper genome sequence with the NCBI login number of AB523370; the specific gene sequence is a specific gene sequence of an L-series resistance gene L1c type gene, and the specific gene sequence is a basic group CA at the 2872 site to the 2873 site, a basic group TT at the 2877 site to the 2878 site, a basic group TG at the 2882 site to the 2883 site and a basic group AGA at the 2885 site to the 2887 site of the yellow lantern pepper with the NCBI accession number being AB523374. By utilizing the molecular marker and the specific sequence, the L-series resistance gene types of the yellow lantern pepper and the annual pepper can be rapidly and accurately detected, and the molecular marker can be used for molecular marker-assisted selective breeding.

The invention belongs to the field of biological medicines, and particularly discloses application of DNAJB6 in preparation of medicines for preventing or treating myocardial infarction (MI). In-vivo and in-vitro experiments find that the expression of DNAJB6 protein in an MI model is remarkably reduced, and the over-expression of DNAJB6 can improve the cardiac function of a mouse after MI, relieve myocardial fibrosis and inhibit hypoxia-induced myocardial apoptosis; and cell apoptosis is aggravated by low expression of DNAJB6. The overexpression of the DNAJB6 by using the myocardial specific adeno-associated virus (AAV) can be used as a new strategy for preventing and treating the MI, and an experimental basis is provided for developing a drug for targeting the DNAJB6.

The invention relates to a primer group for detecting pangolin plague virus, a kit and application, and relates to the technical field of gene engineering. The primer group comprises a forward primer ORF1-F and a reverse primer ORF1-R. The forward primer ORF1-F and the reverse primer ORF1-R. On the basis of the primer group, the E gene in the conserved region of the pangolin plague virus can be specifically recognized, so that the primer group is combined with an RAA amplification system and a detection system, and detection is rapidly completed within a short time under the constant-temperature condition.

The invention relates to application of an MYZAP overexpression vector in preparation of a medicine for preventing and treating MIRI, and belongs to the technical field of biological medicine. In order to solve the problem that application of gene drugs in prevention and treatment of myocardial ischemia reperfusion injury is limited, the invention provides application of an MYZAP gene overexpression recombinant vector in preparation of drugs for prevention and treatment of myocardial ischemia reperfusion injury, and the MYZAP gene overexpression recombinant vector is an adeno-associated virus vector AAV9-MYZAP for overexpressing MYZAP. Experiments prove that MYZAP overexpression can improve the cardiac function after myocardial ischemia reperfusion, reduce the occurrence rate of arrhythmia and improve the functions of sodium ion channels, potassium ion channels and calcium ion channels of myocardial cells. On the basis, the invention further provides a medicine for preventing and treating myocardial ischemia reperfusion injury, a new strategy is provided for gene therapy of myocardial ischemia reperfusion injury, and the medicine has a wide clinical application prospect.

A triple droplet type digital PCR (ddPCR) detection method for the severe fever with thrombocytopenia syndrome virus (SFTSV), spotted heat group rickettsia (SFGR) and tsutsugamushi Ot is established. According to the method, specific primers and probes are designed based on an SFTSV L gene, an SFGR 17Kd gene and an Ot 47Kd htrA gene, the reaction temperature and the concentration of the primers and probes are optimized to establish an SFTSV, SFGR and Ot triple ddPCR reaction system, and the specificity, sensitivity and repeatability of the triple ddPCR reaction system are analyzed. According to results, the optimal primer concentration and probe concentration of triple digital PCR are 600nmol / L and 250nmol / L respectively, and the optimal annealing temperature is 60 DEG C; the correlation coefficients of the triple ddPCR SFTSV, the triple ddPCR SFGR and the triple ddPCR Ot standard curve are 0.98, 0.99 and 0.98 respectively, and the lowest detection lower limits are 0.59 copies / mu L, 0.46 copies / mu L and 0.36 copies / mu L respectively. According to the invention, the SFTSV, SFGR and Ot triple digital PCR detection method is established, the sensitivity is high, the specificity is strong, and quantitative detection of three pathogens in SFTS suspected cases can be realized.